1. |
Naoko Okibe, Kaito Hayashi, Keishi Oyama, Kazuhiko Shimada, Yuji Aoki, Takahiro Suwa, Tsuyoshi Hirajima, "Bioleaching of Enargite/Pyrite-Containing “Dirty” Concentrate and Arsenic Immobilization.", Minerals, 2022.04. |
2. |
Ryohei Nishi, Santisak Kitjanukit, Taiki Kondo, Naoko Okibe, "Simultaneous arsenic and iron oxidation for one-step scorodite crystallization using Mn oxide.", Materials Transactions, doi:10.2320/matertrans.MT-M2021120, 11, 2021.10.  |
3. |
Takahiro Matsumoto, Idol Phann, Naoko Okibe, "Biogenic Platinum Nanoparticles’ Production by Extremely Acidophilic Fe(III)-Reducing Bacteria.", Minerals, doi:org/10.3390/ min11111175, 11, 1175, 2021.10.  |
4. |
Keishi Oyama, Kyohei Takamatsu, Kaito Hayashi, Yuji Aoki, Shigeto Kuroiwa, Tsuyoshi Hirajima, Naoko Okibe, "Carbon-assisted bioleaching of chalcopyrite and three chalcopyrite/enargite-bearing complex concentrates.", Minerals, doi.org/10.3390/min11040432, 11, 4, 432, 2021.04.  |
5. |
Ryohei Nishi, Santisak Kitjanukit, Kohei Nonaka, Naoko Okibe, “Oxidation of arsenite by self-regenerative bioactive birnessite in a continuous flow column reactor.”, Hydrometallurgy, 10.1016/j.hydromet.2020.105416, 196, 105416, 2020.09, Naturally occurring manganese (Mn) oxide, biogenic birnessite ((Na, Ca, K)0.5 MnIII, IV2O4·1.5 H2O), is involved in the geochemical cycling of variety of metals including arsenic (As). This natural reaction was exploited in this study to develop a sustainable oxidation treatment process of As(III) to the less soluble (and less toxic) As(V). It is known that the birnessite surface becomes passivated during As(III) oxidation, which quickly decreases its reactivity. The cycle batch test and the following XANES (X-ray absorption near-edge structure) analysis in this study confirmed that combining chemical As(III) oxidation by birnessite with simultaneous birnessite regeneration by Mn-oxidizing microorganisms (Pseudomonas sp. SK3) can avoid passivation of MnIII-precipitates and enables continuous As(III) oxidation while increasing the AOS (average oxidation state) of birnessite. This chemical/microbiological synergism was observed for the As(III) concentration range of 0.2–0.5 mM with 0.1% birnessite, wherein no net Mn loss from birnessite was noticed for complete As(III) oxidation. The continuous column test was run for 40 days at a HRT (hydraulic retention time) of 3 h by feeding a 0.2 mM As(III) solution. The As(III) oxidation efficiency of >98% was consistently achieved while strictly controlling the Mn2+ dissolution throughout the test period. This study concluded that by taking advantage of a robust microbial Mn-oxidizing activity, the use of “bioactive” birnessite realizes self-sustainable oxidation of As(III), without necessitating additional feed of oxidant birnessite, Mn2+ ions or organics..  |
6. |
Keishi Oyama, Kazuhiko Shimada, Jun-ichiro Ishibashi, Keiko Sasaki, Hajime Miki, Naoko Okibe, “Catalytic mechanism of activated carbon-assisted bioleaching of enargite concentrate.”, Hydrometallurgy, 10.1016/j.hydromet.2020.105417, 196, 105417, 2020.09, The catalytic mechanism of activated carbon-assisted bioleaching of enargite concentrate (enargite 37.4%; pyrite 47.3%) was investigated by employing microbiological, electrochemical and kinetic studies. By using moderately thermophilic microorganisms at 45 degrees C, the final Cu dissolution was improved from 36% to 53% at 0.2% (w/v) activated