Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Tomonori Somamoto Last modified date:2023.08.08

Associate Professor / Marine Biological Chemistry / Department of Bioscience and Biotechnology / Faculty of Agriculture

1. Sukeda M, Prakash H, Nagasawa T, Nakao M, Somamoto T., Non-specific cytotoxic cell receptor protein-1 (NCCRP-1) is involved in anti-parasite innate CD8+ T cell-mediated cytotoxicity in ginbuna crucian carp, Fish Shellfish Immunol, doi: 10.1016/j.fsi.2023.108904., 139, 108904., 2023.08.
2. Nehlah R, Yamamoto A, Nagasawa A, Somamoto T and Miki Nakao., Functional Analysis of Two Divergent C4 Isotypes in the Classical and Lectin Pathways of Complement Activation in the Common Carp, Cyprinus carpio. , Journal of Marine Science and Engineering,, 11, 707, 2023.03.
3. Prakash H, Sato M, Kojima K, Sato A, Maruyama S, Nagasawa T, Nakao M, Somamoto T, Development of a filter device for the prevention of aquatic bacterial disease using a single-chain variable fragment (scFv)-conjugated affinity silk., Scientific Reports,, 12, 9475, 2022.06.
4. Shiota K, Sukeda M, Prakasha H, Kondo M, Nakanishi T, Nagasawa T, Nakao M, Somamoto T., Local immune responses to two stages of Ichthyophthirius multifiliis in ginbuna crucian carp., Fish and Shellfish Immunology,, 118, 19-24, 2021.11.
5. Harsha Prakash, Shiori Motobe, Takahiro Nagasawa, Tomonori Somamoto, Miki Nakao , Homeostatic Functions of Tecrem, a CD46-Like Regulatory Protein of Complement Activation, on Epithelial Cells in Carp Fish. , Journal of Marine Science and Engineering,, 9, 7, 687, 2021.06.
6. Sukeda M, Shiota K, Kondo M, Nagasawa T, Nakao M, Somamoto T., Innate cell-mediated cytotoxicity of CD8+ T cells against the protozoan parasite Ichthyophthirius multifiliis in the ginbuna crucian carp, Carassius auratus langsdorfii., Developmental and Comparative Immunology,, 115, 103886., 2021.02.
7. Matsui S., Yoshikawa S., Suzuki S., Somamoto T., Yamamoto A., Nakamura O., Tsutsuia S., Expression profile of kalliklectin, a soluble-type mannose receptor, during embryogenesis and early larval development in fugu (Takifugu rubripes)., Molecular Immunology, 10.1016/j.molimm.2020.07.022., 126, 129-135, 2020.10.
8. Seisuke Tajimi, Masakazu Kondo, Teruyuki Nakanishi, Takahiro Nagasawa, Miki Nakao, Tomonori Somamoto, Generation of virus-specific CD8+ T cells by vaccination with inactivated virus in the intestine of ginbuna crucian carp., Developmental and comparative immunology, 10.1016/j.dci.2018.12.009, 93, 37-44, 2019.04.
9. Tomonori Somamoto, Shinpei Maruyama, Takahiro Nagasawa, Miki Nakao, Atsushi Sato, Hajime Hatta, Mitsuru Sato, Yukie Murakami-Yamaguchi, Kumiko Kizu-Mori, Yuki Hirakawa, Hiroshi Narita, Development of anti-atypical aeromonas salmonicida monoclonal antibodies for diagnosis of new ulcer disease in koi carp, Fish Pathology, 10.3147/jsfp.53.36, 53, 1, 36-39, 2018.03.
10. Yumie Tokunaga, Masamichi Shirouzu, Ryota Sugahara, Yasutoshi Yoshiura, Ikunari Kiryu, Mitsuru Ototake, Takahiro Nagasawa, Tomonori Somamoto, Miki Nakao, Comprehensive validation of T- and B-cell deficiency in rag1-null zebrafish: Implication for the robust innate defense mechanisms of teleosts., Scientific reports, 10.1038/s41598-017-08000-2, 7, 1, 7536-7536, 2017.08.
