Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Yohei Shimasaki Last modified date:2024.06.27

Associate Professor / Animal and Marine Bioresource Sciences / Department of Bioresource Sciences / Faculty of Agriculture


Papers
1. Takai Yuki, Tominaga Akiyo, Honda Masato, Qiu Xuchun, Shimasaki Yohei, Kang Ik Joon, Oshima Yuji, Combined effect of anthracene and polyethylene microplastics on swimming speed and cytochrome P4501A monooxygenase expression of Java medaka (Oryzias javanicus), ECOTOXICOLOGY, 10.1007/s10646-023-02700-4, 32, 3, 948-957, 2023.09.
2. Takai Yuki, Izumi Mutsumi, Motoyama Yuriko, Shimasaki Yohei, Oshima Yuji, Kang Ik Joon, Peek-A-Boo Test: A Simple Test for Assessing the Effect of Anxiolytics on Fish Behavior, ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 10.1002/etc.5713, 42, 11, 2358-2363, 2024.05.
3. Honda Masato,Qiu Xuchun,Lydia Undap Suzanne,Kimura Takeshi,Hori Tsuguhide,Shimasaki Yohei,Oshima Yuji, Contamination of Heavy Metals, Polychlorinated Dibenzo-p-Dioxins/Furans and Dioxin-Like Polychlorinated Biphenyls in Wharf Roach Ligia spp. In Japanese Intertidal and Supratidal Zones, APPLIED SCIENCES-BASEL, 10.3390/app11041856, 11, 4, 2021.02.
4. Yoshida M,Shimasaki Y,Inokuchi D,Nakazato A,Otake S,Qiu XC,Mukai K,Foloni-Neto H,Kato H,Honda S,Oshima Y, A New Fluorometer to Detect Harmful Algal Bloom Species and its Application as a Long-Term HABs Monitoring Tool, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 2021.02.
5. Takahashi Yukari,Kawago Umi,Shimasaki Yohei,Oshima Yuji,Suzuki Nobuo, Simple Analysis Method of Hexavalent Chromium in Soil Using a Portable Device, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 66, 1, 53-56, 2021.02.
6. Kato-Unoki Yoko,Takai Yuki,Nagano Yosuke,Matsunaga Satoshi,Enoki Shintaro,Takamura Takumi,Kim Sangwan,Kinoshita Masato,Kitano Takeshi,Shimasaki Yohei,Oshima Yuji, Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka, Oryzias latipes, MARINE POLLUTION BULLETIN, 10.1016/j.marpolbul.2020.111601, 160, 2020.11.
7. Xuchun Qiu,Souvannasing Saovany,Yuki Takai,Aimi Akasaka,Yoshiyuki Inoue,Naoaki Yakata,Yangqing Liu,Mami Waseda,Yohei Shimasaki,Yuji Oshima, Quantifying the vector effects of polyethylene microplastics on the accumulation of anthracene to Japanese medaka (Oryzias latipes), Aquatic Toxicology, 10.1016/j.aquatox.2020.105643, 228, 105643-105643, 2020.11.
8. Min Wu,Xuchun Qiu,Chen Chen,Kun Chen,Ming Li,Hai Xu,Xiangyang Wu,Yohei Shimasaki,Yuji Oshima, Short-term and persistent impacts of sublethal exposure to diazepam on behavioral traits and brain GABA levels in juvenile zebrafish (Danio rerio), Science of the Total Environment, 10.1016/j.scitotenv.2020.140392, 740, 140392-140392, 2020.10.
9. Qiu, Xuchun,Takamura Takumi,Enoki Shintaro,Kato-Unoki Yoko,Takai Yuki,Nagano Yosuke,Kinoshita Masato,Kitano Takeshi,Shimasaki Yohei,Oshima Yuji, Detoxification roles of tributyltin-binding protein type 2 in Japanese medaka (Oryzias latipes) exposed to tributyltin, MARINE POLLUTION BULLETIN, 10.1016/j.marpolbul.2020.111445, 159, 2020.10.
10. Mukai Koki,Shimasaki Yohei,Qiu Xuchun,Kato-Unoki Yoko,Chen Kun,Takai Yuki,Khanam Ruhina Margia,Chairil Abrianna Elke,Oshima Yuji, Gene expression stability of candidate reference genes under different culture conditions for quantitative PCR in the raphidophyteChattonella marina, PHYCOLOGIA, 10.1080/00318884.2020.1816041, 59, 6, 556-565, 2020.11.
11. Assas Mona,Qiu Xuchun,Chen Kun,Ogawa Hijiri,Xu Hai,Shimasaki Yohei,Oshima Yuji, Bioaccumulation and reproductive effects of fluorescent microplastics in medaka fish, MARINE POLLUTION BULLETIN, 10.1016/j.marpolbul.2020.111446, 158, 2020.09.
12. Takai Yuki,Mizoguchi Naohiro,Kinoshita Masato,Qiu Xuchun,Shimasaki Yohei,Oshima Yuji, Establishment of a Japanese medaka (Oryzias latipes) transgenic line expressing Takifugu rubripes pufferfish saxitoxin and tetrodotoxin binding protein 1, and evaluation of tributyltin toxicity via in ovo nanoinjection, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 10.1016/j.cbpc.2020.108785, 234, 2020.08.
13. Qiu Xuchun,Mukai Koki,Shimasaki Yohei,Wu Min,Chen Chen,Lu Yijiang,Ichinose Hirofumi,Nakashima Takashi,Kato-Unoki Yoko,Oshima Yuji, Diurnal variations in expression of photosynthesis-related proteins in the harmful Raphidophyceae Chattonella marina var. antiqua, JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, 10.1016/j.jembe.2020.151361, 527, 2020.06.
14. Xuchun Qiu, Yukihiko Matsuyama, Mitsuhiro Furuse, Yohei Shimasaki, Yuji Oshima, Effects of Chattonella antiqua on the swimming behavior and brain monoamine metabolism of juvenile yellowtail (Seriola quinqueradiata), Marine Pollution Bulletin, 10.1016/j.marpolbul.2020.110896, 152, 2020.03.
15. Xu H,Ding YL,Qiu XC,Shimasaki Y,Oshima Y, Quantitative Proteomic Analysis of Nile Tilapia Brain Following Sub-Chronically Exposed to Toxic Cyanobacteria Microcystis aeruginosa, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 65, 2, 283-288, 2020.02.
16. Takai Y,Tanoue W,Qiu XC,Takaku H,Kang IJ,Shimasaki Y,Honjo T,Oshima Y, Effects of Tributyltin and Diazinon on the Intertidal Marine Harpacticoid Copepod Tigriopus japonicus, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 65, 2, 289-294, 2020.02.
17. Chen K,Iwasaki N,Qiu XC,Xu H,Takai Y,Tashiro K,Shimasaki Y,Oshima Y, Adipogenesis of Perfluorooctanesulfonate (PFOS) on Japanese Medaka (Oryzias Latipes) Embryo Using Ovo-nanoinjection-mRNA Seq Analysis, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 65, 2, 295-303, 2020.02.
18. MRM. Khanam,Yohei Shimasaki,Michito Tsuyama,Hiroshi Goto,Xuchun Qiu,Koki Mukai,Yuji Oshima, Presumption of toxic mechanism of tributyltin on photosystem in marine diatoms by comparison to diuron as a reference agent through chlorophyll a fluorescence transient analysis, Japanese Journal of Environmental Toxicology, doi.org/10.11403/jset.22.13, 22, 1, 13-29, 2019.12.
