| kensuke kusumi | Last modified date：2023.12.06 |
Lecturer /
Informational biology /
Department of Biology /
Faculty of Sciences
Presentations
| 1. | sORF, sorfc03, associated with Nitrogen metabolism in Arabidopsis. |
| 2. | The stringent response regulator ppGpp contributes to the regulation of plastidial GTP pool during chloroplast differentiation in rice. |
| 3. | Arabidopsis short ORF, SORFC03 is involved in the repression of lateral root growth under high-nitrogen conditions. |
| 4. | sORF, sorfc03, associated with Nitrogen metabolism in Arabidopsis. |
| 5. | Kazuhiro Ito, Yuki Hisanaga, Kousuke Hanada, Koh Iba, Kensuke Kusumi, An Arabidopsis short ORF, SORFC03, is involved in the regulation of nitrogen-dependent lateral root developmen, The International Conference on Arabidopsis Research (ICAR), 2021.06, Nitrogen (N) is an important factor for the basis of metabolism for plant growth. While knowledge and understanding of N metabolism has improved considerably over the last several decades, most of the key factors involved in these processes still remain uncharacterized. Recently, short open reading frames (sORFs) were found in the known intergenic regions in eukaryotes. In Arabidopsis, about 8,000 sORFs have already been identified, however, most of them remain uncharacterized. In this study, we characterized one of these sORFs, sorfc03, which is suggested to be involved in the regulation of lateral root (LR) development associated with N metabolism.. |
| 6. | sORFs associated with Nitrogen metabolism in Arabidopsis. |
| 7. | sORFs associated with Nitrogen metabolism in Arabidopsis. |
| 8. | sORFs associated with Nitrogen metabolism in Arabidopsis. |
| 9. | sORFs associated with Nitrogen metabolism in Arabidopsis. |
| 10. | sORFs associated with Nitrogen metabolism in Arabidopsis. |
| 11. | sORFs associated with Nitrogen metabolism in Arabidopsis. |
| 12. | sORFs associated with CO2 adaptation in Arabidopsis. |
| 13. | 楠見 健介, 橋村 綾菜, 射場 厚, Contribution of guard cell SLAC1 anion channel to the growth stage-dependent stomatal regulation in rice , 17th International Congress on Photosynthesis Research, 2016.08, [URL], Rice cultivation mainly depends on soil water availability as well as on carbon fixation by photosynthesis. Therefore, optimal control of stomata, which regulates leaf respiration and CO2 absorption simultaneously, is crucial for healthy growth. SLAC1 is a stomatal anion channel protein controlling stomatal closure in response to environmental [CO2]. SLAC1-deficient mutant of rice, slac1, exhibited significant increase in stomatal conductance (gs) with enhanced CO2 assimilation, showing that this channel plays a major role in stomatal closure in rice. In this study, in order to clarify contribution of the SLAC1 to stomatal regulation, we investigated changes in the stomatal conductance during the vegetative growth stage of rice, and compared them among the wild type (WT), slac1 and SLAC1-F461A, which expressed a dominant point mutation (F461A) causing constitutive stomatal closure. . |
| 14. | SLAC1 is a stomatal anion channel protein controlling stomatal closure. We previously reported that the SLAC1-deficient mutant of rice, slac1, had constitutively higher stomatal conductance compared to wild type. On the other hand, change in the stomatal conductance in response to some environmental cues, such as day-night cycle and change in the external [CO2], still remained in slac1, suggesting the possible existence of SLAC1-independent mechanism regulating stomatal closure. In this study, we examined transgenic rice, SLAC1-F461A, which expressed a point mutation causing an amino acid substitution (F461A) in SLAC1. Because side group of F461 is estimated to function as the channel gate, stomata in the SLAC1-F461A are expected to close constitutively. SLAC1-F461A plant had a high-leaf-temperature phenotype, and measurement of leaf transpiration showed that the stomatal conductance of young leaves of the SLAC1-F461A was about 70% lower than that of wild type. These indicate that in the SLAC1-F461A SLAC1 channels are always open and the stomata close constitutively. We will discuss the effect of the mutation on the growth and photosynthesis and contribution of SLAC1 to stomatal regulation during rice growth.. |
| 15. | Short open reading frames associated with CO2 adaptation in Arabidopsis. |
