Kyushu University Academic Staff Educational and Research Activities Database
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Rie Wakabayashi Last modified date:2023.09.27

Graduate School
Undergraduate School

 Reseacher Profiling Tool Kyushu University Pure
Academic Degree
Country of degree conferring institution (Overseas)
Field of Specialization
Supramolecular Chemistry
ORCID(Open Researcher and Contributor ID)
Total Priod of education and research career in the foreign country
Research Interests
  • Development of scaffolds to assemble proteins
    keyword : proteins, enzymatic reaction, scaffold
  • Development of transcutaneous vaccine by biomolecular delivery through the skin
    keyword : protein, peptide, transcutaneous delivery, vaccine
  • Peptide Self-Assembled Nanomaterials for Biological Applications
    keyword : peptide, self-assembly, biomaterials
Academic Activities
1. Rie Wakabayashi, Wahyu Ramadhan, Kousuke Moriyama, Masahiro Goto, Noriho Kamiya, Poly(ethylene glycol)-based biofunctional hydrogels mediated by peroxidase-catalyzed cross-linking reactions, Polymer Journal, 10.1038/s41428-020-0344-7, 2020.01, Biofunctional hydrogels prepared by a peroxidase, especially horseradish peroxidase (HRP), serve as an excellent class of materials or platform for the development of cellular scaffolds because their biocompatibility and mild and tunable reaction conditions provide them with desirable properties. In this focus review, we summarize our decade of research into HRP-mediated fabrication of biofunctional hydrogels and their applications, in particular cell culture scaffolds. A brief overview of potential substrates employed in HRP and improvement of the HRP hydrogelation system from the initial step until the hydrogen peroxide removal stage in an effort to meet environmental standards is discussed. We highlight our system and describe its biocompatibility and ability to functionalize molecules to support biofabrication by increasing cellular adhesiveness, retaining growth factor affinity, and finally accelerating the formation of two- and three-dimensional multicellular architectures. In the last section, we outline the adoption of hydrogelation as a self-standing, compartmentalized reaction system, i.e., the use of hydrogel marble to conduct cell-free biosynthesis. We believe that this HRP-mediated hydrogel system offers great potential not only as a cell culture scaffold but also for various biomedical applications..
1. Rie Wakabayashi, Ayato Higuchi, Hiroki Obayashi, Masahiro Goto, Noriho Kamiya, pH-Responsive Self-Assembly of Designer Aromatic Peptide Amphiphiles and Enzymatic Post-Modification of Assembled Structures, International Journal of Molecular Sciences, 10.3390/ijms22073459, 22, 7, 2021.03.
2. Rie Wakabayashi, Hiroki Obayashi, Ryuichiro Hashimoto, Noriho Kamiya, Masahiro Goto, Complementary interaction with peptide amphiphiles guides size-controlled assembly of small molecules for intracellular delivery, Chemical Communications, 10.1039/c9cc02473e, 55, 49, 6997-7000, 2019.06, We introduced complementary interactions between peptide amphiphiles and a small fluorescence dye to develop a programmable multi-component supramolecular assembly, and intracellular delivery of the dye was controlled by the dimensions of the co-assembly, which was manipulated by the peptide design..
3. Rie Wakabayashi, Hidetoshi Kono, Shuto Kozaka, Yoshiro Tahara, Noriho Kamiya, Masahiro Goto, Transcutaneous codelivery of tumor antigen and resiquimod in solid-in-oil nanodispersions promotes antitumor immunity, ACS Biomaterials Science and Engineering, 10.1021/acsbiomaterials.9b00260, 2019.04, Cancer vaccines aim to prevent or inhibit tumor growth by inducing an immune response to tumor-associated antigens (TAAs) encoded by or present in the vaccine. Previous work has demonstrated that effective antitumor immunity can be induced using a codelivery system in which nonspecific immunostimulatory molecules are administered together with TAAs. In this study, we investigated the antitumor effects of a solid-in-oil (S/O) nanodispersion system containing a model TAA, ovalbumin (OVA), and resiquimod (R-848), a small molecular Toll-like receptor 7/8 ligand, which induces an antigen-nonspecific cellular immune response that is crucial for the efficacy of cancer vaccines. R-848 was contained in the outer oil phase of S/O nanodispersion. Analysis of OVA and R-848 permeation in mouse skin after application of an R-848 S/O nanodispersion indicated that R-848 