carbon. An excess activated carbon addition showed an adverse effect. The enargite mineral itself favored higher solution redox potential (E-h) for solubilization. However, the dissolution of co-existing pyrite, which also favors high E-h, immediately hindered enargite dissolution through the passivation effect. The surface of activated carbon functioned as an electron mediator to couple RISCs oxidation and Fe3+ reduction, so that elevation of the E-h level was controlled by offsetting microbial Fe3+ regeneration. As long as the E-h level was suppressed at < 700 mV, the dissolution of pyrite was largely avoided, enabling a steady and continuous dissolution of the enargite mineral through the surface chemical reaction model. When the E-h-control by activated carbon becomes no longer sustainable and the E-h hits 700 mV, rapid pyrite dissolution was initiated and the surface chemical reaction of enargite dissolution came to an end. Arsenic species dissolved from enargite was constantly immobilized with an efficiency of 75-90% as amorphous ferric arsenate. However, the sudden initiation of pyrite dissolution also triggered the re-solubilization of ferric arsenate. Therefore, the sustainable E-h-controlling effect was shown to be critical to enable longer Cu dissolution from enargite as well as stabilization of As precipitates..  |
7. |
Melisa Pramesti Dewi, Himawan Tri Bayu Murti Petrus, Naoko Okibe, “Recovering secondary REE value from spent oil refinery catalysts using biogenic organic acids.”, Catalysts, 10.3390/catal10091090, 10, 9, 1090-15, 2020.09, Spent catalysts produced by oil refinery industries are regarded as an important secondary source for valuable metals. In particular, spent fluid catalytic cracking (FCC) catalysts represent a potential source for rare earth elements (REEs). This study aimed to exploit the leachability of spent FCC catalysts as a secondary source for La, by using an alternative organic acid lixiviant produced under optimized fungal fermentation conditions. The first chemical leaching tests revealed that citric acid (>100 mM) is a comparable alternative lixiviant to conventional inorganic acids (1 M) and that the La dissolution behavior changed significantly with different types of organic acids. The initial fungal fermentation conditions (e.g., inoculum level, substrate concentration, pH) largely affected the resultant biogenic acid composition, and its manipulation was possible in order to almost solely ferment citric acid (~130 mM) while controlling the production of unwanted oxalic acid. The performance of actual biogenic acids (direct use of cell-free spent media) and artificially reconstituted biogenic acids (a mixture of chemical reagents) was nearly identical, achieving a final La dissolution of ~74% at a pulp density of 5%. Overall, the microbiological fermentation of organic acids could become a promising approach to supply an efficient and environmentally benign alternative lixiviant for REE scavenging from spent FCC catalyst wastes..  |
8. |
