||Tomonori Somamoto, Teruyuki Nakanishi, Mucosal delivery of fish vaccines: Local and systemic immunity following mucosal immunisations, Fish and Shellfish Immunology, 10.1016/j.fsi.2020.01.005, 99: 199-207, 2020.02.
||Tomonori Somamoto, Erling Olaf Koppang, Uwe Fischer, Antiviral functions of CD8(+) cytotoxic T cells in teleost fish, DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 10.1016/j.dci.2013.07.014, Vol.43, No.2, pp.197-204, 2014.04, Cytotoxic T-cells (CTLs) play a pivotal role in eliminating viruses in mammalian adaptive immune system. Many recent studies on T-cell immunity of fish have suggested that teleost CTLs are also important for antiviral immunity. Cellular functional studies using clonal ginbuan crucian carp and rainbow trout have provided in vivo and in vitro evidence that in many respects, virus-specific CTLs of fish have functions similar to those of mammalian CTLs. In addition, mRNA expression profiles of CTL-related molecules, such as CD8, TCR and MHC class I, have shown that in a wide range of fish species, CTLs are involved in antiviral adaptive immunity. These findings are a basis to formulate possible vaccination strategies to trigger effective antiviral CTL responses in teleost fish. This review describes recent advances in our understanding of antiviral CTL functions in teleost fish and discusses vaccination strategies for efficiently inducing CTL activities. (C) 2013 Elsevier Ltd. All rights reserved..
||Teruyuki Nakanishi, Hideaki Toda, Yasuhiro Shibasaki, Tomonori Somamoto, Cytotoxic T cells in teleost fish., Developmental and comparative immunology, 10.1016/j.dci.2011.03.033, Vol.35, No.12, pp.1317-23, 2011.12, The presence of antigen-specific cytotoxic T cells has been suggested in a number of in vivo and in vitro studies in fish. Acute allograft rejection with an accelerated response on second-set grafts and the presence of graft-versus-host reaction (GVHR) has been reported in teleost. Alloantigen- and virus-specific cytotoxicity has also been demonstrated in ex vivo studies in ginbuna and rainbow trout. In addition, alloantigen-specific cytotoxic T cell clones have been produced in cultures initiated with peripheral blood leukocytes (PBL) from an alloantigen-immunized channel catfish. Over the last decade several fish genomes have been sequenced and genetic information is rapidly accumulating. Thanks to these genome data bases and EST analysis, mRNA expression of T cell surface marker genes in alloantigen- or virus-specific effector cells has been reported in some fish species, e.g. TCR α or β and CD8α in ginbuna and rainbow trout, and TCR α, β or γ in channel catfish. These findings suggest the presence of CD8(+) cytotoxic T lymphocyte (CTL) in fish similar to those of higher vertebrates. Recently, monoclonal antibodies against CD8α and CD4 antigens have been produced in some fish species. Investigation on the characteristics of CTL and cell-mediated immune mechanisms is now possible at defined T cell subsets, although identification of T cell subset is limited in a few fish species at present. In this review, we describe the recent progress in this field focusing on cells involved in antigen specific cytotoxicity..
||Miki Nakao, Masakazu Tsujikura, Satoko Ichiki, Tam K. Vo, Tomonori Somamoto, The complement system in teleost fish: Progress of post-homolog-hunting researches, DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 10.1016/j.dci.2011.03.003, Vol.35, No.12, pp.1296-1308, 2011.12, Studies on the complement system of bony fish are now finishing a stage of homologue-hunting identification of the components, unveiling existence of almost all the orthologues of mammalian complement components in teleost. Genomic and transcriptomic data for several teleost species have contributed much for the homologue-hunting research progress. Only an exception is identification of orthologues of mammalian complement regulatory proteins and complement receptors. It is of particular interest that teleost complement components often exist as multiple isoforms with possible functional divergence. This review summarizes research progress of teleost complement system following the molecular identification and sequence analysis of the components. The findings of extensive expression analyses of the complement components with special emphasis of their prominent extrahepatic expression, acute-phase response to immunostimulation and various microbial infections, and ontogenic development including maternal transfer are discussed to infer teleost-specific functions of the complement system. Importance of the protein level characterization of the complement components is also emphasized, especially for understanding of the isotypic diversity of the components, a unique feature of teleost complement system. (C) 2011 Elsevier Ltd. All rights reserved..
||Cytotoxic T cell function in fish..
||Uwe Fischer, Katrin Utke, Tomonori Somamoto, Bernd Köllner, Mitsuru Ototake, Teruyuki Nakanishi, Cytotoxic activities of fish leucocytes, Fish & Shellfish Immunology, 20,209-26., 2006.02.
||Nakao M, Kato-Unoki Y, Nakahara M, Mutsuro J and Somamoto T,, Diversified components of bony fish complement system: More genes for robuster innate defense?, Experimental Medicine and Biology, in press, 2006.01.