Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Satoru Kidoaki Last modified date:2020.11.16

Professor / Research Field of Biomedical and Biphysical Chemistry / Department of Applied Molecular Chemistry / Institute for Materials Chemistry and Engineering

1. T. Kuboki, H. Ebata, T. Matsuda, Y. Arai, T. Nagai, S. Kidoaki, Hierarchical development of motile polarity in durotactic cells just crossing an elasticity boundary, Cell Struct. Funct., 45, 33-43, 2020.02.
2. D. Huang and S. Kidoaki, Stiffness-optimized drug-loaded matrix for selective capture and elimination of cancer cells, J. Drug Deliv. Sci. Technol., 55, 10414, 2020.02.
3. Kei Sugihara, Saori Sasaki, Akiyoshi Uemura, Satoru Kidoaki, Takashi Miura, Mechanisms of endothelial cell coverage by pericytes
computational modelling of cell wrapping and in vitro experiments, Journal of the Royal Society, Interface, 10.1098/rsif.2019.0739, 17, 162, 2020.01, Pericytes (PCs) wrap around endothelial cells (ECs) and perform diverse functions in physiological and pathological processes. Although molecular interactions between ECs and PCs have been extensively studied, the morphological processes at the cellular level and their underlying mechanisms have remained elusive. In this study, using a simple cellular Potts model, we explored the mechanisms for EC wrapping by PCs. Based on the observed in vitro cell wrapping in three-dimensional PC-EC coculture, the model identified four putative contributing factors: preferential adhesion of PCs to the extracellular matrix (ECM), strong cell-cell adhesion, PC surface softness and larger PC size. While cell-cell adhesion can contribute to the prevention of cell segregation and the degree of cell wrapping, it cannot determine the orientation of cell wrapping alone. While atomic force microscopy revealed that PCs have a larger Young's modulus than ECs, the experimental analyses supported preferential ECM adhesion and size asymmetry. We also formulated the corresponding energy minimization problem and numerically solved this problem for specific cases. These results give biological insights into the role of PC-ECM adhesion in PC coverage. The modelling framework presented here should also be applicable to other cell wrapping phenomena observed in vivo..
4. Hiroyuki Ebata, Kousuke Moriyama, Thasaneeya Kuboki, Satoru Kidoaki, General cellular durotaxis induced with cell-scale heterogeneity of matrix-elasticity, Biomaterials, 10.1016/j.biomaterials.2019.119647, 230, 2020.02, Stiffness-gradient-induced cellular taxis, so-called durotaxis, has been extensively studied on a substrate with a single broad or steep stiffness gradient. However, in actual living tissues, cells should sense cell-scaled heterogeneous elasticity distribution in the extracellular matrix. In this study, to clarify the effect of the cell-scale heterogeneity of matrix-elasticity on durotaxis, we examined the motility of different types of cells on microelastically-striped patterned gels with different cell-sized widths. We found that cells accumulated in stiff regions with specific width on cell-type-dependency, even when a stiffness gradient is too small to induce usual durotaxis with a monotonic stiffness gradient. Fibroblast cells accumulated in a wide stiff region of multicellular size, while mesenchymal stem cells localized in a narrow stiff region of single-cell size. It was revealed that durotactic activity is critically affected not only with the cell type but also with the cell-scale heterogeneity of matrix-elasticity. Based on the shape-fluctuation-based analysis of cell migration, the dynamics of the pseudopodia were found to play a key role in determining the behaviors of general durotaxis. Our results suggest that design of cell-scale heterogeneity of matrix-elasticity is pivotal in controlling directional cell migration, the spontaneous cell-patterning, and development of the tissue on the biomaterials surfaces..
5. Saori Sasaki, Satoru Kidoaki, Precise design of microwrinkles through the independent regulation of elasticity on the surface and in the bulk of soft hydrogels, Polymer Journal, 10.1038/s41428-019-0299-8, 2019.12, Abstract: It is still difficult to precisely control microscopic wrinkles on the surface of functional materials, especially biomimetic soft hydrogels with an elastic modulus lower than 100 kPa. This is due to the difficulty in realizing the systematic and independent regulation of elasticity on the top surface and in the bulk of hydrogels, which is essential for the generation of surface microwrinkles. To overcome this problem, using a two-step photocrosslinking process with VIS and UV irradiation of a photocurable gelatinous sol, we developed a method for independently regulating the elastic modulus on the surface and in the bulk to obtain wrinkles on a biomimetic soft gel. Photocurable gelatin was first irradiated and crosslinked with VIS light in the presence of the water-soluble radical generator sulfonyl camphorquinone, which is effective in forming thick bulk gels. Next, the top surface of these precrosslinked gels was irradiated with UV light in the presence of surface-coated water-insoluble camphorquinone. As a result, this two-step photocrosslinking process enabled to independently control the elastic moduli of the surface and the bulk lower than 100 kPa and to generate several-micron-scale wrinkles on the soft hydrogel surface..
