Kyushu University Academic Staff Educational and Research Activities Database
List of Reports
Ryuichi Tatsumi Last modified date:2021.07.20

Professor / Animal & Mairne Bioresource Sciences / Department of Bioresource Sciences / Faculty of Agriculture


Reports
1. ABSTRACT:
Muscle fiber type proportions are responsible for a variety of properties of skeletal muscle, including contractile, metabolic, and sensory (differential tasting-component contents and fat deposition); therefore mechanisms that regulate these properties, and their manipulation are hot targets of research for human sports and health sciences and meat-animal production.
We very recently found that resident myogenic stem satellite cells produce and secrete semaphorin 3A (Sema3A) protein exclusively to impact the formation of fatigue-resistant fibers (also called slow-twitch fibers) through a cell-membrane receptor (neuropilin2-plexinA3 complex) → myogenin/MEF2D/HDAC7 → slow-myosin signaling pathway (Tatsumi et al., Stem Cells 35, 1815-1834, 2017). Here we report that an 8-week intake of chlorogenic-acid rich materials (APP; polyphenol mixture prepared from unripe apples) in the diet, may stimulate the Sema3A ligand-dependent pathway concerned. Results demonstrated improvement of lower hind-limb muscle endurance based on increased proportions of fatigue-resistant myofibers (types IIa and I) by the 0.5% APP-feeding to young-adult rats. There was no significant difference in the animal body-phenotypes or locomotor activity shown as total moving distance in light and dark periods, indicating that the shift in myosin heavy chain (MyHC) isoforms from fast-to-slow did not include a bias due to greater exercise behavior by the treated rats. Notably, a subsequent in vitro study showed that supplementation of APP (500 ng/ml) or the major component chlorogenic acid (10 ng/ml) also up-regulated the expression of slow MyHC and the up-stream signaling molecules, myogenin and MEF2D, in primary cultures of differentiating myoblasts. Other major polyphenols found in APP (procyanidin B1, B2, phloridzin, and catechin) in a range of 10-1000 ng/ml did not induce these effects.
Therefore, the present study highlights a possible contribution of dietary chlorogenic acid intake to antagonizing the Sema3A-signaling pathway responsible for fatigue-resistant fiber formation and hence may help in developing a novel strategy for application in human sports and age-related health sciences..
2. Anderson J. E., Do M.-K. Q., Daneshvar N., Suzuki T., Dort J., Mizunoya W., Tatsumi R., The role of semaphorin 3A in myogenic regeneration and the formation of functional neuromuscular junctions on new fibers., Biological Reviews of the Cambridge Philosophical Society, 10.1111/brv.12286, 2017.08.
3. Sang-Gun Roh, Yutaka Suzuki, Takaufmi Gotoh, Ryuichi Tatsumi, Kazuo Katoh, Physiological roles of adipokines, hepatokines, and myokines in ruminants., Asian-Australasian Journal of Animal Sciences,29(1): 1-15, doi.org/10.5713/ajas.16.0001R, 2016.01.
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10. Baba, K., Mizunoya, W., Tatsumi, R., Ikeuchi, Y., and Sekiguchi, T. (2009) Skeletal muscle hypertrophic factors present in the beef extract, The Research Reports of the Ito Foundation, 27, 17-23..
11. Mizunoya, W., Baba, K., Tatsumi, R., Ikeuchi, Y., and Sekiguchi, T. (2008) Skeletal muscle hypertrophic factors present in the beef extract, The Research Reports of the Ito Foundation, 26, 22-27..
12. Mizunoya, W., Iwamoto, Y., Tatsumi, R., Ikeuchi, Y. (2008) The Research Reports of the Uehara Memorial Foundation, Vol. 22.
13. Mizunoya, W., Onuki, Y., Tatsumi, R., and Ikeuchi, Y. (2007) The Research Reports, vol. 15..
14. Tatsumi, R. (2007) Improvement of Age-Related Sarcopenia by Functional Food Ingredients., The Research Reports of the Uehara Memorial Foundation, 21, 92-94..
15. Asuma, A., Mizunoya, W., Tatsumi, R., Ikeuchi, Y., and Sekiguchi, T. (2007) Development of Alkaline Treated Meat Products by High Pressure Technology., The Research Reports of the Ito Foundation, 25, 50-55..
16. Mizunoya, W., Tashiima, A., Tatsumi, R., Ikeuchi, Y. (2007) Screening of Chicken Egg proteins to induce Muscle Hypertrophy, The Research Reports of Kieikai, p.49-54..
17. Ikeuchi, Y., Tatsumi, R., Mizunoya, W. (2006) The Research Reports 2004 of the ALIC, p. 49-62..
18. Tatsumi, R., Shiratsuchi, S., Yamada, M., Mizunoya, W., Ikeuchi, Y., and Sekiguchi, T. (2006) Muscle Hypertrophy and Regeneration Induced by Functional Food Ingredients., The Research Reports of the Ito Foundation, 24, 190-195..