11. Indriyani Nur, Nevien K. Abdelkhalek, Shiori Motobe, Ryota Nakamura, Masakazu Tsujikura, Tomonori Somamoto, Miki Nakao, Functional analysis of membrane-bound complement regulatory protein on T-cell immune response in ginbuna crucian carp, Molecular Immunology, 10.1016/j.molimm.2015.11.010, 70, 1-7, 2016.02.
12. Tomonori Somamoto, Yuhei Miura, Teruyuki Nakanishi, Miki Nakao, Local and systemic adaptive immune responses toward viral infection via gills in ginbuna crucian carp., Developmental and comparative immunology, 10.1016/j.dci.2015.04.016, 52, 1, 81-7, 2015.09.
13. Hongjian Gan, Haiwen He, Atsushi Sato, Hajime Hatta, Miki Nakao, Tomonori Somamoto, Ulcer disease prophylaxis in koi carp by bath immersion with chicken egg yolk containing anti-Aeromonas salmonicida IgY, RESEARCH IN VETERINARY SCIENCE, 10.1016/j.rvsc.2015.01.016, 99, 82-86, 2015.04.
14. Masakazu Tsujikura, Takahiro Nagasawa, Satoko Ichiki, Ryota Nakamura, Tomonori Somamoto, Miki Nakao, A CD46-like Molecule Functional in Teleost Fish Represents an Ancestral Form of Membrane-Bound Regulators of Complement Activation, JOURNAL OF IMMUNOLOGY, 10.4049/jimmunol.1303179, 194, 1, 262-272, 2015.01.
15. Nagasawa T, Nakayasu C, Rieger AM, Barreda DR, Somamoto T, Nakao M, Phagocytosis by thrombocytes is a conserved innate immune mechanism in lower vertebrates. , Frontiers in Immunology, doi: 10.3389/fimmu.2014.00445, 5, 445, 2014.09.
16. Tomonori Somamoto, Masakazu Kondo, Teruyuki Nakanishi, Miki Nakao, Helper function of CD4⁺ lymphocytes in antiviral immunity in ginbuna crucian carp, Carassius auratus langsdorfii., Developmental and comparative immunology, 10.1016/j.dci.2013.12.008, 44, 1, 111-5, 2014.05.
17. Soha G. R. Abdel-Salam, Masakazu Tsujikura, Masakazu Kondo, Tomonori Somamoto, Miki Nakao, Purification and functional characterization of complement C3 and a novel zymosan-binding protein in tilapia serum, FISHERIES SCIENCE, 10.1007/s12562-014-0700-7, 80, 2, 301-310, 2014.03, Zymosan, a yeast cell wall preparation that binds activated forms of complement C3, is a useful model target to activate the complement system. In our trial to analyze C3 diversity in Nile tilapia at the protein level using zymosan, we found that a novel 240-kDa serum protein (ZBP-240) also bound to zymosan in addition to C3-derived fragments. In the present study, we aimed to characterize tilapia C3 and ZBP-240, focusing on their immune-related functions. Four distinct C3 isoforms were purified from tilapia serum and shown to possess an intrachain thioester bond. ZBP-240 was also isolated from tilapia serum and examined for its binding properties to various microbial targets. As a result, ZBP-240 showed a wide spectrum of binding to Gram-positive and Gram-negative bacteria and yeasts. Amino acid sequence analysis of CNBr fragments of ZBP-240 suggested that this is a novel protein with no homologous sequence in protein databases. It was also suggested that the binding of ZBP-240 to microbes largely depends on hydrophobic interactions in a divalent-cation-independent manner, and that there may be a divalent-cation-dependent factor that enhances the binding of ZBP-240 in tilapia serum. Interestingly, ZBP-240 showed opsonic activity for tilapia kidney phagocytes at a level comparable to that of C3, implying that ZBP-240 is a novel teleost opsonic serum protein..