19. Mukai K,Shimasaki Y,Qiu XC,Kato-Unoki Y,Chen K,Khanam MRM,Oshima Y, Effects of light and hydrogen peroxide on gene expression of newly identified antioxidant enzymes in the harmful algal bloom species Chattonella marina, European Journal of Phycology, 10.1080/09670262.2019.1576062, 54, 3, 393-403, 2019.07.
20. Qiu XC, Kim S, Kang IJ, Hano T, Shimasaki Y, Oshima Y, Combined toxicities of tributyltin and polychlorinated biphenyls on the development and hatching of Japanese medaka (Oryzias latipes) embryos via in ovo nanoinjection, CHEMOSPHERE, 225, 927-934, 2019.06.
21. Qiu XC, Iwasaki N, Chen K, Shimasaki Y, Oshima Y, Tributyltin and perfluorooctane sulfonate play a synergistic role in promoting excess fat accumulation in Japanese medaka (Oryzias latipes) via in ovo exposure, CHEMOSPHERE, 220, 687-695, 2019.04.
22. Takai Y, Chen K, Yoshitake S, Ogino Y, Tashiro K, Shimasaki Y, Oshima Y, Maternal Transcriptome Analysis of Medaka (Oryzias latipes) Embryos by Use of mRNA-seq, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 2019.02.
23. Amornsakun T, Srithongthum S, Promkaew P, bin Hassan A, Matsubara H, Takeuchi Y, Mukai K, Shimasaki Y, Oshima Y, Suzuki N, Effects of Water Salinity on the Egg Hatching, Growth, and Survival of Larvae and Fingerlings of Climbing Perch, Anabas testudineus, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 2019.02.
24. Qiu XC, Wu M, Mukai K, Shimasaki Y, Oshima Y, Effects of Elevated Irradiance, Temperature, and Rapid Shifts of Salinity on the Chlorophyll a Fluorescence (OJIP) Transient of Chattonella marina var. antiqua, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 2019.02.
25. Kato-Unoki Y、Takai Y、Kinoshita M、Mochizuki T、Tatsuno R、Shimasaki Y、Oshima Y, Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes , TOXICON , 153, 58-61, 2018.10.
26. Tasmin R、Shimasaki Y、Tsuyama M、Qiu XC、Khalil F、Mukai K、Khanam MRM、Yamada N、Fukuda S、Kang IJ、Oshima Y, Effects of water temperature and light intensity on the acute toxicity of herbicide thiobencarb to a green alga, Raphidocelis subcapitata, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 25, 25, 25363-25370, 2018.09.
27. Xuchun Qiu, Sayaka Nomichi, Kun Chen, Masato Honda, Ikjoon Kang, Yohei Shimasaki, Yuji Oshima, Short-term and persistent impacts on behaviors related to locomotion, anxiety, and startle responses of Japanese medaka (Oryzias latipes) induced by acute, sublethal exposure to chlorpyrifos, Aquatic Toxicology, 10.1016/j.aquatox.2017.09.012, 192, 148-154, 2017.01, Although most exposures to chlorpyrifos (CPF) in natural flowing waters are brief and episodic, there have been a few reports of the persistence of abnormal fish behaviors caused by such acute exposure. The present study focused on the behavioral and biochemical responses of Japanese medaka (Oryzias latipes) to acute, sublethal exposure to CPF, as well as the persistence of the effects during a 3-week recovery test in CPF-free water. The medaka became hyperactive and exhibited an elevated anxiety state after a 4-day exposure to 0.024 mg/L of CPF, but they recovered from these abnormal behavioral responses within 7 days of recovery treatment. In contrast, persistent impacts on some startle responses to a sudden stimulation (induced by a ball drop) were observed in medaka exposed to CPF. The reaction latency did not change immediately after the 4-day exposure, but was significantly prolonged by as much as 21 days after the termination of exposure. The post-stimulus swimming distance within 5 s significantly decreased on the day immediately after the 4-day exposure, but it significantly increased after 7 days of recovery treatment. The activity of acetylcholinesterase (AChE) in the brains of medaka was significantly inhibited on the day immediately after the 4-day exposure, but it returned to 80% and 110% of that in control fish on days 7 and 21 of the recovery period, respectively. However, AChE activities in the eyes of exposed medaka were persistently inhibited and declined to 33%, 71%, and 72% of that in control fish on days 0 (immediately after the 4-day exposure), 7, and 21 of recovery, respectively. Correlation analysis suggested that the changes of AChE activities in the brains of medaka may underlie some of the observed acute behavioral changes, and the changes of AChE activities in the eyes may contribute to the persistence of the abnormalities in the reaction latency of the startle response. Our findings suggest that medaka need a long time to recover from acute, sublethal exposure to CPF, and the persistence of the behavioral abnormalities might affect their fitness in natural habitats..
28. Xuchun Qiu, Suzanne Lydia Undap, Masato Honda, Toshio Sekiguchi, Nobuo Suzuki, Yohei Shimasaki, Hironori Ando, Waka Sato-Okoshi, Toshihiro Wada, Tomoki Sunobe, Satoshi Takeda, Hiroyuki Munehara, Hisashi Yokoyama, Noriyuki Momoshima, Yuji Oshima, Pollution of radiocesium and radiosilver in wharf roach (Ligia sp.) by the Fukushima Dai-ichi Nuclear Power Plant accident, Journal of Radioanalytical Chemistry, 10.1007/s10967-016-4879-1, 311, 1, 121-126, 2017.01, Radionuclide concentrations in wharf roaches inhabiting coastal areas of Honshu, Japan, were investigated in October 2011 and June 2012. Relative high concentrations of 110mAg (2.1–127�Bq�kg-wet−1), 134Cs (2.6–61�Bq�kg-wet−1), and 137Cs (3.5–92�Bq�kg-wet−1) were detected in specimens from the eastern Honshu areas. Significantly lower 137Cs concentrations (0.7–1.6�Bq�kg-wet−1) were detected in specimens from western and northern Honshu. The decay-corrected 137Cs concentration was significantly inversely correlated with the distance from the Fukushima Dai-ichi Nuclear Power Plant. Thus, wharf roach may serve as a good bioindicator for monitoring radioactive contamination of its habitats..
29. Mst Ruhina Margia Khanam, Yohei Shimasaki, Md Zahangir Hosain, Koki Mukai, Michito Tsuyama, Xuchun Qiu, Rumana Tasmin, Hiroshi Goto, Yuji Oshima, Diuron causes sinking retardation and physiochemical alteration in marine diatoms Thalassiosira pseudonana and Skeletonema marinoi-dohrnii complex, Chemosphere, 10.1016/j.chemosphere.2017.02.054, 175, 200-209, 2017.01, The present research investigated the effect of diuron on sinking rate and the physiochemical changes in two marine diatoms, Thalassiosira pseudonana (single-celled species) and Skeletonema marinoi-dohrnii complex (chain-forming species). The results revealed that the sinking rate of both diatoms exposed to diuron at a level of 50% effective concentration for growth (EC50) decreased significantly compared with the control. Photosynthetic performance (Fv/Fm and PIABS) of both diatoms also decreased significantly with diuron exposure. The number of cells per chain in S. marinoi-dohrnii decreased significantly with diuron treatment, but T. pseudonana cell diameter remained stable. Neutral lipid concentration per cell was significantly higher compared with control at 72 h in both diatom species exposed to EC50 level diuron. And water-soluble protein concentration per cell at 72 h was lower than control in the T. pseudonana EC50 group only. These biochemical changes may decrease specific gravity of cells and seems to cause a decreased sinking rate in diatoms. The positive significant correlation between the numbers of cells per chain and sinking rate in S. marinoi-dohrnii indicated that chain length is also an important factor in sinking rate regulation for chain-forming diatoms. Thus, our present study suggested that suppression of photosynthetic performance and the resultant physiochemical changes induced the decreased sinking rate that may inhibit the normal survival strategy (avoidance from the surface layer where strong light either causes photo-inhibition or interrupts resting cell formation). Therefore, the use of antifouling agents should be considered for the sustainable marine environment..
