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Junichi Ikenouchi Last modified date：2020.06.17

Professor / Cell biology
Department of Biology
Faculty of Sciences


Graduate School
Department of Systems Life Sciences
Graduate School of Systems Life Sciences
Undergraduate School
Department of Biology
School of Sciences
Administration Post
Other


Homepage
https://kyushu-u.pure.elsevier.com/en/persons/junichi-ikenouchi
　Reseacher Profiling Tool　Kyushu University Pure
http://www.biology.kyushu-u.ac.jp/~taisha/
Ikenouchi Lab HP .
Academic Degree
M.D.Ph.D.
Country of degree conferring institution (Overseas)
No
Field of Specialization
Cell Biology
Total Priod of education and research career in the foreign country
00years00months
Research
Research Interests
  • Elucidation of roles of lipids in the formation of epithelial polarity and cell adhesion
    Elucidation of the organizing principles of living cell membrane by combining synthetic biological approaches and cell biological approaches
    keyword : Epithelial cells, Tight junction, Microvilli, Cell polarity, Biological membrane, Cytoskeleton
    2002.04～2023.09.
Academic Activities
Papers
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1. Shiomi R, Shigetomi K, Inai T, Sakai M, Junichi Ikenouchi, CaMKII regulates the strength of the epithelial barrier, SCIENTIFIC REPORTS, 10.1038/srep13262, 5, 2015.08.
2. 池ノ内 順一, 平田　愛美, 米村　重信, 梅田　真郷, Sphingomyelin clustering is essential for the formation of microvilli., Journal of Cell Science, 10.1242/jcs.122325., 126, 16, 3585-3592, 2013.08, Cellular architectures require regulated mechanisms to correctly localize the appropriate plasma membrane lipids and proteins. Microvilli are dynamic filamentous-actin-based protrusions of the plasma membrane that are found in the apical membrane of epithelial cells. However, it remains poorly understood how their formation is regulated. In the present study, we found that sphingomyelin clustering underlies the formation of microvilli. Clustering of sphingomyelin is required for the co-clustering of the sialomucin membrane protein podocalyxin-1 at microvilli. Podocalyxin-1 recruits ezrin/radixin/moesin (ERM)-binding phosphoprotein-50 (EBP50; also known as NHERF1), which recruits ERM proteins and phosphatidylinositol 4-phosphate 5-kinase β (PIP5Kβ). Thus, clustering of PIP5Kβ leads to local accumulation of phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)P2], which enhances the accumulation of ERM family proteins and induces the formation of microvilli. The present study revealed novel interactions between sphingomyelin and the cytoskeletal proteins from which microvilli are formed, and it clarified the physiological importance of the chemical properties of sphingomyelin that facilitate cluster formation. .
Presentations
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1. 池ノ内順一, Reciprocal Regulation of AJ and TJ During the Assembly of Apical Adhesion Complex, Gordon Research Conference "Cell Contact and Adhesion" 2019, 2019.06, There are many morphologically distinct membrane structures with different functions at the surface of epithelial cells. Among these, adherens junctions (AJ) and tight junctions (TJ) are responsible for the mechanical linkage of epithelial cells and epithelial barrier function, respectively. In the process of new cell–cell adhesion formation between two epithelial cells, such as after wounding, AJ form first and then TJ form on the apical side of AJ. This process is very complicated because AJ formation triggers drastic changes in the organization of actin cytoskeleton, the activity of Rho family of small GTPases, and the lipid composition of the plasma membrane, all of which are required for subsequent TJ formation. In this review, the authors focus on the relationship between AJ and TJ as a representative example of specialization of plasma membrane regions and introduce recent findings on how AJ formation promotes the subsequent formation of TJ..
2. 池ノ内 順一, 塩見　僚, 重富　健太, 上皮間葉転換における細胞膜脂質の質的変化の意義について, BMB2015（第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会）, 2015.12.
Membership in Academic Society
  • The Molecular Biology Society of Japan
  • Japan Society for Cell Biology


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