Kyushu University [Kishimura Akihiro (Associate Professor) Faculty of Engineering, Department of Applied Chemistry]

Department of Systems Life Sciences
Graduate School of Systems Life Sciences

Department of Materials Science and Engineering
School of Engineering

Office for Promoting Cooperation with Society

Center for Molecular Systems (CMS)

092-802-2851

Ph. D.

No

Polymer Chemistry, Supramolecular Chemistry, Biomaterials, Drug Dleivery System

0000-0002-0503-1418

00years02months

Research Interests

Development of polymeric yolk-shell structure as a protein-concentration capsule and its application for efficient cascade reaction
keyword : enzyme, proteins, polyion complexes, yolk-shell structure, condensed environment, liquid-liquid phase separation, cascade reactions
2020.06.
Establishment of liquid-liquid phase separation model and understanding of function of crowded environments toward artificial cytosol design
keyword : coacervate, liquid-liquid phase separation, proteins
2019.06～2021.03.
TBA
keyword : bioluminescence, nanomedicine, vesicles
2017.04～2019.07.
Establishment of soft material platform based on nano-structured coacervates
keyword : coacervate, block copolymers, polyion complexes, hybrid materials, proteins
2015.04.
TBA
keyword : Drug Delivery System, Nanomedicine, pharmacokinetics, nano-physiology
2018.04～2022.03.
Develpment of novel enzyme-loaded polymeric nano-capsules desinged for in vivo applications
keyword : enzyme, nanoreactors, protein delivery system, nano-medicine, vesicles
2014.04～2017.03.
Development of novel nanoreactor system utilizing molecularly crowded nano-compartments
keyword : Nano-compartment, Vesicles, Molecular Crowding, Enzymatic Reaction
2013.04～2015.03.

Academic Activities

Reports

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1.	An emerging material “PICsome”: A hot zone between “PEG” and “PEG” Recently, nanomaterials constructed by molecular self-assembly have gathered much attention to develop nano-devices incorporated with many types of drugs. Particularly, hollow capsules are one of promising materials, and recently, we have developed polyion complex vesicles, PICsomes, as novel polymeric vesicles. The most advantageous feature of PICsomes is its simple preparation process: Typically, they can be prepared by simple mixing of oppositely charged block copolymers consisting of poly(ethylene glycol) (PEG) and charged poly(amino acid)s in an aqueous medium. Moreover, many other unique properties of PICsomes have been reported, such as facile tuning of vesicle sizes ranging from 100–400 nm while keeping monodispersed size distribution, semipermeable vesicle membrane, facile loading of various water-dispersed materials, long blood circulation after crosslinking, excellent tumor accumulation based on the enhanced permeability and retention (EPR) effect, and so on. The present review article describes basic design and synthetic strategy of PICsomes, fundamental properties of PICsomes, and recent applications of PICsomes to drug delivery system..
2.	Akihiro Kishimura, Horacio Cabral, Kanjiro MIyata, Nanodevices for studying nano-pathophysiology, Advanced Drug Delivery reviews, DOI: 10.1016/j.addr.2014.06.003, 2014.06, Nano-scaled devices are a promising platform for specific detection of pathological targets, facilitating the anal- ysis of biological tissues in real-time, while improving the diagnostic approaches and the efficacy of therapies. Herein, we review nanodevice approaches, including liposomes, nanoparticles and polymeric nanoassemblies, such as polymeric micelles and vesicles, which can precisely control their structure and functions for specifically interacting with cells and tissues. These systems have been successfully used for the selective delivery of reporter and therapeutic agents to specific tissues with controlled cellular and subcellular targeting of biomolecules and programmed operation inside the body, suggesting a high potential for developing the analysis for nano- pathophysiology..
3.	Development and Biomedical Applications of Polymeric Hollow Capsules “PICsomes” Possessing Semipermeable Polyion Complex Membrane.
4.	Akihiro Kishimura, Development of polyion complex vesicles (PICsomes) from block copolymers for biomedical applications, Polymer Journal, 2013.04, [URL], Polyion complex (PIC) formation is one of the most powerful techniques for obtaining molecular self-assemblies in aqueous media. The simple preparation process based on multiple electrostatic interactions is quite attractive for material syntheses, as well as biomedical applications. Therefore, it is desirable to control PIC architectures at the nanoscale in order to expand the scope of PIC materials. In this review article, recent progress on PIC vesicles (PICsomes) is summarized. PICsomes were first developed by my research group, and we recently succeeded in controlling the sizes and structural uniformity of the vesicles. Furthermore, the characteristic dynamic nature of PICs was revealed: PICs were found to exhibit reversible association/ dissociation and structural transformation. We demonstrated that crosslinking the PIC layers of PICsomes is a powerful method for tuning properties such as stability and permeability. Finally, the potential utility of PICsomes for drug delivery nanocarriers was examined, and their future biomedical application is discussed..