30. Dao M. Hai, Xuchun Qiu, Hai Xu, Masato Honda, Mitsuyasu Yabe, Kiwao Kadokami, Yohei Shimasaki, Yuji Oshima, Contaminants in Liquid Organic Fertilizers Used for Agriculture in Japan, Bulletin of Environmental Contamination and Toxicology, 10.1007/s00128-017-2081-y, 99, 1, 131-137, 2017.01, To provide an overview of anthropogenic contaminants in liquid organic fertilizers (LOFs), products from four biogas plants in Kyushu, Japan, were analyzed for a wide range of contaminants, including copper, cadmium, tributyltin (TBT), dibutyltin (DBT), perfluorooctane sulfonate, 952 semi-volatile organic compounds, and 89 antibiotics. The highest concentrations of copper (31.1 mg/L) and cadmium (0.08 mg/L) were found in LOFs from the Hita biogas plant. Only ofloxacin and sulfapyridine were detected in total 89 antibiotics screened. TBT, DBT, and perfluorooctane sulfonate were present at low concentrations in the LOFs from all four locations. Among the 952 semi-volatile organic compounds, 78 compounds were detected in at least one sample and were present at concentrations between 1.2 and 139.6 mg/L. On the basis of comparisons with previous studies and quality standards for the use of organic fertilizers, the concentrations of contaminants in the studied LOFs indicate that they might be safe for agricultural purposes..
31. Kun Chen, Yuki Tsutsumi, Shuhei Yoshitake, Xuchun Qiu, Hai Xu, Yasuyuki Hashiguchi, Masato Honda, Kosuke Tashiro, Kei Nakayama, Takeshi Hano, Nobuo Suzuki, Kazuichi Hayakawa, Yohei Shimasaki, Yuji Oshima, Alteration of development and gene expression induced by in ovo-nanoinjection of 3-hydroxybenzo[c]phenanthrene into Japanese medaka (Oryzias latipes) embryos, Aquatic Toxicology, 10.1016/j.aquatox.2016.11.020, 182, 194-204, 2017.01, Benzo[c]phenanthrene (BcP) is a highly toxic polycyclic aromatic hydrocarbon (PAHs) found throughout the environment. In fish, it is metabolized to 3-hydroxybenzo[c]phenanthrene (3-OHBcP). In the present study, we observed the effects of 1 nM 3-OHBcP on the development and gene expression of Japanese medaka (Oryzias latipes) embryos. Embryos were nanoinjected with the chemical after fertilization. Survival, developmental stage, and heart rate of the embryos were observed, and gene expression differences were quantified by messenger RNA sequencing (mRNA-Seq). The exposure to 1 nM 3-OHBcP accelerated the development of medaka embryos on the 1st, 4th, and 6th days post fertilization (dpf), and increased heart rates significantly on the 5th dpf. Physical development differences of exposed medaka embryos were consistent with the gene expression profiles of the mRNA-Seq results for the 3rd dpf, which show that the expression of 780 genes differed significantly between the solvent control and 1 nM 3-OHBcP exposure groups. The obvious expression changes in the exposure group were found for genes involved in organ formation (eye, muscle, heart), energy supply (ATPase and ATP synthase), and stress-response (heat shock protein genes). The acceleration of development and increased heart rate, which were consistent with the changes in mRNA expression, suggested that 3-OHBcP affects the development of medaka embryos. The observation on the developmental stages and heart beat, in ovo-nanoinjection and mRNA-Seq may be efficient tools to evaluate the effects of chemicals on embryos..
32. Toshio Sekiguchi, Shigehiro Kuraku, Kaori Tatsumi, Yohei Shimasaki, Yuji Oshima, Nobuo Suzuki, Identification and molecular characterization of the stanniocalcin family gene from the inshore hagfish, eptatretus burger, Journal of the Faculty of Agriculture, Kyushu University, 62, 1, 93-98, 2017.02, Stanniocalcin (STC) is a glycoprotein hormone that was first isolated from teleost corpuscles of Stannius. STC is involved in the decrease in plasma Ca'Jt level in teleosts. Comparative studies between invertebrate chordate STC and two paralogous vertebrate STCs suggest that the chordate ancestral gene of STC diverged into two paralogous genes in the evolution of vertebrates. However, this evolutionary scenario remains unclear. To clarify the diversification process of vertebrate STC, we made an attempt to isolate orthologs of STC in extant jawless fishes (cyclostomes) located most distantly in the vertebrate tree of life. In the present study, we searched the STC-like sequence by performing degenerate PCR in the inshore hagfish Eptatretus burgeri. We identified a STC homolog, designated as Eb-STC. Our molecular phyloge- netic analysis confirmed that Eb-STC is a member of the STC family. The comparison of amino acid sequences revealed that Eb-STC is similar to gnathostome STC1 and 2, in that Eb-STC shares the 10 conserved cysteine residues that form intramolecular disulfide bonds. Eb-STC and gnathostome STCs possess the conserved -glycosylated site. However, typical features discriminated between STC1 and 2, such as the distinct position of the cysteine residue associated with the intermolecular dimer, were not observed in Eb-STC. Tissue distribution analysis revealed that Eb-STC is expressed in various organs, but not in the brain. This expression pattern resembles that of teleost STC1 and 2, suggesting that Eb-STC acts as a paracrine factor. To the best of our knowledge, this is the first report on the identification and molecular characterization of STC in cyclostomes..
33. Xuchun Qiu, Kokl Mukai, Yohei Shimasaki, Michito Tsuyama, Tadashi Matsubara, Takashi Nakashima, Hirofumi Ichinose, Yu Nakajima, Ibuneo Honjo, Yuji Oshima, Variations in the expression of photosynthesis-related proteins in field Chattonella marina cells during a harmful algal bloom, Journal of the Faculty of Agriculture, Kyushu University, 62, 2, 373-380, 2017.09, Time series variations in protein expression profiles in field Chattonella marina cells were investigated during a HAB occurred in the inner part of Ariake Sea, Japan (5-14 September, 2012). This study aimed to gather information on the molecular mechanisms underlying the physiological responses in field C. marina population during HAB. Proteomic analysis showed that the abundance of -37% protein spots (40 out of 108 detected from 2-DE gel images) significantly varied with the sampling date. Significant decreases in the abundances of proteins involved in photosystem II (LHCP 4), electron transfer chain (Cyt c553), Calvin cycle (GAPDH), and chloroplast antioxidant system (2-Cys Prx) were observed as the bloom progressed, suggesting the efficiencies of those photosynthetic pathways declined during the bloom. In addition, the abundances of the above proteins showed significant positive correlations with the Fv/Fm ratio and growth rate of C. marina and with DIN concentrations (except LHCP 4). Our findings suggested that declined expressions of those photosynthesis-related proteins presented some molecular foundation of the decreases in Fv/Fm ratio and growth rate of C. marina during the bloom, and also provided insight into mechanistic links between the external/internal factors and physiological responses of C. marina that may ultimately dictate the ecology of the bloom..
