Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
YUKIKO OHYAMA Last modified date:2021.06.28

Lecturer / Maxillofacial Diagnostic and Surgical Sciences / Maxill Ofacial Surgery / Kyushu University Hospital

1. Azusa Suzuki, Goro Sugiyama, Yukiko Ohyama, Wataru Kumamaru, Tomohiro Yamada, Yoshihide Mori, Regulation of NF-kB signalling through the PR55β-RelA interaction in osteoblasts, In Vivo, 10.21873/invivo.11813, 34, 2, 601-608, 2020.01, Background/Aim: Nuclear factor kappa B (NFkB) signalling including the RelA subunit is activated upon fibroblast growth factor (FGF) stimulation. A clear understanding of the mechanisms underlying this action will provide insights into molecular targeting therapy. Furthermore, protein phosphatase 2A (PP2A) is involved in RelA dephosphorylation, but little is known about the underlying mechanism. Materials and Methods: Because the regulatory subunits of PP2A drive NF-kB signalling via RelA, we used qRT-PCR and immunoblot analysis to investigate the expression of these subunits in MC3T3-E1 cells. We examined weather FGF2 interacts with NF-kB using immunocytochemistry (IC), immunoprecipitation (IP), and pull-down assay (PD) using recombinant proteins. Results: PR55β expression was increased, whereas activated RelA was dephosphorylated upon FGF2 stimulation. Further, the interaction of PR55β with RelA was confirmed by IC, IP, and PD. Conclusion: FGF2-induced PR55β directly interacts with RelA and regulates NF-kB signalling..
2. Mizuki Sakamoto, Masafumi Moriyama, Mayumi Shimizu, Akira Chinju, Keita Mochizuki, Ryusuke Munemura, Keiko Ohyama, takashi maehara, Kenichi Ogata, Miho Ohta, Masaki Yamauchi, Noriko Ishiguro, Mayu Matsumura, Yukiko Ohyama, Tamotsu Kiyoshima, Seiji Nakamura, The diagnostic utility of submandibular gland sonography and labial salivary gland biopsy in IgG4-related dacryoadenitis and sialadenitis
Its potential application to the diagnostic criteria, Modern Rheumatology, 10.1080/14397595.2019.1576271, 2019.01, Objectives: In this study, we investigated the diagnostic utility of submandibular gland (SMG) sonography and labial salivary gland (LSG) biopsy as a less invasive procedure for diagnosing IgG4-related dacryoadenitis and sialadenitis (IgG4-DS) Methods: Sixty-eight patients with suspected IgG4-DS by presenting swelling of elevated serum IgG (>1747 mg/dl) and/or swelling glands underwent SMG sonography, LSG biopsy and measurement for serum IgG4. SMG sonographic diagnosis was determined by the following characteristic changes; ‘hypoechoic areas of a nodal pattern with high vascularity’ and/or ‘hypoechoic areas of a reticular pattern in the superficial part’. Results: Thirty-one patients were diagnosed with IgG4-DS, 5 with IgG4-RD unaccompanied by lacrimal and salivary gland lesions, 28 with Sjögren’s syndrome, and 4 with malignant lymphoma. The sensitivity, specificity, and accuracy of SMG sonography and LSG biopsy were 100%, 83.8%, 91.2% and 64.5%, 73.8%, 75.0%, respectively. Moreover, those of SMG sonography and LSG biopsy combined with serum IgG4 concentration (>135 mg/dl) were 100%, 94.6%, 97.1% and 64.5%, 91.9%, 79.4%, respectively. Conclusion: LSG biopsy needs to be extremely careful to diagnose IgG4-DS because of its low sensitivity. SMG sonography is sufficient for the diagnosis of IgG4-DS, especially when combined with serologic analysis. Thus, SMG sonography could adapt to the diagnostic criteria of IgG4-DS as a non-invasive method..
