| 1. |
Development of Differentiated Smooth Muscle Cell Culture with chick embryo gizzard. |
| 2. |
Wakana Izumi, Yuko Takuma, Wataru Mizunoya, Ryuichi Tatsumi and Mako Nakamura, Improving meat quality by regulating myogenic and adipogenic cell fate -A possible role of Paired box 7 (Pax7) during adipogenic differentiation-, nternational Symposium on Agricultural, Food, Environmental and Life Sciences in Asia (AFELiSA), 2019 (The 16th International Joint Symposium between Korea and Japan), 2019.11. |
| 3. |
Kosuke Tokunaga, Shota Akimoto, Wataru Mizunoya, Ryuichi Tatsumi and Mako Nakamura, The serum-free media allows chick gizzard smooth muscle cells in differentiated state., nternational Symposium on Agricultural, Food, Environmental and Life Sciences in Asia (AFELiSA), 2019 (The 16th International Joint Symposium between Korea and Japan), 2019.11. |
| 4. |
Hirochika Kido, Nana Imatomi, Shoko Okuda, Yoshitaka Manabe, Yuji Matsuyosi, Alaa Magdi Hassanein Elgaabari, Takashi Nakashima, Shoko Sawano, Wataru Mizunoya, Mako Nakamura, and Ryuichi Tatsumi, , HGF nitration abolishes myogenic stem satellite cell activation., nternational Symposium on Agricultural, Food, Environmental and Life Sciences in Asia (AFELiSA), 2019 (The 16th International Joint Symposium between Korea and Japan), 2019.11. |
| 5. |
Mako Nakamura, Wakana Izumi, Wataru Mizunoya, and Ryuichi Tatsumi, Paired box 7 inhibits adipogenic differentiation -A possible role of Pax7 in non-myogenic cells-, Avian Model Systems 10, 2018.06, Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in non-myogenic lineage cells. We performed experiments using stably expressed Pax7 in 3T3-L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. These cells showed decreased expression of PDGFR, PPAR and Fabp4 and inhibited forming lipid droplets. However, they could not differentiate into myogenic cells. We think the Pax7 functions to pause cell differentiation and needs co-factors to induce into a specific cell type. To see the function of Pax7 in a cell type where possible myogenic co-factors may be available, we decided to use smooth muscle cells (SMCs). The SMCs culture model with E15 chick gizzard has been developed. The cells are cultured in the serum free medium supplemented with BSA and IGF-1. The cells are currently analyzed for the marker gene expression to find out if they keep the differentiation state.. |
| 6. |
Shoko Sawano, Yusuke Komiya, Riho Ichitsubo, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Wataru Mizunoya, Muscle fiber type visualization using four anti-myosin heavy chain antibodies, The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress, 2016.08. |
| 7. |
Wataru Mizunoya, Daisuke Imoto, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Yukireitake mushroom(P. eryngii var. tuoliensis CJ.Mou)has both agonistic and antagonistic activities against peroxisome proliferator-activated receptor(PPAR)delta, The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress, 2016.08. |
| 8. |
Mulan Qahar, Wataru Mizunoya, Ryuichi Tatsumi, Mako Nakamura, Role for semaphorin 3A in regulation of myogenic stem cells proliferation, The 17th Asian-Australasian Association of Animal Production Societies Animal Science Congress, 2016.08. |
| 9. |
Mulan Qahar, Wataru Mizunoya, Ryuichi Tatsumi, Seong-Kyu Choe, Mako Nakamura, Semaphorin 3A promotes activation of Pax7, Myf5, and MyoD through inhibition of emerin expression, Molecular Mechanisms Modulating Skeletal Muscle Development and Homeostasis in Health and Disease, 2016.06. |
| 10. |
Shoko Sawano, Yuri Tajima, Johan Rung, Mako Nakamura, Ryuichi Tatsumi, Wataru Mizunoya, Yoshihide Ikeuchi, Transcriptomic and metabolomic analyses depicted distinct characteristics between slow-twitch and fast-twitch muscles in mice, ACN2015(12th Asian Congress of Nutrition), 2015.05. |
| 11. |
Yusuke Komiya, Tsuyoshi Goto, Nobuyuki Takahashi, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Teruo Kawada, Wataru Mizunoya, Eicosapentaenoic acid in fish oil is the key fatty acid to promote oxidative metabolism in rat skeletal muscle, ACN2015(12th Asian Congress of Nutrition), 2015.05. |
| 12. |
Wataru Mizunoya, Toshiya Nakamura, Daisuke Imoto, Yusuke Komiya, Fuminori Kawabata, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Effect of Yamabushitake mushroom (Hericium erinaceus) intake on functional and physicochemical properties of skeletal muscle in mice, ACN2015(12th Asian Congress of Nutrition), 2015.05. |
| 13. |
Mulan, Mako Nakamura, Takahiro Suzuki, Wataru Mizunoya, Koichi Ojima, Ryuichi Tatsumi, Yoshihide Ikeuchi, Functional interaction between Sema3A and Pax7 in proliferation and differentiation of myoblasts, International Symposium on Agriculture, Forestry, Environment and Life Sciences in Asia, 2014 (AFELiSA'14), 2014.10. |
| 14. |
Ryuichi Tatsumi, Shohei Sakaguchi, Junichi Shono, Takahiro Suzuki, Shoko Sawano, Judy E. Anderson, Mai-Khoi Q. Do, Hideaki Ohtsubo, Wataru Mizunoya, Mako Nakamura, Mitsuhiro Furuse, Yoshihide Ikeuchi, M2 macrophages may implicate in regenerative moto-neuritogenesis, by promoting myoblast migration and Sema3A expression, 2014 FASEB Science Research Conference on “Skeletal Muscle Satellite and Stem Cells, 2014.07. |
| 15. |
Takahiro Suzuki, Yuki Ohya, Koichi Ojima, Wataru Mizunoya, Shoko Sawano, Hideaki Ohtsubo, Shinichiro Nishimatsu, Judy E. Anderson, Mai-Khoi Q. Do, Mako Nakamura, Mitsuhiro Furuse, Yoshihide Ikeuchi, Tsutomu Nohno, Ryuichi Tatsumi, Sema3A secreted from satellite cells promotes slow-twitch fiber generation, 2014 FASEB Science Research Conference on “Skeletal Muscle Satellite and Stem Cells, 2014.07. |
| 16. |
Hideaki Ohtsubo, Yusuke Sato, Takahiro Suzuki, Wataru Mizunoya, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, APOBEC2 deficiency up-regulates myoblast differentiation, 2014 FASEB Science Research Conference on “Skeletal Muscle Satellite and Stem Cells, 2014.07. |
| 17. |
中村 真子, Yuko Nakajo, Suzuki Takahiro, Saori Nishioka, Yui Ueno, 水野谷 航, 辰巳 隆一, Yoshihide Ikeuchi, Regulation mechanism of muscle cells differentiation by a paired box protein, pax7 and Semaphorin 3A, 第35回日本分子生物学会, 2012.12. |
