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Akihiro Kishimura Last modified date：2023.09.12

Associate Professor / Department of Applied Chemistry
Faculty of Engineering


Graduate School
Department of Systems Life Sciences
Graduate School of Systems Life Sciences
Undergraduate School
Department of Materials Science and Engineering
School of Engineering
Other Organization
Other
Integrated Initiative for Designing Future Society
Center for Future Chemistry
Office for Promoting Cooperation with Society
Center for Molecular Systems (CMS)
Administration Post
Executive Adviser to the President


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Homepage
https://kyushu-u.pure.elsevier.com/en/persons/akihiro-kishimura
　Reseacher Profiling Tool　Kyushu University Pure
https://www.researchgate.net/profile/Akihiro-Kishimura
ResearchGate .
https://researchmap.jp/read0103082?lang=en
Reserachmap (English) .
http://www.chem.kyushu-u.ac.jp/~cms/english/
The web page of the center for molecular systems. .
https://sites.google.com/view/katayamalab
Katayama Lab's HP. .
Phone
092-802-2851
Fax
092-802-2851
Academic Degree
Ph. D.
Country of degree conferring institution (Overseas)
No
Field of Specialization
Polymer Chemistry, Supramolecular Chemistry, Biomaterials, Drug Dleivery System
ORCID(Open Researcher and Contributor ID)
0000-0002-0503-1418
Total Priod of education and research career in the foreign country
00years02months
Research
Research Interests
  • Rational construction of hierarchical structures based on phase separation from transient condensates of biomacromolecules
    keyword : Vesicles, Coacervates, Hierachical Structures, Artificial Organellas, Biomacromolecules
    2022.04～2022.06.
  • Development of polymeric yolk-shell structure as a protein-concentration capsule and its application for efficient cascade reaction
    keyword : enzyme, proteins, polyion complexes, yolk-shell structure, condensed environment, liquid-liquid phase separation, cascade reactions
    2020.06～2020.03.
  • Establishment of liquid-liquid phase separation model and understanding of function of crowded environments toward artificial cytosol design
    keyword : coacervate, liquid-liquid phase separation, proteins
    2019.06～2021.03.
  • TBA
    keyword : bioluminescence, nanomedicine, vesicles
    2017.04～2019.07.
  • Establishment of soft material platform based on nano-structured coacervates
    keyword : coacervate, block copolymers, polyion complexes, hybrid materials, proteins
    2015.04.
  • TBA
    keyword : Drug Delivery System, Nanomedicine, pharmacokinetics, nano-physiology
    2018.04～2022.03.
  • Develpment of novel enzyme-loaded polymeric nano-capsules desinged for in vivo applications
    keyword : enzyme, nanoreactors, protein delivery system, nano-medicine, vesicles
    2014.04～2017.03.
  • Development of novel nanoreactor system utilizing molecularly crowded nano-compartments
    keyword : Nano-compartment, Vesicles, Molecular Crowding, Enzymatic Reaction
    2013.04～2015.03.
Academic Activities
Reports
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1. An emerging material “PICsome”: A hot zone between “PEG” and “PEG”

