Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Yamauchi Nobuhiko Last modified date:2024.06.03

Associate Professor / Department of Animal and Marine Bioresource Sciences / Department of Bioresource Sciences / Faculty of Agriculture


Papers
1. Yun CS, Saito Y, Rahman AMI, Suzuki T, Takahashi H, Kizaki K, Khandoker MAMY, Yamauchi N., C-C motif chemokine ligand 2 regulates prostaglandin synthesis and embryo attachment of the bovine endometrium during implantation., Cell Tissue Res. 2024 May;396(2):231-243. doi: 10.1007/s00441-024-03869-8. Epub 2024 Mar 5. PMID: 38438567, 2024.05.
2. Nishino D, Kotake A, Yun CS, Rahman AMI, El-Sharawy M, Yamanaka KI, Khandoker MAMY, Yamauchi N., Gene expression of bovine endometrial epithelial cells cultured in matrigel.
, Cell Tissue Res. 2021 Apr 10. doi: 10.1007/s00441-021-03418-7. Online ahead of print., 2021.04, Glandular epithelial cells (GE) in the endometrium are thought to support the elongation and survival of ruminant embryos by secreting histotrophs. In the present study, the gene expression of bovine endometrial epithelial cells cultured in matrigel was analyzed and examined whether it could be an in vitro model of GE. Bovine endometrial epithelial cells (BEE) and stromal cells (BES) were isolated from the slaughterhouse uteri and cultured in DMEM/F12 + 10% FBS. BEE showed the gland-like structure morphological changes when cultured in 15% matrigel but could not be identified in higher concentrations of the matrigel (30% or 60%). The expression of typical genes expressed in GE, SERPINA14 and GRP, was substantially high in matrigel-cultured BEE than in monolayer (P
3. Yun CS, Masaka H, Nishino D, Horaku S, Rahman AMI, Khandoker MAMY, Yamauchi N., Analysis of novel embryonic factors of cattle and effects on endometrial cells in vitro., Anim Reprod Sci. 2021 Mar;226:106696. doi: 10.1016/j.anireprosci.2021.106696., 226, 2021.03, Interferon tau (IFNT) is thought to have essential functions in maternal recognition and establishment of pregnancy in ruminants. There, however, is a lack of research on embryonic factors that affect pregnancy other than IFNT. The present study was conducted to determine what are other embryo-derived factors involved in pregnancy recognition and to identify effects on endometrial cells using an in vitro culture system. With use of LC-MS/MS procedures to evaluate the supernatant of elongating embryos of cattle in culture, there was detection of 78 secretary proteins including five cytokines and two growth factors. Then there was analysis for up-regulated genes using ingenuity pathway procedure, IFNT and MIF were identified as upstream regulators of 37 and five genes, respectively. The mRNA transcript of MIF receptors was identified in endometrial cells, however, not in embryos. Among genes induced by MIF, CCL2, IL7 and IL23A transcripts were identified in endometrial cells. When endometrial cells were treated with interferon alpha (IFNA) and MIF, the CCL2 transcript was in a larger abundance of endometrial epithelial and polymorphonuclear neutrophil cells, and there was a larger abundance of there mRNA transcripts as a result of MIF treatment of peripheral blood mononuclear cells. In conclusion, MIF secreted by elongating embryos of cattle synergistically regulates relative abundances of specific mRNA transcripts of endometrial cells when there is treatment with IFNA, indicating further there are several factors other than IFNT that have effects on gene expression in the endometrium during early stages of gestation in cattle..
