Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Takeshi Ishihara Last modified date:2021.10.22

Professor / Informational Biology / Department of Biology / Faculty of Sciences

1. Hino T, Hirai S, Ishihara T, Fujiwara M., EGL-4/PKG regulates the role of an interneuron in a chemotaxis circuit of C. elegans through mediating integration of sensory signals, GENES TO CELLS, 10.1111/gtc.12849, 26, 6, 411-425, 2021.06.
2. Wen C, Miura T, Voleti V, Yamaguchi K, Tsutsumi M, Yamamoto K, Otomo K, Fujie Y, Teramoto T, Ishihara T, Aoki K, Nemoto T, Hillman EM, Kimura KD., 3DeeCellTracker, a deep learning-based pipeline for segmenting and tracking cells in 3D time lapse images, ELIFE, 10.7554/eLife.59187, 10, 2021.03.
3. Harada, Kazuki; Chihara, Takami; Hayasaka, Yuki; Mita, Marie; Takizawa, Mai; Ishida, Kentaro; Arai, Mary; Tsuno, Saki; Matsumoto, Mitsuharu; Ishihara, Takeshi; Ueda, Hiroshi; Kitaguchi, Tetsuya; Tsuboi, Takashi, Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging, SCIENTIFIC REPORTS, 10.1038/s41598-020-76440-4, 10, 1, 2020.11.
4. Yu Toyoshima, Stephen Wu, Manami Kanamori, Hirofumi Sato, Moon Sun Jang, Suzu Oe, Yuko Murakami, Takayuki Teramoto, Chanhyun Park, Yuishi Iwasaki, Takeshi Ishihara, Ryo Yoshida, Yuichi Iino, Neuron ID dataset facilitates neuronal annotation for whole-brain activity imaging of C. elegans, BMC biology, 10.1186/s12915-020-0745-2, 18, 1, 2020.03, Background: Annotation of cell identity is an essential process in neuroscience that allows comparison of cells, including that of neural activities across different animals. In Caenorhabditis elegans, although unique identities have been assigned to all neurons, the number of annotatable neurons in an intact animal has been limited due to the lack of quantitative information on the location and identity of neurons. Results: Here, we present a dataset that facilitates the annotation of neuronal identities, and demonstrate its application in a comprehensive analysis of whole-brain imaging. We systematically identified neurons in the head region of 311 adult worms using 35 cell-specific promoters and created a dataset of the expression patterns and the positions of the neurons. We found large positional variations that illustrated the difficulty of the annotation task. We investigated multiple combinations of cell-specific promoters driving distinct fluorescence and generated optimal strains for the annotation of most head neurons in an animal. We also developed an automatic annotation method with human interaction functionality that facilitates annotations needed for whole-brain imaging. Conclusion: Our neuron ID dataset and optimal fluorescent strains enable the annotation of most neurons in the head region of adult C. elegans, both in full-automated fashion and a semi-automated version that includes human interaction functionalities. Our method can potentially be applied to model species used in research other than C. elegans, where the number of available cell-type-specific promoters and their variety will be an important consideration..
5. Yoshishige Kimura, Koji Tsutsumi, Alu Konno, Koji Ikegami, Saira Hameed, Tomomi Kaneko, Oktay Ismail Kaplan, Takayuki Teramoto, Manabi Fujiwara, Takeshi Ishihara, Oliver E. Blacque, Mitsutoshi Setou, Environmental responsiveness of tubulin glutamylation in sensory cilia is regulated by the p38 MAPK pathway, Scientific reports, 10.1038/s41598-018-26694-w, 8, 1, 2018.12, Glutamylation is a post-translational modification found on tubulin that can alter the interaction between microtubules (MTs) and associated proteins. The molecular mechanisms regulating tubulin glutamylation in response to the environment are not well understood. Here, we show that in the sensory cilia of Caenorhabditis elegans, tubulin glutamylation is upregulated in response to various signals such as temperature, osmolality, and dietary conditions. Similarly, tubulin glutamylation is modified in mammalian photoreceptor cells following light adaptation. A tubulin glutamate ligase gene ttll-4, which is essential for tubulin glutamylation of axonemal MTs in sensory cilia, is activated by p38 MAPK. Amino acid substitution of TTLL-4 has revealed that a Thr residue (a putative MAPK-phosphorylation site) is required for enhancement of tubulin glutamylation. Intraflagellar transport (IFT), a bidirectional trafficking system specifically observed along axonemal MTs, is required for the formation, maintenance, and function of sensory cilia. Measurement of the velocity of IFT particles revealed that starvation accelerates IFT, which was also dependent on the Thr residue of TTLL-4. Similarly, starvation-induced attenuation of avoidance behaviour from high osmolality conditions was also dependent on ttll-4. Our data suggest that a novel evolutionarily conserved regulatory system exists for tubulin glutamylation in sensory cilia in response to the environment..