Papers

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1.	Beob Soo Kim, Mitsuru Naito, Hiroyuki Chaya, Mao Hori, Kotaro Hayashi, Hyun Su Min, Yu Yi, Hyun Jin Kim, Tetsuya Nagata, Yasutaka Anraku, Akihiro Kishimura, Kazunori Kataoka, and Kanjiro Miyata, Noncovalent Stabilization of Vesicular Polyion Complexes with Chemically Modified/Single-Stranded Oligonucleotides and PEG-b-guanidinylated Polypeptides for Intracavity Encapsulation of Effector Enzymes Aimed at Cooperative Gene Knockdown, Biomacromolecules, https://doi.org/10.1021/acs.biomac.0c01192, 2020.09, For the simultaneous delivery of antisense oligonucleotides and their effector enzymes into cells, nanosized vesicular polyion complexes (PICs) were fabricated from oppositely charged polyion pairs of oligonucleotides and poly(ethylene glycol) (PEG)-b-polypeptides. First, the polyion component structures were carefully designed to facilitate a multimolecular (or secondary) association of unit PICs for noncovalent (or chemical cross-linking-free) stabilization of vesicular PICs. Chemically modified, single-stranded oligonucleotides (SSOs) dramatically stabilized the multimolecular associates under physiological conditions, compared to control SSOs without chemical modifications and duplex oligonucleotides. In addition, a high degree of guanidino groups in the polypeptide segment was also crucial for the high stability of multimolecular associates. Dynamic light scattering and transmission electron microscopy revealed the stabilized multimolecular associates to have a 100 nm sized vesicular architecture with a narrow size distribution. The loading number of SSOs per nanovesicle was determined to be ∼2500 using fluorescence correlation spectroscopic analyses with fluorescently labeled SSOs. Furthermore, the nanovesicle stably encapsulated ribonuclease H (RNase H) as an effector enzyme at ∼10 per nanovesicle through simple vortex-mixing with preformed nanovesicles. Ultimately, the RNase H-encapsulated nanovesicle efficiently delivered SSOs with RNase H into cultured cancer cells, thereby eliciting the significantly higher gene knockdown compared with empty nanovesicles (without RNase H) or a mixture of nanovesicles with RNase H without encapsulation. These results demonstrate the great potential of noncovalently stabilized nanovesicles for the codelivery of two varying bio-macromolecule payloads for ensuring their cooperative biological activity..
2.	Beob Soo Kim, Mitsuru Naito, Rimpei Kamegawa, Hyun Jin Kim, Ryo Iizuka, Takashi Funatsu, Shingo Ueno, Takanori Ichiki, Akihiro Kishimura and Kanjiro Miyata, Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown, CHEMICAL COMMUNICATIONS, 10.1039/d0cc01750g, 56, 66, 9477-9480, 2020.08.
3.	Beob Soo Kim, Sayan Chuanoi, Tomoya Suma, Yasutaka Anraku, Kotaro Hayashi, Mitsuru Naito, Hyun Jin Kim, Ick Chan Kwon, Kanjiro Miyata, Akihiro Kishimura, Kazunori Kataoka, Self-Assembly of siRNA/PEG- b-Catiomer at Integer Molar Ratio into 100 nm-Sized Vesicular Polyion Complexes (siRNAsomes) for RNAi and Codelivery of Cargo Macromolecules, Journal of the American Chemical Society, 10.1021/jacs.8b13641, 141, 8, 3699-3709, 2019.02, Vesicular polyion complexes (PICs) were fabricated through self-assembly of rigid cylindrical molecules, small interfering RNAs (siRNAs), with flexible block catiomers of poly(ethylene glycol) (2 kDa) and cationic polyaspartamide derivative (70 units) bearing a 5-aminopentyl side chain. 100 nm-sized siRNA-assembled vesicular PICs, termed siRNAsomes, were fabricated in specific mixing ranges between siRNA and block catiomer. The siRNAsome membrane was revealed to consist of PIC units fulfilling a simple molar ratio (1:2 or 2:3) of block catiomer and siRNA. These ratios correspond to the minimal integer molar ratio to maximally compensate the charge imbalance of PIC, because the numbers of charges per block catiomer and siRNA are +70 and -40, respectively. Accordingly, the ζ-potentials of siRNAsomes prepared at 1:2 and 2:3 were negative and positive, respectively. Cross-section transmission electron microscopic observation clarified that the membrane thicknesses of 1:2 and 2:3 siRNAsomes were 11.0 and 17.2 nm, respectively. Considering that a calculated long-axial length of siRNA is 5.9 nm, these thickness values correspond to the membrane models of two (11.8 nm) and three (17.7 nm) tandemly aligned siRNAs associating with one and two block catiomers, respectively. For biological application, siRNAsomes were stabilized through membrane-cross-linking with glutaraldehyde. The positively charged and cross-linked siRNAsome facilitated siRNA internalization into cultured cancer cells, eliciting significant gene silencing with negligible cytotoxicity. The siRNAsome stably encapsulated dextran as a model cargo macromolecule in the cavity by simple vortex mixing. Confocal laser scanning microscopic observation displayed that both of the payloads were internalized together into cultured cells. These results demonstrate the potential of siRNAsomes as a versatile platform for codelivery of siRNA with other cargo macromolecules..
