| 1. |
Toshihiro Ona,Junko Johzuka, Rapid and synchronized dormancy-breaking jumbo leek bulb inhibits postprandial carbohydrate degradation and absorption to be assumed preprandial ingestion, MDPI, 10.3390/ECMC2022-13270, Medicinal Sciences Forum. Vol. 14: 63, 2022.12, In order to prevent obesity and diabetes, it is important to avoid overeating, especially carbohydrates. Therefore, we evaluated the effect of taking jumbo leek bulb before a meal on the decomposition and absorption inhibition of carbohydrates after a meal. In this case, we used a product that was heat-treated after promoting phase transition of its content by rapid and synchronized dormancy-breaking (RSDB), and investigated by decreasing two enzyme activities, namely pancreatic α-amylase and α-glucosidase, and report the results.. |
| 2. |
小名 俊博,𠀋束 純子, 迅速・同時休眠打破したジャンボリーキ鱗片末の食前摂取を想定した食後の糖質分解・吸収抑制効果, ICNIM2022実行委員会, 103-106, 2022.08, 肥満・糖尿病予防のため、ジャンボリーキ鱗片末を食前に摂取した場合の食後の糖質の分解・吸収抑制効果を、膵α-アミラーゼとα-グルコシダーゼの二つの酵素活性の低下により検討した。この際、試料を迅速・同時休眠打破により内容成分の相転移を促した後加熱処理し用いた。. |
| 3. |
Toshihiro Ona,Junko Johzuka, Liver mitochondrial activation and fat-lowering effects of rapid and synchronized dormancy-breaking ginger rhizome assumed to be administered orally, MDPI, 10.3390/ECMC2021-11507, 7th International Electronic Conference on Medicinal Chemistry session Round Table on Natural Products, 2021.11, [Background/Aims] In ginger rhizome, the percentage of 6-gingerol (GING) is high before heating and the percentage of 6-shogaol (SHOG) increases after heating, and SHOG has been reported to activate liver metabolism. We have studied the method of rapid and synchronized dormancy-breaking treatment with Awakening liquidTM to promote phase transition of content components in seeds, resulting in improved functionality. In this study, we applied this method to ginger rhizome. After the treatment and heating, we evaluated liver activation and final fat-reduction before and after metabolism in the liver, assuming oral administration, including comparison between GING and SHOG.
[Results] At 100nM, apoptotic toxicity was observed in GING both before and after metabolism, and promotion of metabolic activity was observed in SHOG, about 2 times greater after metabolism than before. In SHOG 1000nM, apoptotic toxicity was observed before metabolism, and promotion of metabolic activity was observed after metabolism by detoxification. The SHOG 100 nM decreased liver fat by about 20%. In dormancy-breaking ginger rhizome endosperm, SHOG was found to increase, containing about 8 times more than untreated and about 1.5 times more than non-dormancy-breaking one. There was also a concentration-dependent decrease in liver fat, which was about 27% at 2.0 mg/ml. However, apoptotic toxicity was observed at 3.0 mg/ml and above. Similarly, untreated sample showed little effect at 1.0 mg/ml, and apoptotic toxicity was observed at 2.0 mg/ml and above. In the model experiment assuming oral administration, rapid and synchronized dormancy-breaking ginger rhizome showed high effects on liver mitochondrial activation and fat lowering.. |
| 4. |
Junko Johzuka,Toshihiro Ona,, One hour in vivo-like phenotypic screening system for anti-cancer drugs using a high precision surface plasmon resonance device, MDPI, 10.3390/ECMC2020-07366, 6th International Electronic Conference on Medicinal Chemistry session Round Table on Natural Products, 2020.11, [Background/Aims] In anti-cancer drug (candidate) screening, the demand exists for evaluation at physiological concentrations similar to in vivo. This is often performed by three-dimensionally (3D) cultured cells. It necessitates a long culture period of 2-4 weeks with tedious experimental procedures based on endpoint assay and labeling agents causing low reliability. The previous device methods depend on the pharmaceutical mode of action, little related to the conventional method. Furthermore, a separate set of experiment is required to obtain both efficacy and toxicity. Here, we report on a high precision surface Plasmon resonance-3D system to overcome these all problems sensing dynamic cellular reaction against target compound(s) by laser.
