Kyushu University Academic Staff Educational and Research Activities Database
List of Reports
Ken-ichi Honjoh Last modified date:2024.04.11

Associate Professor / Division of Food Science and Biotechnology / Department of Bioscience and Biotechnology / Faculty of Agriculture


Reports
1. Injured Microbes(4)Injury of Listeria monocytogenes in Vegetable Field.
2. Effects of Sucrose Monopalmitate under Microbubble Generation and/or Slightly Acidic Hypochlorous Water on Salmonella Enteritidis Adsorbed on the Surface of Various Materials
Effects of sucrose monopalmitate under microbubble generation and/or slightly acidic hypochlorous water(SAHW)on Salmonella Enteritidis were investigated in suspension and on the surface of various materials. In suspension, S. Enteritidis was not injured by the treatment with sucrose palmitate at 100 mg/L under generation of microbubble, though the cells decreased the viable counts by 3.0 log by the treatment with SAHW at 30 mg/L. In contrast, the viable counts decreased only by 1.6 log when S. Enteritidis was treated with sucrose palmitate under microbubble generation and subsequent treatment with SAHW. The viable counts of S. Enteritidis adsorbed on the surface of cabbage leaf decreased by 0 . 3 , 1 . 3 , and 1 . 3 log by the treatment with sucrose monopalmitate under microbubble generation, SAHW and sucrose monopalmitate under microbubble generation followed by SAHW. By the same treatments, the viable counts of S. Enteritidis decreased by 0.7, 3.2, and 4.2 log in cells adsorbed on nitrocellulose membrane, 0.6, 1.2, and 2.3 log in cells on PVDF membrane, 0.7, 1.0, and 0.9 log in cells on Hybond-N+membrane, respectively. It seems that the bacteria adsorbed on the surface of PVDF membrane provide a model system to investigate the efficacy of detergents and sterilizers on decontamination of vegetables..
3. Genotyping of Campylobacter jejuni Isolated from Chicken Meat and Guts Using Random Amplified Polymorphic DNA (RAPD) Typing and Automated Ribotyping.
4. Contamination and survival of Salmonella on leaf vegetable during cultivation
Salmonella contamination of spinach leaf during cultivation was investigated. Salmonella Enteritidis expressing green fluorescent protein(S . Enteritidis-GFP)was used in this study. S . Enteritidis-GFP was detected from soil, which had been initially inoculated with the bacterium at more than 2 logCFU/g after 35-d cultivation. The viable S . Enteritidis-GFP counts in soil ranged from 4 to 7 logCFU/g in proportion to the initial inoculum from 2 to 8 logCFU/g after 35-d cultivation. All the 6 spinach plants were negative for S . Enteritidis-GFP by the qualitative test, when spinach had been cultivated in a soil initially contaminated with the bacterium at less than 3.8 logCFU/g. In contrast, all the 6 plants were positive for the bacterium when cultivated in a soil contaminated with more than 6.0 logCFU/g. However, the leaves of all 9 plants were negative for S . Enteritidis-GFP after surface sterilization, suggesting the surface contamination. At 7d after overhead irrigation of water contaminated with S . Enteritidis- GFP at less than 3.1 logCFU/mL, all the spinach plants were negative for the bacterium in 18 separately cultivated spinach plants..
5. Combined Effects of Surfactants and Preservatives on the Antibacterial Activity of Green Tea Extracts.
6. The effect of Lunularic acid on the freezing tolerance of Chlorella vulgaris C-27.
7. Rapid Detection Method of Salmonella-specific PCR Product by DNA-immobilized Quartz Crystal Microbalance.
8. HONJOH Ken-ichi, NAGAISHI Noriko, MATSUMOTO Hiroko, HATANO Shoji, EXPRESSION OF CHLORELLA hiC6(lea) GENE IN TRANSGENIC TOBACCO PLANTS, Plant and cell physiology, Vol.38, p.s95, 1997.03.