4.	Mao Hori, Horacio Cabral, Kazuko Toh, Akihiro Kishimura, Kazunori Kataoka, Robust Polyion Complex Vesicles (PICsomes) under Physiological Conditions Reinforced by Multiple Hydrogen Bond Formation Derived by Guanidinium Groups, Biomacromolecules, 10.1021/acs.biomac.8b01097, 19, 10, 4113-4121, 2018.10, Polyion complex vesicles (PICsomes) formed from a self-assembly of an oppositely charged pair of block- and homo-polyelectrolytes have shown exceptional features for functional loading of bioactive agents. Nevertheless, the stability of PICsomes is often jeopardized in a physiological environment, and only PICsomes having chemically cross-linked membranes have endured in harsh in vivo conditions, such as in the bloodstream. Herein, we developed versatile PICsomes aimed to last in in vivo settings by stabilizing their membrane through a combination of ionic and hydrogen bonding, which is widely found in natural proteins as a salt bridge, by controlled introduction of guanidinium groups in the polycation fraction toward concurrent polyion complexation and hydrogen bonding. The guanidinylated PICsomes were successfully assembled under physiological salt conditions, with precise control of their morphology by tuning the guanidinium content, and the ratio of anionic and cationic components. Guanidinylated PICsomes with 100 nm diameter, which are relevant to nanocarrier development, were stable in high urea concentration, at physiological temperature, and under serum incubation, persisting in blood circulation in vivo..
5.	Masamitsu Suhara, Yutaka Miura, Horacio Cabral, Daisuke Akagi, Yasutaka Anraku, Akihiro Kishimura, Masaya Sano, Takuya Miyazaki, Noriko Nakamura, Ayako Nishiyama, Kazunori Kataoka, Hiroyuki Koyama, Katsuyuki Hoshina, Targeting ability of self-assembled nanomedicines in rat acute limb ischemia model is affected by size, Journal of Controlled Release, 10.1016/j.jconrel.2018.07.049, 286, 394-401, 2018.09, Peripheral artery disease (PAD) is one of the most spreading diseases all over the world. The treatment strategies are limited to surgical or endovascular procedures for final stage chronic PAD or acute limb ischemia, and no pharmacological approaches have been achieved to prevent the worsening of chronic PAD or to regenerate the tissues of acute limb ischemia. Therefore, the improvement of therapeutic strategy is strongly demanded in clinics. Here, we adopted an acute hindlimb ischemia model in rats, which provides concomitant inflammatory response, to evaluate the application of drug delivery system against PAD. Through comparative experiments by using different-sized nanomedicine analogues, polyion complex (PIC) micelles with 30 nm diameter and PIC vesicles with 100- and 200-nm diameter (PICs-30, −100, −200 respectively), we found the size-dependent accumulation and retention in the collateral arteries. In contrast to PICs-30 and -200, histological analysis showed that PICs-100 were around the arterioles and co-localized with macrophages, which indicates that the PICs-100 can achieve moderate interaction with phagocytes. Our data suggests that controlling the size of nanomedicines has promise for developing novel angiogenic treatments toward the effective management of collateral arteries..
6.	Omer F. Mutaf, Yasutaka Anraku, Akihiro Kishimura, Kazunori Kataoka, Unilamellar polyion complex vesicles (PICsomes) with tunable permeabilities for macromolecular solutes with different shapes and sizes, Polymer, 10.1016/j.polymer.2017.10.062, 133, 1-7, 2017.12, Polyion complex vesicles (PICsomes) are characterized by their unique three-layered semipermeable nanomembrane structures, in which a unilamellar PIC layer is sandwiched by poly(ethylene glycol) layers, and have gathered much attention as nano-scaled drug vehicles. Herein, the crosslinking degree of the nanomembrane in the PICsome was controlled systematically for the first time. Permeability of the PICsome nanomembrane was evaluated through a kinetic study of the release of macromolecular cargoes from the PICsome. The degree of crosslinking in the nanomembrane successfully regulated the release behavior. Moreover, the shape and size of the macromolecular solutes were found to be critical factors determining their transport from the inner aqueous phase of the PICsome to the external environment. The results indicate that the unique three-layered structure of PICsome membranes plays a key role in modulating solute transport. These findings will provide a rational strategy for the development of nanomembrane-based controlled-release systems..