[Results] We developed the system with average fluctuation of 50 ndeg/s combined with two-dimensionally cultured cells attached onto a sensor chip by applying collagen on the top. The 3D cell activity was shortly obtained by this without cell division. New system gave real-time monitoring of mitochondrial membrane potential (MMP) within live cells without both labeling and invasion. It allowed in vivo-like phenotypic screening for anti-cancer drugs within 1h of drug addition. The data were collected as the stable linear signal change parts for at least 5min after 25min following drug addition. The results provided compatibility to clinically related chemosensitivity test (P |
| 5. |
Toshihiro Ona,Junko Johzuka, Rapid and synchronized dormancy-broken Kyoho grape seed endosperm without extraction or concentration shows significant effect on both anti-cancer and pro-immunity, MDPI, 10.3390/ECMC2020-07249, 6th International Electronic Conference on Medicinal Chemistry session Round Table on Natural Products, 2020.11, [Background/Aims] Anti-cancer effect has been reported in oral administration of grape seed extract (GSE) although the clinical trial failed because of its toxicity. Grape seeds should be toxic to prevent them to be eaten by animals. However, once they wake up, they change the ingredients into a form which dissolves easily in water and break toxicity substances inhibiting germination. In order for seeds to sprout and grow, a lot of nutrients as well as life itself reside. The objective of this study is to determine the in vivo-like efficacy and toxicity on anti-cancer and pro-immunity of rapid and synchronized dormancy-broken grape seed endosperm (RSDB-GSE) by our proprietary Grandir recipe. It was performed in case of oral administration using digested and absorbed RSDB-GSE.
[Results] Direct killing efficacy against all cancer cells was observed in a dose-dependent manner at 2.5 mg/ml or more, and it was the maximum at 5.0 mg/ml for pancreas and liver and at 7.5 mg/ml for breast. The cell viability was less than 20%. At concentrations higher than these, it showed toxicity which was thought to be cell functional shutdown by suspension of mitochondrial polarization. No effect was observed for normal cells. The RSDB-GSE showed general direct cancer killing efficacy for organs. T lymphocytes cytotoxic activity by NK cells was observed in a dose-dependent manner at 3.75 mg/ml or more, and it was kept at least until 5.0 mg/ml. From this, pro-immunity was shown by RSDB-GSE. Consequently, it is expected to express synergistic anti-cancer effect, even orally administered.. |
| 6. |
小名 俊博,𠀋束 純子, 迅速・同時休眠打破巨峰ブドウ種子胚乳のヒト乳がんならびに肝臓がんに対する直接殺傷効果, ICNIM2019実行委員会, 163-165, 2019.08, 一昨年度、昨年度の本会において、国内産の巨峰からタネを取り出し、これを休眠打破することにより内容成分の相転移を促し、経口投与を想定したモデル実験について発表した。休眠打破した巨峰ブドウ種子胚乳の膵臓がんに対する高い直接殺傷効果と低い毒性、ならびに高い免疫活性化効果であった。今回は、乳がん、および肝臓がんに対する直接殺傷効果について検討したので報告する。. |
| 7. |
小名 俊博,𠀋束 純子, 迅速・同時休眠打破ブドウ種子胚乳の免疫活性化効果, ICNIM2018実行委員会, 159-161, 2018.08, 迅速・同時休眠打破ブドウ種子胚乳の免疫活性化効果. |
| 8. |
小名 俊博,𠀋束 純子, 休眠打破ブドウ種子サプリメントの膵がんに対する薬効・毒性の評価, ICNIM2017実行委員会, 205-207, 2017.08, 休眠打破ブドウ種子サプリメントの膵がんに対する薬効・毒性の評価. |
| 9. |
小名 俊博, 柴田 純子, 最前線センサーシステムである表皮角化細胞ミトコンドリアの利用による共通皮膚刺激試験法の開発, ICNIM2016実行委員会, 227-229, 2016.08, 最前線センサーシステムである表皮角化細胞ミトコンドリアの利用による共通皮膚刺激試験法の開発. |
| 10. |
小名 俊博, 柴田 純子, 早川 明夫, 坂本 伸也, 佐賀県産ゴールドキウイフルーツの表皮ターンオーバー促進効果、並びに毒性の迅速in vivo-likeフェノタイピック アッセイ, ICNIM2015実行委員会, 181-183, 2015.08, 佐賀県産ゴールドキウイフルーツの表皮ターンオーバー促進効果、並びに毒性の迅速in vivo-likeフェノタイピック アッセイ. |
