Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Yasuhiro Utsumi Last modified date:2021.06.15

Associate Professor / Forest Sciences / Department of Agro-environmental Sciences / Faculty of Agriculture

1. Suzuki SN. Ataka M., Djukic, I., Enoki, T., Fukuzawa K., Hirota M., Hishi, T., Hiura, T., Hoshizaki, K., Ida, H., Iguchi, A., Iimura, Y., Ise, T., Kenta, T., Kina, Y., Kobayashi, H. Kominami, Y. Kurokawa, K., Makoto, K. Matsushita, M., Miyata, R., Muraoka, H., Nakaji, T., Sato, T., Nakamura, M., Niwa, S., Noh, NJ.,Seino, T., Shibata, H., Suzuki, RO., Takahashi, K., Tsunoda, T., Ustumi, Y., Watanabe K., Harmonized data on early stage litter decomposition using tea material across Japan, Ecological Research, 34, 575-576, 2019.09.
2. Mayumi Y. Ogasa, Kenichi Yazaki, Yasuhiro Utsumi, Naoko H. Miki, Kenji Fukuda, Kathy Steppe, Short-time xylem tension relaxation prevents vessel refilling and alleviates cryo-fixation artifacts in diffuse-porous Carpinus tschonoskii and Cercidiphyllum japonicum, Tree physiology, 10.1093/treephys/tpz072, 39, 10, 1685-1695, 2019.06, Xylem tension relaxation is an important procedure that closely resembles the in vivo xylem water distribution when measuring conductivity or observing water distribution of plant tissue samples by cryo-scanning electron microscopy (cryo-SEM). Recent studies have shown that partial xylem embolism occurs when samples under tension are cut under water and that gas-filled vessels are refilled during tension relaxation. Furthermore, the frequency of gas-filled vessels has been reported to increase in samples without tension relaxation before cryo-fixation by liquid nitrogen, particularly in samples with significant tension. Here, we examined the effect of tension relaxation on these artifacts in Carpinus tschonoskii and Cercidiphyllum japonicum using magnetic resonance imaging. We observed that xylem embolism rarely occurs in bench-dried samples cut under water. In both species, a small portion of the xylem was refilled within ~1 h after tension relaxation. Cryo-SEM observations revealed that short-time (<1 h) xylem tension relaxation decreases the frequency of gas-filled vessels in samples frozen after xylem tension relaxation regardless of the water potential compared with that in samples frozen without rehydration in both species. Therefore, short-time tension relaxation is necessary to retain xylem water distribution during sample preparation against artifacts..
3. Kenichi Yazaki, Mayumi Y. Ogasa, Katsushi Kuroda, Yasuhiro Utsumi, Peter Kitin, Yuzou Sano, Xylem water distribution in woody plants visualized with a cryo-scanning electron microscope, Journal of Visualized Experiments, 10.3791/59154, 2019, 148, 2019.06, A scanning electron microscope installed cryo-unit (cryo-SEM) allows specimen observation at subzero temperatures and has been used for exploring water distribution in plant tissues in combination with freeze fixation techniques using liquid nitrogen (LN2). For woody species, however, preparations for observing the xylem transverse-cut surface involve some difficulties due to the orientation of wood fibers. Additionally, higher tension in the water column in xylem conduits can occasionally cause artifactual changes in water distribution, especially during sample fixation and collection. In this study, we demonstrate an efficient procedure to observe the water distribution within the xylem of woody plants in situ by using a cryostat and cryo-SEM. At first, during sample collection, measuring the xylem water potential should determine whether high tension is present in the xylem conduits. When the xylem water potential is low (< ca. −0.5 MPa), a tension relaxation procedure is needed to facilitate better preservation of the water status in xylem conduits during sample freeze fixation. Next, a watertight collar is attached around the tree stem and filled with LN2 for freeze fixation of the water status of xylem. After harvesting, care should be taken to ensure that the sample is preserved frozen while completing the procedures of sample preparation for observation. A cryostat is employed to clearly expose the xylem transverse-cut surface. In cryo-SEM observations, time adjustment for freeze-etching is required to remove frost dust and accentuate the edge of the cell walls on the viewing surface. Our results demonstrate the applicability of cryo-SEM techniques for the observation of water distribution within xylem at cellular and subcellular levels. The combination of cryo-SEM with non-destructive in situ observation techniques will profoundly improve the exploration of woody plant water flow dynamics..