7.	Hengmin Tang, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Development of Enzyme Loaded Polyion Complex Vesicle (PICsome): Thermal Stability of Enzyme in PICsome compartment and Effect of Co-Encapsulation of Dextran on Enzyme Activity., Macromolecular Bioscience, 10.1002/mabi.201600542, 2017.05.
8.	Akihiro Kishimura, Akinori Goto, Ping-Shan Lai, Kazunori Kataoka, Facile Preparation of Delivery Platform of Water-Soluble Low-Molecular-Weight Drugs Based on Polyion Complex Vesicle (PICsome) Encapsulating Mesoporous Silica Nanoparticle, ACS Biomaterials Science & Engineering, 10.1021/acsbiomaterials.6b00562, 2017.03.
9.	Akihiro Kishimura, Naoki Sasaki, Kae Sato, A Membrane-Integrated Microfluidic Device to Study Permeation of Nanoparticles through Straight Micropores toward Rational Design of Nanomedicines, Analytical Science, 10.2116/analsci.32.1307, 32, 12, 1307-1314, 2016.12.
10.	Kenshiro Naoyama, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Fabrication of Dendrimer-based Polyion Complex Submicrometer-scaled Structures with Enhanced Stability under Physiological Conditions., Macromolecular Rapid Communications, 10.1002/marc.201600171, 37, 13, 1087-1093, 2016.05.
11.	Akihiro Kishimura, Yutaka Miura, Hiroyuki Koyama, Adequately-Sized Nanocarriers Allow Sustained Targeted Drug Delivery to Neointimal Lesions in Rat Arteries., Molecular Pharmaceutics (ACS), 10.1021/acs.molpharmaceut.6b00219, 13, 6, 2108-2116, 2016.05.
12.	Akihiro Kishimura, Systemically Injectable Enzyme-loaded Polyion Complex Vesicles (PICsomes) as in vivo Nanoreactors Working in Tumor., Angewandte Chemie International Edition, 10.1002/anie.201508339, 55, 560-565, 2016.01, The design and construction of nanoreactors are important for biomedical applications of enzymes, but lipid- and polymeric-vesicle-based nanoreactors have some practical limitations. We have succeeded in preparing enzyme-loaded polyion complex vesicles (PICsomes) through a facile protein- loading method. The preservation of enzyme activity was confirmed even after cross-linking of the PICsomes. The cross- linked b-galactosidase-loaded PICsomes (beta-gal@PICsomes) selectively accumulated in the tumor tissue of mice. Moreover, a model prodrug, HMDER-betaGal, was successfully converted into a highly fluorescent product, HMDER, at the tumor site, even 4days after administration of the beta-gal@PICsomes. Intravital confocal microscopy showed continuous production of HMDER and its distribution throughout the tumor tissues. Thus, enzyme-loaded PICsomes are useful for prodrug activation at the tumor site and could be a versatile platform for enzyme delivery in enzyme prodrug therapy..
13.	Akihiro Kishimura, Induction of Secondary Structure through Micellization of an Oppositely Charged Pair of Homochiral Block- and Homopolypeptides in an Aqueous Medium, Macromol. Rapid Commun, 10.1002/marc.201500368, 2015.08.
14.	Akihiro Kishimura, Density-tunable conjugation of cyclic RGD ligands with polyion complex vesicles for the neovascular imaging of orthotopic glioblastomas, SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS, 10.1088/1468-6996/16/3/035004, 16, 3, 2015.06.
15.	Akihiro Kishimura, Fabrication of polyion complex vesicles with enhanced salt and temperature resistance and their potential applications as enzymatic nanoreactors., American Chemical Society, 10.1021/bm500127g, 15, 7, 2389-2397, 2014, 2014.07.
16.	Akihiro Kishimura, Morphology Control in Water of Polyion Complex Nanoarchitectures of Double-Hydrophilic Charged Block Copolymers through Composition Tuning and Thermal Treatment. , American Chemical Society, 10.1021/ma500314d , 47, 9, 3086-3092, 2014, 2014.04.
17.	Akihiro Kishimura, Polyion complex vesicles for photo-induced intracellular delivery of amphiphilic photosensitizer., J. Am. Chem. Soc., 10.1021/ja406992w, 136, 1, 157-163, 2014, 2013.11.