18. Nevien K. Abdelkhalek, Asuka Komiya, Yoko Kato-Unoki, Tomonori Somamoto, Mild Nakao, Identification of Immune Relevant Genes Using Expressed Sequence Tags (ESTs) in Common carp (Cyprinus carpio) Gills and Intestine, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 59, 1, 109-116, 2014.02, Identification of immune related molecules that function in fish organs is important for better understanding of the host defense mechanisms in fish. Expressed sequence tags (ESTs) represent the expressed portion of a genome, so they have proven to be useful tool for gene identification and confirmation of gene predictions. In the present study, a transcriptome analysis of carp gill and intestine EST libraries have been done as an attempt to identify the immune relevant genes expressed in those organs. A total of 2148 EST clones were generated from the two libraries: 1099 clones from gill library in which 632 clones were matched with functional proteins and 1049 clones from intestine library in which 559 clones were matched with functional proteins. The results of gill EST library has more frequency of innate immune molecules containing MHC class I and MEG class II, which showed 18.3% of the immune molecules than those in intestine library which represents 10.8% only. The gill library also showed higher frequency of the cytokines and chemokine molecules and/or their receptors compared to the intestine library..
19. Indriyani Nur, Hikari Harada, Masakazu Tsujikura, Tomonori Somamoto, Miki Nakao, Molecular characterization and expression analysis of three membrane-bound complement regulatory protein isoforms in the ginbuna crucian carp Carassius auratus langsdorfii, Fish & shellfish immunology , 10.1016/j.fsi.2013.08.002, 35, 4, 1333-1337, 2013.10.
20. Tomonori Somamoto, Teruyuki Nakanishi, Miki Nakao, Identification of anti-viral cytotoxic effector cells in the ginbuna crucian carp, Carassius auratus langsdorfii., Developmental and comparative immunology, 10.1016/j.dci.2012.11.001, 39, 4, 370-7, 2013.04.
21. Satoko Ichiki, Yoko Kato-Unoki, Tomonori Somamoto, Miki Nakao, The binding spectra of carp C3 isotypes against natural targets independent of the binding specificity of their thioester., Developmental and comparative immunology, 10.1016/j.dci.2012.03.004, 38, 1, 10-6, 2012.09, The central component of complement, C3, plays a versatile role in innate immune defense of vertebrates and some invertebrates. A notable molecular characteristic of this component is an intra-chain thioester site that enables C3 to bind covalently to its target. It has been reported that the binding preference of the thioester to hydroxyl or amino groups is primarily defined by presence or absence of the catalytic histidine residue at position 1126 in human C3. In teleosts, a unique C3 (non-His type) has been found, in addition to the common His type C3. These distinct C3 isoforms may provide diversity in the target-binding attributable to the different binding specificities of their thioesters. In the present study, we examine the hypothesized correlation of the catalytic histidine with the binding spectra of two major C3 isotypes of carp towards various model and natural targets. The results reveal that non-His type C3, rather than His type C3, has a wider range of binding spectrum, despite the binding specificity of its thioester being limited to amino groups. It is therefore hypothesized that the binding spectra of C3 isotypes are not defined by the binding specificity of the thioester but is more affected by differences in microbe-associated molecular patterns that activate complement. Overall, the present data imply that non-His type C3 plays a significant role against bacterial infections in the fish defense system..
22. Sameshima S, Nakao M, Somamoto T. , Diversity of CD2 subfamily receptors in cyprinid fishes., Result Immunol, 2, 25-34, 2012.02.
23. Shinji Urabe, Tomonori Somamoto, Shiro Sameshima, Yoko Unoki-Kato, Teruyuki Nakanishi, Miki Nakao, Molecular characterization of MHC class I and beta-2 microglobulin in a clonal strain of ginbuna crucian carp, Carassius auratus langsdorfii., Fish & shellfish immunology, 10.1016/j.fsi.2011.06.004, 31, 3, 469-74, 2011.09.
