| 1. |
Shuji Fujimoto, Fumiko Kojima, Efficient isolation of therapeutic drug-resistant Campylobacter., 17th Asia Pacific Congress of Clinical Microbiology & Infection, 2018.08. |
| 2. |
Shuji Fujimoto, Fumiko Kojima, Efficient Isolation of Therapeutic Erythromycin-Resistant Campylobacter., International Meeting on Emerging Diseases and Surveillance 2018, 2018.11. |
| 3. |
Fumiko Kojima, Shuji Fujimoto, In Vitro Penetrability of Anisakis sinplex Larvae., International Meeting on Emerging Diseases and Surveillance 2018, 2018.11, Anisakis larvae were isolated from Scomber japonics fish those were caught in the sea around Goto Islands (Nagasaki Prefecture, Japan), the sea around Kanagawa Prefecture (Japan) and the sea around Jeju Island (Korea). We used Anisakis simplex larvae those were identified by morphological features in the study. For identification of the sibling species, PCR-RFLP for ribosomal DNA internal transcribed spacer (rDNA ITS) regions was performed. The penetrability of the larvae were evaluated with the agar method which was previously reported1). We examined 138 A. simplex larvae (third stage) for comparison of the penetrability among the species (A. pegreffii, A. simplex sensu stricto). The penetration rate of A. pegreffii and A. simplex s. s. was 79 and 68%, respectiively. A. pegreffii showed rapid penetrability in comparison with A. simplex s. s.. Penetrability under the long term storage in saline at 4℃ was also examined. A. simplex larvae survived for over 14 weeks. The penetrability was kept for long times as long as they lives. The larvae penetrated more slowly in proportion to the storage time. The results showed that our method is useful to test the penetrability of Anisakid larvae. Both A. pegreffii and A. simplex s. s. larvae had high ability to penetrate into agar, but the former penetrated quickly than the latter. In addition, larvae's penetrability was kept even be an imperfect body and by the storage under the cold condition. Further studies should be done to clear the reasons why almost of anisakiasis iscaused by A. simplex s. s. larvae.
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| 4. |
Genomic identification based on PCR-RAPD in Anisakis simplex larvae.. |
| 5. |
Shuji Fujimoto, Fumiko Kojima, Studies of Photocatalytic Microbicidal Effect of Titaninm Dioxide(TIO2) Nanoparticles on Pathogenic Microorganisms., International Union of Microbiological Societies 2017, 2017.07. |
| 6. |
Fumiko Kojima, Shuji Fujimoto, Genomic identification of Anisakis simplex larvae by PCR-RAPD., International Union of Microbiological Societies 2017, 2017.07, Anisakiasis is a disease caused by infection with an anisakid larvae, mostly Anisakis simplex. The lavaes commonly infect in marine fish and the disease is frequently reported in areas of the world where fish is consumed raw. In Japan, people have the habit of eating raw fish, so they have more chance of infection with anisakid larvae.
There are three sibling species in A. simplex larvae, namely, A. simplex sensu stricto (Asss), A. pegreffii (Ap) and A. simplex C. It was revealed that Ap is dominant among the larvae from fish in the Japan Sea side and Asss. is dominant among those of the Pacific Ocean side conversely. Although anisakiasis is happened anywhere in Japan, several studies reported that the isolates from the patients with anisakiasis were Asss. exclusively. It suggests that there might be differences in the virulence of the sibling species. In order to investigate the genetic difference among the each A. simplex larvae, we used RAPD technique to differentiate individuals of A. simplex obtained from fish those were caught on both coast. The RAPD patterns of the control DNAs were markedly different from those of the A. simplex. There were differences in amplification patterns between Asss and Ap. The RAPD patterns for larvae obtained from fish of the same sea were somewhat different and variations were detected even among larvae from the same fish. These results suggest the considerable high genetic variability between Ass and Ap and the possible existence of genetic variation within the sibling species.. |
| 7. |
Shuji Fujimoto, Fumiko Kojima, Mika Shigematsu, Biorisk management education for undergraduates studying clinical microbiology at university in Japan., 16th Asia Pacific Congress of Clinical Microbiology & Infection, 2016.12, Biorisk management ( Biosafety/Biosecurity ) is required for anyone working in a clinical laboratory where infectious agents and potentially hazardous biological materials are examined/stored. Proper education and training based on international standards of biorisk management should be provided not only as a part of laboratory safety program in work place but also as a part of introductory training at educational institutions for continuity and to elevate overall baseline of the biorisk management. We reported results of the pilot study of biorisk management education for graduate students majored in laboratory diagnostics previously. However, postgraduate education is still late in their profession and the participants’ interview also revealed importance and demands of earlier biorisk management education for undergraduates. Create programme for systematic education and training of biosafety and biosecurity for students employing many different teaching approaches to make importance of risk management unforgettable and understandable in their fresh mind.. |
| 8. |
Fujimoto Shuji, Shigematsu Mika, Kojima Fumiko, Alternative to dry ice: examination of temperature control with cold-storage plate system during hot summer transportation., 57th Annual Biological Safety Conference, 2014., 2014.10. |
| 9. |
Fumiko Kojima, Shuji Fujimoto, Genomic identification based on PCR-RAPD in Anisakis simplex larvae., 16th Asia Pacific Congress of Clinical Microbiology & Infection, 2016.12, Anisakiasis is a disease caused by infection with an anisakid larvae, mostly Anisakis simplex. The lavaes commonly infect in marine fish and the disease is frequently reported in areas of the world where fish is consumed raw. In Japan, people have the habit of eating raw fish, so they have more chance of infection with anisakid larvae.