| 16. | SLAC1 is a stomatal anion channel protein controlling stomatal closure. We previously reported that the SLAC1-deficient mutant of rice, slac1, had constitutively higher stomatal conductance compared to wild type. On the other hand, change in the stomatal conductance in response to some environmental cues, such as day-night cycle and change in the external [CO2], still remained in slac1, suggesting the possible existence of SLAC1-independent mechanism regulating stomatal closure. In this study, we examined transgenic rice, SLAC1-F461A, which expressed a point mutation causing an amino acid substitution (F461A) in SLAC1. Because side group of F461 is estimated to function as the channel gate, stomata in the SLAC1-F461A are expected to close constitutively. SLAC1-F461A plant had a high-leaf-temperature phenotype, and measurement of leaf transpiration showed that the stomatal conductance of young leaves of the SLAC1-F461A was about 70% lower than that of wild type. These indicate that in the SLAC1-F461A SLAC1 channels are always open and the stomata close constitutively. We will discuss the effect of the mutation on the growth and photosynthesis and contribution of SLAC1 to stomatal regulation during rice growth.. |
| 17. | 楠見 健介, 射場 厚, Modulation and maintenance of chloroplast genetic system during early leaf development and at low temperatures in rice, Japanese-Finnish Seminar 2014 "Design of Superior Machinery of Light Energy Conversion in Photosynthetic Organisms", 2014.10, Chloroplast biogenesis is most significant during the changes in cellular organization associated with leaf development in higher plants. During early leaf development, the structure and function of the chloroplasts differ greatly from those in a mature leaf, suggesting the existence of a stage-specific mechanism regulating chloroplast development during this period. We have analyzed genes involved specifically in early chloroplast biogenesis by using low temperature-conditional mutants of rice (Oryza sativa). These genes encode factors involved in chloroplast rRNA regulation (NUS1), and nucleotide metabolism in mitochondria, chloroplasts and cytosol (V2, V3, ST1). These are all preferentially expressed in the early leaf developmental stage P4, and depleting them causes altered chloroplast transcription and translation, and ultimately leaf chlorosis. Accordingly, regulation of cellular nucleotide pools and nucleotide metabolism is likely to be involved in the chloroplast biogenesis under low temperatures at this stage. Current understanding of these factors and organization of them during early leaf development will be discussed.. |
| 18. | The guard cell chloroplast is the site of perception of blue light and photosynthetically active radiation, and of at least one of the mechanisms for sensing CO2 in the guard cell. The guard cell chloroplast has been the focus of intense controversy over its capacity for light sensing and photosynthetic carbon fixation, and the osmoregulatory mechanisms mediating stomatal movements. In this study, gles1 (green less stomata 1), an Arabidopsis mutant with non-chlorophyllous stomata, was isolated. . |
| 19. | SLAC1 is a stomatal anion channel protein controlling stomatal closure in response to environmental [CO2]. Our previous study showed that the SLAC1-deficient mutant of rice, slac1, had higher stomatal conductance, higher rates of photosynthesis and lower leaf temperature compared to the wild-type plants, revealing that SLAC1 was involved in stomatal closure in rice, as was observed in Arabidopsis. On the other hand, change in the stomatal conductance in response to day-night cycle as well as to the external [CO2] still remained in the slac1 mutant, suggesting the possible existence of SLAC1-independent mechanism regulating stomatal closure. To estimate the contribution of SLAC1 to stomatal closure, we examined the effect of different developmental stage and different leaf position on SLAC1 gene expression and the stomatal conductance in the slac1 mutant and wild-type plants. Our data suggest the tissue-specificity and age-dependency of SLAC1 function in the stomatal closure in rice.. |
| 20. | In rice (Oryza sativa L.), leaf photosynthesis is known to be highly correlated with stomatal conductance. Hower, it remains unclear whether stomatal conductance dominantly limits the photosynthetic rate. SLAC1 is a stomatal anion channel protein controlling stomatal closure in response to environmental [CO2]. In order to examine stomatal limitations to photosynthesis, we isolated and characterized a SLAC1-deficient mutant of rice, slac1. The slac1 plant had a constitutive low-leaf-temperature phenotype. Measurement of leaf gas exchange showed that slac1 plants had significantly higher stomatal conductance (gs), rates of photosynthesis (A) and ratios of internal [CO2] to ambient [CO2] (Ci/Ca) compared to wild-type plants, whereas there was no significant difference in the response of photosynthesis to internal [CO2] (A/Ci curves). These observations demonstrate that stomatal conductance is a major determinant of photosynthetic rate in rice.. |
| 21. | Characterization of SLAC1-deficient mutant of rice. |
| 22. | Regulation of chloroplast biogenesis during early stages of leaf development in rice., [URL]. |
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