Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
MASAO SATO Last modified date:2023.06.26

Professor / Food Science & Technology / Department of Bioscience and Biotechnology / Faculty of Agriculture

1. Xingyu Yuan, Rika Nagamine, Yasutake Tanaka, Wei-Ting Tsai, Zhe Jiang, Ai Takeyama, Katsumi Imaizumi, Masao Sato, The effects of dietary linoleic acid on reducing serum cholesterol and atherosclerosis development are nullified by a high-cholesterol diet in male and female apolipoprotein E-deficient mice, British Journal of Nutrition, 10.1017/S0007114522001325, 2023.03, Linoleic acid (LA) has a two-sided effect with regards to serum cholesterol lowering and pro-inflammation, although whether this fatty acid reduces serum cholesterol and the development of atherosclerosis under high-cholesterol conditions has yet to be ascertained. In this study, we examine the effects of dietary LA on reducing serum cholesterol and atherosclerosis development under high-cholesterol conditions. Male and female apolipoprotein E-deficient (Apo E-/-) mice were fed AIN-76-based diets containing 10% saturated fatty acids and 0.04 % cholesterol (SFA), 10% LA and 0.04% low cholesterol (LALC), or 10% LA and 0.1% high cholesterol (LAHC) for 9 weeks. The results revealed significant reduction in serum cholesterol levels and aortic lesions with increasing levels of pro-inflammatory biomarkers (urinary isoprostane and aortic MCP-1 mRNA) in male and female LALC groups compared with those in the SFA groups (P < 0.05). Furthermore, whereas there were significant increases in the serum cholesterol levels and aortic lesions (P < 0.05), there was no difference in aortic MCP-1 mRNA levels in male and female LAHC groups compared with those in the LALC groups. A high dietary intake of cholesterol eliminated the serum cholesterol-lowering activity of LA but had no significant effect on aortic inflammation in either male or female ApoE-/- mice. The inhibitory effect of LA on arteriosclerosis is cancelled by a high-cholesterol diet due to a direct increase in serum cholesterol levels. Accordingly, serum cholesterol levels might represent a more prominent pathogenic factor than aortic inflammation in promoting the development of atherosclerosis..
2. Iwasaki, Wakana, Yoshida, Ryo,Liu, Hongxia, Hori, Shota, Otsubo, Yuki, Tanaka, Yasutake, Sato, Masao,Ishizuka, Satoshi , The ratio of 12α to non-12-hydroxylated bile acids reflects hepatic triacylglycerol accumulation in high-fat diet-fed C57BL/6J mice, Scientific Reports, 10.1038/s41598-022-20838-9, Article number 16707, 2022.12.
3. Hashiguchi, Kiwa, Kikuchi, Takuma, Mizokami, Takuya, Sato, Masao, Nishimukai, Megumi, The levels of plasma plasmalogen in retired female rats decrease by ovariectomy and intake of cholesterol-diet, Bioscience, Biotechnology and Biochemistry, 10.1093/bbb/zbac148, 86, 11, 1543-1551, 2022.11.
4. Xingyu Yuan, Yutaro Minobe, Yasutake Tanaka, Yumi Fukuda, Yumiko Furukawa, Motonori Miyago, Takuya Mizokami, Wei-Ting Tsai, Zhe Jiang, Li-Tao Tong, Taiki Akasaka, Bungo Shirouchi, Yoshiko Toyosawa, Toshihiro Kumamaru, Masao Sato, α-globulin-rich rice cultivar, low glutelin content-1 (LGC-1), decreases serum cholesterol concentration in exogenously hypercholesterolemic rat, Journal of the Science of Food and Agriculture, 101, 15, 6417-6423, 2021.12.
5. Ai Takeyama, Asami Teramoto, Tianyu Wang, Takuya Hayashi, Yasutake Tanaka, Masao Sato, Bungo Shirouchi, Dietary lysophospholipids reduce lymphatic cholesterol transport compared with dietary phospholipids in thoracic lymph-duct cannulated rats, Lipids, 10.1002/lipd.12319, 56, 579-590, 2021.05.
6. Lee, J.-Y., Shimizu, H., Hagio, M., Fukiya, S., Watanabe, M., Tanaka, Y., Joe, G.-H., Iwaya, H., Yoshitsugu, R., Kikuchi, K., Tsuji, M., Baba, N., Nose, T., Tada, K., Hanai, T., Hori, S., Takeuchi, A., Furukawa, Y., Shirouchi, B., Sato, M., Ooka, T., Ogura, Y., Hayashi, T., Yokota, A., Ishizuka, S, 12α-Hydroxylated bile acid induces hepatic steatosis with dysbiosis in rats, Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 10.1016/j.bbalip.2020.158811, 2020.12, There is an increasing need to explore the mechanism of the progression of non-alcoholic fatty liver disease. Steroid metabolism is closely linked to hepatic steatosis and steroids are excreted as bile acids (BAs). Here, we demonstrated that feeding WKAH/HkmSlc inbred rats a diet supplemented with cholic acid (CA) at 0.5 g/kg for 13 weeks induced simple steatosis without obesity. Liver triglyceride and cholesterol levels were increased accompanied by mild elevation of aminotransferase activities. There were no signs of inflammation, insulin resistance, oxidative stress, or fibrosis. CA supplementation increased levels of CA and taurocholic acid (TCA) in enterohepatic circulation and deoxycholic acid (DCA) levels in cecum with an increased ratio of 12α-hydroxylated BAs to non-12α-hydroxylated BAs. Analyses of hepatic gene expression revealed no apparent feedback control of BA and cholesterol biosynthesis. CA feeding induced dysbiosis in cecal microbiota with enrichment of DCA producers, which underlines the increased cecal DCA levels. The mechanism of steatosis was increased expression of Srebp1 (positive regulator of liver lipogenesis) through activation of the liver X receptor by increased oxysterols in the CA-fed rats, especially 4β-hydroxycholesterol (4βOH) formed by upregulated expression of hepatic Cyp3a2, responsible for 4βOH formation. Multiple regression analyses identified portal TCA and cecal DCA as positive predictors for liver 4βOH levels. The possible mechanisms linking these predictors and upregulated expression of Cyp3a2 are discussed. Overall, our observations highlight the role of 12α-hydroxylated BAs in triggering liver lipogenesis and allow us to explore the mechanisms of hepatic steatosis onset, focusing on cholesterol and BA metabolism. .
7. Zhe Jiang, Takuya Hayashi, Kentaro Kashima, Kayo Kurotani, Bungo Shirouchi, Tetsuya Mizoue, Masao Sato, Alteration of Serum Phospholipid n-6 Polyunsaturated Fatty Acid Compositions in Nonalcoholic Fatty Liver Disease in the Japanese Population
A Cross-Sectional Study, Lipids, 10.1002/lipd.12251, 2020.06, We performed a cross-sectional study on 215 Japanese employees aged 20–68 years to investigate the association between NAFLD and serum phospholipid fatty acid composition. NAFLD was diagnosed by ultrasonography. The fatty acid composition between the control and NAFLD groups was compared, and the inverse probability of treatment weighting (IPTW) was performed to eliminate each confounding effect of sex, smoking status, BMI, insulin resistance, dietary cholesterol, and salt intake. A receiver operating characteristic (ROC) curve analysis was performed to evaluate the NAFLD prediction accuracy of fatty acids. Seventy-one subjects were diagnosed with NAFLD. Their serum phospholipid dihomo-γ-linolenic acid (DGLA) level was significantly higher after adjusting for each variable using IPTW. In the ROC analysis, the ratio of ARA to DGLA had an area under the curve (AUC) of 0.763. By combining the ratio of ARA to DGLA with the ratio of AST to ALT, AUC increased to 0.871. In conclusion, NAFLD subjects in a Japanese working population have higher serum phospholipid DGLA. Results of the IPTW and ROC analysis indicated that serum PL DGLA and the ratio of ARA to DGLA provide diagnosis information on the fatty liver that is different to AST and ALT and improve the accuracy of fatty liver prediction, owning potential value as serum biomarkers..
8. Y. Tanaka, M. Ono , M. Miyago, T. Suzuki, Y. Miyazaki, M. Kawano, M. Asahina, B. Shirouchi, K. Imaizumi, M. Sato , Low utilization of glucose in the liver causes diet-induced hypercholesterolemia in exogenously hypercholesterolemic rats., PLOS ONE, 10.1371/journal.pone.0229669.g003, 2020.03, [URL], Exogenously hypercholesterolemic (ExHC) rats develop diet-induced hypercholesterolemia (DIHC) when fed with dietary cholesterol. Previously, we reported that, under the high-sucrose-diet-feeding condition, a loss-of-function mutation in Smek2 results in low activity of fatty acid synthase (FAS) followed by the shortage of hepatic triacylglycerol content in ExHC rats and the onset of DIHC. However, the relationship between the Smek2 mutation and FAS dysfunction is still unclear. Here, we focused on carbohydrate metabolism, which provides substrates for FAS, and analyzed carbohydrate and lipid metabolisms in ExHC rats to clarify how the deficit of Smek2 causes DIHC. Male ExHC and SD rats were fed high-sucrose or high-starch diets containing 1% cholesterol for 2 weeks. Serum cholesterol levels of the ExHC rats were higher, regardless of the dietary carbohydrate. Hepatic triacylglycerol levels were higher in only the SD rats fed the high-sucrose diet. Moreover, the ExHC rats exhibited a diabetes-like status and accumulation of hepatic glycogen and low hepatic mRNA levels of liver-type phosphofructokinase (Pfkl), which encodes a rate-limiting enzyme for glycolysis. These results suggest that the glucose utilization, particularly glycolysis, is impaired in the liver of ExHC rats. To evaluate how the diet with extremely low glucose affect to DIHC, ExHC.BN-Dihc2BN, a congenic strain that does not develop DIHC, and ExHC rats were fed a high-fructose diet containing 1% cholesterol for 2 weeks. The serum cholesterol and hepatic triacylglycerol levels were similar in the strains. Results of water-soluble metabolite analysis with primary hepatocytes, an increase in fructose-6-phosphate and decreases in succinate, malate and aspartate in ExHC rats, support impaired glycolysis in the ExHC rats. Thus, the Smek2 mutation causes abnormal hepatic glucose utilization via downregulation of Pfkl expression. This abnormal glucose metabolism disrupts hepatic fatty acid synthesis and causes DIHC in the ExHC rats..
9. Kanasaki, A., Iida, T., Murao, K., Shirouchi, B., Sato, M., D-Allulose enhances uptake of HDL-cholesterol into rat’s primary hepatocyte via SR-B1, Cytotechnology, 10.1007/s10616-020-00378-8, 2020.02.
10. Shirouchi, B., Yanagi, S., Okawa, C., Koga, M., Sato, M., 6-Ketocholestanol suppresses lipid accumulation by decreasing FASN gene expression through SREBP-dependent regulation in HepG2 cells, Cytotechnology, org/10.1007/s10616-019-00368-5, 72, 175-178, 2020.01.
11. Satoshi Wanezaki, Shintaro Saito, Nao Inoue, Nobuhiko Tachibana, Bungo Shirouchi, Masao Sato, Teruyoshi Yanagita, Koji Nagao, Soy β-conglycinin peptide attenuates obesity and lipid abnormalities in obese model oletf rats, Journal of oleo science, 10.5650/jos.ess20010, 69, 5, 495-502, 2020.01, We previously reported that soy β-conglycinin (βCG) improves obesity-induced metabolic abnormalities, but not obesity, in obese model Otsuka Long-Evans Tokushima fatty (OLETF) rats. In the present study, we aimed to investigate the effects of βCG-derived peptide consumption on obesity and lipid abnormality in OLETF rats. To this end, wild-type Long-Evans Tokushima Otsuka and OLETF rats were provided a normal diet containing 20% casein for four weeks as a control. In addition, we prepared βCG peptide by enzymatic hydrolysis, and OLETF rats were fed a diet in which half of the casein was replaced by βCG peptide (βCG peptide group). Consequently, rats in the βCG peptide group showed decreased abdominal white adipose tissue weight and lipid content (serum and liver triglycerides, and serum and liver cholesterol) compared to control OLETF rats. Further analysis demonstrated that βCG peptide consumption decreased lipogenic enzyme activity and increased lipolytic enzyme activity in the liver of OLETF rats. In addition, suppressive effects on both synthesis and absorption of cholesterol were observed in βCG peptide-fed OLETF rats. These findings suggest that peptidization of βCG enhanced the anti-obese and hypolipidemic effects of βCG..