| 11. |
Junko - Shibata, Shouhei Yano, Teruyuki Seino, Toshihiro - Ona, Anti-liver cancer effect of Chinese chive using in vivo-like test, Organizing Committee of ICNIM2014, 227-229, 2014.08, Chinese chive is a perennial herb belonging to Allium genus cultivated BC era in China. In Japan, it is introduced in old book Manyoshu in 8th century. In herbal medicine, dried leaves of Chinese chive are used as called Kyusai. The efficacy spectrum is widely reported in tonicity, recover from exhaustion, improvements in vascular flow and cholesterol, preventions in arteriosclerosis and myocardial infarct, pro-body temperature, prevention and suppression in tumors, anti-microbial and so on.
As active compounds, Chinese chive contains sulphuric amino compounds of methin and aliin, vitamins C, A and E, minerals of Ca、K、Mg、Se、Fe、P, chlorophylls and dietary fibers.
In Japan, Chinese chive is cultivated from Hokkaido to Okinawa. In Hokkaido, Shiriuchi town in Oshima district is top for the production using a brand name of “Kitano Hana” with 1,700t/y. The first picking leaves are thick and sweat and attract customers with increased handling volume. Shiriuchi town is eager to add more values to “KitanoHana”and has started various projects.
On the other hand, in a liver, absorbed food compounds are directly works against a liver first. After this, metabolized compounds within a liver with chemical modification work against a liver secondary.
In active compound evaluation, physiological concentration results are demanded equally as in vivo. For this, in vivo-like chemosensitivity test is often hired using 3D cell culture with extracellular matrix. However, this requires time-consuming experimental process with 2-4 weeks and labeling agents causing misjudge, and has difficulty in simultaneous efficacy and toxicity measurement. We conquered all these problems with development of High Precision-Surface Plasmon Resonance (HP-SPR) device and applications.
Here we examined and conformed the nature of extract from Chinese chive for anti-cancer efficacy and toxicity against liver before and after their metabolism within liver as real-time manner by HP-SPR.. |
| 12. |
Toshihiro - Ona, Junko - Shibata, Activation of skin by AHCC: comparison between whole and low molecular weight fraction, Organizing Committee of ICNIM2014, 173-175, 2014.08, Skin aging has intrinsic and extrinsic processes. Intrinsic process is derived from genetic factors such as chronicle age and reduction in estrogen. Extrinsic one is from environmental factors such as exposure to sunlight and pollutants, and diet. These generate free radicals (ROS). When ROS amount exceeds cell scavenging capacity, the oxidative stress occurs.
The oxidative stress at epidermis accelerates the formation of Advanced Glycation End Products (AGE) by protein glycation of plasma membrane. AGE inhibits SOD activity, reduces DNA repair ability and stability, mitochondrial function, cell division and delays cell cycle and migration, and induces apoptosis. This implies the decrease in cell metabolism.
As a result, epidermis becomes thin with 10-50% reduction by atrophy of stratum spinosum. Furthermore, the skin glycation reduces skin elasticity. These increase skin vulnerability and fragility and decrease in desquamation to delay wound healing and in replacement speed of lipids to disturb barrier function.
