| 1. |
Masanobu Ohishi, Hisaaki Miyahara, Masakazu Kondo, Yasuharu Nakashima, Kazumasa Terada, Yukio Esaki, Nobuo Kobara, Katsumi Harimaya, Yoshihiro Matsumoto, Yukihide Iwamoto, Characteristics of lumbar scoliosis in patients with rheumatoid arthritis, Journal of Orthopaedic Surgery and Research, 10.1186/1749-799X-9-30, 9, 1, 2014.04, Background: Although a substantial percentage of patients with rheumatoid arthritis (RA) experience low back pain, the characteristics of lumbar spine pathology in RA patients has been poorly investigated. In our institutions, lumbar spine radiographs indicated scoliosis in 26 patients. The present study aimed to clarify the characteristics of lumbar scoliosis in RA patients. Methods: This is a retrospective study of 26 RA patients with lumbar scoliosis. Patient characteristics such as disease duration, disease stage and class according to Steinbrocker's classification, and medication for RA and osteoporosis were reviewed. Radiologic evaluation of scoliosis was performed at two different time points by measuring Cobb angles. The progression of scoliosis per year was calculated by dividing the change in Cobb angles by the number of years. Apical vertebral rotation, lateral listhesis, and the level of the intercrestal line at the first observation were also measured. The correlation between different factors and changes in the Cobb angles per year was analyzed. Results: Majority of the patients had a long disease duration and were classified as stage 3 or 4 according to Steinbrocker's classification. During the observation period, most patients were treated with glucocorticoids. Unlike the previous studies on degenerative scoliosis, apical vertebral rotation, lateral listhesis, and the level of the intercrestal line at initial observation were not significantly related to the progression of scoliosis. Initial Cobb angles were inversely related to the progression of scoliosis. Patients who were treated with bisphosphonates showed slower progression of scoliosis. Conclusions: Our results indicate that the characteristics of lumbar scoliosis in RA patients differ from those of degenerative lumbar scoliosis. Bone fragility due to the long disease duration, poor control of disease activity, and osteoporosis is possibly related to its progression.. |
| 2. |
Toshio Doi, Yoshihiro Matsumoto, Osamu Tono, Kiyoshi Tarukado, Katsumi Harimaya, Seiji Okada, Kensuke Kubota, Mitsumasa Hayashida, Yukihide Iwamoto, A shallow chest correlates with the aortic position in the normal spine
Features resembling those observed in structural scoliosis, Scoliosis, 10.1186/1748-7161-9-14, 9, 1, 2014.08, Background: Right thoracic curvature, rib cage deformities and aortic left shift are features of adolescent idiopathic scoliosis that are correlated with each other. We recently reported that disturbance of ribcage development results in progressive thoracic scoliosis in mice. Recently, it has been confirmed that the normal spine exhibits right thoracic curvature and rib cage deformities and that these deformities worsen during the adolescent period. The purpose of this study was to examine whether rib cage deformities correlate with thoracic side curvature in the normal spine, as observed in scoliosis, which is important basic knowledge needed to elucidate the causative factors of adolescent idiopathic scoliosis.Methods: To examine the relationship between rib cage deformities and thoracic side curvature in the normal spine, CT scans of 148 consecutive adult females were examined. The anteroposterior chest dimension, aortic location and rib cage rotation were measured on CT scans obtained at the T8 level. The thoracic side curvature (T5-T12) was also measured on chest radiographs. Results: The anteroposterior chest dimension exhibited a significant correlation with aortic left shift. The aortic location and rib cage rotation were correlated, and the rib cage rotation and thoracic side curvature were correlated. Conclusions: There was a significant correlation between a shallow chest and the aortic position, between the aortic position and the rib cage rotation and between the rib cage rotation and the thoracic side curvature in the normal spine. These findings suggest the possibility that rib cage development is one of the causative factors of adolescent idiopathic scoliosis.. |
| 3. |
Seiji Okada, Takeyuki Saito, Osamu Kawano, Mitsumasa Hayashida, Yoshihiro Matsumoto, Katsumi Harimaya, Yukihide Iwamoto, Sequential changes of ascending myelopathy after spinal cord injury on magnetic resonance imaging
A case report of neurologic deterioration from paraplegia to tetraplegia, Spine Journal, 10.1016/j.spinee.2014.08.449, 14, 12, e9-e14, 2014.12, Background context Marked neurologic deterioration within a few days of traumatic spinal cord injury, known as subacute posttraumatic ascending myelopathy, is rare. Although several hypotheses regarding the pathogenesis of this condition have been proposed, the details remain elusive.
