Kyushu University Academic Staff Educational and Research Activities Database
List of Presentations
Koki Fujita Last modified date:2023.09.27

Assistant Professor / Sustainable Bioresources Science / Department of Agro-environmental Sciences / Faculty of Agriculture

1. The production of beta-thujaplicin in a suspension culture of Cupressus lusitanica.
2. mRNAs induced by elicitation in Cupressus lusitanica cultured cells..
3. Cloning of Cupressus lusitanica terpene synthese cDNA III.
4. Cytochrome P450 enzyme oxidizing terpinolene in Cupressus lusitanica cultured cell (II).
5. Factors at DHP preparation.
6. SPME analysis of the volatile terpenoids produced from Cupressus lusitanica cultured cell..
7. Pyrolysis of Lignin: Products Stemming from beta-O-4 subunits.
8. Cloning of Cupressus lusitanica terpene synthese cDNA IV, [URL].
9. Monoterpene emission from Cupressus lusitanica cell culture with fungal elicitor.
10. Induced isoprenoid biosynthesis in response to mechanical stress in cultured Cupressus lusitanica cells, [URL].
11. , [URL].
12. Stereo-selective oxidation of monoterpene in Cupressus lusitanica cells (2).
13. , [URL].
14. Pyrolysis of kenaf at lower temprature (3), [URL].
15. Stereo-selective oxidation of monoterpene in Cupressus lusitanica cells, [URL].
16. Antifungal activity of emitted monoterpenes from stressed Cupressus lusitanica cells, [URL].
17. Stereoselective oxidations of terpinolene in Cupressus lusitanica culture cells, [URL].
18. Pyrolysis of kenaf at lower temprature (IV).
19. Stereo-selective oxidation of monoterpene in Cupressus lusitanica cells (2) .
20. Stereo-selective oxidation of monoterpene in Cupressus lusitanica cells (3) .
21. Signaling effect of monoterpenes emitted from Cupressus lusitanica.
22. Pyrolysis of kenaf at lower temprature (V).
23. Metabolism of terpinolene which is produced by fungal attack in Cupressus lusitanica culture cells, [URL].
24. The signaling effect of volatile monoterpenes emitted from elicited Cupressus lusitanica cultured cells, [URL].
25. Longifolene as a precursor of plant self‐defensive compounds, [URL].
26. Terpinoene metabolism in Cupressus lusitanica cultured cell .
27. Facilitation of phytoalexine production by gaseous monoterpene in Cupressus lusitanica cell culture, [URL].
28. Oxidative metabolism of monoterpenes in Cupressus lusitanica., [URL].
29. Anti-ant activity of Humulene derivatives, [URL].
30. Bioactivities of epoxide and episulfide derived from medium ring sesquiterpene hydrocarbons, [URL].
31. Oxidative metabolism of monoterpene in Cupressus lusitanica culture cells, [URL].
32. Lignin formation on Cupressus lusitanica cultured cell at phytoalexine producing condition..
33. Monoterpene biosynthesis -General idea and a case of terpinolene metabolism in Cupressus lusitanica- .
34. , [URL].
35. , [URL].
36. The relationship between the time courses of the β-thujaplicin production and of the activities of terpinolene oxygenases in Cupressus lusitanica cultured cells, [URL].
37. Terpinolene is a intermediate of beta-thujhaplicin biosynthesis.
38. Signal transduction with olefin monoterpene in Cuplessus lusitanica cultured cell.
39. Koki Fujita, Yasufumi Bunyu, Ken-ichi Kuroda, Tatsuya Ashitani, Yuji Tsutsumi, Terpinolene is the first olefin monoterpene intermediate to a tropolone, β-thujaplicin –Potential novel pathway to tropolone ring–, TERPNET2013, 2013.06, [URL], β-Thujaplicin (Hinokitiol) is a wood monoterpene and a tropolonecompound, which has unique conjugated seven-membered ring. Because of its strong antifungal and antitumor activities, β-thujaplicin has been used in several fields. However, the biosynthetic pathway of it has not been elucidated. Our group had proved that geranyl pyrophosphate (GPP) was the starting material of this pathway using Cupressus lusitanica cell cultures with radioisotope feeding experiment. In addition, our previous study suggested that terpinolene was the next metabolite from GPP based on the results of terpene synthase assay. In this study, we performed feeding experiment of deuterium-labeled terpinolene into the cultured cell of C. lusitanica in order to determine whether terpinolene is intermediate of β-thujaplicin biosynthesis. GC/MS analysis of cell extract from the culture labeled-terpinolene fed revealed the existence of the peak of labeled β-thujaplicin which was not observed in treatment of non-labeled terpinolene. Identification of labeled β-
thujaplicin was confirmed by mass spectrum assignment. This indicated that terpinolene was the first olefin monoterpene intermediate of β-thujaplicin biosynthesis. There has been no report that tropolone compounds are biosynthesized via terpene biosynthesis system, so the result suggests the existence of a novel biosynthetic pathway to produce conjugated seven-membered ring. .