There are three sibling species in A. simplex larvae, namely, A. simplex sensu stricto (Asss), A. pegreffii (Ap) and A. simplex C. It was revealed that Ap is dominant among the larvae from fish in the Japan Sea side and Asss. is dominant among those of the Pacific Ocean side conversely. Although anisakiasis is happened anywhere in Japan, several studies reported that the isolates from the patients with anisakiasis were Asss. exclusively. It suggests that there might be differences in the virulence of the sibling species. In order to investigate the genetic difference among the each A. simplex larvae, we used RAPD technique to differentiate individuals of A. simplex obtained from fish those were caught on both coast. The RAPD patterns of the control DNAs were markedly different from those of the A. simplex. There were differences in amplification patterns between Asss and Ap. The RAPD patterns for larvae obtained from fish of the same sea were somewhat different and variations were detected even among larvae from the same fish. These results suggest the considerable high genetic variability between Ass and Ap and the possible existence of genetic variation within the sibling species.
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| 10. |
Fukuda Hiroyuki, Kojima Fumiko, Fujimoto Shuji, Discrimination of Campylobacter species by a degenerate PCR-RFLP method based on gyrB gene sequence., 18th International Workshop on Campylobacter, Helicobacter and Related Organisms (CHRO) Conference 2015., 2015.11. |
| 11. |
Analysis of Anisakis simplex larvae isolated from human.. |
| 12. |
Fumiko Kojima, Shuji Fujimoto, Genomic identification of Anisakis simplex larvae by PCR-RAPD., International Meeting on Emerging Diseases and Surveillance, 2014.11, Anisakiasis is a disease caused by infection with an anisakid larvae, mostly Anisakis simplex. The lavaes commonly infect in marine fish and the disease is frequently reported in areas of the world where fish is consumed raw, lightly pickled or salted. In Japan, people have the habit of eating raw fish such as ‘sushi’ or ‘sashimi’, so they have more chance of infection with larvae of anisakid nematodes.
There are three sibling species in A. simplex larvae, namely, A. simplex sensu stricto (Asss), A. pegreffii (Ap) and A. simplex C. It was revealed that Ap is dominant among the larvae from fish (Scomber japonics ) in the Japan Sea side and Asss. is dominant among those of the Pacific Ocean side conversely. Although anisakiasis is happened in Japan among both the Japan Sea side area and the Pacific Ocean side area, several studies reported that the isolates from the patients with anisakiasis were Asss. exclusively. It suggests that there might be differences in the virulence of the sibling species. The aim of this study was to investigate genetic variations between the siblings (Asss and Ap) and within the same sibling specie by random amplified polymorphic DNA (RAPD) technique..
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| 13. |
Penetrability of Anisakis Larvae under storage at 4℃.. |
| 14. |
Penetrability of Anisakis simplex larvae with agar. |
| 15. |
Shigematsu Mika, Fujimoto Shuji, Kojima Fumiko, Biorisk Management Initiation Training for Graduate and Undergraduate Medical and Co-medical Students., 56th Annual Biological Safety Conference, 2013., 2013.10. |
| 16. |
小島 夫美子, 藤本 秀士, Evaluation of Anisakis simplex larvae's penetrability using agar method., 28th International Congress of Chemotherapy and Infection, 2013.06. |
| 17. |
藤本 秀士, 重松美加, 小島 夫美子, Molecular epidemiological analysis on Campylobacter isolates associated with food poisoning., 28th International Congress of Chemotherapy and Infection, 2013.06. |
| 18. |
藤本 秀士, 小島 夫美子, 重松美加, Molecular biological analysis Campylobacter isolates associated with food poisoning, 日本分子生物学会, 2012.12. |
| 19. |
Penetrability of Anisakis simplex larvae with agar. |
| 20. |
Negative Stain is Useful for The Rapid Detection of Helminth Eggs in Clinical Fecal Specimens. |
| 21. |
Evaluating Method of the Penetration Capacity of Anisakis Larvae with an Agar.. |
| 22. |
The advantage of the electronic parasite-eggs atlas for the education in Parasitology.. |
| 23. |
Negative stain is useful in rapid detection of helminth eggs.. |
| 24. |
The morphological and genetic study of Anisakis larvae which found in mackerel fished in the same sea area.. |