The compound screening for epidermis activation often hires cocultivation of keratinocyte with a target compound. On the other hand, 3D cell culture is reported with the use of extracellular matrix and even with fibroblast cells from dermis to reconstitute skin. However, this necessitates long time culture duration (ca. 1 month) because of endpoint method, and has difficulty in simultaneous determination of efficacy and toxicity. Moreover, the reduction in cell activity causes low experimental repeatability by long term culture.
We developed a custom-made instrument and method to monitor early stage of mitochondrial response to stimuli within live cells utilizing High-Precision Surface Plasmon Resonance (HP-SPR) as a measurement principle. This brings endpoint efficacy and toxicity predictions extremely before endpoint without cell culture.
At last ICNIM meeting, we elucidated the validity of AHCC for pro-skin turnover. This time, we report on the validity difference by comparison between whole and low molecular weight fraction of AHCC.. |
| 13. |
Toshihiro - Ona, Activation of skin by AHCC, ICNIM2013実行委員会, 7-10, 2013.08, We successfully developed a rapid, high sensitive, label-free and non-invasive cell-based sensing technology for reliable efficacy prediction of bioactive compounds at physiological concentration within 1 h regardless to the mode of action. Conventional technology is based on endpoint assay of 3D cell culture with extracellular matrix necessitating time-consuming experimental process and labeling agents causing interference with a target compound that provide low reliability. The conventional device method depends on the pharmaceutical mode of action, and gives no relation to the conventional method. New technology overcomes these all problems. Expected applications for compound screening are; 1. Anti-cancer, -Alzheimer disease, -aging (skin care), diabetes2. Pro-fat burning, -metabolism, -hair restoration, -body temp. |
| 14. |
小名 俊博, 生理的濃度での肌のターンオーバー並びに毒性の迅速定量評価‐1時間で判定, FRAGRANCE JOURNAL, Vol. 41 (7): 87, 2013.07, 生理的濃度での肌のターンオーバー並びに毒性を生細胞応答により検出、作用機序に左右されず、投与後1時間以内で、迅速、簡便、非標識、かつ高感度で、確実に評価するシステムを開発した。従来法にあった長い判定期間や蛍光色素と成分との競合反応による誤判定、標準法の結果との相関低、多剤併用効果の評価難などを全て克服した。. |
| 15. |
小名俊博, 日本薬学会第132年会講演ハイライト「光により抗がん剤効果、生体濃度で迅速評価」, 公益財団法人 日本薬学会 広報委員会, p. 42, 2012.03. |
| 16. |
小名俊博, 生細胞内ミトコンドリアの光判定技術を用いた高信頼な機能性成分評価, 財団法人 九州大学学術研究都市推進機構, Vol. 26, p. 6, 2012.01. |
| 17. |
小名俊博, 平成22年度-新産業支援プラットフォーム事業-「生細胞のリアルタイムモニターのためのナノバイオデバイス」研究会 第三回セミナー講演集, 財団法人 九州産業技術センター, 29-33, 2011.02. |
| 18. |
小名俊博, 平成22年度-新産業支援プラットフォーム事業-「生細胞のリアルタイムモニターのためのナノバイオデバイス」研究会 第二回セミナー講演集, 財団法人 九州産業技術センター, 2010.11. |
| 19. |
小名俊博, 平成22年度-新産業支援プラットフォーム事業-「生細胞のリアルタイムモニターのためのナノバイオデバイス」研究会 第一回セミナー講演集, 財団法人 九州産業技術センター, 2010.10. |
| 20. |
小名俊博, 日本分析化学会第58年会「展望とトピックス」－レーザー光で抗がん剤の効き目を迅速に見分ける－, 日本分析化学会 展望とトピックス委員会, 15, 2010.01. |
| 21. |
Ona, T., Nishijima, H., Kosaihira, A. and Shibata, J., Development of cell-based quantitative evaluation method for cell cycle-arrest type cancer drugs for apoptosis by high precision surface plasmon resonance sensor, SPIE, Biophotonics: Photonic Solutions for Better Health Care-Progress in Biomedical Optics and Imaging, Vol. 9 (38), Eds. Popp, J., Drexler, W., Tuchin, V.V. and Matthews, D.L., 69910R, 2009.04. |
| 22. |