Study design Case report and review of the literature.
Patient sample A 75-year-old woman involved in a motor vehicle collision sustained a fracture dislocation of T7-T8 with complete paraplegia below T8.
Methods Neurologic examination and radiologic imaging taken by various means.
Results Posterior surgical stabilization was performed 18 hours after the injury. Both the surgical and postsurgical courses were uneventful. Four days after the injury, however, the patient reported feeling a tingling sensation in the right-hand fingers and gradually suffered from motor weakness of the upper extremities, deteriorating within a few hours to complete tetraplegia and ventilator dependence. Subsequent cervicothoracic MRI showed abrupt cord swelling with abnormal areas of signal intensity in the cervical and upper thoracic spinal cord during the interval between the onset of tingling and the development of motor paralysis in the arms. On the 20th postsurgical day, an area of hypointensity within the region of high intensity was observed on T2-weighted MRIs, indicating intramedullary spinal cord hemorrhage.
Conclusions Our MRI findings suggest that systemically increased intraspinal pressure resulting from the impairment of spinal venous drainage is involved in the pathogenesis of ascending myelopathy. Although ascending myelopathy is often thought to be partly reversible, persisting increase of the intraspinal pressure may result in intramedullary hemorrhage and irreversible neurologic deficit.. |
| 4. |
A. Nabeshima, Y. Matsumoto, J. Fukushi, K. Iura, T. Matsunobu, M. Endo, T. Fujiwara, K. Iida, Y. Fujiwara, M. Hatano, N. Yokoyama, S. Fukushima, Y. Oda, Y. Iwamoto, Tumour-associated macrophages correlate with poor prognosis in myxoid liposarcoma and promote cell motility and invasion via the HB-EGF-EGFR-PI3K/Akt pathways, British journal of cancer, 10.1038/bjc.2014.637, 112, 3, 547-555, 2015.02, Background:Myxoid liposarcoma (MLS) is the second most common subtype of liposarcoma, and metastasis occurs in up to one-third of cases. However, the mechanisms of invasion and metastasis remain unclear. Tumour-associated macrophages (TAMs) have important roles in tumour invasion, metastasis, and/or poor prognosis. The aim of this study was to investigate the relationship between TAMs and MLS.Methods:Using 78 primary MLS samples, the association between clinical prognosis and macrophage infiltration was evaluated by immunochemistry. The effects of macrophages on cell growth, cell motility, and invasion of MLS cell lines were investigated in vitro. In addition, clinicopathological factors were analysed to assess their prognostic implications in MLS.Results:Higher levels of CD68-positive macrophages were associated with poorer overall survival in MLS samples. Macrophage-conditioned medium enhanced MLS cell motility and invasion by activating epidermal growth factor receptor (EGFR), with the key ligand suggested to be heparin-binding EGF-like growth factor (HB-EGF). The phosphoinositide 3-kinase/Akt pathway was mostly involved in HB-EGF-induced cell motility and invasion of MLS. The expression of phosphorylated EGFR in MLS clinical samples was associated with macrophage infiltration. In addition, more significant macrophage infiltration was associated with poor prognosis even in multivariate analysis.Conclusions:Macrophage infiltration in MLS predicts poor prognosis, and the relationship between TAMs and MLS may be a new candidate for therapeutic targets of MLS.. |
| 5. |
Shuichi Matsuda, Kazuhiro Tanaka, Katsumi Harimaya, Yoshihiro Matsumoto, Hideshi Sato, Yukihide Iwamoto, Treatment of myxoid liposarcoma in pregnancy, Clinical Orthopaedics and Related Research, 10.1097/00003086-200007000-00026, 376, 195-199, 2000.01, Two cases of pregnancy associated with myxoid liposarcoma are presented. Both patients were treated with hyperthermoradiotherapy after the induced delivery of healthy infants and by surgical excision of the tumor 5 to 7 weeks after delivery. No local recurrence or distant metastasis occurred; the children have progressed normally for 4 and 5 years, respectively, after surgery. Because coexistence of pregnancy and sarcoma is rare, it is difficult for the clinician to develop an appropriate approach to the management of bone and soft tissue sarcoma during pregnancy. The literature was reviewed regarding the effect that pregnancy may have on the clinical behavior of sarcoma and the effect treatment for sarcoma may have on the fetus.. |