6. Hirose, Osamu; Kawaguchi, Shotaro; Tokunaga, Terumasa; Toyoshima, Yu; Teramoto, Takayuki; Kuge, Sayuri; Ishihara, Takeshi; Iino, Yuichi; Yoshida, Ryo, SPF-CellTracker: Tracking Multiple Cells with Strongly-Correlated Moves Using a Spatial Particle Filter, IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS, 10.1109/TCBB.2017.2782255, 15, 6, 1822-1831, 2018.11.
7. Sayuri Hara-Kuge, Tomonobu Nishihara, Tomoki Matsuda, Tomohiro Kitazono, Takayuki Teramoto, Takeharu Nagai, Takeshi Ishihara, An improved inverse-type Ca2+ indicator can detect putative neuronal inhibition in Caenorhabditis elegans by increasing signal intensity upon Ca2+ decrease, PLoS One, 10.1371/journal.pone.0194707, 13, 4, 2018.04, Sensory processing is regulated by the coordinated excitation and inhibition of neurons in neuronal circuits. The analysis of neuronal activities has greatly benefited from the recent development of genetically encoded Ca2+ indicators (GECIs). These molecules change their fluorescence intensities or colours in response to changing levels of Ca2+ and can, therefore, be used to sensitively monitor intracellular Ca2+ concentration, which enables the detection of neuronal excitation, including action potentials. These GECIs were developed to monitor increases in Ca2+ concentration; therefore, neuronal inhibition cannot be sensitively detected by these GECIs. To overcome this difficulty, we hypothesised that an inverse-type of GECI, whose fluorescence intensity increases as Ca2+ levels decrease, could sensitively monitor reducing intracellular Ca2+ concentrations. We, therefore, developed a Ca2+ indicator named inverse-pericam 2.0 (IP2.0) whose fluorescent intensity decreases 25-fold upon Ca2+ binding in vitro. Using IP2.0, we successfully detected putative neuronal inhibition by monitoring the decrease in intracellular Ca2+ concentration in AWCON and ASEL neurons in Caenorhabditis elegans. Therefore, IP2.0 is a useful tool for studying neuronal inhibition and for the detailed analysis of neuronal activities in vivo..
8. Osamu Hirose, Shotaro Kawaguchi, Terumasa Tokunaga, Yu Toyoshima, Takayuki Teramoto, Sayuri Kuge, Takeshi Ishihara, Yuichi Iino, Ryo Yoshida, SPF-CellTracker
Tracking multiple cells with strongly-correlated moves using a spatial particle filter, IEEE/ACM Transactions on Computational Biology and Bioinformatics, 10.1109/TCBB.2017.2782255, 2017.12, Tracking many cells in time-lapse 3D image sequences is an important challenging task of bioimage informatics. Motivated by a study of brain-wide 4D imaging of neural activity in C. elegans, we present a new method of multi-cell tracking. Data types to which the method is applicable are characterized as follows: (i) cells are imaged as globular-like objects, (ii) it is difficult to distinguish cells based only on shape and size, (iii) the number of imaged cells ranges in several hundreds, (iv) moves of nearly-located cells are strongly correlated and (v) cells do not divide. We developed a tracking software suite which we call SPF-CellTracker. Incorporating dependency on cells moves into prediction model is the key to reduce the tracking errors: cell-switching and coalescence of tracked positions. We model target cells correlated moves as a Markov random field and we also derive a fast computation algorithm, which we call spatial particle filter. With the live-imaging data of nuclei of C. elegans neurons in which approximately 120 nuclei of neurons are imaged, we demonstrate an advantage of the proposed method over the standard particle filter and a method developed by Tokunaga et al. (2014)..