Presentations

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1.	アウリアファドリナ、森健、片山佳樹、岸村顕広、森本展行、山本雅哉, 経粘膜薬物送達に向けたスルホベタインポリマーの浸透能力評価, 日本薬学会第140年会（京都）, 2020.03.
2.	○MengJu Chan, Kanjiro Miyata, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Facile Fabrication of Silica-Hybrid Polyion Complex Nano-Vesicles and Its Function Enhancement, the 18th Asian Chemical Congres, 2019.12.
3.	○Biplab K C, Takeshi Mori、Akihiro Kishimura、Yoshiki Katayama, Protein Sequestration in Synthetic Di-block-copolymer-based Complex Coacervate by Mimicking Intracellular Phase Separation, the 18th Asian Chemical Congres, 2019.12.
4.	○Biplab K C, Takeshi Mori、Akihiro kishimura、Yoshiki Katayama, Polypeptide based complex coacervate as biomimetic material to sequester biomolecules via rational design of polymeric sidechain, Okinawa Colloids 2019, 2019.11.
5.	○Fadlina Aulia, 松葉弘顕、中瀬生彦、森　健、岸村顕広、片山佳樹, Control of cellular uptake behavior based on tuning of structure and physical properties of PEGylated polyion complex and its application, Okinawa Colloids 2019, 2019.11.
6.	○劉　一イ、森　健、片山佳樹、岸村　顕広, Formation of yolk-shell structure based on self-assembly of polyions and proteins, Okinawa Colloids 2019, 2019.11.
7.	○T. Egashira, T. Mori, Y. Katayama and A. Kishimura, Release of Metal Nanoparticles as Micelles from Complex Coacervates Nano-Architectures, Okinawa Colloids 2019, 2019.11.
8.	○Akihiro Kishimura, Block-copolymer-based polyion complex nanostrucctures as a platform for incorporation of colloidal nanomaterials, Okinawa Colloids 2019, 2019.11.
9.	○Akihiro Kishimura, Control of the Formation Process of Polypeptide Self-assemblies for Understanding Complex Biological Systems:From Nano-physiology to Artificial Cells, Japan-Britain Joint Symposiumu, 2019.09.
10.	○Fadlina Aulia, Morimoto Nobuyuki, Mori Takeshi, Katayama Yoshiki, Kishimura Akihiro, Development of cell penetrating materials for transmucosal drug delivery, 第56回化学関連支部合同九州大会, 2019.07.
11.	○Biplab K C, Takeshi Mori、Akihiro Kishimura、Yoshiki Katayama, Synthetic complex coacervate to sequester functional proteins: A synthetic model for intracellular phase separation, 第19回日本蛋白質科学会年会・第71回日本細胞生物学会大会合同年次大会, 2019.06.
12.	○岸村　顕広, Block-copolymer-based polyion complex nanotechnology as a platform for biomedical appliactions, China-Japan-Singapore Joint Symposium on Supramolecular Systems and Optoelectronic Functions, 2019.06.
13.	○Biplab.K.C, T.Mori, Y.Katayama, A.Kishimura, Introduction of Charge Heterogeneity in Di-block Copolymer for Effective Protein Sequestration in Coacervates, 第24回日本化学会九州支部・韓国化学会釜山支部合同セミナー, 2019.06.
14.	○江頭巧、森健、片山佳樹、岸村顕広, Incorporation of Metal Nanoparticles into Complex Coacervates Nano-Architectures and Their Release via Structural Transformation, 第24回日本化学会九州支部・韓国化学会釜山支部合同セミナー, 2019.06.
15.	○Akihiro Kishimura, Development of biomedical and biomimetic materials utilizing polyion-complex-based nanofabrication techniques, 2019.05.
16.	○Akihiro Kishimura, Development of biomedical and biomimetic materials utilizing polyion-complex-based nanofabrication techniques, 2019.05.
17.	○Akihiro Kishimura, Block-copolymer-based polyion complex nanotechnology as a platform for biomedical applications, 2019.05.
18.	○Akihiro Kishimura, Block-copolymer-based polyion complex nanotechnology as a platform for biomedical applications, 2019.05.
19.	○Y. Liu, T. Mori, Y. Katayama, A. Kishimura, Yolk-Shell assembly formation based on polyion complex of proteins, ACS National Meetin & Expo, 2019.04.
20.	○Y. Liu, T. Mori, Y. Katayama, A. Kishimura, Yolk-Shell assembly formation based on polyion complex of proteins, ACS National Meetin & Expo, 2019.04.
21.	○Akihiro Kishimura, Yiwei Liu, Biplab KC, Takumi Egashira, Takeshi Mori, Yoshiki Katayama, , Block-copolymer-based polyion complexes for utilization of proteins and inorganic nanoparticles, 257th ACS National Meeting, Division of Polymer Chemistry, Polymer-Based Gene & Drug Delivery Systems,, 2019.03.
