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Hayashi Nobuya Last modified date:2024.04.08

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Academic Degree
Dr. of Sciences
Country of degree conferring institution (Overseas)
Field of Specialization
plasma application (medicine, agriculture and environment)
Total Priod of education and research career in the foreign country
Outline Activities
Plasma application to engineering:
plasma medicine (Sterilization, Cell activity control)
plasma sterilizer for medicine and food industry
Stimulation of plant growth by plasma irradiation to seeds
Plasma application to environmental issues
Hydrogen production by plasma gasification
Research Interests
  • Development of plasma sterilizer for deep space explorer for planetary protection
    keyword : Plasma sterilization, planetary protection, space probes
  • Interactions between ancient atmospheric components and hydrogen ions
    keyword : formation of sea, CO2, H ion
  • Hydrogen separation from carbonization gas plasma
    keyword : Hydrogen separation, carbonization gas, plasma
  • Modification of molecules on seed coat irradiated by atmospheric oxygen plasma
    keyword : TOF-SIMS, oxygen plasma, seed coat modification
  • Production of Au and Ag nano particles using atmospheric discharge and its bio applications
    keyword : gold nano particles,atmospheric discharge,bio applications
  • Oxidation and removal of dissolved heavy metals by oxygen plasma
    keyword : heavy metal, oxygen plasma, oxidation, removal
  • Control of immune cell activity by oxygen plasma irradiation
    keyword : Oxygen plasma, immune cells, B cells, activity control
  • Decomposition of hard-degradable proteins using oxygen plasma
    keyword : oxygen plsama hard-degradable proteins
  • Activation control of osteoclast and its precursor cells using active species in plasmas
    keyword : plasma, actve species, osteoblast
  • Inactiation of oral cancer cells using active species produced in plasmas
    keyword : plasma, cancer cells, selective inactivation
  • Development of sterilizer using atmospheric plasma for agricultural products
    keyword : atmospheric plasma, UV light, sterilization of agricultural products
  • Plant growth enhancement induced by active oxygen species and its application to agriculture
    keyword : Oxygen plasma, redox reactions, plant, plasma agriculture
  • Development of Medical Sterilizer Using Active Oxygens in Plasma
    keyword : plasma, medical sterilization, active oxygen
Current and Past Project
  • Attempt to sterilize the surface of deep space probe by oxygen plasma.
    Ultimately, we aim to be registered as a space probe sterilization method at COSPAR (International Committee for Space Research).
  • Seeds irradiated with oxygen plasma promote germination and growth.
    In order to elucidate the mechanism of germination and growth promotion, how seeds irradiated with oxygen plasma receive active oxygen and
    The purpose is to clarify whether the active oxygen signal is transmitted to the inside of the seed.
    To analyze the surface of oxygen-plasma-irradiated seeds, TOF-SIMS is used to examine changes in surface molecules.
  • Mass synthesis of silver nanoparticles (Ag NPs) is performed by discharge plasma to generate Ag NPs hydrogel.
    The generated hydrogel is used to sterilize seeds and soil to promote seed germination and growth.
  • By irradiating the seeds of Angelica acutiloba, which is a medicinal plant, with oxygen plasma, germination and growth were promoted, root yield was increased, and medicinal ingredients were increased.
  • To confirm that a chemical-free, zero-emission, high-velocity low-temperature plasma sterilizer that is completely harmless to humans and the environment can be used in spacecraft and confined spaces that are difficult to ventilate, and to determine the optimal operating parameters. Do research. In particular, we clarify the sterilization evaluation and material compatibility when using water plasma. 1. Material suitability: The low-pressure oxygen plasma sterilization method is applied to the sterilization and protein removal of medical equipment and various parts actually used in spacecraft, and the adaptability of this sterilization method to various equipment and parts. reveal gender. 2. Sterilization of the surface of the space probe: Attempt to sterilize the surface of the space probe, and at the same time confirm the suitability of the material. Optimize the water plasma source.
  • Agricultural and medical application research of plasma will be conducted at Kobayashi Laboratory of Chulalongkorn University, Thailand.
  • Sterilize the bacteria attached inside the Itoshima oyster with ozone water.
