Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Kazuhiro Iiyama Last modified date:2020.06.02

Associate Professor / Agricultural Bioresource Sciences / Department of Bioresource Sciences / Faculty of Agriculture

1. Mai Morishita, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Maximiano Corrêa Cassal, Chisa Yasunaga-Aoki and Kazuhiro Iiyama, Toxin complex is a major virulence determinant of Enterobacter sp. 532 against the silkworm, Bombyx mori, J. Insect Biotechnol. Sericol. , 88, 1, 21-25, 2019.02.
2. Mai Morishita, Akitsu Masuda, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Kosuke Tashiro, Chisa Yasunaga-Aoki, Kazuhiro Iiyama, Identification of insecticidal toxin produced by Enterobacter sp. strain 532 isolated from diseased Bombyx mori silkworms, FEMS Microbiology Letters, 366, 2, fny295, 2019.01, Although Enterobacter sp. 532 shows pathogenicity in Bombyx mori, the insecticidal mechanisms are unclear. Here, we identified and characterised an insecticidal protein from Enterobacter. The insecticidal protein was purified from the strain and inoculated into B. mori larvae. Intracellular proteins were prepared, purified and separated by preparative native polyacrylamide gel electrophoresis (PAGE); one protein band had insecticidal activity. Sodium dodecyl sulfate-PAGE showed the presence of several bands, indicating that the insecticidal protein formed a complex. Peptide mass fingerprinting of a prominent 255.3-kDa band revealed 64 peptides that matched one protein with 33.0% sequence coverage. This protein was a homologue of the A component of the toxin complex (Tc), and the VRP1 domain was conserved; thus, the gene was named itcA (insecticidal toxin complex A). In the itcA downstream region, B and C component gene homologues were found, and these genes were located on an 86.2-kb contig sequence. Two repA genes and 27 genes related to conjugation transfer of plasmids were located on the contig, suggesting that the contig originated from a mobilisable plasmid. Therefore, these findings suggested that the strain may have acquired the Tc genes by horizontal transfer. This is the first description of Tc produced by the genus Enterobacter..
3. Morifuji Y, Xu J, Karasaki N, Iiyama K, Morokuma D, Hino M, Masuda A, Yano T, Mon H, Kusakabe T, Lee JM., Expression, Purification, and Characterization of Recombinant Human α1-Antitrypsin Produced Using Silkworm-Baculovirus Expression System., Molecular Biotechnology, 60, 12, 924-934, 2018.06.
4. Kenji Kikuhara, Kazuhiro Iiyama, Masaru Matsumoto, Naruto Furuya, First report on DMI fungicide resistance in Gymnosporangium asiaticum, the causal agent of Japanese pear rust, in Japan, JOURNAL OF GENERAL PLANT PATHOLOGY, 10.1007/s10327-018-0820-5, 85, 1, 49-56, 2019.01, Japanese pear rust, caused by Gymnosporangium asiaticum Miyabe ex Yamada, is one of the most important fungal plant diseases affecting Japanese pear cultivation. Although sterol demethylation inhibitor (DMI) fungicides have controlled this disease effectively, outbreaks have been frequent in some areas of Japan since 2008. We, therefore, evaluated fungal populations from Fukuoka Prefecture for DMI fungicide sensitivity in vivo. Aeciospores on diseased pear leaves were collected in 2013 from four orchards in Fukuoka Prefecture and one control orchard in Ibaraki Prefecture. Juniperus chinensis var. kaizuka Hort. test plants were sprayed with difenoconazole and fenarimol at recommended rates, and the plants were inoculated with aeciospores. Difenoconazole and fenarimol effectively controlled the population of rust fungus from Ibaraki Prefecture. In contrast, fenarimol and difenoconazole were found to be ineffective against fungal populations from Fukuoka Prefecture. Subsequently, telia were collected from diseased juniper twigs in five areas in Fukuoka Prefecture and one control area in Ibaraki Prefecture in 2013 and 2014. Similarly, test plants of Japanese pear ‘Kousui’ were treated with difenoconazole or fenarimol and inoculated with basidiospores taken from those telia. Both difenoconazole and fenarimol were largely ineffective against the populations from Fukuoka Prefecture. In addition, field trials conducted in an experimental orchard in 2014 and 2015 showed that difenoconazole and fenarimol were not effective in controlling this disease. These results indicate the occurrence of Japanese pear rust resistant to DMI fungicides in Fukuoka Prefecture..