34. Yoichi Ease, Shouzo Ogiso, Takahiro Ikari, Tbshio Sekigucm, Yuichi Sasayama, Yoichlro Kitani, Yohei Shimasaki, Yuji Osiiima, Akira Kambegawa, Yoshiaki Tabuchi, Atsuhiko Hattori, Nobuo Suzuki, Immunoreactive calcitonin cells in the nervous system of polychaete Perinereis aibuhitensis, Journal of the Faculty of Agriculture, Kyushu University, 62, 2, 381-385, 2017.09, We examined immunoreactive calcitonin (iCT) cells in the nervous system of the polychaete Perinereis aibuhitensis using immunohistochemical methods. We found the most iCT cells (53-70 cells) in the cerebral ganglion. We also detected iCT cells (4-6 cells) in the subpharyngeal ganglion. Furthermore, it was noted that iCT cells were present bilaterally in each segment of the ventral nerve cord. These results suggest that iCT cells play some functional roles in the nervous system. Next, the molecular weight (MW) of the iCT substance was examined using the western blotting method. Cerebral ganglia were collected from 200 individuals. These ganglia were homogenized and centrifuged. The separated supernatants were treated with 66% acetone and then dialyzed to remove low MW substances (less than 2,000) at 4°C. After lyophilization, the sample was reconstituted with 1 M acetic acid and then fractionated with an ultrafiltration membrane system into MWs of 3,000 to 10,000. Thereafter, the specimen was separated by SDS-polyacrylamide gel electrophoresis and analyzed by western blotting with anti-salmon calcitonin (CT) antiserum. Our results indicated that the MW of the iCT substance was close to that of teleost fish CT (3.5 kDa). The annelid Capitella teleta has two genes encoding CT-like peptides. This suggests that the iCT substance in P. aibuhitensis includes amino acid residues similar to fish CT and belongs to the CT family..
35. Masato Honda, Hiroaki Gunjikake, Shigeaki Matsut, Xuchun Qiu, Yohei Shimasaki, Yuji Oshima, Effect of repeated Exposure to low oxygen on respiratory metabolism and vertical movements in the pen shell Atrina lischkeana, Journal of the Faculty of Agriculture, Kyushu University, 62, 2, 387-392, 2017.09, We exposed pen shells (Atrina lischkeana) to repeated periods of low-oxygen for 30 d and measured their survival, behavior, glycogen content, and organic acid concentration. Exposure to low-oxygen had no effect on survival. Animals moved upward above the sediment under low-oxygen conditions and downward under aerobic conditions, repeating these movements for 19 d. The difference in shell height above the sediment before and after low-oxygen exposure gradually decreased; after 20 d there was no significant difference. Low-oxygen exposure also resulted in a significant decrease in glycogen content and in concentrations of malate and fumarate. Thus, repeated exposure of pen shells to low-oxygen conditions reduces glycogen content, alters metabolism, and affects vertical movements, all of which may cause ecological dysfunction. We suggest that hypoxia might be one factor contributing to the collapse of pen shell resources..
36. Fatma Khalil, Xuchun Qiu, Ikjoon Kang, Ismail Abo-Ghanema, Yohei Shimasaki, Yuji Oshima, Comparison of social behavior responses of Japanese medaka (Oryzias latipes) to lethal and sublethal chlorpyrifos concentrations at different exposure times, Ecotoxicology and Environmental Safety, 10.1016/j.ecoenv.2017.07.007, 145, 78-82, 2017.11, Chlorpyrifos (CPF) is one of the most widely used insecticides and has been found in both urban and rural water bodies. In this study, we studied variations in the social behavioral patterns, swimming behavior, and brain acetylcholinesterase (AChE) activity of Japanese medaka exposed to lethal (0.12 mg/L) and sublethal (0.012 mg/L) concentrations of CPF after different exposure times. Group behavior performance (schooling, shoaling, and solitary) was determined on day 4 of lethal exposure and on days 4, 8, and 12 of sublethal exposure. Swimming speed and brain AChE activity were measured on days 4, 8, 12, 16, and 20 of sublethal CPF exposure. We observed significant decreases in social behavior and swimming speed (i.e., hypoactivity) in fish exposed to lethal CPF concentrations for 4 days. At the sublethal concentration, there was increased schooling duration and hyperactivity of fish on day 8 but not on day 4. In contrast, 12 days of sublethal CPF exposure resulted in social behavior responses similar to those after 4 days’ lethal exposure, i.e., significant decreases in schooling frequency and duration with a notable increase in duration of solitary behavior. Brain AChE activity was inhibited in a time-dependent manner. Altered fish behavior in response to organophosphorus pesticides such as CPF may be mediated by more than AChE inhibition alone..
37. Xuchun Qiu, Wataru Tanoue, Atsushi Kawaguchi, Takashi Yanagawa, Masanori Seki, Yohei Shimasaki, Tsuneo Honjo, Yuji Oshima, Interaction patterns and toxicities of binary and ternary pesticide mixtures to Daphnia magna estimated by an accelerated failure time model, Science of the Total Environment, 10.1016/j.scitotenv.2017.07.034, 607-608, 367-374, 2017.12, Organisms in natural environments are often exposed to a broad variety of chemicals, and the multi-chemical mixtures exposure may produce significant toxic effects, even though the individual chemicals are present at concentrations below their no-observed-effect concentrations. This study represents the first attempt that uses the accelerated failure time (AFT) model to quantify the interaction and toxicity of multi-chemical mixtures in environmental toxicology. We firstly conducted the acute immobilization tests with Daphnia magna exposed to mixtures of diazinon (DZN), fenitrothion (MEP); and thiobencarb (TB) in single, binary, and ternary formulations, and then fitted the results to the AFT model. The 48-h EC50 (concentration required to immobilize 50% of the daphnids at 48 h) values for each pesticide obtained from the AFT model are within a factor of 2 of the corresponding values calculated from the single pesticide exposure tests, indicating the methodology is able to provide credible toxicity values. The AFT model revealed either significant synergistic (DZN and MEP; DZN and TB) or antagonistic (MEP and TB) interactions in binary mixtures, while the interaction pattern of ternary mixture depended on both the concentration levels and concentration ratios of pesticides. With a factor of 2, the AFT model accurately estimated the toxicities for 78% of binary mixture formulations that exhibited significant synergistic effects, and the toxicities for all the ternary formulations. Our results showed that the AFT model can provide a simple and efficient way to quantify the interactions between pesticides and to assess the toxicity of their mixtures. This ability may greatly facilitate the ecotoxicological risk assessment of exposure to multi-chemical mixtures..
38. Masato Honda, Akemi Muta, Akinari Shimazaki, Taiki Akasaka, Michiyasu Yoshikuni, Yohei Shimasaki, Yuji Oshima, High concentrations of perfluorooctane sulfonate in mucus of tiger puffer fish Takifugu rubripes
a laboratory exposure study, Environmental Science and Pollution Research, 10.1007/s11356-017-0537-6, 25, 2, 1551-1558, 2018.01, Distribution of perfluorooctane sulfonate (PFOS) was investigated in tissues (plasma, blood clot, mucus, skin, liver, muscle, and gonad) of tiger puffer fish Takifugu rubripes. A single dose of PFOS was intraperitoneally injected at 0.1 mg/kg body weight with samples taken over a 14-day period. The highest concentration of PFOS was found in the plasma, 861 ng/mL at 14 days, followed by the mucus, liver, blood clot, gonads, muscles, and skin of fish. A gradual upward trend in PFOS concentration was observed in the mucus and liver whereas there was no change in the plasma, blood clot, gonad, muscle, and skin after the initial increase in PFOS concentrations following injection. No significant trend for estimated total PFOS content in whole body was observed during the experimental period. Relatively high concentrations of PFOS (690 ng/g ww after 14 days) were detected in body surface mucus that continuously oozes from the skin. These results may suggest that mucus is one of the elimination pathways of PFOS in tiger puffer fish..