Haruki Noguchi, Naoko Okibe, “The role of bioleaching microorganisms in saline water leaching of chalcopyrite concentrate.”, Hydrometallurgy, 10.1016/j.hydromet.2020.105397, 195, 105397, 2020.08, In order to tackle the dual challenge of utilizing highly refractory chalcopyrite (CuFeS2) while saving scarce freshwater resources, this study aimed to systematically understand the individual role of chemical lixiviant and bioleaching microorganisms in the complex Fe3+-Cu2+-SO42−-Cl− chalcopyrite leaching system. In general freshwater bioleaching conditions, the Eh level sharply increased, and the “high-Eh-bioleaching” became the major leaching driving force. In this case, the lowest Cu yield was obtained. The chalcopyrite leaching reaction responded differently to different salinity levels. At a low salinity of 0.5% NaCl, chemical Cl−-leaching effect resulted in a higher Cu yield than the fresh-water “high-Eh-bioleaching” system. The growth of tested microbes was observed at 0.5% NaCl, but partial deactivation of microbial Fe-oxidation suppressed the Eh level. Under this condition, synergism between the chemical Cl−-leaching effect and the “low-Eh-bioleaching” effect was found. At a high salinity of 2% NaCl, on the other hand, no active cell growth was observed, and thus pre-grown cells were used to mimic the presence of Cl−-tolerant cells. Chemical Cl−-leaching readily proceeded at 2% NaCl at low Eh, but quickly ceased upon the depletion of H+. The presence of bioleaching cells somewhat slowed down the speed of chemical Cl−-leaching, but the acid depletion was alleviated by microbial acid generation. Chemical Cl−-leaching, which favors low Eh condition, was the main driving force for chalcopyrite leaching at 2% NaCl. Therefore, the activity of Cl−-tolerant S-oxidizer alone, rather than mixed Fe- and S-oxidizing consortium, was shown to play a critical role in maximizing the chalcopyrite dissolution..  |
9. |
Naoko Okibe, Ryohei Nishi, Yuta Era, Takeharu Sugiyama, “The effect of heterogeneous seed crystals on arsenite removal as biogenic scorodite.”, Materials Transactions, doi.org/10.2320/matertrans.M-M2019858, 61, 2, 387-395, 2019.12.  |
10. |
Naoko Okibe, Yuken Fukano, “Bioremediation of highly toxic arsenic via carbon-fiber-assisted indirect As(III) oxidation by moderately-thermophilic, acidophilic Fe-oxidizing bacteria.”, Biotechnology Letters, 41, 1403-1413, 2019.10.  |
11. |
Santisak Kitjanukit, Keiko Sasaki, Naoko Okibe, “Production of highly catalytic, archaeal Pd(0) bionanoparticles using Sulfolobus tokodaii.”, Extremophiles, 23, 5, 549-556, 2019.09.  |
12. |
Santisak Kitjanukit, Kyohei Takamatsu, Naoko Okibe, “Natural attenuation of Mn(II) in metal refinery wastewater: Microbial community structure analysis and isolation of a new Mn(II)-oxidizing bacterium Pseudomonas sp. SK3.”, Water, 11, 507, 2019.03.  |
13. |
Intan Nurul Rizki, Yu Tanaka, Naoko Okibe, “Thiourea bioleaching for gold recycling from e-waste.”, Waste Management, 84, 158-165, 2019.02.  |
14. |
Yusei Masaki, Katsutoshi Tsutsumi, Naoko Okibe, “Iron redox transformation by the thermo-acidophilic archaea from the genus Sulfolobus.”, Geomicrobiology Journal, doi.org/10.1080/01490451.2018.1465491, 35, 9, 757-767, 2018.10.  |
15. |
Masahito Tanaka, Keiko Sasaki, Naoko Okibe, “Behavior of sulfate ions during biogenic scorodite crystallization from dilute As(III)-bearing acidic waters.”, Hydrometallurgy, 180, 144-152, 2018.07.  |
16. |
Yusei Masaki, Tsuyoshi Hirajima, Keiko Sasaki, Hajime Miki, Naoko Okibe, “Microbiological redox potential control to improve the efficiency of chalcopyrite bioleaching.”, Geomicrobiology Journal, 35, 8, 648-656, 2018.04.  |
17. |
Keishi Oyama, Kazuhiko Shimada, Jun-ichiro Ishibashi, Hajime Miki, Naoko Okibe, “Silver-catalyzed bioleaching of enargite concentrate using moderately thermophilic microorganisms.”, Hydrometallurgy, 177, 197-204, 2018.03.  |
