Kyushu University Academic Staff Educational and Research Activities Database
List of Reports
Hidefumi Maeda Last modified date:2022.06.23

Professor / Oral Rehabilitation / Department of Dental Science / Faculty of Dental Science

1. Maeda H, Aging and senescence of dental pulp and hard tissues of the tooth., Front Cell Dev Biol., 10.3389/fcell.2020.605996, 2020.11.
2. Maeda H, Mass acquisition of human periodontal ligament stem cells., World J Stem Cells., 10.4252/wjsc.v12.i9.1023, 2020.09, The periodontal ligament (PDL) is an essential fibrous tissue for tooth retention in the alveolar bone socket. PDL tissue further functions to cushion occlusal force, maintain alveolar bone height, allow orthodontic tooth movement, and connect tooth roots with bone. Severe periodontitis, deep caries, and trauma cause irreversible damage to this tissue, eventually leading to tooth loss through the destruction of tooth retention. Many patients suffer from these diseases worldwide, and its prevalence increases with age. To address this issue, regenerative medicine for damaged PDL tissue as well as the surrounding tissues has been extensively investigated regarding the potential and effectiveness of stem cells, scaffolds, and cytokines as well as their combined applications. In particular, PDL stem cells (PDLSCs) have been well studied. In this review, I discuss comprehensive studies on PDLSCs performed in vivo and contemporary reports focusing on the acquisition of large numbers of PDLSCs for therapeutic applications because of the very small number of PDLSCs available in vivo. .
3. Yoshida S, Tomokiyo A, Hasegawa D, Hamano S, Sugii H, Maeda H., Insight into the Role of Dental Pulp Stem Cells in Regenerative Therapy., Biology (Basel), 10.3390/biology9070160., 2020.07.
4. @Hosoya N, @Takigawa T, @Horie T, Maeda H, @Yamamoto Y, @Momoi Y, @Yamamoto K, @Okiji T., A review of the literature on the efficacy of mineral trioxide aggregate in conservative dentistry., Dent Mater J., 2019.10.
5. Tomokiyo A, Wada N, Maeda H., Periodontal Ligament Stem Cells: Regenerative Potency in Periodontium. , Stem Cells Dev., 2019.08.
6. Tomokiyo A, Yoshida S, Hamano S, Hasegawa D, Sugii H, Maeda H., Detection, Characterization, and Clinical Application of Mesenchymal Stem Cells in Periodontal Ligament Tissue., Stem Cells Int., 2018.08.
7. Tomokiyo A, Hamano S, Hasegawa D, Sugii S, Yoshida S, Maeda H. , Prospects for the Application of Neural Crest Cells for the Periodontal Therapy., J Dent Oral Biol. , 2017.09.
8. Atsushi Tomokiyo, Naohisa Wada, Hidefumi Maeda, Contribution of stem cells to dental tissue regeneration; isolation, function, and application., Frontiers in Stem Cell and Regenerative Medicine Research Vol. 2, 2016, 3-38, 2016.07.
9. Tomokiyo A, Wada N, Hamano S, Hasegawa D, Sugii H, Yoshida S, Maeda H. , Periodontal Ligament Stem Cells in Regenerative Dentistry for Periodontal Tissues. , J Stem Cell Res Ther, 2016.07.
10. Naohisa Wada, Atsushi Tomokiyo, Hidefumi Maeda, Future Perspectives in Dental Stem Cell Engineering and the Ethical Considerations. , Dental Stem Cells. , 2016.06.