5. Shahadat Hossain, Abu Ashraf Khan, Md Rahman, Kazuhiro Iiyama, Naruto Furuya, First Report of Soft Rot Disease of Papaya Caused by Klebsiella variicola in Bangladesh, J. Fac. Agr., Kyushu Univ., 63, 2, 201-205, 2018.12.
6. Oumi Nishi, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu, Species associations and distributions of soil entomopathogenic fungi Metarhizium spp. in Japan. , Mycology, 8, 4, 308-317, 2017.10.
7. Ming-Ming Ji, Jae Man Lee, Hiroaki Mon, Kazuhiro Iiyama, Tsuneyuki Tatsuke, Daisuke Morokuma, Masato Hino, Mami Yamashita, Kazuma Hirata, Takahiro Kusakabe, Lipidation of BmAtg8 is required for autophagic degradation of p62 bodies containing ubiquitinated proteins in the silkworm, Bombyx mori, INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 10.1016/j.ibmb.2017.08.006, 89, 86-96, 2017.10.
8. Jing Zhang, Saki Taniguchi, Hironori Ito, Kazuhiro Iiyama, Madoka Hino, Tsutomu Katayama, Makoto Kimura, Expression of a Vibrio parahaemolyticus toxin in Escherichia coli results in chromosomal DNA degradation, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 10.1080/09168451.2017.1347486, 81, 10, 1937-1940.
9. Shun-ichiro Takanoa, Midori Tudab, Keiji Takasua, Naruto Furuya, Yuya Imamura, Sangwan Kim, Kosuke Tashiro, Kazuhiro Iiyama, Matias Tavares, Acacio Cardoso Amaral, Unique clade of alphaproteobacterial endosymbionts induces complete cytoplasmic incompatibility in the coconut beetle, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 10.1073/pnas.1618094114, 114, 23, 6110-6115, 2017.06.
10. Mami Yamashita, Jian Xu, Daisuke Morokuma, Kazuma Hirata, Masato Hino, Hiroaki Mon, Masateru Takahashi, Samir M. Hamdan, Kosuke Sakashita, Kazuhiro Iiyama, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee, Characterization of Recombinant Thermococcus kodakaraensis (KOD) DNA Polymerases Produced Using Silkworm-Baculovirus Expression Vector System, 10.1007/s12033-017-0008-9, 59, 6, 221-233, 2017.06.
11. Htet Wai Wai Kyaw, Kenichi Tsuchiya, Masaru Matsumoto, Kazuhiro Iiyama, Seint San Aye, Myo Zaw, Daisuke Kurose, Mitsuo Horita, Naruto Furuya, Genetic diversity of Ralstonia solanacearum strains causing bacterial wilt of solanaceous crops in Myanmar, J Gen Plant Pathol, 59, 221-233, 2017.07, In 2013 and 2014, an extensive survey of bacterial wilt in Myanmar was performed, and 70 strains of Ralstonia solanacearum (Rs) were collected from wilting plants of tomato, potato, chili and eggplant. Myanmar Rs strains were characterized by traditional and molecular methods. Polymerase chain reaction (PCR) test using Rs-specific primer set amplified one specific band (281-bp) from template DNA of all strains. Pathogenicity tests on the four solanaceous plants differentiated the strains into six pathogenic groups. Biovar determination tests showed that biovar 3 strains predominated (63%) among all Rs strains. Biovar 4 strains (7%) were obtained from both tomato and chili strains, whereas biovar 2 (30%) strains were isolated only from potato. Multiplex-PCR analysis indicated that tomato, eggplant and chili strains belonged to phylotype I, whereas potato strains comprised phylotype I and phylotype II. Strains in phylotype I, which was suggested to have originated from Asia, were the most prevalent in all surveyed areas. Phylogenetic analysis based on the endoglucanase (egl) gene sequences revealed that Myanmar strains partitioned into two major clusters that corresponded to phylotype I and II. Strains in phylotype I were further divided into seven subclusters, each corresponding to a distinct sequevar (15, 17, 46, 47, 48, unknown 1 or unknown 2). All strains in phylotype II belonged to sequevar 1. This is the first comprehensive report of the presence of diverse Rs strains in Myanmar..
