|kuniyoshi shimizu||Last modified date：2022.06.28|
Associate Professor / Systematic Forest and Forest Products Science / Department of Agro-environmental Sciences / Faculty of Agriculture
|kuniyoshi shimizu||Last modified date：2022.06.28|
|1.||Mittraphab Yanisa, Kuniyoshi Shimizu, Anti-phototoxicity of Phenolic compounds from Entada phaseoloides leaves in Human Epidermal Keratinocytes, 11th Asian Natural Product Conference (Q-AOS), 2022.06.|
|2.||Ahmed Othman, Kuniyoshi Shimizu, Possible neuroprotective effect of amide alkaloids from some halophytes growing in Egypt, 11th Asian Natural Product Conference (Q-AOS), 2022.06.|
|3.||Kuniyoshi Shimizu, Ganoderma Triterpenoids: Hint for Understanding Multi-Medicinal effects of Ganoderma mushroom, International Congress of SOCIETY FOR ETHNOPHARMACOLOGY, INDIA 2022, 2022.04, Lingzhi (in Chinese) or Reishi (in Japanese), recently identified as Ganoderma lingzhi which is different from Ganoderma lucidum originally described from Europe, has been treasured for its medicinal value in traditional medicine in Japan, China and Korea for thousands of year. Of all Ganoderma mushrooms, which account for more than 300 known triterpenoids, G. lingzhi has been the most well-studied species. G. lingzhi was reported with a wide range of bioactivities including antioxidative, anti-aging, immunomodulatory activity, anti-hepatotoxic, ant-angiogenic, anti-histaminic, potential anticancer activity and even anti-HIV. Our group has isolated many triterpenoids from reishi and reported their bioactivities, including inhibition of benign prostatic hypertrophy, anti-androgenic osteoclastogenesis, anti-diabetes, anti-diabetic complication, antihypertension, inhibition of neuraminidase and anti-cancer activities. Following topics will be discussed.
1) Mushroom sample and triterpenoids isolation
2) 5-reductase inhibition and anti-benign prostate hyperplasia
3) Inhibition of osteoclast differentiation (anti-osteoporosis)
4) Inhibition of α-glucosidase (anti-diabetes)
5) Inhibition of aldose reductase (anti-diabetic complication)
6) Inhibition of angiotensin-converting enzyme (anti-hypertension)
7) Inhibition of neuraminidase (anti-influenza)
8) Inhibition of Rho kinase
9) Tumor cell cytotoxicity (anti-cancer)
10) Clues to pharmacological mechanisms (identification of target protein)
11) LC-MS/MS analysis for evaluating functional-quality of Ganoderma mushroom
Up to now, more than 300 triterpenoids have been reported from Ganoderma mushroom, especially from G. lingzhi. Also, many bioactivities of these compounds have been investigated. Potential application of this mushroom and the compounds in it on three indications including cancer, diabetes, and immune stimulation was also thoroughly examined. However, future randomized controlled clinical trials are still necessary. Despite of lacking of enough evidence from clinical trials, this mushroom remains a popular agent in commercial products. From this fact, studies on the Ganoderma mushroom should be still a hotter topic in the near futures.