6. Daoxiang Huang, Yu Nakamura, Aya Ogata, Satoru Kidoaki, Characterization of 3D matrix conditions for cancer cell migration with elasticity/porosity-independent tunable microfiber gels, Polymer Journal, 10.1038/s41428-019-0283-3, 2019.10, The mechanics and architectures of the extracellular matrix (ECM) critically influence 3D cell migration processes, such as cancer cell invasion and metastasis. Understanding the roles of mechanical and structural factors in the ECM could provide an essential basis for cancer treatment. However, it is generally difficult to independently characterize these roles due to the coupled changes in these factors in conventional ECM model systems. In this study, to solve this problem, we developed elasticity/porosity-tunable electrospun fibrous gel matrices composed of photocrosslinked gelatinous microfibers (nanometer-scale-crosslinked chemical gels) with well-regulated bonding (tens-of-micron-scale fiber-bonded gels). This system enables independent modulation of microscopic fiber elasticity and matrix porosity, i.e., the mechanical and structural conditions of the ECM. The elasticity of fibers was tuned with photocrosslinking conditions. The porosity was regulated by changing the degree of interfiber bonding. The influences of these factors of the fibrous gel matrix on the motility of MDA-MB-231 tumorigenic cells and MCF-10A nontumorigenic cells were quantitatively investigated. MDA-MB-231 cells showed the highest degree of MMP-independent invasion into the matrix composed of fibers with a Young’s modulus of 20 kPa and a low degree of interfiber bonding, while MCF-10A cells did not show invasive behavior under the same matrix conditions..
7. Satoru Kidoaki, Frustrated differentiation of mesenchymal stem cells, Biophysical Reviews, 10.1007/s12551-019-00528-z, 11, 3, 377-382, 2019.06, Mesenchymal stem cells (MSCs) are one of the most useful cell resources for clinical application in regenerative medicine. However, standardization and quality assurance of MSCs are still essential problems because the stemness of MSCs depends on such factors as the collection method, individual differences associated with the source, and cell culture history. As such, the establishment of culture techniques which assure the stemness of MSCs is of vital importance. One important factor affecting MSCs during culture is the effect of the mechanobiological memory of cultured MSCs built up by their encounter with particular mechanical properties of the extracellular mechanical milieu. How can we guarantee that MSCs will remain in an undifferentiated state? Procedures capable of eliminating effects related to the history of the mechanical dose for cultured MSCs are required. For this problem, we have tried to establish the design of microelastically patterned cell-culture matrix which can effectively induce mechanical oscillations during the period of nomadic migration of cells among different regions of the matrix. We have previously observed before that the MSCs exposed to such a growth regimen during nomadic culture keep their undifferentiated state—with this maintenance of stemness believed due to lack of a particular regular mechanical dosage that is likely to determine a specific lineage. We have termed this situation as “frustrated differentiation”. In this minireview, I introduce the concept of frustrated differentiation of MSCs and show possibility of purposeful regulation of this phenomenon..
8. Atsushi Sakai, Naomi Hiro-oka, Saori Sasaki, Satoru Kidoaki, Miho Yanagisawa, Lipid membrane effect on the elasticity of gelatin microgel prepared inside lipid microdroplets, Nihon Reoroji Gakkaishi, 10.1678/rheology.47.55, 47, 2, 55-59, 2019.01, In a previous study (Sakai A., et al., ACS Cent. Sci., 4, 477 (2018)), a spherical microgel of gelatin prepared inside a lipid droplet was reported to have a higher surface elasticity than the bulk gel. In this study, we investigate the role of contact or lack of contact between gelatin and the lipid membrane as well as the micrometric confinement to isolate the dominant cause of this higher elasticity of microgels. For our experiment, we prepared a concave microgel of gelatin with two surfaces, with one surface in contact with the lipid membrane and the other without being in contact with the membrane. Next, we measured the elasticities of both the surfaces by using micropipette aspiration. Although the elasticity of the surface not in contact with the lipid membrane was slightly lower than that of the surface in contact with the membrane, the elasticity value was much higher than that for the bulk gel. Further, it was found that the droplet confinement without lipids did not decrease the elasticity of gelatin microgels. These results demonstrate that the dominant factor responsible for the higher elasticity of gelatin microgels is micrometric confinement and not their contact with the lipid membrane..