12. Miki Fujiwara, Daichi Kuwahara, Masahiro Hayashi, Takeshi Zendo, Masao Sato, Jiro Nakayama, Kenji Sonomoto, Lowering effect of viable Pediococcus pentosaceus QU 19 on the rise in postprandial glucose, Bioscience of Microbiota, Food and Health, 10.12938/BMFH.19-041, 39, 2, 57-64, 2020.01, In the present study, we investigated the glucose-decreasing action of lactic acid bacteria (LAB). The finding of this study could be helpful for people in controlling their blood sugar levels. The LAB candidate was isolated from a Japanese fermented food and identified as Pediococcus pentosaceus by an analysis of its genome sequence. Postprandial blood glucose elevation was investigated using oral starch tolerance tests in mice. Normal mice were fed starch and lyophilized cells of P. pentosaceus QU 19 at the same time. Even without pre-administration of P. pentosaceus QU 19, elevation of the blood glucose level was significantly suppressed by the intake of P. pentosaceus QU 19 at the same time as oral administration of starch. According to the results for its survival in simulated digestive juice and the reduction of blood glucose level in mice, P. pentosaceus QU 19 has potential hypoglycemic activity. In vitro measurements revealed that the glucose-decreasing action of P. pentosaceus QU 19 is probably caused by the glucose assimilation of the strain, not the inhibition of carbohydrate-splitting enzymes which has been reported for other LABs previously. These findings indicate that specific strains of LAB, especially P. pentosaceus QU 19, and foods fermented by LAB may be beneficial for people who must manage glucose ingestion..
13. Kanasaki, A., Jiang, Z., Mizokami, T., Shirouchi, B., Iida, T.a, Nagata, Y., Sato, M., Dietary D-allulose alters cholesterol metabolism in Golden Syrian hamsters partly by reducing serum PCSK9 levels, Journal of Functional Foods, 10.1016/j.jff.2019.103429, 60, 103429, 2019.09.
14. Niibo, M., Shirouchi, B., Umegatani, M., Morita, Y., Ogawa, A., Sakai, F., Kadooka, Y., Sato, M, Probiotic Lactobacillus gasseri SBT2055 improves insulin secretion in a diabetic rat model, Journal of Dairy Science, 10.3168/jds.2018-15203, 102, 2, 997-1006, 2019.02.
15. Inhibition of Niemann-Pick C1-Like 1 by Ezetimibe Reduces Dietary 5β,6β-Epoxycholesterol Absorption in Rats
© 2019, Springer Science+Business Media, LLC, part of Springer Nature. Purpose: Oxycholesterols (OCs) are produced from cholesterol by oxidation of the steroidal backbone and side-chain. OCs are present in blood and evidence suggests their involvement in disease development and progression. However, limited information is available regarding the absorption mechanisms and relative absorption rates of dietary OCs. Although ezetimibe is known to inhibit intestinal cholesterol absorption via Niemann-Pick C1-Like 1 (NPC1L1), whether it also inhibits dietary OC absorption is unclear. Methods: We investigated the effects of ezetimibe on OC absorption in rats fed an OC-rich diet containing 10 different OCs. We collected lymphatic fluid using permanent cannulation of the thoracic duct and quantified OC levels. Results: Ezetimibe treatment significantly reduced the apparent absorption of 5β,6β-epoxycholesterol (5,6β-epoxy) and its levels in the proximal intestinal mucosa in OC-fed rats. Using in silico analyses, the binding energy of NPC1L1 N-terminal domain (NPC1L1-NTD) and 5,6β-epoxy was found to be similar to that of NPC1L1-NTD and cholesterol, suggesting that polar uncharged amino acids located in the steroidal part of 5,6β-epoxy were involved. Conclusion: Our results indicate that ezetimibe-mediated inhibition of dietary OC absorption varies depending on the specific OC, and only the absorption of 5,6β-epoxy is significantly reduced..
16. Takeyama, A., Nagata, Y., Shirouchi, B., Nonaka, C., Aoki, H., Haraguchi, T., Sato, M., Tamaya, K., Yamamoto, H., Tanaka, K., Dietary Sparassis crispa reduces body fat mass and hepatic lipid levels by enhancing energy expenditure and suppressing lipogenesis in rats, Journal of Oleo Science, 10.5650/jos.ess18043, 67, 9, 1137-1147, 2018.09, Accumulation of abdominal fat triggers metabolic syndrome, which is a cluster of metabolic abnormalities, such as dyslipidemia, glucose intolerance, insulin resistance or hyperinsulinemia, and hypertension, that leads to the development of diabetes and cardiovascular disease. Mushrooms have been used as a foodstuff and folk medicine worldwide. Among these mushrooms, Sparassis crispa (SC) is a relatively newly cultivated edible and medicinal mushroom, which has been reported to have anti-diabetic and anti-hypertensive properties. However, little is known about the anti-obesity and anti-hyperlipidemic properties of SC. In the present study, we investigated the effects of dietary SC on lipid metabolism and energy expenditure in Sprague-Dawley rats with diet-induced obesity and diabetes, and conducted respiratory gas analysis to determine how energy metabolism is altered by SC. After feeding periods of 3 and 7 weeks, dietary SC had significantly reduced hepatic triacylglycerol and cholesterol contents in a dose-dependent manner. These changes were attributable to suppression of fatty acid and cholesterol synthesis in the liver and increased insulin sensitivity in the body. In addition, after a feeding period of 6 weeks, dietary SC significantly increased energy expenditure through carbohydrate oxidation, reducing abdominal fat mass after 7 weeks. In conclusion, our results indicate that in addition to the previously reported anti-diabetic and anti-hypertensive activities, dietary SC exhibits anti-obesity and anti-hyperlipidemic activities that help protect against metabolic syndrome..
17. Shirouchi, B., Yamanaka, R., Tanaka, S., Kawatou, F., Hayashi, T., Takeyama, A., Nakao, A., Goromaru, R., Iwamoto, M., Sato, M., Quantities of phospholipid molecular classes in japanese meals and prediction of their sources by multiple regression analysis, Journal of Nutritional Science and Vitaminology, 10.3177/jnsv.64.215, 64, 3, 215-221, 2018.05, Dietary intake of total phospholipids (PLs) accounts for approximately 10% of total dietary lipids. Each PL molecular class has various beneficial effects on health. However, limited information is available regarding the intake of phosphatidylcholine (PC), phos-phatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), lysophos-phatidylcholine (LPC) and sphingomyelin (SM) among Japanese people, and the relevant food sources. In this study, we quantified the contents of PC, PE, PI, PS, LPC, and SM in 120 meal samples served in a Japanese company’s dormitory and cafeteria. Additionally, we measured the weight of each food group and estimated the contents of nutrients in these meals. Furthermore, we conducted a stepwise multiple regression analysis to identify pre-dictors (food groups) of each PL class intake. The contents of total PL, PC, PE, PI+PS, LPC, and SM (mean value) were 4.44, 2.17, 0.632, 0.123, 0.313, and 0.127 g/d, respectively. These values were considered as daily PL intake in accordance with data (three macronu-trients, vitamins, and minerals) from our study and the National Health and Nutrition Survey (NHNS) Japan, 2015. The content of eggs, meat, fish and shellfish, milk, pulses, fruits, mushrooms, cereals, and fats and oils in the meals predicted the PL and PC contents. The content of eggs, pulses, and mushrooms in the meals predicted the PE contents. Our results determined the daily intake of PL molecular classes among Japanese people and the food sources of PC and PE, and suggested that multiple regression analysis is useful for the prediction of food sources of bioactive components.
18. Kimura Y, Yasuda K, Kurotani K, Akter S, Kashino I, Hayabuchi H, Sato M, Mizoue T, Circulating ferritin concentrations are differentially associated with serum adipokine concentrations in Japanese men and premenopausal women., Eur J Nutr,, 10.1007/s00394-016-1285-y, 56, 8, 2497-2505, 2017.12, PURPOSE:
Increased iron storage, as measured by circulating ferritin, has been linked to an increased risk of various diseases including diabetes. We examined the association of circulating ferritin with serum adiponectin, leptin, resistin, plasminogen activator inhibitor-1 (PAI-1), and visfatin levels.
We conducted a cross-sectional study among 429 Japanese employees (284 men and 145 premenopausal women, mean age: 42.5 ± 10.5 years). Serum adipokines were measured using Luminex suspension bead-based multiplexed array, and serum ferritin was determined using a chemiluminescence immunoassay. Multivariable regression analysis was performed to calculate mean concentrations of adipokine according to the tertile of ferritin concentrations with adjustment for potential confounders.
Leptin and visfatin concentrations increased with increasing ferritin concentrations in men after multivariable adjustment of physical activity, smoking, alcohol use, and body mass index (P for trend = 0.02 and 0.01 for leptin and visfatin, respectively). Serum ferritin concentrations were inversely and significantly associated with adiponectin in women (P for trend = 0.01). Resistin and PAI-1 were not appreciably associated with ferritin concentration.
Increased iron storage may be associated with higher circulating concentrations of leptin and visfatin in men and with lower concentrations of adiponectin in women..
19. Matsuoka R, Shirouchi B, Umegatani M, Fukuda M, Muto A, Masuda Y, Kunou M, Sato M, Dietary egg-white protein increases body protein mass and reduces body fat mass through an acceleration of hepatic β-oxidation in rats., Br J Nutr., 10.1017/S0007114517002306, 118, 6, 423-430, 2017.09, Egg-white protein (EWP) is known to reduce lymphatic TAG transport in rats. In this study, we investigated the effects of dietary EWP on body fat mass. Male rats, 4 weeks old, were fed diets containing either 20 % EWP or casein for 28 d. Carcass protein levels and gastrocnemius leg muscle weights in the EWP group were significantly higher than those in the casein group. In addition, carcass TAG levels and abdominal fat weights in the EWP group were significantly lower than those in the casein group; adipocyte size in abdominal fat in the EWP group was smaller than that in the casein group. To identify the involvement of dietary fat levels in the rats, one of two fat levels (5 or 10 %) was added to their diet along with the different protein sources (EWP and casein). Abdominal fat weight and serum and hepatic TAG levels were significantly lower in the EWP group than in the casein group. Moreover, significantly higher values of enzymatic activity related to β-oxidation in the liver were observed in the EWP group compared with the casein group. Finally, abdominal fat weight reduction in the EWP group with the 10 % fat diet was lower than that in the EWP group with the 5 % fat diet. In conclusion, our results indicate that, in addition to the inhibition of dietary TAG absorption reported previously, dietary EWP reduces body fat mass in rats through an increase of body protein mass and the acceleration of β-oxidation in the liver..
20. Eri Hiraki, Shoko Furuta, Rika Kuwahara, Naomichi Takemoto, Toshiro Nagata, Taiki Akasaka, Bungo Shirouchi, Masao Sato, Koichiro Ohnuki, Kuniyoshi Shimizu, Anti-obesity activity of Yamabushitake (Hericium erinaceus) powder in ovariectomized mice, and its potentially active compounds, Journal of Natural Medicines, 10.1007/s11418-017-1075-8, 71, 3, 482-491, 2017.07, © 2017, The Japanese Society of Pharmacognosy and Springer Japan. Hericium erinaceus (H. erinaceus) improves the symptoms of menopause. In this study, using ovariectomized mice as a model of menopause, we investigated the anti-obesity effect of this mushroom in menopause. Mice fed diets containing H. erinaceus powder showed significant decreases in the amounts of fat tissue, plasma levels of total cholesterol, and leptin. To determine the mechanism, groups of mice were respectively fed a diet containing H. erinaceus powder, a diet containing ethanol extract of H. erinaceus, and a diet containing a residue of the extract. As a result, H. erinaceus powder was found to increase fecal lipid levels in excreted matter. Further in vitro investigation showed that ethanol extract inhibited the activity of lipase, and four lipase-inhibitory compounds were isolated from the extract: hericenone C, hericenone D, hericenone F, and hericenone G. In short, we suggest that H. erinaceus has an anti-obesity effect during menopause because it decreases the ability to absorb lipids..
21. Bungo Shirouchi, Kentaro Kashima, Yasutaka Horiuchi, Yuki Nakamura, Yumiko Fujimoto, Li-Tao Tong, MASAO SATO, 27-Hydroxycholesterol suppresses lipid accumulation by down-regulating lipogenic and adipogenic gene expression in 3T3-L1 cells, Cytotechnology, 10.1007/s10616-016-9962-5, 69, 3, 485-492, 2017.06.
22. Kazuo ERAMI, Yasutake TANAKA, Sayaka KAWAMURA, Motonori MIYAGO, Ai SAWAZAKI, katsumi imaizumi, MASAO SATO, Dietary Egg Yolk Supplementation Improves Low-Protein-Diet-Induced Fatty Liver in Rats, Journal of Nutritional Science and Vitaminology, 10.3177/jnsv.62.240, 62, 4, 240-248, 2016.08, [URL], Egg yolk is an important source of nutrients and contains different bioactive substances. In the present study, we studied the benefits of egg yolk in preventing low-protein-diet-induced fatty liver in rats. Rats were fed the following diets, which were based on the AIN-76 formula, for 2 wk: an adequate-protein diet containing 20% casein (C), a low-protein diet containing 5% casein (LP-C), a low-protein diet supplemented with 12.5% egg yolk (LP-EY), and a low-protein diet supplemented with 4.1% egg yolk oil (LP-EYO). The low-protein diets were adjusted to contain 4.13% protein and 4.7% lipids. The LP-C diet resulted in a greater increase in the liver trigriceride (TG) and the vacuolation and a greater decrease in the serum TG and free fatty acid (FFA) than did the C diet. These deviations in the serum and liver TG, serum FFA levels and the liver histopathology were corrected in rats fed the LP-EY diet but not in those fed the LP-EYO diet. Compared to rats fed the LP-C diet, although the activities of lipogenesis-related enzymes (fatty acid synthase, glucose-6-phosphate dehydrogenase, and malic enzyme) decreased in rats fed both of the LP-EY and LP-EYO diets, the level of the microsomal TG transfer protein (MTP) increased only in rats fed the LP-EY diet. Collectively, these results suggest that dietary egg yolk supplementation decreases the LP diet-induced accumulation of TG in the liver by increasing transport of TG in the liver, and egg yolk oil alone is not sufficient enough to bring about these benefits..