9. Tomohiro Kitazono, Sayuri Hara-Kuge, Osamu Matsuda, Akitoshi Inoue, Manabi Fujiwara, Takeshi Ishihara, Multiple signaling pathways coordinately regulate forgetting of olfactory adaptation through control of sensory responses in caenorhabditis elegans, Journal of Neuroscience, 10.1523/JNEUROSCI.0031-17.2017, 37, 42, 10240-10251, 2017.10, Forgetting memories is important for animals to properly respond to continuously changing environments. To elucidate the mechanisms of forgetting, we used one of the behavioral plasticities of Caenorhabditis elegans hermaphrodite, olfactory adaptation to an attractive odorant, diacetyl, as a simple model of learning. In C. elegans, the TIR-1/JNK-1 pathway accelerates forgetting of olfactory adaptation by facilitating neural secretion from AWC sensory neurons. In this study, to identify the downstream effectors of the TIR-1/JNK-1 pathway, we conducted a genetic screen for suppressors of the gain-of-function mutant of tir-1 (ok1052), which shows excessive forgetting. Our screening showed that three proteins—a membrane protein, MACO-1; a receptor tyrosine kinase, SCD-2; and its putative ligand, HEN-1—regulated forgetting downstream of the TIR-1/JNK-1 pathway. We further demonstrated that MACO-1 and SCD-2/HEN-1 functioned in parallel genetic pathways, and only MACO-1 regulated forgetting of olfactory adaptation to isoamyl alcohol, which is an attractive odorant sensed by different types of sensory neurons. In olfactory adaptation, odor-evoked Ca2+ responses in olfactory neurons are attenuated by conditioning and recovered thereafter. A Ca2+ imaging study revealed that this attenuation is sustained longer in maco-1 and scd-2 mutant animals than in wild-type animals like the TIR-1/JNK-1 pathway mutants. Furthermore, temporal silencing by histamine-gated chloride channels revealed that the neuronal activity of AWC neurons after conditioning is important for proper forgetting. We propose that distinct signaling pathways, each of which has a specific function, may coordinately and temporally regulate forgetting by controlling sensory responses..
10. Yu Toyoshima, Terumasa Tokunaga, Osamu Hirose, Manami Kanamori, Takayuki Teramoto, Moon Sun Jang, Sayuri Kuge, Takeshi Ishihara, Ryo Yoshida, Yuichi Iino, Accurate Automatic Detection of Densely Distributed Cell Nuclei in 3D Space, PLoS Computational Biology, 10.1371/journal.pcbi.1004970, 12, 6, 2016.06, To measure the activity of neurons using whole-brain activity imaging, precise detection of each neuron or its nucleus is required. In the head region of the nematode C. elegans, the neuronal cell bodies are distributed densely in three-dimensional (3D) space. However, no existing computational methods of image analysis can separate them with sufficient accuracy. Here we propose a highly accurate segmentation method based on the curvatures of the iso-intensity surfaces. To obtain accurate positions of nuclei, we also developed a new procedure for least squares fitting with a Gaussian mixture model. Combining these methods enables accurate detection of densely distributed cell nuclei in a 3D space. The proposed method was implemented as a graphical user interface program that allows visualization and correction of the results of automatic detection. Additionally, the proposed method was applied to time-lapse 3D calcium imaging data, and most of the nuclei in the images were successfully tracked and measured..