|4.||Kuniyoshi Shimizu, Utilization of natural resources based on scientific evidence for sustainable development-pharmaceutical, functional food, aroma, living environment, Tunisia-Japan Symposium-Materials for Sustainable Development: Fundamentals & Applications, 2022.03.|
|5.||Ran,Suzuki,Wang Dongmei,Yhiya Amen,Naomichi Takemoto,Fatima Ezzahra Janati Idrissi,Tarik Ouchbani,Mitsutoshi Nakajima, Hiroko Isoda,Kuniyoshi Shimizu, An analytical method for argan saponins in argan oil by using liquid chromatography quadrupole time-of-flight mass spectrometry, Tunisia-Japan Symposium-Materials for Sustainable Development: Fundamentals & Applications , 2022.03.|
|6.||Kuniyoshi SHIMIZU, Utilization of natural resources for sustainable development based on scientific evidence-Use for pharmaceuticals, functional foods, aromas, living environment-, 1st International Conference on Highland Germpalsm (ICHG) -Technological Innovation and Sustainable Development Based on Highland Germplasm-, 2022.02.|
|7.||Kuniyoshi SHIMIZU, How should we utilize natural resources for sustainable development based on scientific evidence?-Use for pharmaceuticals, functional foods, aromas, living environment-, International Conference-Natural Resources Governance Toward Sustainable Development, 2021.11.|
|8.||Thien Huu Nguyen, Yhiya Amen, Dongmei Wang, Ahmed Othman, Kuniyoshi Shimizu, Characterization and Kinetic Studies of Proanthocyanidin Oligomers Complex Derived from Avocado Seed As a Promising Antidiabetic Agent, 8th Asian Natural Product Conference (Q-AOS), 2021.10.|
|9.||Asmaa Kamal El-Deen, Kuniyoshi Shimizu, Suspect and Non-target Screening of Emerging Contaminants in Wastewater Using LC-QTOF/MS in Data Independent Acquisition (DIA) Mode: Occurrence, Fate and Risk Assessment, 8th Asian Natural Product Conference (Q-AOS), 2021.10.|
|10.||Duanyang Wang, Maki Nagata, Masako Matsumoto, Yhiya Amen, Dongmei Wang, Kuniyoshi Shimizu, Bio-Activity Evaluation of Hibiscus sabdariffa Linn. in Skin Care and Isolation of Contributory Component, Kyushu University Asia Week 2021, 2021.10.|
|11.||Yanisa Mittrapha, Maki Nagata, Massako Matsumoto, Kuniyoshi Shimizu , Protective effects of acetone extract from Entada phaseoloides leaves on UVB-irradiated HaCaT keratinocytes, Kyushu University Asia Week 2021, 2021.10.|
|12.||Ruan Yang, Yanisa Mittraphab, Ahmed Muhamed Othman, Yhiya Amen, Wang Dongmei, Han Chunguang, Mika Abe, Kuniyoshi Shimizu , The Isolation and Identification of Hericenones in Hericium erinaceus (Yamabushitake) , Kyushu University Asia Week 2021, 2021.10.|
|13.||Ahmed Othman, Yhiya Amin, Masako Matsumoto, Maki Nagata, Kuniyoshi Shimizu, Utilization of Halophytes as a Source of Pharmaceutical Candidates, Kyushu University Asia Week 2021, 2021.10.|
|14.||Jiaxin Fan,Ahmed Ashour, Ahmed Hassan, Yhiya Amen, Maki Nagata, Masako Matsumoto, Shuhei Kaneko, Dongmen Wang, Satoshi Ishikawa, Shinich Muta, Kuniyoshi Shimizu, The expansion of the promising value of Flammulina velutipes and the prospect of its sustainable development, Kyushu University Asia Week 2021, 2021.10.|
|15.||Asmaa Kamel El-Deen,Kuniyoshi Shimizu, Suspect and non-target screening of emerging contaminants in wastewater using LC-QTOF/MS: An insight on occurrence, fate, risk, and wastewater management in Asia, Kyushu University Asia Week 2021, 2021.10.|
|16.||Kuniyoshi SHIMIZU, How do we utilize natural resources based on scientific evidence? -Use for pharmaceuticals, functional foods, cosmetics, aromas, and living environment-, International Conference on Natural Sciences, Technology, Islamic Studies, Social Sciences and Humanity (ICoNTISH), 2021.11.|
|17.||清水邦義, How do we utilize natural materials based on scientific evidence? -Use for pharmaceuticals, functional foods, cosmetics, aromas, and living environment-, The 2nd International Conference Series on Biodiversity Status Update of North Borneo, 2021.09.|
|18.||清水邦義, How to utilize natural products as the valuable products -Medicine, Functional Food, aroma, Woody house, Academic Seminar International Conference (2ND ASIC), 2021.08.|
|19.||清水邦義, Lifestyles of Health and Sustainability (LOHAS) concept for the better future: How to utilize natural products as the valuable products -Medicine, Functional Food, aroma,
Woody house, New Challenges in Pharmaceutical Research and Education in Egypt, 2021.03.