39. Koki Mukai, Ayano Teramoto, Xuchun Qiu, Yohei Shimasaki, Yoko Kato-Unoki, Man Lee, Naohiro Mizoguchi, Mst Ruhina Margia Khanam, Hina Satone, Tsuneyuki Tatsuke, Takahiro Kusakabe, Yuji Oshima, Gene structure and cDNA sequence of 2-Cys peroxiredoxin in the harmful algal bloom species Chattonella marina and its gene transcription under different light intensities, European Journal of Phycology, 10.1080/09670262.2017.1346206, 53, 1, 29-38, 2018.01, We investigated the gene structure and predicted amino acid sequence of the antioxidant enzyme 2-Cys peroxiredoxin (2-Cys Prx) in the raphidophyte Chattonella marina, which is a harmful algal bloom (HAB) species. The open reading frame of 2-Cys Prx was 585 bp long and encoded a protein consisting of 195 amino acids. The putative amino acid sequence contained two cysteine residues located at the 49th and 170th amino acid positions from the N-terminal methionine residue. The sequence also possessed 2-Cys Prx characteristic motifs, F (FFYPLDFTFVCPTEI) and EVCP. The position of the 2-Cys Prx gene relative to several others (ycf59–2-CysPrx–rpl35–rpl20) was the same as that found in the chloroplast genome in the raphidophyte Heterosigma akashiwo. Upstream of the 2-Cys Prx gene, possible TATA and GGA motifs recognized by nuclear-encoded plastid RNA polymerase (NEP), and a possible -10 box and -35 box recognized by plastid-encoded plastid RNA polymerase (PEP) were observed. We measured the transcript levels of 2-Cys Prx in C. marina cells grown under three different light intensities (0, 100, 1000 µmol photons m–2 s–1, 14-h light/8-h dark photoperiod) by quantitative PCR. The 2-Cys Prx transcript level in cells grown under the highest light intensity on day 3 was threefold that on day 0 but two lower light intensities resulted in relatively stable transcription levels. The 2-Cys Prx transcript level was significantly positively related to the H2O2 concentration per cell and the H2O2 scavenging activity per cell. These results suggest that C. marina 2-Cys Prx functions in the chloroplast and its transcription could be regulated by both NEP and PEP. Moreover, the 2-Cys Prx transcript level might increase to remove excessive H2O2 produced under strong light conditions in order to maintain cell proliferation activity..
40. Masato Honda, Xuchun Qiu, Jiro Koyama, Seiichi Uno, Suzanne Lydia Undap, Yohei Shimasaki, Yuji Oshima, The Wharf Roach, Ligia sp., A Novel Indicator of Polycyclic Aromatic Hydrocarbon Contamination in Coastal Areas, International Journal of Environmental Research, 10.1007/s41742-017-0063-x, 12, 1, 2018.03, We investigated polycyclic aromatic hydrocarbon (PAH) pollution of the intertidal and supratidal zone using the wharf roach (Ligia sp.) as a novel environmental indicator. PAH and alkylated PAH (alkPAH) concentrations in wharf roaches collected from nine sampling sites on the central and northern coast of Japan in November 2011 were analyzed. Total alkPAH concentrations were higher than those of PAHs at all sampling sites. Seven sampling sites had relatively low total concentrations of PAHs and alkPAHs (57.7–130 and 276–770 ng/g-dry, respectively); they included sites in Miyagi Prefecture (St. 5 and 9) that were seriously damaged by the Great East Japan Earthquake and by the following Tsunami on 11 March 2011. Two sampling sites (St. 2: Kominato, Aomori Prefecture; St. 3: Usushiri, Hokkaido) had higher concentrations of total PAHs and alkPAHs (PAHs, 1025 and 591 ng/g-dry, respectively; alkPAHs, 1490 and 1160 ng/g-dry, respectively). Diagnostic ratio calculation showed that St. 2 and 3 had PAHs of pyrolytic origin, possibly from vehicle exhaust gas or industrial fuel combustion, or both. The concentrations of these pollutants in the wharf roach were similar to those in bivalves and therefore likely reflected PAH pollution at the collection sites. The wharf roach may be a suitable candidate indicator of PAH pollution, not only in the intertidal zone inhabited by the currently used bivalve indicators, but also in the supratidal zone..
41. Hina Satone, Shohei Naotaka, JAE MAN LEE, Yohei Shimasaki, takahiro kusakabe, Shun-ichiro Kawabata, Yuji Oshima, Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes, Toxicon, 125, 50-52, 2017.01.
42. Hongbo Liu, Xiubao Chen, Yanping Su,, Ik Joon KANG, Yohei Shimasaki, Yuji Oshima, Jian Yang, Calcium and Magnesium Ions on Acute Copper Toxicity to Glochidia and Early Juveniles of the Chinese Pond Mussel Anodonta woodiana., Bulletin of Environmental Contamination and Toxicology, 97, 4, 504-509, 2016.10.
43. Hashiguchi Y, JAE MAN LEE, Shiraishi M, Komatsu S, Miki S, Yohei Shimasaki, Noritaka Mochioka, takahiro kusakabe, Yuji Oshima, Characterization and evolutionary analysis of tributyltin-binding protein and pufferfish saxitoxin and tetrodotoxin-binding protein genes in toxic and nontoxic pufferfishes, JOURNAL OF EVOLUTIONARY BIOLOGY, 28, 5, 1103-1118, 2015.05.
44. Yohei Shimasaki, Michito Tsuyama, Tasmin R, Khanam MRM, Qiu X, Yuji Oshima, Comparative study of toxicological responses of chlorophyll-related parameters in the marine diatom Skeletonema sp. exposed to tributyltin and Irgarol., Japanese Journal of Environmental Toxicology, 18, 1, 1-10, 2015.06.
45. Honda M, Muta A, Akasaka T, Inoue Y, Yohei Shimasaki, Kannan K, NOZOMU OKINO, Yuji Oshima, Identification of perfluorooctane sulfonate binding protein in the plasma of tiger pufferfish Takifugu rubripes , ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY , 104, 409-413, 2014.06.
46. Tasmin R, Yohei Shimasaki, Qiu X, Honda M, Michito Tsuyama, Naotaka YAMADA, Shinji FUKUDA, Yuji Oshima, Elevated temperatures and low nutrients decrease the toxicity of diuron for growth of the green alga Pseudokirchneriella subcapitata., Japanese Journal of Environmental Toxicology, 17, 5-10, 2014.08.
47. Yohei Shimasaki et al, Thiobencarb Herbicide Reduces Growth, Photosynthetic Activity, and Amount of Rieske Iron-Sulfur Protein in the Diatom Thalassiosira pseudonana , JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY , 21, 2, 1064-1070, 2013.09.
48. Tasmin, Rumana et al, Elevated water temperature reduces the acute toxicity of the widely used herbicide diuron to a green alga, Pseudokirchneriella subcapitata , ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH , 21, 2, 1064-1070, 2014.01.