Recently, nanomaterials constructed by molecular self-assembly have gathered much attention to develop nano-devices incorporated with many types of drugs. Particularly, hollow capsules are one of promising materials, and recently, we have developed polyion complex vesicles, PICsomes, as novel polymeric vesicles. The most advantageous feature of PICsomes is its simple preparation process: Typically, they can be prepared by simple mixing of oppositely charged block copolymers consisting of poly(ethylene glycol) (PEG) and charged poly(amino acid)s in an aqueous medium. Moreover, many other unique properties of PICsomes have been reported, such as facile tuning of vesicle sizes ranging from 100–400 nm while keeping monodispersed size distribution, semipermeable vesicle membrane, facile loading of various water-dispersed materials, long blood circulation after crosslinking, excellent tumor accumulation based on the enhanced permeability and retention (EPR) effect, and so on. The present review article describes basic design and synthetic strategy of PICsomes, fundamental properties of PICsomes, and recent applications of PICsomes to drug delivery system..
2. Akihiro Kishimura, Horacio Cabral, Kanjiro MIyata, Nanodevices for studying nano-pathophysiology, Advanced Drug Delivery reviews, DOI: 10.1016/j.addr.2014.06.003, 2014.06, Nano-scaled devices are a promising platform for specific detection of pathological targets, facilitating the anal- ysis of biological tissues in real-time, while improving the diagnostic approaches and the efficacy of therapies. Herein, we review nanodevice approaches, including liposomes, nanoparticles and polymeric nanoassemblies, such as polymeric micelles and vesicles, which can precisely control their structure and functions for specifically interacting with cells and tissues. These systems have been successfully used for the selective delivery of reporter and therapeutic agents to specific tissues with controlled cellular and subcellular targeting of biomolecules and programmed operation inside the body, suggesting a high potential for developing the analysis for nano- pathophysiology..
3. Akihiro Kishimura, Development of polyion complex vesicles (PICsomes) from block copolymers for biomedical applications, Polymer Journal, 2013.04, [URL], Polyion complex (PIC) formation is one of the most powerful techniques for obtaining molecular self-assemblies in aqueous media. The simple preparation process based on multiple electrostatic interactions is quite attractive for material syntheses, as well as biomedical applications. Therefore, it is desirable to control PIC architectures at the nanoscale in order to expand the scope of PIC materials. In this review article, recent progress on PIC vesicles (PICsomes) is summarized. PICsomes were first developed by my research group, and we recently succeeded in controlling the sizes and structural uniformity of the vesicles. Furthermore, the characteristic dynamic nature of PICs was revealed: PICs were found to exhibit reversible association/ dissociation and structural transformation. We demonstrated that crosslinking the PIC layers of PICsomes is a powerful method for tuning properties such as stability and permeability. Finally, the potential utility of PICsomes for drug delivery nanocarriers was examined, and their future biomedical application is discussed..
4. Development and Biomedical Applications of Polymeric Hollow Capsules “PICsomes” Possessing Semipermeable Polyion Complex Membrane.
Papers
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1. Biplab K C, Teruki Nii, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Dynamic frustrated charge hotspots created by charge density modulation sequester globular proteins into complex coacervates, Chemical Science, 10.1039/d3sc00993a, 14, 24, 6608-6620, 2023.05, This study presents a simple strategy for the sequestration of globular proteins as clients into synthetic polypeptide-based complex coacervates as a scaffold, thereby recapitulating the scaffold-client interaction found in biological condensates. Considering the low net charges of scaffold proteins participating in biological condensates, the linear charge density (s) on the polyanion, polyethylene glycol-b-poly(aspartic acids), was reduced by introducing hydroxypropyl or butyl moieties as a charge- neutral pendant group. Complex coacervate prepared from the series of reduced-s polyanions and the polycation, homo-poly-L-lysine, could act as a scaffold that sequestered various globular proteins with high encapsulation efficiency (>80%), which sometimes involved further agglomerations in the coacervates. The sequestration of proteins was basically driven by electrostatic interaction, and therefore depended on the ionic strength and charges of the proteins. However, based on the results of polymer partitioning in the coacervate in the presence or absence of proteins, charge ratios between cationic and anionic polymers were maintained at the charge ratio of unity. Therefore, the origin of the electrostatic interaction with proteins is considered to be dynamic frustrated charges in the complex coacervates created by non-neutralized charges on polymer chains. Furthermore, fluorescence recovery after photobleaching (FRAP) measurements showed that the interaction of side-chains and proteins changed the dynamic property of coacervates. It also suggested that the physical properties of the condensate are tunable before and after the sequestration of globular proteins. The present rational design approach of the scaffold-client interaction is helpful for basic life-science research and the applied frontier of artificial organelles..
2. Akinori Goto, Yasutaka Anraku, Shigeto Fukushima and Akihiro Kishimura, Increased Enzyme Loading in PICsomes Via Controlling Membrane Permeability Improves Enzyme Prodrug Cancer Therapy Outcome, Polymers, https://doi.org/10.3390/polym15061368, 15, 6, 1368, 2023.03.
3. Asmariah Ahmad, Tomoki Maruyama, Teruki Nii, Takeshi Mori, Yoshiki Katayama, and Akihiro Kishimura, Facile preparation of hexagonal nanosheets via polyion complex formation from α-helical polypeptides and polyphosphate-based molecules, Chemical Communications, 10.1039/d2cc05137k, 59, 12, 1657-1660, 2023.01.
4. Asmariah Ahmad, Teruki Nii, Takeshi Mori, Yoshiki Katayama, Masanori Toyofuku, Akihiro Kishimura, Nanostructure Control of an Antibiotic-Based Polyion Complex Using a Series of Polycations with Different Side-Chain Modification Rates., Macromolecular rapid communications, 10.1002/marc.202200316, e2200316, 2022.06, Developing nanovehicles for delivering antibiotics is a promising approach to overcome the issue of antibiotic resistance. This study aims to utilize a polyion complex (PICs) system for developing novel nanovehicles for polymyxin-type antibiotics, which are known as last resort drugs. The formation of antibiotic-based PIC nanostructures is investigated using colistimethate sodium (CMS), an anionic cyclic short peptide, and a series of block catiomers bearing different amounts of guanidinium moieties on their side chains. In addition, only the modified catiomer, and not the unmodified catiomer, self-assembles with CMS, implying the importance of the guanidine moieties for enhancing the interaction between the catiomer and CMS via the formation of multivalent hydrogen bonding. Moreover, micellar and vesicular PIC nanostructures are selectively formed depending on the ratio of the guanidine residues. Size-exclusion chromatography reveals that the encapsulation efficiency of CMS is dependent on the guanidinium modification ratio. The antimicrobial activity of the PIC nanostructures is also confirmed, indicating that the complexation of CMS in the PICs and further release from the PICs successfully occurs..
5. Yiwei Liu, Tomoki Maruyama, K. C. Biplab, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Inducible Dynamic Behavior of Polyion Complex Vesicles by Disrupting Charge Balance, CHEMISTRY LETTERS, 10.1246/cl.210037, 50, 5, 1034-1037, 2021.05, The formation and dynamic behavior of polyion complex-based vesicles (PICsomes) were examined under charge-balance disrupting conditions, using homo-catiomer and pegylated-aniomer. Under catiomer-rich conditions, an unusual increase in the PICsome size was observed, which was primarily attributed to Ostwald ripening. This growth was triggered by the addition of a homo-catiomer to switch the composition of the PIC membrane. The results provide insight into the design of smart PIC-based systems for functional nanocapsules..
6. Beob Soo Kim, Mitsuru Naito, Hiroyuki Chaya, Mao Hori, Kotaro Hayashi, Hyun Su Min, Yu Yi, Hyun Jin Kim, Tetsuya Nagata, Yasutaka Anraku, Akihiro Kishimura, Kazunori Kataoka, and Kanjiro Miyata, Noncovalent Stabilization of Vesicular Polyion Complexes with Chemically Modified/Single-Stranded Oligonucleotides and PEG-b-guanidinylated Polypeptides for Intracavity Encapsulation of Effector Enzymes Aimed at Cooperative Gene Knockdown, Biomacromolecules, 10.1021/acs.biomac.0c01192, 21, 10, 4365-4376, 2020.09, For the simultaneous delivery of antisense oligonucleotides and their effector enzymes into cells, nanosized vesicular polyion complexes (PICs) were fabricated from oppositely charged polyion pairs of oligonucleotides and poly(ethylene glycol) (PEG)-b-polypeptides. First, the polyion component structures were carefully designed to facilitate a multimolecular (or secondary) association of unit PICs for noncovalent (or chemical cross-linking-free) stabilization of vesicular PICs. Chemically modified, single-stranded oligonucleotides (SSOs) dramatically stabilized the multimolecular associates under physiological conditions, compared to control SSOs without chemical modifications and duplex oligonucleotides. In addition, a high degree of guanidino groups in the polypeptide segment was also crucial for the high stability of multimolecular associates. Dynamic light scattering and transmission electron microscopy revealed the stabilized multimolecular associates to have a 100 nm sized vesicular architecture with a narrow size distribution. The loading number of SSOs per nanovesicle was determined to be ∼2500 using fluorescence correlation spectroscopic analyses with fluorescently labeled SSOs. Furthermore, the nanovesicle stably encapsulated ribonuclease H (RNase H) as an effector enzyme at ∼10 per nanovesicle through simple vortex-mixing with preformed nanovesicles. Ultimately, the RNase H-encapsulated nanovesicle efficiently delivered SSOs with RNase H into cultured cancer cells, thereby eliciting the significantly higher gene knockdown compared with empty nanovesicles (without RNase H) or a mixture of nanovesicles with RNase H without encapsulation. These results demonstrate the great potential of noncovalently stabilized nanovesicles for the codelivery of two varying bio-macromolecule payloads for ensuring their cooperative biological activity..
7. Beob Soo Kim, Mitsuru Naito, Rimpei Kamegawa, Hyun Jin Kim, Ryo Iizuka, Takashi Funatsu, Shingo Ueno, Takanori Ichiki, Akihiro Kishimura and Kanjiro Miyata, Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown, CHEMICAL COMMUNICATIONS, 10.1039/d0cc01750g, 56, 66, 9477-9480, 2020.08.
8. Omer F. Mutaf, Yasutaka Anraku, Akihiro Kishimura, Kazunori Kataoka, Unilamellar polyion complex vesicles (PICsomes) with tunable permeabilities for macromolecular solutes with different shapes and sizes, POLYMER, 10.1016/j.polymer.2017.10.062, 133, 1-7, 2017.12, Polyion complex vesicles (PICsomes) are characterized by their unique three-layered semipermeable nanomembrane structures, in which a unilamellar PIC layer is sandwiched by poly(ethylene glycol) layers, and have gathered much attention as nano-scaled drug vehicles. Herein, the crosslinking degree of the nanomembrane in the PICsome was controlled systematically for the first time. Permeability of the PICsome nanomembrane was evaluated through a kinetic study of the release of macromolecular cargoes from the PICsome. The degree of crosslinking in the nanomembrane successfully regulated the release behavior. Moreover, the shape and size of the macromolecular solutes were found to be critical factors determining their transport from the inner aqueous phase of the PICsome to the external environment. The results indicate that the unique three-layered structure of PICsome membranes plays a key role in modulating solute transport. These findings will provide a rational strategy for the development of nanomembrane-based controlled-release systems. (C) 2017 Elsevier Ltd. All rights reserved..
9. Akinori Goto, Hui Chi Yen, Yasutaka Anraku, Shigeto Fukushima, Ping-Shan Lai, Masaru Kato, Akihiro Kishimura, Kazunori Kataoka, Facile Preparation of Delivery Platform of Water-Soluble Low-Molecular-Weight Drugs Based on Polyion Complex Vesicle (PlCsome) Encapsulating Mesoporous Silica Nanoparticle, ACS BIOMATERIALS SCIENCE & ENGINEERING, 10.1021/acsbiomaterials.6b00562, 3, 5, 807-815, 2017.05, Polyion complex vesicles (PlCsomes) are polymeric hollow capsules composed of a unique semipermeable membrane, which may represent a versatile platform for constructing drug-loaded nanoformulation. However, it is difficult to retain water-soluble low-molecular weight compounds (LMWCs) in the inner space of PlCsome because of the high permeability of PIC membrane for LMWCs. Herein, we selected mesoporous silica nanoparticle (MSN) as a drug-retaining nanomatrix, and we demonstrated successful encapsulation of MSN into the PlCsome to obtain MSN@PlCsome. The efficacy of MSN loading, a ratio of the amount of MSN encapsulated in the PICsome to the amount of feed MSN, was at most 83%, and the diameter of resulting product was approximately 100 nm. The obtained MSN@PlCsome was stably dispersed under the physiological condition, and showed considerable longevity in blood circulation of mice. Furthermore, the surface of MSN in MSN@PlCsome can be modified without any deterioration of the vesicle structure, obtaining amino-functionalized and sulfonate-functionalized MSN@PlCsomes (A-MSN@PlCsome and S-MSN@PICsome, respectively). Both surface-modified MSN@PlCsomes were successfully loaded with charged water-soluble low-molecular-weight compounds (LMWCs). Particularly, S-MSN@PICsome kept 8 wt % gemcitabine (GEM) per S-MSN, and released it in a sustained manner. GEM-loaded S-MSN@PlCsome demonstrated marked cytotoxicity against cultured tumor cells, and achieved significant in vivo efficacy to suppress the growth of subcutaneously implanted lung tumor via intravenous administration..
10. Kenshiro Naoyama, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Fabrication of Dendrimer-Based Polyion Complex Submicrometer-Scaled Structures with Enhanced Stability under Physiological Conditions, MACROMOLECULAR RAPID COMMUNICATIONS, 10.1002/marc.201600171, 37, 13, 1087-1093, 2016.07, Submicrometer-scaled (sub-) self-assembled materials have been developed based on polyion complex (PIC) formation, in particular for biomedical-applications. However, sufficient stability under physiological conditions is required for their practical use. In this study, PIC formation behavior is examined using a block aniomer, poly(ethylene glycol)-b-poly(aspartic acid), and homocatiomers, poly(l-lysine) (LPK) and dendritic poly(l-lysine) (DPK) with different generations, to elucidate the contribution of the dendritic architecture to stability enhancement. LPK-based PIC shows a sub-vesicular structure only at 25 degrees C in the absence of NaCl; in contrast, DPK-based PIC forms a sub-structure under physiological salt concentration and temperature conditions, even when the number of charges of a single molecule is much smaller than that of LPK. Moreover, the formation of sub-vesicular and -spherical micellar structures is dependent on DPK generation. Thus, the molecular backbone architecture of the PIC component plays an important role not only in expanding the preparation conditions and enhancing stability, but also in controlling the self-assembled structures, mainly due to the spatially restricted structures of dendrimers..