小名俊博, あなたのミトコンドリアは元気ですか？, 荒川化学工業株式会社, 荒川ニュース、No. 342: 9, 2008.07. |
| 23. |
小斉平 篤、柴田 純子、小名俊博, 第６回 生体医用工学研究会 講演論文集, 日本光学会（（社）応用物理学会), 326-327, 2007.11. |
| 24. |
Kosaihira, A., Fukumori, T., Sakai, K. and Ona, T., Improvement of forest resources for recyclable forest products, Springer-Verlag, 170-172, A new combination device comprised of surface plasmon resonance and fluorescence microscopy for a rapid screening of anticancer phenolic compounds., 2004.03. |
| 25. |
Ibuki, T., Tokida, Y., Matsuda, N., Czempinski, K., Muller-Roeber, B., Ona T. and Uozumi, N., Improvement of forest resources for recyclable forest products, Springer-Verlag, 167-169, Characterization of potassium channel from Arabidopsis thaliana., 2004.03. |
| 26. |
Yokota, S., Nakayama, K., Sagawa, A., Urabe, F., Ona, T., Asada, T. and Yoshizawa, N., Improvement of forest resources for recyclable forest products, Springer-Verlag, 161-162, Possible effects of properties in polyphenol oxidases on rooting ability of Eucalyptus camaldulensis cutting shoots., 2004.03. |
| 27. |
Yamamoto, H., Kojima, Y., Okuyama, T., Ona, T. and Okayama, T., Improvement of forest resources for recyclable forest products, Springer-Verlag, 139-143, An essay on the fine structure of the wood cell wall related to the physical properties of the recycled paper., 2004.03. |
| 28. |
Murakami, S., Ona, T., Sakai, K., Saito, K. and Fukushima, K., Improvement of forest resources for recyclable forest products, Springer-Verlag, 111-116, Effect of deuterium exchange in lignin on its structural analysis using FT-Raman spectroscopy., 2004.03. |
| 29. |
Mizumoto, M., Seino, T., Sakai K., Ona, T., Ishida, Y. and Ohtani, H., Improvement of forest resources for recyclable forest products, Springer-Verlag, 107-110, Rapid characterization of total fatty acids in wood by reactive thermal desorption-gas chromatography with tetrabutylammonium hydroxide., 2004.03. |
| 30. |
Haisaki, H., Tateishi, M., Seino, T., Sakai, K., Ona, T., Ohshima, J., Adachi, K., Yokota S. and Yoshizawa, N., Improvement of forest resources for recyclable forest products, Springer-Verlag, 100-104, Assessment of vessel anatomical feature in Eucalyptus camaldulensis by pyrolysis-gas chromatography., 2004.03. |
| 31. |
Tateishi, M., Seino, T., Sakai, K., Ona, T., Ohshima, J., Adachi, K., Yokota, S. and Yoshizawa, N., Improvement of forest resources for recyclable forest products, Springer-Verlag, 95-99, Rapid assessment of vessel morphology by pyrolysis-gas chromatography., 2004.03. |
| 32. |
Ohshima, J., Yokota, S., Yoshizawa, N. and Ona, T., Improvement of forest resources for recyclable forest products, Springer-Verlag, 89-94, Representative heights assessing whole-tree values and the within-tree variations of derived wood properties in Eucalyptus camaldulensis and E. globulus., 2004.03. |
| 33. |
Ohshima, J., Adachi, K., Yokota, S., Yoshizawa, N. and Ona T., Improvement of forest resources for recyclable forest products, Springer-Verlag, 83-88, Within-tree variation of vessel morphology and frequency and representative heights for estimating the whole-tree values in Eucalyptus camaldulensis and E. globulus., 2004.03. |
| 34. |