11. Manabi Fujiwara, Itaru Aoyama, Takahiro Hino, Takayuki Teramoto, Takeshi Ishihara, Gonadal Maturation Changes Chemotaxis Behavior and Neural Processing in the Olfactory Circuit of Caenorhabditis elegans, Current Biology, 10.1016/j.cub.2016.04.058, 26, 12, 1522-1531, 2016.01, Many animal species change their behavior according to their stage of development. However, the mechanisms involved in translating their developmental stage into the modifications of the neuronal circuits that underlie these behavioral changes remain unknown. Here we show that Caenorhabditis elegans changes its olfactory preferences during development. Larvae exhibit a weak chemotactic response to the food-associated odor diacetyl, whereas adults exhibit a strong response. We show that germline loss, caused either by laser ablation of germline precursor cells or mutations, results in a diacetyl-specific chemotactic defect in adult animals. These results suggest that germline cells, which proliferate dramatically during the larval stages, enhance chemotaxis to diacetyl. Removal experiments of specific neurons suggested that AWA olfactory neurons and their downstream interneurons, AIA and AIB, are required for germline-dependent chemotactic enhancement. Calcium imaging in animals lacking germline cells indicates that the neural responses of AWA and AIB to diacetyl stimuli are decreased compared with animals with an intact germline. These changes in neural activities may at least partly explain the behavioral change of animals lacking germline cells. Furthermore, this germline-dependent chemotactic change depends on the transcription factor DAF-16/FOXO. We find that organismal behavior changes throughout development by integrating information about physiological status from internal tissues to modify a simple sensory circuit..
12. Manabi Fujiwara, Takahiro Hino, Ryuta Miyamoto, Hitoshi Inada, Ikue Mori, Makoto Koga, Koji Miyahara, Yasumi Ohshima, Takeshi Ishihara, The Importance of cGMP Signaling in Sensory Cilia for Body Size Regulation in Caenorhabditis elegans, GENETICS, 10.1534/genetics.115.177543, 201, 4, 1497-+, 2015.12.
13. James Dillon, Christopher J. Franks, Caitriona Murray, Richard J. Edwards, Fernando Calahorro, Takeshi Ishihara, Isao Katsura, Lindy Holden-Dye, Vincent O'Connor, Metabotropic glutamate receptors
Modulators of context-dependent feeding behaviour in C. elegans, Journal of Biological Chemistry, 10.1074/jbc.M114.606608, 290, 24, 15052-15065, 2015.06, Glutamatergic neurotransmission is evolutionarily conserved across animal phyla. A major class of glutamate receptors consists of the metabotropic glutamate receptors (mGluRs). In C. elegans, three mGluR genes, mgl-1, mgl-2, and mgl-3, are organized into three subgroups, similar to their mammalian counterparts. Cellular reporters identified expression of the mgls in the nervous system of C. elegans and overlapping expression in the pharyngeal microcircuit that controls pharyngeal muscle activity and feeding behavior. The overlapping expression of mgls within this circuit allowed the investigation of receptor signaling per se and in the context of receptor interactions within a neural network that regulates feeding.Weutilized the pharmacological manipulation of neuronally regulated pumping of the pharyngeal muscle in the wild-type and mutants to investigate MGL function. This defined a net mgl-1-dependent inhibition of pharyngeal pumping that is modulated by mgl-3 excitation. Optogenetic activation of the pharyngeal glutamatergic inputs combined with electrophysiological recordings from the isolated pharyngeal preparations provided further evidence for a presynaptic mgl-1-dependent regulation of pharyngeal activity. Analysis of mgl-1, mgl-2, and mgl-3 mutant feeding behavior in the intact organism after acute food removal identified a significant role for mgl-1 in the regulation of an adaptive feeding response. Our data describe the molecular and cellular organization of mgl-1, mgl-2, and mgl-3. Pharmacological analysis identified that, in these paradigms, mgl-1 and mgl-3, but not mgl-2, can modulate the pharyngeal microcircuit. Behavioral analysis identified mgl-1 as a significant determinant of the glutamate-dependent modulation of feeding, further highlighting the significance of mGluRs in complex C. elegans behavior..
14. Terumasa Tokunaga, Osamu Hirose, Shotaro Kawaguchi, Yu Toyoshima, Takayuki Teramoto, Hisaki Ikebata, Sayuri Kuge, Takeshi Ishihara, Yuichi Iino, Ryo Yoshida, Automated detection and tracking of many cells by using 4D live-cell imaging data, BIOINFORMATICS, 10.1093/bioinformatics/btu271, 30, 12, 43-51, 2014.06.
