Kyushu University Academic Staff Educational and Research Activities Database
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Atsushi Shimada Last modified date:2019.12.23

Associate Professor / Division of Structural Biology
Department of Molecular and Structural Biology
Medical Institute of Bioregulation


Graduate School


E-Mail
Homepage
http://vsb.bmr.kyushu-u.ac.jp/VSB/index.html
Division of Structural Biology, Medical Institute of Bioregulation .
Phone
092-642-6833
Fax
092-642-6833
Academic Degree
Ph.D
Country of degree conferring institution (Overseas)
No
Field of Specialization
structural biology, biochemistry, cell biology
ORCID(Open Researcher and Contributor ID)
0000-0002-4108-770X
Total Priod of education and research career in the foreign country
02years00months
Outline Activities
Cell biological phenomena, such as cell migration and endocytosis, are dynamic processes accompanied with membrane and/or actin cytoskeletal remodeling. These processes are regulated by cellular signaling pathways, which link extracellular signals to corresponding morphological changes of the cell. A number of proteins with various functions are involved in these pathways, which include those with membrane remodeling and/or actin remodeling activities. However, the detailed functions of many of these proteins at atomic resolution are still not fully understood. To reveal novel mechanisms exerted by these proteins, which support the maintenance of the activities of eukaryotic cells, structural and functional analyses are being performed using mainly X-ray crystallography and biochemical techniques, such as isothermal titration calorimetry. Cell biological analyses to test hypotheses derived from structural and functional analyses are also being performed in collaboration with other groups.
Research
Research Interests
  • Crystallography of the complex between a novel plant actin-binding domain and actin
    keyword : plant, actin, FH2 domain
    2012.07.
  • Structural biology of oligosaccharyltransferases
    keyword : oligosaccharyltransferase, N-glycosylation
    2011.10.
  • Structural basis of vesicle formation in endocytosis
    keyword : X-ray crystallography, endocytosis, BAR domain, EFC/F-BAR domain
    2004.07.
Current and Past Project
  • Structural and functional studies of a protein family involved in ER-Golgi and Golgi-endosome trafficking and regulation of ER-mitochondria contact sites.
Academic Activities
Reports
1. Atsushi Shimada and Shigeyuki Yokoyama, Crystal structure of the EFC/F-BAR domain -mechanism of membrane invagination in endocytosis-, SPring-8 Research Frontiers 2007, 2008.07.
Papers
1. Hanawa-Suetsugu K, Itoh Y, Ab Fatah M, Nishimura T, Takemura K, Takeshita K, Kubota S, Miyazaki N, Wan Mohamad Noor WNI, Inaba T, Nguyen NTH, Hamada-Nakahara S, Oono-Yakura K, Tachikawa M, Iwasaki K, Kohda D, Yamamoto M, Kitao A, Shimada A, Suetsugu S., Phagocytosis is mediated by two-dimensional assemblies of the F-BAR protein GAS7, Nature Communications, 10.1038/s41467-019-12738-w, 10, 4763, 2019.10.
2. Atsushi Shimada, Atsuko Yamaguchi, Daisuke Kohda, Structural basis for the recognition of two consecutive mutually interacting DPF motifs by the SGIP1 μ homology domain., Sci. Rep., 29, 6, 19565, 2016.01.
3. Shimada, A., Takano, K., Shirouzu, M., Hanawa-Suetsugu, K., Terada, T., Toyooka, K., Umehara, T., Yamamoto, M., Yokoyama, S. and Suetsugu, S., Mapping of the basic amino-acid residues responsible for tabulation and cellular protrusion by EFC/F-BAR domain of pacsin2/Syndapin II, FEBS Lett., 584, 6, 1111-1118, 2010.03.
4. Shimada, A., Niwa, H., Tsujita, K., Suetsugu, S., Nitta, K., Hanawa-Suetsugu, K., Akasaka, R., Nishino, Y., Toyama, M., Chen, L., Liu, Z.J., Wang, B.C., Yamamoto, M., Terada, T., Miyazawa, A., Tanaka, A., Sugano, S., Shirouzu, M., Nagayama, K., Takenawa, T. and Yokoyama, S. , Curved EFC/F-BAR domain dimers are joined end to end into a filament for membrane invagination in endocytosis
, Cell, 129, 4, 761-772, 2007.05.
5. Shimada, A., Nyitrai, M., Vetter, I.R., Kuhlmann, D., Bugyi, B., Narumiya, S., Geeves, M.A. and Wittinghofer, A., The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization
, Mol. Cell, 13, 4, 511-522, 2004.02.
6. Shimada, A., Nureki, O., Goto, M., Takahashi, S. and Yokoyama S, Structural and mutational studies of the recognition of the arginine tRNA-specific major identity element, A20, by arginyl-tRNA synthetase
, Proc. Natl. Acad. Sci. USA, 98, 24, 13537-13542, 2001.11.
7. Sekine, S., Nureki, O., Shimada, A., Vassylyev, D.G. and Yokoyama S, Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase, Nat. Struct. Biol., 8, 3, 203-206, 2001.03.
8. Fukai, S., Nureki, O., Sekine, S., Shimada, A., Tao, J., Vassylyev, D.G. and Yokoyama, S., Structural basis for double-sieve discrimination of L-valine from L-isoleucine and L-threonine by the complex of tRNA(Val) and valyl-tRNA synthetase, Cell, 103, 5, 793-803, 2000.11.
9. Nureki, O., Vassylyev, D.G., Tateno, M., Shimada, A., Nakama, T., Fukai, S., Konno, M., Hendrickson, T.L., Schimmel, P. and Yokoyama, S., Enzyme structure with two catalytic sites for double-sieve selection of substrate, Science, 280, 5363, 578-582, 1998.04.
Presentations
1. 嶋田 睦, エンドサイトーシス関連細胞質タンパク質の構造から迫るクラスリン重合機構 (Insights into clathrin assembly from the structures of cytosolic endocytic proteins), 第55回 日本生物物理学会年会, 2017.09.
2. 嶋田 睦, 山口 淳子, 神田 大輔, Structural basis for the recognition of two consecutive mutually interacting DPF motifs by the SGIP1 μ homology domain and its implications for the mechanism of affinity enhancement by the increase in the number of consecutive DPF motifs, The 42nd Naito Conference, “In the Vanguard of Structural Biology: Revolutionizing Life Sciences”, 2016.10.
3. Atsuko Yamaguchi, Daisuke Kohda, Atsushi Shimada, Structural basis for the recognition of tandem DPF motifs by the SGIP1 μ homology domain, International Symposium between Kyushu University Post-Global Centers of Excellence Program and School of Biomedical Sciences, Monash University, 2014.02.
Membership in Academic Society
  • Japanese Biochemical Society
  • Protein Science Society of Japan
  • The Molecular Biology Society of Japan
  • The Biophysical Society of Japan
Educational
Educational Activities
Teaching undergraduate and graduate students research skills including biochemical and structural biological techniques.