23. Bungo Shirouchi, Kouji Nagao, Minami Umegatani, Aya Shiraishi, Yukiko Morita, Shunichi Kai, Teruo Yanagita, Akihiro Ogawa, Yukio Kadooka, MASAO SATO,  Probiotic Lactobacillus gasseri SBT2055 improves glucose tolerance and reduces body weight gain in rats by stimulating energy expenditure., British Journal of Nutrition, 116, 451-458, 2016.05.
24. Sakata M, Seno M, Matsusaka H, Takahashi K, Nakamura Y, Yamagata Y, Angeles ER, Mochizuki T, Kumamaru T, Sato M, Enomoto A, Tashiro K, Kuhara S, Satoh H, Yoshimura A, Development and evaluation of rice giant embryo mutants for high oil content originated from a high-yielding cultivar ‘Mizuhochikara’, Breeding Science, 66, 425-443, 2016.03.
25. Shigeru Murakami, Michiko Fujita, Masakazu Nakamura, Masanobu Sakono, Shoko Nishizono, Masao Sato, Katsumi Imaizumi, Mari Mori, Nobuhiro Fukuda, Taurine ameliorates cholesterol metabolism by stimulating bile acid production in high-cholesterol-fed rats, Clinical and Experimental Pharmacology and Physiology, 10.1111/1440-1681.12534, 43, 3, 372-378, 2016.03, © 2016 John Wiley & Sons Australia, Ltd. Summary: This study was designed to investigate the effects of dietary taurine on cholesterol metabolism in high-cholesterol-fed rats. Male Sprague-Dawley rats were randomly divided into two dietary groups (n = 6 in each group): a high-cholesterol diet containing 0.5% cholesterol and 0.15% sodium cholate, and a high-cholesterol diet with 5% (w/w) taurine. The experimental diets were given for 2 weeks. Taurine supplementation reduced the serum and hepatic cholesterol levels by 37% and 32%, respectively. Faecal excretion of bile acids was significantly increased in taurine-treated rats, compared with untreated rats. Biliary bile acid concentrations were also increased by taurine. Taurine supplementation increased taurine-conjugated bile acids by 61% and decreased glycine-conjugated bile acids by 53%, resulting in a significant decrease in the glycine/taurine (G/T) ratio. Among the taurine-conjugated bile acids, cholic acid and deoxycholic acid were significantly increased. In the liver, taurine supplementation increased the mRNA expression and enzymatic activity of hepatic cholesterol 7α-hydroxylase (CYP7A1), the rate-limiting enzyme for bile acid synthesis, by three- and two-fold, respectively. Taurine also decreased the enzymatic activity of acyl-CoA:cholesterol acyltransferase (ACAT) and microsomal triglyceride transfer protein (MTP). These observations suggest that taurine supplementation increases the synthesis and excretion of taurine-conjugated bile acids and stimulates the catabolism of cholesterol to bile acid by elevating the expression and activity of CYP7A1. This may reduce cholesterol esterification and lipoprotein assembly for very low density lipoprotein (VLDL) secretion, leading to reductions in the serum and hepatic cholesterol levels..
26. Kaori Tsuiki, Haruka Fujisawa, Ayaka Itoh, MASAO SATO, Naoko Fujita, Alterations of starch structure lead to increased resistant starch of steamed rice: Identification of high resistant starch rice lines, Journal of Cereal Science, 68, 88-92, 2016.01, The development of low calorie foods to aid obesity control is a growing area of research. Among these,
resistant starch (RS) in cereals has beneficial effects on reducing colon cancer rate and better fatty acid
composition as well as lowering calories. Rice contains digestible starch and indigestible RS, the proportions
of which are affected by starch biosynthetic enzymes. Rice lines that carry mutations in starch
biosynthesis genes may have high levels of RS. In this study, percentage RS and non-digested component
values were determined in steamed rice from several rice lines. These include several high-amylose
indica rice cultivars, and lines carrying mutations in the starch synthase (SS) IIIa and/or branching
enzyme (BE) IIb genes. High RS rice lines contained high levels of non-digested component. RS
values 4% correlated with apparent amylose content. RS values of BEIIb-deficient mutant lines were
particularly high (15 RS 35%), and were correlated with the amount of amylopectin long chains.
Among rice lines used in this study, be2b mutant lines having large portions of amylopectin long chains
were the best candidates for high RS rice foods.
27. Ikuko Kashino, Akiko Nanri, Kayo Kurotani, Shamima Akter, Kazuki Yasuda, MASAO SATO, Hitomi Hayabuchi, Tetsuya Mizoue, Association of dietary patterns with serum adipokines among Japanese: a cross-sectional study
, Nutrition Journal, 10.1186/s12937-015-0046-8., 14, 58, 2015.08.
28. Y. Kimura, Ngoc Minh Phama, Kazuki Yasuda, Akiko Nanri, Kayo Kurotani, Keisuke Kuwahara, Shamima Akter, MASAO SATO, H. Hayabuti, Tetsuya Mizoue, Association of adulthood weight gain with circulating adipokine and insulin resistance in the Japanese population, European Journal of Clinical Nutrition, 10.1038/ejcn.2014.257, 69, 462-466, 2015.04, Background/Objectives:Although adipokines and insulin resistance are known to be correlated with body fatness, it is unclear whether they are independently related to weight gain experience. We examined the associations of serum adipokines and marker of insulin resistance with past weight gain during adulthood by taking the degree of attained body mass index (BMI) level into consideration.Subjects/Methods:Subjects were 399 Japanese municipal employees, aged ⩾30 years, who participated in a health survey. Serum adipokines were measured using a Luminex suspension bead-based multiplexed array. Weight change during adulthood was calculated as the difference between measured current weight and recalled weight at the age of 20 years. Multiple regression was performed to calculate mean adipokine levels and homeostasis model assessment of insulin resistance (HOMA-IR) according to weight gain (<5 kg, 5–9.9 kg, or ⩾10 kg) with adjustment for current BMI.Results:Weight gain from the age of 20 years was significantly and positively associated with leptin levels even after adjustment for current BMI (P for trend <0.001), whereas it was significantly and inversely associated with adiponectin levels in a BMI-adjusted model among subjects aged ⩾40 years (P for trend=0.03). Weight gain was associated with HOMA-IR in a BMI-unadjusted model (P for trend <0.001), but this association was largely attenuated after adjustment for BMI. Resistin, plasminogen activator inhibitor-1 and visfatin were not associated with past weight gain.Conclusions:Results suggest that a large weight gain during adulthood is associated with higher leptin and lower adiponectin levels independently of the degree of attained BMI level.European Journal of Clinical Nutrition advance online publication, 3 December 2014; doi:10.1038/ejcn.2014.257. © 2014 Macmillan Publishers Limited.
29. Ngoc Minh Phama, Akiko Nanri, Siyan Yi, Kayo Kurotani,, Shamima Akter, Leng Huat Foo, Nobuo Nishi, MASAO SATO, Hitomi Hayabuchi, Tetsuya Mizoue, Habitual consumption of coffee and green tea in relation to serum adipokines: a cross-sectional study, European Journal of Nutrition , 10.1007/s00394-014-0701-4, 54, 2, 205-214, 2015.03, PURPOSE:Coffee and green tea consumption may be associated with circulating adipokines, but data are inconsistent, scarce or lacking. We examined the association of coffee and green tea consumption with serum adiponectin, leptin, visfatin, resistin and plasminogen activator inhibitor-1 (PAI-1) among a Japanese working population.
The authors analyzed data (n = 509) from a cross-sectional survey among Japanese workers aged 20-68 years. Serum adipokines were measured using a Luminex suspension bead-based multiplexed array. Coffee and green tea consumption was assessed using a validated diet history questionnaire, and caffeine consumption from these beverages was estimated. Multiple regression analysis was performed with adjustment for potential confounding variables.
Coffee consumption was significantly, inversely associated with leptin and PAI-1 (P for trend = 0.007 and 0.02, respectively); compared with subjects consuming <1 cup per day, those consuming ≥4 cups per day had 13 and 10 % lower means of leptin and PAI-1, respectively. Similar associations were observed for caffeine consumption (P for trend = 0.02 for both leptin and PAI-1). Additionally, we noted a significant positive association between coffee consumption and adiponectin in men (P for trend = 0.046), but not in women (P for trend = 0.43, P for interaction = 0.11). Moreover, there was a positive association between coffee consumption and resistin in current male smokers (P for trend = 0.01), but not in male non-smokers (P for trend = 0.35, P for interaction = 0.11). Green tea consumption was not associated with any adipokine.
Higher consumption of coffee and caffeine but not green tea was associated with lower serum levels of leptin and PAI-1 in Japanese adults.
30. Chisato Inoue, Tomomi Kozaki, Yukiko Morita, Bungo Shirouchi, Katsuya Fukami, kuniyoshi shimizu, MASAO SATO, Yoshinori Katakura, Kibizu concentrated liquid suppresses the accumulation of lipid droplets in 3T3-L1 cells, Cytotechnology, 2015.02, Adipocyte size is closely related to the occurrence of diabetes, metabolic syndrome, and insulin resistance. Thus, researchers are searching for active substances that function to reduce adipocyte size. In the present study, we focused on sugar cane vinegar, Kibizu, and evaluated the function of Kibizu to reduce adipocyte size by using an in vitro model system, because people in Amami Oshima famous for longevity regularly consume Kibizu. Results showed that Kibizu treatment significantly reduced the size and number of lipid droplets in 3T3-L1 cells, relative to treatment with Kurozu, another traditional vinegar. Results of an extraction experiment suggest that the active components in Kibizu are lipophilic and hydrophobic. In addition, an in vivo experiment on rats treated with Kibizu showed that the active components were contained in large vein blood. Results of an additional in vivo experiment suggest that metabolites generated by Kibizu-treated rats are primarily contained or modified specifically in the large vein blood..
31. Ryousuke Matsuoka, Bungo Shirouchi, Masao Sato, Sayaka Kawamura, Sanae Baba, Sawako Shiratake, Kazuko Nagata, Katsumi Imaizumi, Dietary Egg White Protein Inhibits Lymphatic Lipid Transport in Thoracic Lymph Duct-Cannulated Rats, Journal of Agricultural and Food Chemistry, 10.1021/jf502741b, 62, 44, 10694-10700, 2014.11, Dietary egg white protein (EWP) decreases serum cholesterol levels. We previously showed that EWP decreased cholesterol absorption in the intestine. Rats subjected to permanent lymph duct cannulation were used to investigate the effects of dietary EWP on lipid transport. They were fed diets with 20% EWP and casein, and their lymph was collected to quantify lymphatic lipid levels. Dietary EWP decreased lymphatic cholesterol transport compared with casein. It was previously shown that EWP excluded cholesterol from bile acid micelles. Therefore, pepsin-hydrolyzed EWP and casein were prepared. EWP was not completely digested. Ovalbumin, which is the most abundant protein in EWP, showed resistance to digestion by pepsin. This study investigated the effects of EWP pepsin hydrolysate (EWP-ph) on cholesterol micellar solubility, cholesterol transfer from the micellar to the oil phase, water-holding capacity (WHC), settling volume in water (SV), and relative viscosity and compared them with the effects of casein pepsin hydrolysate (C-ph). EWP-ph significantly decreased the micellar solubility and transfer rate and increased the WHC, SV, and relative viscosity compared with C-ph. Moreover, the pepsin hydrolysate of ovalbumin, a major protein in EWP, played a role in decreasing cholesterol micellar solubility, leading to the inhibition of cholesterol absorption. In conclusion, dietary EWP decreased cholesterol intestinal absorption by exerting combined effects of these physicochemical properties in the gut..
32. Ai Hori, Hiroshi Kasai, Kazuaki Kawai, Akiko Nanri, MASAO SATO, Masanori Ohta, Tetsuya Mizoue, COFEE INTAKE IS ASSOCIATED WITH REDUCING OXIDATIVE DNA DAMAGE AND DECREASING BODY IRON STORAGE IN HEALTHY WOMEN, Nutrition and Cancer , 10.1080/01635581.2014.932398., 66, 6, 964-969, 2014.06, Habitual coffee drinking has been linked to a lower risk for some forms of cancer, but the mechanism remains elusive. Coffee may decrease oxidative DNA damage, an important pathway to carcinogenesis. We examined the association between coffee consumption and urinary 8-hydroxydeoxyguanosine (8-OHdG) concentrations, a biomarker of systemic oxidative DNA damage and repair, in 507 healthy subjects (298 men and 209 women aged 21-67 years) while adjusting for age, sex, smoking status, body mass index, job type, and fasting blood glucose in multivariable regression models. The association with green tea consumption was also assessed. Urinary 8-OHdG concentrations tended to decrease with coffee consumption (Trend P=0.068) only in women, with participants drinking 2-3 cups of coffee per day showing the lowest mean of urinary 8-OHdG concentrations. This association diminished after adjustment for serum ferritin concentrations, a marker of body iron storage (Trend P=0.87). Green tea consumption was not significantly associated with urinary 8-OHdG concentrations. Coffee drinking may be associated with decreased systemic oxidative DNA damage through decreasing body iron storage in women..