19. Mizunoya, W., Iwamoto, Y., Tatsumi, R., Ikeuchi, Y., and Sekiguchi, T. (2006) The Nutritional Response of Skeletal Muscle Fiber through a Lipid Homeostasis System., The Research Reports of the Ito Foundation, 24, 196-201..
20. Tatsumi, R., Shiratsuchi, S., Katsuki, Y., Haruno, A., Ikeuchi, Y., and Sekiguchi, T. (2005) Studies on Skeletal Muscle Satellite Cell Quiescence In Vitro., The Research Reports of the Ito Foundation 23, 148-153..
21. Tatsumi, R. and Ikeuchi, Y. (2005) More shine to Meat Production: a Programmed Cascade for Muscle Satellite Cell Activation and Quiescence. Proceedings of Japanese Society for Animal Nutrition and Metabolism, 49(1), 1-19..
22. Ikeuchi, Y., Tatsumi, R., Shozaki, y., Takemoto, K., Wakamatsu, J., Haruno, A., Mori, S., Ito, T., and Sekiguchi, T. (2004) Carnitine Supplementation of Rats Decreases Body Fat and Modifies Skeletal Muscle Fiber Types as Does Beef Extract., The Research Reports of the Ito Foundation 22, 238-241..
23. Tatsumi, R., Ikeuchi, Y., Tabata, M., and Tachibana, T. (2004) Molecular Mechanism of Muscle Satellite Cell Activation: Calcium-Calmodulin Dependent Cascade Triggered by Mechanical Stretch., The Research Reports 2003 of the Research Grant from Graduate School of Agriculture, kyushu University, appeared in www.agr.kyushu-u.ac.jp/agr_hp_date/d_nouken/houkokuindex.html..
24. Ikeuchi, Y., Tatsumi, R., Yoshihara, H., Kawabata F., Haruno, A., Mori, S., Ito, T., Hayashi, T., and Sekiguchi, T. (2003) Beef Extract Diet Modifies Skeletal Muscle Fiber Type., The Research Reports of the Ito Foundation 21, 260-264..
25. Tatsumi, R. Sakata, T., Ikeuchi, Y., Ito, T., Allen, R.E., Nishimura, T., and Hattori, A. (2002) Studies on Mechanical Stretch-Induced Activation of Skeletal Muscle Satellite Cells In Vitro., The Research Reports of the Ito Foundation 20, 124-030..
26. Tatsumi, R., Hattori, A., and Ikeuchi, Y. (2002) Molecular Mechanism of Muscle Satellite Cell Activation: Identification of Activation Stimuli in In Vivo Muscle Stretch Model, Research Report of the Grant-in-Aid for Scientific Research (C) (No. 12660299 to R.T.) from the Japan Society for the Promotion of Science..
27. Tatsumi, R. (2001) Molecular Mechanism of Muscle Satellite Cell Activation., Shokuniku-no-Kagaku 42 (2), 123-133..
28. Nishimura, T., Wakamatsu, J.-I., Tatsumi, R. and Hattori, A. (2001) Effect of L-Carnitine on Utilization of Fatty Acid and ATP Production in Cultured Cardiac Myoblasts., The Research Reports of the Ito Foundation 19, 228-232..
29. Ishioroshi, M. and Tatsumi, R. (2000) Meat Quality in "The 45th ICOMST reports, session 5", Meat Journal 3, 94-98..
30. Nishimura, T., Wakamatsu, J.-I., Tatsumi, R. and Hattori, A. (2000) Studies on L-Carnitine Uptake and Proliferation of Myogenic Stem Cells Subjected to Mechanical Stretches., The Research Reports of the Ito Foundation 18, 290-293..
31. Tatsumi, R. (1998) Electrophoresis & Western Blotting in "Methods in Meat and Meat Product Analysis, part 2", Shokuniku-no-Kagaku 39 (2), 7-14..
32. Tatsumi, R. and Allen, R.E. (1998) Studies on Activation of Skeletal Muscle Satellite Cells., The Research Reports of the Ito Foundation 16, 168-172..
33. Tatsumi, R. (1998) Myofibrils in "Methods in Meat and Meat Product Analysis, part 1", Shokuniku-no-Kagaku 39 (1), 42-45..
34. Tatsumi, R. (1997) Activation of Muscle Stem Cells, Kagaku-to-Seibutsu 35(4), 295-297..
35. Hattori, A. and Tatsumi, R. (1989) Improved Detection of Macromolecular Proteins with Sodiumdodecyl Sulfate-Polyacrylamide Slab Gel Electrophoresis, Seikagaku 61(8), 717-719..