  • Development of sterilization techniques at various places in food manufacturing cites using plasmas and electric fields.
  • Effect of VUV ozone gas on freshness maintenance and functional improvement of vegetables.
  • Evaluation of medium scale sterilizer and process development based on Japanese Patent No. 4006491 (P 4006491)
Academic Activities
1. Hayashi Nobuya, Yoshinobu Kawai, Industrial Plasma Technology, WILEY VCH, Chapter 2, 2010.10.
2. Hayashi Nobuya, Akikazu Sakudo, Hideharu Shintani, Sterilization and Disinfection by Plasma: Sterilization Mechanisms, Biological and Medical Application, NOVA Publishers, 2010.04.
1. Shameem Ahmed, Sayma Khanom, Nobuya Hayashi, Persistence of Growth Enhancement Induced by Oxygen Plasma Irradiation Seed and Leaf, Agronomy, MDPI,, 13, 6, 1579-1-1579-11, 2023.06, The variation of gene expression of seeds or leaves of Arabidopsis thaliana was investigated by irradiation with oxygen and air plasmas. The irradiation with oxygen plasma reported on the growth promotion and induced the consequence of gene expression in plant cells by neutral active oxygen species. The increase in leaf area ratio by oxygen plasma to seeds was due to epigenetics such as activation of DNA demethylation transcription factors and the growth enhancement effect induced by the plasma irradiation of seeds was inherited by next-generation cells through cell division even after germination. In oxygen irradiation for 10 s, expression of each de-DNA methylation-related gene increased, and DNA methylation-related genes decreased in expression. DNA acetylation that induces gene expressions was suppressed. However, irradiated for 20 s by oxygen, both demethylation suppression and promotion and methylation/acetylation suppression and promotion were obtained. On the other hand, methylation and demethylation may occur at the same time but were not significant and the acetylation was suppressed by air plasma irradiation. In both cases, active oxygen species was the key factor for the variation of gene expression..
2. Reona Muto, Nobuya Hayashi, Sterilization characteristics of narrow tubing by nitrogen oxides generated in atmospheric pressure air plasma, Scientific Reports,, 13, 1, 6947-1-6947-11, 2023.04, The sterilization characteristics of active species generated by an atmospheric dielectric barrier discharge plasma using air and oxygen at the inner surface of silicone tubing were investigated. A dielectric barrier discharge torch plasma device was installed at one end of the tube and generated long-lived active species that flowed into the tube. A strip-type biological indicator with a 105-cell bacterial spore was placed at the opposite end of the 60 cm tube. Sterilization was completed within 30 min by active particles generated from the air plasma. The main factors contributing to the sterilization by air plasma were HNO3 and N2O5. When organic materials (keratin, aspartic acid, and dipicolinic acid) reflecting components of the bacterial spore, were treated by the sterilization procedure there was little effect on dipicolinic acid. Keratin was oxidized by ozone and NOx generated from the oxygen and air plasmas, respectively. Aspartic acid underwent little change in composition from ozone generated from the oxygen plasma, whereas nitro (NO2), nitroso (NO), and aldehyde (CHO) groups were formed from ozone and NOx generated from the air plasma..
3. Nobuya Hayashi, Kyotaro Yamamoto, Variations in Plant Growth Characteristics Due to Oxygen Plasma Irradiation on Leaf and Seed, Agronomy, MDPI,, 12, 2, 259-1-259-19, 2022.02, Gene expression variations of plant leaf are investigated by irradiating seed and leaf with oxygen or air plasmas. Enhancement of leaf growth is induced by oxygen plasma irradiation on seeds, which is supported by increased gene expression for protein synthesis, oxidative-reduction reactions, and decreased gene expression concerning DNA methylation and histone modification. Suppression of leaf growth is observed by the oxygen plasma, which would be owing to increased gene expression concerning heat shock protein and redox reaction, and decreased expression of photosynthesis and glycoprotein. Moreover, gene expression variation due to air plasma irradiation is almost the same as that of oxygen plasma. Active oxygen species are major factors in both oxygen and air plasmas for the variation of gene expressions in plants..