4. Yuko Yasuda, Yasuhiro Utsumi, Tsutomu Enoki, Shinya Koga, High frequency of discontinuous rings in evergreen and deciduous hardwood species in a temperate forest, Forest Ecology and Management, 10.1016/j.foreco.2018.08.041, 430, 526-532, 2018.12, Tree age derived from annual-ring counts provides fundamental information on forest stands and individual trees in temperate forests. However, discontinuous growth in the trunk may cause inaccuracies in annual-ring counts, and the effect of radial growth rate and interspecific differences on discontinuous ring formation have not been evaluated in the same forest stand. We investigated the occurrence of discontinuous rings at the base of 435 tree trunk disks of 16 evergreen and 15 deciduous hardwood tree species growing in a temperate forest of Japan. The effects of tree age and radial growth rate on frequency of discontinuous rings and their interspecific differences were evaluated by a generalized linear mixed-effect model. Discontinuous rings were observed in 29 of the 31 species in 330 individuals, which is about 75% of the total number of individuals examined. The number of discontinuous rings proportionally decreased with the growth rate and increased with the tree age. On the other hand, the effects of the growth rate were different among species. The majority of species at the study site contained frequent discontinuous rings in the trunk base. Annual-ring counts from a single core would lead to underestimation of tree ages in this case. Stem disks or increment cores taken in multiple directions at ground level would be useful for accurate estimation of tree age in temperate hardwood forests..
5. Djukic, I., Kepfer-Rojas. S., Schmidt, I. K., Larsen, K. S., Beier, C., Berg. B., Verheyen, K., TeaComposition, Early stage litter decomposition across biomes
, Science of the Total Environment , 628, 1369-1394, 2018.07.
6. Yasuda, Y., Utsumi, Y., Tan, X., Tashiro, N., Fukuda, K., Koga, S. , Suppression of growth and death of meristematic tissues in Abies sachalinensis under strong shading: Comparisons between the terminal bud, the terminally lateral bud and the stem cambium, Journal of Plant Research, 131, 817-825, 2018.06.
7. Kudo, K., Utsumi, Y., Kuroda, K., Yamagishi, Y., Nabeshima, E., Nakaba, S., Yasue, K., Takata. K. and Funada, R., Formation of new networks of earlywood vessels in seedlings of the deciduous ring- porous hardwood Quercus serrata in springtime, 32, 725-734, 2018.02.
8. Brett A. Bergman, Edward G. Bobich, Stephen D. Davis, Yasuhiro Utsumi, Frank W. Ewers, Dense but flexible wood-How leaf nodes impact xylem mechanics in Juglans californica, IAWA Journal, 10.1163/22941932-20170205, 39, 4, 372-381, 2018.01, A node is the point of attachment of the leaf to the stem of a plant; gaps associated with nodes have been viewed as discontinuities of the stem vascular system. We tested the hypothesis that the node/gap is a spring-like joint that impacts stem flexibility even well after the leaves have been shed, with some stems specialized for elongation and others for flexibility. Four-point bending tests were done using an Instron Mechanical Testing Device with the independent variable being the number of nodes in the stem segment and dependent variables being Modulus of Elasticity (MOE), Modulus of Rupture (MOR), and xylem density. Node anatomy was examined microscopically to assess structure and function. The stiffness of the stem was inversely proportional to the frequency of leaf nodes. Surprisingly, xylem density was inversely proportional to the frequency of leaf nodes in stems of adult trees. The tissue around nodes/gaps consisted of twisted and contorted cells that may be effective at absorbing compressive and tensile stresses. Because nodes behave as spring-like joints, the frequency of nodes relates to function, with some stems specialized for vertical expansion and others for light capture and damping of wind stress. The ultimate stems on a tree are the most bendable, which may allow the trees to avoid breakage..