4. Rahman ANMI, Yamashita S, Fujihara T, Islam MR, Fujihara T, Yamaguchi H, Kawahara M, Takahashi M, Takahashi H, Gotoh T, Yamauchi N., Type-I interferon regulates matrix metalloproteinases clearance of the bovine endometrial spheroid, Anim Sci J. Jan-Dec 2020;91(1):e13350. doi: 10.1111/asj.13350., 89, 1609-1621, 2020.01, This study investigated the effect of type-I interferon (IFN) on the expression of matrix metalloproteinases (MMPs) of the bovine endometrial stromal cells (BES) and epithelial cells (BEE). The cells were separated and purified from the caruncles and cultured in DMEM/F-12 containing 10% fetal bovine serum. Spheroids were generated by using ascorbate. Zymograms of the supernatant showed that BEE
predominantly expressed MMP-9, whereas MMP-2 was expressed in BES and homospheroids. While MMPs expression was not detected in hetero-spheroids. Real-time quantitative PCR revealed that type-I IFN and P4 suppressed the gene expression of MMP-2 and MMP-9 in hetero-spheroids, respectively. On the other hand, gelatin zymography analysis of the supernatant showed that type-I IFN strongly promote the clearance of MMPs. While zymograms of the MMPs stocked in the hetero-spheroids were significantly reduced by type-I IFN. Phenylmethanesulfonyl fluoride and leupeptin (both are serine proteinase inhibitors) significantly repressed the clearance of MMP-2 and MMP-9 induced by type-I IFN. Moreover, collagen fibers in heterospheroids significantly decreased after the treatment with type-I IFN. In conclusion, it was suggested that type-I IFN participate in the tissue remodeling by regulation the clearance of MMPs..
5. Musavi SAA, Yamashita S, Fujihara T, Masaka H, Islam MR, Kim S, Gotoh T, Kawahara M, Tashiro K, Yamauchi N., Analysis of differentially expressed genes and the promoters in bovine endometrium throughout estrus cycle and early pregnancy., Anim Sci J. 2018 Nov;89(11):1609-1621. doi: 10.1111/asj.13091. Epub 2018 Sep 5., 2018 Nov;89(11):1609-1621. doi: 10.1111/asj.13091. Epub 2018 Sep 5., 89, 1609-1621, 2018.11, Endometrial gene expression is primarily regulated by the ovarian steroids and pregnancy recognition factors. The present study was aimed to characterize differential expression genes (DEGs) in bovine endometrium together with the analysis of their promoter region. Bovine uteri at follicular stage (FS), luteal stage (LS) and implantation stage (IS) at day18 of pregnancy were collected. Total RNA extracted and prepared cDNA were then subjected to high-throughput sequencing. For promoter analysis, 1kb upstream promoter region of each DEG was analyzed. The number of highly expressed DEGs were 496 and 597 at FS and LS, respectively. When compared the gene expression of IS with LS, 383 and 346 DEGs showed higher and lower expression at IS, respectively. It was also observed that 20-30 transcription factors (TFs) were included in each DEGs. Additionally, promoter analyses estimated 150-160 TFs for each stage. DLX4 and IRF4 at FS, and IRF5, IRF9, STAT1 and STAT2 at IS were in common to DEGs and estimated TFs, respectively. The present study highlighted potential molecular mechanisms controlling endometrial function during estrus cycle and implantation stage, which will further guide to better understand the endometrial functions in future studies..
6. Tae Young KIL, Han Su RYU and Nobuhiko YAMAUCHI , The Effect of Integrated Play Therapy on Self-Expression and Depression in Elderly Women Living Alone in Rural Areas, J Faculty Agriculture, Kyushu University., 63, 2, 265-270, 2018.10, The aim of this study is to investigate the effects of an integrated play therapy program on self-expression, depression, and psychosocial well-being among elderly women who live alone in rural areas. The study was conducted on a group of elderly women, aged 65 and over, who were living alone. Researchers selected one village in the township of A-country, Chungcheongnam-do, South Korea. Data were collected from 24 experimental groups and 24 non-participating control groups. The program was applied to the experimental group for a total of eight weeks (one two-hour session per week). An analysis compared the results of the non-identical control group before and after the program; the effect of the program was measured by assessing participants using a follow-up test, before, immediately after, and ten weeks after the program. The results showed that levels of self-expression increased significantly in the experimental group, from 2.96 before the program, to 3.45 immediately after the program, and 4.13 ten weeks later. Rates of depression decreased significantly in the follow-up tests, measuring 0.85, 0.67, and 0.21 before, after, and ten weeks later. These results show that the play therapy program was effective and had a continuous effect on elderly participants. This study confirms that integrated play therapy is an effective program for developing self-expression and reducing depression among elderly women who live alone in rural areas. Based on these results, this article discusses the impact of the program on self-expression and depression among elderly women who live alone in rural areas; it proposes a policy as a basis for operating and using integrated play therapy to help vulnerable elderly people..