| 6. |
Yoshinao Oda, Yoshihiro Matsumoto, Katsumi Harimaya, Yukihide Iwamoto, Masazumi Tsuneyoshi, Establishment of new multidrug-resistant human osteosarcoma cell lines, Oncology Reports, 7, 4, 859-866, 2000.01, Multidrug-resistant clones of human osteosarcoma MNNG/HOS and MG63 cells were isolated by stepwise selection on exposure to increasing doses of doxorubicin (DXR). The final clones MNNG/HOS/DXR1000 and MG63/DXR1000, established after ethylmethane sulfonate mutagenesis, showed 96-fold and 121-fold higer resistance to DXR than their parental cell lines. They were also cross-resistant to vincristine, but not to cisplatinum or methotrexate. The levels of multidrug-resistance-1 (MDR1) mRNA expression increased gradually according to the concentration of DXR in both cell lines. Although the parental MNNG/ HOS cells expressed a low level of MDR1 mRNA, the parental MG63 cells showed no MDR1 expression. The IC50 values of MNNG/HOS and its resistant variant to DXR were higher than those of MG63 and its resistant clone. Multidrug-resistant associated protein (MRP) mRNA expression was detected in MNNG/HOS or MG63 parental cell lines, and in their resistant variants. MG63 and its resistant variants revealed stable expression of MRP, whereas the resistant phenotype of MNNG/HOS showed decreased MRP expression, compared to its parental cell line. No alteration in the levels of hepatocyte growth factor (HGF) or its receptor c-MET was recognized between parental lines and their resistant variants. The results indicate that our DXR-resistant variants of MNNG/HOS and MG63 reveal a classical MDR phenotype and can offer a model with which to investigate the mechanisms of multidrug resistance in osteosarcoma.. |
| 7. |
Katsumi Harimaya, K. Tanaka, Yoshihiro Matsumoto, H. Sato, S. Matsuda, Y. Iwamoto, Antioxidants inhibit TNFα-induced motility and invasion of human osteosarcoma cells
Possible involvement of NFκB activation, Clinical and Experimental Metastasis, 10.1023/A:1006791723233, 18, 2, 121-129, 2000.12, Osteosarcoma is the most frequent malignant bone tumor in children. It is highly invasive, however, the mechanisms behind osteosarcoma cell invasion are as yet still unknown. In the present study, treatment with TNFα enhanced the invasiveness of two human osteosarcoma cell lines, OST and MNNG. TNFα treatment also induced tumor cell motility, adhesion to laminin, the expression of matrix metalloproteinase 9 (MMP9), and the nuclear translocation of nuclear factor κB (NFκB) in the osteosarcoma cells. Moreover, antioxidants inhibited TNFα-induced osteosarcoma cell invasion, motility and NFκB nuclear translocation, but not adhesion to laminin or MMP9 expression. NFκB decoy, another NFκB inhibitor, also inhibited TNFα-induced osteosarcoma cell invasion and motility. Therefore, motility and NFκB activation were possibly related to TNFα-induced osteosarcoma cell invasion. However, adhesion to laminin or MMP did not demonstrate any correlation with TNFα-induced osteosarcoma cell invasion. Although NFκB is known to regulate TNFα-induced phenotypes, it may influence only motility and invasion, but not the MMP or laminin-mediated adhesion of these osteosarcoma cells.. |
| 8. |