11. Ryosuke Taniguchi, Yutaka Miura, Hiroyuki Koyama, Tsukasa Chida, Yasutaka Anraku, Akihiro Kishimura, Kunihiro Shigematsu, Kazunori Kataoka, Toshiaki Watanabe, Adequately-Sized Nanocarriers Allow Sustained Targeted Drug Delivery to Neointimal Lesions in Rat Arteries, MOLECULAR PHARMACEUTICS, 10.1021/acs.molpharmaceut.6b00219, 13, 6, 2108-2116, 2016.06, In atherosclerotic lesions, the endothelial barrier against the bloodstream can become compromised, resulting in the exposure of the extracellular matrix (ECM) and intimal cells beneath. In theory, this allows adequately sized nanocarriers in circulation to infiltrate into the intimal lesion intravascularly. We sought to evaluate this possibility using rat carotid arteries with induced neointima. Cy5-labeled polyethylene glycol-conjugated polyion complex (PIC) micelles and vesicles, with diameters of 40, 100, or 200 nm (PICs-40, PICs-100, and PICs-200, respectively) were intravenously administered to rats after injury to the carotid artery using a balloon catheter. High accumulation and long retention of PICs-40 in the induced neointima was confirmed by in vivo imaging, while the accumulation of PICs-100 and PICs-200 was limited, indicating that the size of nanocarriers is a crucial factor for efficient delivery. Furthermore, epirubicin-incorporated polymeric micelles with a diameter similar to that of PICs-40 showed significant curative effects in rats with induced neointima, in terms of lesion size and cell number. Specific and effective drug delivery to pre-existing neointimal lesions was demonstrated with adequate size control of the nanocarriers. We consider that this nanocarrier-based drug delivery system could be utilized for the treatment of atherosclerosis..
12. Yasutaka Anraku, Akihiro Kishimura, Mako Kamiya, Sayaka Tanaka, Takahiro Nomoto, Kazuko Toh, Yu Matsumoto, Shigeto Fukushima, Daiki Sueyoshi, Mitsunobu R. Kano, Yasuteru Urano, Nobuhiro Nishiyama, Kazunori Kataoka, Systemically Injectable Enzyme-Loaded Polyion Complex Vesicles as In Vivo Nanoreactors Functioning in Tumors, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 10.1002/anie.201508339, 55, 2, 560-565, 2016.01, The design and construction of nanoreactors are important for biomedical applications of enzymes, but lipid-and polymeric-vesicle-based nanoreactors have some practical limitations. We have succeeded in preparing enzyme-loaded polyion complex vesicles (PICsomes) through a facile protein-loading method. The preservation of enzyme activity was confirmed even after cross-linking of the PICsomes. The crosslinked beta-galactosidase-loaded PICsomes (beta-gal@PICsomes) selectively accumulated in the tumor tissue of mice. Moreover, a model prodrug, HMDER-beta Gal, was successfully converted into a highly fluorescent product, HMDER, at the tumor site, even 4 days after administration of the beta-gal@PICsomes. Intravital confocal microscopy showed continuous production of HMDER and its distribution throughout the tumor tissues. Thus, enzyme-loaded PICsomes are useful for prodrug activation at the tumor site and could be a versatile platform for enzyme delivery in enzyme prodrug therapy..
13. Akihiro Kishimura, An emerging material “PICsome”
A hot zone between “PEG” and “PEG”, Drug Delivery System, 10.2745/dds.31.308, 31, 4, 308-319, 2016.01, Recently, nanomaterials constructed by molecular self-assembly have gathered much attention to develop nano-devices incorporated with many types of drugs. Particularly, hollow capsules are one of promising materials, and recently, we have developed polyion complex vesicles, PICsomes, as novel polymeric vesicles. The most advantageous feature of PICsomes is its simple preparation process: Typically, they can be prepared by simple mixing of oppositely charged block copolymers consisting of poly(ethylene glycol) (PEG) and charged poly(amino acid)s in an aqueous medium. Moreover, many other unique properties of PICsomes have been reported, such as facile tuning of vesicle sizes ranging from 100-400 nm while keeping monodispersed size distribution, semipermeable vesicle membrane, facile loading of various water-dispersed materials, long blood circulation after crosslinking, excellent tumor accumulation based on the enhanced permeability and retention (EPR) effect, and so on. The present review article describes basic design and synthetic strategy of PICsomes, fundamental properties of PICsomes, and recent applications of PICsomes to drug delivery system..
14. Omer F. Mutaf, Akihiro Kishimura, Yuki Mochida, Ahram Kim, Kazunori Kataoka, Induction of Secondary Structure through Micellization of an Oppositely Charged Pair of Homochiral Block-and Homopolypeptides in an Aqueous Medium, MACROMOLECULAR RAPID COMMUNICATIONS, 10.1002/marc.201500368, 36, 22, 1958-1964, 2015.11, Polyion complex (PIC) formation is an attractive method for obtaining molecular assemblies owing to their facile fabrication process in aqueous media, but more insights are required in order to control the higher-dimensional structures of polypeptide-based PICs. Herein, the PIC formation behavior of oppositely charged homochiral polypeptides, poly-L-lysine and poly(ethylene glycol)-b-poly(L-glutamate) (PEG-PLG), and their secondary structures are carefully studied in water. PIC formation takes place in a polymer concentration-dependent manner, and clear beta-sheet formation is observed at polymer concentrations >= 0.3 mg mL(-1). The results also confirm that multimolecular aggregation is a prerequisite for beta-sheet formation, which indicates that the inner hydrophobic environment of PICs is favorable for beta-sheet formation. Furthermore, the PEG weight fraction, stereoregularity of the polypeptide, and ionic strength of the solutions are found to be key factors for generating a secondary structure, presumably because these factors can contribute to the tuning of the inner environment of PICs. This method of producing water-soluble nanoassemblies from oppositely charged polypeptides may expedite self-assembly studies in biological systems and be incorporated into various molecular systems to exploit protein-mimicking features..
15. D. Sueyoshi, A. Kishimura, H. Oana, Y. Anraku, M. Takai, M. Washizu, K. Kataoka, Microchannel-assisted preparation of polyion complex vesicles and real-time observation of their dynamic responses to external electric fields, MicroTAS, 3, 1882-1884, 2014.08.
16. Development of SPIO-loaded Unilamellar Polyion Complex Vesicles (SPIO-Cy5-PICsomes) as a High Relaxivity Contrast Agent for Early-stage Tumor Detection.
17. Sayan Chuanoi, Yasutaka Anraku, Mao Hori, Akihiro Kishimura, Kazunori Kataoka, Fabrication of Polyion Complex Vesicles with Enhanced Salt and Temperature Resistance and Their Potential Applications as Enzymatic Nanoreactors, BIOMACROMOLECULES, 10.1021/bm500127g, 15, 7, 2389-2397, 2014, 2014.07, Integrating catalytic functions into polymeric vesicles through enzyme entrapment is appealing for bioreactor fabrication, yet there are critical issues regarding the regulation of solute transport through membranes and enzyme loading without denaturation. Polyion complex vesicles (PICsomes) with semipermeable membranes and the propensity to form in water can overcome these issues; however, cross-linking is required for sufficient physiological stability. Herein, we report the first successful fabrication of non-cross-linked PICsomes with sufficient stability at physiological salinity and temperature by tuning the hydrophobicity of the aliphatic side chains in the pendant group of the constituent polyelectrolytes. Dynamic light scattering and transmission electron microscopy revealed that the intervesicular fusion and disintegration of the PICsomes was prevented and a narrow distribution was maintained at physiological salinity and temperatures. Furthermore, their application as enzymatic nanoreactors was verified even in the presence of proteases. As such, the potential utility of the PICsomes in biomedical fields was established..
18. Horacio Cabral, Kanjiro Miyata, Akihiro Kishimura, Nanodevices for studying nano-pathophysiology, ADVANCED DRUG DELIVERY REVIEWS, 10.1016/j.addr.2014.06.003, 74, 35-52, 2014.07, Nano-scaled devices are a promising platform for specific detection of pathological targets, facilitating the analysis of biological tissues in real-time, while improving the diagnostic approaches and the efficacy of therapies. Herein, we review nanodevice approaches, including liposomes, nanoparticles and polymeric nanoassemblies, such as polymeric micelles and vesicles, which can precisely control their structure and functions for specifically interacting with cells and tissues. These systems have been successfully used for the selective delivery of reporter and therapeutic agents to specific tissues with controlled cellular and subcellular targeting of biomolecules and programmed operation inside the body, suggesting a high potential for developing the analysis for nano-pathophysiology. (C) 2014 Elsevier B.V. All rights reserved..
19. Arie Wibowo, Kensuke Osada, Hiroyuki Matsuda, Yasutaka Anraku, Haruko Hirose, Akihiro Kishimura, Kazunori Kataoka, Morphology Control in Water of Polyion Complex Nanoarchitectures of Double-Hydrophilic Charged Block Copolymers through Composition Tuning and Thermal Treatment, MACROMOLECULES, 10.1021/ma500314d, 47, 9, 3086-3092, 2014.05, Polyion complexes (PICs) are attractive as eco-friendly materials, because they offer simple and fast preparation methods to exert various functionalities in aqueous medium. However, control of nanoarchitectures in PIC materials has not been fully realized, except for the case of micelles and unilamellar vesicles formed from block ionomers. Here, the procedure to control PIC nanoarchitectures with various morphologies was established for the first time by careful tuning in the composition of PICs made from PEG-based block-ionomers with a varying amount of homoionomers as additive to modulate the PEG weight fraction (f(PEG)) in the obtained PICs. Accordingly, the variation in f(PEG) from 12.1% to 6.5% induced vigorous transition in the microphase separated structures of PICs basically from micelle to lamella via cylindrical network. Notably, uniformed lamella with alternative layers of PEG and PIG domains was found at elevated temperature (70 degrees C), which, by lowering temperature, reversibly transformed to cylindrical P1C network apparently with connected aqueous channel in mesoscopic scale..
20. Akihiro Kishimura, Morphology Control in Water of Polyion Complex Nanoarchitectures of Double-Hydrophilic Charged Block Copolymers through Composition Tuning and Thermal Treatment. , American Chemical Society, 10.1021/ma500314d , 47, 9, 3086-3092, 2014, 2014.04.
21. Daisuke Kokuryo, Yasutaka Anraku, Akihiro Kishimura, Sayaka Tanaka, Mitsunobu R. Kano, Jeff Kershaw, Nobuhiro Nishiyama, Tsuneo Saga, Ichio Aoki, Kazunori Kataoka, SPIO-PICsome: Development of a highly sensitive and stealth-capable MRI nano-agent for tumor detection using SPIO-loaded unilamellar polyion complex vesicles (PICsomes) (vol 169, pg 220, 2013), JOURNAL OF CONTROLLED RELEASE, 10.1016/j.jconrel.2014.01.021, 178, 125-125, 2014.03.
22. Sayan Chuanoi, Akihiro Kishimura, Wen-Fei Dong, Yasutaka Anraku, Yuichi Yamasaki, Kazunori Kataoka, Structural factors directing nanosized polyion complex vesicles (Nano-PICsomes) to form a pair of block aniomer/homo catiomers: studies on the aniomer segment length and the catiomer side-chain structure, POLYMER JOURNAL, 10.1038/pj.2013.82, 46, 2, 130-135, 2014.02, Much attention has been devoted to precise control of the size and morphology in nanosized molecular assemblies for a wide range of materials applications. Recently, we reported observing submicron/nanosized polyion complex vesicles (Nano-PICsomes) with a narrow size distribution, synthesized using specific types of homocatiomers and polyethylene glycol (PEG)-based block aniomers. However, only one example of Nano-PICsomes has been reported to date. Here, the role of the chemical composition of PEG-based block aniomers and the chemical structures of the side chains of homocatiomers were carefully examined to better understand the formation of Nano-PICsomes. Transmission electron microscopy and dynamic light scattering analyses of Nano-PICsomes revealed that a longer length of ionic segments in the block aniomers or a PEG weight fraction (f(PEG)) 10% produced spherical micelles. In addition, the homocatiomers containing longer aliphatic side chains (e. g., five or six carbon atoms) favored the formation of Nano-PICsomes, whereas those containing shorter aliphatic side chains produced irregularly shaped PIC micelles. Accordingly, f(PEG) and the length of the side chain were found to be the key factors that control the morphologies of Nano-PICsomes. Insights gained from this study can broaden the spectrum of the design of Nano-PICsomes for use in a diverse range of material applications..
23. Huabing Chen, Ling Xiao, Yasutaka Anraku, Peng Mi, Xueying Liu, Horacio Cabral, Aki Inoue, Takahiro Nomoto, Akihiro Kishimura, Nobuhiro Nishiyama, Kazunori Kataoka, Polyion Complex Vesicles for Photoinduced Intracellular Delivery of Amphiphilic Photosensitizer, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 10.1021/ja406992w, 136, 1, 157-163, 2014, 2014.01, Polymer vesicles formed by a pair of oppositely charged poly(ethylene glycol) (PEG)-based block aniomer and homocatiomer, termed "PICsomes", have tunable size, and are characterized by unique semipermeable property due to the flexible and tunable hydrophilicity of polyion complex (PIC) membranes. The PICsomes can encapsulate a variety of molecules in an inner aqueous phase just by a simple vortex mixing of solution, expecting their utility as nanocontainers of substances with biomedical interests. Here, we report on a new functionality of the PICsomes: photoinduced release of photoactive agents for intracellular drug delivery. A potent photosensitizer, Al(III) phthalocyanine chloride disulfonic acid (AlPcS2a), was efficiently incorporated into the PICsomes (11%(w/w)), and its quick release was induced by photoirracliation possibly due to the photochemical damage of the PIG membranes. The combination of a high-resolution fluorescent confocal microscopy and a lysosome membrane-specific staining method revealed that such photoinduced release of AlPcS2a occurred even in the lysosomes of living cells after endocytic internalization. Simultaneously, the released AlPcS2a photochemically affected the integrity of the lysosomal membranes, leading to the translocation of AlPcS2a and PICsomes themselves to the cytoplasm. Consequently, the AlPcS2a-encapsulated PICsomes (AIPcS2a-PICsomes) exhibited appreciably stronger photocytotoxicity compared with free AlPcS2a alone. Thus, the AlPcS2a-PICsomes have promising feasibility for the photodynamic therapy or the photoinduced cytoplasmic delivery of therapeutic molecules..