3. Reiko U. Yoshimoto, Reona Aijima, Yukiko Ohyama, Junko Yoshizumi, Tomoko Kitsuki, Yasuyoshi Ohsaki, Ai Lin Cao, Atsushi Danjo, Yoshio Yamashita, Tamotsu Kiyoshima, Mizuho A. Kido, Impaired Junctions and Invaded Macrophages in Oral Epithelia With Oral Pain, Journal of Histochemistry and Cytochemistry, 10.1369/0022155418812405, 67, 4, 245-256, 2019.04, Recurrent or chronic oral pain is a great burden for patients. Recently, the links between epithelial barrier loss and disease were extended to include initiation and propagation. To explore the effects of pathohistological changes in oral epithelia on pain, we utilized labial mucosa samples in diagnostic labial gland biopsies from patients with suspected Sjögren’s syndrome (SS), because they frequently experience pain and discomfort. In most labial mucosa samples from patients diagnosed with SS, disseminated epithelial cellular edema was prevalent as ballooning degeneration. The disrupted epithelia contained larger numbers of infiltrating macrophages in patients with oral pain than in patients without pain. Immunohistochemistry revealed that edematous areas were distinct from normal areas, with disarranged cell–cell adhesion molecules (filamentous actin, E-cadherin, β-catenin). Furthermore, edematous areas were devoid of immunostaining for transient receptor potential channel vanilloid 4 (TRPV4), a key molecule in adherens junctions. In an investigation on whether impaired TRPV4 affect cell–cell adhesion, calcium stimulation induced intimate cell–cell contacts among oral epithelial cells from wild-type mice, while intercellular spaces were apparent in cells from TRPV4-knockout mice. The present findings highlight the relationship between macrophages and epithelia in oral pain processing, and identify TRPV4-mediated cell–cell contacts as a possible target for pain treatment..
4. Kana Ishibashi, kotaro ishii, Goro Sugiyama, Yu Kamata, Azusa Suzuki, Kumamaru Wataru, Yukiko Ohyama, Hiroyuki Nakano, Tamotsu Kiyoshima, Tomoki Sumida, Tomohiro Yamada, Yoshihide Mori, Regulation of β-catenin phosphorylation by PR55β in adenoid cystic carcinoma, Cancer Genomics and Proteomics, 10.21873/cgp.20064, 15, 1, 53-60, 2018.01, Background/Aim: Adenoid cystic carcinoma (AdCC) is a rare cancer of the salivary gland with high risk of recurrence and metastasis. Wnt signalling is critical for determining tumor grade in AdCC, as it regulates invasion and migration. β-catenin dephosphorylation plays an important role in the Wnt pathway, but its underlying molecular mechanism remains unclear. Materials and Methods: Because the regulatory subunits of protein phosphatase 2A (PP2A) drive Wnt signalling via target molecules, including β-catenin, we used qRT-PCR and immunoblot analysis to investigate the expression of these subunits in an AdCC cell line (ACCS) and a more aggressive subline (ACCS-M). Results: PR55β was highly expressed in ACCS-M, suggesting its functional importance. In addition, PR55β expression was associated with tumor grade, with ACCS-M exhibiting higher PR55β levels. More importantly, knockdown of PR55β in ACCS-M cells significantly reduced invasiveness and metastatic ability. Furthermore, dephosphorylation and total levels of β-catenin were dependent on PR55β in ACCS-M. Finally, we confirmed a correlation between PR55β staining intensity and histopathological type in human AdCC tissues. Conclusion: Our study provides new insight into the interaction between PR55β and β-catenin and suggests that PR55β may be a target for the clinical treatment of AdCC..
5. Minami Shibuya, Tatsuya Ikari, Goro Sugiyama, Yukiko Ohyama, Kumamaru Wataru, Koki Nagano, Tsuyoshi Sugiura, Kanemitsu Shirasuna, Yoshihide Mori, Efficient regulation of branching morphogenesis via fibroblast growth factor receptor 2c in early-stage embryonic mouse salivary glands, Differentiation, 10.1016/j.diff.2016.05.005, 92, 4, 216-224, 2016.10, Salivary gland (SG) defects have a wide range of health implications, including xerostomia, bacterial infections, and oral health issues. Branching morphogenesis is critical for SG development. A clear understanding of the mechanisms underlying this process will accelerate SG regeneration studies. Fibroblast growth factor receptor 2 (FGFR2) interacts with multiple fibroblast growth factors (FGFs), which promote development. FGFR2 consists of two isoforms, FGFR2b and FGFR2c. FGFR2b is critical for SG development, but little is known about the expression and function of FGFR2c. We investigated the expression of all FGFR family members in fetal SGs between embryonic day 12.5 (E12.5) and E18.5. Based on RT-PCR, we observed an increase in the expression of not only Fgfr2b, but also Fgfr2c in early-stage embryonic mouse SGs, suggesting that FGFR2c is related to SG development. The branch number decreased in response to exogenous FGF2 stimulation, and this effect was suppressed by a mouse anti-FGFR2c neutralizing antibody (NA) and siRNA targeting FGFR2c, whereas FGFR2b signaling was not inhibited. Moreover, the expression of marker genes related to EMT was induced by FGF2, and this expression was suppressed by the NA. These results suggested that branching morphogenesis in SGs is regulated by FGFR2c, in addition to FGFR2b. Interestingly, FGFR2c signaling also led to increased fgf10 expression, and this increase was suppressed by the NA. FGFR2c signaling regulates branching morphogenesis through the activation of FGFR2b signaling via increased FGF10 autocrine. These results provide new insight into the mechanisms by which crosstalk between FGFR2b and FGFR2c results in efficient branching morphogenesis..