|20.||Duanyang Wang, Kuniyoshi Shimizu, Bio-Activity Evaluation of Hibiscus sabdariffa Linn. in Skin Care and Isolation of Contributory Component, Asian Natural Product Conference (Q-AOS), 2021.02.|
|21.||Nguyen Huu Thien, Kuniyoshi Shimizu, From Waste To Medicine: Potential Avocado (Persean americana Mill) Seed Derived Compounds For The Treatment Of Diabetes, Asian Natural Product Conference (Q-AOS), 2021.02.|
|22.||Renandini Danistha, Kuniyoshi Shimizu, LCMS-Based Untargeted Metabolomics of Stipe, Pileus and Spore of Ganoderma lingzhi , Asian Natural Product Conference (Q-AOS), 2021.02.|
|23.||Yanisa Mittraphab, Kuniyoshi Shimizu, Effects of secondary metabolites of Entada phaseoloides leaves on UV radiation damage in human keratinocytes and fibroblasts cell lines, Asian Natural Product Conference (Q-AOS), 2020.10.|
|24.||Jiaxin Fan, Kuniyoshi Shimizu, Liquid chromatography–high-resolution mass spectrometry-based analysis of ceramides in Aspergillus oryzae and Flammulina velutipes, Asian Natural Product Conference (Q-AOS), 2020.10.|
|25.||Yhiya Amen,＠ Ahmed Ashour, Rogers Mwakalukwa, Naomichi Takemoto, Masumi Nagae, ＠Kenshin Hiromatsu, ＠Koichiro Ohnuki, Kuniyoshi Shimizu, Evaluation of the Anti-Hyperglycemic Effect of Pholiota adiposa on Human Subjects and the bio-guided isolation of active components, Asian Natural Product Conference (Q-AOS), 2020.09.|
|26.||＠Satria, Dedi,＠ Sonam Tamrakar, ＠Hiroto Suhara, ＠Shuhei Kaneko, and Kuniyoshi Shimizu., Metabolomics Analysis Revealed Alteration of Lingzhi (Ganoderma lingzhi) metabolites during developmental stages, Asian Natural Product Conference (Q-AOS), 2020.09.|
|27.||Asmaa Kamal El-Deen, Kuniyoshi Shimizu, Application of D-Limonene as a Bio-Based Solvent in Low Density-Dispersive Liquid-Liquid Microextraction of Acidic Drugs from Aqueous Samples, Asian Natural Product Conference (Q-AOS), 2020.05.|
|28.||Taisuke Nakashima, Akiyoshi Honden, Minkai Sun, Yuri Yoshimura, Toshinori Nakagawa, Hiroya Ishikawa, Jun Nagano, Yuki Yamada, Tsuyoshi Okamoto, Yuichiro Watanabe, Shinji Yasunari, Koichiro Ohnuki, Noboru Fujimoto, Kuniyoshi Shimizu. , Effects of Japanese cedar scent on the human brain function, Asian Natural Product Conference (Q-AOS), 2020.05.|
|29.||Enos Tangke Arung, Syafrizal, Swandari Parawita, Yadi, Sukemi, Nataniel Tandirogang, Yhiya Amen, Kuniyoshi Shimizu, Hiroya Ishikawa. , Propolis of Stingless Bees for Skin whitening agent, Asian Natural Product Conference (Q-AOS), 2020.05.|
|30.||Akemi Nishide, Keiko Ohnuki, Tomonari Watanabe, Yoshiteru Nagaregawa, Kuniyoshi Shimizu, Koichiri Ohnuki., Sleep Enhancement by Saffron Extract in Randomized control trial, Asian Natural Product Conference (Q-AOS), 2020.05.|
|31.||Rogers Mwakalukwa, Kuniyoshi Shimizu, Cultivar-specific metabolic profiling of olive leaves cultivars by LCMS based non-targeted metabolomics for the determination of AChE inhibitor metabolites , Asian Natural Product Conference (Q-AOS), 2020.05.|
|32.||Yunfei XIAO，Yhiya AMEN，Asmaa KAMAL EL-DEEN，Naomichi TAKEMOTO，Dongmei WANG，Masako MATSUMOTO，Maki NAGATA，Kenshin HIROMATSU，Kuniyoshi SHIMIZU,
Isolation, Characterization and in Vitro Antioxidant Activity of Polysaccharide from Pholiota adiposa, 第70回日本木材学会, 2020.03, Pholiota adiposa is a species of edible fungus in the family Strophariaceae. It is a valuable mushroom that is grown only in Fukuoka. Polysaccharides extracted from P. adiposa were reported to have immune-stimulatory and antitumor properties, as well as hypolipidemic effect, anti-oxidant and anti-aging activities. We decide to conduct a more systematically studies of P. adiposa Polysaccharide (PAP), including the extraction, isolation, purification, characterization and in vitro antioxidant activity of purified polysaccharide. Crude polysaccharide of P. adiposa was extracted by hot water extraction and alcohol precipitation, and deproteinized by Sevag method. The crude polysaccharide of P. adiposa was further purified using Diethylaminoethanol (DEAE) Sepharose FF and Sephacryl S-200 HR column chromatography. After UV-visible spectrophotometry and gel permeation chromatography (GPC) analysis, the separated polysaccharide was determined to be a purified polysaccharide of 95% and 99% purity. The molecular weight was determined using pullulan standards (molecular weight T-736, 348, 200, 113, 48.8, 23.0, 9.9, 6.6 KD) and the monosaccharide composition was analyzed by HPLC-ELSD. The isolates were analyzed by Fourier Transform Infrared Spectroscopy (FT-IR), Nuclear Magnetic Resonance(NMR) for structural analysis. Then, the antioxidant ability of the polysaccharide fractions was evaluated by the ORAC method and the DPPH method. .