Yoshihiro Matsumoto, K. Tanaka, Katsumi Harimaya, F. Nakatani, S. Matsuda, Y. Iwamoto, Small GTP-binding protein, Rho, both increased and decreased cellular motility, activation of matrix metalloproteinase 2 and invasion of human osteosarcoma cells, Japanese Journal of Cancer Research, 10.1111/j.1349-7006.2001.tb01113.x, 92, 4, 429-438, 2001.01, Rho, a member of the small GTP-binding proteins, and one of its downstream effectors ROCK (Rho-associated coiled-coil forming protein kinase) play an important role in the invasion of tumor cells. Lysophosphatidic acid (LPA) activates Rho and ROCK and promotes the organization of stress fibers and focal adhesions. However, the effect of LPA on tumor cell invasion is still controversial. In the present study, human osteosarcoma cells treated with a high concentration of LPA (high LPA) showed considerable formation of stress fibers and focal adhesions compared to the cells treated with a low concentration of LPA (low LPA). C3 (inhibitor of Rho) or Y27632 (an inhibitor of ROCK) inhibited the effects of LPA, indicating that LPA activates the Rho-ROCK pathway in the cells. In addition, Rho activation assay showed that the activation level of Rho can be altered by changing the concentration of LPA. Low LPA stimulated the motility and invasion of the cells, while high LPA reduced both. The disruption of extracellular matrix (ECM) by matrix metalloproteinase 2 (MMP2) is also critical for tumor cell invasion. MMP2 is activated by membranous type-1 MMP (MT1-MMP) and type-2 tissue inhibitor of MMP (TIMP2). High LPA suppressed the activation of MMP2 through down-regulation of MT1-MMP and TIMP2. C3 and Y27632 reversed the suppression of the activation of MMP2 and expression of MT1-MMP and TIMP2, suggesting the involvement of the Rho-ROCK pathway in ECM degradation. Tyrosine phosphorylation of focal adhesion kinase (FAK) was also required for the invasion of tumor cells to occur. Low LPA enhanced the tyrosine phosphorylation of FAK whereas high LPA reduced it. In conclusion, we suggest that Rho has a dual effect on the invasion of osteosarcoma cells by modulating the motility, the ability to degrade ECM and tyrosine phosphorylation of FAK.. |
| 9. |
Fukushima S, Matsumoto Y, Endo M, Matsumoto Y, Fukushi JI, Matsunobu T, Kawaguchi KI, Setsu N, IIda K, Yokoyama N, Nakagawa M, Yahiro K, Oda Y, Iwamoto Y, Nakashima Y., Hypoxia-inducible Factor 1 alpha is a Poor Prognostic Factor and Potential Therapeutic Target in Malignant Peripheral Nerve Sheath Tumor. , PLOS One, 12, e0178064-e0178064, 2017.06. |
| 10. |
Yokoyama N, Matsunobu T, Matsumoto Y, Fukushi J, Endo M, Hatano M, Nabeshima A, Fukushima S, Okada S, and Iwamoto Y., Pazopanib Resistance via Downregulation of DUSP6 in Synovial Sarcoma Cells., Scientific reports, 7, 45332-45332, 2017.06. |
| 11. |
Matsumoto Y, Harimaya K, Kawaguchi K, Hayashida M, Okada S, Doi T, Iwamoto Y., Dumbbell Scoring System: A New Method for the Differential Diagnosis of Malignant and Benign Spinal Dumbbell Tumors., Spine, 41, E1230-E1236, 2016.06. |
| 12. |
Ueda T, Morioka H, Nishida Y, Kakunaga S, Tsuchiya H, Matsumoto Y, Asami Y, Inoue T, Yoneda T., Objective tumor response to denosumab in patients with giant cell tumor of bone: a multicenter phase II trial. , Ann Oncology., 26, 2149-2154, 2015.06. |
| 13. |
Hatano M, Matsumoto Y, Fukushi J, Matsunobu T, Endo M, Okada S, Iura K, KamuraS, Fujiwara T, Iida K, Fujiwara Y, Nabeshima A, Yokoyama N, Fukushima S, Oda Y, Iwamoto Y., Cadherin-11 regulates the metastasis of Ewing sarcoma cells to bone. Clin Exp Metastasis., Clin Exp Metastasis, 32, 579-591, 2015.06. |
| 14. |
Matsumoto Y, Matsumoto K, Harimaya K, Okada S, Doi T, Iwamoto Y., Scoliosis in patients with multiple hereditary exostoses., Eur Spine J, 24, 1568-1573, 2015.06. |
| 15. |
Yoshihiro Matsumoto, Malignant peripheral nerve sheath tumors presenting as spinal dumbbell tumors: clinical outcomes and characteristic imaging features., 2014.09. |
| 16. |
Yoshihiro Matsumoto, Imaging analysis of lumbar intervertebral disc degeneration in professional baseball players. , 5, 855-860, 2014.04. |
| 17. |
Yoshihiro Matsumoto, Matsunobu T, Endo M, Oda Y, Iwamoto Y, Intraosseous hemangioma arising in the clavicle., 43, 89-93, 2013.10. |
| 18. |
Yoshihiro Matsumoto, Harimaya K, Hayashida M, Okada S, Iwamoto Y, Minimum five-year follow-up of selective anterior thoracolumbar or lumbar fusion for adolescent idiopathic scoliosis: an analysis of radiographic parameters relevant to the uppermost instrumented vertebra and postoperative spinal balance., 4, 933-940, 2013.07. |
| 19. |
Kubota K, Doi T, Matsumoto y, Okada S, Iwamoto Y, Disturbance of Rib Cage Development Causes
Progressive Thoracic Scoliosis: The Creation of a Nonsurgical Structural
Scoliosis Model in Mice.