6. Kouhei Hayashi, Tatsuya Ikari, Goro Sugiyama, Tsuyoshi Sugiura, Yukiko Ohyama, Kumamaru Wataru, Kanemitsu Shirasuna, Yoshihide Mori, Involvement of the T-box transcription factor Brachyury in early-stage embryonic mouse salivary gland, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2016.06.140, 477, 4, 814-819, 2016.09, The mouse submandibular gland (SMG) is important organ for embryonic development, and branching morphogenesis is regulated by many molecules containing transcription factors. Real-time reverse transcriptase polymerase chain reaction revealed that the expression of Brachyury increased in the SMG and peaked between E12.5–E13.5, concomitant with the early stage of branching morphogenesis. The expression of Brachyury in SMG rudiments between E12.5–E13.5 was confirmed by western blotting. In addition, fibronectin and Btbd7 (regulated by fibronectin), which are both essential for cleft formation, were expressed strongly during the same period. The Sox2 and Wnt3a, which regulate cell growth, were also expressed strongly during E12.5–E13.5. On the other hand, cleft formation and branching morphogenesis was suppressed by knockdown of Brachyury gene, suggesting that Brachyury plays a central role in regulating cell growth and cleft formation in early-stage embryonic mouse salivary gland development..
7. Moriyama Masafumi, Miho Ohta, S Furukawa, Yurie Mikami, Akihito Tanaka, T Maehara, Hayashida Jun-Nosuke, M Yamauchi, Noriko Ishiguro, Hayashida Jun-Nosuke, Shintaro Kawano, Yukiko Ohyama, Tamotsu Kiyoshima, Seiji Nakamura, The diagnostic utility of labial salivary gland biopsy in IgG4-related disease.
, Mod Rheumatol., Epub ahead of print, 2016.03.
8. Mayumi Shimizu, Kazutoshi Okamura, Y Kise, Y Takeshita, Hiroko Furuhashi, W Weerawanich, Moriyama Masafumi, Yukiko Ohyama, Sachiko Furukawa, Seiji Nakamura, Kazunori Yoshiura, Effectiveness of imaging modalities for screening IgG4-related dacryoadenitis and sialadenitis (Mikulicz's disease) and for differentiating it from Sjögren's syndrome (SS), with an emphasis on sonography., Arthritis Res Ther., 10.1186/s13075-015-0751-x., 23, 17, 223, 2015.08.
9. T Maehara, Moriyama Masafumi, Shintaro Kawano, Hayashida Jun-Nosuke, S Furukawa, Miho Ohta, Akihito Tanaka, M Yamauchi, Yukiko Ohyama, Tamotsu Kiyoshima, Seiji Nakamura, Cytokine profiles contribute to understanding the pathogenic difference between good syndrome and oral lichen planus: two case reports and literature review. , Medicine, 1097/MD.0000000000000704., 94, 14,
, 2015.04.
10. S Furukawa, Moriyama Masafumi, Shintaro Kawano, Akihito Tanaka, T Maehara, Hayashida Jun-Nosuke, Yuichi Goto, Tamotsu Kiyoshima, Hideki Shiratsuchi, Yukiko Ohyama, Miho Ohta, Yumi Imabayashi, Seiji Nakamura, Clinical relevance of Küttner tumour and IgG4-related dacryoadenitis and sialoadenitis., Oral Diseases, 10.1111/odi.12259. , 21, 257-262, 257-262, 2014.06.
11. Takahiro Fujinaga, Kumamaru Wataru, Tsuyoshi Sugiura, Yosuke Kobayashi, Yukiko Ohyama, TATSUYA IKARI, Mitsuho Onimaru, Naonari Akimoto, Rumi Jogo, Yasuharu Takenoshita, Biological characterization and analysis of metastasis-related genes in cell lines derived from the primary lesion and lymph node metastasis of a squamous cell carcinoma arising in the mandibular gingiva. , Int J Oncol. , 10.3892/ijo.2014.2332., 44, 5, 1614-1624, 2014.03.