7. Ibrahim EL-SHAMAA, Mostafa IBRAHIM, Mohamed EL-SHARAWY, Raed MAHMOUD, Kaiyu KUBOTA, Nobuhiku YAMAUCHI and El-Shenawy El-SIEFY, Effect of Various Coconut (Cocos nucifera) Milk Concentrations Instead of Egg Yolk on Freezability and Fertility of Frozen Holstein Bull Spermatozoa., J Faculty Agriculture, Kyushu University., 63, 2, 265-270, 2018.10, The study was carried out to investigate if the substitution of egg yolk (EY) with various coconut (Cocos nucifera) milk concentrations in extenders can improve the freezability and fertility of frozen Holstein bull spermatozoa. Semen from five Holstein bulls was collected twice weekly and ejaculates with 75% progressive motility and more 85% normal sperm morphology prior to cryopreservation were pooled in order to have sufficient semen for a replicate and to eliminate the bull effect. Five extenders were used. Tris 20% egg yolk extender with 7% glycerol as a control, and substitution of whole egg yolk with 5, 10, 15, and 20% coconut milk. Semen was diluted to 80 million sperm/ml packaged into 0.25ml straws, cooled held at 5ºC for 4 h, and then frozen in liquid nitrogen and stored at –196ºC until for artificial insemination. Sperm progressive motility, live sperm, sperm abnormality, intact sperm acrosome and plasma membrane integrity were evaluated post dilution, post–equilibration and post frozen–thawed processes. The results revealed that 5% coconut milk extender was more effective in preservation of progressive motility, live sperm, sperm abnormality, intact sperm acrosome and plasma membrane integrity of Holstein spermatozoa than whole egg yolk extender and other coconut milk extenders. Fertility rates were higher in 5% and 10% coconut milk extenders compared with whole egg yolk extender (65% and 55% vs 45%, respectively) and other 15% and 20% coconut milk extenders (45% and 40%, respectively). It was concluded that 5% coconut milk extender improved the freezability and fertility of Holstein bull spermatozoa..
8. Park KS, Chun JL, Kim EY, Lee JH, Lee HS, Yamauchi N, Lee KB, Kim MK., Protective effect of Trehalose on Canine Spermatozoa in Cryopreservation., J Faculty Agriculture, Kyushu University., 63, 1, 53-60, 2018.04.
9. Zhuang L, Chun JL, Wu J, Lee JH, Kim EY, Lee BM, Yamauchi N, Lee HS, Kim MK. , Production of Female Bovine Embryos with Sperm Sorted by Magnetic Activated Cell Sorting., J Faculty Agriculture, Kyushu University., 63, 1, 45-52, 2018.04.
10. Yun CS, Chun JL,Yu J, Kim DE, Park KS, Kim EY, Lee JH, Yoon KB, Yamauchi N, Lee S, Kim MK., Genetic Structure Analysis of Northeast Asian Sika Deer (Cervus nippon) Populations using Mitochondrial and Microsatellite Markers., J Faculty Agriculture, Kyushu University., 63, 1, 37-44, 2018.04.
11. El-behnsawy B, El-shenawy ES, El-halawany R, El-sharawy M, Kubota K, Yamauchi N, El-Salam AM., Effect of Glutathione on Cryopreservation of Buck Spermatozoa., J Faculty Agriculture, Kyushu University. , 62, 2, 367-372, 2017.10.
12. Soltan W, El-shenawy ES, El-razek IABD, El-sharawy M, Kubota K, Yamauchi N, El-shamaa I., Soybean Milk-Based Extender for Cryopreservation of Buck Spermatozoa., J Faculty Agriculture, Kyushu University. 2017;62(2):361-366., 62, 2, 361-366, 2017.10.
13. Islam MR, Ikeguchi Y, Yamagami K, El-Sharawy M, Yamauchi N., Development of an in vitro model to study uterine functions and early implantation using rat uterine explants., Cell Tissue Res., 370, 501-512, 2017.06, The study was conducted to develop an in vitro model using rat uterine explants to explore complex uterine functions. Rat uterine explants (1-2 mm) were isolated, cultured and further characterized. Cultured explants after steroid hormone treatment exposed that both Muc1 and Pr are up-regulated significantly (P
14. El-sharawy M, Eid E, Darwish S, El-razek IABD, Islam MR, Kubota K, Nobuhiko Yamauchi, El-shamaa I, Effect of Organic and Inorganic Selenium Supplementation on Semen Quality and Blood Enzymes in Buffalo Bulls., Anim Sci J., 21, 2016.05.