18. |
Intan Nurul Rizki, Naoko Okibe, “Size-controlled production of gold bionanoparticles using the extremely acidophilic Fe(III)-reducing bacterium, Acidocella aromatica.”, Minerals, 8, 81, 1-11, 2018.02.  |
19. |
Masahito Tanaka, Naoko Okibe, “Factors to enable crystallization of environmentally stable bioscorodite from dilute As(III)-contaminated waters.”, Minerals, 8, 23, 1-16, 2018.01.  |
20. |
Naoko Okibe, Daisuke Nakayama, Takahiro Matsumoto, “Palladium bionanoparticles production from acidic Pd(II) solutions and spent catalyst leachate using acidophilic Fe(III)-reducing bacteria.”, Extremophiles, 21, 6, 1091-1100, 2017.10. |
21. |
Masahito Tanaka, Tsuyoshi Hirajima, Keiko Sasaki, Naoko Okibe, “Optimization of bioscorodite crystallization for treatment of As(III)-bearing wastewaters.”, Solid State Phenomena, 262, 555-558, 2017.08. |
22. |
Keishi Oyama, Tsuyoshi Hirajima, Keiko Sasaki, Hajime Miki, Naoko Okibe, “Mechanism of silver-catalyzed bioleaching of enargite concentrate.”, Solid State Phenomena, 262, 273-276, 2017.08. |
23. |
Santisak Kitjanukit, Kyohei Takamatsu, Kenji Takeda, Satoshi Asano, Naoko Okibe, “Manganese removal from metal refinery wastewater using Mn(II)-oxidizing bacteria.”, Solid State Phenomena, 262, 673-676, 2017.08. |
24. |
Yuta Era, Tsuyoshi Hirajima, Keiko Sasaki, Naoko Okibe, “Microbiological As(III) oxidation and immobilization as scorodite at moderate temperatures.”, Solid State Phenomena, 262, 664-667, 2017.08. |
25. |
Naoko Okibe, Shiori Morishita, Masahito Tanaka, Keiko Sasaki, Tsuyoshi Hirajima, Kazuhiro Hatano, Atsuko Ohata, "Bioscorodite crystallization using Acidianus brierleyi: Effects caused by Cu(II) present in As(III)-bearing copper refinery wastewaters", Hydrometallurgy, 10.1016/j.hydromet.2016.07.003, 168, 121-126, 2017.03, © 2016 Elsevier B.V. This study investigated the effect caused by Cu(II) in synthetic As(III)-bearing copper refinery wastewaters, on microbial scorodite (FeAsO4·2H2O) formation using the thermo-acidophilic Fe(II)- and As(III)-oxidizing archaeon, Acidianus brierleyi. Microbial Fe(II) oxidation and cell growth became only marginal in the presence of 8–16 mM Cu(II), with its As(III) oxidation ability being severely inhibited. Consequently, scorodite formation was disabled by Cu(II) addition. However, feeding scorodite seed crystals readily alleviated Fe(II)- and As(III)-oxidation ability of Ac. brierleyi at 8 mM Cu(II), forming crystalline scorodite within 24 days in shake flasks. Zeta potential analysis indicated cell attachment to the scorodite seed crystal surface, implying its role in providing the immediate support for microbial colonization and enabling more robust microbial reactions. Most of Cu(II) was neither adsorbed nor co-precipitated and remained in the solution phase during scorodite crystallization, with or without the presence of seed crystals. Addition of seed crystals at 0.015, 0.03, 0.075 and 0.15% resulted in As immobilization of 96, 97, 97 and 98%, respectively, by day 24. This study demonstrated that despite of its inhibitory effect on Ac. brierleyi cells, scorodite can still be crystallized in the presence of Cu(II) by feeding scorodite seeds from synthetic copper refinery As(III)-bearing wastewaters.. |
26. |