4. K Takaki, K Takahashi, N Hayashi, D Wang, T Ohshima, Pulsed power applications for agriculture and food processing, Reviews of Modern Plasma Physics,, 5, 12, 1-112, 2021.12, Recently, pulsed power technologies, including pulsed electric fields (PEFs) and time-modulated plasmas, are starting to be applied actively in agriculture and food processing. In the applications, compact pulsed power generators with moderate peak power and repetitive operation are developed for controlling discharge plasmas and electric field distribution. These applications are mainly based on the biological effects of a spatially distributed electric field and the chemically active species in the plasma. The PEFs are caused by applying pulse voltage between the electrodes and contribute to form pores on the cell membrane or to change conformation of protein. When the applied voltage exceeds the discharge onset criterion, plasmas are generated through the avalanche process of electron accelerated with intense electric field in a gas or liquids medium. .
5. Sayma Khanom, Nobuya Hayashi, Removal of Metal Ions from Water Using Oxygen Plasmas, Scientific Reports,, 11, 9175, 2021.04, Zinc ion dissolved in water is attempted to be removed by generating the oxides of zinc using the oxygen gas in DBD plasma system. The removal rate of zinc oxides’ production (ZnO and Zn (OH2)) were measured at different treatment periods by the oxygen plasma penetration in water. The removal rate of the deposit increases initially and then decreases with the treatment period. The maximum removal rate (29%) of zinc from water is achieved at the treatment period of 10 min, where pH is minimum. From FTIR the generation properties of zinc oxide can be recognized. Initially the amount the deposit increases with the ozone treatment period due to production of both ZnO and Zn (OH) 2. After that, the production of Zn (OH) 4 2-increases even when the total removal rate of the deposit decreases. Therefore, to remove zinc ion from water forming metal oxide deposit, the penetration amount of the active oxygens to the water must be controlled to keep the pH lower than around 7.5. Because with increasing pH amount of removal rate of ZnO deposit decreases. The pH of the zinc dissolved water treated by ozone depends on both zinc and ozone concentration in water..
6. Sitti Subaedah Sahabuddin, Haruka Ueamatsu, Nobuya Hayashi, Activation of EL-4 T-cells by irradiation with atmospheric oxygen plasma, Japanese Journal of Applied Physics,, 59, SJ, SJJF03, 2020.04, The activation of EL-4 T-cells is investigated when the T-cells are sensitized with monoclonal antibodies against CD3/CD28 followed by irradiation with atmospheric oxygen plasma. The proliferation of anti-CD3/CD28-sensitized EL-4 T-cells was evaluated as a function of the oxygen plasma irradiation duration. The plasma irradiation promotes cell proliferation: when plasma irradiation was applied to sensitize T-cells for 40 s, the cell number increased by five times over 24 h compared with that in a cell population that was not exposed to irradiation. Also, the plasma irradiation increases the production of interferon gamma in T-cells by a factor of two. Gene expression analysis of T-cell supports results of the proliferation and the immune function enhancements..
7. Nobuya Hayashi, Yukie Miyamaru, Reona Aijima, Yoshio Yamashita, Activation of p53-Mediated Apoptosis Pathway in HSC3 Cancer Cell Irradiated by Atmospheric DBD Oxygen Plasma, IEEE Transactions on Plasma Science, 10.1109/TPS.2018.2867431, 47, 2, 1093-1099, 2019.02, Oral cancer cell HSC3 is inactivated by the direct irradiation of oxygen plasma generated by torch-type oxygen dielectric barrier discharge. Selective inactivation of the cancer cells is observed at the discharge voltage of 4.2 kV, where normal cells suffer no damages from the plasma. Inactivation mechanism of HSC3 cells irradiated by the plasma is considered as the apoptosis. Activation of mitogen-activated protein kinase and p53 proteins owing to the phosphorylation is one of the pathways to the cancer cell apoptosis..
8. Hayashi Nobuya, Ono Reoto, Yonesu Akira, Antioxidative activity of plant and regulation of Brassicaceae induced by oxygen radical irradiation, Japanese Journal of Applied Physics, 10.7567/JJAP.54.06GD01, 54, 06GD01-1-06GD01-6, 2015.05.