12. Goro Sugiyama, Yukiko Ohyama, Tamotsu Kiyoshima, Mayumi Shimizu, Kaneki Eisuke, Yasuharu Takenoshita, Metastatic adenocarcinoma of the mandibular condyle from uterine cervix: Report of a case., Oral Scinece Internatilnal, org/10.1016/S1348-8643(13)00028-1, 11, 40-44, 40-44, 2014.01.
13. Moriyama Masafumi, Akihito Tanaka, T Maehara, Yukiko Ohyama, Mayumi Shimizu, H Nakashima, Hayashida Jun-Nosuke, S Shinozaki, S Kubo, S Furukawa, Seiji Nakamura, Clinical characteristics of Mikulicz's disease as an IgG4-related disease., Clin Oral Investig. , 10.1007/s00784-012-0905-z., 17, 9, 1995-2002, 1995-2002, 2013.12.
14. Hayashida Jun-Nosuke, Seiji Nakamura, Toyoshima Takeshi, Moriyama Masafumi, Masanori Sasaki, Eiji Kawamura, Yukiko Ohyama, Kumamaru Wataru, Kamemitsu Shirasuna, Possible involvement of cytokines, chemokines and chemokine receptors in the initiation and progression of chronic GVHD., Bone Marrow Transplant, 10.1021/bi2018128, 48, 1, 115-123, 115-123, 2013.01.
15. Moriyama Masafumi, Hayashida Jun-Nosuke, Toyoshima Takeshi, Yukiko Ohyama, Shinozaki S, Tanaka Akihiko, Maehara T, Seiji Nakamura, Cytokine/chemokine profiles contribute to understanding the pathogenesis and diagnosis of primary Sjögren's syndrome., Clin Exp Immunol., 169, 1, 17-26, 17-26, 2012.06.
16. Goro Sugiyama, Hiroshi Takeuchi, Nagano Kouki, jing gao, Yukiko Ohyama, Yoshihide Mori, Masato Hirata, Regulated Interaction of Protein Phosphatase 1 and Protein Phosphatase 2A with Phospholipase C-Related but Catalytically Inactive Protein., Biochemistry, 51, 16, 3394-3403, 3394-3403, 2012.04.
17. Mayumi Shimizu, Moriyama Masafumi, Kazutoshi Okamura, Toshiyuki Kawazu, Toru Chikui, Tazuko K. Goto, Yukiko Ohyama, Seiji Nakamura, Kazunori Yoshiura, Sonographic diagnosis for Mikulicz disease., Oral Med Oral Pathol Oral Radiol Endod. , 108, 1, 105-113, 105-113, 2009.07.
18. Shimizu M, Okamura K, Yoshiura K, Ohyama Y, Nakamura S., Sonographic diagnosis of Sjogren's syndrome: evaluation of parotid gland vascularity as a diagnostic tool. , Oral Surg Oral Med Oral Pathol Oral Radiol Endod., 106(4) 567-594 2008, 2008.10.
19. Deshmukh US, Ohyama Y, Bagavant H, Guo X, Gaskin F, Fu SM., Inflammatory stimuli accelerate Sjögren's syndrome-like disease in (NZB x NZW)F(1) mice., Arthritis Rheum., 58(5):1318-1323 , 2008.04.
20. Shimizu M, Okamura K, Yoshiura K, Ohyama Y, Nakamura S, Kinukawa N, Sonographic diagnostic criteria for screening Sjogren's syndrome.., Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 102(1):85-93., 2006.07.
21. Toyoshima T, Nakamura S, Kumamaru W, Kawamura E, Ishibashi H, Ohyama Y, Sasaki M, Shirasuna K., Expression of tumor-associated antigen RCAS1 and its possible involvement in immune evasion in oral squamous cell carcinoma., J Oral Pathol Med., 2006.07.
22. Pal R, Deshmukh US, Ohyama Y, Fang Q, Kannapell CC, Gaskin F, Fu SM., Evidence for multiple shared antigenic determinants within Ro60 and other lupus-related ribonucleoprotein autoantigens in human autoimmune responses., J Immunol., 175(11):7669-77, 2005.12.
23. Shimizu M, Yoshiura K, Nakayama E, Kanda S, Nakamura S, Ohyama Y, Nakamura N., Multiple sialolithiasis in the parotid gland with Sjogren's syndrome and its sonographic findings--report of 3 cases., Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 10.1016/j.tripleo.2004.04.014, 99, 1, 85-92, 99(1):85-92, 2005.01.