Naoko Okibe, Shiori Morishita, Masahito Tanaka, Keiko Sasaki, Tsuyoshi Hirajima, Kazuhiro Hatano, Atsuko Ohata, “Bioscorodite crystallization using Acidianus brierleyi: Effects caused by Cu(II) present in As(III)-bearing copper refinery wastewaters.”, Hydrometallurgy, 168, 121-126, 2016.07. |
27. |
Naoko Okibe, Masashi Maki, Daisuke Nakayama, Keiko Sasaki, “Microbial recovery of vanadium by the acidophilic bacterium, Acidocella aromatica.”, Biotechnology Letters, 10.1007/s10529-016-2131-2, 38, 1475-1481, 2016.05. |
28. |
Yusei Masaki, Katsutoshi Tsutsumi, Shin-ichi Hirano, Naoko Okibe, “Microbial community profiling of the Chinoike Jigoku (“Blood Pond Hell”) hot spring in Beppu, Japan: isolation and characterization of Fe(III)-reducing Sulfolobus sp. strain GA1.”, Research in Microbiology, 10.1016/j.resmic.2016.04.011, 167, 7, 595-603, 2016.05. |
29. |
Yuniati Mutia Dewi, Keitaro Kitagawa, Tsuyoshi Hirajima, Hajime Miki, Naoko Okibe, Keiko Sasaki, “Suppression of pyrite oxidation in acid mine drainage by carrier microencapsulation using liquid product of hydrothermal treatment of low-rank coal, and electrochemical behavior of resultant encapsulating coatings.”, Hydrometallurgy, 83-93, 2015.12. |
30. |
Yusei Masaki, Shin-ichi Hirano, Naoko Okibe, “Microbial community structure analysis of Blood Pond Hell hot spring in Japan and search for metal-reducing microbes.”, Advanced Materials Research, 10.4028/www.scientific.net/AMR.1130.45, 1130, 45-49, 2015.11. |
31. |
Naoko Okibe, Shiori Morishita, Masahito Tanaka, Tsuyoshi Hirajima, Keiko Sasaki, “Effect of Cu(II) on bio-scorodite crystallization using Acidianus brierleyi.”, Advanced Materials Research, 10.4028/www.scientific.net/AMR.1130.101, 1130, 101-104, 2015.11. |
32. |
Widi Astuti, Tsuyoshi Hirajima, Keiko Sasaki, Naoko Okibe, “Utilization of metabolic citric acid from Aspergillus niger using corn starch in the nickel leaching of Indonesian saprolitic ore.”, Advanced Materials Research, 10.4028/www.scientific.net/AMR.1130.251, 1130, 251-254, 2015.11. |
33. |
Masahito Tanaka, Yuta Yamaji, Yuken Fukano, Kazuhiko Shimada, Junichiro Ishibashi, Tsuyoshi Hirajima, Keiko Sasaki, Mitsuru Sawada, Naoko Okibe, “Biooxidation of gold-, silver, and antimony-bearing highly refractory polymetallic sulfide concentrates, and its comparison with abiotic pre-treatment techniques.”, Geomicrobiology Journal, 10.1080/01490451.2014.981645, 32, 6, 538-548, 2015.07. |
34. |
Naoko Okibe, Kiyomasa Sueishi, Mikoto Koga, Yusei Masaki, Tsuyoshi Hirajima, Keiko Sasaki, Shinichi Heguri, Satoshi Asano, “Selenium (Se) removal from copper refinery wastewater using a combination of zero-valent iron (ZVI) and Se(VI)-reducing bacterium, Thaurea selenatis.”, Materials Transactions, 10.2320/matertrans.M2014457, 56, 6, 889-894, 2015.05. |
35. |
Yusei Masaki, Tsuyoshi Hirajima, Keiko Sasaki, Naoko Okibe, “Bioreduction and immobilization of hexavalent chromium by the extremely acidophilic Fe(III)-reducing bacterium Acidocella aromatica strain PFBC.”, Extremophiles, 10.1007/s00792-015-0733-6, 19, 2, 495-503, 2015.03. |
36. |
Naoki Higashidani, Takashi Kaneta, Nobuyuki Takeyasu, Shoji Motomizu, Naoko Okibe, Keiko Sasaki, “Speciation of arsenic in a thermoacidophilic iron-oxidizing archaeon, Acidianus brierleyi, and its culture medium by inductively coupled plasma-optical emission spectroscopy combined with flow injection pretreatment using an anion-exchange mini-column”, Talanta, 122, 240-245, 2014.03. |
37. |