33. Hiroyuki Kikuchi, Akiko Nanri, Ai Hori, MASAO SATO, Kazuaki Kawai, Hiroshi Kasai, Tetsuya Mizoue, Lower serum levels of total cholesterol are associated with higher urinary levels of 8-hydroxydeoxyguanosine, Nutirition and Metabolism, 10.1186/10.1186/1743-7075-10-59, 10, Art. NO. 59, 2014.03, [URL], Background
Lower serum total (TC), high-density lipoprotein (HDL-C) and low-density lipoprotein cholesterols (LDL-C) have been linked to an increased risk of cancer in various sites, but its underlying mechanism remains unclear. In an attempt to clarify the association between cholesterol levels and oxidative DNA damage, we investigated the relationship between serum cholesterol and urinary 8-hydroxydeoxyguanosine levels in a Japanese working population.
The study subjects were 294 men and 209 women aged 21-66 years in two Japanese municipal offices. Urinary 8-hydroxydeoxyguanosine (8-OHdG) was measured using an automated high-pressure liquid chromatography. Linear regression analysis was used to examine the associations of urinary 8-OHdG with TC, HDL-C and LDL-C levels with adjustment for sex, age, smoking and body mass index. Subgroup analyses were conducted by smoking status in men and age in women. Analysis of covariance was employed to estimate adjusted means of urinary 8-OHdG across TC category.
After multivariate adjustment, urinary 8-OHdG levels were inversely associated with serum TC levels (β = −0.0015, p < 0.05) and LDL-C levels (β = −0.0012, p = 0.07). The inverse association with TC was apparent among smoking men (β = −0.0017, p < 0.05) and among women aged less than 48 years (β = −0.0040, p < 0.01). 8-OHdG decreased as TC increased (up to 219 mg/dL); subjects with TC levels of <160 mg/dL had a 17.4% higher adjusted mean of 8-OHdG than did those with TC levels of 200–219 mg/dL.
Results suggest that circulating low TC levels are associated with higher oxidative DNA damage.
34. Ngoc Minh Pham, Akiko Nanri, Takeshi Kochi, Keisuke Kuwahara, Hiroko Tsuruoka, Kayo Kurotani, Shamima Akter, Isamu Kabe, MASAO SATO, Hitomi Hayabuchi, Tetsuya Mizoue, Coffee and green tea consumption is associated with insulin resistance in Japanese adults, Metabolism - Clinical and Experimental, 10.1016/j.metabol.2013.11.008, 63, 3, 400-408, 2014.03, Objective. Higher coffee and green tea consumption has been suggested to decrease risk of type 2 diabetes, but their roles in insulin resistance (IR) and insulin secretion remain unclear. This study examined the association between habitual consumption of these beverages with markers of glucose metabolism in a Japanese working population.
Materials/Methods. Participants were 1,440 Japanese employees (1,151 men and 289 women) aged 18-69 years. Consumption of coffee and green tea was ascertained via a validated brief diet history questionnaire. Multilevel linear regression was used to estimate means and 95% confidence intervals of fasting insulin, fasting plasma glucose, homeostatic model assessment of IR (HOMA-IR), homeostatic model assessment of β-cell function (HOMA-β) and glycated hemoglobin (HbA1c) with adjustment for potential confounding variables. Stratified analyses by smoking and body mass index (BMI) were also performed.
Results. Coffee consumption was significantly, inversely associated with HOMA-IR (P for trend = .03), and the association appeared to be confined to overweight subjects (BMI ≥25kg/m2 ) (P for trend = .01, P for interaction = .08). Contrarily, there was a significant, positive association between green tea consumption and HOMA-IR (P for trend = .015), though dose response relationship among daily consumers of green tea was not observed. Neither coffee nor green tea consumption was associated with HOMA-β and HbA1c.
Conclusions. Our findings indicate that coffee consumption may be associated with decreased IR, but not with insulin secretion. The positive association between green tea consumption and IR warrants further investigation..
35. Kayo Kurotani, MASAO SATO, Yuko Ejima, Kentaro Kashima, Akiko Nanri, Ngoc Minh Pham, Keisuke Kuwahara, Tetsuya Mizoue, Serum alpha-linolenic and linoleic acids are inversely associated with depressive symptoms in adults, e-SPEN Journal, 10.1016/j.clnme.2013.12.003, 9, 1, e7-e12, 2014.02, Background & Aims: Fatty acid composition in diet and serum has been linked to depression, but the evidence on this issue is limited among Japanese, who consume large amounts of fish rich in n-3 polyunsaturated fatty acids. We examined the association between fatty acid composition in serum and depressive symptoms in Japanese men and women.
Methods: The subjects were 496 participants aged 21-67 years in a cross-sectional study. Depressive symptoms were assessed using the Center for Epidemiologic Studies Depression (CES-D) scale. Free, cholesterol ester, and phospholipid fatty acids in serum were measured by gas-liquid chromatography. Multiple logistic regression was used to assess the association between serum fatty acid and depressive symptoms.
Results: A higher free alpha-linolenic acid level was marginally significantly associated with a decreased prevalence of depressive symptoms (CES-D≥16) (P for trend = 0.07). When a cut-off of CES-D≥19 was used, the association was strengthened; the multivariable-adjusted odds ratios (95% confidence interval) of depressive symptoms for the lowest through highest tertile of alpha-linolenic acid levels were 1.00 (reference), 0.49 (0.29-0.84), and 0.47 (0.26-0.83), respectively (P for trend = 0.007). A higher n-6 PUFA in cholesterol esters and free linoleic acid were also significantly associated with a decreased prevalence of depressive symptoms (CES-D≥19) (P for trend = 0.03 and 0.048, respectively). The other polyunsaturated fatty acids including marine-derived n-3 PUFA were not associated with depressive symptoms.
Conclusions: Fatty acid composition with high levels of serum alpha-linolenic and linoleic acids might be protectively associated with depressive symptoms in Japanese adults.
36. Akihiro Ogawa, Yukio Kadooka, Ken Kato, Bungo Shirouchi, MASAO SATO, Lactobacillus gasseri SBT2055 reduces postprandial and fasting serum non-esterified fatty acid levels in Japanese hypertriacylglycerolemic subjects, Lipids in Health and Disease, 19, 13(1), 36, 2014.02.
37. Bungo Shirouchi, Elke Albrecht, Gerd Nuernberg, Steffen Maak, Samadmanivong Olavanh, Yoshinori Nakamura, MASAO SATO, Takaufmi Gotoh, Karin Nuernberg, Fatty acid profiles and adipogenic gene expression of various fat depots in Japanese Black and Holstein steers, Meat Science, 10.1016/j.meatsci.2013.06.027., 96, 1, 157-164, 2014.01, Objective of the study was to assess the breed effect on fatty acid (FA) composition of different adipose tissues and on mRNA expression of genes involved in adipogenesis and fat metabolism. Japanese Black (JB) and Holstein (HS) steers were kept under equivalent conditions with high energy intake resulting in large differences in intramuscular fat (IMF) accumulation in longissimus muscle (LM). The relative FA composition of muscle, intermuscular fat, visceral fat, and perirenal fat was comparable between JB and HS steers. Circulating fatty acids were also similar in both breeds. Most relevant breed effects were identified in IMF, underlining the uniqueness of this adipose tissue site. JB steers had more monounsaturated FA and less saturated FA. Perilipin 1 and adipose differentiation-related protein (ADFP) mRNA levels were higher in IMF of JB. The results suggest advanced maturity of IMF cells in JB and altered local conditions in muscle influencing IMF accumulation and composition.
38. Yasutake Tanaka, Koji Nagao, Hideaki Nakagiri, Toshirou Nagaso, Yasue Iwasa, Haruhiko Mori, Makoto Asahina, Katsumi Imaizumi, Masao Sato, Unavailability of liver triacylglycerol increases serum cholesterol concentration induced by dietary cholesterol in exogenously hypercholesterolemic (ExHC) rats, Lipids in Health and Disease, 10.1186/1476-511X-13-19, 13, 1, 13-19, 2014.01, Background: Exogenously hypercholesterolemic (ExHC) rats develop hypercholesterolemia and low hepatic triacylglycerol (TAG) levels when dietary cholesterol is loaded. The responsible gene Smek2 was identified via linkage analysis using the original strain Sprague-Dawley (SD) rats. In this study, we compared SD and ExHC rats to investigate a relationship between hypercholesterolemia and the low hepatic TAG levels observed in ExHC rats. Methods. Male 4-weeks-old ExHC and SD rats were fed a 1% cholesterol diet for 1 week. Serum and liver parameters were analyzed. Gene expression and enzyme activities related to TAG metabolism were also assessed. Results: We reproducibly observed higher serum cholesterol and lower hepatic TAG levels in ExHC rats than in SD rats. Golgi apparatus in the livers of ExHC rats secreted β-very-low-density lipoprotein (β-VLDL) that had higher cholesterol ester (CE) and lower TAG content than those in the β-VLDL secreted by SD rats. Gene expression related to fatty acid and TAG synthesis in ExHC rats was lower than that in SD rats. Enzymatic activities for fatty acid synthesis were also relatively lower in ExHC rats. Moreover, the fatty acid composition of hepatic and serum CE in ExHC rats showed that these CEs were not modified after secretion from the liver despite the similar activities of serum lecithin-cholesterol acyltransferase (LCAT) in ExHC rats to those in SD rats. Conclusions: Low production of liver TAG and secretion of CE-rich, TAG-poor β-VLDL without modification by LCAT in the circulation contributed to hypercholesterolemia induced by dietary cholesterol in ExHC rats. © 2014 Tanaka et al.; licensee BioMed Central Ltd..
39. Masako Iwamoto, kaori Yagi, Kayoko Yazum, Airi Komine, MASAO SATO, Bungo Shirouchi, Eating a healthy lunch improves serum alanine aminotransferase activity, Lipids in Health and Disease, 10.1186/1476-511X-12-134, 12, 134, 2013.09, Nutritional guidance and diet control play important roles in the treatment of obesity and non-alcoholic fatty liver. However, in Japan, nutritional guidance is difficult to provide in practice. Therefore, we evaluated the effects of providing the 'once-a-day' intervention of a healthy lunch on various metabolic parameters.
For a 1-month preparatory period, 10 subjects generally consumed the lunches that were provided by the worksite cafeteria. This was followed by a 1-week washout period, after which, the subjects consumed healthy, low-calorie, well-balanced lunches for a 1-month test period. After the preparatory and test periods, blood samples were obtained from all subjects. The serum levels of indices relevant to metabolic syndrome and fatty liver were measured.
Serum alanine aminotransferase activity significantly decreased by 20.3% after the healthy intervention. However, the indices of metabolic syndrome did not significantly change. Analysis of the relationship between serum alanine aminotransferase activity and nutrient content indicated that the improvement of serum alanine aminotransferase status was due to the higher vegetable content and lower animal-source protein of the meals provided.
In summary, the 'once-a-day' intervention of providing a healthy lunch improved serum alanine aminotransferase status. A diet high in vegetables and low in animal-based protein is important in maintaining a healthy condition.
40. Ayami Kume, Kayo Kurotani, MASAO SATO, Yuko Ejima, Ngoc Minh Pham, Akiko Nanri, Keisuke Kuwahara, Tetsuya Mizoue, Polyunsaturated fatty acids in serum and homocysteine concentrations in Japanese men and women: a cross-sectional study, Nutrition & Metabolism, 10.1186/1743-7075-10-41, 10, 1, Article NO. 41, 2013.07, Background: Supplementation studies have suggested a role of n-3 polyunsaturated fatty acids
(PUFAs) in homocysteine metabolism, but the evidence is limited and inconsistent among studies
that measured blood levels of n-3 and n-6 PUFAs. We examined the association between blood
levels of PUFAs and homocysteine in Japanese men and women.
Methods: The subjects were 496 employees (290 men and 206 women) of 2 municipal offices in
Japan. Fatty acid composition in serum phospholipids and cholesterol ester (CE) was measured
using gas-liquid chromatography. Multiple regression was used to calculate means of homocysteine
concentrations according to PUFA tertile with adjustment for potential confounders.
Results: Serum homocysteine concentration decreased with increasing levels of total n-3 PUFA,
eicosapentaenoic acid and docosahexaenoic acid (DHA) in serum phospholipids and CE with
adjustment for age, sex and workplace. However, only DHA in serum phospholipids remained
statistically significant after additional adjustment for other potential confounders including serum
folate (P-trend = 0.04). N-6 PUFAs were not significantly associated with homocysteine
Conclusions: Higher proportion of DHA in serum phospholipids may be associated with lower
homocysteine concentrations in Japanese men and women..
41. Akiko Nanri, Minh N Pham, Kayo Kurotani, Ayami Kume, Keisuke Kuwahara, MASAO SATO, Hitomi Hayabuchi, Tetsuya Mizoue, Serum pyridoxal concentrations and depressive symptoms among Japanese adults: results from a prospective study, European Journal of Clinical Nutrition, 10.1038/ejcn.2013.115, PMID: 23801094, 2013.07, BACKGROUND/OBJECTIVE: Vitamin B6 is suggested to play a protective role against depression. However, the association between vitamin B6 intake and depression remains inconclusive, and few studies have examined the relationship between circulating vitamin B6 concentrations and depressive symptoms. Here, we investigated the cross-sectional and prospective associations between serum pyridoxal concentrations and depressive symptoms among Japanese workers.