24. Hideaki Toda, Yasutaka Saito, Takuhito Koike, Fumio Takizawa, Kyosuke Araki, Takeshi Yabu, Tomonori Somamoto, Hiroaki Suetake, Yuzuru Suzuki, Mitsuru Ototake, Tadaaki Moritomo, Teruyuki Nakanishi, Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish., Developmental and comparative immunology, 10.1016/j.dci.2011.01.013, 35, 6, 650-60, 2011.06, The presence of helper and cytotoxic T cells in fish has been suggested, although T cell subsets have yet to be identified at the cellular level. In order to investigate the functions of CD4 and CD8α positive T cells we attempted to produce and characterize monoclonal antibodies (mAbs) against teleost CD4 and CD8α. Here we report the successful production of mAbs against CD4 and CD8α in clonal ginbuna crucian carp Carassius auratus langsdorfii and the function of CD4 positive T cells. In this study we demonstrate the presence of teleost CD4- and CD8α-positive T cell subsets with morphology, tissue distribution and gene expression similar to those of mammalian CD4- and CD8-positive T lymphocytes. Using mAbs we found that CD4/CD8 double positive T cells are only present in the thymus, suggesting that it is the site of T cell development. We further demonstrated in vitro proliferation of CD4 positive T cells by allogeneic combination of mixed leukocyte culture and antigen-specific proliferation of CD4 positive T cells after in vitro sensitization with OVA. In our previous study we showed that CD8α-positive lymphocytes are the primary cell type showing specific cytotoxicity against allogeneic targets. Collectively, these findings suggest that CD4 and CD8α positive T cells in ginbuna are equivalent to helper and cytotoxic T lymphocytes (CTL) in mammals, respectively. This is the first report to show the characteristics and functions of CD4 positive T cells in fish and these findings shed light into the evolutionary origins and primordial functions of helper T cells..
25. Nevien K. Abdelkhalek, Asuka Komiya, Yoko Kato-Unoki, Tomonori Somamoto, Miki Nakao, Molecular evidence for the existence of two distinct IL-8 lineages of teleost CXC-chemokines, Fish & shellfish immunology , 10.1016/j.fsi.2009.08.004, 27, 6, 763-767, 2009.12.
26. Vo Kha Tam, Masakazu Tsujikura, Tomonori Somamoto, Miki Nakao, Expression Responses of the Complement Components in Zebrafish Organs after Stimulation with Poly I:C, Mimicry of Viral Infection, JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 54, 2, 389-395, 2009.10, Defensive roles of the complement system in fish are largely unknown, although expressions of several complement components have been examined in some teleost species upon bacterial and parasitic infections. To infer possible anti-virus defensive roles of the complement system in teleost, zebrafish were simulated by intraperitoneal injection of poly 1: C, a double strand RNA analogue, to mimic viral infection, and expression of 24 complement components and their subunits in the gill, brain, kidney, gut, hepatopancreas, gonad, heart, spleen, and skin were analyzed by reverse-transcription PCR. In normal (unstimulated) fish, the hepatopancreas showed highest expression of most genes tested, and considerable extra hepatic expression was also observed for many complement components. Upon stimulation, the hepatopancreas showed decreased expression of CD11/CD18, B/C2-A3, MASP3, and properdin, while marked up-regulation of several complement component genes was observed in the gill, suggesting an important but unknown role of the gill in host, defense against viral infection..
27. Vo Kha Tam, Masakazu Tsujikura, Tomonori Somamoto, Miki Nakao, Identification of cDNA Sequences Encoding the Complement Components of Zebrafish (Danio rerio), JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY, 54, 2, 373-387, 2009.10, Zebrafish can be utilized for immunological research as a fish model that allows gene targeting, but lacks enough molecular and sequence information on the complement system, a humoral innate immune factor. The present study aimed at identification of all the major complement components at the molecular level with phylogenetic evidence. Complementary DNA sequences encoding C1q, C1r/s, C2 (B/C2-B), C3, C4, C5, C6, C7, C8, C9, factor B (B/C2-A), the mannose-binding lectin (MBL), two MBL-associated serine proteases, properdin, and factor I were identified by cloning from the hepatopancreas and by database mining. As reported for carp and trout, the presence of two distinct isotypes were confirmed for C3, C4, and C7 also in zebrafish, indicating the ancient evolutionary origin of their diversifications in the vertebrate lineage..
28. Tomonori Somamoto, Nobuaki Okamoto, Teruyuki Nakanishi, Mitsuru Ototake, Miki Nakao, In vitro generation of viral-antigen dependent cytotoxic T-cells from ginbuna crucian carp, Carassius auratus langsdorfii., Virology, 10.1016/j.virol.2009.04.008, 389, 1-2, 26-33, 2009.06.