22.	○B. S. Kim, S. Chuanoi, Y. Anraku, K. Miyata, A. Kishimura, K. Kataoka, , siRNAsome: A self-assembled vesicular architecture formed from siRNAs and PEGylated block catiomers, 6th International Conference on Multifunctional, Hybrid and Nanomaterials 2019,, 2019.03.
23.	○Biplab K C, Takeshi Mori、Akihiro Kishimura、Yoshiki Katayama, Functionalization of polyelectrolyte side chains via chemical modification for effective sequestration of biomolecules into diblock-copolymer-based complex coacervate, 第28回日本MRS年次大会, 2018.12.
24.	○Takumi Egashira・Takeshi Mori・Yoshiki Katayama・Akihiro Kishimura, Thermo-responsive structural transition of nano-structured polyion complexes using, IPC2018, 2018.12.
25.	○松葉弘晃、中瀬生彦、森　健、片山佳樹、岸村　顕広, Utilization of dynamic response of polyion complex for enhancing cell-communication function of nanomedicine, IPC2018, 2018.12.
26.	○Biplab K.C, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Sequestration of biomolecules into diblock-copolymer-based coacervate through chemical modification of polyelectrolyte sidechain, IPC2018, 2018.12.
27.	○B. S. Kim, K. Miyata, A. Kishimura, K. Kataoka, Vesicular self-assemblies from siRNAs and PEGylated block catiomers (siRNAsomes): Their structural, physicochemical, and biological characteristics,, IPC2018, 2018.12.
28.	Akihiro Kishimura, Development of therapeutic nanosystems based on well-designed nano-scaled formulations, 国立中興大学・短期訪問学者セミナー, 2018.10.
29.	Akihiro Kishimura, Polymer nanotechnology for advanced materials: self-assembly and integration of macromolecules and colloidal nanoparticles, 国立中興大学・短期訪問学者セミナー, 2018.10.
30.	Wararu Hatanaka, Hiroki Takeuchi, Akihiro Kishimra, Yoshiki Katayama, ○Takeshi Mori, Modification of Transmembrane Protein Mimics on Living Cells, The 79th Okazaki Conference, 2018.09.
31.	○Akihiro Kishimura, Polymer-nanobiotechnology for Utilization of Proteins Towards Biomedical Application, 第35回国際フォトポリマーコンファレンス　ICPST-35(2018), 2018.06.
32.	○劉　一イ、森　健、片山佳樹、岸村　顕広, 高効率にタンパク質内包が可能なポリイオンコンプレックスyolk-shell構造体の開発, 第34回日本DDS学会学術集会, 2018.06.
33.	○劉　一イ、濱田　祐次朗、森　健、片山佳樹、岸村　顕広, ポリイオンコンプレックス形成に基づくタンパク質内包自己組織化yolk-shell構造の開発, 第67回高分子学会年次大会, 2018.05.
34.	○小川敦嗣、唐蘅敏、森健、片山佳樹、岸村顕広, Development of functionalized PEGylated polymer vesicles for overcoming the mucosal barrier, ISBC2017, 2017.12.
35.	Mikio Terauchi1, ○Biplab KC, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura, Study on functional biomolecule incorporation in complex coacervates using PEG-based block copolymers, ISBC2017, 2017.12.
36.	Akihiro Kishimura, Development of Polyion Complex Vesicle (PICsome) for Biomedical Applications, 理研セミナー, 2017.12.
37.	濱田　祐次朗、尚山　堅士郎、森　健、片山　佳樹、○岸村　顕広, 双親水性ブロック共重合体を用いたいナノ構造化コアセルベートへの機能性ナノ粒子の部位選択的導入, 第27回日本MRS年次大会, 2017.12.
38.	寺内　幹雄、Biplab KC、森　健、片山　佳樹、 ○岸村　顕広, 高分子電解質との複合コアセルベート形成に基づくタンパク質の特異な自己組織化挙動, 第27回日本MRS年次大会, 2017.12.
39.	○濱田祐次朗、檜垣勇次、小椎尾　謙、高原　淳、森　健、片山佳樹、岸村顕広, ブロック共重合体を用いたナノ構造化コアセルベートの設計とミクロな構造とマクロな物性の相関関係の解明, 第27回日本MRS年次大会, 2017.12.
40.	○小川敦嗣、唐蘅敏、森健、片山佳樹、岸村顕広, 粘膜バリア突破を目指した膜機能強化型PEG化ポリマーベシクルの開発, 第27回日本MRS年次大会, 2017.12.
41.	〇松葉弘晃、小川敦嗣、唐蘅敏、山崎北斗、森健、片山佳樹、岸村顕広, 標的組織送達後の機能発現を指向したPEG化ポリイオンコンプレックスナノ粒子の細胞取り込み挙動制御：その粒子形態とPEG鎖長への依存性評価, 第27回日本MRS年次大会, 2017.12.
42.	○松葉弘晃、唐蘅敏、小川敦嗣、森健、片山佳樹、岸村顕広, 標的組織送達後の機能発現に向けたPEG化ポリイオンコンプレックスナノ粒子の細胞取り込み挙動制御：その粒子形態・PEG鎖長依存性, 平成２９年度高分子学会九州支部特別講演会, 2017.11.
43.	○Akihiro Kishimura, Facile Synthesis of Nano-structured Materials Based on Block Copolymer Technology and their Biomedical Applications, 2017International Conference on smart Science, 2017.04.
44.	Akihiro Kishimura, Development of Polymer-Based Supramolecular Nanosystems for Therapeutic Applications, The 50th CMS International Seminar, 2017.03.