9. Misato Iwashita, Hatsumi Ohta, Takahiro Fujisawa, Minyoung Cho, Makoto Ikeya, Satoru Kidoaki, Yoichi Kosodo, Brain-stiffness-mimicking tilapia collagen gel promotes the induction of dorsal cortical neurons from human pluripotent stem cells, Scientific reports, 10.1038/s41598-018-38395-5, 9, 1, 2018.12, The mechanical properties of the extracellular microenvironment, including its stiffness, play a crucial role in stem cell fate determination. Although previous studies have demonstrated that the developing brain exhibits spatiotemporal diversity in stiffness, it remains unclear how stiffness regulates stem cell fate towards specific neural lineages. Here, we established a culture substrate that reproduces the stiffness of brain tissue using tilapia collagen for in vitro reconstitution assays. By adding crosslinkers, we obtained gels that are similar in stiffness to living brain tissue (150–1500 Pa). We further examined the capability of the gels serving as a substrate for stem cell culture and the effect of stiffness on neural lineage differentiation using human iPS cells. Surprisingly, exposure to gels with a stiffness of approximately 1500 Pa during the early period of neural induction promoted the production of dorsal cortical neurons. These findings suggest that brain-stiffness-mimicking gel has the potential to determine the terminal neural subtype. Taken together, the crosslinked tilapia collagen gel is expected to be useful in various reconstitution assays that can be used to explore the role of stiffness in neurogenesis and neural functions. The enhanced production of dorsal cortical neurons may also provide considerable advantages for neural regenerative applications..
10. Atsushi Sakai, Yoshihiro Murayama, Kei Fujiwara, Takahiro Fujisawa, Saori Sasaki, Satoru Kidoaki, Miho Yanagisawa, Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space, ACS Central Science, 10.1021/acscentsci.7b00625, 4, 4, 477-483, 2018.04, Even though microgels are used in a wide variety of applications, determining their mechanical properties has been elusive because of the difficulties in analysis. In this study, we investigated the surface elasticity of a spherical microgel of gelatin prepared inside a lipid droplet by using micropipet aspiration. We found that gelation inside a microdroplet covered with lipid membranes increased Young's modulus E toward a plateau value E∗ along with a decrease in gel size. In the case of 5.0 wt % gelatin gelled inside a microsized lipid space, the E∗ for small microgels with R ≤ 50 μm was 10-fold higher (35-39 kPa) than that for the bulk gel (∼3 kPa). Structural analysis using circular dichroism spectroscopy and a fluorescence indicator for ordered beta sheets demonstrated that the smaller microgels contained more beta sheets in the structure than the bulk gel. Our finding indicates that the confinement size of gelling polymers becomes a factor in the variation of elasticity of protein-based microgels via secondary structure changes..
11. H. Ebata, A. Yamamoto, Y. Tsuji, S. Sasaki, K. Moriyama, T. Kuboki, S. Kidoaki, Persistent random deformation model of cells crawling on a gel surface, Sci. Rep., 8, 5153, 2018.03.