15. Toshio Shibata, Sanae Sekihara, Takumi Fujikawa, Ryuta Miyaji, Kouki Maki, Takeshi Ishihara, Takumi Koshiba, Shun-Ichiro Kawabata, Transglutaminase-catalyzed protein-protein cross-linking suppresses the activity of the NF-κB-like transcription factor relish, Science Signaling, 10.1126/scisignal.2003970, 6, 285, 2013.07, Cross-linking of proteins by mammalian transglutaminases (TGs) plays important roles in physiological phenomena such as blood coagulation and skin formation. We show that Drosophila TG suppressed innate immune signaling in the gut. RNA interference (RNAi) directed against TG reduced the life span of flies reared under conventional nonsterile conditions but not of those raised under germ-free conditions. In conventionally reared flies, TG RNAi enhanced the expression of genes encoding antimicrobial peptides in the immune deficiency (IMD) pathway. Wild-type flies that ingested gut lysates prepared from conventionally reared TG RNAi-treated flies had shorter life spans. In conventionally reared flies, TG RNAi triggered apoptosis in the gut and induced the nuclear translocation of Relish, the NF-κB (nuclear factor κB)-like transcription factor of the IMD pathway. Wild-type flies that ingested synthetic amine donors, which inhibit the TG-catalyzed protein-protein cross-linking reaction, showed nuclear translocation of Relish and enhanced expression of genes encoding IMD-controlled antimicrobial peptide genes in the gut. We conclude that TG-catalyzed Relish cross-linking suppressed the IMD signaling pathway to enable immune tolerance against commensal microbes..
16. Akitoshi Inoue, Etsuko Sawatari, Naoki Hisamoto, Tomohiro Kitazono, Takayuki Teramoto, Manabi Fujiwara, Kunihiro Matsumoto, Takeshi Ishihara, Forgetting in C. elegans is accelerageted by neuronal communication via the TIR-1/JNK-1 pathway, Cell Reports, 10.1016/j.celrep., 3, 3, 809-819, 2013.03, 線虫の嗅覚順応をモデルとして、忘却機構の解析を行ったところ、忘却を促進するシグナルが外部環境に応じて放出されていることが明らかになった。これは能動的な忘却制御システムがあることを示している。.
17. Osamu Sasaki, Misa Kuboyama, Takeshi Ishihara, Emi Suzuki, Shun-ichiro Kawabata, TAKUMI KOSHIBA, A structural perspective of the MAVS-regulatory mechanism on the mitochondrial outer membrane using bioluminescence resonance energy transfer, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 10.1016/j.bbamcr.2013.01.010, 1833, 5, 1017-1027, 2013.02.
18. T. Uozumi, Takaaki Hirotsu, K. Yoshida, R. Yamada, A. Suzuki, G. Taniguchi, Y. Iino, Takeshi Ishihara, Temporally-regulated quick activation and inactivation of Ras is important for olfactory behaviour, SCIENTIFIC REPORTS, 10.1038/srep00500, 2, 2012.07.
19. Yoshida K, Hirotsu T, Tagawa T, Oda S, Wakabayashi T, Iino Y, Ishihara T., Odour concentration -dependent olfactory preference change in C. elegans., Nature Communications, 3, 739, 2012.03, 動物は、同じ刺激であっても強さに応答を変える場合がある。線虫においては、低濃度では誘引される物質に対して高濃度では忌避するという現象がある。このメカニズムを解析したところ、主に方向転換を使って忌避していること、低濃度の場合と高濃度の場合で感覚するニューロンが異なっていることなどが明らかになった。.
20. Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE, An expanded palette of genetically encoded Ca2+ indicators, Science, 333, 1888-91, 2011.09, 細胞内のCa2+の変化を、蛍光タンパク質Ca2+センサーを用いて調べることができる。この論文では、蛍光タンパク質Ca2+センサーに変異を導入し、これまでの数倍感度が高いセンサーを開発した。さらに、これまでなかった、赤色蛍光タンパク質Ca2+センサーを開発した。これらのセンサーは、培養細胞だけでなく個体で特定の神経細胞での活動測定にも有用であることを明らかにした。.