12. Kazuhiro Iiyama, Mai Morishita, Jae Man Lee, Hiroaki Mon, Takahiro Kusakabe, Kosuke Tashiro, Taiki Akasaka, Chisa Yasunaga-Aoki and Kazuhisa Miyamoto, A reconsideration of the taxonomic position of two bacterial strains isolated from flacherie-diseased silkworms in 1965, Journal of Insect Biotechnology and Sericology, 86, 35-41, 2017.04, Recent advances in bacterial characterization methodologies have made taxonomic categorization significant- ly more accurate. Here, we re-evaluated the position of bacterial strains (532 and 652) belonging to the genus Hafnia, isolated from flacherie-diseased silkworms in 1965. Phylogenetic analysis based on the 16S rRNA gene sequences of these strains suggests that they belong to genus Enterobacter. Using multilocus sequence analy- sis (MLSA), these strains were further classified to MLSA group A, which is a “core” group of Enterobacter con- taining E. cloacae (the type species of the genus). Although these strains were closely related to E. mori, E. tabaci, and E. asburiae, they also had other MLSA characteristics that distinguished them from these neighbor- ing bacterial species. These data were supported by further biochemical analysis. Thus, it appears that the 532 and 652 strains isolated almost half a century ago belong to genus Enterobacter, and their unique characteristics strongly suggest that they are a novel bacterial species..
13. Kazuhiro Iiyama, Eigo Takahashi, Jae Man Lee, Hiroaki Mon, Mai Morishita, Takahiro Kusakabe, Chisa Yasunaga-Aoki, Alkaline protease contributes to pyocyanin production in Pseudomonas aeruginosa., FEMS Microbiology Letters,, 364, fnx051, 2017.04.
14. Kazuhiro Iiyama, Jae Man Lee, Tuneyuki Tatsuke, Hiroaki Mon, Takahiro Kusakabe, Expression and characterization of recombinant Serratia liquefaciens nucleases produced with Baculovirus-mediated silkworm expression system., Molecular Biotechnology, 10.1007/s12033-016-9937-y, 58, 393-403, 2016.06, Baculovirus-Bombyx mori protein expression system has mainly been used for translation of eukaryotic proteins. In contrast, information pertaining to bacterial protein expression using this system is not sufficient. Therefore, recombinant nucleases from Serratia liquefaciens (rSlNucAs) were expressed in a Baculovirus-B. mori protein expression system. rSlNucAs containing the native signal peptide (rSlNucA-NSP) or silkworm 30-K signal peptide (rSlNucA-30K) at the NH2-terminus were constructed to enable secretion into the extracellular fraction. Both rSlNucA-30K and rSlNucA-NSP were successfully secreted into hemolymph of B. mori larvae. Affinity-purified rSlNucAs showed high nuclease activity. Optimum pH was 7.5 and half of maximum activity was maintained between pH 7.0 and 9.5. Optimum temperature was 35 °C. rSlNucAs showed sufficient activity in twofold-diluted radioimmunoprecipitation assay buffer and undiluted, mild lysis buffer. Genomic DNA of Escherichia coli was efficiently digested by rSlNucAs in the bacterial lysate. The results in this study suggest that rSlNucAs expressed by the Baculovirus-B. mori protein expression system will be a useful tool in molecular biology. Functional recombinant protein of bacteria was produced by Baculovirus-B. mori protein expression system. This system may be highly suitable for bacterial extracellular protein secreted via Sec pathway..
15. Masato Hino, Takuji Kawanami, Jian Xu, Daisuke Morokuma, Kazuma Hirata, Mami Yamashita, Noriko Karasaki, Tuneyuki Tatsuke, Hiroaki Mon, Kazuhiro Iiyama, Noriho Kamiya, Yutaka Banno, Takahiro Kusakabe, Jae Man Lee, High-level expression and purification of biologically active human IL-2 using silkworm-baculovirus expression vector system., Journal of Asia-Pacific Entomology, 10.1016/j.aspen.2016.03.014, 19, 313-317, 2016.06.