12. Kousuke Moriyama, Satoru Kidoaki, Cellular Durotaxis Revisited
Initial-Position-Dependent Determination of the Threshold Stiffness Gradient to Induce Durotaxis, Langmuir, 10.1021/acs.langmuir.8b02529, 2018.01, Directional cell movement from a softer to a stiffer region on a culture substrate with a stiffness gradient, so-called durotaxis, has attracted considerable interest in the field of mechanobiology. Although the strength of a stiffness gradient has been known to influence durotaxis, the precise manipulation of durotactic cells has not been established due to the limited knowledge available on how the threshold stiffness gradient (TG) for durotaxis is determined. In the present study, to clarify the principles for the manipulation of durotaxis, we focused on the absolute stiffness of the soft region and evaluated its effect on the determination of TG required to induce durotaxis. Microelastically patterned gels that differed with respect to both the absolute stiffness of the soft region and the strength of the stiffness gradient were photolithographically prepared using photo-cross-linkable gelatins, and the TG for mesenchymal stem cells (MSCs) was examined systematically for each stiffness value of the soft region. As a result, the TG values for soft regions with stiffnesses of 2.5, 5, and 10 kPa were 0.14, 1.0, and 1.4 kPa/μm, respectively, i.e., TG markedly increased with an increase in the absolute stiffness of the soft region. An analysis of the area and long-axis length for focal adhesions revealed that the adhesivity of MSCs was more stable on a stiffer soft region. These results suggested that the initial location of cells starting durotaxis plays an essential role in determining the TG values and furthermore that the relationship between the position-dependent TG and intrinsic stiffness gradient (IG) of the culture substrate should be carefully reconsidered for inducing durotaxis; IG must be higher than TG (IG ≥ TG). This principle provides a fundamental guide for designing biomaterials to manipulate cellular durotaxis..
13. K. Tamada, E. Usukura, Y. Yanase, A. Ishijima, T. Kuboki, S. Kidoaki, K. Okamoto., LSPR-mediated high axial-resolution fluorescence imaging on a silver nanoparticle sheet, PLOS One, 12, 12, e0189708, 2017.12.
14. S. Masuda, Y. Yanase, E. Usukura, S. Ryuzaki, P. Wang, K. Okamoto, T. Kuboki, S. Kidoaki, and K. Tamada, High-Resolution Imaging of a Cell-Attached Nanointerface Using a Gold-Nanoparticle Two-Dimensional Sheet, Scientific Reports, 7, 3720, 2017.06.
15. Tomo Kurimura, Yoshiko Takenaka, Satoru Kidoaki, Masatoshi Ichikawa, Fabrication of Gold Microwires by Drying Gold Nanorods Suspensions, Adv. Mater. Interf., DOI: 10.1002/admi.201601125, 1601125, 2017.04.
16. Naohiko Shimada, Minako Saito, Sayaka Shukuri, Sotaro Kuroyanagi, Thasaneeya Kuboki, Satoru Kidoaki, Takeharu Nagai, Atsushi Maruyama, Reversible monolayer/spheroid cell culture switching by UCST-type thermoresponsive ureido polymers, ACS Applied Mater. Interf., DOI: 10.1021/acsami.6b07614, 8, 31524-31529, 2016.11.
17. T. Kuboki, S. Kidoaki, Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture, Methods Mol. Biol., DOI 10.1007/978-1-4939-3584-0_25, 1416, 425-441, 2016.04.
18. Fahsai Kantawong, Thasaneeya Kuboki, Satoru Kidoaki, Redox gene expression of adipose-derived stem cells in response to soft hydrogel, Turkish Journal of Biology, 39, 682-691, 2015.06.
19. Ayaka Ueki, Satoru Kidoaki, Manipulation of cell mechanotaxis by designing curvature of the elasticity boundary on hydrogel matrix , Biomaterials, 41, 45-52, 2015, 2014.12.
20. Naohiko Shimada, Satoru Kidoaki, Atsushi Maruyama, Smart hydrogels exhibiting UCST-type volume changes under physiologically relevant conditions , RSC Advances, 4, 52346, 2014, 2014.10.
21. Thasaneeya Kuboki, Wei Chen, Satoru Kidoaki, Time-dependent migratory behaviors in the long-term studies of fibroblast durotaxis on a hydrogel substrate fabricated with a soft band, Langmuir, 30, 6187-6196., 2014.06.
22. Hiroyuki Sakashita, Satoru Kidoaki, Rectified cell migration on saw-like micro-elastically patterned hydrogels with asymmetric gradient ratchet teeth, PLOS One, 8, 10, e78067, 2013.10.
23. Hiroshi Yoshikawa, Takahito Kawano, Takehisa Matsuda, Satoru Kidoaki, Motomu Tanaka, Morphology and adhesion strength of myoblast cells on photocurable gelatin under native and non-native micromechanical environments, J. Phys. Chem. Part B, 117, 4081-4088, 2013.05.