49. Khalil F, Kang IJ, Undap S, Tasmin R, Yohei Shimasaki, Yuji Oshima, Alterations in social behavior of Japanese medaka (Oryzias latipes) in response to sublethal chlorpyrifos exposure, Chemoshphere, 92, 125-130, 2013.04.
50. Undap S, Nirmala K, Miki S, Inoue S, Honda M, Yohei Shimasaki, Yuji Oshima, High tributyltin contamination in sediments from ports in Indonesia and northern Kyushu, Japan , Journal of the Faculty of Agriculture, Kyushu University , 58, 131-135, 2013.02.
51. Qiu, Xuchun, Yohei Shimasaki, Michito Tsuyama, Yamada T, Kuwahara R, Kawaguchi M, Honda M, Gunjikake H, Tasmin R, Shimizu M, Sato Y, Kato-Unoki Y, Takashi Nakashima, Matsubara T, Yamasaki Y, Hirofumi Ichinose, Wariishi H, Honjo T, Yuji Oshima, Growth-Phase Dependent Variation in Photosynthetic Activity and Cellular Protein Expression Profile in the Harmful Raphidophyte Chattonella antiqua , BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY , 77, 1, 46-52, 2013.01.
52. S Miki , K Ikeda, Y Oba, H Satone, M Honda, Y Shimasaki, N Onikura, O Arakawa, Y Oshima., Tributyltin in blood of marine fish collected from a coastal area of northern Kyushu, Japan., Mar Poll Bull, 62, 2533-2536, 2011.11.
53. ◦Qiu X, Yamasaki Y, Shimasaki Y, Gunjikake H, Honda H, Kawaguchi M, Matsubara T, Nagasoe S, Etoh T, Matsui S, Honjo T, Oshima Y., Allelopathy of the raphidophyte Heterosigma akashiwo against the dinoflagellate Akashiwo sanguinea is mediated via allelochemicals and cell contact , Marine Ecology-Progress Series, 446, 107-118, 2012.01.
54. Yamasaki Y, Ohmichi Y, Hirose M, Shikata T, Shimasaki Y, Oshima Y, Honjo T., LOW MOLECULAR WEIGHT ALLELOCHEMICALS PRODUCED BY THE DIATOM, Skeletonema costatum, Thalassas , 28, 9-17, 2012.01.
55. Hano T, Oshima Y, Kinoshia M, Tanaka M, Mishima N, Wakamatsu Y, Ozato K, Shi-masaki Y, Honjo T., Evaluation of the effects of ethinylestradiol (EE2) on sexual differentiation in the olvas-GFP/STII-YI medaka (transgenic Oryzias latipes) strain as estimated by proliferative activity of germ cells. , Aquat Toxicol , 104, 177-184, 2011.08.
56. Satone H, Lee JM, Oba Y, Kusakabe T, Akahoshi E, Miki S, Suzuki N, Sasayama Y, Nassef M, Shimasaki Y, Kawabata S, Honjo T, Oshima Y, Tributyltin-binding protein type 1, a lipocalin, prevents inhibition of osteoblastic activity by tributyltin in fish scales , Aquat Toxicol , 103, 79-84, 2011.05.
57. Nakayama K, Sei N,. Handoh I, Shimasaki Y, Honjo T, Oshima Y. , Effects of polychlorinated biphenyls on liver function and sexual characteristics in Japanese medaka (Oryzias latipes). , Mar Poll Bull , 63, 366-369 , 2011.04.
58. Nassef M, Kato-Unoki Y, Furuta T, Nakayama K, Satone H, Shimasaki Y, Honjo T, Oshima Y., Molecular cloning, sequencing, and gene expression analysis of tributyltin-binding protein type 1 in Japanese medaka fish, Oryzias latipes, Zoological Science , 28, 281-285, 2011.04.
59. Nassef M, Tawaratsumida T, oba Y, Satoe H, Nakayama K, Shimasaki Y, Honjo T, Oshima Y., Induction of tributyltin-binding protein type 2 in Japanese flounder, Paralichthys olivaceus, by exposure to tributyltin-d27, Marine Pollution Bulletin, 62, 412-414, 2011.02.
60. Yamasaki Y., Ohmichi Y., Shikata T., Hirose M., Shimasaki Y., Oshima Y., Honjo T., Species-specific allelopathic effects off the diatom Skeletonema costatum, Thalassas, 27, 21-32, 2011.01.
61. Qiu X, Yamasaki Y, Shimasaki Y, Gunjikake H, Shikata T, Matsubara T, Nagasoe S, Honjo T, Oshima, Growth interactions between raphidophytes Chattonella antiqua and Heterosigma akashiwo, Thalassas, 27, 33-45, 2011.01.
62. Oba Y, Yamauchia A, Hashiguchi Y, Satonea H, Miki S, Nassef M, Shimasaki Y, Kitano T, Nakao M, Kawabata S, Honjo T, Oshima Y, Purification and charac-terization of tributyltin-binding protein of tiger puffer, Takifugu rubripes, Comparative Biochemistry and physilogy C , 153, 17-23, 2011.01.
63. Yamasaki Y., Zou Y., Go J., Shikata T., Matsuyama Y., Nagai K., Shimasaki Y., Yamaguchi K., Oshima Y., Oda T., Honjo T. , Cell-contact-dependent lethal effect of the dinoflagellate Heterocapsa circularisquama on phytoplankton-phytoplankton interactions, Journal of Sea Research, 65, 76-83, 2011.01.
64. Nagasoe S, Shikata T, Yamasaki Y, Matsubara T, Shimasaki Y, Oshima Y, Honjo , Effects of nutrients on growth of the red-tide dinoflagellate Gyrodinium instriatum et Lee and a possible link to blooms of this species Freudenthal, Hydrobiologia , 651, 225-238, 2010.09.
65. Nassef M , Matsumoto S, Seki M, khalil F, Kang IJ, Shimasaki Y, Oshima Y, Honjo, Acute effects of triclosan, diclofenac and carbamazepine on feeding performance of Japanese medaka fish (Oryzias latipes), Chemosphere, 80, 1095-1100, 2010.08.
66. Nassef M, Kim SG, Seki M, Kang IJ, Hano T, Shimasaki Y, Oshima, In ovo nanoinjection of triclosan, diclofenac and carbamazepine affects embryonic development of medaka fish (Oryzias latipes), Chemosphere, 79, 966-973, 2010.05.
67. Yamasaki Y, Nagasoe S, Tameishi M, Shikata T, Zou Y, Jiang Z, Matsubara T, Shimasaki, Yamaguchi K, Oshima Y, Oda T, Honjo T, The role of interactions between Prorocentrum minimum and Heterosigma akashiwo in bloom formation, HYDROBIOLOGIA , 641, 33-44, 2010.09.
68. Hano T, Oshima Y, Kinoshita M, Tanaka M, Wakamatsu Y, Ozato K, Nassef M, Shimasaki Y, Honjo T , In ovo nanoinjection of nonylphenol affects embryonic development of a transgenic see-through medaka (Oryzias latipes), olvas-GFP/STII-YI strain, CHEMOSPHERE, 77: 1594-1599, 2009.12.
69. Nassef M, Matsumoto S, Seki M, Kang IJ, Moroishi J, Shimasaki Y, Oshima Y , Pharmaceuticals and Personal Care Products Toxicity to Japanese Medaka Fish (Oryzias latipes) , JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 54: 407-411, 2009.10.