16. Eigo Takahashi, Jae Man Lee, Hiroaki Mon, Yuuka Chieda, Chisa Yasunaga-Aoki, Takahiro Kusakabe, Kazuhiro Iiyama, Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa., Plasmid, 10.1016/j.plasmid.2016.03.001, 84-85, 44-50, 2016.03.
17. Erika Taira, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Chisa Yasunaga-Aoki, Kazuhiro Iiyama, Virulence of lipopolysaccharide-deficient mutants of Serratia liquefaciens toward the silkworm, Bombyx mori., Journal of Insect Biotechnology and Sericology, 10.11416/jibs.85.1_007, 85, 7-14, 2016.04.
18. Jian Xu, Pingbo Zhang, Takahiro Kusakabe, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Yutaka Banno, Daisuke Morokuma, Jae Man Lee, Comparative proteomic analysis of hemolymph proteins from Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-sensitive or -resistant silkworm strains during infections, Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 10.1016/j.cbd.2015.07.003, 16, 36-47, 2015.07.
19. Oumi Nishi, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu, Phylogenetic status and pathogenicity of Metarhizium majus isolated from a fruit beetle larva in Japan, Mycological Progress, 10.1007/s11557-015-1082-7, 14, 8, 58-58, 2015.08.
20. Kazuhiro Iiyama, Hiroaki Mon, Kazuki Mori, Takumi Mitsudome, Jae Man Lee, Takahiro Kusakabe, Kousuke Tashiro, Shin-Ichiro Asano, Chisa Yasunaga-Aoki, Characterization of KfrA proteins encoded by a plasmid of Paenibacillus popilliae ATCC 14706T., Meta Gene, 10.1016/j.mgene.2015.03.001, 4, 29-44, 2015.06.
21. Takumi Mitsudome, Hiroaki Mon, Jian Xu, Zhiqing Li, Jae Man Lee, Anandrao Ashok Patil, Atsushi Masuda, Kazuhiro Iiyama, Daisuke Morokuma, Takahiro Kusakabe, Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori., Insect Biochemistry and Molecular Biology, 10.1016/j.ibmb.2015.01.008, 58, 55-65, 2015.03.
22. Erika Taira, Kazuhiro Iiyama, Hiroaki Mon, Kazuki Mori, Taiki Akasaka, Kousuke Tashiro, Chisa Yasunaga-Aoki, Jae Man Lee, Takahiro Kusakabe, Draft genome sequence of entomopathogenic Serratia liquefaciens strain FK01., Genome Announcements, 2, 3, e00609-14-e00609-14, 2014.06.
23. Jian Xu, Takahiro Kusakabe, Kimiko Yamamoto, Yoshitaka Suetsugu, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Yutaka Banno, Kaito Yoshimura, Jae Man Lee, A novel third chromosomal locus controls susceptibility to Autographa californica multiple nucleopolyhedrovirus in the silkworm, Bombyx mori., Applied Microbiology and Biotechnology, 98, 7, 3049-3058, 2014.04.
24. Kazuhiro Iiyama, Masahiro Otao, Kazuki Mori, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Kousuke Tashiro, Shin-Ichiro Asano, Chisa Yasunaga-Aoki, Phylogenetic relationship of Paenibacillus species based on putative replication origin regions and analysis of an yheCD-like sequence found in this region., Bioscience, Biotechnology, and Biochemistry, 10.1080/09168451.2014.905188, 78, 891-897, 2014.05.
25. Takumi Mitsudome, Jian Xu, Yudai Nagata, Atsushi Masuda, Kazuhiro Iiyama, Daisuke Morokuma, Zhiqing Li, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe, Expression, purification, and characterization of endo-β-N-acetylglucosaminidase H using baculovirus-mediated silkworm protein expression system, Applied Microbiology and Biotechnology, 172, 8, 3978-3988, 2014.04.