24. Akihiro Kishimura, Development of polyion complex vesicles (PICsomes) from block copolymers for biomedical applications, POLYMER JOURNAL, 10.1038/pj.2013.33, 45, 9, 892-897, 2013.09, Polyion complex (PIC) formation is one of the most powerful techniques for obtaining molecular self-assemblies in aqueous media. The simple preparation process based on multiple electrostatic interactions is quite attractive for material syntheses, as well as biomedical applications. Therefore, it is desirable to control PIC architectures at the nanoscale in order to expand the scope of PIC materials. In this review article, recent progress on PIC vesicles (PICsomes) is summarized. PICsomes were first developed by my research group, and we recently succeeded in controlling the sizes and structural uniformity of the vesicles. Furthermore, the characteristic dynamic nature of PICs was revealed: PICs were found to exhibit reversible association/dissociation and structural transformation. We demonstrated that crosslinking the PIC layers of PICsomes is a powerful method for tuning properties such as stability and permeability. Finally, the potential utility of PICsomes for drug delivery nanocarriers was examined, and their future biomedical application is discussed..
25. Daisuke Kokuryo, Yasutaka Anraku, Akihiro Kishimura, Sayaka Tanaka, Mitsunobu R. Kano, Jeff Kershaw, Nobuhiro Nishiyama, Tsuneo Saga, Ichio Aoki, Kazunori Kataoka, SPIO-PICsome: Development of a highly sensitive and stealth-capable MRI nano-agent for tumor detection using SPIO-loaded unilamellar polyion complex vesicles (PICsomes), JOURNAL OF CONTROLLED RELEASE, 10.1016/j.jconrel.2013.03.016, 169, 3, 220-227, 2013.08, Size controllable polyion complex vesicles (PICsomes), composed of biocompatible poly(ethylene glycol) (PEG) and poly(amino acid) s, have an extremely prolonged lifetime in the bloodstream that enables them to accumulate effectively in tumors via the enhanced permeability and retention (EPR) effect. The purpose of this study was to use PICsomes to synthesize a highly sensitive MRI contrast agent for more precise tumor detection. We synthesized SPIO-Cy5-PICsomes (superparamagnetic iron oxide nanoparticle-loaded Cy5-cross-linked Nano-PICsomes) and characterized them using dynamic light scattering and transmission electron microscopy in vitro and evaluated their ability to detect subcutaneously grafted tumors in vivo with MRI. The transverse relaxivity (r(2)) of the SPIO-Cy5-PICsomes (r(2) = 663 +/- 28 mM(-1) s(-1)) was 2.54 times higher than that of bare clinically-used SPIO. In in vivo MRI experiments on mice subcutaneously grafted with colon-26 tumor cells, the tumor signal was significantly altered at 3 h after SPIO-Cy5-PICsome administration and persisted for at least 24 h. Small and early-stage in vivo tumors (3 days after grafting, approximately 4 mm(3)) were also clearly detected with MRI. SPIO-loaded PICsomes are sensitive MRI contrast agents that can act as a powerful nanocarrier to detect small tumors for early diagnosis. (C) 2013 Elsevier B. V. All rights reserved..
26. N. Sasaki, M. Tatanou, Y. Anraku, A. Kishimura, K. Kataoka, K. Sato, Characterization of nanoparticle permeability on a membrane-integrated microfluidic device, MicroTAS, 3, 1818-1820, 2013.08.
27. Hidehiro Oana, Mutsuki Morinaga, Akihiro Kishimura, Kazunori Kataoka, Masao Washizu, Direct formation of giant unilamellar vesicles from microparticles of polyion complexes and investigation of their properties using a microfluidic chamber, Soft Matter, 10.1039/c3sm00089c, 9, 22, 5448-5458, 2013.06, Although hollow microscopic capsules have a variety of potential biomedical applications, reports of organic-solvent-free methods for their preparation are rather limited. Herein, a novel approach is demonstrated for organic-solvent-free preparation of giant unilamellar vesicles utilizing the unique response of polyion complexes (PICs) to changes in additive salt concentration. A microfluidic device consisting of a main channel bearing side pockets that work as microscale reaction chambers is designed for facilitating the preparation process under an optical microscope. With this device, real-time observation of morphological transformation of individual PIC microparticles is carried out during rapid reduction of the additive salt concentration and direct formation of giant vesicles from PIC microparticles is shown. There is a quasilinear relationship between the surface areas of the formed vesicles and the volumes of the PIC microparticles, and the thickness of the vesicle membrane estimated by the relationship is indicative of the formation of a uniform unilamellar structure of the PIC membrane. Furthermore, detailed properties of the formed PIC vesicles with regard to salt response, loading of guest molecules, and permeability of the PIC membrane with/without modification of the PIC membrane by cross-linking are investigated using the microfluidic chamber. Thus, the usefulness of the microfluidic chamber for visualization and investigation of dynamic responses of microscale soft materials during changes in surrounding conditions is also demonstrated..
28. Yasutaka Anraku, Akihiro Kishimura, Yuichi Yamasaki, Kazunori Kataoka, Living Unimodal Growth of Polyion Complex Vesicles via Two-Dimensional Supramolecular Polymerization, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 10.1021/ja3096587, 135, 4, 1423-1429, 2013.01, Understanding the dynamic behavior of molecular self-assemblies with higher-dimensional structures remains a key challenge to obtaining well-controlled and monodispersed structures. Nonetheless, there exist few systems capable of realizing the mechanism of supramolecular polymerization at higher dimensions. Herein, we report the unique self-assembling behavior of polyion complexes (PICs) consisting of poly(ethylene glycol)-polyelectrolyte block copolymer as an example of two-dimensional supramolecular living polymerization. Monodispersed and submicrometer unilamellar PIC vesicles (nano-PICsomes) displayed time-dependent growth while maintaining a narrow size distribution and a unilamellar structure. Detailed analysis of the system revealed that vesicle growth proceeded through the consumption of unit PICs (uPICs) composed of a single polycation/polyanion pair and was able to restart upon the further addition of isolated uPICs. Interestingly, the resulting vesicles underwent dissociation into uPICs in response to mechanical stress. These results clearly frame the growth as a two-dimensional supramolecular living polymerization of uPICs..
29. Kensuke Osada, Horacio Cabral, Yuki Mochida, Sangeun Lee, Kazuya Nagata, Tetsuya Matsuura, Megumi Yamamoto, Yasutaka Anraku, Akihiro Kishimura, Nobuhiro Nishiyama, Kazunori Kataoka, Bioactive Polymeric Metallosomes Self-Assembled through Block Copolymer-Metal Complexation, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 10.1021/ja304615y, 134, 32, 13172-13175, 2012.08, Spontaneous formation of polymeric metallosomes with uniform size (similar to 100 nm) was found to occur in aqueous medium through the reaction of an anticancer agent, (1,2-diaminocyclohexane)platinum(II) (DACHPt), with a Y-shaped block copolymer of omega-cholesteroyl-poly(L-glutamic acid) and two-armed poly(ethylene glycol) (PEGasus-PLGA-Chole). Circular dichroism spectrum measurements revealed that the PLGA segment forms an alpha-helix structure within the metallosomes, suggesting that secondary-structure formation of metallocomplexed PLGA segment may drive the self-assembly of the system into vesicular structure. These metallosomes can encapsulate water-soluble fluorescent macromolecules into their inner aqueous phase and eventually deliver them selectively into tumor tissues in mice, owing to the prolonged blood circulation. Accordingly, fluorescent imaging of the tumor was successfully demonstrated along with an appreciable antitumor activity by DACHPt moieties retained in the vesicular wall of the metallosomes, indicating the potential of metallosomes as multifunctional drug carriers..
30. Yasutaka Anraku, Akihiro Kishimura, Atsushi Kobayashi, Makoto Oba, Kazunori Kataoka, Size-controlled long-circulating PICsome as a ruler to measure critical cut-off disposition size into normal and tumor tissues, Chemical Communications, 10.1039/c1cc11465d, 47, 21, 6054-6056, 2011.06, Selective disposition of nanocarriers into target tissue is an essential issue in drug delivery. Critical size of nanocarriers (∼150 nm) discriminating the permeability into normal and tumor tissues was determined by the use of size-tunable, polyion complex hollow vesicles (PICsome) as a ruler..
31. Yasutaka Anraku, Akihiro Kishimura, Makoto Oba, Yuichi Yamasaki, Kazunori Kataoka, Spontaneous Formation of Nanosized Unilamellar Polyion Complex Vesicles with Tunable Size and Properties, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 10.1021/ja908350e, 132, 5, 1631-1636, 2010.02, Fabrication of monodispersed, submicrometer-sized vesicles (nanosomes) that form through self-assembly possessing a thin and permeable membrane remains a significant challenge. Conventional fabrication of nanosomes through self-assembly of amphiphilic molecules often requires cumbersome processes using organic solvents combined with physical procedures (e.g., sonication, thermal treatment, and membrane filtration) to obtain unilamellar structures with a controlled size distribution. Herein, we report the first example of spontaneously formed submicrometer-sized unilamellar polyion complex vesicles (Nano-PICsomes) via self-assembly of a pair of oppositely charged PEG block aniomer and homocatiomer in an aqueous medium. Detailed dynamic light scattering and transmission electron microscopic analysis revealed that vesicle sizes can be controlled in the range of 100-400 nm with a narrow size distribution, simply by changing the total polymer concentration. Also, each Nano-PICsome was composed of a uniform single PIC membrane, the thickness of which is around 10-15 nm, regardless of its size. Fluorescence correlation spectroscopy measurement verified that Nano-PICsomes were able to encapsulate water-soluble fluorescent macromolecules in the inner water phase and release them slowly into the exterior. Moreover, cross-linking of the vesicle membrane allows tuning of permeability, enhancement in stability under physiological conditions, and preservation of size and structure even after freeze-drying and centrifugation treatment. Finally, Nano-PICsomes showed a long circulation time in the bloodstream of mice. Precise control of the particle size and structure of hollow capsules through simple aqueous self-assembly and easy modification of their properties by cross-linking is quite novel and fascinating in terms of ecological, low-cost, and low-energy fabrication processes as well as the potential utility in the biomedical arena..
32. Hidehiro Oana, Akihiro Kishimura, Kei Yonehara, Yuichi Yamasaki, Masao Washizu, Kazunori Kataoka, Spontaneous formation of giant unilamellar vesicles from microdroplets of a polyion complex by thermally induced phase separation, Angewandte Chemie - International Edition, 10.1002/anie.200900721, 48, 25, 4613-4616, 2009.06, Water pump: Polyion complex (PIC) vesicles are spontaneously formed from PIC microdroplets, which are formed by mixing cationic and anionic polymers (see picture). The formation process can be reversibly controlled by local heating with a focused infrared laser that triggers microphase separation and subsequent water influx. The size of the resulting giant unilamellar vesicles is determined by the initial size of the PIC droplets..
33. Wen-Fei Dong, Akihiro Kishimura, Yasutaka Anraku, Sayan Chuanoi, Kazunori Kataoka, Monodispersed Polymeric Nanocapsules: Spontaneous Evolution and Morphology Transition from Reducible Hetero-PEG PICmicelles by Controlled Degradation, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 10.1021/ja808419b, 131, 11, 3804-+, 2009.03, In this communication, a novel "self-templating" strategy was used to prepare uniform and biocompatible nanocapsules by the addition of a reduction agent (i.e., DTT) into a solution of highly monodispersed PICmicelles bearing a heterodetachable PEG Corona. PEG chains were released from PICmicelle shells following disulfide reduction which leads a spontaneous and drastic morphology evolution from micelles to vesicles induced by the decrease of the PEG weight fraction. Formation of uniform nanocapsules with controllable capsule size was achieved by careful control of the micelle composition and molecular weight of homo-P[Asp(DET)]..
34. Akihiro Kishimura, Sittipong Liamsuwan, Hiroyuki Matsuda, Wen Fei Dong, Kensuke Osada, Yuichi Yamasaki, Kazunori Kataoka, PH-dependent permeability change and reversible structural transition of PEGylated polyion complex vesicles (PICsomes) in aqueous media, Soft Matter, 10.1039/b815884c, 5, 3, 529-532, 2008.11, The acidic pH-sensitivity of polyion complex vesicles (PICsomes) was investigated, using dynamic light scattering (DLS) and confocal laser scanning microscopy (CLSM). PICsomes showed pH-dependent and reversible structural transition, and also underwent a change in permeability by sensing acidic pH. Increased membrane permeability at the pH corresponding to cellular endosomes may be useful for future applications of PICsomes as a delivery vehicle of biologically active compounds to intracellular compartment..
35. Akihiro Kishimura, Aya Koide, Kensuke Osada, Yuichi Yamasaki, Kazunori Kataoka, Encapsulation of myoglobin in PEGylated polyion complex vesicles made from a pair of oppositely charged block ionomers
A physiologically available oxygen carrier, Angewandte Chemie - International Edition, 10.1002/anie.200701776, 46, 32, 6085-6088, 2007.08, (Figure Presented) Take your PIC: Biologically active polyion complex vesicles (PICsomes) with encapsulated myoglobin (Mb) can be prepared by the self-assembly of a pair of oppositely charged block ionomers with polyethylene glycol (PEG) segments (see picture; metMb: metmyoglobin). The loaded Mb maintains reversible oxygenation even in the presence of trypsin..
36. A Koide, A Kishimura, K Osada, WD Jang, Y Yamasaki, K Kataoka, Semipermeable polymer vesicle (PICsome) self-assembled in aqueous medium from a pair of oppositely charged block copolymers: Physiologically stable micro-/nanocontainers of water-soluble macromolecules, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 10.1021/ja057993r, 128, 18, 5988-5989, 2006.05.
37. Akihiro Kishimura, Takashi Yamashita, Kentaro Yamaguchi, Takuzo Aida, “Rewritable phosphorescent paper” by the control of competing kinetic and thermodynamic self–assembling events., Nature Materials, 10.1038/nmat1401, 4, 7, 546-549, 2005.07.
38. Akihiro Kishimura, Takashi Yamashita, Takuzo Aida, Phosphorescent Organogels via 'Metallophilic' Interactions for Reversible RGB–Color Switching, Journal of the American Chemical Society, 10.1021/ja0441007, 127, 1, 179-183, 2005.01.
Presentations
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1. ○Ziwei Ma, Hiroshi Kamizawa, Rento Ota, Teruki Nii, Takeshi Mori,Yoshiki Katayama,　Akihiro Kishimura, Development of temperature-responsive polyion complexes capableof sustained release of micelles containing oligonucleotides, 日本薬学会第143年会, 2023.03.