70. Moroishi J, Kang IJ, Nagafuchi K, Honjo T, Shimasaki Y, Oshima Y , Biological Monitoring to Detect Both Water Pollution and Water Quality Recovery Based on Valve Movements of Freshwater Bivalves (Corbicula japonica) , JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 54: 413-420, 2009.10.
71. Yamasaki Y, Shikata T, Nukata A, Ichiki S, Nagasoe S, Matsubara T, Shimasaki Y, Nakao M, Yamaguchi K, Oshima Y, Oda T, Ito M, Jenkinson IR, Asakawa M, Honjo T , Extracellular polysaccharide-protein complexes of a harmful alga mediate the allelopathic control it exerts within the phytoplankton community, ISME JOURNAL, 3: 808-817, 2009.07.
72. Basti L, Nagai K, Shimasaki Y, Oshima Y, Honjo T, Segawa S, Effects of the toxic dinoflagellate Heterocapsa circularisquama on the valve movement behaviour of the Manila clam Ruditapes philippinarum, AQUACULTURE , 291: 41-47, 2009.06.
73. Shikata T, Nukata A, Yoshikawa S, Matsubara T, Yamasaki Y, Shimasaki Y, Oshima Y, Honjo T., Effects of light quality on initiation and development of meroplanktonic diatom blooms in a eutrophic shallow sea., Marine Biology, 156: 875-889, 2009.04.
74. Tameishi M, Yamasaki Y, Nagasoe S, Shimasakia Y, Oshima Y, Honjo T., Allelopathic effects of the dinophyte Prorocentrum minimumon the growth of the bacillariophyte Skeletonema costatum., Harmful Algae, 8: 421-429., 2009.02.
75. Satone H, Oshima Y, Shimasaki Y, Tawaratsumita T, Oba Y, Takahashi E, Kitano T, Kawabata S, Kakuta Y, Honjo T., Tributyltin-binding protein type 1 has a distinctive lipocalin-like structure and is involved in the excretion of tributyltin in Japanese flounder, Paralichthys olivaceus., Aquatic Toxicology, 90: 292-299. , 2008.12.
76. Shikata T, Yoshikawa S, Matsubara T, Tanoue W, Yamasaki Y, Shimasaki Y, Matsuyama Y, Oshima Y, Jenkinson IR, Honjo T., Growth dyanmics of Heterosigma akashiwo (Raphidophyceae) in Hakata Bay, Japan., European Journal of Phycology, 43: 395-411., 2008.11.
77. Shikata T, Nagasoe S, Matsubara T, Yoshikawa S, Yamasaki Y, Shimasaki Y, Oshima Y, Jenkinson IR, Honjo T., Factors influencing the initiation of blooms of the raphidophyte Heterosigma akashiwo and the diatom Skeletonema costatum in a port in Japan., Limnology and Oceanography, 53: 2503-2518., 2008.11.
78. Shikata T, Nagasoe S, Matsubara T, Yamasaki Y, Shimasaki Y, Oshima Y, Honjo T., Encystment and excystment of Gymnodinium instriatum Freudenthal et al Lee., Journal of Oceanography, 64: 355-365., 2008.06.
79. Kang IJ, Yokota H, Oshima Y, Tsuruda Y, Shimasaki Y, and Honjo T., The effects of methyltestosterone on the sexual development and reproduction of adult medaka (Oryzias latipes)., Aquatic Toxicology, 87: 37-46., 2008.04.
80. Nakayama K, Sei N, Oshima Y, Tashiro K, Shimasaki Y, Honjo T., Alteration of gene expression profiles in the brain of Japanese medaka (Oryzias latipes) exposed to KC-400 or PCB126., Marine Pollution Bulletin, 57: 460-466., 2008.03.
81. Shimasaki Y, Oshima Y, Inoue Y, Shibata H, Nakayama K, Inoue S, Imoto H, Kang IJ and Honjo T, Distribution of tributyltin in tissues of mature Japanese whiting, Sillago japonica and their eggs, J. Fac. Agr. Kyshu Univ, 53:67-72 , 2008.02.
82. Shikata T, Nagasoe S, S. J.Oh, Matsubara T, Yamasaki Y, Shimasaki Y, Oshima Y, Honjo T, Effects of down- and up-shocks from rapid changes of salinity on survival and growith of estuarine phytoplankters. , J. Fac. Agr. Kyushu Univ, 53, 81-87., 2008.02.
83. T. Shikata, S. Nagasoe, T. Matsubara, Y. Yamasaki, Y. Shimasaki, Y. Oshima, T. Uchida, I. R. Jenkinson, T. Honjo. , Encystment and Excystment of Gyrodinium instriatum Freudenthal et Lee., Journal of Oceanography , 64: 355-365, 2008.01.
84. Hano T, Oshima Y, Kim SG, Satonne H, Ooba Y, Kitano T, Inoue S, Shimasaki Y, Honjo T., Tributlyltin causes abnormal development in embryo of medaka, Oryzias latipes., Chemosphere, 69: 927-933., 2007.10.
85. S. J. Oh, D. I. Kim, T. Sajima, Y. Shimasaki, Y. Matsuyama,Y. Oshima, T. Honjo and H. S. Yang, Effects of irradiance of various wavelengths from light-emitting diodes on the growth of the harmful dinoflagellate Heterocapsa circularisquama and the diatom Skeletonema costatum, Fiseries Science, 2007.09.
86. T. Shikata, S. Nagasoe, T. Matsubara, Y. Yamasaki, Y. Shimasaki, Y. Ohshima and T. Honjo , Effects of temperature and light on cyst germination and germinated cell survival of the noxious raphidophyte Heterosigma akashiwo, Harmful Algae , 6 : 700-706, 2007.07.
87. Yasmasaki Y., Nagasoe S., Matsubara T., Shikata T., Shimasaki Y., Oshima Y. and Honjo T., Allelopathic interactions between the bacillariophyte Skeletonema costatum (Greville) Cleve and the raphidophyte Heterosigma akashiwo (Hada) Hada ex Hara et Chihara., Marine Ecology Progress Series , 339: 83-92, 2007.07.
88. Y. Yamasaki, S. Nagasoe, T. Matsubara, T. Shikata, Y. Shimasaki, Y. Oshima and T. Honjo. , Growth inhibition and formation of morphologically abnormal cells of Akashiwo sanguinea (Hirasaka) G. Hansen et Moestrup by cell contact with Cochlodinium polykrikoides Margalef., Marine Biology , 152: 157-163, 2007.07.
89. S. J. Oh, D. S. Park, H. S. Yang, Y. H. Yoon and T. Honjo : , Bioremediation on the benthic layer in polluted inner bay by promotion of microphytobenthos growth using light emitting diode (LED)., J. Korean Soc. Mar. Environ. Engineer. , 10 : 93-101, 2007.06.
90. Y. Oba, Y. Shimasaki, Y. Oshima, H. Satone, T. Kitano, M. Nakao, S. Kawabata and T.Honjo , Purification and characterization of tributyltin binding protein type 2 from plasma of Japanese flounder, Paralichthys olivaceus., J Biochemistry , 2007.05.
91. T. Matsubara, S. Nagasoe, Y. Yamasaki, T. Shikata, Y. Shimasaki, Y. Oshima and T. Honjo., Effects of temperature, salinity, and irradiance on the growth of the dinoflagellate Akashiwo sanguinea., Journal of Experimental Marine Biology and Ecology , 342: 226-230, 2007.04.