Naoko Okibe, Masaharu Koga, Shiori Morishita, Masahito Tanaka, Shinichi Heguri, Satoshi Asano, Keiko Sasaki, Tsuyoshi Hirajima, “Microbial formation of crystalline scorodite for treatment of As(III)-bearing copper refinery process solution using Acidianus brierleyi”, Hydrometallurgy, http://dx.doi.org/10.1016/j.hydromet.2014.01.008, 143, 34-41, 2014.03. |
38. |
Naoko Okibe, Masaharu Koga, Keiko Sasaki, Tsuyoshi Hirajima, Shinichi Heguri, Satoshi Asano, “Simultaneous oxidation and immobilization of arsenite from refinery waste water by thermoacidophilic iron-oxidizing archaeon, Acidianus brierleyi”, Minerals Engineering, 48, 126-134, 2013.07. |
39. |
Naoko Okibe, Masashi Maki, Keiko Sasaki, Tsuyoshi Hirajima, “Mn(II)-oxidizing activity of Pseudomonas sp. strain MM1 is involved in the formation of massive Mn sediments around Sambe hot springs in Japan”, Materials Transactions, 54, 2027-2031, 2013.07. |
40. |
Naoko Okibe, Nobuaki Suzuki, Masayuki Inui, Hideaki Yukawa, "pCGR2 copy number depends on the par locus that forms a ParC-ParB-DNA partition complex in Corynebacterium glutamicum", Journal of Applied Microbiology, 115, 495-508, 2013.04. |
41. |
Keiko Sasaki, Yoshitaka Uejima, Atsushi Sakamoto, Yu Qianqian, Junichiro Ishibashi, Naoko Okibe, Tsuyoshi Hirajima, “Geochemical and microbiological analysis of Sambe hot springs, Shimane prefecture, Japan”, Resource Geology, 63, 2, 155-165, 2013.03. |
42. |
T Hirajima, Y Aiba, M Farahat, N Okibe, K Sasaki, T Tsuruta, K Doi, “Effect of microorganisms on flocculation of quarts”, International Journal of Mineral Processing, 102-103, 107-111, 2012.01. |
43. |
T Hirajima, Y Aiba, M Farahat, N Okibe, K Sasaki, T Tsuruta, K Doi, “Flocculation of quarts by microorganisms”, Biohydrometallurgy; biotech key to unlock mineral resources value (G Qiu, T. jiang, W. Qin, X. Liu, Y. Tang and H. Wang eds). Central South University, Changsha, China, 441-444, 2011.09. |
44. |
K Sasaki, M Koga, K Takatsugi, N Okibe, T Hirajima, S Asano, S Heguri , “Immobilization of arsenite from the refining discharge using Acidianus brierleyi in the presence of pyrite.” , Biohydrometallurgy; biotech key to unlock mineral resources value (G Qiu, T. jiang, W. Qin, X. Liu, Y. Tang and H. Wang eds). Central South University, Changsha, China, 1106-1108, 2011.09. |
45. |
N Okibe, DB Johnson, “A rapid ATP-based method for determining active microbial populations in mineral leach liquors” , Hydrometallurgy, 108, 3-4, 195–198, 2011.07. |
46. |
N Okibe, N Suzuki, M Inui, H Yukawa, “Efficient markerless gene replacement in Corynebacterium glutamicum using a new temperature-sensitive plasmid” , Journal of Microbiological Methods, 85, 2, 155-163, 2011.05. |
47. |
N Okibe, N Suzuki, M Inui, H Yukawa, “Antisense RNA-mediated plasmid number control in pCG1-family plasmids, pCGR2 and pCG1, in Corynebacterium glutamicum” , Microbiology, 156, Pt 12, 3609-3623, 2010.12. |
48. |
N Okibe, N Suzuki, M Inui, H Yukawa, “Isolation and evaluation of two strong, carbon source-inducible promoters from Corynebacterium glutamicum” , Letters in Applied Microbiology, 50, 2, 173-180, 2010.02. |
49. |
DB Johnson, P Bacelar-Nicolau, N Okibe, A Thomas, KB Hallberg, “Ferrimicrobium acidiphilum gen. nov., sp. nov., and Ferrithrix thermotolerans gen. nov., sp. nov.: heterotrophic iron-oxidizing, extremely acidophilic Actinobacteria.” , International Journal of Systematic and Evolutionary Microbiology., 59, 5, 1082-1089, 2009.05. |