SUBJECTS/METHODS: Participants were 422 municipal employees (aged 21-67 years) who participated in a baseline survey in 2006 for cross-sectional analysis, and 210 subjects without depressive symptoms at baseline (2006) who completed both baseline and follow-up (2009) surveys for prospective analysis. Depressive symptoms were assessed using the Center for Epidemiologic Studies Depression (CES-D) scale. Logistic regression analysis was used to estimate the odds ratio of depressive symptoms (CES-D scale of ≥19) according to tertile of serum pyridoxal with adjustment for potential confounding variables.
RESULTS: In the cross-sectional analysis, serum pyridoxal concentrations were significantly associated with a decreased prevalence of depressive symptoms (P for trend = 0.03); the multivariable-adjusted odds ratio of depressive symptoms for the highest tertile of pyridoxal was 0.54 (95% confidence interval 0.30-0.96) compared with the lowest tertile. In longitudinal analyses, higher serum pyridoxal concentrations at baseline were associated with a trend towards reduced depressive symptoms after three years; the multivariable-adjusted odds ratio of depressive symptoms for the highest versus the lowest tertile of pyridoxal concentration was 0.55 (95% confidence interval 0.13-2.32).
CONCLUSIONS: A higher vitamin B6 status may be associated with a decreased risk of depressive symptoms in Japanese..
42. Keisuke Kuwahara, Akiko Nanri, Minh N Pham, MASAO SATO, Kayo Kurotani, Kazuaki Kawai, Hiroshi Kasai, Tetsuya Mizoue, Serum vitamin B6, folate, and homocysteine concentrations and oxidative DNA damage in Japanese men and women
, Nutrition, 10.1016/j.nut.2013.03.014, 29, 10, 1219-1223, 2013.07.
43. Ohn Mar Khin, MASAO SATO, Tong Li-Tao, Matsue, Y., Atsushi Yoshimura, Toshihiro MOCHIZUKI, Close association between aleurone traits and lipid contents of rice grains observed in widely different genetic resources of Oryza sativa, Plant Production Science, 10.1626/pps.16.41, 16, 1, 41-49, 2013.01, To identify the useful genetic resources for aleurone traits that are closely related to the lipid content of rice, we investigated 321 varieties. Brown rice seeds were cut in crosswise near the center with razor blade and the cut surface was stained with Oil Red O, and then observed under integrated fluorescence microscope (BZ9000). We found wide variations among varieties in the area stained, but the japonica group contained many varieties with large stained area. We selected 17 varieties to cover the whole range of stained areas among varieties tested an confirmed the thickness of aleurone layer using Kawamoto’s film method (micro-thin sections that were created with cryomicrotome). Aleurone traits were examined under a light microscope and measured. We found a strong correlation between the area of the aleurone layer and the stained area (r = 0.799***) and between the thickness of aleurone layer and the thickness of stained region (r = 0.543*). The area of the stained region therefore provides a fast and effective indicator for selecting varieties for the thickness of the aleurone layer. We also measured the amount (mg per 100 seeds) and proportion of triacylglycerols (TAG) to the 1 g of seed (mg g-1) in brown rice seeds without embryos. The amount and proportion of TAG to the 1 g of seed were both significantly correlated with the area, average thickness and percentage of aleurone layer. This suggests that the aleurone traits will provide good indicators for the selection of
varieties with high levels of TAG..
44. Ngoc Minh Pham, Shamima Akter, Kayo Kurotani, Akiko Nanri, MASAO SATO, Hitomi Hayabuchi, Kazuki Yasuda, Tetsuya Mizoue, Serum 25-hydroxyvitamin D and markers of insulin resistance in a Japanese working population, European Journal of Clinical Nutrition, 10.1038/ejcn.2012.169, 66, 12, 1323-1328, PMID: 23093338, 2012.12, Background/Objectives:The association between vitamin D status and insulin resistance (IR) has been less studied among Asians, and it remains elusive whether calcium could modify such an association. We examined the association of serum 25-hydroxyvitamin D (25(OH)D) concentrations with IR markers and the potential effect modification by calcium intake among a Japanese population.
Subjects/Methods:The authors analyzed data (n=494) from a cross-sectional survey conducted in 2009 among a Japanese working population aged 20–68 years. Fasting serum 25(OH)D and insulin, as well as fasting plasma glucose were determined, and homeostatic model assessment of IR (HOMA-IR) was calculated. Calcium intake was assessed using a validated diet history questionnaire. Multiple linear regression was done with adjustment of potential confounding variables.
Results:Fasting insulin and HOMA-IR were significantly inversely associated with 25(OH)D concentration across quartiles of 25(OH)D after fully adjusting for covariates (Ptrend=0.04 and 0.02, respectively). Across clinically relevant categories of 25(OH)D, compared with participants in the vitamin D sufficiency group, those in the vitamin D insufficiency group had a 5% higher HOMA-IR score, and those in the hypovitaminosis D group had an 18% higher HOMA-IR score (Ptrend=0.01). In an analysis by calcium intake, the HOMA-IR score was highest among participants with both a low calcium intake and lowest 25(OH)D concentrations, with significant inverse trend being observed in the group with lower calcium intake (Ptrend=0.02).
Conclusions:Our findings suggest that low vitamin D status is associated with IR among Japanese adults..
45. Akiko Nanri, Hitomi Hayabuchi, Masanori Ohta, MASAO SATO, Norio Mishima, Tetsuya Mizoue, Serum folate and depressive symptoms among Japanese men and women: a cross-sectional and prospective study, Psychiatry Research, 10.1016/j.psychres.2012.04.040, 200, 2-3, 349-353, PubMed ID: 22682152, 2012.12, Although several studies have reported an association between blood folate concentrations and depressive symptoms, few studies have prospectively examined the association. This study aimed to investigate the cross-sectional and prospective associations between serum folate concentrations and depressive symptoms among Japanese. We analyzed data among 545 subjects who participated in a health survey at the time of periodic checkup in 2009 for cross-sectional association and among 272 subjects without depressive symptoms at baseline (in 2006) who responded to both baseline (2006) and follow-up (2009) surveys for prospective association. Depressive symptoms were assessed using the Center for Epidemiologic Studies Depression (CES-D) scale. In a cross-sectional analysis, serum folate concentrations were significantly associated with a decreased prevalence of depressive symptoms (CES-D scale of ≥16). Moreover, serum folate concentrations at baseline were significantly inversely associated with depressive symptoms after three years; the multivariate-adjusted odds ratios (95% confidence intervals) of depressive symptoms for the lowest through highest tertile categories of serum folate concentrations were 1.00 (reference), 0.66 (0.29-1.52), and 0.40 (0.16-0.99) (P for trend = 0.047). Our findings suggest that higher serum folate may be associated with decreased risk of depressive symptoms in Japanese..
46. Bungo Shirouchi, Nakamura Y, Furukawa Y, Shiraishi A, Tomoyori H, katsumi imaizumi, MASAO SATO, Ezetimibe inhibits lymphatic transport of esterified cholesterol but not free cholesterol in thoracic lymph duct-cannulated rats., Cardiovascular Drugs and Therapy, 10.1007/s10557-012-6403-3, 26, 5, 427-431, 2012.10, [URL], Purpose Ezetimibe has been shown to inhibit dietary choles- terol absorption in animal models and humans, but studies on lymphatic lipid transport have not yet been performed. Rats subjected to permanent lymph duct cannulation were used to investigate the effects of ezetimibe on lipid transport. Methods Rats were fed diets with and without ezetimibe (5.0 mg/kg), and their lymph was collected after feeding to quantify lymphatic lipid levels. Total cholesterol content in the intestinal mucosa was also measured.
Results Rats that consumed ezetimibe had significantly lower lymphatic total cholesterol transport with the reduction of esterified cholesterol transport. According to the calculation based on cholesterol consumption, ezetimibe reduced the total cholesterol lymphatic recovery rate by 54 %. We also deter- mined that ezetimibe significantly reduced the total cholester- ol content in the intestinal mucosa.
Conclusion This is the first direct evidence that ezetimibe inhibits esterified but not free cholesterol lymphatic transport in thoracic duct-cannulated rats. The results also indicate that ezetimibe is not involved in the lymphatic transport of triacyl- glycerols, phospholipids, or α-tocopherol..
47. Masako Iwamoto, Katsumi Imai, Hideaki Ohta, Bungo Shirouch and Masao Sato, Supplementation of highly concentrated beta-cryptoxanthin in a satsuma mandarin beverage improves adipocytokine profiles in obese Japanese women, Lipids in Health and Disease, 10.1186/1476-511X-11-52, 11, 1, 52, 2012.09, Background: Serum β-cryptoxanthin levels are lower in overweight subjects than in normal subjects. Abnormalities of adipocytokine profiles in obesity subjects have been reported. There are several reports that serum β-cryptoxanthin levels in them were relatively lower than normal subjects.
Objective: We hypothesize that supplementation of highly concentrated β-cryptoxanthin improves serum adipocytokine profiles in obese subjects. This study tested the association between β-cryptoxanthin intake and serum adipocytokine levels.
Methods: An intervention study consisted of a 3-week long before-and-after controlled trial, where β-cryptoxanthin (4.7 mg/day) was given to 17 moderately obese postmenopausal women.
Results: The results indicated no significant changes in body weight or body mass index (BMI). Serum β-cryptoxanthin levels increased significantly by 4-fold. Serum high molecular weight (HMW)-adiponectin levels increased significantly, while serum plasminogen activator inhibitor (PAI)-1 levels decreased.
Conclusions: We concluded that increasing the intake of β-cryptoxanthin to approximately 4 mg per day for 3 weeks may have beneficial effects on the serum adipocytokine status and consequently alleviate progression of metabolic syndrome.
48. Kayo Kurotani, Masao Sato, Yuko Ejima, Akiko Nanri, Siyan Yi, Ngoc Minh Pham, Shamima Akter, Kalpana Poudel-Tandukar, Yasumi Kimura, Katsumi Imaizumi, Tetsuya Mizoue, High levels of stearic acid, palmitoleic acid, and dihomo-γ-linolenic acid and low levels of linoleic acid in serum cholesterol ester are associated with high insulin resistance, Nutrition Research, 10.1016/j.nutres.2012.07.004, 32, 9, 669-675, 2012.09, The association of fatty acid composition with insulin resistance and type 2 diabetes has been reported in Western populations, but there is limited evidence of this association among the Japanese, whose populace consume large amounts of fish. To test the hypothesis that high palmitic, palmitoleic, and dihomo-γ-linolenic acids and low levels of linoleic and n-3 fatty acids are associated with higher insulin resistance among the Japanese, the authors investigated the relationship between serum fatty acid composition and serum C-peptide concentrations in 437 Japanese employees aged 21 to 67 years who participated in a workplace health examination. Serum cholesterol ester and phospholipid fatty acid compositions were measured by gas-liquid chromatography. Desaturase activity was estimated by fatty acid product-to-precursor ratios. A multiple regression was used to assess the association between fatty acid and C-peptide concentrations. C-peptide concentrations were associated inversely with linoleic acid levels in cholesterol ester and phospholipid (P for trend = .01 and .02, respectively) and positively with stearic and palmitoleic acids in cholesterol ester (P for trend = .02 and .006, respectively) and dihomo-γ-linolenic acid in cholesterol ester and phospholipid (P for trend < .0001 for both). C-peptide concentrations were not associated with n-3 polyunsaturated fatty acids. C-peptide concentrations significantly increased as δ-9-desaturase (16:1 n-7/16:0) and δ-6-desaturase (18:3 n-6/18:2 n-6) increased (P for trend = .01 and .03, respectively) and δ-5-desaturase (20:4 n-6/20:3 n-6) decreased (P for trend = .004). In conclusion, a fatty acid pattern with high levels of serum stearic, palmitoleic, or dihomo-γ-linolenic acids; δ-9-desaturase (16:1 n-7/16:0) or δ-6-desaturase (18:3 n-6/18:2 n-6) activities; and low levels of serum linoleic acid or δ-5-desaturase (20:4 n-6/20:3 n-6) activity might be associated with higher insulin resistance in Japanese adults. © 2012 Elsevier Inc..
49. Yasumi Kimura, Masao Sato, Kayo Kurotani, Akiko Nanri, Kazuaki Kawai, Hiroshi Kasai, Katsumi Imaizumi, Tetsuya Mizoue, PUFAs in serum cholesterol ester and oxidative DNA damage in Japanese men and women, American Journal of Clinical Nutrition, 10.3945/ajcn.111.030817, 95, 1209-1214, 2012.05, Background: PUFAs are susceptible to lipid peroxidation and play a role in inflammation, both of which can induce oxidative stress. However, the relation of PUFA to oxidative DNA damage in humans is elusive.

Objective: We examined the association between concentrations of circulatory PUFAs and urinary 8-oxo-7,8-dihydroguanine (8-oxoGua) in Japanese men and women.