9. Hayashi Nobuya, Yagyu, Yoshihito, Yonesu Akira, Masaharu Shiratani, Sterilization characteristics of the surfaces of agricultural products using active oxygen species generated by atmospheric plasma and UV light, JAPANESE JOURNAL OF APPLIED PHYSICS, 10.7567/JJAP.53.05FR03, 53, 5, 05FR03-1-05FR03-5, 2014.05.
10. Nobuya Hayashi, Takaomi Nakashima, Akira Yonesu, Sterilization of Medical Equipments Using Air Torch Plasma Produced by Microwave Discharge, IEEE Trans. on Plasma Science, 39, 2976-2977, 2011.12, Sterilization of non-heat-proof equipments was
performed by irradiation from air torch plasma produced by
microwave discharge. The downstream region of the torch
plasma exhibits a yellowish color. Light emission spectra and chemical indicators show that nitrogen oxide radicals are the origin of this color. Inactivation of spore-type Geobacilius stearothermophilus with the population of 105 was successful for a treatment period of 10 minutes..
11. Nobuya Hayashi, Akari Nakahigashi, Masaaki Goto, Satoshi Kitazaki, Kazunori Koga, Masaharu Shiratani, Redox Characteristics of Thiol Compounds Using Radicals Produced by Water Vapor RF Discharge, Jpn. J. Appl. Phys., 50, 08JF04-1 - 08JF04-5, 2011.08.
12. Nobuya Hayashi, Shinsuke Tsutsui, Tetsushi Tomari, Weimin Guan, Sterilization of Medical Equipments Using Oxygen Radicals Produced by Water Vapor RF Plasma, IEEE Trans. on Plasma Science, 36, 1302-1303, 2008.10.
13. Nobuya Hayashi, Weimin Guan, Shinsuke Tsutsui, Tetsushi Tomari, Yasushi Hanada, Sterilization characteristics for medical equipments using radicals produced by oxygen/water vapor RF plasma, Jpn. J. Appl. Phys., 45, 8358-8363, 2006.12.
14. N. Hayashi, Observation of Submicron Dust Particles Trapped in a Diffused Region of a Low Pressure Radio Frequency Plasma, Physics of Plasmas, 8, 6, 3051-3057, 2001.06, Submicron dust particles (Cu, ϕ 50 nm) were observed to be trapped in an rf plasma at relatively low pressure. The laser light scattering by particles shows the time evolution of the dust particles for several hours after pulsed injection of the particles. The dust particles were localized in the diffused region as a large volume cloud. The suspension mechanism of the dust particles in the diffused plasma was explained by an electrostatic force due to the potential structure. The spatial distribution of the charge of the dust particles was determined by this potential structure. The dispersion relations of the self-excited dust acoustic waves were adopted to confirm the spatial distribution and the time evolution of the charge of the dust particles. .
1. Nobuya Haysahi, Changes in seed surface molecular structures due to oxygen plasma irradiation, The 7th International Symposium on Plasma & Fine Bubbles to Agriculture and Aquaculture (7th ISPFB 2023) , 2023.05, In this study, oxygen plasma irradiation to seeds was applied to the cultivation of agricultural products. So far, by irradiating the seeds of typical agricultural products such as carrots, cucumbers, and radishes with oxygen plasma, the effects of growth enhancement and shortening the cultivation time have been obtained. In this experiment, the seeds of Angelica acutiloba, which is a typical medicinal plant but has been difficult to obtain a stable yield, are irradiated with oxygen plasma to enhance germination, growth and medicinal content..
2. 林 信哉、小野大帝、米須 章、田代康介、合島怜於奈, Growth enhancement and gene expression of plants induced by oxygen plasma irradiation, International Toki Conference 2017, 2017.12, 植物種子の遺伝子発現解析とDNAメチル化定量分析を行い、プラズマ照射効果の発現機構としてのエピジェネティクスを確認した。シロイヌナズナの葉面積と全長の増加は、活性酸素種の照射線量に関係する。プラズマ照射された種子から生長した植物の生長速度は、生育期間中の未処理の種子の生育速度よりも速い。シロイヌナズナの種子の遺伝子発現は、酸素プラズマ照射のために数十の遺伝子がアップレギュレートされていることを示している。クロマチン構造およびDNAメチル化などの染色体領域の構造が改変されることが見出され、エネルギー産生系の増強を示す。ヒストンH1.2の減少およびDMEの増加は、ヒストン周辺の遺伝子巻取りおよび遺伝子の脱メチル化をそれぞれ緩やかに誘発する。したがって、遺伝子のエピジェネティックな改変により、酸素プラズマの照射効果の継承が起こることが明らかとなった。.
