DOI： https://doi.org/10.1021/acsami.5c02909

記述言語：英語   出版者・発行元：Langmuir  

Tissue engineering and regenerative medicine require biomaterials that balance blood compatibility with cell adhesion, proliferation, and differentiation. Chitosan and its derivatives, owing to their biocompatibility, biodegradability, and functional versatility, have been extensively explored for biomedical applications, including vascular grafts and tissue engineering scaffolds. This study investigates the effect of chemical modifications on the water state of chitosan derivatives─specifically, free water (FW), intermediate water (IW), and nonfreezing water (NFW)─and their implications for protein interactions, platelet adhesion, and mesenchymal stem cell (MSC) behavior. By incorporating hydrophilic and hydrophobic groups, the hydration of chitosan derivatives was precisely controlled, which significantly influenced blood compatibility and cell adhesion. Hexanoyl glycol chitosan (HGC) demonstrated reduced platelet adhesion, low fibrinogen denaturation, and favorable MSC adhesion, making it a promising candidate for applications requiring both enhanced blood compatibility and regenerative potential. These findings underscore the importance of hydration water modulation in designing advanced biomaterials for blood-contacting and regenerative medicine applications.

DOI： 10.1021/acs.langmuir.5c00125

Web of Science

Scopus

PubMed

出版者・発行元：ACS Applied Materials and Interfaces  

We report the development of polyacrylamide hydrogels with photoswitchable stiffness using solely visible light and their application to cell culture. We have previously shown that azobenzenes can control the binding constants of dynamic covalent boronic ester bonds (Chem. Sci. 2018, 9, 5987; J. Am. Chem. Soc. 2020, 142, 19969). Here we show that these photoswitchable dynamic bonds can be incorporated into polyacrylamide hydrogels that are stable for at least 10 days in buffer without changes in stiffness or photoresponse. Reversible stiffening and softening are achieved with green and blue irradiation, respectively. We prepared soft (877 ± 79 Pa) and stiff (8.4 ± 0.3 kPa) hydrogels that undergo rapid, photoreversible changes in modulus over at least 3 light irradiation cycles. In vitro studies show that the hydrogels are nontoxic to HepG2 cells. The cells undergo the expected changes in morphology, actin stress fiber formation, and Yes-associated protein (YAP) subcellular localization upon stiffening and softening the hydrogel substrate with visible light. These results validate the suitability of our visible-light-controlled hydrogel as a versatile platform for cellular mechanotransduction studies.

DOI： 10.1021/acsami.5c02909

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Journal of Korean Medical Science  

Background: In this study, we prepared and evaluated an injectable poloxamer (P407) hydrogel formulation for intratympanic (IT) delivery of dexamethasone (DEX). Methods: DEX-loaded P407 hydrogels were characterized in terms of thermogelation, drug loading capacities, particle size, and drug release. The in vivo toxicity and drug absorption of the DEX-loaded P407 formulation after IT injection were evaluated using an animal model by performing histopathological analysis and drug concentration measurements. Results: The P407 hydrogel effectively solubilized hydrophobic DEX and demonstrated a sustained release compared to the hydrophilic DEX formulation. The in vivo study showed that the hydrogel formulation delivered considerable drug concentrations to the inner ear and displayed a favorable safety profile without apparent cytotoxicity or inflammation. Conclusion: P407 hydrogel can be useful as an injectable inner ear delivery formulation for hydrophobic drugs due to their biocompatibility, drug-solubilizing capacity, thermogelation, and controlled release.

DOI： 10.3346/jkms.2023.38.e135

Scopus

出版者・発行元：Jcis Open  

Metal nanoparticles (NPs), such as gold NPs (AuNPs), are particularly sensitive to X-rays, and thus specific accumulation of AuNPs in a tumor would allow radiotherapy with low energy X-rays and reduced side effects. AuNPs can be generated using HAuCl<inf>4</inf> and the natural polyphenol epigallocatechin-3-gallate (EGCG) in the presence of citrate. Here, we generated EGCG-AuNPs in the presence of several additives and examined the accumulation of these NPs in mouse tumors following intravenous administration. EGCG-AuNPs 15 ​nm in diameter in the presence of sodium alginate accumulated more in tumors compared to 40-nm-diameter EGCG-AuNPs. Furthermore, the results of in vitro cellular uptake and serum protein absorption studies suggest that adsorption of 15–16 ​kDa serum proteins to EGCG-AuNPs suppresses accumulation in tumors. Thus, tendency to adsorb specific proteins on EGCG-AuNPs surface should be tailored for enhancing their accumulation in tumors.

DOI： 10.1016/j.jciso.2023.100074

Scopus

開催年月日： 2023年11月

記述言語：英語  

開催年月日： 2022年7月

記述言語：英語   会議種別：口頭発表（一般）  

国名：アメリカ合衆国  

開催年月日： 2022年4月

記述言語：英語   会議種別：口頭発表（一般）  

国名：アメリカ合衆国  

開催年月日： 2022年3月

記述言語：英語   会議種別：口頭発表（一般）  

国名：アメリカ合衆国  

開催年月日： 2021年4月

記述言語：英語   会議種別：口頭発表（一般）  

国名：アメリカ合衆国  

記述言語：英語   出版者・発行元：日本バイオマテリアル学会  

記述言語：英語   掲載種別：書評論文，書評，文献紹介等  

記述言語：英語   掲載種別：書評論文，書評，文献紹介等  

DOI： https://doi.org/10.1201/9781003169130