29. Seiko Nonaka, Tomonori Somamoto, Yoko Kato-Unoki, Mitsuru Ototake, Teruyuki Nakanishi, Miki Nakao, Molecular cloning of CD4 from ginbuna crucian carp Carassius auratus langsdorfii, FISHERIES SCIENCE, 10.1111/j.1444-2906.2008.01530.x, 74, 2, 341-346, 74: 341-346., 2008.04, The clonal triploid ginbuna crucian carp Carassius auratus langsdorfii, a naturally occurring gynogenetic fish, is a useful model for studying T-cell-mediated immunity. CD4, a T-cell receptor (TCR) coreceptor, is a membrane-bound glycoprotein found on helper T-cells, and assists in the binding of major histocompatibility complexes. In the present study, full-length cDNAs encoding the CD4 molecule from the S3n strain of ginbuna crucian carp were cloned and characterized. 5'-rapid amplification of cDNA ends (RACE) and 3'-RACE yielded two distinct cDNA clones of CD4 homolog from the ginbuna, and these sequences share 95% identity at the amino acid level. These ginbuna CD4 molecules consisted of a signal peptide, immunoglobulin superfamily (IgSf) like domains, a transmembrane domain, and a cytoplasmic domain similar to other known CD4. A tyrosine protein kinase p56(lck) binding motif is conserved in the cytoplasmic tail of ginbuna CD4. Phylogenetic tree analysis indicated that ginbuna CD4 sequences are closely related to CD4L-1 from other fish species. Expression of ginbuna CD4 mRNA was detected in the gill, thymus, head kidney, trunk kidney and peripheral blood leukocytes, indicating that its expression pattern is similar to that of ginbuna TCR beta mRNA. The results suggest that ginbuna CD4 sequences are useful as molecular probes for helper T-cells..
30. Suetake H, Araki K, Akatsu K, Somamoto T, Dijkstra J. M, Yoshiura Y, Kikuchi K, Suzuki Y, Genomic organization and expression of CD8α and CD8β genes in fugu Takifugu rubripes, Fish Shellfish Immunol, 10.1016/j.fsi.2007.05.005, 23, 5, 1107-18, 23(5):1107-18, 2007.11.
31. Miki Nakao, Takayuki Kajiya, Yuho Sato, Tomonori Somamoto, Yoko Unoki-Kato, Misao Matsushita, Munehiro Nakata, Teizo Fujita and Tomoki Yano. , Lectin pathway of bony fish complement:Identification of two homologues of the mannose-binding lectin associated with MASP2 in the common carp (Cyprinus carpio) , Journal of Immunology , 177:5471-5479., 2006.10.
32. Tomonori Somamoto, Yasutoshi Yoshiura, Atsushi Sato, Miki Nakao, Teruyuki Nakanishi, Nobuaki Okamoto, Mitsuru Ototake, Expression profiles of TCRβ and CD8α mRNA correlate with virus-specific cell-mediated cytotoxic activity in ginbuna crucian carp, Virology, 348: 370-377, 2006.05.
33. J.M. Dijkstra, T. Somamoto, L. Moore, I. Hordvik, M. Ototake, and U. Fischer., Identification and characterization of a second CD4-like gene in teleost fish., Molecular Immunology, 43: 410-419., 2006.02.
34. Tomonori Somamoto, Yasutoshi Yoshiura, Teruyuki Nakanishi, Mitsuru Ototake., Molecular cloning and characterization of two types of CD8α from ginbuna crucian carp, Carassius auratus langsdorfii., Developmental and Comparative Immunology, 29,693-702, 2005.07.