rapidly permeated the skin and preactivated Langerhans cells, resulting in efficient uptake of OVA and migration of antigen-loaded Langerhans cells to the draining lymph nodes. Transcutaneous immunization of mice with an R-848 S/O nanodispersion inhibited the growth of E.G7-OVA tumors and prolonged mouse survival to a greater extent than did immunization with an S/O nanodispersion containing OVA alone. Consistent with this observation, antigen-specific secretion of the Th1 cytokine interferon-γand cytolytic activity were both high in splenocytes isolated from mice immunized with R-848 S/O. Our results thus demonstrate that codelivery of R-848 significantly amplified the antitumor immune response induced by antigen-containing S/O nanodispersions and further suggest that S/O nanodispersions may be effective formulations for codelivery of TAAs and R-848 in transcutaneous cancer vaccines..
4. Rie Wakabayashi, Ayumi Suehiro, Masahiro Goto, Noriho Kamiya, Designer aromatic peptide amphiphiles for self-assembly and enzymatic display of proteins with morphology control, Chemical Communications, 10.1039/C8CC08163H, 55, 5, 640-643, 2019.01.
5. Rie Wakabayashi, Masato Sakuragi, Shuto Kozaka, Yoshiro Tahara, Noriho Kamiya, Masahiro Goto, Solid-in-Oil Peptide Nanocarriers for Transcutaneous Cancer Vaccine Delivery against Melanoma, Mol. Pharm., 10.1021/acs.molpharmaceut.7b00894, 15, 3, 955-961, 2018.03.
6. Rie Wakabayashi, Kensuke Yahiro, Kounosuke Hayashi, Masahiro Goto, Noriho Kamiya, Protein-grafted polymers prepared through a site-specific conjugation by microbial transglutaminase for an immunosorbent assay, Biomacromolecules, 18, 422-430, 2017.02.
7. Yuya Hirakawa, Rie Wakabayashi, Ayaka Naritomi, Masato Sakuragi, Noriho Kamiya, Masahiro Goto, Transcutaneous immunization against cancer using solid-in-oil nanodispersions, Medicinal Chemical Communications, 6, 1387-1392, 2015.07.
8. Rie Wakabayashi, Yuko Abe, Noriho Kamiya, Masahiro Goto, The Self-Assembly and Secondary Structure of Peptide Amphiphiles Determine the Membrane Permeation Activity, The Royal Society of Chemistry, 4, 30654-30657, 2014.07.
9. Rie Wakabayashi, Ryutaro Ishiyama, Noriho Kamiya, Masahiro Goto, A Novel Surface-Coated Nanocarrier for Efficient Encapsulation and Delivery of Camptothecin to Cells, Medicinal Chemical Communications, 5, 1515-1519, 2014.07.
1. Rie Wakabayashi, Enzyme-reactive supramolecular peptide fibers for potential vaccine application, 化学工学会第88年会, 2023.03.
2. Rie Wakabayashi, Bottom-up fabrication of peptide supramolecules and their membrane permeability, Kyushu U-NTNU joint symposium, 2022.11.
3. R. Wakabayashi, A. Higuchi, M. Goto, N. Kamiya, Design of self-assembly peptides for antigenic protein delivery application, The 15th Asian Congress on Biotechnology, 2022.10.
4. R. Wakabayashi, A. Higuchi, M. Goto, N. Kamiya, Self-assembled peptide fibers with enzymatic reactivity for potential delivery of biotherapeutics, APCChE 2022, 2022.08.
5. Rie Wakabayashi, Supramolecular biomaterials based on molecular self-assembly and biochemical engineering, Shinnen Suisan Gakkai FFMS-IPB University 2022 IPB, 2022.07.
6. Hiroki Obayashi, Rie Wakabayashi, Noriho Kamiya, Masahiro Goto, Designer drug delivery carriers constructed through a complementary interaction introduced to self-assembling peptide amphiphiles and a small molecular drug, 18th Asian Pacific Confederation of Chemical Engineering Congress, 2019.09.
7. Rie Wakabayashi, Hiroki Obayashi, Noriho Kamiya, Masahiro Goto, Intracellular delivery of small molecular drugs by complementary interaction with peptide amphiphiles

, The 14th Asian Congress on Biotechnology, 2019.07.
8. R. Wakabayashi, Controlled co-assembly of peptide amphiphiles to form biomimetic nanofibers
, 第28回 日本MRS年次大会, 2018.12.
9. Rie Wakabayashi, Hiroki Obayashi, Noriho Kamiya, Masahiro Goto, Complemantary interaction with peptide amphiphiles guided the intracellular delivery of small molecular drugs, YABEC 2018, 2018.11.
10. Rie Wakabayashi, Controlled assembly of peptide amphiphiles and their application for drug delivery, KIChE-SCEJ joint symposium I, Fall Symposium 2018 of KIChE, 2018.10.
11. R. Wakabayashi, M. Katsuya, N. Kamiya, M. Goto, Multi-block fibrous assembly of peptide amphiphiles based on intrinsic immiscibility, The Second International Symposium on Biofunctional Chemistry (ISBC2017), 2017.12.
Membership in Academic Society
Professional and Outreach Activities
Guidance of experimental class in Kyushu University open campus for high school students..