50. |
N Suzuki, K Watanabe, N Okibe, Y Tsuchida, M Inui, H Yukawa, “Identification of new secreted proteins and secretion of heterologous amylase by C. glutamicum.” , Applied Microbiology and Biotechnology, 82, 3, 491-500, 2009.03. |
51. |
K Watanabe, Y Tsuchida, N Okibe, H Teramoto, N Suzuki, M Inui, H Yukawa, “Scanning the Corynebacterium glutamicum R genome for high-efficiency secretion signal sequences.” , Microbiology, 155, 3, 741-750, 2009.03. |
52. |
DB Johnson, N Okibe, K Wakemana, L Yajie, “Effect of temperature on the bioleaching of chalcopyrite concentrates containing different concentrations of silver” , Hydrometallurgy, 94, 1-4, 42-47, 2008.11. |
53. |
DB Johnson, L.Yajie, N Okibe, ““Bioshrouding”―a novel approach for securing reactive mineral tailings.” , Biotechnology Letters, 30, 3, 445-449, 2008.03. |
54. |
MA Ghauri, N Okibe, DB Johnson, "Attachment of acidophilic bacteria to solid surfaces: The significance of species and strain variations.” , Hydrometallurgy, 85, 72-80, 2007.03. |
55. |
DB Johnson, N Okibe, KB Hallburg, “Differentiation and identification of iron-oxidizing acidophilic bacteria using cultivation techniques and amplified ribosomal DNA restriction enzyme analysis.”, Journal of Microbiological Methods, 60, 3, 299-313, 2005.03. |
56. |
N Okibe, DB Johnson, “Bioleaching of pyrite by defined mixed populations of moderately thermophilic acidophiles in pH-controlled bioreactors.” , Biohydrometallurgy; a sustainable technology in evolution (Tsezos, M., Hatzikioseyian, A. and Remoudaki, E., eds.). National Technical University of Athens, Zografou, Greece, 165-173, 2004.09. |
57. |
N Okibe, DB Johnson, “Biooxidation of pyrite by defined mixed cultures of moderately thermophilic acidophiles in pH-controlled bioreactors: significance of microbial interactions.” , Biotechnology and Bioengineering, 87, 5, 574-583, 2004.09. |
58. |
DB Johnson, N Okibe, FF Roberto, “Novel thermo-acidophilic bacteria isolated from geothermal sites in Yellowstone National Park: physiological and phylogenetic characteristics.”, Archives of Microbiology, 180, 1, 60-68, 2003.07. |
59. |
N Okibe, M Gericke, KB Hallberg, DB Johnson, “Enumeration and characterization of acidophilic microorganisms isolated from a pilot plant stirred-tank bioleaching operation.” , Applied and Environmental Microbiology, 69, 4, 1936-1943, 2003.04. |
60. |
N Okibe, DB Johnson, “Toxicity of flotation chemicals to moderately thermophilic bioleaching microorganisms.” , Biotechnology Letters, 24, 23, 2011-2016, 2002.12. |
61. |
DB Johnson, P Bacelar-Nicolau, N Okibe, A Yahya, KB Hallberg, “Role of pure and mixed cultures of Gram-positive eubacteria in mineral leaching.”, Biohydrometallurgy: Fundamentals, Technology and Sustainable Development. (Ciminelli, V. S. T. and Garcia Jr., O., eds.) Amsterdam, Elsevier, 11A, 461-470, 2001.09. |
62. |
N Okibe, DB Johnson, “Bioleaching of pyrite by defined mixed cultures of moderately thermophilic acidophiles.”, Biohydrometallurgy: Fundamentals, Technology and Sustainable Development. (Ciminelli, V. S. T. and Garcia Jr., O., eds.) Amsterdam, Elsevier, 11A, 443-451, 2001.09. |
63. |
N Okibe, K Amada, S Hirano, M Haruki, T Imanaka, M Morikawa, S Kanaya, “Gene cloning and characterization of aldehyde dehydrogenase from a petroleum-degrading bacterium, strain HD-1.”, Journal of Bioscience and Bioengineering, 88, 1, 7-11, 1999.07. |