92. Inoue, S., Y. Oshima, H. Usuki, M. Hamaguchi, Y. Hanamura, N. Kai, Y. Shimasaki, T. Honjo, Effect of tributyltin on veliger larvae of the Manila clam, Ruditapes philippinarum, Chemosphere, 66 1353- 1357, 2007.01.
93. Nagasoe, S., Toda, S., Shimasaki, Y., Oshima, Y., Uchida, T. and Honjo T., Growth inhibition of Gyrodinium instriatum (Dinophycae) by Skeletonema costatum (Bacillariophyceae)., African Journal of Marine Science, 28: 325-329, 2006.09.
94. S. J. Oh, Y. H. Yoon, D.I. Kim, Y. Shimasaki, Y. Oshima and T. Honjo, Effects of light quantity and quality on the growth of the harmful dinoflagellate, Cochlodinium polykrikoides Margalef (Dinophyceae)., Algae , 21: 311-316, 2006.08.
95. Inoue S, Oshima Y, Usuki H, Hamaguchi M, Hanamura Y, Kai N, Shimasaki Y, Honjo T, Effects of tributyltin maternal and/or waterborne exposure on the embryonic development of the Manila clam, Ruditapes philippinarum, Chemosphere, 63 881-888, 2006.04.
96. Shimasaki Y, Oshima Y, Inoue S, Inoue Y, Kang I J, Nakayama K, Imoto H and Honjo H, Effect of tributyltin on reproduction in Japanese whiting, Sillago japonica, Marine Environmental Research, 2006.04.
97. Kang IJ, Hano T, Oshima Y, Yokota H, Tsuruda Y, Shimasaki Y, Honjo T, Anti-androgen flutamide affects gonadal development and reproduction in medaka (Oryzias latipes), Marine Environmental Reseach, 2006.04.
98. Sou Nagasoe, Dae-Il Kim, Yohei Shimasaki, Yuji Oshima, Mineo Yamaguchi and Tsuneo Honjo, Effects of temperature, salinity and irradiance on the growth of the red tide dinoflagellate Gyrodinium instriatum Freudenthal et Lee, Harmful Algae, 5(1) 20-25, 2006.04.
99. S. Nagasoe, D.I. Kim, Y. Shimasaki, Y. Oshima, M. Yamaguchi and T. Honjo., Effects of temperature, salinity and irradiance on the growth of the red tide dinoflagellate Gyrodinium instriatum Freudenthal et Lee., Harmful Alga, 10.1016/j.hal.2005.06.001, 5, 1, 20-25, in press, 2005.01.
100. K. Nakayama, Y. Oshima, K. Hiramatsu, Y. Shimasaki and T. Honjo, Effects of polychlorinated biphenyls on the schooling behavior of Japanese medaka, Oryzias latipes., Environmental Toxicology and Chemistry, 10.1897/04-518R2.1, 24, 10, 2588-2593, 24(20):2588-2593., 2005.01.
101. K. Nakayama, Y. Oshima, K. Nagafuchi, T. Hano, Y. Shimasaki, and T. Honjo., Early life stage toxicity in offspring from exposed parent medaka, Oryzias latipes, to mixtures of tributyltin and polychlorinated biphenyls., Environmental Toxicology and Chemistry, 10.1897/04-157R.1, 24, 3, 591-596, 24(3):591-596., 2004.01.
102. Y. Oshima, J. Koyama, K. Nakayama, Y. Inoue, Y. Shimasaki, S. Inoue and T. Honjo., Use of hepatic CYP1A1 activity in Japanese flounder to monitor oil dispersed from the Nakhodka spill., 7(2):123-129., 2004.01.
103. Y. Oshima, T. Takeda, S. Katayama, Y. Inoue, S. Inoue, K. Nakayama, Y. Shimasaki and T. Honjo., Relationship between temperature and pharmacokinetic parameters of florfenicol in carp (Cyprinus carpio)., Japanese Journal of Environmental Toxicology, 7(2): 61-68, 2004.01.
104. M. Omura, Y. Shimasaki, Y. Oshima, K. Nakayama, K. Kubo, S. Aou, R. Ogata, M. Hirata and N. Inoue., Distribution of tributyltin, dibutyltin and monobutyltin in the liver, brain and fat of rats: Two-generation toxicity study of tributyltin chloride., Environmental Sciences, 11(2):123-132., 2004.01.
105. Aou S, Kubo K, Omura M, Oshima Y, Shimasaki Y, Arai O., Effects of bisphenol A and trybutyltin on sexual differentiation of brain and behavior., Journal of Pharmacological Sciences., 91, 35P-35P, 91: 35P-35P Suppl. 1., 2003.01.
106. Y. Oshima, J. Koyama, K. Nakayama, Y. Shimasaki, S. Inoue, Y.Inoue, T. Honjo., Monitoring of dispersed oil pollution by P4501A1 activity in fish., Proceedings of The Second Internationl Work Shop on Bioassay of the Marine Environment in Northwest Pacific Region. Toyama, Japan., pp74-77., 2003.01.
107. Shimasaki Y, Kitano T, Oshima Y, Inoue S, Imada N, Honjo T., Tributyltin causes masculinization in fish., Environmental Toxicology and Chemistry, 10.1897/1551-5028(2003)0222.0.CO;2, 22, 1, 141-144, 22 : 141-144, 2003.01.
108. Y. Shimasaki, Y. Oshima, N. Imada, T. Honjo., Tissue distribution and maternal transfer of butyltins in Japanese whiting, Sillago japonica., Environmental Sciences, 9 (2-3) : 93., 2002.01.
109. M. Omura, Y. Shimasaki, Y. Oshima, K. Nakayama, K. Kubo, S. Aou, R. Ogata,M. Hirata, N. Inoue., Distribution of tributyltin metabolites in the liver and brain of rats - Evaluation in two-generation
toxicity study of tributyltin chloride -., Environmental Sciences, 9 (2-3) : 201., 2002.01.
110. Shimasaki Y, Oshima Y, Yokota Y, Kitano T, Nakao M, Kawabata S-I, Imada N, Honjo T., Purification and identification of a tributyltin-binding protein from serum of Japanese flounder, Paralichthys olivaceus., Environmental Toxicology and Chemistry, 10.1897/1551-5028(2002)0212.0.CO;2, 21, 6, 1229-1235, 21 : 1229-1235, 2002.01.
111. Y. Shimasaki, K. Kitano, Y. Oshima, N. Imada, T. Honjo., Masculinization caused by tributyltin in fish., Environmental Sciences, 8 (2-3) : 126., 2001.01.
112. M. Omura, R. Ogata, K. Kubo, Y. Shimasaki, S. Aou, Y. Oshima, N. Inoue., Tributyltin is a possible aromatase inhibitor in male rat., Environtal Sciences, 8 (2-3) : 263., 2001.01.
113. Omura M, Ogata R, Kubo K, Shimasaki Y, Aou S, Oshima Y, Tanaka A, Hirata M, Makita Y,, Two-generational reproduction toxicity study of tributyltin chloride in male rats., Toxicological Sciences, 10.1093/toxsci/64.2.224, 64, 2, 224-232, 64 : 224-232, 2001.01.
114. Ogata R, Omura M, Shimasaki Y, Kubo K, Oshima Y, Aou S, Inoue N., Two-generational reproduction toxicity study of tributyltin chloride in female rats., Journal of Toxicology and Environ Health Part A, 10.1080/15287390151126469, 63, 2, 127-144, 63 : 127-144, 2001.01.
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