24. Yasuhiro Matsuda, Kazumasa Takatsuji, Yasunori Shimokawa, Moriya Kikuchi, Satoru Kidoaki, Atsushi Takahara, Shigeru Tasaka, Characterization of Complexes Formed by Mixing Aqueous Solutions of Poly(2-Ethyl-2-Oxazoline) and Poly(Methacrylic Acid) with a Wide Range of Concentrations, Polymer, 54, 1896-1904, 2013.03.
25. T. Okuda, Y. Tahara, N. Kamiya, M. Goto, and S. Kidoaki, S/O-nanodispersion electrospun fiber mesh effective for sustained release of healthy plasmid DNA with the structural and functional Integrity, Journal of Biomaterials Science: Polymer Edition, 24, 1277-1290, 2013.01.
26. T. Kuboki, F. Kantawong, R. Burchmore, M.J. Dalby, and S. Kidoaki, 2D-DIGE proteomic analysis of mesenchymal stem cell cultured on the elasticity-tunable hydrogels, Cell Structure and Function, 37, 127-139,2012..
27. S. Shibano, K. Sasaki, S. Kidoaki, T. Iwaki, Detection of Prion Protein Oligomers by Single Molecule Fluorescence Imaging, Neuropathology, 33,1-6, 2012.03.
28. M. Horning, S. Kidoaki, T. Kawano, K. Yoshikawa, Rigidity-matching between cells and the extracellular matrix leads to the stabilization of cardiac conduction, Biophys. J., 102, 379-387, 2012.02.
29. T. Okuda and S. Kidoaki, Multidrug delivery systems with single formulation ~current status and future perspective~, Journal of Biomaterials and Nanobiotechnology, 3, 50-60, 2012.01.
30. N. Sonda, M. Hirano, N. Shimada, A. Kano, S. Kidoaki, A.Maruyama, Cationic Comb-type Copolymers Do Not Cause Collapse but Shrinkage of DNA Molecules, Chem. Lett., 40: 250-251 (2011)., 2011.02.
31. T. Kawano and S. Kidoaki, Elasticity boundary conditions required for cell mechanotaxis on microelastically-patterned gels, Biomaterials, 32: 2725-2733 (2011)., 2011.01, 細胞は弾性基材表面の硬い領域を指向して運動する性質を示す(メカノタクシス)ことが知られていたが、その駆動のための表面弾性勾配の定量的条件は確立されておらず、メカノタクシスを系統的に誘導し制御することは不可能であった。本論文ではこの問題に対して、独自の弾性率可変ヒドロゲルのマイクロ弾性パターニング技術を確立することにより、細胞のメカノタクシスの誘導条件を初めて明確にした。その技術は細胞運動を操作する培養基材設計の一般的な基礎となるものである。.
32. T. Nakagaki, A. Harano, Y. Fuchigami, E. Tanaka, S. Kidoaki, T. Okuda, T. Iwanaga, K. Goto, T. Shinmyuzu, Formation of Nanoporous Fibers by the Self-Assembly of Pyromellitic Diimide-Based Macrocycle, Ang. Chem. Int. Ed., 45: 1-5 (2010)., 2010.11.
33. T. Okuda and S. Kidoaki, Development of time-programmed, dual-release system using multilayered fiber mesh sheet by sequential electrospinning, Journal of Robotics and Mechatronics, 22(5): 4457-4465 (2010)., 2010.05.
34. N. Chen, A. Zinchenko, S. Kidoaki, M. Murata, K. Yoshikawa, Thermo-Switching of the Conformation of Genomic DNA in Solutions of Poly-(N-isopropylacrylamide), Langmuir, 26, 2995-2998 (2010)., 2010.03.
35. T. Okuda, K. Tominaga, S. Kidoaki, Time-programmed dual release formulation by multilayered drug-loaded nanofiber meshes, Journal of Controlled Release, 143, 2, 258-564, 143(2), 258-564 (2010)., 2010.02.
36. F. Ito, K. Usui, D. Kawahara, A. Suenaga, T. Maki, S. Kidoaki, H. Suzuki, M. Taiji, M. Itoh, Y. Hayashizaki, T. Matsuda, Protein-peptide specific interaction-driven hydrogel formation, hydrodynamic shear stress-dependent gel-to-sol reversibility and its potential application to injectable cartilage tissue, Biomaterials, 31, 58-66 (2009)., 2009.09.