|33.||YHIYA Amen，AHMED Ashour，ROGERS Mwakalukwa，NAOMICHI Takemoto，MASUMI Nagae，KENSHIN Hiromatsu，KOICHIRO Ohnuki，KUNIYOSHI Shimizu, Anti-Hyperglycemic Effect of Pholiota adiposa on Human Subjects and isolation of active components, 第70回日本木材学会, 2020.03, Mushrooms are known to possess bioactive compounds responsible for anti-hyperglycemic effect. An in vitro α-glucosidase inhibition assay of 8 different mushroom species, resulted in the recognition of the methanolic extract of Pholiota adiposa as the strongest inhibitor. A randomized placebo controlled clinical trials performed in 15 healthy Japanese adults, showed significant dose dependent decrease in the blood glucose level, after consumption of 25 g and 50 g fresh equivalent of P. adiposa. Deep investigation of the methanolic extract of P. adipose, guided by an in vitro α-glucosidase inhibitory assay was performed for isolation of the active components. The n-hexane-soluble fraction of the methanolic extract was found active and lipids-like constituents of this fraction were found to be responsible for the activity of the extract. These results will be valuable in establishing P. adiposa as an important source of bioactive compounds capable of suppressing postprandial hyperglycemia in patients with Type 2 diabetes..|
|34.||MWAKALUKWA Rogers，AMEN Yhiya，SHIMIZU Kuniyoshi, Postprandial hyperglycemia lowering effect of metabolites isolated from olive mill wastes (OMW)―An inhibitory activity on α-glucosidase and α-amylase enzymes―, 第70回日本木材学会, 2020.03, Diabetes is characterized by chronic hyperglycemia resulting from either, when the pancreas does not produce enough insulin (type 1), or when the body cannot effectively use the insulin it produces (type 2). The raise of blood glucose level, postprandially, results from hydrolysis of carbohydrates catalyzed by α-glucosidase and α-amylase. Thus, inhibition of these enzymes results into massive reduction of postprandial blood glucose levels ? a major cause of diabetes. In the present study, we report the isolation and anti-diabetic activity (inhibitory effect on α-glucosidase and α-lipase) of eight (8) compounds, one of them being novel, from olive mill wastes (OMW). Additionally, we investigated the enzyme kinetic analysis of the most active compounds to assist the possible characterization of the inhibition mechanisms involved. We observed that four compounds, 1, 2, 4 and 7, had higher activity against both enzymes. The kinetics studies of these compounds revealed that oleanolic acid 1 and luteolin-7-O-glucoside 7 inhibit α-glucosidase in a mixed-type fashion, while 1-acetoxypinoresinol 4 does it uncompetitively. Both mixed type and uncompetitive inhibition are similar to noncompetitive inhibition except that binding of the substrate or the inhibitor affect the enzyme’s binding affinity for the other. Thus for both, mixed type and uncompetitive, Ki value, which measures the affinity of the inhibitor for an enzyme was greater than 1 (Ki >1). Even though the novel compound, 3,4-dihydroxyphenyl-2-ethoxyethanol 5 exhibited poor activity, it helped to study the SAR for compounds of same class (hydroxytyrosol acetate 6 and hydroxytyrosol), whereby we clearly observed that both methoxylation and esterification of different positions does not influence their activity against the enzymes. This work attracts more attention to OMW as a potential source of lead compounds for structural modification to improve and develop new anti-diabetic drugs..|
|35.||Kuniyoshi Shimizu , Development of natural products research from tropical rainforest
How to utilize natural products as the valuable products
-Medicine, Functional Food, Aroma, Woody house-
, World Class Professor Program 2019 , 2019.10.