3. 林 信哉、三根圭介、宮丸由紀恵、合島怜於奈、山下佳雄、後藤昌昭, Regulation of activity and differentiation of animal cells using active oxygen species in atmospheric plasma, Asia-Pacific International Symposium on the Basics and Applications of Plasma Technology (APSPT-10), 2017.12, プラズマを用いた細胞不活性化技術は、がんや骨粗鬆症の治療に有効であると期待されている。 しかし、細胞へのプラズマ照射の影響とその不活性化メカニズムは依然として不明であり、特にがん細胞と正常細胞との選択性の機構解明がなされておらず,実用化の障壁となっている。 本研究では、DBDプラズマによって生成された活性酸素種の口腔がん細胞および正常細胞に対する不活性化効果を調べた。.
4. 林 信哉,中野 陸,小野大帝,田代康介,久原 哲,合島怜於奈, Modification of gene expression of plants induced by active oxygen species in oxygen plasma, IUMRS-ICAM 2017, 2017.09, 遺伝子発現解析とDNAメチル化定量解析を行い、エピジェネティクスがプラズマ照射効果の遺伝機構であることを確認した。 ヒストンH1.2の減少およびDMEの増加から、ヒストンの周りの遺伝子巻取りの緩和および遺伝子の脱メチル化が明らかとなった。 DNA脱メチル化が緩慢であることから、遺伝子発現の増加が示唆された。 また、DNAメチル化量とプラズマ中の励起酸素量が酸素ガス圧力に関して同様の傾向を有することが分かった。 以上の結果から、活性酸素種の照射により遺伝子のエピジェネティックな改変が生じ、増殖促進効果の遺伝機構がエピジェネティクスに起因すると結論づけられた。.
5. Tetsuya Ohtsubo, Nobuya Hayashi, Effect of active species on animal cells in culture media induced by DBD Plasma irradiation using air, 68th Annual Gaseous Electronics Conference held jointly with 9th Annual International Conference Plasma & 33rd Symposium on Plasma Processing, 2015.10.
6. Yasuhiro Sakai, Nobuya Hayashi, Sterilization Performance and Material Compatibility of Sterilizer for Dental Instruments using RF Oxygen Plasma, 68th Annual Gaseous Electronics Conference held jointly with 9th Annual International Conference Plasma & 33rd Symposium on Plasma Processing, 2015.10.
7. Nobuya Hayashi, Masaharu Shiratani, Sterilization of Agricultural Products Using Oxygen Radicals Produced by Barrier Discharge, 8th International Conference on Reactive Plasmas and 31st Symposium on Plasma Processing, 2014.02.
Membership in Academic Society
  • Sigma Xi (The Scientific Research Honor Society)
  • The Japan Society of Applied Physics
  • The Japan Society of Plasma Science and Nuclear Fusion Research
  • Institute of Applied Plasma Science
  • Society of Geomagnetism and Earth, Planetary and Space Sciences
  • The Japanese Society of Oral and Maxillofacial Surgeons
  • Atmospheric pressure helium torch plasma was used to efficiently induce apoptosis in oral cancer cells, contributing to the practical application of plasma-based cancer treatment.
  • By irradiating oral cancer cells with atmospheric pressure helium plasma, we succeeded in capturing the induction of apoptosis as a time-lapse movie.
  • By using atmospheric pressure air plasma, we succeeded for the first time in the world in sterilizing the inner surface of a catheter of about 1 m, which had not been realized so far.
Educational Activities
Lecture on plasma fundamental physics for graduate students.

Plasma application research using plasma generation equipment for graduate students (medical / bio application, agricultural application).

Lecture and experiment on electromagnetic for undergraduate students.

Plasma application research using plasma generation equipment for undergraduate students (medical / bio application, agricultural application).