37. K. Usui, T. Maki, F. Ito, A. Suenaga, S. Kidoaki, M. Itoh, M. Taiji, T. Matsuda, Y. Hayashizaki, H. Suzuki, Nanoscale elongating control of the self-assembled protein filament with the cysteine-introduced building blocks, Protein Science, 18, 960-969, 18, 960-969 (2009)., 2009.02.
38. S. Kidoaki, T. Matsuda, Vectorial control of cell movement by the design of microelasticity distribution of biomaterial surface, IEEE International Symposium on Micro-NanoMechatronics and Human Science, 469-474 (2008), 2008.11.
39. M. Hirano, N. Shimada, A. Kano, S. Kidoaki, A. Maruyama, Analysis of cationic comb-type copolymers/DNA interaction by the single molecular observation and intermolecular force measurement, Nucleic Acids Symposium Series, 19(1): 61-74, (2008)., 2008.07.
40. S. Kidoaki and T. Matsuda, Microelastic gradient gelatinous gels to induce cellular mechanotaxis, Journal of Biotechnology, 133, 225-230 (2008)., 2008.01.
41. T. Maki, S. Kidoaki, K. Usui, H. Suzuki, M. Ito, F. Ito, Y. Hayashizaki, T. Matsuda, Dynamic force spectroscopy of the specific interaction between PDZ-domain and its recognition peptides, Langmuir, 23, 2668-2673 (2007)., 2007.01.
42. S. Kidoaki and T. Matsuda, Shape-engineered vascular endothelial cells: nitric oxide production, cell elasticity, and actin cytoskeletal features, Journal of Biomedical Materials Research: Part A, 81, 803-810, 81, 803-810 (2007)., 2007.06.
43. S. Kidoaki and T. Matsuda, Shape-engineered fibroblasts: cell elasticity and actin cytoskeletal features characterized by fluorescence and atomic force microscopy, Journal of Biomedical Materials Research: Part A, 81, 728-735, 2007.06.
44. S. Kidoaki and T. Matsuda, Characterization of the cellular biomechanical responses caused on microprocessed substrates: effect of micropatterned cell adhesiveness and microelasticity gradient, , IEEE International Symposium on Micro-NanoMechatronics and Human Science, 5, 63-69, 5, 63-69 (2006)., 2006.11.
45. S. Kidoaki, T. Matsuda, K. Yoshikawa, Relationship between apical membrane elasticity and stress fiber organization in fibroblasts analyzed by fluorescence and atomic force microscopy, Biomechan Model Mechanobiol, 5, 263-272 (2006)., 2006.11.
46. T. Matsuda and S. Kidoaki, Mechanobiology of cell and tissue engineering and multi-scaled process engineering, IEEE International Symposium on Micro-NanoMechatronics and Human Science, 5, 203-205, 5, 203-205 (2005)., 2005.11.
47. K. Usui, S. Katayama, M. Kanamori, C. Kai, M. Okada, J. Kawai, T. Arakawa, P. Carninci, K. Takio, M. Miyano, S. Kidoaki, T. Matsuda, Y. Hayashizaki, H. Suzuki., Protein-protein interactions of the hyperthermophilic archaeon Pyrococcus horikoshii OT3, Genome Biology, 10.1186/gb-2005-6-12-r98, 6, 12, 6, R98 (2005)., 2005.01.
48. A. Idiris, S. Kidoaki, K. Usui, T. Maki, H. Suzuki, M. Ito, M. Aoki, Y. Hayashizaki, T. Matsuda, Force measurement on antigen-antibody interaction by atomic force microscopy using photograft-polymer spacer, Biomacromolecules, 10.1021/bm0502617, 6, 5, 2776-2784, 6, 2776-2784 (2005)., 2005.01.
49. S. Kidoaki, I.K. Kwon, T. Matsuda, Structural feature and mechanical property of in situ-bonded meshes of segmented polyurethane electrospun from mixed solvents, J. Biomed. Mater. Res. B, 10.1002/jbm.b.30336, 76B, 1, 219-229, 76, 219-229 (2005)., 2005.01.
50. A. Takahara, M. Hadano, T. Yamaguchi, H. Otsuka, S. Kidoaki, T. Matsuda, Characterization of novel bio-degradable segmented polyurethanes prepared from amino-acid based diisocyanate, Macromolecular Symp, 10.1002/masy.200550618, 224, 207-217, 224, 207-217 (2005)., 2005.01.