|36.||Kuniyoshi Shimizu, Lifestyles of Health and Sustainability
(LOHAS) concept for the better future:
How to utilize natural products as the
-Medicine, Functional Food, Aroma,
Woody house-, Natural Product From Tropical Highland Rainforest, 2019.11.
|37.||Kuniyoshi Shimizu, Lifestyles of Helth and Sustinability (LOHAS) concept
for the better future : How to utilize natural products
as the valueable products-Medicine,Functional Food,
Aroma,Woody house, 1st Gunma University Center for Food Science and Wellness (GUCFW) International Symposium and ALCA/JST workshop, 2019.11.
|38.||Kuniyoshi SHIMIZU, Lifestyles of Health and Sustainability (LOHAS) concept for the better future: How to utilize natural products as the valuable products-Medicine, Functional Food, Aroma, Woody house-, The 409th Symposium on Sustainable Humanosphere and the 9th International Symposium for Sustainable Humanosphere, 2019.10.|
|39.||Rogers Mwakalukwa, Ahmed Ashour, Yhiya Amen, Yasuharu Niwa, Sonam Tamrakar, Toshihiro Miyamoto and Kuniyoshi Shimizu , Mast cell stabilizing effect of the isolated compounds from olive mill waste following allergic sensitization - A bioassay-guided fractionation approach, 第26回日本木材学会九州支部大会, 2019.09.|
|40.||Kuniyoshi SHIMIZU,Dedi SATRIA, The importance of triterpenoids for efficient utilization of multi-medicinal mushroom, Ganoderma lingzhi-Analysis of metabolites during developmental stages, The 10th International Medicinal Mushroom Conference, 2019.09.|
|41.||Hiroshima Shota, Kakoi Takumi, Hayashi Junya, Tsutsumi Yuji,Shimizu Kuniyoshi, Cytotoxicity of lignin-derived compounds isolated from bamboo (Phyllostachys pubescence) on cancer cell line, 1st International Lignin Symposium (64th Lignin Symposium), 2019.09.|
|42.||Kuniyoshi SHIMIZU,Dedi SATRIA, The importance of triterpenoids for efficient utilization of multi-medicinal mushroom, Ganoderma lingzhi-Analysis of metabolites during developmental stages-, The 10th International Medicinal Mushroom Conference Third Circular, 2019.09.|
|43.||Kuniyoshi Shimizu, Lifestyles of Health and Sustainability (LOHAS)
concept for the better future: How to utilize natural
products as the valuable products-Medicine,
Functional Food, Aroma, Woody house-, CONFERENCE SCHEDULE OF ICRACOS 2019 “Research and Its Application to Meet The Industrial Era 4.0.“, 2019.09.
|44.||Kuniyoshi SHIMIZU , Lifestyles of Health and Sustainability (LOHAS) for the better future
-Functional Food, Aroma, Woody house- How to utilize natural products, 3rd ICTROPS（The 3rd International Conference on Tropical Studies and its Application）, 2019.08.
|45.||Takashi Kudo,Etsuko Kato,Yuri Yoshimura,Akari Tsunedomi,Le Yang,Akiyoshi Honden,Taisuke Nakashima,Toshinori Nakagawa,Koichiro Ohnuki,Makoto Kawaguchi,Rinpei Sawada,Shinsuke Nishida,Kuniyoshi Shimizu, いぐさがヒトの睡眠に与える影響ーEffects of dried common rush (Juncus effusus) on human sleepー, 第42回日本神経科学大会, 2019.07.|
|46.||Yonathan Asikin,Eriko Haga, Kuniyoshi Shimizu,Miyako Kusano, Hirosuke Oku, Koji Wada, Stress Amelioration Potentials of Shiikuwasha (Citrus Depressa
Hayata) Essential Oil, ACN (Asian Congress of Nutrition) 2019, 2019.08.