Design: The subjects were 495 participants (290 men and 205 women) in a cross-sectional study in 2 municipal offices in Japan. Serum cholesterol ester (CE) and phospholipid fatty acid composition were measured by gas-liquid chromatography. Urinary 8-oxoGua concentrations were measured by HPLC, and 8-oxoGua values for each tertile of PUFA after adjustment for covariates were calculated by multiple regression.
Results: Urinary 8-oxoGua concentrations increased with increasing concentrations of n–3 (omega-3) PUFAs, EPA, and DHA in serum CE (P-trend = 0.001, 0.01, and 0.009, respectively), whereas they decreased with increasing concentrations of n–6 PUFAs and linoleic acid (P-trend = 0.02 and 0.051, respectively).
Conclusion: Oxidative DNA damage may be greater with higher concentrations of long-chain n–3 PUFAs but lower with higher concentrations of n–6 (omega-6) PUFAs.
50. Li Tao Tong, Yumiko Fujimoto, Naoki Shimizu, Mariko Tsukino, Taiki Akasaka, Yukiko Kato, Wakako Iwamoto, Sawako Shiratake, Katsumi Imaizumi, Masao Sato, Rice α-globulin decreases serum cholesterol concentrations in rats fed a hypercholesterolemic diet and ameliorates atherosclerotic lesions in apolipoprotein E-deficient mice, Food Chemistry, 10.1016/j.foodchem.2011.10.056, 132, 1, 194-200, 2012.05, The hypocholesterolemic and antiatherogenic effects of rice α-globulin remain unclear. We investigated the hypocholesterolemic effect of rice α-globulin in rats fed a hypercholesterolemic diet. The rats were divided into 4 groups and were orally administrated the following three proteins or a vehicle for 4 weeks: rice protein, rice α-globulin, or soy β-conglycinin at a dose of 100 mg/kg body weight or carboxymethylcellulose to the control rats. In the rice α-globulin group, serum cholesterol concentrations were 28% lower than the control group and fecal neutral steroid excretion was increased by 30%. The hypocholesterolemic effect of rice α-globulin was equal to soy β-conglycinin in SD rats fed the hypercholesterolemic diet. However, the serum cholesterol concentrations in the rice protein group did not change compared to the control group. To investigate the antiatherogenic effects of rice α-globulin, male apolipoprotein E-deficient mice were orally administered the same dose of rice α-globulin for 9 weeks. The en face lesion area in the aorta was 46% lower than in the control group. In conclusion, administration of rice α-globulin improves hypercholesterolemia in rats fed a hypercholesterolemic diet by increasing the fecal excretion of neutral sterols, and inhibits atherosclerosis development in apolipoprotein E-deficient mice. The anti-atherosclerotic effect exerts by mechanism(s) other than the regulation of serum MCP-1 and NO concentrations. © 2011 Elsevier Ltd. All rights reserved..
51. Masaki KATO, Yusuke ITO, Yasutake TANAKA, Masao SATO, Katsumi IMAIZUMI, Nao INOUE, and Ikuo IKEDA, SHRSP/Izm and WKY/NCrlCrlj Rats Having Missense Mutation in Abcg5 Deposited Plant Sterols in the Body, But not Change Their Biliary Secretion and Lymphatic Absorption --- Comparison with Jcl:Wistar and WKY/Izm Rats , Bioscience, Biotechnology, and Biochemistry, 2012.03.
52. Tadateru HAMADA, Mariko KUDO, Masao SATO, Nao INOUE, Koji NAGAO, Katsumi IMAIZUMI and Ikuo IKEDA, Effect of a Liver X Receptor Agonist on Deposition and Lymphatic Absorption of Plant Sterols in Stroke-Prone Spontaneously Hypertensive Rats Having a Mutation in ATP-Binding Cassette Transporter G5, Bioscience, Biotechnology, and Biochemistry, 10.1271/bbb.110554, 75, 12, 2359-2363, 2012.03.
53. Kalpana Poudel-Tandukara, Masao Sato, Yuko Ejima, Akiko Nanric, Yumi Matsushitac, Katsumi Imaizumib, Tetsuya Mizoue, Relationship of serum fatty acid composition and desaturase activity to C-reactive protein in Japanese men and women, Atherosclerosis, 0.1016/j.atherosclerosis.2011.11.012 , 220, 2, 520-524, 2012.02, Background: Although fatty acid composition in serum and desaturase activity, which alters serum fatty acid composition, has been associated with C-reactive protein (CRP) concentration in Western populations, no study has been carried out in non-Western populations. We examined the association of serum fatty acids and estimated desaturase activity with CRP concentrations in Japanese men and women. Methods: Subjects were 489 Japanese municipal employees aged 21-67 years who participated in a survey at the time of a periodic health check-up. Serum high-sensitivity CRP concentrations were measured using the latex agglutination nephelometry method. Fatty acid composition was measured in serum cholesteryl esters and desaturase activities by fatty acid product-to-precursor ratios. Relationships were assessed using multiple regression. Results: Serum CRP concentration was positively associated with palmitic acid (P for trend = 0.002) and inversely with alpha-linolenic acid (P for trend = 0.01) in men, and positively with dihomo-gamma-linolenic acid (P for trend in men or women = 0.01) and inversely with delta-5-desaturase (20:4n-6/20:3n-6) (P for trend in men and women = 0.05 and 0.002, respectively) in men and women. Conclusions: Low-grade inflammation may be associated with a serum fatty acid pattern of high palmitic acid or low alpha-linolenic acid in men, and of high dihomo-gamma-linolenic acid or low delta-5-desaturase in both sexes..
54. Bungo Shirouchi, Sayaka Kawamura, Ryosuke Matsuoka, Sanae Baba, Kazuko Nagata, Sawako Shiratake, Hiroko Tomoyori, Katsumi Imaizumi, and Masao Sato, Dietary guar gum reduces lymph flow and diminishes lipid transport in thoracic duct-cannulated rats, Lipids, DOI: 10.1007/s11745-011-3570-0, 46, 8, 789-793, 2011.08.
55. Masao Sato, Takatoshi Ueda , Kazuko Nagata , Sawako Shiratake , Hiroko Tomoyori , Mitsuo Kawakami , Yukio Ozaki , Hiroshi Okubo , Bungo Shirouchi , Katsumi Imaizumi, Dietary kakrol (Momordica dioica Roxb.) flesh inhibits triacylglycerol absorption and lowers the risk for development of fatty liver in rats, Experimental Biology and Medicine, 10.1258/ebm.2011.011037, 236, 1139-1146, 2011.05.
56. Yukio Kadooka, Akihiro Ogawa, Ken Ikuyama, Masao Sato, The probiotic Lactobacillus gasseri SBT2055 inhibits enlargement of visceral adipocytes and upregulation of serum soluble adhesion molecule (sICAM-1) in rats, International Dairy Journal, 10.1016/j.idairyj.2011.02.001, 2011.04.
57. Li-Tao Tong, Yoshinori Katakura, Sayaka Kawamura, Sanae Baba, Yasutake Tanaka, Miyako Udono, Yoshie Kondo, Kumi Nakamura, Katsumi Imaizumi and Masao Sato, Effects of Kurozu concentrated liquid on adipocyte size in rats, Lipids in Health and Disease, 10.1186/1476-511X-9-134, 9, Article NO. 134, 2010.11, [URL].
58. A Nanri, Y Kimura, Y Matsushita, M Ohta, M Sato, N Mishima, S Sasaki, T Mizoue, Dietary patterns and depressive symptoms among Japanese men and women, European Journal of Clinical Nutrition, 64, 8, 832-839, 2010.08.
59. Yukio Kadooka, Masao Sato, Katsumi Imaizumi, Akihiro Ogawa, Ken Ikuyama, Yoshihito Akai, Masako Okano , Masatoyo Kagoshima, Takashi Tsuchida, Regulation of abdominal adiposity by probiotics (Lactobacillus gasseri SBT2055) in adults with obese tendencies in a randomized controlled trial, European Journal of Clinical Nutrition, 64, 6, 636-643, 2010.06.
60. Akiko Nanri, Mizoue, T, Matsushita, T., Saaki, S., Ohta, T., MASAO SATO, Mishima, N., Serum folate and homocysteine and depressive symptoms among Japanese men and women, European Journal of Clinical Nutrition, 10.1038/ejcn.2009.143, 64, 3, 289-296, 2010.03.
61. Akiko Nanri, Tetsuya Mizoue, Yumi Matsushita, Kalpana Poudel-Tandukar, Masao Sato, Masanori Ohta, Norio Mishima, Association between serum 25-hydroxyvitamin D and depressive symptoms in Japanese: analysis by survey season, European Journal of Clinical Nutrition, 63, 12, 1444-1447, 2009.10.
62. Toshiro Matsui, Masao Sato, Mitsuru Tanaka, Yasuna Yamada, Shinpei Watanabe, Yumiko Fujimoto, Katsumi Imaizumi, Kiyoshi Matsumoto, Vasodilating di-peptide Trp-His can prevent atherosclerosis in apolipoprotein E-deficient mice, British J. Nutrition, 103, 3, 309-313, 2009.08.
63. Kalpana Poudel-Tandukara, Akiko Nanri, Yumi Matsushita, Satoshi Sasaki, Masanori Ohta, Masao Sato, Tetsuya Mizouea, Dietary intake of alpha-linolenic acid and linoleic acid is inversely associated with serum C-reactive protein levels among Japanese men, Nutrition Research, 29, 363-370, 2009.07.
64. Masao Sato, Yasuna Yamada, Hiromi Matsuoka, Sawako Nakashima, Tomoyasu Kamiya, Motoya Ikeguti Katsumi Imaizumi, Dietary Pine bark Extract Reduces Atherosclerotic Lesion Development in Male ApoE-Deficient Mice by Lowering the serum Cholesterol Level, Biosci. Biotechnol. Biochem., 73, 1-4, 2009.07.
65. Ikuo Ikeda, Mariko Kudo, Tadateru Hamada, Koji Nagao, Yoko Oshiro, Masaki Kato, Takashi Sugawara, Takashi Yamahira, Hiroko Ito, Shogo Tamaru, Masao Sato, Katsumi Imaizumi, Satoshi Nagaoka and Teruyoshi Yanagita, Dietary soy protein isolate and its undigested high molecular fraction upregulate hepatic ATP-binding cassette transporter G5 and ATP-binding cassette transporter G8 mRNA and increase biliary secretion of cholesterol in rats, Journal of Nutritional Science and Vitaminology, 55,252-256, 2009.04.
66. Makoto Asahina, Waka Haruyama, Yasuhiro Ichida, Mai Sakamoto, Masao Sato, Katsumi Imaizumi , Identification of SMEK2 as a candidate gene for regulation of responsiveness to dietary cholesterol in rats , Journal of Lipid Research, 50:41-46, 2009.01.
67. Ichiko Miyamoto, Jun Shiono, Jie Liu, Kuniyoshi Shimizu, Masao Sato, Akiko Kukita, Toshio Kukita, Ryuichiro Kondo, Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum, European Journal of Pharmacology , 6002(1), 1-7, 2009.01.
68. S. Fatmawatia,, K. Kurashiki, S. Takeno, Y. Kim, kuniyoshi shimizu, MASAO SATO, katsumi imaizumi, K. Takahashi, S. Kamiya, S. Kaneko, RYUICHIRO KONDO, The inhibitory effect on aldose reductase by an extract of Ganoderma lucidum, Phytotherapy Research , 10.1002/ptr.2425, 23, 1, 28-32, 2009.01.
69. Hyun-jung BANG, Chiyo ARAKAWA, Michihiro TAKADA, Masao SATO, and Katsumi IMAIZUMI, A Comparison of the Potential Unfavorable Effects of Oxycholesterol and Oxyphytosterol in Mice: Different Effects, on Cerebral 24S-Hydroxychoelsterol and Serum Triacylglycerols Levels, Biosci. Biotechnol. Biochem., 72, 12, 3128-3133, 2008.12, [URL].
70. E. M. Hamad, M. Sato, K. Uzu, T. Yoshida, H. Kadooka, H. Kawakami, H. Matsuyama, I. A. A. El-Gawaqd, and K. Imaizumi, Milk fermented by Lactobacillus gasseri SBT2055 influences adipocyte size via inhibition of dietary fat absorption in Zucker rats, Br. J. Nutr., 1-9, 2008.08.
71. R.Matsuoka, M. Kimura, A. Muto, Y. Masuda, M. Sato, K. Imaizumi, Mechanism for the Cholesterol-Lowering Action of Egg White Protein in Rats, Biosci. Biotechnol. Biochem., 72(6), 1506-1512, 2008.06.
72. Kentaro Murakami, Tetsuya Mizoue, Satoshi Sasaki, Masanori Ohta, Masao Sato, Yumi Matsushita, and Norio Mishima, Dietary intake of folate, other B vitamins, and n-3 polyunsaturated fatty acids in relation to depressive symptoms in Japanese adults, Nutrition, 24(2), 140-147, 2008.02.