2. ○Haya Kadan, Biplab K C, Teruki Nii, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Protein encapsulation in polypeptide-based cyto-mimetic complex coacervates via polymer charge-density tuning, 日本薬学会第143年会, 2023.03.
3. 〇Fadlina Aulia, Teruki Nii, Takeshi Mori, Yoshiki Katayama, Kishimura Akihiro, Application of sulfobetaine-type zwitterionic polymer for drug absorption enhancer in transmucosal drug delivery, 日本薬学会第143年会, 2023.03.
4. ◯Akihiro Kishimura, Development of Synthetic Polypeptide-based Biocondensates for Artificial Cells and Biomedical Materials, 3rd International Symposium of Chemistry Education Center for Sustainability: Frontiers in Chemical Biology for Sustainable Future, 2022.11.
5. 〇Fadlina Aulia, Hiroaki Matsuba, Teruki Nii, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Development of polypeptide-based polyion complex (PIC) nanoparticles with tunable physicochemical nature for selective cellular interaction and manipulation of cellular function, 第71回高分子討論会, 2022.09.
6. ◯Akihiro Kishimura, Chemistry for Medicine: a Common Language
Based on the “World of Molecules”~Toward Artificial Viruses and Cells~, Kick-off Symposium of the Kyushu University Stockholm Liaison Offic: Finding a Common Language for Well-Being and Inclusive Growth: Connecting Medicine, Chemistry, and Sustainability Science, 2022.08.
7. ○T. Yamada, T. Maruyama, Biplab KC, T. Nii, T. Mori, Y. Katayama, A. Kishimura, Development of a novel polyion complex with a hierarchical structure formed utilizing phase separation from a condensate, IUMRA-ICYRAM 2022, 2022.08.
8. 〇H. Kamizawa, T. Maruyama, Y. Liu, T. Nii, K. Miyata, T. Mori, Y. Katayama, A. Kishimura, Development of novel higher order structures of polyion complexes obtained via polymer condensate formation, IUMRA-ICYRAM 2022, 2022.08.
9. ○Fadlina Aulia, Teruki Nii, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Tuning of polyion complex(PIC) properties for effective modulation of cellular uptake behaviors of PIC-based nanoparticles having different block copolymer compositions and chemical modifications on charges functionality, IUMRA-ICYRAM 2022, 2022.08.
10. ○劉　一イ、片山佳樹、森　健、岸村　顕広, Formation and structure control of a protein-loaded yolk-shell polyion complex, IUMRA-ICYRAM 2022, 2022.08.
11. ○岸村　顕広, Development of novel polyioncomplex systems for protein delivery and encapsulation, VANJ Conference 2020, 2020.11.
12. 岸村顕広, The Trust Bridge between Societies and Scientists, Implementation of Novel Technologies to Our Society, webinar lectures collaborating with NRC in Egypt and JSPS Research Station, 2020.08.
13. ○Buplab K.C Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Rational Design Of An Artificial Liquid-liquid Phase Separation System Via Side-chain Modification Of Synthetic Polypeptide As IDP Mimic For Effective Protein Sequestration, 第20回日本蛋白質科学会年会, 2020.07.
14. ○丸山朋輝、劉一イ、森健、片山佳樹、岸村顕広, Osmotic Pressure-induced Multilamellar Structure Formation Based On Protein-Encapsulated Yolk-shell Structure , 第20回日本蛋白質科学会年会, 2020.07.
15. アウリア ファドリナ、森 健、片山 佳樹、岸村 顕広、森本 展行、山本 雅哉, 経粘膜薬物送達に向けたスルホベタインポリマーの浸透能力評価, 日本薬学会第140年会（京都）, 2020.03.
16. ○MengJu Chan, Kanjiro Miyata, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Facile Fabrication of Silica-Hybrid Polyion Complex Nano-Vesicles and Its Function Enhancement, the 18th Asian Chemical Congres, 2019.12.
17. ○Biplab K C, Takeshi Mori、Akihiro Kishimura、Yoshiki Katayama, Protein Sequestration in Synthetic Di-block-copolymer-based Complex Coacervate by Mimicking Intracellular Phase Separation, the 18th Asian Chemical Congres, 2019.12.
18. ○Biplab K C, Takeshi Mori、Akihiro kishimura、Yoshiki Katayama, Polypeptide based complex coacervate as biomimetic material to sequester biomolecules via rational design of polymeric sidechain, Okinawa Colloids 2019, 2019.11.
19. ○Fadlina Aulia, 松葉弘顕、中瀬生彦、森　健、岸村顕広、片山佳樹, Control of cellular uptake behavior based on tuning of structure and physical properties of PEGylated polyion complex and its application, Okinawa Colloids 2019, 2019.11.
20. ○劉　一イ、森　健、片山佳樹、岸村　顕広, Formation of yolk-shell structure based on self-assembly of polyions and proteins, Okinawa Colloids 2019, 2019.11.
21. ○T. Egashira, T. Mori, Y. Katayama and A. Kishimura, Release of Metal Nanoparticles as Micelles from Complex Coacervates Nano-Architectures, Okinawa Colloids 2019, 2019.11.
22. ○Akihiro Kishimura, Block-copolymer-based polyion complex nanostrucctures as a platform for incorporation of colloidal nanomaterials, Okinawa Colloids 2019, 2019.11.
23. ○Akihiro Kishimura, Control of the Formation Process of Polypeptide Self-assemblies for Understanding Complex Biological Systems:From Nano-physiology to Artificial Cells, Japan-Britain Joint Symposiumu, 2019.09.
24. ○Fadlina Aulia, Morimoto Nobuyuki, Mori Takeshi, Katayama Yoshiki, Kishimura Akihiro, Development of cell penetrating materials for transmucosal drug delivery, 第56回化学関連支部合同九州大会, 2019.07.
25. ○Biplab K C, Takeshi Mori、Akihiro Kishimura、Yoshiki Katayama, Synthetic complex coacervate to sequester functional proteins: A synthetic model for intracellular phase separation, 第19回日本蛋白質科学会年会・第71回日本細胞生物学会大会 合同年次大会, 2019.06.
26. ○岸村　顕広, Block-copolymer-based polyion complex nanotechnology as a platform for biomedical appliactions, China-Japan-Singapore Joint Symposium on Supramolecular Systems and Optoelectronic Functions, 2019.06.
27. ○Biplab.K.C, T.Mori, Y.Katayama, A.Kishimura, Introduction of Charge Heterogeneity in Di-block Copolymer for Effective Protein Sequestration in Coacervates, 第24回日本化学会九州支部・韓国化学会釜山支部合同セミナー, 2019.06.
28. ○江頭巧、森健、片山佳樹、岸村顕広, Incorporation of Metal Nanoparticles into Complex Coacervates Nano-Architectures and Their Release via Structural Transformation, 第24回日本化学会九州支部・韓国化学会釜山支部合同セミナー, 2019.06.
29. ○Akihiro Kishimura, Development of biomedical and biomimetic materials
utilizing polyion-complex-based nanofabrication techniques, 2019.05.
30. ○Akihiro Kishimura, Development of biomedical and biomimetic materials
utilizing polyion-complex-based nanofabrication techniques, 2019.05.
31. ○Akihiro Kishimura, Block-copolymer-based polyion complex nanotechnology as a platform for biomedical applications, 2019.05.
32. ○Akihiro Kishimura, Block-copolymer-based polyion complex nanotechnology as a platform for biomedical applications, 2019.05.
33. ○Y. Liu, T. Mori, Y. Katayama, A. Kishimura, Yolk-Shell assembly formation based on polyion complex of proteins, ACS National Meetin & Expo, 2019.04.
34. ○Y. Liu, T. Mori, Y. Katayama, A. Kishimura, Yolk-Shell assembly formation based on polyion complex of proteins, ACS National Meetin & Expo, 2019.04.
35. ○Akihiro Kishimura, Yiwei Liu, Biplab KC, Takumi Egashira, Takeshi Mori, Yoshiki Katayama, , Block-copolymer-based polyion complexes for utilization of proteins and inorganic nanoparticles, 257th ACS National Meeting, Division of Polymer Chemistry, Polymer-Based Gene & Drug Delivery Systems,, 2019.03.
36. ○B. S. Kim, S. Chuanoi, Y. Anraku, K. Miyata, A. Kishimura, K. Kataoka, , siRNAsome: A self-assembled vesicular architecture formed from siRNAs and PEGylated block catiomers, 6th International Conference on Multifunctional, Hybrid and Nanomaterials 2019,, 2019.03.
37. ○Biplab K C, Takeshi Mori、Akihiro Kishimura、Yoshiki Katayama, Functionalization of polyelectrolyte side chains via chemical modification for effective sequestration of biomolecules into diblock-copolymer-based complex coacervate, 第28回日本MRS年次大会, 2018.12.
38. ○Takumi Egashira・Takeshi Mori・Yoshiki Katayama・Akihiro Kishimura, Thermo-responsive structural transition of nano-structured polyion complexes using, IPC2018, 2018.12.
39. ○松葉 弘晃、中瀬 生彦、森　健、片山佳樹、岸村　顕広, Utilization of dynamic response of polyion complex for enhancing cell-communication function of nanomedicine, IPC2018, 2018.12.
40. ○Biplab K.C, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Sequestration of biomolecules into diblock-copolymer-based coacervate through chemical modification of polyelectrolyte sidechain, IPC2018, 2018.12.
41. ○B. S. Kim, K. Miyata, A. Kishimura, K. Kataoka, Vesicular self-assemblies from siRNAs and PEGylated block catiomers (siRNAsomes): Their structural, physicochemical, and biological characteristics,, IPC2018, 2018.12.
42. Akihiro Kishimura, Development of therapeutic nanosystems based on well-designed nano-scaled formulations, 国立中興大学・短期訪問学者セミナー, 2018.10.
43. Akihiro Kishimura, Polymer nanotechnology for advanced materials: self-assembly and integration of macromolecules and colloidal nanoparticles, 国立中興大学・短期訪問学者セミナー, 2018.10.
44. Wararu Hatanaka, Hiroki Takeuchi, Akihiro Kishimra, Yoshiki Katayama, ○Takeshi Mori, Modification of Transmembrane Protein Mimics on Living Cells, The 79th Okazaki Conference, 2018.09.
45. ○Akihiro Kishimura, Polymer-nanobiotechnology for Utilization of Proteins Towards Biomedical Application, 第35回国際フォトポリマーコンファレンス　ICPST-35(2018), 2018.06.
46. ○劉　一イ、森　健、片山佳樹、岸村　顕広, 高効率にタンパク質内包が可能なポリイオンコンプレックスyolk-shell構造体の開発, 第34回日本DDS学会学術集会, 2018.06.
47. ○劉　一イ、濱田　祐次朗、森　健、片山佳樹、岸村　顕広, ポリイオンコンプレックス形成に基づくタンパク質内包自己組織化yolk-shell構造の開発, 第67回高分子学会年次大会, 2018.05.
48. ○小川敦嗣、唐 蘅敏、森健、片山佳樹、岸村顕広, Development of functionalized PEGylated polymer vesicles for overcoming the mucosal barrier, ISBC2017, 2017.12.
49. Mikio Terauchi1, ○Biplab KC, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Study on functional biomolecule incorporation in complex coacervates using
PEG-based block copolymers, ISBC2017, 2017.12.
50. Akihiro Kishimura, Development of
Polyion Complex Vesicle (PICsome)
for Biomedical Applications, 理研セミナー, 2017.12.
51. 濱田　祐次朗、尚山　堅士郎、森　健、 片山　佳樹、○岸村　顕広, 双親水性ブロック共重合体を用いたいナノ構造化コア
セルベートへの機能性ナノ粒子の部位選択的導入, 第27回日本MRS年次大会, 2017.12.
52. 寺内　幹雄、Biplab KC、森　健、片山　佳樹、 ○岸村　顕広, 高分子電解質との複合コアセルベート形成に基づくタ
ンパク質の特異な自己組織化挙動, 第27回日本MRS年次大会, 2017.12.
53. ○濱田祐次朗、檜垣勇次、小椎尾　謙、高原　淳、森　健、片山佳樹、岸村顕広, ブロック共重合体を用いたナノ構造化コアセルベートの設計とミクロな構造とマクロな物性の相関関係の解明, 第27回日本MRS年次大会, 2017.12.
54. ○小川敦嗣、唐 蘅敏、森健、片山佳樹、岸村顕広, 粘膜バリア突破を目指した膜機能強化型PEG化ポリ
マーベシクルの開発, 第27回日本MRS年次大会, 2017.12.
55. 〇松葉弘晃、小川敦嗣、唐 蘅敏、山崎北斗、森健、片山佳樹、岸村顕広, 標的組織送達後の機能発現を指向したPEG化ポリイオンコンプレックスナノ粒子の細胞取り込み挙動制御：その粒子形態とPEG鎖長への依存性評価, 第27回日本MRS年次大会, 2017.12.
56. ○松葉弘晃、唐蘅敏、小川敦嗣、森健、片山佳樹、岸村顕広, 標的組織送達後の機能発現に向けたPEG化ポリイオンコンプレックスナノ粒子の細胞取り込み挙動制御：その粒子形態・PEG鎖長依存性, 平成２９年度高分子学会九州支部特別講演会, 2017.11.
57. ○Akihiro Kishimura, Facile Synthesis of Nano-structured Materials Based on Block Copolymer Technology and their Biomedical Applications, 2017International Conference on smart Science, 2017.04.
58. Akihiro Kishimura, Development of Polymer-Based Supramolecular Nanosystems for Therapeutic Applications, The 50th CMS International Seminar, 2017.03.
59. ○Akihiro Kishimura, Kenshiro Naoyama, Yujiro Hamada, Takeshi Mori, Yoshiki Katayama, Development of nanostructured coacervates based on double hydrophilic block copolymers and the behavior of site-selective incorporation of functional nanoparticles, 日本化学会第97春季年会, 2017.03.
60. ○Yiwei Liu, Hengmin Tang, Takeshi Mori, Yoshiki Katayama and Akihiro Kishimura, Enhanced Protein Encapsulation by Polyion Complex Vesicle Induction on Protein-Polyion Complex Particle, The 11th SPSJ International Polymer Conference (IPC2016), 2016.12.
61. ○Yujiro Hamada, Takeshi Mori, Yoshiki Katayama and Akihiro Kishimura, Design of nano-structured PICs using all-hydrophilic block copolymers and their site-specific incorporation of functional nanomaterials, The 11th SPSJ International Polymer Conference (IPC2016), 2016.12.
62. ○岸村顕広, Development of Self-assembled Nano-structured Materials for Biomedical Applications, MRS-id Meeting 2016, 2016.10.
63. Akihiro Kishimura, Development of nanostructured soft materials
based on all-hydrophilic block copolymers: From basics to biomedical applications,, 2016.10.
64. ○岸村顕広, Development of Novel Supramolecular Hollow Capsules "Picsomes" for Biomedical Applications, ChinaNanomedicine 2016, 2016.10.
65. ○岸村顕広, Development of polyion complex nanostructures based on all-hydrophilic block copolymers, Ostwald Colloquium 2016, 2016.09.
66. Akihiro Kishimura, Development of polymeric nano-vesicles for advanced drug delivery system, 2016.08.
67. Akihiro KISHIMURA, Engineering of Enzyme Nano-capsules for Biomedical
Applications, CIMTEC2016, 2016.06.
68. Akihiro Kishimura, Development of polymeric nanomedicine for novel therapy and pathophysiologic study, 2016.05.
69. Yuki Sakamura , Hengmin Tang , Takeshi Mori , Yoshiki Katayama , ○Akihiro Kishimura, Development of enzyme-loaded polymeric nanocapsules as a versatile platform for enzyme applications: Effect of co-encapsulation of neutral macromolecules, THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015, 2015.12.
70. ○Kenshiro Naoyama, Takeshi Mori , Yoshiki Katayama , Akihiro Kishimura, Site-selective incorporation of nanoparticles into the complex coacervate utilizing self-assembly of block copolymers in aqueous solution, THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015, 2015.12.
71. ○Akihiro Kishimura, Development of polyion complex vesicle “PICsomes” and their unique self-assembling behavior, THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015, 2015.12.
72. ○Hengmin Tang, Yuki Sakamura , Satoshi Tanaka , Takeshi Mori , Yoshiki Katayama , Akihiro Kishimura, Development of a novel enzymatic nano-reactors as biodetoxification nanomedicine, THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015, 2015.12.
73. Akihiro Kishimura, Rational design of polyion complex nano-architectures for development of functional materials, 2015 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (17th) and Fibers (15th), 2015.11.
74. Akihiro Kishimura, Development of polyion complex nano-vesicles for biomedical applications, Polymers in Medicine and Biology:2015, 2015.09.
75. ○唐　衡敏、森　健、田中　智之、片山佳樹、岸村　顕広, Development of a novel enzymatic nano-reactor for the application of biodetoxification, 42nd CRS Annual Meeting & Exposition, 2015.07.
76. ○唐　衡敏、森　健、田中　智之、片山佳樹、岸村　顕広, Development of enzymatic nano-reactor for removing physiological active substance, 第64回高分子学会年次大会, 2015.05.
77. Akihiro Kishimura, Development of Polyion Complex Vesicles “PICsomes” with Semipermeable Properties As a Novel Platform for Nano-medicine, The 5th International Conference on the Development of Biomedical Engineering in Vietnam, 2014.06.
78. Akihiro Kishimura, Novel method to load high amount of drugs and macromolecules into polyion complex vesicles (PICsomes), 40th Annual Meeting & Exposition of the Controlled Release Society, 2013.07.
Membership in Academic Society
  • Japanese Association for the Advancement of Science
  • Japan Epidemiological Association
  • The Biophysical Society of Japan
  • Protein Science Society of Japan
  • Japanese Society for Biomaterials
  • The Japan Society of Drug Delivery System
  • Controlled Release Society
  • American Chemical Society
  • The Materials Research Society of Japan
  • The Society for Polymer Science, Japan
  • The Chemical Society of Japan
  • The Pharmaceutical Society of Japan
Educational
Educational Activities
Classes: Bio-organic chemistry (2013-2015), Biochemistry II (2016-), Molecular Biology (2015-2021), Organic Chemistry I (2022)
Bio-nanotechnology (2014-2016)、Chemistry for Medicine I　(2017-), Chemistry for Medicine II (2017-), Life Engineering I/II (2017-)
Other Educational Activities
  • 2022.06, I designed, planned, and implemented the following course as an elective course for the Kyushu University SPRING program (Future-Creation course).
    ________
    [6] Internationality Courses
    (IV) Global Young Academy
    Theme: Young Generation’s Thought on Relationship between the Future Society and Academia