15. Akizawa H, Nagatomo H, Odagiri H, Kohri N, Nobuhiko Yamauchi, Yanagawa Y, Nagano M, Takahashi M, Kawahara M, Conserved roles of fibroblast growth factor receptor 2 signaling in the regulation of inner cell mass development in bovine blastocysts.
, Mol Reprod Dev. , 2016.04.
16. Md. Rashedul Islam, Yamagami K, Yashii Y, Nobuhiko Yamauchi, Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells in vitro.
, J Reprod Dev, 2016.03.
17. Egashira A, Nobuhiko Yamauchi, Md. Rashedul Islam, Yamagami K, Tanaka A, Suyama H, El-Sayed EM, Shoji Tabata, Kuramoto T, Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development., Anim Sci J., 2016.02.
18. Zhao L, Isayama K, Chen H, Nobuhiko Yamauchi, Shigeyoshi Y, Hashimoto S, Masa-aki Hattori, The nuclear receptor REV-ERBα represses the transcription of growth/differentiation factor 10 and 15 genes in rat endometrium stromal cells., Physiol Rep. , 2016.02.
19. Nagatomo H, Kohri N, Akizawa H, Hoshino Y, Nobuhiko Yamauchi, Kono T, Takahashi M, Kawahara M, Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development., Anim Sci J. , 87, 3, 457-461, 2015.12.
20. Yamagami K, Md. Rashedul Islam, Yoshii Y, Mori K, KOSUKE TASHIRO, Nobuhiko Yamauchi, Preimplantation embryo-secreted factors modulate maternal gene expression in rat uterus., Cell Tissue Res. , 364, 453-463, 2015.12.
21. Nagatomo H, Akizawa H, Sada A, Kishi Y, Yamanaka K, Takuma T, Sasaki K, Nobuhiko Yamauchi, Yanagawa Y, Nagano M, Kono T, Takahashi M, Kawahara M, Comparing spatial expression dynamics of bovine blastocyst under three different procedures: in-vivo, in-vitro derived, and somatic cell nuclear transfer embryos.
, Jpn J Vet Res. 2015 Nov;63(4):159-71., 63, 4, 159-171, 2015.11.
22. Shirozu T, Sasaki K, Kawahara M, Yanagawa Y, Nagano M, Nobuhiko Yamauchi, Takahashi M, Expression dynamics of bovine MX genes in the endometrium and placenta during early to mid pregnancy., J Reprod Dev, 62, 1, 29-35, 2015.10.
23. Egashira A, Nobuhiko Yamauchi, Tanaka K, Mine C, Otsubo H, Murakami M, Md. Rashedul Islam, Ohtsuka M, Yoshioka N, Kuramoto T, Developmental capacity and implantation potential of the embryos with multinucleated blastomeres., J Reprod Dev, 61, 6, 595-600, 2015.09.
24. Chen H, Isayama K, Kumazawa M, Zhao L, Nobuhiko Yamauchi, Yasufumi Shigeyoshi, Seiichi Hashimoto, Masa-aki Hattori, Integration of the nuclear receptor REV-ERBα linked with circadian oscillators in the expressions of Alas1, Ppargc1a, and Il6 genes in rat granulosa cells., Chronobiol Int. 2015; 32(6):739-49., 32, 6, 739-749, 2015.06.
25. Isayama K, Zhao L, Chen H, Nobuhiko Yamauchi, Shigeyoshi Y, Hashimoto S, Masa-aki Hattori, Removal of Rev-erbα inhibition contributes to the prostaglandin G/H synthase 2 expression in rat endometrial stromal cells., Am J Physiol Endocrinol Metab. 2015 Apr 15;308(8):E650-61., 2015.04.
26. Tasaki H, Zhao L, Isayama K, Chen H, Nobuhiko Yamauchi, Shigeyoshi Y, Hashimoto S, Masa-aki Hattori, Inhibitory role of REV-ERBα in the expression of bone morphogenetic protein gene family in rat uterus endometrium stromal cells., Am J Physiol Cell Physiol. 2015 Apr 1;308(7):C528-38., 2015.04.