|47.||Rogers Mwakalukwa, Asuka Kishikawa, Ahmed Ashour, Yasuharu Niwa, Ahmed Allam and Kuniyoshi Shimizu., Isolation and identification of anti-allergic compounds from Olive Mill Wastes (OMW）, The 68th Annual Meeting of Japan Wood Research Society in Kyoto, 2018.03.|
|48.||Ashour A, Allam A, Amen Y, Shimizu K, Three new Erinacerins from the fruiting bodies of Hericium erinaceums, 2018.03.|
|49.||サトリヤデディ, 中川敏法，清水邦義, Yhiya Amen, Ahmed Ashour, Analysis of Ganoderma lingzhi (Reishi) metabolite during growth stages: a metabolomics approach , The 68th Annual Meeting of Japan Wood Research Society in Kyoto, 2018.03.|
|50.||Nguyen Thi Kim Thoa, Yhiya M. Amen, Kuniyoshi Shimizu, Kenshin Hiromatsu., Isolation and identification of anti-allergic compounds in the fruiting body of Pholiota adiposa mushroom, The 67th annual meeting of Japan Wood Research Society in Fukuoka, 2017.03.|
|51.||Shimizu K, Triterpenoids: Hint for Understanding Multi-Medicinal effects of Ganoderma mushroom, International Mini Symposium of Natural Products Chemistry and Life Sciences in UGSAS-GU, 2016.03.|
|52.||Shimizu K, Tukiran, Andika Pramdya Wardana, Nurul Hidayati, Two phenolic compounds from chloroform extracts of Syzygium polycephalum MIQ. stem bark (Myrtaceae), The 1st International Joint Conference on Science and Technology (IJCST), 2016.10.|
|53.||Shimizu K, Ling Zhi （霊芝）: An amazing multi-medicinal mushroom, T2016 Asian Forum on Modern Research of Traditional Chinese Medicines, 2016.11.|
|54.||Shimizu K, Triterpenoids: Clue for Understanding Multi-Medicinal effects of Ganoderma mushroom, 2016 International Conference on Ganoderma Research, 2016.10.|
|55.||Shimizu Kuniyoshi, Yhiya M. Amen, Qinchang Zhu, Hai-Bang Tran, Satoru Kaifuchi, Mohamed S. Afifi, Ahmed F. Halim, Ahmed Ashour, Amira Mira, New Secondary Metabolites From Ganoderma lingzhi, InternationaSymposium on Natural Products for the future 2016 (ISNPF2016), 2016.09.|
|56.||Shimizu K, Sonam Tamrakar, Hai Bang Tran, Marina Nishida, Satoru Kaifuchi, Hiroto Suhara, Katsumi Doi, Katsuya Fukami, Gopal Prasad Parajuli, Nepalese Wild Mushrooms: A Treasure Trove of Various Biological Activities, 19th International Society for Mushroom Science (ISMS) Congress, Muziekgebouw aan het IJ, 2016.05.|
|57.||Shimizu K, The Multi-functional effects of triterpenpoids isolated from Ganoderma lingzhi, The 55th Aniversary of National Institute of Medicinal Materials (NIMM) conference, 2016.04.|
|58.||Sonam TAMRAKAR, Hai Bang TRAN, Marina NISHIDA, Hiroto SUHARA, Katsumi DOI, Katsuya FUKAMI, Gopal Prasad PARAJULI, Shimizu K, Bioactivity and Chemical Characterization
of some Wild Mushrooms of Nepal, 第66回日本木材学会大会, 2016.03.
|59.||Yhiya Amen, Satoru Kaifuchi, Shimizu K, Methoxyflavones from Ganoderma lingzhi, 第66回日本木材学会大会, 2016.03.|
|60.||Kishikawa A, Yoshinori Katakura, Shimizu K, The Biological Activity of Ethanol Extract of Olive Milled Waste - Anti-allergic effect on RBL-2H3 and Stimulating effect on intracellular concentration of calcium ion in HaCaT, Keystone Symposia: B5: Immunity in Skin Development, Homeostasis and Disease, 2016.03.|
|61.||Shimizu K, Differences in human physiological and psychological response: volatility and humidity conditioning effects caused by different wooden environments
, International Symposium on Frontier Researches in Sustainable Humanosphere 2015, 2015.11.