9. Toshihiro Umebayashi, Toshimitus Morita, Yasuhiro Utsumi, Dai Kusumoto, Yuko Yasuda, Tomoyuki Haishi, Kenji Fukuda, Spatial distribution of xylem embolisms in the stems of Pinus thunbergii at the threshold of fatal drought stress, Tree Physiology, 36, 1210-1218, 2016.10.
10. Toshihiro Umebayashi, Yasuhiro Utsumi, Shinya Koga, Ikue Murata, Kenji Fukuda, Differences in drought-and freeze-induced embolism in deciduous ring-porous plant species in Japan, Planta, 30, 753-760, 2016.09.
11. Toshihiro Umebayashi, Mayumi Ogasa, Naoko Miki, Yasuhiro Utsumi, Tomoyuki Haishi, Kenji Fukuda, Freezing xylem conduits with liquid nitrogen creates artifactual embolisms in water-stressed broadleaf trees, Trees, 30, 305-316, 2016.02.
12. Mayumi Ogasa, Yasuhiro Utsumi, Naoko Miki, Kennichi Yazaki, Kenji Fukuda, Cutting stems before relaxing xylem tension induces artefacts in Vitis coignetiae, as evidenced by magnetic resonance imaging, Plant Cell and Environment, 39, 329-337, 2016.02.
13. Yuzou Sano, Yasuhiro Utsumi, Ryogo Nakata, Homoplastic occurrence of perforated pit membranes and torus-bearing pit membranes in ancestral angiosperms as observed by field-emission scanning electron microscopy
, Journal of Wood Science, 2013.04.
14. Sou N. Matsunaga, Shigeru Niwa, Tomoki Mochizuki, Akira Tani, Dai Kusumoto, Yasuhiro Utsumi, Tsutomu Enoki, Tsutom Hiura, Seasonal variation in basal emission rates and composition of mono- and sesquiterpenes emitted from dominant conifers in Japan, Atmospheric Environment , 69, 124-130, 2012.12.
15. Satoshi Nagai, Yasuhiro Utsumi, The function of intercellular spaces along the ray parenchyma in sapwood, intermediate wood and heartwood of Cryptomeria japonica (Cupressaceae), American Journal of Botany, 99, 1533-1561, 2012.08.
16. Sou N. Matsunaga, Satoru Chatani, Seiji Nakatsuka, Dai Kusumoto, Katsuyoshi Kubota, Yasuhiro Utsumi, Tsutomu Enoki, Tani Akira, Tsutomu Hiura, Determination and potential importance of diterpene (kaur-16-ene) emitted from dominant coniferous trees in Japan, Chemosphere , 87, 886-893, 2012.02.
17. Umebayashi, T., Koga, S., Utsumi, Y., Inoue, S., Shiiba, Y., Nagasawa, H., Osaki, S., KUbota, K., Inoue, S., Matsumura, J., Oda, K. and Otsuki, K., Green moisture content and basic density of 95 woody species growing in Kyushu University Forests, Japan, Bulletin of Kyushu University Forest, 92, 33-44, 2011.03.
18. Utsumi, Y., Bobich, E. G., Ewers, F. W., Photosynthetic, hydraulic and biomechanical responses of Juglans californica shoots to wildfire, Oecologia, 10.1007/s00442-010-1653-x, 2010.07.
19. Umebayashi, T., Utsumi, Y., Koga, S., Inoue, S., Fujikawa, S., Arakawa, K., Matsumura, J., Oda, K. and Otsuki, K., Xylem water-conducting patterns of 34 broadleaved evergreen trees in southern Japan, Trees, 10.1007/s00468-010-0428-7, 24, 571-583, 2010.04.
20. Murata, I., Saruki, S., Kubota, K., Inoue, S., Tashiro, N. Enoki, T., Utsumi, Y. and Inoue, S., Effects of sika deer (Cervus nippon) and dwarf bamboo (Sasamorpha borealis) on seedling emergence and survival in cool-temperate mixed forests in the Kyushu Mountains, Journal of Forest Research, 14, 296-301, 2009.10.