24. Kumamaru W, Nakamura S, Kadena T, Yamada A, Kawamura E, Sasaki M, Ohyama Y, Toyoshima T, Hayashida J, Itoh K, Shirasuna K, T-cell Receptor Vb gene usage by T cells reactive with the tumor-rejection antigennnn SART-1 in oral squamous cell carcinoma, Int J Cancer, 108(5):686-695, 2004.02.
25. Kawamura E, Nakamura S, Sasaki M, Ohyama Y, Kadena T, Kumamaru W, Shirasuna K., Accumulation of oligoclonal T cells in the infiltrating lymphocytes in oral lichen planus., J Oral Pathol Med., 32, 5, 282-289, 32(5):282-9., 2003.05.
26. Tsunawaki S, Nakamura S, Ohyama Y, Sasaki M, Ikebe-Hiroki A, Hiraki A, Kadena T, Kawamura E, Kumamaru W, Shinohara M, Shirasuna K., Possible function of salivary gland epithelial cells as nonprofessional antigen-presenting cells in the development of Sjogren's syndrome., J Rheumatol., 29, 9, 1884-1896, 29(9):1884-96., 2002.09.
27. Sasaki M, Nakamura S, Ohyama Y, Shinohara M, Ezaki I, Hara H, Kadena T, Kishihara K, Yamamoto K, Nomoto K, Shirasuna K., Accumulation of common T cell clonotypes in the salivary glands of patients with human T lymphotropic virus type I-associated and idiopathic Sjogren's syndrome., J Immunol., 164, 5, 2823-2831, 164(5):2823-31, 2000.03.
28. Ohyama Y, Nakamura S, Hara H, Shinohara M, Sasaki M, Ikebe-Hiroki A, Mouri T, Tsunawaki S, Abe K, Shirasuna K, Nomoto K., Accumulation of human T lymphotropic virus type I-infected T cells in the salivary glands of patients with human T lymphotropic virus type I-associated Sjogren's syndrome., Arthritis Rheum., 10.1002/1529-0131(199811)41:11<1972::AID-ART12>3.0.CO;2-M, 41, 11, 1972-1978, 41(11):1972-8., 1998.11.
29. Nakamura S, Ikebe-Hiroki A, Shinohara M, Ohyama Y, Mouri T, Sasaki M, Shirasuna K, Nomoto K., An association between salivary gland disease and serological abnormalities in Sjogren's syndrome., J Oral Pathol Med., 10.1111/j.1600-0714.1997.tb00243.x, 26, 9, 426-430, 26(9):426-30., 1997.10.
30. Nakamura S, Ohyama Y, Shinohara M, Hiroki A, Nomoto K., Cytokine messenger RNA expression in the labial salivary glands of patients with Sjogren's syndrome (letter)., Arthritis Rheum., 40:989-90., 1997.01.
31. Aberrant HLA-DR antigen expression on acinr and ductal epithelial cells of labial salivary glands in patients with Sjogren's syndrome..
32. Mouri T, Nakamura S, Ohyama Y, Matsuzaki G, Shinohara M, Kishihara K, Hiroki A, Oka M, Shirasuna K, Nomoto K., T cell receptor V alpha and V beta gene usage by tumour-infiltrating lymphocytes in oral squamous cell carcinoma., Cancer Immunol Immunother., 10.1007/s002620050402, 45, 1, 61-61, 45(1):61., 1996.10.
33. Hiroki A, Nakamura S, Shinohara M, Gondo H, Ohyama Y, Hayashi S, Harada M, Niho Y, Oka M., A comparison of glandular involvement between chronic graft-versus-host disease and Sjogren's syndrome., Int J Oral Maxillofac Surg., 10.1016/S0901-5027(06)80062-7, 25, 4, 298-307, 25(4):298-307., 1996.08.
34. Matsuzaki G, Li XY, Ohyama Y, Nomoto K., Kinetics of serum granulocyte-colony stimulating factor (G-CSF) concentration and G-CSF receptor expression during G-CSF treatment of cyclophosphamide-treated mice., Int J Immunopharmacol., 10.1016/S0192-0561(96)00039-2, 18, 6-7, 363-369, 18(6-7):363-9., 1996.06.
35. Ohyama Y, Nakamura S, Matsuzaki G, Shinohara M,Ohyama Y, Oka M, Nomoto K., T-cell receptor V alpha and V beta gene use by infiltrating T cells in labial glands of patients with Sjogren's syndrome., Oral Surg Oral Med Oral Pathol Oral Radiol Endod., 10.1016/S1079-2104(05)80308-7, 79, 6, 730-737, 79(6):730-7., 1995.06.