Ohshima, J., Yokota, S., Yoshizawa, N. and Ona, T., Improvement of forest resources for recyclable forest products, Springer-Verlag, 77-82, Within-tree variation of detailed fiber morphology and its position representing the whole-tree value in Eucalyptus camaldulensis and E. globulus., 2004.03. |
| 35. |
Ishiguri, F., Yokota, S., Yoshizawa, N. and Ona, T., Improvement of forest resources for recyclable forest products, Springer-Verlag, 74-76, Radial variation of cell morphology in three acacia species., 2004.03. |
| 36. |
Yoshizawa, N., Ishiguri, F., Yokota, S. and Ona, T., Improvement of forest resources for recyclable forest products, Springer-Verlag, 71-73, Formation and structure of reaction wood fibers forming no G-layer in some hardwood species., 2004.03. |
| 37. |
Okayama, T., Kibatani, Y., Nakayama, K., Kojima Y. and Ona, T., Improvement of forest resources for recyclable forest products, Springer-Verlag, 52-59, Fiber properties and papermaking potential of recycled Acacia pulp., 2004.03. |
| 38. |
Seino, T., Yoshioka, A., Takai, M., Kojima, Y., Ishikura, Y., Ona, T., Ishida, Y. Ohtani, H. and Tsuge S., Improvement of forest resources for recyclable forest products, Springer-Verlag, 47-51, Characterization of photo-yellowing trigger compounds repressing paper recyclability of Eucalyptus globulus by Pyrolysis-GC/MS., 2004.03. |
| 39. |
Kojima, Y., Isaji, S. and Ona T., Improvement of forest resources for recyclable forest products, Springer-Verlag, 40-46, Production of high brightness CTMP from Eucalyptus globulus and their light-induced color reversion., 2004.03. |
| 40. |
Ona, T., Kawana, J., Kibatani, Y., Ishikura, Y., Kojima, Y. and Okayama, T., Improvement of forest resources for recyclable forest products, Springer-Verlag, 29-32, Toward the construction of efficient link between forest recycling and paper recycling using trees with high performance for paper recycling., 2004.03. |
| 41. |
Ona, T., Tateishi, M., Nozaki, H., Seino, T., Yoon, S-L., Isaji, S. and Kojima, Y., Improvement of forest resources for recyclable forest products, Springer-Verlag, 24-28, Feasibility Study of Tree Selection for High Pulp Yield, Brightness and Recyclable Chemithermomehanical Paper Production using Eucalyptus globulus.
, 2004.03. |
| 42. |
Ona, T., Improvement of forest resources for recyclable forest products, Springer-Verlag, 3-7, Overview of the project “Development of forest resources with high performance for paper recycling.
, 2004.03. |
| 43. |
Kosaihira, A., Fukumori, T., Sakai, K. and Ona, T. , 10th European Conference on the Spectroscopy of Biological Molecules, JATEPress , 71, Rapid screen of antiproliferative phenolic compounds with simultaneous analysis of cancer cell reaction by a new combination device of surface plasmon resonance and fluorescence microscopy., 2003.08. |
| 44. |
Ona, T., Ohshima, J., Adachi, K., Yokota, S. and Yoshizawa, N. , 10th European Conference on the Spectroscopy of Biological Molecules, JATEPress , 200a, In situ determination of vessel element length in tree by Fourier transform Raman spectroscopy. , 2003.08. |
| 45. |
T. Ona, T. Sonoda, K. Ito, M. Shibata, Y. Ootake, J. Ohshima, S. Yokota and N. Yoshizawa, Spectroscopy of Biological Molecules: New Directions, Kluwer Academic Publishers, 545-546 In situ determination of proportion of cell types in wood by FT-Raman spectroscopy, 1999.08. |