51. T. Matsuda, M. Ihara, H. Inoguchi, I.K. Kwon, K. Takamizawa, S. Kidoaki, Mechano-active scaffold design of small-diameter artificial graft made of electrospun segmented polyurethane mesh fabrics, J. Biomed. Mater. Res. A, 10.1002/jbm.a.30260, 73A, 1, 125-131, 73, 125-131 (2005)., 2005.01.
52. I.K. Kwon, S. Kidoaki, T. Matsuda, Electrospun nano- to microfiber fabrics made of biodegradable copolyesters: structural characteristics, mechanical properties and cell adhesion potential, Biomaterials, 10.1016/j.biomaterials.2004.10.007, 26, 18, 3929-3939, 26(18), 3929-3939 (2005)., 2005.01.
53. S. Ohya, S. Kidoaki, T. Matsuda, Poly(N-isopropylacrylamide) (PNIPAAM)-grafted hydrogel surfaces: Interrelationship between microscopic structures and mechanical property of surface regions and cell adhesiveness, Biomaterials, 10.1016/j.biomaterials.2004.08.006, 26, 16, 3105-3111, 26, 3105-3111 (2005)., 2005.01.
54. S. Kidoaki, I.K. Kwon, T. Matsuda, Mesoscopic spatial designs of nano- and micron-fiber meshes for tissue-engineering matrix and scaffold based on newly devised multilayering and mixing electrospinning techniques, Biomaterials, 10.1016/j.biomaterials.2004.01.063, 26, 1, 37-46, 26(1), 37-46 (2005)., 2005.01.
55. T. Yamaguchi, H. Otsuka, S. Kidoaki, T. Matsuda, A. Takahara, Physicochemical properties and bio-degradation of segmented polyurethane and poly(urethane-urea) derived from lysine-based diisocyanate, Trans. Mater. Res. Soc. Japan, 29 (6), 2873-2876 (2004)., 2004.01.
56. T. Matsuda, I.K. Kwon, S. Kidoaki, Photocurable biodegradable liquid copolymer: synthesis of acrylate-endcapped trimethylene carbonate-based prepolymers, photocuring and hydrolysis, Biomacromolecues, 10.1021/bm034231k, 5, 2, 295-305, 5、295-305 (2004)., 2004.01.
57. T. Iwataki, S. Kidoaki, T. Sakaue, K. Yoshikawa, and S. S. Abramuchuk, Competition Between Compaction of Single Chains and Bundling of Multiple Chains in Giant DNA Molecules, J. Chem. Phys, 120、4004−4011 (2004)., 2004.01.
58. S. Kato, S. Kidoaki, T. Matsuda, Substrate-dependent Cellular Behaviors of Swiss 3T3 Fibroblasts and Activation of Rho Family during Adhesional and Spreading Processes, J. Biomed. Mater. Res, 10.1002/jbm.a.20012, 68A, 2, 314-324, 68, 314-324 (2004)., 2004.01.
59. T. Matsuda, J. Nagase, A. Gouda, Y. Hirano, S. Kidoaki, and Y. Nakayama, Phosphorylcholine-endcapped oligomer and block co-oligomer and surface biological reactivity, Biomaterials, 10.1016/S0142-9612(03)00344-2, 24, 24, 4517-4527, 24, 4517-4527 (2003)., 2003.01.
60. Y. Nakayama, A. Furumoto, S. Kidoaki and T. Matsuda, Photocontrol of Cell Adhesion and Proliferation by a Photoinduced Cationic Polymer Surface, Photochem. Photobiol., 10.1562/0031-8655(2003)077<0480:POCAAP>2.0.CO;2, 77, 5, 480-486, 77(5), 480-486 (2003)., 2003.01.
61. T. Okuda, S. Kidoaki , M. Ohsakia, Y. Koyama, K. Yoshikawa, Time-dependent complex formation of dendritic poly(L-lysine)s with plasmid DNA and correlation with in vitro transfection efficiencies, Org. Biomol. Chem., 1, 1270-1273 (2003)., 2003.01.
62. S.G.Starodoubtsev, S.Kidoaki, K.Yoshikawa, Interaction of Double-stranded T4 DNA with Cationic Gel of Poly(Diallyldimethylammonium Chloride), Biomacromolecules, 10.1021/bm025583e, 4, 1, 32-37, 4, 32-37 (2003)., 2003.01.