11. Hidefumi Maeda, Atsushi Tomokiyo, naohisa wada, Koori Katsuaki, Giichiro Kawachi, Akifumi Akamine, Regeneration of the periodontium for preservation of the damaged tooth, Hindawi Publishing Corporation, 2014.10, The population of the world grows every year, and life expectancy tends to increase. Thus, longterm preservation of teeth in aged individuals is an urgent issue. The main causes of tooth loss are well known to be periodontitis, caries, fractures, and orthodontic conditions. Although implant placement is a widely accepted treatment for tooth loss, most patients desire to preserve their own teeth. Many clinicians and researchers are therefore challenged to treat and preserve
teeth that are irreversibly affected by deep caries, periodontitis, fractures, and trauma. Tissue engineering techniques are beneficial in addressing this issue; stem
cells, signal molecules, and scaffolds are the main elements of such techniques. In this review, we describe these three elements with respect to their validation for regeneration of the periodontium and focus particularly on the potency of diverse scaffolds. In addition, we provide a short overview of the ongoing studies of 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl-borane resin including calcium chloride or hydroxyapatite for periodontium regeneration..
12. Hidefumi Maeda, Akifumi Akamine, Quest for the development of tooth root/periodontal ligament complex by tissue engineering. , Integr Mol Med. , 1(2): 22-25, 2014. Doi: 10.15761/IMM.1000106, 2014.10, The life-span of the tooth is intimately-associated with healthiness of periodontal ligament (PDL) which is a connective tissue situated between bone and cementum that covers tooth root surface. However, once this tissue is severely damaged by deep caries, periodontitis, and trauma, this leads to severe difficulty in its regeneration, resulting in tooth loss and decreased quality of life. The development of the therapy for generation and regeneration of the periodontal tissue is an urgent issue. Therefore, researchers have tried to improve efficiently-generative and regenerative medicine using stem cells, signal molecules, and scaffolds, requisite for tissue regeneration. In recent studies, a dental follicle tissue that is composed of stem cell population potentially differentiating into PDL tissue, cementum, and alveolar bone is of current interest. More recently a revolutionary and attractive study reporting the development of bio-hybrid implant that reserved newly-formed cementum/PDL tissue complex on its surface was introduced. In this review, we describe comprehensive reports that tried to develop the cementum/PDL complex by tissue engineering and future prospects..
13. Hidefumi Maeda, naohisa wada, Atsushi Tomokiyo, Monnouchi Satoshi, Akifumi Akamine, Prospective Potency of TGF-1 on Maintenance and Regeneration of Periodontal Tissue., Elsevier Adadmic Press, 2013.06.
14. Hidefumi Maeda, Shinsuke Fujii, Atsushi Tomokiyo, Naohisa Wada and Akifumi Akamine, Potentials of periodontal ligament stem/progenitor cell lines in regeneration studies, Quintessence, 2011.12.
15. Hidefumi Maeda, Atsushi Tomokiyo, Shinsuke Fujii, Naohisa Wada and Akifumi Akamine, Promise of periodontal ligament stem cells, BioMed Central, 2011.07.
16. G Lu, H Maeda, SV Reddy, N Kurihara, GD Roodman, Cloning and characterization of the annexin II receptor., JOURNAL OF BONE AND MINERAL RESEARCH, Vol.20, No.9, p.S83, 2005.09.
17. H Maeda, SV Reddy, N Kurihara, GD Roodman, Characterization and cloning of the Annexin II receptor., JOURNAL OF BONE AND MINERAL RESEARCH, Vol.16, p.S381, 2001.09.
18. N Kurihara, SV Reddy, H Maeda, GD Roodman, Measles virus preferentially induces. vitamin D receptor gene activation in pagetic osteoclast precursors., JOURNAL OF BONE AND MINERAL RESEARCH, Vol.16, p.S182, 2001.09.
19. M Koide, N Kurihara, H Maeda, SV Reddy, Osteoclast inhibitory peptide-1/hSca mediates interferon-gamma inhibition of osteoclast formation., JOURNAL OF BONE AND MINERAL RESEARCH, Vol.16, p.S501, 2001.09.
20. H Maeda, SV Reddy, N Kurihara, GD Roodman, Characterization of the annexin II receptor on marrow stromal cells., JOURNAL OF BONE AND MINERAL RESEARCH, Vol.15, p.S387, 2000.09.