45.	○Akihiro Kishimura, Kenshiro Naoyama, Yujiro Hamada, Takeshi Mori, Yoshiki Katayama, Development of nanostructured coacervates based on double hydrophilic block copolymers and the behavior of site-selective incorporation of functional nanoparticles, 日本化学会第97春季年会, 2017.03.
46.	○Yiwei Liu, Hengmin Tang, Takeshi Mori, Yoshiki Katayama and Akihiro Kishimura, Enhanced Protein Encapsulation by Polyion Complex Vesicle Induction on Protein-Polyion Complex Particle, The 11th SPSJ International Polymer Conference (IPC2016), 2016.12.
47.	○Yujiro Hamada, Takeshi Mori, Yoshiki Katayama and Akihiro Kishimura, Design of nano-structured PICs using all-hydrophilic block copolymers and their site-specific incorporation of functional nanomaterials, The 11th SPSJ International Polymer Conference (IPC2016), 2016.12.
48.	○岸村顕広, Development of Self-assembled Nano-structured Materials for Biomedical Applications, MRS-id Meeting 2016, 2016.10.
49.	Akihiro Kishimura, Development of nanostructured soft materials based on all-hydrophilic block copolymers: From basics to biomedical applications,, 2016.10.
50.	○岸村顕広, Development of Novel Supramolecular Hollow Capsules "Picsomes" for Biomedical Applications, ChinaNanomedicine 2016, 2016.10.
51.	○岸村顕広, Development of polyion complex nanostructures based on all-hydrophilic block copolymers, Ostwald Colloquium 2016, 2016.09.
52.	Akihiro Kishimura, Development of polymeric nano-vesicles for advanced drug delivery system, 2016.08.
53.	Akihiro KISHIMURA, Engineering of Enzyme Nano-capsules for Biomedical Applications, CIMTEC2016, 2016.06.
54.	Akihiro Kishimura, Development of polymeric nanomedicine for novel therapy and pathophysiologic study, 2016.05.
55.	Yuki Sakamura , Hengmin Tang , Takeshi Mori , Yoshiki Katayama , ○Akihiro Kishimura, Development of enzyme-loaded polymeric nanocapsules as a versatile platform for enzyme applications: Effect of co-encapsulation of neutral macromolecules, THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015, 2015.12.
56.	○Kenshiro Naoyama, Takeshi Mori , Yoshiki Katayama , Akihiro Kishimura, Site-selective incorporation of nanoparticles into the complex coacervate utilizing self-assembly of block copolymers in aqueous solution, THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015, 2015.12.
57.	○Akihiro Kishimura, Development of polyion complex vesicle “PICsomes” and their unique self-assembling behavior, THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015, 2015.12.
58.	○Hengmin Tang, Yuki Sakamura , Satoshi Tanaka , Takeshi Mori , Yoshiki Katayama , Akihiro Kishimura, Development of a novel enzymatic nano-reactors as biodetoxification nanomedicine, THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015, 2015.12.
59.	Akihiro Kishimura, Rational design of polyion complex nano-architectures for development of functional materials, 2015 Pusan-Gyeongnam/Kyushu-Seibu Joint Symposium on High Polymers (17th) and Fibers (15th), 2015.11.
60.	Akihiro Kishimura, Development of polyion complex nano-vesicles for biomedical applications, Polymers in Medicine and Biology:2015, 2015.09.
61.	○唐　衡敏、森　健、田中　智之、片山佳樹、岸村　顕広, Development of a novel enzymatic nano-reactor for the application of biodetoxification, 42nd CRS Annual Meeting & Exposition, 2015.07.
62.	○唐　衡敏、森　健、田中　智之、片山佳樹、岸村　顕広, Development of enzymatic nano-reactor for removing physiological active substance, 第64回高分子学会年次大会, 2015.05.
63.	Akihiro Kishimura, Development of Polyion Complex Vesicles “PICsomes” with Semipermeable Properties As a Novel Platform for Nano-medicine, The 5th International Conference on the Development of Biomedical Engineering in Vietnam, 2014.06.
64.	Akihiro Kishimura, Novel method to load high amount of drugs and macromolecules into polyion complex vesicles (PICsomes), 40th Annual Meeting & Exposition of the Controlled Release Society, 2013.07.