27. Yamauchi K, Nobuhiko Yamauchi, Yamagami K, Nakamura N, Yamashita S, Islam MR, Shoji Tabata, Yahiro K, Tamura T, Hashizume K, Masa-aki Hattori, Development of an in vitro model for the analysis of bovine endometrium using simple techniques., Anim Sci J. 2015 May;86(5):523-31., 2015.05.
28. Yamagami K, Nobuhiko Yamauchi, Kubota K, Nishimura S, Vishwajit Sur Chowdhury, Yamanaka K, Takahashi M, Shoji Tabata, Masa-aki Hattori, Expression and Regulation of Foxa2 in the Rat Uterus during Early Pregnancy., J Reprod Dev, 2014.09.
29. Isayama K, Chen H, Nobuhiko Yamauchi, Masa-aki Hattori, REV-ERBα Inhibits the PTGS2 Expression in Bovine Uterus Endometrium Stromal and Epithelial Cells Exposed to Ovarian Steroids., J Reprod Dev, 2014.07.
30. Kito G, Tanaka H, Tomoki   Soh, Nobuhiko Yamauchi, Masa-aki Hattori, The Putative Promoters of Germ Cell-specific Genes and Nanog are Hypomethylated in Chicken Sperm.
, J Reprod Dev, 2014.03.
31. Mori M, Kuwano T, Kamori T, Isozaki Y, Nishihara T, Nobuhiko Yamauchi, Masa-aki Hattori, Effect of ATP-binding cassette subfamily B member 1 on bovine blastocyst implantation., Theriogenology, 2014.03.
32. Kawamura M, Tasaki H, Misawa I, Chu G, Nobuhiko Yamauchi, Masa-aki Hattori, Contribution of testosterone to the clock system in rat prostate mesenchyme cells., Andrology, 2014.03.
33. Tasaki H, Zhao L, Isayama K, Chen H, Nobuhiko Yamauchi, Shigeyoshi Y, Hashimoto S, Masa-aki Hattori, Profiling of circadian genes expressed in the uterus endometrial stromal cells of pregnant rats as revealed by DNA microarray coupled with RNA interference. , Frontiers in Endocrinology — Systems and Translational Endocrinology (Circadian Clock and Pregnancy) 4: 82 (2013)., 2013.07.
34. Chen H, Zhao L, Kumazawa M, Nobuhiko Yamauchi, Shigeyoshi Y, Hashimoto S, Masa-aki Hattori, Down-regulation of core clock gene Bmal1 attenuates expression of progesterone and prostaglandin biosynthesis-related genes in rat luteinizing granulosa cells., Am J Physiol Cell Physiol. 2013 Apr 17., 2013.04.
35. Nishimura K, Nakano N, Vishwajit Sur Chowdhury, Kaneto M, Torii M, Nobuhiko Yamauchi, Masa-aki Hattori, Kawai M, Effect of PPARβ/δ Agonist on the Placentation and Embryo-Fetal Development in Rats., Birth Defects Res B Dev Reprod Toxicol. 2013 Apr;98(2):164-9., 2013.04.
36. Chen H, Zhao L, Chu G, Kito G, Nobuhiko Yamauchi, Shigeyoshi Y, Hashimoto S, Masa-aki Hattori, FSH induces the development of circadian clockwork in rat granulosa cells via a gap junction protein Cx43-dependent pathway., Am J Physiol Endocrinol Metab. 2013 Mar 15;304(6):E566-75., 2013.03.
37. Mori M, Kasa S, Isozaki Y, Kamori T, Yamaguchi S, Ueda S, Kuwano T, Eguchi M, Isayama K, Shotaro Nishimura, Shoji Tabata, Nobuhiko Yamauchi, Masa-aki Hattori, Improvement of the cellular quality of cryopreserved bovine blastocysts accompanied by enhancement of the ATP-binding cassette sub-family B member 1 expression., Reprod Toxicol. 2013 Jan;35:17-24., 2013.01.
38. Chen H, Chu G, Zhao L, Yamauchi N, Shigeyoshi Y, Hashimoto S, Hattori MA., Rev-erbα regulates circadian rhythms and StAR expression in rat granulosa cells as identified by the agonist GSK4112., Biochem Biophys Res Commun. 2012 Apr 6;420(2):374-9. , 2012.04.