26. Jae Man Lee, Xu Jian, Hiroaki Mon, Takumi Mitsudome, Atsushi Masuda, Kaito Yoshimura, Kazuhiro Iiyama, Yuuka Chieda, Takahiro Kusakabe, Baculovirus-mediated gene transfer systems in silkworm larvae using constitutive host promoters, Journal of Asia-Pacific Entomology, 10.1016/j.aspen.2013.10.009, 17, 1, 73-78, 2014.03.
27. Jian Xu, Hiroaki Mon, Takahiro Kusakabe, Zhiqing Li, Kazuhiro Iiyama, Atsushi Masuda, Takumi Mitsudome, Jae Man Lee, Establishment of a soaking RNA interference and Bombyx mori nucleopolyhedrovirus (BmNPV)-hypersensitive cell line using Bme21 cell, Applied Microbiology and Biotechnology, 10.1007/s00253-013-5279-x, 97, 24, 10435-10444, 2013.12.
28. Jian Xu, Kaito Yoshimura, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Takahiro Kusakabe, Jae Man Lee, Establishment of Caenorhabditis elegans SID-1-Dependent DNA Delivery System in Cultured Silkworm Cells, Molecular Biotechnology, 10.1007/s12033-013-9694-0, 56, 3, 193-198, 2014.03.
29. Oumi Nishi, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu, Comparison of the germination rates of Metarhizium spp. conidia from Japan at high and low temperatures, Letters in Applied Microbiology, 10.1111/lam.12150, 57, 6, 554-560, 2013.12.
30. Saki Imai, Zhiqing Li, Kazuhiro Iiyama, Yoshitaka Miyagawa, Masashi Toyoda, Akihiro Umezawa, Hiroaki Mon, Takahiro Kusakabe, Kaito Yoshimura, Jae Man Lee, Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system, Journal of Insect Biotechnology and Sericology, 82, 2, 39-44, 2013.06.
31. Kazuhiro Iiyama, Kazuki Mori, Hiroaki Mon, Yuuka Chieda, Jae Man Lee, Takahiro Kusakabe, Kosuke Tashiro, Shin-ichiro Asano, Chisa Yasunaga-Aoki, Susumu Shimizu, Draft Genome Sequence of Paenibacillus popilliae ATCC 14706T, Journal of Insect Biotechnology and Sericology, 82, 2, 45-48, 2013.06.
32. Xu Jian, Nagata Yudai, Mon Hiroaki, Li Zhiqing, Zhu Li, Kazuhiro Iiyama, takahiro kusakabe, JAE MAN LEE, Soaking RNAi-mediated modification of Sf9 cells for baculovirus expression system by ectopic expression of Caenorhabditis elegans SID-1, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 10.1007/s00253-013-4785-1, 97, 13, 5921-5931, 2013.07.
33. Kazuhiro Iiyama, Oumi Nishi, Hiroaki Mon, JAE MAN LEE, takahiro kusakabe, Asano Shin-ichiro, Chisa Yasunaga-Aoki, Susumu Shimizu, Phylogenetic analysis of Paenibacillus popilliae and its related taxa based on housekeeping genes., J. Insect Biotechnol. Sericol. , 10.11416/jibs.82.1_001, 82, 1-11, 2013.02.
34. Li Zhu, Zhiqing Li, Tsuneyuki Tatsuke, Daojun Cheng, Jian Xu, Kaito Yoshimura, Mon Hiroaki, Kazuhiro Iiyama, JAE MAN LEE, Qingyou Xia, takahiro kusakabe, Genome-wide identification of Argonaute 1- and Argonaute 2-regulating genes revealed an inhibition of macula-like virus by RNAi pathoway in the silkworm, Bombyx mori, Journal of Insect Biotechnology and Sericology, 10.11416/jibs.82.1_019, 82, 19-23, 2013.02.
36. Fukushima Mai, Kazuhiro Iiyama, Yamashita Jun, Furue Masutaka, Tsuji Gaku, Imanishi Shigeo, Mon Hiroaki, JAE MAN LEE, takahiro kusakabe, PRODUCTION OF SMALL ANTIBACTERIAL PEPTIDES USING SILKWORM-BACULOVIRUS PROTEIN EXPRESSION SYSTEM, PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 10.1080/10826068.2012.762717, 43, 6, 565-576, 2013.08.