Membership in Academic Society

Protein Science Society of Japan
Japanese Society for Biomaterials
The Japan Society of Drug Delivery System
Controlled Release Society
American Chemical Society
The Materials Research Society of Japan
The Society for Polymer Science, Japan
The Chemical Society of Japan
The Pharmaceutical Society of Japan

Educational Activities

Classes: Bio-organic chemistry (2013-2015), Biochemistry II (2016-), Molecular Biology (2015-)
Bio-nanotechnology (2014-2016)、Chemistry for Medicine I　(2017-), Chemistry for Medicine II (2017-), Life Engineering I/II

https://kyushu-u.pure.elsevier.com/en/persons/akihiro-kishimura
　Reseacher Profiling Tool　Kyushu University Pure

http://www.chem.kyushu-u.ac.jp/~cms/english/
The web page of the center for molecular systems. .

http://www.chem.kyushu-u.ac.jp/~katayama/en/index.html
Katayama Lab's HP. .

https://kyushu-u.pure.elsevier.com/en/persons/akihiro-kishimura Reseacher Profiling Tool Kyushu University Pure

http://www.chem.kyushu-u.ac.jp/~cms/english/The web page of the center for molecular systems. .

http://www.chem.kyushu-u.ac.jp/~katayama/en/index.htmlKatayama Lab's HP. .

https://kyushu-u.pure.elsevier.com/en/persons/akihiro-kishimura
　Reseacher Profiling Tool　Kyushu University Pure

http://www.chem.kyushu-u.ac.jp/~cms/english/
The web page of the center for molecular systems. .

http://www.chem.kyushu-u.ac.jp/~katayama/en/index.html
Katayama Lab's HP. .