39. Chu G, Misawa I, Chen H, Yamauchi N, Shigeyoshi Y, Hashimoto S, Hattori MA., Contribution of FSH and triiodothyronine to the development of circadian clocks during granulosa cell maturation., Am J Physiol Endocrinol Metab. 2012 Mar;302(6):E645-53. , 2012.03.
40. Chu G, Yoshida K, Narahara S, Uchikawa M, Kawamura M, Yamauchi N, Xi Y, Shigeyoshi Y, Hashimoto S, Hattori MA., Alterations of circadian clockworks during differentiation and apoptosis of rat ovarian cells., Chronobiol Int. 2011 Jul;28(6):477-87., 2011.07.
41. Nishimura K, Yamauchi N, Chowdhury VS, Torii M, Hattori MA, Kaneto M., Expression of peroxisome proliferator-activated receptor isoforms in the rat uterus during early pregnancy., Cell Tissue Res. 2011 Aug;345(2):275-84., 2011.07.
42. Uchikawa M, Kawamura M, Yamauchi N, Hattori MA., Down-regulation of circadian clock gene period 2 in uterine endometrial stromal cells of pregnant rats during decidualization., Chronobiol Int. 2011 Feb;28(1):1-9., 2011.02.
43. Yokota K, Hirano T, Urata N, Yamauchi N, Hattori MA., Upregulation of P-Glycoprotein Activity in Porcine Oocytes and Granulosa Cells During In Vitro Maturation., J Reprod Dev. 2011 Jan 28. , 2011.01.
44. Kito G, Aramaki S, Tanaka K, Soh T, Yamauchi N, Hattori MA., Temporal and Spatial Differential Expression of Chicken Germline-specific Proteins cDAZL, CDH and CVH During Gametogenesis., J Reprod Dev. 2010 Mar 24. , 2010.03.
45. Kubota K, Yamauchi N, Yamagami K, Nishimura S, Gobaru T, Yamanaka K, Wood C, Soh T, Takahashi M, Hattori MA., Steroidal regulation of Ihh and Gli1 expression in the rat uterus., Cell Tissue Res. 2010 May;340(2):389-95. Epub 2010 Mar 16., 2010.03.
46. Yoshida K, He PJ, Yamauchi N, Hashimoto S, Hattori MA., Up-regulation of circadian clock gene Period 2 in the prostate mesenchymal cells during flutamide-induced apoptosis., Mol Cell Biochem. 2010 Feb;335(1-2):37-45. Epub 2009 Aug 28., 2010.02.
47. Kurokawa M, Sato F, Aramaki S, Soh T, Yamauchi N, Hattori MA., Monitor of the myostatin autocrine action during differentiation of embryonic chicken myoblasts into myotubes: effect of IGF-I., Mol Cell Biochem. 2009 Nov;331(1-2):193-9. Epub 2009 May 24., 2009.11.
48. Aramaki S, Kubota K, Soh T, Yamauchi N, Hattori MA., Chicken Dead End Homologue Protein is a Nucleoprotein of Germ Cells Including Primordial Germ Cells., J Reprod Dev. 2009 Feb 5., 2009.02.
49. Hirata M, He PJ, Shibuya N, Uchikawa M, Yamauchi N, Hashimoto S, Hattori MA., Progesterone, but not estradiol, synchronizes circadian oscillator in the uterus endometrial stromal cells., Mol Cell Biochem. 2009 Apr;324(1-2):31-8. Epub 2008 Dec 20., 2008.12.
50. Matsumoto K, Yamauchi N, Watanabe R, Oozono S, Kubota K, Nishimura K, Wood C, Soh T, Kizaki K, Hattori MA., In vitro decidualization of rat endometrial stromal cells., Cell Tissue Res. 2009 Mar;335(3):575-83. Epub 2008 Dec 17., 2008.12.
51. Oozono S, Yamauchi N, Nishimura K, Matsumoto K, Watanabe R, Kubota K, Aramaki S, Sato F, Wood C, Soh T, Kizaki K, Hattori MA., Expression of rat uterine serine proteinases homologous to mouse implantation serine proteinase 2., J Exp Zoolog B Mol Dev Evol. 2008 Dec 15;310(8):642-9., 2008.12.