    In June 2022, the 12th International Conference of Young Scientists and GYA Annual General Meeting (GYA AGM2022) will be held at Kyushu University. Utilizing this opportunity, special programs are provided to the course students to develop their international skills and gain a bird’s-eye view of the field across multiple disciplines.

    Course requirements: Students must complete the following programs (a) and (b).
    Program (a): Attend the following three online sessions of GYA AGM2022 and prepare a report on each. (Submission deadline: June 30th, 2022)
    (i) June 15th (Wed, 17:00-18:30) Panel Discussion “Harmonizing Reason with Sensibility from the viewpoint of citizen science: Aiming to build a social system that promotes citizen science”
    (ii) June 15th (Wed, 19:00-20:30) Plenary Session 1 “Harmonizing Reason with Sensibility to Enable Sustainable and Inclusive Society: Universities in the Future Society – To be a public platform for realizing a sustainable and inclusive society (tentative)”
    (iii) June 16th (Thu, 19:00-20:30) Plenary Session 2 “How much creativity is valued in your area of science?”– How might we contribute better to the society through harmonizing reason with sensibility?”
    * Whenever possible, participate on-site at the Opening Ceremony (June 14, 17:00-, at Shiiki Hall), and participate online at the Closing Ceremony (June 17, 17:00-).
    Language: English