21. N Kurihara, M Koide, A Mansell, H Maeda, RJ Leach, SV Reddy, Mapping and characterization of the functional domain of osteoclast inhibitory peptide-1/hSca., JOURNAL OF BONE AND MINERAL RESEARCH, Vol.15, p.S515, 2000.09.
22. A Kukita, T Kukita, M Ouchida, H Maeda, H Yatsuki, O Kohashi, Osteoclast-derived zinc finger (OCZF) protein with POZ domain, a possible transcriptional repressor, is involved in osteoclastogenesis, BLOOD, Vol.94, No.6, pp.1987-1997, 1999.09, The differentiation of osteoclasts is regulated by transcription factors expressed in cells of osteoclast lineage. We isolated here a potential transcription factor from a cDNA library of an enriched population of preosteoclasts and osteoclasts, The cDNA encodes a protein with hi-terminal POZ domain and C-terminal Kruppel-like zinc fingers, We designate this protein as osteoclast-derived zinc finger (OCZF), OCZF was found to be rat homologue of mouse leukemia/lymphoma-related factor (LRF), Northern blot and in situ hybridization analysis showed OCZF mRNA at a high level in osteoclasts and kidney cells. OCZF had a nuclear targeting sequence and was localized in the nucleus of transfected cells. In addition, OCZF specifically bound to the guanine-rich consensus sequences of Egr-1 and c-Krox. Transient transfection assays indicate that OCZF can repress transcription activity like other POZ domain proteins. Furthermore, antisense but not sense phosphorothioate oligodeoxynucleotides (ODNs) for OCZF cDNA suppressed the formation of osteoclast-like multinucleated cells (MNCs) in bone marrow culture, whereas the same ODNs did not significantly affect the formation of macrophage polykaryons and mononuclear preosteoclast-like cells (POCs), These results suggest that OCZF is a unique transcription factor that plays an important role in the late stage of osteoclastogenesis, (C) 1999 by The American Society of Hematology..
23. T Tsukuba, H Sakai, M Yamada, H Maeda, H Hori, T Azuma, A Akamine, K Yamamoto, Biochemical properties of the monomeric mutant of human cathepsin E expressed in Chinese hamster ovary cells: Comparison with dimeric forms of the natural and recombinant cathepsin E, JOURNAL OF BIOCHEMISTRY, Vol.119, No.1, pp.126-134, 1996.01, Cathepsin E (CE) is the only known aspartic proteinase that exists as a homodimer consisting of two fully catalytically active monomers, which are covalently bound by a disulfide bond between two cysteine residues at the NH2-terminal region (Cys(43) in human pro-CE). To understand the physiological significance of the dimer formation, the monomeric mutant of human CE was constructed by site-directed mutagenesis (Cys(43)-->Ser(43)) and expressed in Chinese hamster ovary (CHO) cells. Immunolocalization of the mutant protein at both the light and electron microscopic levels revealed the monomeric CE to be associated predominantly with the endoplasmic reticulum and the non-lysosomal endocytic organelles. The cellular localization of the monomeric protein was compatible with that of the wild-type (dimeric form) of recombinant human CE expressed in the same cells. The monomeric protein was generated primarily as the 46-kDa pro-CE with a high-mannose-type oligosaccharide chain in the cells. In addition to the maximal activation at around pH 3.5, a substantial proportion of the monomeric pro-CE was converted to the mature form by incubation at pH7 and 37 degrees C for 5 min. In contrast, the dimeric pro-CE was scarcely activated by treatment at pH7. Although catalytic properties of the in vitro-activated monomeric CE appeared to be indistinguishable from those of the dimeric forms of natural and recombinant CE, the monomeric form was more unstable to pH and temperature changes than these dimeric forms. These results indicate that the dimerization of CE is not necessarily required for proper folding to express activity, correct intracellular localization and carbohydrate modification, but that it may be essential to structurally stabilize the molecule in vivo..