, 95, e1301-e1307, 2013.07. |
| 20. |
Yoshihiro Matsumoto, Fujiwara-Okada Y, Iwamoto Y, Oda Y, Y-box binding protein-1 regulates cell proliferation and is associated with
clinical outcomes of osteosarcoma.
, 108, 836-847, 2013.05. |
| 21. |
Yoshihiro Matsumoto, Katsumi Harimaya, Yukihide Iwamoto, Yoshinao Oda, Hayashida Mitsumasa, Dedifferentiated
chondrosarcoma of the cervical spine: a case report.
, World J Surg Oncol., 11, 1, 32-32, 2013.01. |
| 22. |
Endo M, Yamamoto H, Setsu N, Kohashi K, Takahashi Y, Ishii T, Iida K, Matsumoto Y, Yukihide Iwamoto, Yoshinao Oda, Prognostic significance of AKT/mTOR and MAPK pathways and antitumor effect
of mTOR inhibitor in NF1-related and sporadic malignant peripheral nerve sheath
tumors., Clin Cancer Res., 19, 2, 450-461, 2013.01. |
| 23. |
Yoshihiro Matsumoto, Kazuya Yokota, Akio Sakamoto, Yukihide Iwamoto, Yoshinao Oda, Clinical outcome for patients with dedifferentiated chondrosarcoma: a report of 9
cases at a single institute.
, 7, 1, 32-32, On line journal, 2012.12. |
| 24. |
Kubota K, Saiwai H, Kumamaru H, Kobayakawa K, Maeda T, Matsumoto Y, Harimaya K, Iwamoto Y, Okada S, Neurological recovery is impaired by concurrent but not by
asymptomatic pre-existing spinal cord compression after traumatic spinal cord
injury. , Spine, 37, 17, 1448-1455, 2012.08. |
| 25. |
Yoshihiro Matsumoto, Li Y, Li X, Fukushi JI, Iwamoto Y, Impairment of
p53 acetylation by EWS-Fli1 chimeric protein in Ewing Family Tumors.