52. Kubota K, Yamauchi N, Matsumoto K, Watanabe R, Oozono S, Aramaki S, Wood C, Soh T, Hattori MA., Expression of hedgehog family genes in the rat uterus during early pregnancy., J Reprod Dev. 2008 Oct;54(5):340-5. Epub 2008 Jul 8., 2008.07.
53. Gamo T, Yamauchi N, Nishimura K, Watanabe R, Matsumoto K, Oozono S, Kubota K, He PJ, Soh T, Hattori MA., Effects of tumor necrosis factor-alpha on cell proliferation, prostaglandins and matrix-metalloproteinases production in rat endometrial stromal cells cultured in vitro, J Exp Zool Part A Ecol Genet Physiol, 2007.12.
54. Kubota K, Sato F, Aramaki S, Soh T, Yamauchi N, Hattori M A, Ubiquitous expression of myostatin in chicken embryonic tissues: its high expression in testis and ovary, Comp Biochem Physiol A Mol Integr Physiol, 2007.11.
55. He PJ, Hirata M, Yamauchi N, Hattori MA., Up-regulation of Per1 expression by estradiol and progesterone in the rat uterus, J Endocrinol, 2007.09.
56. He PJ, Hirata M, Yamauchi N, Hashimoto S, Hattori MA., Gonadotropic regulation of circadian clockwork in rat granulosa cells., Mol Cell Biochem, 2007.08.
57. Yamada K, Shibuya N, Aramaki S, Okuno S, Soh T, Yamauchi N, Hattori MA., Development of multidrug resistance type I Cmdr1 expression in chicken embryonic gonads., Comp Biochem Physiol A Mol Integr Physiol, 2007.08.
58. He PJ, Hirata M, Yamauchi N, Hashimoto S, Hattori MA., The disruption of circadian clockwork in differentiating cells from rat reproductive tissues as identified by in vitro real-time monitoring system, J Endocrinol, 2007.06.
59. Fukuda H, He PJ, Yokota K, Soh T, Yamauchi N, Hattori MA., Progesterone-dependent and -independent expression of the multidrug resistance type I gene in porcine granulosa cells., Mol Cell Biochem, Mol Cell Biochem. 2007 Apr;298(1-2):179-86., 2007.04.
60. He PJ, Fujimoto Y, Yamauchi N, Hattori MA. , Real-time monitoring of cAMP response element binding protein signaling in porcine granulosa cells modulated by ovarian factors., Mol Cell Biochem, Mol Cell Biochem. 2006 Oct;290(1-2):177-84. Epub 2006 Apr 22., 2006.10.
61. Sato F, Kurokawa M, Yamauchi N, Hattori MA. , Gene silencing of myostatin in differentiation of chicken embryonic myoblasts by small interfering RNA., Am J Physiol Cell Physiol, Am J Physiol Cell Physiol. 2006 Sep;291(3):C538-45. Epub 2006 Apr 12. , 2006.09.
62. Fukuda H, Arai M, Soh T, Yamauchi N, Hattori MA. , Progesterone regulation of the expression and function of multidrug resistance type I in porcine granulosa cells., Reprod Toxicol, Reprod Toxicol. 2006 Jul;22(1):62-8. Epub 2005 Dec 6. , 2006.07.
63. Arai M, Yamauchi N, Fukuda H, Soh T, Hattori MA. , Development of multidrug resistance type I P-glycoprotein function during in vitro maturation of porcine oocyte., Reprod Toxicol, Reprod Toxicol. 2006 Jan;21(1):34-41. Epub 2005 Jun 29. , 2006.01.
64. Aramaki S, Sato F, Soh T, Yamauchi N, Sakai T, Hattori MA. , Temporal and spatial expression of TGF-beta2 in chicken somites during early embryonic development., J Exp Zoolog A Comp Exp Biol, 10.1002/jez.a.148, 303A, 5, 323-330, J Exp Zoolog A Comp Exp Biol. 2005 May 1;303(5):323-30. , 2005.05.
65. Aramaki S, Sato F, Soh T, Yamauchi N, Hattori M-A., Differential expression sites of TGF-βisoforms in chicken leg buds during morphogenesis., Canadian J Zool. 2005;83:620-625., 83, 620-625, J Reprod Dev. 2004 Oct;50(5):599-60, 2005.10.