|62.||Tan H, Shimizu K, Investigation for target protein uf ursolic acid with inhibitory activity on osteoclast differentiation
, ISPSA (Inaugural Symposium of the Phytochemical Society of Asia) 2015, 2015.08.
|63.||Amira M, Wael Alkhiary, Zhu Q, Nakagawa T, Tran H B, Shimizu K, Biotransformation of Isoepoxypteryxin by Cordyceps sineneis through a Novel Microbial pathway and Improved Biological Activities, ISPSA (Inaugural Symposium of the Phytochemical Society of Asia) 2015, 2015.08.|
|64.||Mira A, Elshebiny N, Shebl A, Tran HB, In vivo and In vitro Hepatoprotective Activities of The Methanol Extract of Angelica shikokiana and Its Major Coumarin: Isoepoxypteryxin Against Hepacocellular Carcinoma
, ISPSA (Inaugural Symposium of the Phytochemical Society of Asia) 2015, 2015.08.
|65.||Shimizu K, Multi-functional effects of triterpenoids isolated from Ganoderma mushroom
, The First Chinese Lingzhi Conference, 2015.08.
|66.||Shimizu K, Differences in physiological and psychological response to human,volatile and humidity conditioning effect caused by different wooden environment
, Integration of Psychology, Physiology, Education, and Policy For the Forest Healing, 2015.07.
|67.||Amira Mira, Wael Alkhiary, Kuniyoshi Shimizu, Anticoagulant active compounds of Angelica shikokiana, 第65回日本木材学会, 2015.03.|
|68.||Sonam Tamrakar, Marina Nishida, Hiroto Suhara, Investigation of the Antioxidant Activities of the Wild Mushrooms of Nepal, 第６５回日本木材学会, 2015.03.|
|69.||S. Matsumoto, T. Okamoto, A. Yamamoto, K. Shimizu, Restorative effect of wooden materials on electrophysiological responses in humans, Society for Neuroscience 2013, 2013.11.|
|70.||K. Shimizu (Keynote speaker), Molecular target of triterpenoid with anticancer activity isolated from medicinal mushroom, Ganoderma lingzhi, The Fifth International Symposium of Indonesian Wood Research Society, 2013.11.|
|71.||A. Ashour, S. EI Sharkawy, M. Amer, K. Shimizu, Synthesis and anti-proliferative activity of some oleanolic acid derivatives as potent topoisomerase inhibitors, The Fifth International Symposium of Indonesian Wood Research Society, 2013.11.|
|72.||K. Shimizu, Utilization of Biomass (wood, mushroom etc) by Focusing on Their Biological Activities, EUIJ-Kyushu 3rd Annual International Conference, 2013.11.|
|73.||Fatmawatia S, Shimizu K, Ersam T, Aldose reductase inhibitory effect of Garcinia mangostana constituents, 第20回日本木材学会九州支部大会, 2013.09.|
|74.||Tan H, Shimizu K, Osteoclast Differentiation Inhibitors from Loquat Leaves (Eriobotrya japonica): Structure Activity Relationship, 第20回日本木材学会九州支部大会, 2013.09.|
|75.||Ashoura A, El-Sharkawy S, Amer M, Marzouk A, Kondo R, Shimizu K, Corncobs as a Potential Source of Functional Chemicals, 第20回日本木材学会九州支部大会, 2013.09.|
|76.||清水 邦義, Utilization of forest bio-resources (wood, mushroom etc) focusing on their biological activities, JBEI (Joint BioEnergy Institute), 2012.08.|
|77.||K. Shimizu, T. Hui, The Inhibitory Effect of Loquat Leaves (Eriobotrya japonica) on Ovariectomy-induced Bone Loss and Osteoclast Differentiation, 第19回日本木材学会九州支部大会 2012年8月24-25 宮崎市（口頭発表）, 2012.08.|
|78.||Iwasa K, Tan H, Midorikawa S, Nemoto M, Mizushima A, Yoshikawa K, Shimizu K, Maruyama K, Effect of compound X for working memory performance and amyloid-β protein in mice, 第87回日本薬理学会.|