40. Koki Fujita, Ryo Kanbe, Tatsuya Yagi, Ransika De Alwis, Tatsuya Ashitani, Yuji Tsutsumi, Air born defense signal transduction cascade with monoterpenes on Cupressus lusitanica culture cells , TERPNET2013, 2013.06, [URL], Many experiments for plant-plant communication had been conducted widely in field or laboratory; experiments used whole plant or organs like leaf, root and shoot. However, a model experiment system, which is simple and free from the influence of environment, is favorable. Our group has used cultured cell of Cupressus lusitanica to investigate the chemical communication as a model. This cell line produced 10 hydrocarbon monoterpenes as well as β-thujaplicin, which is strong phytoalexin, when they were elicited. Because these monoterpenes were emitted into atmosphere, special roles as vapor were expected previously. In this work, the cells were exposed with artificial vapor monoterpenes and then headspace gases of the culture flasks were analyzed with SPME and GCMS. As results, when culture cells were exposed with artificial sabinene, de novo produced γ-terpinene and p-cymene were detected in headspace gas. When culture cells were exposed with artificial γ-terpinene as well, the products were p-cymene and terpinolene. In case of the p-cymene exposure, terpinolene was emitted into the air. However, this phenomenon was not observed with the exposure of other monoterpenes, such as α-pinene, β-pinene, limonene, myrcene and terpinolene. These results strongly suggest “signal transfer cascade” which starts from sabinene and followed by γ-terpinene, p-cymene and, then, end at terpinolene. Though terpinolene didn’t cause air born signaling, it facilitated the starting time of β-thujaplicin production. That is, by pathogen or insect attack, C. lusitanica cells implement a signal cascade to prepare β-thujaplicin production of innate plant or adjacent leaves. The results led us to propose dual functions of the cascade as shown in Figure. 1. The signal can spread over longer range with the cascade of monoterpenes. 2. Tree can measure the distance from pathogen-invaded tree by depending on the kinds of monoterpenes recognized..
41. Time course change of olefin monoterpenes involved in sigmnal transfar on Cupressus lusitanica cultured cell..
42. Signalling effects of the volatile monoterpene olefins from Cupressus lusitanica cultured cells. .
43. We found very quick de novo production of terpenes by terpensynthases and its time course information revealed signaling mechanism., [URL].
44. Suguru wada, Koki Fujita, Yuji Tsutsumi, Analysis of lignin structural changes within a growth ring of Populus alba xylem using Laser micro dissection and Py-GC-MS, International Symposium on Wood Science and Technology 2015, 2015.03, Lignin is the phenylpropanoid polymer composed of three monolignols through the several linkages generated by the radical couplings. The biosynthesis of monolignols has ever been studied using mainly homogenized organs or tissues which contains several types of cells. However, many reports indicated that deposition of lignin is heterogeneous, and lignin biosynthesis may be highly regulated depending on cell types or tissues. Further, it was reported that some genes related in monolignol biosynthesis were expressed in ray parenchyma cells in the lignified area1), and CWPO-C that is capable of lignin polymerization located in the cell corners of the matured xylem2). These reports suggest that lignification still keeps proceeding in the fiber cells after their programmed cell death. Recently, in cell culture systems, lignification of tracheary element cell walls has been indicated to continue to increase after the cell death3). In order to understand more detailed lignification process, we have tried to obtain the cell-type- or tissues- specific information on lignin biosynthesis mechanism.
In this study we employed the laser micro dissection (LMD) technique to dissect a current year growth ring of the Populus alba secondary xylem that contains approximately 70 cells in a line (Fig.) into several sections, at least 8 stage of different lignified levels. The dissected sections were subjected to Py-GC-MS, and the pyrolysis products and S/G ratio derived from β-O-4 linked lignin in the dissected samples were determined. The increase of pyrolysis products and decrease of S/G ratio along with the radial direction from cambial to pith within a growth ring suggested that lignification continues in the mature xylem fiber cells in which lignification has generally been thought to be completed. On the other hand acetocarmine staining of nuclei and fragments of DNA was observed only in the cells within the area of 20th or 30th from cambium (Fig.), but not in the older cells than them, suggesting that the fiber cells in the older cells area than 20th or 30th cells were already suffered from programmed cell death. These results imply that lignification in the fiber cells still continues even after programmed cell death, probably with the assistance of ray parenchyma cells..
45. Development of bamboo biorefinery
Production of phenolic monomers from bamboo lignin.
46. Characterizations of polyurethane foam prepared from liquefied bamboo and liquefaction of bamboo by PEG-bisulfite method.
47. Metabolic Engineering of Basidiomycete for the Utilization of Hemicellulose of Bamboo.
48. Liquefaction of bamboo by PEG-bisulfite method.
49. Liquefaction of bamboo by PEG-bisulfite method.
50. Carbon material from liquefied bamboo-lignin by PEG-bisulfite method.
51. Preparation of carbon fiber by electrospinning using liquefied bamboo from PEG-bisulfite method.
52. Potential utilization of bamboo alkali lignin as an antioxidant and α-glucosidase inhibitory activator.
53. Development of syringyl lignin-cellulose conjugate film using ferulic acid scaffold mediated by rCWPO-C.
54. Plant growth regulation by CWPO-C through the IAA metabolism.
55. The chemical response of Cupressus lusitanica seedling against chemical and physical stimulations.
56. Attempt to establish analysis of some sesquiterpenes contained in cedar using GC-MS for JAS standards.
57. Effect on the plant growth and its regulatory manner by the knockout of CWPO-C orthologous, AtPrx2, AtPrx25, AtPrx71 in Arabidopsis thaliana.
58. Polymerization and aggregation morphology of syringyl monolignols during the formation of artificial cell wall using rCWPO-C.
59. The cationic cell-wall-bound peroxidase (CWPO-C) involved in plant growth and lignification in Poplar.
60. Effect of different amounts of cedar lumber on psychological and physiological responses.