37. Mon Hiroaki, JAE MAN LEE, Fukushima Mai, Nagata Yudai, Fujii Mie, Xu Jian, Nishi Oumi, Kazuhiro Iiyama, takahiro kusakabe, Production and characterization of the celery mismatch endonuclease CEL II using baculovirus/silkworm expression system, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 10.1007/s00253-012-4583-1, 97, 15, 6813-6822, 2013.08.
38. Arai Hiroyuki, Iiyama Kazuhiro, Role of nitric oxide-detoxifying enzymes in virulence of Pseudomonas aeruginosaagainst the silkworm, Bombyx mori, Biosci. Biotechnol. Biochem, 77, 1, 198-200, 2013.01.
39. Yasuhiko Soejima, Lee Jae Man, Yudai Nagata, Hiroaki Mon, Iiyama Kazuhiro, Hajime Kitano, Matsuyama Michiya, kusakabe takahiro, Comparison of signal peptides for efficient protein secretion in the baculovirus-silkworm system, CENTRAL EUROPEAN JOURNAL OF BIOLOGY, 10.2478/s11535-012-0112-6, 8, 1, 1-7, 2013.01.
40. Kaibara Fusako, Iiyama Kazuhiro, Chieda Yuuka, Lee Jae Man, kusakabe takahiro, Yasunaga-Aoki Chisa, Shimizu Susumu, Construction of serralysin-like metalloprotease-deficient mutants of Serratia liquefaciens and their virulence in the silkworm, Bombyx mori, J. Insect Biotechnol. Sericol. , 81, 55-61, 2012.12.
41. Lee Jae Man, Kawakami Noya, Hiroaki Mon, Mitsunobu Hitoshi, Iiyama Kazuhiro, Ninaki Satoshi, Maenaka Katsumi, Park Y Enoch, kusakabe takahiro, Establishment of a Bombyx mori nucleopolyhedrovirus (BmNPV) hyper-sensitive cell line from the silkworm e21 strain, Biotechnology Letters, 34, 10, 1773-1779, 2012.06.
42. Lee, JM., Mon, H., Banno, Y., Iiyama, K. and Kusakabe T. , Bombyx mori strains useful for efficient recombinant protein production using baculovirus vector. , J. Biotechnol. Biomaterial., S9, 003, 2012.02.
43. Chieda, Y., Iiyama, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S., Virulence of an exotoxin A-deficient strain of Pseudomonas aeruginosa toward the silkworm, Bombyx mori., Microbial Pathogenesis, 51, 6, 407-414, 2011.12.
44. Mon, H., Izumi, M., Mitsunobu, H., Tatsuke, T., Iiyama, K., Jikuya, H. Lee, J.M. and Kusakabe, T., Post-translational modificationsof theN-terminal tail of histone H3 in holocentric chromosomes of Bombyx mori., Insect Biochem. Mol. Biol., 41, 902-908, 2011.09.
45. Nishi, O., Hasegawa, K., Iiyama, K., Yasunaga-Aoki, C. and Shimizu, S. , Phylogenetic analysis of Metarhizium spp. isolated from soil in Japan., Appl. Entomol. Zool., 46, 301-309, 2011.08.
46. Iiyama, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S. , Improvement in pHERD vectors to express recombinant proteins tagged with hexahistidine at either the NH2- or COOH- terminal in Pseudomonas aeruginosa, J. Insect Biotechnol. Sericol., 80, 57-61, 2011.06.
47. Molecular characterization of core histones in the silkworm Bombyx mori. , Mitsunobu, H., Izumi, M., Iiyama, K., Jikuya, H., Lee, J.M., Mon, H., Kawaguchi, Y. and Kusakabe, T., J. Insect Biotechnol. Sericol., vol. 79, No. 3, 75-83, 2010.10.
48. Nishi, O., Iiyama, K., Yasunaga-Aoki, C. and Shimizu, S., Virulence of Metarhizium anisopliae var. majus isolated from soils against termite, Coptotermes formosanus Shiraki., Jpn. J. Environ. Entomol. Zool. , 21, 29-35, 2010.01.