73. Masao Sato, Yayoi Kawata, Kazuo Erami, Ikuo Ikeda, Katsumi Imaizumi, LXR agonist increases the lymph HDL transport in rats by promoting reciprocally intestinal ABCA1 and apo A-I mRNA levels, Lipids, 10.1007/s11745-007-3131-8, 43, 2, 125-131, 2008.02, Liver and intestine are major sites of apo A-I synthesis in mammals. ABCAI is reported to be involved in the secretion of nascent HDL from cultured intestinal cells. However, whether ABCA1 participates in the secretion of nascent HDL from the intestine has not been assessed directly in vivo. This study examined the effect of a synthetic LXR-agonist "TO" on the lymphatic transport of HDL in thoracic duct-cannulated rats. The feeding of a TO-containing diet resulted in an increased transport of cholesterol and apo A-I in the lymph d > 1.063 g/ml lipoprotein fraction than did the feeding of a control diet without TO. The transport of cholesterol in whole lymph was lower, whereas the transport of apo A-I was higher, in the TO group. The abundance of mRNAs for ABCAI and apo A-I in the intestine was increased in the TO group. Furthermore, although the TO-containing diet reportedly increased the serum HDL concentration in intact mice and rats, no such effect was observed in the cannulated rats. The LXR agonist stimulated in vivo the synthesis of nascent HDL by increasing reciprocally the mRNA for ABCAI and apo A-I in the intestine, thereby contributing to an increase in the circulating HDL. © 2007 AOCS..
74. M. Sato, K. Uzu, T. Yoshida, E. M. Hamad, H. Kawakami, H. Matsuyama, I. A. A. El-Gawaqd, and K. Imaizumi, Effects of milk fermented by Lactobacillus gasseri SBT2055 on adipocyte size in rats,, Br. J. Nutr., 99, 1013-1017, 2007.12.
75. Masao Sato, Hiroyoshi Sato, Akiko Ogawa, Run Nomura, Shinichiro Takashima, Hyun Jung Bang, Hiromi Matsuoka, Katsumi Imaizumi, Antiatherogenic effect of isoflavones in ovariectomized apolipoprotein E-deficient mice, Journal of Agricultural and Food Chemistry, 10.1021/jf071771q, 55, 22, 8967-8971, 2007.10, The consumption of isoflavone-containing foods such as soybean and soybean products has been reported to have beneficial effects on the cardiovascular system in postmenopausal women. The present study was carried out to examine the mechanism underlying the beneficial effects of isoflavones in apolipoprotein (apo) E-deficient mice subjected to ovarian resection. Compared with sham-operated mice, ovariectomized mice had a larger arterial lesion area in the aortic root. Feeding the ovariectomized mice an isoflavone-containing diet (0.055 mg/kJ of total isoflavones/cal of diet) reduced the size of these lesions more than did feeding them with an isoflavone-free diet. Neither ovariectomy nor diet had a significant effect on the concentration of cholesterol in serum and urinary levels of isoprostanes, which are biomarkers for oxidative stress in vivo. The ovariectomized mice showed a greater increase in mRNA abundance for monocyte chemoattractant protein (MCP)-I in the aorta and in the level of nitric oxide (NO) secreted by peritoneal macrophages in culture than did the sham-operated mice. The isoflavone-containing diet lowered the MCP-I expression and the NO secretion more than did the isoflavone-free diet. These results suggest that dietary isoflavones confer an antiatherogenic effect by preventing the activation of macrophages due to the removal of ovaries. © 2007 American Chemical Society..
76. M. Sato, H. Sato, A. Ogawa, R. Nomura, S. Takashima, H.-J. Bang, H. Matsuoka and K. Imaizumi, Antiatherogenic effect of isoflavones in ovariectomized apolipoprotein E-deficient mice, J. Agr. Food Chem., 55 (22), 8967–8971, 2007.09.
77. Effect of Dietary Protein on Fatty Acid Desaturase Expression and Action in Rat Liver.
78. Change in Activity of Soybean Lipoxidases, [URL].
79. M.Sato, S.Kenichi, R.Nomura, D.Kawamoto, R.Nagamine and K.Imaizumi, Linoleic acid-rich fats reduce atherosclerosis development beyond its oxidative and inflammatory stress-increasing effect in apolipoprotein E-deficient mice in comparison with saturated fatty acid-rich fats., Br. J. Nutr., 10.1079/BJN20051409, 94, 6, 896-901, 94,896-901, 2005.12.
80. M. Asahina, M. Sato , K. Imaizumi, Genetic analysis of diet-induced hypercholesterolemia in exogenously hypercholesterolemic rats, Journal of Lipid Research, 10.1194/jlr.M5000257-JLR200, 46, 10, 2289-2294, 46: 2289-2294, 2005.10.
81. 日本人におけるコレステロール酸化物と植物ステロール酸化物の摂取量.
82. Hiroyoshi Sato, Satoko Nishida, Hiroko Tomoyori, Masao Sato, Ikuo Ikeda, Katsumi Imaizumi, Oxysterol regulation of estrogen receptor α-mediated gene expression in a transcriptional activation assay system using HeLa cells, Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.68.1790, 68, 8, 1790-1793, 2004.08, In order to test the estrogenic activity of sterol oxidation products from cholesterol and phytosterols, an estrogen-dependent gene expression assay was performed in estrogen receptor α-stably transformed HeLa cells. The ranking of the estrogenic potency of these compounds was different: 17β-estradiol ≫ genistein ≫ β-epoxycholesterol = daidzein = cholestanetriol = 22(R)-hydroxycholesterol = 20(S)-hydroxycholesterol = sitostanetriol > campestanetriol = β-epoxysitosterol = 7β-hydroxycholesterol. These compounds were not estrogenic in estrogen receptor-negative HeLa cells..
83. Hiroko Tomoyori, Yayoi Kawata, Tomoko Higuchi, Ikuyo Ichi, Hiroyoshi Sato, Masao Sato, Ikuo Ikeda, Katsumi Imaizumi, Phytosterol oxidation products are absorbed in the intestinal lymphatics in rats but do not accelerate atherosclerosis in apolipoprotein E-deficient mice, Journal of Nutrition, 10.1093/jn/134.7.1690, 134, 7, 1690-1696, 2004.07, Phytosterol oxidation products (oxyphytosterols) are formed during the processing and storage of foods. However, it is unknown whether oxyphytosterols affect human health. To address these issues, we prepared β-sitosterol and campesterol oxides, evaluated their lymphatic absorption in rats, and examined the effect of an oxyphytosterol diet on atherosclerosis in apolipoprotein (apo) E-deficient mice. The lymphatic absorption of cholesterol and 6 oxyphytosterols (7α-hydroxy, 7β-hydroxy, β-epoxy, α-epoxy, dihydroxy, and 7-keto) of β-sitosterol or campesterol was assessed in thoracic duct-cannulated rats fed an AIN-93G-based diet containing 2.5 g of cholesterol, oxyphytosterols, or intact phytosterols per kg. Lymphatic recoveries (on a mass basis) of oxycampesterols (15.9 ± 2.8%, n = 10) and oxysitosterols (9.12 ± 1.77%, n = 10) were higher than for campesterol (5.47 ± 1.02%, n = 12, P < 0.05) and β-sitosterol (2.16 ± 0.37%, n = 12, P < 0.05), but lower than for cholesterol (37.3 ± 8.3%, n = 6, P < 0.05). Apo E-deficient mice were fed an AIN-93G-based diet containing 0.2 g oxyphytosterols or intact phytosterols per kg for 9 wk. Diet-derived oxyphytosterols accumulated in the serum, liver, and aorta. Furthermore, the oxyphytosterol diet increased oxycholesterol in the serum compared to the phytosterol diet. However, there was no significant difference between the 2 groups in the serum and aortic cholesterol concentration, the lesion area in the aortic root, or 8-iso-prostaglandin F2α concentration in the urine. These results indicate that exogenous oxyphytosterols are well-absorbed and accumulate in the body, but do not promote the development of atherosclerosis in apo E-deficient mice..
84. Weihua Ni, Yasuyuki Tsuda, Shinichiro Takashima, Hiroyoshi Sato, Masao Sato, Katsumi Imaizumi, Anti-atherogenic effect of soya and rice-protein isolate, compared with casein, in apolipoprotein E-deficient mice, British Journal of Nutrition, 10.1079/BJN2003878, 90, 1, 13-20, 2003.07, Our objective was to determine whether dietary plant proteins such as soya-protein isolate (SPI) and rice-protein isolate (RPI) compared with animal proteins, such as casein, could afford beneficial effects on atherosclerosis development in apolipoprotein E-deficient mice. In experiment 1, male and female mice were fed on a purified diet containing either casein, SPI or RPI for 9 weeks. The en face lesion area in the aorta (P<0.05) and the lesion size in the aortic root (P<0.05) in mice fed the casein-based diet were greater than those in the SPI or RPI groups. The plant protein groups had an increased concentration of serum L-arginine (P<0.05) and NO metabolites (NO2 plus NO3) (P<0.05) than did the casein group. The inhibitory effect of the plant proteins on the lesion formations was unrelated to gender and total serum cholesterol. In experiment 2, the L-arginine and L-methionine contents were the same in the L-arginine-supplemented casein-based and SPI-based diets, and between the L-methionine-supplemented SPI-based and the casein-based diets. Male mice were fed on the diets for 15 weeks. There were no significant differences in the en face lesion area and the lesion size between the casein group and the L-arginine-supplemented group, although the serum L-arginine (P<0.05) and NO2 plus NO3 (P<0-05) concentrations in the supplemented group were higher than those in the casein group. There were no significant effects of L-methionine supplementation on the lesion formations. In experiment 3, male mice were given the casein-based diet or the L-arginine-supplemented casein-based diet together with water or water containing an NO synthesis inhibitor for 9 weeks. When given the casein-based diet, the inhibitor drinking, compared with water drinking, resulted in a reduction of the serum NO2 plus NO3 concentration (P<0.01) and an increase in the en face lesion area (P<0.05) and the lesion size (P<0.01). When given the L-arginine-supplemented diet, the inhibitor drinking, compared with water drinking, resulted in no increase in the lesion area and size. These results demonstrate anti-atherogenic potentials of SPI- as well as RPI-derived proteins, but their L-arginine and L-methionine contents were not sufficient enough to explain the underlying mechanism(s)..
85. W.H.Ni, Y.Tsuda, S.Takashima, H.Sato, M.Sato and K.Imaizumi, Antiatherogenic effect of soy and rice protein isolate, compared with casein, in apolipoprotein E-deficient mice., Br. J. Nutr., 90: 13-20, 2003.01.
86. Yoko Shoji, Ayako Sanekata, Masao Sato, Katsumi Imaizumi, Preparation of antiserum against rat Δ6-desaturase and its use to evaluate the desaturase protein levels in rats treated with gemfibrozil, a ligand for peroxisome proliferator-…, Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.67.1177, 67, 5, 1177-1178, 2003.01, Anti-rat Δ6-desaturase serum was produced by immunizing rabbits with the 14 N-terminal amino acids (2-15) of rat Δ6-desaturase. The antiserum prevented the enzymatic activity of Δ6-desaturase in microsomes. Subsequently, the antiserum was used to demonstrate that gemfibrozil, a ligand for peroxisome proliferator-activated receptor α, is involved in activating Δ6-desaturase gene expression, thereby elevating the protein level and the activity. © 1999 by Japan Society for Bioscience, Biotechnology, and Agrochemistry..
87. Ikuo Ikeda, Ryosuke Matsuoka, Tadateru Hamada, Kosuke Mitsui, Sachiko Imabayashi, Akira Uchino, Masao Sato, Eiichi Kuwano, Tomoaki Itamura, Koji Yamada, Kazunari Tanaka, Katsumi Imaizumi, Cholesterol esterase accelerates intestinal cholesterol absorption, Biochimica et Biophysica Acta - General Subjects, 10.1016/S0304-4165(02)00204-0, 1571, 1, 34-44, 2002.05, Mechanisms of acceleration of cholesterol absorption by cholesterol esterase were investigated in various experimental conditions. Lymphatic recovery of cholesterol intubated as a micellar solution containing phosphatidylcholine (PC) into the duodenum was enhanced by the co-administration of cholesterol esterase in rats drained of bile and pancreatic juice. However, no accelerated incorporation was observed when cholesterol was solubilized in PC-depleted micelles. Cholesterol esterase dose-dependently accelerated the incorporation of cholesterol into differentiated Caco-2 cells, only when cholesterol was solubilized in PC-containing micelles. The accelerated incorporation of cholesterol into Caco-2 cells by cholesterol esterase disappeared when the enzyme was preincubated with a suicide inhibitor of cholesterol esterase. Cholesterol esterase has an activity as phospholipase A2. When 10% of PC in bile salt micelles was replaced by lysophosphatidylcholine (lysoPC), the incorporation of cholesterol into Caco-2 cells was significantly accelerated. Cholesterol esterase enhanced the incorporation of micellar cholesterol into brush border membranes prepared from the rat jejunum. The addition of cholesterol esterase to bile salt micelles accelerated the release of micellar cholesterol in a dose-dependent manner, only when the micelles contained PC. These observations strongly suggest that cholesterol esterase hydrolyzes PC in bile salt micelles and thereby, accelerating the release of cholesterol from bile salt micelles. This may be a major cause of the acceleration of cholesterol absorption by cholesterol esterase. © 2002 Elsevier Science B.V. All rights reserved..