21. Shinkai Y, Yamamoto Y, Fujiwara M, Tabata T, Murayama T, Hirotsu T, Ikeda DD, Tsunozaki M, Iino Y, Bargmann CI, Katsura I, Ishihara T., Behavioral choice between conflicting alternatives is regulated by a receptor guanylyl cyclase, GCY-28, and a receptor tyrosine kinase, SCD-2, in AIA interneurons of Caenorhabditis elegans., Journal of Neuroscience, 31, 8, 3007 , 2011.02, 動物は、同時に受容している多くの感覚情報のなかから、生存に適した情報を選択して、それに応答して行動している。しかし、その制御を行っている神経・分子メカニズムは、明らかになっていない。本研究では、線虫C. elegansをモデルとして、2つの相反する刺激に対する応答を解析した。その結果、一対に介在ニューロンにおいて、グアニル酸シクラーゼと受容体チロシンキナーゼを介して、情報処理が行われていることを明らかにした。.
22. Fujiwara M, Teramoto T, Ishihara T, Ohshima Y, McIntire SL., 2.A novel zf-MYND protein, CHB-3, mediates guanylyl cyclase localization to sensory cilia and controls body size of Caenorhabditis elegans.
, Plos Genetics, 6, 11, e1001211., 2010.11.
23. Yamada K, Hirotsu T, Matsuki M, Butcher RA, Tomioka M, Ishihara T, Clardy J, Kunitomo H, Iino Y., Olfactory plasticity is regulated by pheromonal signaling in Caenorhabditis elegans., Science. , 329, 5999, 1647-1650, 2010.09, 動物は、集団密度などの環境によって、外界シグナルに対する応答を変化させる。線虫C. elegansをモデルとして、動物の集団密度が行動可塑性に与える影響を解析した。その結果、線虫は、集団密度の情報をフェロモンとして受容し、ペプチド性シグナルSNET-1と、そのシグナル強度を変化させるNEP-2を介して、嗅覚順応の制御を行っていることを明らかにした。.
24. Oishi A., Gengyo-Ando K., Mitani S., Mohri-Shiomi A., Kimura K.D., Ishihara T., Katsura I., FLR-2, the glycoprotein hormone alpha subunit, is involved in the neural control of intestinal functions in Caenorhabditis elegans., Genes to Cells, 14, 1141-54, 2009.07.
25. Hirotsu T., Hayashi Y., Iwata R., Kunitomo H., Kage-Nakadai E., Kubo T., Ishihara T. and Iino Y., Behavioural assay for olfactory plasticity in C. elegans., Nature Protocols, 139, 2009.07.
26. Hayashi Y., Hirotsu T., Iwata R., Kage-Nakadai E., Kunitomo H., Ishihara T., Iino Y. and Kubo T., A trophic role for Wnt-Ror kinase signaling during developmental pruning in Caenorhabditis elegans., Nature Neuroscience, 12, 981-987, 2009.06.
27. Ohta, H., Fujiwara, M., Ohshima, Y. and Ishihara, T. , ADBP-1 regulates an ADAR RNA-editing enzyme to antagonize RNAi-mediated gene silencing in C.elegans., Genetics, 180, 785-796, 2008.07.
28. Bauer Huang,SL., Saheki, Y., Vanhoven, MK., Torayama, I., Ishihara, T., Katsura, I, van der Linden, A., Sengupta, P. and Bargmann, CI., Left-right olfactory asymmetry results from antagonistic functions of voltage-activated calcium channels and the Raw repeat protein OLRN-1 in C. elegans., Neural Development, 2,24 (PMID17986337), 2007.12.
29. Eki, T., Ishihara ,T., Katsura, I. & Hanaoka, F., A Genome-wide Survey and Systematic RNAi-based Characterization of Helicase-like Genes in Caenorhabditis elegans.
, DNA Research , 14,183-199, 2007.08.
30. Kobayashi T, Gengyo-Ando K, Ishihara T, Katsura I, Mitani S, IFT-81 and IFT74 are required for intraflagellar transport in C. elegans, Genes To Cells, 12, 593-602, 2007.05.
31. Watanabe N, Ishihara T, Ohshima Y, Two mutations lead to a super small body size in the nematode C. elegans, Genes to Cells, 12, 603-612, 2007.05.
32. Torayama I, Ishihara T, Katsura I, Caenorhabditis elegans integrates the signals of butanone and food to enhance chemotaxis to butanone., Journal of Neuroscience, 27,741-50, 2007.01.