49. Egami, I., Iiyama, K., Zhang, P., Chieda, Y., Ino, N., Hasegawa, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S., Insecticidal bacterium isolated from an ant lion larva from Munakata, Japan., J. Appl. Entomol., vol. 133 No. 2 117-124, 2009.03.
50. Iiyama, K., Chieda, Y., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S., Virulence of Phospholipase C Mutants of Pseudomonas aeruginosa PAO1 against the Silkworm, Bombyx mori., J. Insect Biotechnol. Sericol., vol. 77, No. 2, 115-120, 2008.06.
51. Chieda, Y., Iiyama, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S., Inactivation of pyocyanin synthesis genes has no effect on the virulence of Pseudomonas aeruginosa PAO1 toward the silkworm, Bombyx mori, FEMS Microbiology Letters, 278 101-107, 2008.01.
52. Chieda, Y., Iiyama, K., Lee, J.M., Kusakabe, T., Yasunaga-Aoki, C. and Shimizu, S., The gacA gene of Pseudomonas aeruginosa PAO1 is not required for full virulence in Bombyx mori., J. Insect Biotechnol. Sericol. , Vol. 76, 89-95, 2007.06.
53. Iiyama K, Chieda Y, Lee JM, Kusakabe T, Yasunaga-Aoki C, Shimizu S., Effect of superoxide dismutase gene inactivation on virulence of Pseudomonas aeruginosa PAO1 toward the silkworm, Bombyx mori, Appl Environ Microbiol., 73(5):1569-75, 2007.03.
54. Ura, H., Furuya, N., Iiyama, K., Hidaka, M., Tsuchiya, K., Matsuyama, N. , Burkholderia gladioli associated with symptoms of bacterial grain rot and leaf-sheath browning of rice plants, Journal of General Plant Pathology, 72 (2), pp. 98-103, 2006.04.
55. Chieda, Y., Iiyama, K., Yasunaga-Aoki, C., Lee, J.M., Kusakabe, T. and Shimizu, S., Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori, FEMS Microbiol. Lett., 10.1016/j.femsle.2005.01.032, 244, 1, 181-186, 244巻 181-186, 2005.03.
56. Ohsaki A, Iiyama K, Miyagawa Y, Kawaguchi Y, Koga K, Kusakabe T., Nonhomologous end-joining in a cell-free extract from the cultured silkworm, Mol Biol Rep., 10.1007/s11033-004-2474-y, 32, 1, 25-34, 32(1):25-34., 2005.03.
57. Seo, S.-T., Furuya, N., Iiyama, K., Takeshita, M., Takanami, Y., Tsuchiya, K., Lim, C.-K., Characterization of an antibacterial substance produced by Erwinia carotovora subsp. carotovora Ecc 32, Journal of General Plant Pathology, 70 (5), pp. 273-277, 2004.10.
58. Iiyama, K., Chieda, Y., Yasunaga-Aoki, C., Hayasaka, S. and Shimizu, S., Analyses of the ribosomal DNA region in Nosema bombycis NIS 001., J. Eukaryot. Microbiol, 10.1111/j.1550-7408.2004.tb00592.x, 51, 6, 598-604, 51巻 598-604., 2004.01.
59. Yasunaga-Aoki, C., Imanishi, S., Iiyama, K. and Kawarabata, T., Establishment of phagocytic cell lines from larval hemocytes of the beet armyworm, Spodoptera exigua., In Vitro Cell. Dev. Biol. Animal, 40, 7, 183-186, 40巻 183-186, 2004.01.
60. N. furuya, H. Ura, K. Iiyama, M. Matsumoto, M. Takeshita and Y. Takanami, Specific oligonucleotide primers based on sequences of the 16S-23S rDNA spacer region for the detection of Burkholderia gladioli by PCR, Journal of General Plant Pathology, vol. 68, No. 3 220-224, 2002.01.
