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Prosthodontics as biological science-mesenchymal stem cell perspective-. |
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T Yamaza, Atsuta, I, Y Tsuji, T Goto, T Tanaka, Phosphatidylinositol-3 kinase plays a role in vesicle transport in the secretory pathway of cathepsin K and cystatin C in osteoclasts, BONE, Vol.32, No.5, p.S148, 2003.05. |
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Atsuta, I, T Yamaza, M Yoshinari, T Goto, MA Kido, T Kagiya, S Mino, M Shimono, T Tanaka, Ultrastructural localization of laminin-5 (gamma 2 chain) in the rat peri-implant oral mucosa around a titanium-dental implant by immuno-electron microscopy, BIOMATERIALS, 10.1016/j.biomaterials.2005.03.046, Vol.26, No.32, pp.6280-6287, 2005.11, Laminin-5 (Ln-5) is an important molecule associated with epithelial cell adhesion and migration. In the gingiva around the tooth. Ln-5 localizes within basement membranes between the junctional epithelium (JE), and the tooth or connective tissue. Recently, we reported that in the oral mucosa around a dental implant, Ln-5 is expressed within the basement membranes at the implant-peri-implant epithelium (PIE) interface, and at the PIE-connective tissue interface. However, the ultrastructural localization of Ln-5 within or along the PIE has not yet been reported. Therefore, peri-implant oral mucosa was treated with anti-Ln-5 ((7)2 chain) antibody and examined using immuno-electron microscopy. Ln-5 was localized in the cells of the innermost-third layer and basal layer of the PIE. A 100-nm-wide Ln-5-positive internal basal lamina (basement membrane) and hemidesmosomes as adhesion structures were formed at the apical portion of the implant-PIE interface. However, at the upper-middle portion of the interface, these adhesion structures were not observed. Furthermore. at the PIE connective tissue interface. the Ln-5-positive external basal lamina (basement membrane) and hemidesmosomes were partially deficient. Judging from these findings. we concluded that Ln-5 contributes to the attachment of the PIE to the titanium surface, and that PIE attached to titanium at the apical portion of the dental implant-PIE interfaced (c) 2005 Elsevier Ltd. All rights reserved.. |
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H Kajiwara, T Yamaza, M Yoshinari, T Goto, S Iyama, Atsuta, I, MA Kido, T Tanaka, The bisphosphonate pamidronate on the surface of titanium stimulates bone formation around tibial implants in rats, BIOMATERIALS, 10.1016/j.biomaterials.2004.02.072, Vol.26, No.6, pp.581-587, 2005.02, Many materials with differing surfaces have been developed for clinical implant therapy in dentistry and orthopedics. We analyzed the quantity of new bone formed in vivo around caleium-immobilized titanium implants with surfaces modified using pamidronate (PAM), a nitrogen-containing bisphosphonate (N-BP), implants of pure titanium, and titanium implants immobilized with calcium ions. New bone formation was visualized using fluorescent labeling (calcein blue and alizarin complexone) with intravenous injection at 1 and 3 weeks after implantation. After 4 weeks, undecalcified sections were prepared, and new bone formation around the implants was examined by morphometry using confocal laser scanning microscopy images. After 1 week, more new bone formed around the PAM-immobilized implant than around the calcium-immobilized and pure titanium implants. This was also seen with the new bone formation after 3 weeks. After 4 weeks, significantly more new bones were formed around the BP-immobilized implant than around the calcium ion-implanted and pure titanium implants. The new N-BP-modified titanium surface stimulates new bone formation around the implant, which might contribute to the success of implant therapy. (C) 2004 Elsevier Ltd. All rights reserved.. |
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Atsuta, I, T Yamaza, M Yoshinari, S Mino, T Goto, MA Kido, Y Terada, T Tanaka, Changes in the distribution of laminin-5 during peri-implant epithelium formation after immediate titanium implantation in rats, BIOMATERIALS, 10.1016/j.biomaterials.2004.05.033, Vol.26, No.14, pp.1751-1760, 2005.05, Laminin-5 (Ln-5), a component of the basement membrane (BM), regulates epithetial cell migration and adhesion. This study used anti-Ln-5 (gamma2chain) antibody to investigate the distribution of Ln-5 during the formation of peri-implant epithelium (PIE) in rats, and compared it to the distribution of Ln-5 during oral mucosa formation after tooth extraction. One day after extraction. the junctional epithelium (JE) had disappeared. After 3 days, new epithelium formed from the oral sulcular epithelium (OSE) and extended horizontally over the wound with Ln-5-positive cells at the leading edge. After 5 days, the epithelium exiending from the OSE on each side of the wound joined and formed additional new epithelium. The new epithelium expressed Ln-5 in the BM - After 1-2 weeks, the oral epithelium (OE) extending from the sides of the wound joined in the center. Thereafter. OSE and new epithelium disappeared, and only OE remained covering the wound. Three days after implantation (titanium), no JE remained. New epithehium formed from the keratinized OSE extending apically with Ln-5-positive cells. After 1-2 weeks. the new epithelium became the PIE and spread further apically facing the implant surface. Ln-5 was expressed at the PIE-connective tissue interface. but no( at the implant-PIE interface. Finally, after 4 weeks, Ln-5 was expressed at the implant-PIE interface. and the PIE was non-keratinized epithelium. These findings suggest that Ln-5 induces cell migration clurinq PIE formation. and that PIE originates from OSE. Furthermore, they support the hypothesis that Ln-5 contributes to the attachment of PIE 10 titanium. regardless of the delay in the synthesis and deposition of Ln-5 at the titanium-PIE interface. (C) 2004 Elsevier Ltd. All rights reserved.. |
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Topical Application of Fluvastatin Affects the Bone Healing around Implants. |