21. Kumagai, T., Aoki, S. Otsuki, K. and Utsumi, Y., Impact of stem water storage on diurnal estimates of whole-tree transpiration and canopy conductance from sap flow measurements in Japanese cedar and Japanese cypress trees, Hydrological Processes, 23, 2335–2344, 2009.07.
22. Tateishi, M., Kumagai, T., Utsumi, Y., Umebayashi, T., Shiiba, Y., Inoue, K., Kaji, K., Cho, K. and Otsuki, K, Spatial variations in xylem sap flux density in evergreen oak trees with radial-porous wood: comparisons with anatomical observations, Trees, 23:23-30, 2008.02.
23. Umebayashi, T., Utsumi, Y., Koga, S., Inoue, S., Shiiba, Y., Arakawa, K., Matsumura, J. and Oda, K., Optimal conditions for visualizing water-conducting pathways in a living tree by the dye injection method, Tree Physiology, 27: 993-999 , 2007.07.
24. Utsumi, Y., Koga, S., Tashiro, N., Yamamoto, A. Saito, Y. and Arima, T., The effect of bark decortication for hiwada production on xylem and phloem formation in Chamaecyparis obtusa, Journal of Wood Science, 52: 477-482, 2006.12.
25. Matsunaga, M., Shiratori, R., Matsumura, J., Oda, K., Utsumi, Y. and Yamamoto, K., Potassium distribution in black heartwood of sugi (Cryptomeria japnica) I: localization in axial parenchyma cells, Journal of Wood Science, 52: 95-100, 2006.04.
26. Kumagai, K. Aoki, S., Nagasawa, H. Mabuchi, T. Kubota, K. Inoue, S. Utsumi, Y. and Otsuki, K., Effects of tree-to-tree and radial variations on sap flow estimates of transpiration in Japanese cedar, Agricultural and Forest Meteorology, 10.1016/j.agrformet.2005.11.007, 135, 1-4, 110-116, 135: 110-116, 2005.01.
27. Kumagai, T., Nagasawa, H., Mabuchi, T., Ohsaki, S., Kubota, K., Kogi, K., Utsumi, Y., Koga, S. and Otsuki, K., Sources of error in stand transpiration estimate using relationship between stem diameter and sapwood area for Cryptomeria japonica and Chamaecyparis obtusa, Forest Ecology and Management, 10.1016/j.foreco.2004.10.066, 206, 1-3, 191-195, 206: 191-195, 2005.01.
28. Hirose, S, Kume, A., Takeuchi, S., Utsum, Y. Otsuki, K. and Ogawa, S., Stem water transport of Lithocarpus edulis, an evergreen oak with radial-porous wood, Tree Physiology, 25, 2, 221-228, 25: 221-228, 2005.01.
29. Sano, Y, Okamura, Y. and Utsumi, Y., Visalizing water-conduction pathways of living trees: selection of dyes and tissue preparation methods, Tree Physiology, 25: 269-275, 2005.01.
30. Abe, H., Nakai, T., Utsumi, Y. and Kagawa, A., Temporal water deficit and wood formation in Cryptomeria japonica, Tree Physiology, 23, 12, 859-863, 23: 859-863, 2003.01.
31. Utsumi, Y., Sano, Y., Funada, R., Ohtani, J. and Fujikawa, S., Seasonal and perennial changes in the distribution of water in the sapwood of conifers in a subfrigid zone, Plant Physiology, 10.1104/pp.102.014795, 131, 4, 1826-1833, 131: 1826-1833, 2003.01.
32. Utsumi, Y., Sano, Y., Funada, R., Fujikawa, S. and Ohtani, J., The progression of cavitation in earlywood vessels of Fraxinus mandshurica var. japonica during freezing and thawing, Plant Physiology, 121: 897-904., 1999.01.
33. Utsumi, Y., Sano, Y., Ohtani, J. and Fujikawa, S., Seasonal changes in the distribution of water in the outer growth rings of Fraxinus mandshurica var. japonica: A study by cryo-scanning electron microscopy, IAWA Journal, 1996.01.