    Program (b): Participate in side events held parallel to the international conference. Participate in one of Course 1 or Course 2. (Participation in both courses is possible. If you want to do so, please contact the person in charge of [6]-(IV)[AK1] ).

    (Course 1) English Debate Workshop for High School Students, University Students and Next Generation Researchers
    Language: English
    Number of students accepted: 8 (If more than 8 students apply, selection will be made by lottery, or by considering a research background of the student and a topic the student prioritizes.)
    Course requirements: Participate in and complete a pre-conference lecture (online, the date to be announced) and a debating session (June 14 (Tue), 10:20-16:00 at Shiiki Hall). (Submission deadline of mini-report or assignments will be announced later.)
    Note that those who have no experience in debating are welcome to participate. (The Pre-Lecture is a course for inexperienced debaters. Experienced debaters are of course welcome.)

    (Course 2)
    1. “The Society of 30 Years from Now: A Science Fiction Prototyping Workshop for the Younger Generation” June 15th (Wed, 12:30-16:00m, online).
    2. “A Dialogue Between Young Researchers and the Next Generation of Researchers” June 16th (Thu, 13:00-16:00 at Shiiki Hall)
    Language: Japanese
    Number of students accepted: ~10 students. If there are too many applicants, a lottery may be held.
    Course requirements: Participate in and complete both events. (Submission deadline of mini-reports and questionnaire: June 30th, 2022)
    *Detailed description of each course is given in the following pages.