63. N. Yoshinaga, K. Yoshikawa, and S. Kidoaki, Multi-scaling in a long semi-flexible polymer chain in 2D, J. Chem. Phys., 10.1063/1.1475759, 116, 22, 9926-9929, 116, 9926-9929 (2002)., 2002.01.
64. S. Kidoaki, S. Ohya, Y. Nakayama, and T. Matsuda, Thermo-Responsive Property of N-isopropylacrylmide Graft-Polymerized Surfaces Measured with an Atomic Force Microscope, Langmuir, 17, 2402-2407 (2001)., 2001.01.
65. S. Kidoaki, Y. Nakayama, and T. Matsuda, Measuerment of Interaction Forces Between Proteins and Iniferter-Based Graft-Polymerized Surfaces with an Atomic Force Microscope in an Aqueous Media, Langmuir, 17, 1080-1087 (2001)., 2001.01.
66. T. Iwataki, Y. Yoshikawa, S. Kidoaki, D. Umeno, M. Kiji, M. Maeda, Cooperativity vs. Phase Transition in a Giant Single DNA Molecules, J. Am. Chem. Soc, 10.1021/ja000230d, 122, 41, 9891-9896, 122, 9891-9896 (2000)., 2000.01.
67. S. Kidoaki and T. Matsuda, Adhesion Forces of the Blood Plasma Proteins on Self-Assembled Monolayer Surfaces of Alkanethiolates with Different Functional Groups Measured by an Atomic Force Microscope, Langmuir, 15, 7639-7646 (1999)., 1999.01.
68. S. Kidoaki and K. Yoshikawa, Folding and Unfolding of a Giant Duplex-DNA in a Mixed Solution with Polycations, Polyanions, and Crowding Neutral Polymers, Biophys. Chem, 76, 133-143 (1999)., 1999.01.
69. D. Umeno, M. Maeda, S. Kidoaki, and K. Yoshikawa, Temperature-directed compaction of single DNA molecule grafted with poly(N-isopropylacrylamide), Nucleic Acids Res. Symp. Ser., 39, 175-176 (1998)., 1998.01.
70. N. Emi, S. Kidoaki, K. Yoshikawa and H. Saito, Gene Delivery Mediated by Polyarginine Requires a Formation of Big Carrier-Complex of DNA Aggregate, Biochem. Biophys. Res. Commun, 231, 421-424 (1997)., 1997.01.
71. S. Kidoaki and K. Yoshikawa, Controlling the Folding Transition of Giant DNA: Cooperative Effect Between Neutral Polymer and Basic polypeptide, Nucleic Acids Res. Symp. Ser, 35, 115-116 (1996)., 1996.01.
72. V. V. Vasilevskaya, A. R. Khokhlov, S. Kidoaki and K. Yoshikawa, Structure of Collapsed Persistent Macromolecule: Toroid vs. Spherical Globule, Biopolymers, 41, 51-60 (1997)., 1997.01.
73. H. Noguchi, S. Saito, S. Kidoaki and K. Yoshikawa, Self Organized Nanostructure Constructed with a Single Polymer Chain., Chem. Phys. Lett, 261, 527-533 (1996)., 1996.01.
74. K. Yoshikawa, S. Kidoaki, M. Takahashi, V. V. Vasilevskaya and A. R. Khokhlov, Marked Discreteness on The Coil-Globule Transition of Single Duplex-DNA, Ber. Bunsen-Ges. Phys. Chem, 100, 876-880 (1996)., 1996.01.
75. S. Kidoaki and K. Yoshikawa, The Folded State of Long Duplex-DNA Chain Reflects Its Solution History, Biophys. J., 71, 932-939 (1996)., 1996.01.
76. T. Imae and S. Kidoaki, Solution Properties of Fibrous Chains Constructed of Amphiphilic Molecules, J. Jpn. Oil Chem. Soc. (YUKAGAKU), 44, 301-308 (1996)., 1995.01.
77. S. Kidoaki and K. Yoshikawa, The Multistability Observed on the Condensed Structure of DNA/Cationic Polypeptide Complex, Nucleic Acids Res. Symp. Ser, 31, 183-184 (1994)., 1994.01.