35. L J Moore, T Somamoto, K K Lie, J M Dijkstra, I Hordvik, Characterisation of salmon and trout CD8alpha and CD8beta., Molecular immunology, 42, 10, 1225-34, 2005.06, The genes and corresponding cDNAs of both alpha and beta chains of the Atlantic salmon (Salmo salar) CD8 molecule have been sequenced and characterized. In addition, the cDNAs for alpha and beta chains of brown trout (Salmo trutta) and for the beta chain in rainbow trout (Oncorhynchus mykiss) have been sequenced. The cDNAs code for signal sequences which are preceded by short 5' UTRs. These are followed by typical immunoglobulin superfamily variable sequences all of which contain two conserved cysteines for the intra-chain disulphide bond. The hinge regions display conserved cysteines for dimerisation and several O-glycosylation motifs for each predicted protein. The domain sharing the highest sequence identity with mammals is the single pass transmembrane domain for all sequences. In salmon, each domain is predominantly coded for by a single exon except the cytoplasmic/3' UTR domains, which are coded for by 3 and 2 exons for the alpha and beta genes, respectively. In the alpha gene, the second cytoplasmic exon may be spliced out to form an alternative shorter transcript which if expressed would exhibit a truncated cytoplasmic tail. A splice variant found for the salmon beta gene introduces a stop codon after only 40 amino acids. Overall amino acid identities between salmonid sequences were higher than 90%, whereas they shared only 15-20% identity with species such as, chicken and human. Analysis of the expression patterns of the two salmon genes using quantitative RT-PCR shows a very high expression in the thymus. This is mirrored by the expression of the TCRalpha gene, which is known to be co-expressed with CD8 on mammalian T cells. This is the first report of a sequence for CD8beta in a teleost and together with the CD8alpha sequence, it encodes the ortholog of the CD8 co-receptor molecule on mammalian T cells..
36. Atsushi Sato, Tomonori Somamoto, Hiroyuki Yokooka, Nobuaki Okamoto., Systemic priming of alloreactive cytotoxic cells in carp, following anal administration of allogeneic cell antigens., Fish & Shellfish Immunology, 19,43-52., 2005.01.
37. L.J. Moore, T. Somamoto, K.K. Lie, J.M. Dijkstra and I. Hordvik., Characterisation of salmon and trout CD8α and CD8β., Molecular Immunology, 42: 1225-1234., 2005.01.
38. Chihaya Nakayasu, Tomonori Somamoto, Satoshi Hasegawa, Tomoyasu Yoshitomi, and Nobuaki Okamoto., Differential spontaneous killing of human and murine tumor cell lines by leucocyte subpopulations from carp peripheral blood leucocytes., Fish & Shellfish Immunology, 19,115-126., 2005.01.
39. Tomonori Somamoto, Atsushi Sato, Teruyuki Nakanishi, Mitsuru Ototake, and Nobuaki Okamoto., Specific cytotoxic activity generated by mixed leucocyte culture in ginbuna crucian carp., Fish & Shellfish Immunology, 17,187-191, 2004.08.
40. J. Puangkae, V. Kiron, T. Somamoto, N. Okamoto, S. Sato, T. Takeuchi, T. Watanabe., Non-specific immune response of rainbow trout (Oncorhynchus mykiss Walbaum) in relation to different status of vitamin E and highly unsaturated fatty acids., Fish & Shellfish Immunology, 16,25-39., 2004.01.
41. Tomonori Somamoto, Teruyuki Nakanishi, and Nobuaki Okamoto., Role of specific cell-mediated cytotoxicity in protecting fish from viral infections., Virology, 297,120-127, 2002.03.
42. Tomonori Somamoto, Teruyuki Nakanishi, and Nobuaki Okamoto., Specific cytotoxic cells of ginbuna crucian carp are responsible for in vivo clearance of carp haematopoietic necrosis virus (CHNV)., Fisheries Science, 68, suppl.II,1147-1150, 2002.01.
43. Tomonori Somamoto, Teruyuki Nakanishi, and Nobuaki Okamoto., Specific cell-mediated cytotoxicity against a virus-infected syngeneic cell line in isogeneic ginbuna crucian carp., Developmental and Comparative Immunology, 24,633-640, 2000.01.
44. Satoshi Hasegawa, Tomonori Somamoto, Maria Raquel Moura Coimbra, Nobuaki Okamoto., In vivo priming of TNP-reactive effector cells in carp., Fish Pathology, 34,107-111, 1999.01.
45. Satoshi Hasegawa, Tomonori Somamoto, Chihaya Nakayasu, Teruyuki Nakanishi, Nobuaki Okamoto., A cell line (CFK) from fin of isogeneic ginbuna crucian carp., Fish Pathology, 32, 127-128, 1997.01.