88. M.Iwamoto, M.Kono, D.Kawamoto, H.Tomoyori, M.Sato and K.Imaizumi, Differential effect of walnut oil and safflower oil on the serum cholesterol level and lesion area in the aortic root of apolipoprotein E-deficient mice., Biosci. Biotechnol. Biochem., 66, 1, 141-146, 66:141-146, 2002.01.
89. H.Tomoyori, O.Carvajal. M.Nakayama, T.Kishi, M.Sato, I.Ikeda and K.Imaizumi, Lymphatic transport of dietary cholesterol oxidation products, cholesterol and triacylglycerols in rats., Biosci. Biotechnol. Biochem., 66, 4, 828-834, 66 : 828-834, 2002.01.
90. M.Iwamoto, K.Imaizumi, M.Sato, Y.Hirooka, K.Sakai, A.Takeshita and M.Kono, Serum lipid profiles in Japanese women and men during consumption of walnuts., Eur. J. Clin. Nutr., 10.1038/sj.ejcn.1601400, 56, 7, 629-637, 56:629-637, 2002.01.
91. Mioko Kusaba-Nakayama, Masami Ki, Emiko Kawada, Masao Sato, Ikuo Ikeda, Toshihiro Mochizuki, Katsumi Imaizumi, Intestinal absorbability of wheat allergens, subunits of a wheat α-amylase inhibitor, expressed by bacteria, Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.65.2448, 65, 11, 2448-2455, 2001.11, Wheat CM2, CM3 and CM16 proteins are known as subunits of the tetrameric α-amylase inhibitor as well as major allergens to baker's asthma. The purpose of this study is to produce these CM proteins by bacteria in a quantity adequate for studying the penetration characteristics of the CM proteins through intestinal mucosa in rats and Caco-2 cells. cDNAs encoding the mature proteins were expressed in Escherichia coli and purified by an Ni2 + -chelating column. The recombinant proteins were radioiodinated and admministered orally to rats or applied to the apical site of the Caco-2 cell monolayer. The radioactivity in the trichloroacetic acid-insoluble fraction, which was mainly composed of peptides with molecular mass less than that of the intact CM proteins, in the serum and the basolateral medium was highest in recombinant CM3. Accordingly, the intestinal absorption of these three proteins in the form present in wheat should be evaluated..
92. Mei Chu Hung, Kaoru Hayase, Riki Yoshida, Masao Sato, Katsumi Imaizumi, Cerebral protein kinase C and its mRNA level in apolipoprotein E-deficient mice, Life Sciences, 10.1016/S0024-3205(01)01221-8, 69, 12, 1419-1427, 2001.08, It is known that protein kinase C (PKC) activity may be one of the fundamental cellular changes associated with memory function. Apolipoprotein E (apoE) deficiency causes cholinergic deficits and memory impairment. ApoE-deficient mouse has been employed as a serviceable model for studying the relation between apoE and the memory deficit induced by cholinergic impairment. Brain-fatty acid binding protein (b-FABP) might be functional during development of the nervous system. Peroxisome proliferator-activated receptor (PPAR) is involved in the early change in lipid metabolism. We investigated the alterations not only in cerebral PKC activity, but also in the gene expressions of PKC-β, brain-FABP and PPAR-α in apoE-deficient mice. The results showed that there was a lower cerebral membrane-bound PKC activity in the apoE-deficient mice than in its wild type strain (C57BL/6). But there were no significant differences in cytosolic PKC activity. PKC-β, b-FABP and PPAR-α mRNA expressions in cerebrum were lowered in apoE-deficient mice. These findings may be involved in the dysfunction of the brain neurotransmission system in apoE-deficient mouse. Alternatively, these results also suggest that cerebral apoE plays an important role in brain PKC activation by maintaining an appropriate expression of b-FABP and PPAR-α mRNAs. © 2001 Elsevier Science Inc. All rights reserved..
93. K.Nagao, M.Sato, M.Takenaka, M.Ando, M.Iwamoto and K.Imaizumi, Feeding unsaponifiable compounds from rice bran oil dose not alter hepatic mRNA abundance for cholesterol metabolism-related proteins in hypercholesterolemic rats., Biosci. Biotechnol. Biochem., 10.1271/bbb.65.371, 65, 2, 371-377, 65:371-377, 2001.01.
94. M.C.Hung, K.Shibasaki, R.Yoshida, M.Sato and K.Imaizumi, Learning behavior and cerebral protein kinase C, antioxidant status, lipid composition in senescence- accelerated mouse: influence of a phosphatidylcholine-vitamin B12 diet., Br. J. Nutr., 10.1079/BJN2001391, 86, 2, 163-171, 86:163-171, 2001.01.
95. M.Sato, S.Yoshida, K.Nagao, S.Nishizono, M.Kusaba, M.C.Hung, I.Ikeda and K.Imaizumi, Application of the random arbitrary primed polymerase chain reaction differential display method to isolate genes of cholesterol metabolism-related proteins from rat liver., Biosci. Biotechnol. Biochem., 10.1271/bbb.64.1058, 64, 5, 1058-1060, 64:1058-1060, 2000.01.
96. M.C.Hung, K.Shibasaki, S.Nishizono, M.Sato, I.Ikdeda, Y.Masuda, M.Kunou, M.Kawamura, M.Yamashita, S.Inoue, and K.Imaizumi, Ibotenic acid-induced lesions of the medial septum increase hippocampal membrane asociaed protein kinase C activity and reduce acetylcholine synthesis: Prevention by a phosphatidylcholine/vitamin B12 diet., J. Nutr. Biochem., 10.1016/S0955-2863(99)00088-1, 11, 3, 159-164, 11:159-164, 2000.01.
97. O.Carvajal, M.Nakayama, T.Kishi, M.Sato, I.Ikeda, M.Sugano and K.Imaizumi, Positional distributiion of medium chain fatty acid in dietary synthetic triacylglycerol on lymphatic lipid transport and chemical composition of chylomicrons in rats with perment cannulation of thoracic duct., Lipids, 35, 1345-1351, 2000.01.
98. M.Sato, S.Yoshida, K.Nagao and K.Imaizumi, Superiority of dietary safflower oil over olive oil in lowering serum cholesterol and increasing hepatic mRNAs for the LDL receptor and cholesterol 7-hydroxylase in exogenously hypercholesterolemic (ExHC) rats., Biosci. Biotechnol. Biochem., 10.1271/bbb.64.1111, 64, 6, 1111-1117, 64:1111-1117, 2000.01.
99. Octavio Carvajal, Masanobu Sakono, Hirofumi Sonori, Masahiro Nakayama, Taiji Kishi, Masao Sato, Ikuo Ikeda, Michihiro Sugano, Katsumi Imaizumi, Structured triacylglycerol containing medium-chain fatty acids in sn-1(3) facilitates the absorption of dietary long-chain fatty acids in rats, Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.64.793, 64, 4, 793-798, 2000.01, A study was carried out to examine if the positional distribution of medium chain fatty acids (MCF) in triacylglycerol influences dietary fat absorption in rats. Two types of structure-specific fats, one predominantly composed of MCF in sn-1(3) and linoleic acid in sn-2 [sn-1(3)MCF-structured] and the others of MCF in sn-2 and linoleic acid in sn-1(3) [sn-2MCF-structured], were initially prepared, and the two structure-specific fats were interesterified and designated as sn-1(3)MCF-interesterified and sn-2MCF-interesterified. Synthetic fat was mixed with an equal amount of cocoa butter (103 g/kg of diet) and was supplemented to the AIN93G-based diet. Rats were fed on the diets for 4 wk. Long-chain saturated fatty acids were the predominant fatty acids excreted into the feces, and the positional distribution of MCF resulted in an altered fat absorption rate (%) of 81.8, 82.5, 84.2 and 86.3 for the rats fed on the diets containing sn-2MCF-structured, sn-1(3)MCF-interesterified, sn-2MCF-interesterified and sn-1(3)MCF-structured fats, respectively. The proportion of MCF in the serum, liver and adipose tissue triacylglycerols was not affected by the MCF distribution of the dietary fats. These results indicate that the distribution of MCF in dietary triacylglycerol is a determinant of intestinal fat absorption. © 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry..
100. Y.Adan, K.Shibata, W.H.Ni, Y.Tsuda, M.Sato, I.Ikeda and K.Imaizumi, Concentration of serum lipids and aortic lesion size in female and male apo E-deficient mice fed docosahexaenoic acid., Biosci. Biotech. Biochem., 10.1271/bbb.63.309, 63, 2, 309-313, 63:309-313, 1999.01.
101. Y.Adan, K.Shibata, M.Sato, I.Ikeda and K.Imaizumi, Effects of docosahecaenoic and eicosapentaenoic acid on lipid metabolism, eicosanoid production, platelet aggregation and atherosclerosis in hyercholesterolemic rats., Biosci. Biotech. Biochem., 10.1271/bbb.63.111, 63, 1, 111-119, 63: 111-119, 1999.01.
102. W.H.Ni, S.Yoshida, Y.Tsuda, K.Nagao, M.Sato and K.Imaizumi, Ethanol-extracted soy protein isolate results in elevation of serum cholesterol in exogenously hypercholesgterolemic rats., LIPIDS, 10.1007/s11745-999-0417-1, 34, 7, 713-716, 34:713-716, 1999.01.
103. K.Nagao, S.Yoshida, H.Nakagiri, M.Sakono, M.Sato and K.Imaizumi, Gemfibrozil reduces non-high-density lipoprotein cholesterol in exogenously hyper- cholesterolemic (ExHC) rats fed a high-cholesterol diet., Comp. Biochem. Physiol., 10.1016/S0305-0491(98)10049-4, 120, 3, 579-586, 120B: 579-586, 1998.01.
104. K Nagao, M Sakono, H Sakurai, M Sato, K Imaizumi, Exogenously hypercholesterolemic rats, compared with their progenitor Sprague-Dawley rats, have altered mRNAs for cholesterol 7 alpha-hydroxylase and low-density-lipoprotein receptor and activities of cholesterol 7 alpha-hydroxylase and acyl-CoA: Cholesterol acyltransferase in the liver in response to dietary cholesterol, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 118, 4, 949-955, 1997.12, Exogenously hypercholesterolemic (ExHC) rats promptly increase serum cholesterol concentration in response to dietary cholesterol. To examine underlying mechanism(s) for this susceptibility, responses of mRNAs for cholesterol metabolism-related proteins and their activities in the liver to dietary cholesterol were compared between ExHC rats and their progenitor Sprague Dawley rats. ExHC rats slightly decreased the abundance of low density lipoprotein (LDL) receptor mRNA in response to dietary cholesterol, although the amount of LDL receptor was not influenced. The abundance of cholesterol 7 alpha-hydroxylase mRNA and the enzyme activity in response to dietary cholesterol were greater in ExHC rats, but the fecal excretion of bile acid was comparable between the strain. Dietary cholesterol-dependent elevation of acyl-CoA:cholesterol acyltransferase activity was greater in ExHC rats. The concentration of liver triacylglycerols was markedly lower in ExHC rats. These results suggest that ExHC rats may increase serum cholesterol by increasing hepatic secretion of cholesteryl ester-rich particles. (C) 1997 Elsevier Science Inc..
105. M.Sakono, T.Fukuyama, W.H.Ni, K.Nagao, H.-R.Ju, M.Sato, K.Sakata, H.Iwamoto and K.Imaizumi, Comparison between dietary soybean protein and casein of the inhibiting effect on atherogenesis in the thoracic aorta of hypercholesterolemic (ExHC) rats treated with experimental hypervitamin D., Biosci. Biotech. Biochem., 61, 3, 514-519, 61:514-519, 1997.01.
106. M.Sakono, H.Mori, K.Nagao, M.Sato, I.Ikeda, K.Yamamoto and K.Imaizumi, ExHC (exogenous hypercholesterlomeic) rats, compared with their progenitor, Sprague-Dawley rats, promptly alter cholesterol metabolism in the liver and secrete cholesterol-rich particles in response to dietary cholesterol., Comp. Biochem. Physiol., 113B: 803-808, 1996.01.
107. M.Sato, K.Nagao, M.Sakono, H.Ogawa, K.Yamamoto and K.Imaizumi, Low protein diets posttranscriptionally repress apolipopoprotein B expression in rat liver., J. Nutr. Biochem., 10.1016/S0955-2863(96)00059-9, 7, 7, 381-385, 7: 381-385, 1996.01.
108. M.Sato, K.Nagao, T.Hayami, M.Sugano and K.Imaizumi, Repression of fat-dependent intestinal apo A-IV mRNA abundance by medium chain triacylglycerols and proteins, and elevation of fat-dependent apo A-IV transport by carbohydrates in suckling rat pups., J. Nutr. Sci. Vitaminol., 41, 3, 293-306, 41:293-306, 1995.01.
109. K.Imaizumi, A.Tominaga, M.Sato and M.Sugano, Effects of dietary sphingolipids on level of serum and liver lipids in rats., Nutr. Res., 10.1016/S0271-5317(05)80024-7, 12, 4-5, 543-548, 12: 543-548, 1992.01.
110. K.Imaizumi, Y.Nagatsu, M.Sato, S.Yasni and M.Sugano, Effect of xylo- oligosaccharides on blood glucose, serum and liver lipids and cecum short-chain fatty acids in diabetic rats., Agric. Biol. Chem., 55: 199-205, 1991.01.