, Cancer Letter, 320, 1, 14-22, 2012.07. |
| 26. |
Yoshihiro Matsumoto, Matsuda S, Harimaya K, Sakamoto A, Clinical results of open synovectomy for treatment of diffuse pigmented villonodular synovitis of the knee: case series and review of literature, The Knee, 2012.01. |
| 27. |
Matsumoto Y, Harimaya K, Doi T, Kawaguchi K, Okada S, Inoguchi A, Fujiwara M, Iwamoto Y, Clinical characteristics and surgical outcome of the symptomatic ossification of ligamentum flavum at the thoracic level with combined lumbar spinal stenosis, Arch Orthop Trauma Surg, 132, 465-470, 2011.11. |
| 28. |
Fujiwara T, Fukushi J, Yamamoto S, Matsumoto Y, Setsu N, Oda Y, Yamada H, Okada S, Watari K, Ono M, Kuwano M, Kamura S, Iida K, Okada Y, Koga M, Iwamoto Y, Macrophage infiltration predicts a poor prognosis for human ewing sarcoma, Am J Pathol, 179, 1157-1170, 2011.08. |
| 29. |
Yamaguchi Y, Inatani M, Matsumoto Y, Ogawa J, Irie F, Roles of heparan sulfate
in mammalian brain development current views based on the findings from Ext1
conditional knockout studies, Prog Mol Biol Transl Sci, 93, 1, 133-152, 2011.05. |
| 30. |
Doi T, Kido S, Kuwashima U, Tono O, Tarukado K, Harimaya K, Matsumoto Y,
Iwamoto Y
, A new method for measuring torsional deformity in scoliosis, Scoliosis, 6, on line journal, issue 7, 2011.05. |
| 31. |
Doi T, Harimaya K, Matsumoto Y, Tono O, Tarukado K, Iwamoto Y, Endoscopic
decompression for intraforaminal and extraforaminal nerve root compression
, J Orthop Surg Res
, 6, on line journal, issue 16, 2011.05. |
| 32. |
Matsumoto Y , Yuko Okada , Jun-ichi Fukushi , Satoshi Kamura , Toshifumi Fujiwara , Keiichiro Iida , Mihoko Koga , Shuichi Matsuda , Katsumi Harimaya , Akio Sakamoto and Yukihide Iwamoto. , Role of the VEGF-Flt-1-FAK pathway in the pathogenesis of osteoclastic bone destruction of giant cell tumors of bone , Journal of Orthopaedic Surgery and Research, 5, 1, 85, 2010.10. |
| 33. |
Kamura S, Matsumoto Y, Fukushi JI, Fujiwara T, Iida K, Okada Y, Iwamoto Y, Basic fibroblast growth factor in the bone microenvironment enhances cell motility and invasion of Ewing's sarcoma family of tumours by activating the FGFR1-PI3K-Rac1 pathway, Br. J. Cancer, 103, 3, 370-381, 2010.08. |
| 34. |
Matsumoto Y, Matsumoto K, Irie F, Fukushi J, Stallcup WB, Yamaguchi Y, Conditional ablation of the heparan sulfate-synthesizing enzyme Ext1 leads to dysregulation of bone morphogenic protein signaling and severe skeletal defects. , J. Bio. Chem., 285, 25, 19227-19234, 2010.05. |
| 35. |
Matsumoto Y, Harimaya K, Doi T, Maeda T, Iwamoto Y. , Outcome of osteoplastic laminotomy for excision of spinal cord tumors., J Orthop Surg. , 17, 275-279, , 2009.10. |
| 36. |
Okada S, Maeda T, Ohkawa Y, Harimaya K, Saiwai H, Kumamaru H, Matsumoto Y, Doi T, Ueta T, Shiba K, Iwamoto Y., Does ossification of the posterior longitudinal ligament affect the neurological outcome after traumatic cervical cord injury? , Spine, 34, 1148-1152, 2009.07. |
| 37. |
Iwao K, Inatani M, Matsumoto Y, Ogata-Iwao M, Takihara Y, Irie F, Yamaguchi Y, Okinami S, Tanihara H. , Heparan sulfate deficiency leads to Peters anomaly in mice by disturbing neural crest TGF-beta2 signaling, J Clin Invest., 119, 1997-2008, 2009.05. |
| 38. |
Yoshihiro Matsumoto, Shuichi Matsuda, Koji Matono, Yoshinao Oda, Masazumi Tsuneoshi, Yukihide Iwamoto, Intra-Articular Osteochondroma of the Knee Joint in a patient with Hereditary Multiple Osteochondromatosis, Fukuoka Acta Medica, 98: 425-430, 2007.12. |
| 39. |
Kadoya K, Fukushi JI, Matsumoto Y, Yamaguchi Y, Stallcup WB.,
NG2 Proteoglycan Expression in Mouse Skin: Altered Postnatal Skin Development in the NG2 Null Mouse. , J Histochem Cytochem. 2007 , 2007.05. |
| 40. |
Yoshihiro Matsumoto, Fumitoshi Irie, Masaru Inatani, Marc Tessier-Lavigne, Yu Yamaguchi, Netrin-1/DCC Signaling in Commissural Axon Guidance Requires Cell-autonomous Expression of Heparan Sulfate, Journal of Neuroscience, 27: 4342-4350, 2007.04. |