66. Hirano T, Yamauchi N, Sato F, Soh T, Hattori MA. , Evaluation of RNA interference in developing porcine granulosa cells using fluorescence reporter genes., J Reprod Dev, 10.1262/jrd.50.599, 50, 5, 599-603, J Reprod Dev. 2004 Oct;50(5):599-60, 2004.10.
67. N Yamauchi, T Takezawa, K Kizaki, CB Herath, K Hashizume, Proliferative potential of endometrial stromal cells, and endometrial
and placental expression of cyclin in bovine., J Reprod Dev, 10.1262/jrd.49.553, 49, 6, 553-560, Vol.49(2003), 553-560., 2003.10.
68. Hirao Y, Nishimoto N, Kure_bayashi S, Takenouchi N, Yamauchi N, Masuda H, Nagai T, Influence of meiotic inhibition by butyrolactone-I during germinal vesicle stage on the ability of porcine oocytes to be activated by electric stimulation after nuclear maturation., Zygote, Vol.11(2003), 191-7., 2003.08.
69. Suzuki K, Geshi M, Yamauchi N, Nagai T, Functional changes and motility characteristics of Japanese Black bull spermatozoa separated by Percoll., Anim Reprod Sci, Vol.77(2003), 157-72., 2003.07.
70. N Yamauchi, K Kizaki, O Yamada, T Takahashi, CB Herath, K Hashizume, Expression of integrin subunits depend on bovine endometrial stromal cells cultured in vitro., Connective Tissue, Vol.35(2003), 1-7., 2003.02.
71. N Yamauchi, O Yamada, T Takahashi, K Imai, T Sato, A Ito, K Hashizume, A three-dimensional cell culture model for bovine endometrium; regeneration of a multicellular spheroid using ascoebate., Placenta, Vol.24(2002), 258-269., 2002.10.
72. K Kizaki, O Yamada, H Nakano, T Takahashi, N Yamauchi, K Imai, K Hashizume, Cloning and localization of heparanase in bovine placenta., Placenta, Vol.24(2002), 424-430., 2002.10.
73. M Sakaguchi, T Dominko, N Yamauchi, ML Leibfried-Rutledge, T Nagai, N First, Possible mechanism for acceleration of meiotic progression of bovine follicular oocytes by growth factors in vitro., Reproduction, Vol.123(2002), 135-142., 2001.09.
74. N Yamauchi, O Yamada, T Takahashi, K Hashizume, Spheroid formation of bovine endometrial stromal cells using non-
adherent culture plate., Journal of Reproduction and Development, Vol.47 (2001), 165-171., 2001.04.
75. T Nagai, N Yamauchi, K Kikuchi, Nuclear and cytoplasmic maturation in vitro of porcine oocytes., Journal of Reproduction and Development, Vol.47 (2001), S55-S62., 2001.04.
76. M Geshi, N Takenouchi, N Yamauchi, T Nagai, Effects of sodium pyruvate in nonserum maturation medium on maturation, fertilization, and subsequent development of bovine oocytes with or without cumulus cells., Biology of Reproduction, Vol.63(2000), 1730-1734., 2000.07.
77. N Yamauchi, T Nagai, Male pronucleus formation in denuded porine oocytes after in vitro maturation in the presence of cysteamine., Biology of Reproduction, Vol.61, p823-833., 1999.04.
78. K Kikuchi, T Nagai, J Ding, N Yamauchi, J Noguchi, Y Izaike, Cytoplasmic maturation for oocyte activation of porcine follicular oocytes cultured and arrested at Metaphase-I., Journal of Reproduction and Fertility, Vol.116, p143-156., 1999.01.
79. N Yamauchi, H Sasada, E Soloy, T Dominko, K Kikuchi, T Nagai, Effect of hormones and osmolarity in the culture medium on germinal vesicle breakdown of porcine oocytes., Theriogenology, Vol.52, p153-162., 1998.11.
80. N Yamauchi, H Sasada, S Sugawara., In vitro maturation of porcine follicular oocytes using Medium-II. , Tohoku Journal of Agricultural Research, 45, 55-60, 1995.04.
81. N Yamauchi, H Sasada, S Sugawara, T Nagai., Effect of culture conditions on artifitial activation of porcine oocytes matured in vitro., Reprod Fertil Dev., 8, 1153-1156, 1996.01.