33. Yabe T., Suzuki N., Furukawa T.,Ishihara T.,Katsura I., Multidrug resistance-associated protein MRP-1 regulates dauer diapause by its export activity in Caenorhabditis elegans., Development, 132,3197-3207, 132,3197-3207(2005), 2005.01.
34. Take-Uchi M, Kobayashi Y, Kimura KD, Ishihara T, Katsura I., "FLR-4, a novel Serine/Threonine protein kinase, regulates defecation rhythm in Caenorhabditis elegans.", Mol Biol Cell, 16.1355-1365(2005), 16.1355-1365(2005), 2005.01.
35. Miyahara,K., Suzuki,N., Ishihara,T., Tsuchiya,E., Katsura,I., TBX2/TBX3 transcriptional factor homolog controls olfactory adaptation
in Caenorhabditis elegans, Journal of Neurobiology, 58, 392-402 (2004), 58, 392-402 (2004), 2004.01.
36. Ohkura,K, Suzuki,N., Ishihara,T., Katsura,I., SDF-9, a protein tyrosine pshophatse-like molecule, regulates the
L3/dauer developmental decision through hormonal signaling in C.elegans., Development, 130, 3237-3248, 2003.01.
37. Ishihara,T. Iino,Y. Mohri,A., Mori,I., Gengyo-Ando,K., Mitani,S., Katsura,I, HEN-1, a secretory protein with an LDL receptor motif, regulates
sensory integration and learning in Caenorhabditis elegans, Cell, 109, 639-49, 2002.01.
38. Shioi,G., Shoji,M., Nakamura,M., Ishihara,T., Katsura,I., Fujisawa,H., and Takagi,S, Mutations affecting nerve attachment of Caenorhabditis elegans, Genetics, 157, 1611-1622, 2001.01.
39. Asahina,M., Ishihara,T., Jindra,M., Kohara,Y., Katsura,I. and Hirose,S, The conserved nuclear receptor Ftz-F1 is required for embryogenesis,
moultingand reproduction in Caenorhabditis elegans, Genes to Cells, 5, 711-723, 2000.01.
40. Aoki,H., Sato,S., Takanami,T., Ishihara,T., Katsura,I., Takahashi,H., and Higashitani,A., Characterization of Ce-atl-1, an ATM-like gene from Caenorhabditis
elegans., Molecular and General Genetics, 264, 119-126, 2000.01.
41. Okuda,T., Haga,T., Kanai,Y., Endou,H., Ishihara,T., and Katsura,I, Identification and characterization of the high-affinity
cholinetransporter, Nature Neuroscience, 3, 120-125, 2000.01.
42. Hashimoto,H. Nishino,A. Shintani,N., Hagihara,N. and Copeland,N.G., Jenkins,N.A., Yamamoto,K., Matsuda,T., Ishihara,T., Nagata,S., and Baba,A., Genomic Organization and Chromosomal Location of the Mouse Vasoactive
Intestinal Polypeptide 1 (VPAC1) Receptor, Genomics, 58, 90-93, 1999.01.
43. Fujiwara,M., Ishihara,T., and Katsura,I, A novel WD40 protein, CHE-2, acts cell-autonomously in the formation
of C. elegans sensory cilia., Development, 126, 4839-4848, 1999.01.
44. Winnier,A.R., Meir,J.Y.-J., Ross,J.M., Tavernarakis,N., Driscoll,M.,Ishihara,T., Katsura,I., and Miller III,D.M., UNC-4/UNC-37-dependent repression of motor neuron-specific genes
controls synaptic choice in Caenorhabditis elegans., Genes and Development, 13, 2774-2786, 1999.01.
45. Take-uchi,M. Kawakami,M., Ishihara,T., Amano, K.Kondo, and I.Katsura, An ion channel of the degenerin/epithelial sodium channel superfamily
controls the defecation rhythm in Caenorhabditis elegans., Proc.Natl.Acad.Sci.USA, 95, 11775-11780, 1998.01.
46. Takanami,T., Sato, S., Ishihara,T., Katsura,I. Takahashi,H. and Higashitani,A., Characterization of a Caenorhabditis elegans recA-like Gene Ce-rdh-1
Involved in Meiotic Recombination, DNA Research, 5, 373-377, 1998.01.