61. S. Hayasaka, M. Nozaki, C. Yasunaga-Aoki, N. Teramoto, K. Iiyama, Y. Hatakeyama, M. Hashiguchi and T. Kawarabata, A new microsporidiosis occurring in larvae of Antheraea pernyi (Lepidoptera: saturniidae) released on an oak tree, Quercus acutissima and a possible insect-vector, Lepidogma kiiensis (Lepidoptera: pyralidae) inhabitating the same plant, Sericologia, vol 42 No. 1 107-111, 2002.01.
62. Inanaga, H., Kobayasi, D., Yoshiaki, K., Aoki-Yasunaga, C., Iiyama, K. and Kimura, M., Protein engineering of novel proteinase inhibitors and their effects on the Growth of Spodoptera exigua larvae., Biosci. Biotechnol. Biochem., 10.1271/bbb.65.2259, 65, 10, 2259-2264, vol. 65 2259-2264., 2001.01.
63. N. Furuya, T. Masunaga, AA Khan, K. Iiyama, M. Matsumoto and N. Matsuyama, Bacterial wild of Russell prairie gentian caused by Burkholderia caryophylli, Journal of General Plant Pathology, vol. 66 No. 4 316-322, 2000.01.
64. M. Kawanami, N. Furuya, K. Iiyama, K.H. Kim, S. Morimoto and N. Matsuyama, Purification and characterization of anti-Pholeospora maculans substance produced by Bacillus subtilis AB89., Ann. Phytopath. Soc. Japan, Vol. 65, 616-619, 1999.12.
65. M.S. Melo, N. Furuya, K. Iiyama, A.A. Khan and N. Matsuyama, Geographical distribution of biovars of Ralstonia solanacearum in Brazil., J. Fac. Agr., Kyushu Univ., 44, 1-2, 9-15, Vol. 44, 9-15, 1999.10.
66. Matsuyama, N., Ueda, Y., Iiyama, K., Furuya, N., Ura, H., Khan, A.A., Matsumoto, M., Rapid Extraction-HPLC as a Tool for Presumptive Identification of Burkholderia gladioli, B. glumae and B. plantarii, Causal Agents of Various Rice Diseases, J. Fac. Agr., Kyushu Univ., 42, 3-4, 265-272, 42 (3-4), pp. 265-272, 1998.03.
67. Iiyama, K., Furuya, N., Ura, H., Matsuyama, N., Role of Phytotoxins in the Pathogenesis of Burkholderia Species, J. Fac. Agr., Kyushu Univ., 42, 3-4, 289-293, 42 (3-4), pp. 289-293, 1998.03.
68. Furuya, N., Yamasaki, S., Nishioka, M., Shiraishi, I., Iiyama, K. and Matsuyama, N., Antimicrobial activities of Pseudomonads against plant pathogenic organisms and efficacy of Pseudomonas aeruginosa ATCC7700 against bacterial wilt of tomato, Ann. Phytopath. Soc. Japan, Vol. 63, 417-424, 1997.12.
69. Furuya, N., Iiyama, K., Shiozaki, N., Matsuyama, N., Phytotoxin Produced by Burkholderia gladioli, J. Fac. Agr., Kyushu Univ., 42, 1-2, 33-37, 42 (1-2), pp. 33-37, 1997.12.
70. Furuya, N., Iiyama, K., Ueda, Y., Matsuyama, N., Reaction of Tobacco and Rice Leaf Tissue Infiltrated with Burkholderia glumae or B. gladioli, J. Fac. Agr., Kyushu Univ., 42, 1-2, 43-51, 42 (1-2), pp. 43-51, 1997.12.
71. Iiyama, K., Furuya, N., Takanami, Y. and Matsuyama, N., A role of phytotoxin in virulence of Pseudomonas glumae Kurita et Tabei, Ann. Phytopath. Soc. Japan, Vol. 61, 470-476, 1995.10.
72. Iiyama, K., Furuya, N., Hara, K., Nakashima, N., Takanami, Y. and Matsuyama, N., Phytotoxin produced by Pseudomonas glumae Kurita et Tabei, a causal bacterium of the grain and seedling rot of rice., J. Fac. Agr., Kyushu Univ., 38, 3-4, 175-181, 38 (3-4), pp. 175-181, 1994.03.