    Detailed program description (Syllabus)
    [Program (a)]
    Participate in the following three sessions and submit a one-page, A4-size report on what you have thought about regarding the session topics, as you reflect on your research, life to date, etc. (Submission deadline: June 30th, 2022)

    (i) Panel Discussion “Harmonizing Reason with Sensibility from the viewpoint of citizen science: Aiming to build a social system that promotes citizen science”
    Day & Time: June 15th (Wed, 17:00-18:30)
    Event Format: Online meeting (zoom)
    Session description: Citizen science is a scientific activity conducted by the general public and is expanding worldwide. In Japan, however, its social system is not ready to promote citizen sciences; in other words, it is immature in terms of co-creation of research projects among professional researchers, the general public and policymakers, conducting research together, and implementing research outcomes to the society. Therefore, the Young Academy of Japan (YAJ) compiled a list of issues that need to be resolved in implementing citizen sciences in Japanese society compared to other countries and released the recommendation in 2020. In this session, we will introduce the recommendation issued by YAJ in 2020 and the trends in Japan afterward. Also, the global trend of citizen science will be overviewed, together with some important examples. Finally, we will discuss and clarify the significance and challenges of Citizen Science as a key tool to support society in various situations, especially in achieving the SDGs, and how to construct a social system to promote it.

    (ii) Plenary Session 1 “Harmonizing Reason with Sensibility to Enable Sustainable and Inclusive Society: Universities in the Future Society – To be a public platform for realizing a sustainable and inclusive society (tentative)”
    Day & Time: June 15th (Wed, 19:00-20:30)
    Event Format: Online meeting (zoom)
    Session description: Universities are the only public places that can strongly connect the daily developing academia to the society. In this plenary session, we will consider the rebalancing of "reason" and "sensibility," and consider the university as a place that connects with the public. In particular, we will have speeches from Dr. Juichi Yamagiwa (the Director-General of the Research Institute for Humanity and Nature), who served as the president of the Science Council of Japan on the theme of strengthening the connection between academia and society through dialogue, and Mr. Oriza Hirata (the president of Professional College of Arts and Tourism), who links education, art and culture to the community through dialogue. In addition, Prof. Takeshi Sakai (Kyushu University) will present a case study of Kyushu University's Ito Campus, which was designed and developed with consideration for the sustainability of the local environment and inclusion of the local community. Finally, in the panel discussion, we will discuss the role of academia and universities in the era of SDGs and post-SDGs.

    (iii) Plenary Session 2 “How much creativity is valued in your area of science?”– How might we contribute better to the society through harmonizing reason with sensibility?”
    Day & Time: June 16th (Thu, 19:00-20:30)
    Event Format: Online meeting (zoom)
    Session description: How does society perceive scientists? How does it vary from country to country and field to field? How can scientists contribute more to society? To consider these questions, we are conducting a survey among GYA members and alumni. The session will provide an opportunity for discussion based on the survey result, presentations given by the speakers from different fields, and comments and questions from the audience. We would like to inform a wide range of participants about the discussions that GYA's Focus Area has been having and to have time to think together.

    [Program (b)]
    Complete either Course 1 or Course 2. (You may take both courses.)

    (Course 1)
    “English Debate Workshop for High School Students, University Students and Next Generation Researchers”
    The workshop will include a pre-conference online lecture and a debating session. Debates deal with topics such as "environment," "virtual reality," "medicine," and "international relations," inviting experts in each field to discuss topics that cannot be settled only with advanced knowledge. In addition, the experts will provide new perspectives through their presentations, and interactive discussions will be held to help students develop their future interdisciplinary research.

    1. Pre-conference Online Lecture: Debate Practices with SDGs
    Date & Time: TBA (2 periods in a single day)
    Event Format: Online lecture
    Lecturer: Prof. Toru Oga (Faculty of Law, Kyushu University), et al.
    Objective: The objective of this course is to learn about SDG topics (aligned with the 17 goals) through debates. Debates will be conducted by participants randomly assigned to the affirmative or negative side. This is compatible with the SDGs, which are oriented toward a multi-stakeholder approach, because it is necessary to organize the debate from the perspective of others (actors, stakeholders, and those involved in the debate topic) rather than from one's own position.
    Keywords: Debate, SDGs, Critical Thinking
    Teaching Materials: Textbook, reference books, printed materials/slide materials (available from Moodle)
    Textbook: Chihiro Nakagawa, “Improvised English Debate (PDA) in class”, ‎Parliamentary Debate Personnel Development Association (2017).
    https://pdpda.org/debate/educational-materials/
    Reference Books:
    Lewis Iwu, Words That Win: How to Win the Debates That Matter (John Catt Edu Ltd, 2019)
    加藤彰『即興型ディベートの教科書』（あさ出版, 2020）

    Course Plan:

    Course Theme
    Course Contents
    Pre/Post Study Contents
    1
    Debate Practice 1
    Explanation of Debate Rule, Debate Practice (Environment, AI issues)
    Reviewing Debate topic
    2
    Debate Practice 2
    Debate Practice (Medical and Development issues)
    Reviewing Debate topic


    Evaluation: Mini-Report, Class Participation
    (Submission deadline of mini-report or assignment will be announced later.)

    2. Debating session
    Date & Time: June 14 (Tue, 10:20-16:00)
    Event Format: Onsite meeting
    Venue: Lecture rooms, Shiiki Hall (Ito Campus)

    Program:
    (Morning session)
    English debates on four topics ((a) Environment, (b) Virtual Reality, (c) Medicine, and (d) International Relations/Development). Students will be the debaters; each team will consist of three debaters (each team will consist of one high school student, one university student, and one SPRING course student). The schedule will be as follows:
    Preparation time (15 minutes)
    Debating (20 minutes)
    Judges' commentary (15 minutes)
    Moderator’s presentation (about 30 minutes)
    Q&A and floor discussion (approx. 30 minutes)

    Guest Moderators:
    Theme (a). Environment
    Prof. Akira Mori (Research Center for Advanced Science and Technology, the University of Tokyo)
    Theme (b). Virtual Reality
    Prof. Kouta Minamizawa (Graduate School of Media Design, Keio University)
    Theme (c). Medicine
    Prof. Mitsunobu Kano (Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University)
    Theme (d). International Relations/Development
    Prof. Ryoko Nakano (Institute of Human and Social Sciences, Faculty of Law, Kanazawa University）

    (Afternoon session)
    　Two teams will be formed from the debaters who performed well in the morning session to debate on the theme of "SDGs". The program will proceed in the same way as the morning session.
    Guest Moderator: Prof. Jinhee Kim (Korean Educational Development Institute)

    Evaluation: Class Participation, Mini-Report & Questionnaire (Submission deadline: June 30th, 2022)

    (Course 2)
    1. “The Society of 30 Years from Now: A Science Fiction Prototyping Workshop for the Younger Generation”
    Date & Time: June 15th (Wed, 12:30-16:00)
    Event Format: Online meeting (zoom)
    Course Outline: Are your research activities, which were exciting at first, becoming routine and getting bored with your daily activities? You may want to revitalize your thinking, which tends to stop when you fall into a routine, or you may want to reevaluate where you stand, because your research itself is in a stagnant period and you are unsure of the value of your research. At such times, thinking about the future from a science fiction perspective may be a breakthrough. In fact, many overseas researchers and innovators have publicly stated that science fiction has helped them in their research and development. Would you like to participate in this SF prototyping where you can imagine a crazy future with various people and explore new research directions?
    Dohjin Miyamoto will coordinate the workshop as a leading expert in SF prototyping. You can sufficiently and effectively experience SF prototyping in an online format. On the day of the workshop, participants will work in groups of about 4-5 people, summarize the discussions and outcomes of each group, and finally submit a report.
    Overall Coordinator: Dohjin Miyamoto (Science Culture Writer)
    Reference: 『SFプロトタイピング ＳＦからイノベーションを生み出す新戦略』宮本道人、難波 優輝 、大澤 博隆・著、早川書房（2021）。
    Evaluation: Class Participation, Mini-Report & Questionnaire (Submission deadline: June 30th, 2022)


    2. “A Dialogue Between Young Researchers and the Next Generation of Researchers”
    Date & Time: June 16th (Thu, 13:00-16:00)
    Event Format: Onsite meeting
    Venue: Large Meeting Room, Shiiki Hall (Ito Campus)
    Course Outline: Discussions will be held to help the next generation of researchers recognize the importance of intergenerational connections across time and disciplines to create a better future intentionally. Together, we will consider how the next generation of researchers can make choices to shape the future society and environment while looking at the successes and failures piled up by the former generations. We will also discuss what kind of community of researchers is needed to encourage the next generation of researchers to make decisions for the future.
    A critical viewpoint to consider the relationship between society and science is the balance between understanding based on "reasons" and understanding based on "sensibility" or "emotions." This point is almost the same as the international conference's main theme, "Harmonising Reason with Sensibility​." Usually, humans have a discrepancy between reason and sensibility. There are cases in which what is rationally judged as "good" from a certain standpoint is unacceptable from the emotional viewpoint. Conversely, it is difficult to convey what is rationally judged to be "good" to others to empathize.
    In this event, we would like researchers of different generations to share some experiences of science communication issues for shaping a better future, examples of science communication to move people beyond reason through artistic approaches, and so forth. In addition, the researchers of the next generation will share their ideas and opinions through discussion and, finally, present outputs obtained in an interactive dialogue.

    Participants will work in groups of about 4-5 people on the day, summarize the discussions and outcomes of each group, and finally submit a report.

    Program (tentative):
    13:00–14:00 Presentations & messages from researchers話題提供（１時間程度）
    Speakers (tentative):
    Prof. Hitoshi Kuriyama (Faculty of Design, Kyushu University; about the topic on “Science & Art”)
    Prof. Nina Yasuda (Graduate School of Agricultural and Life Sciences, the University on Tokyo; about the topic on “Environment, Science and Society”)
    Prof. Motoi Wada (Faculty of Science and Engineering, Doshisha University; about the topic on “Nuclear engineering and Society”)
    Prof. Ren Yatsunami (Faculty of Law, Kyushu University; about the topic on “Cultural Heritage and the Metaverse”)[AK2]

    and 1 or 2 speakers.

    14:00–15:30 Group discussion
    15:30–16:00 Summary reports from each group

    Evaluation: Class Participation, Mini-Report & Questionnaire (Submission deadline: June 30th, 2022).


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