九州大学 研究者情報
論文一覧
中村 真子(なかむら まこ) データ更新日:2020.07.08

准教授 /  農学研究院 附属国際農業教育・研究推進センター 国際交流推進ユニット


原著論文
1. Hideaki Ohtsubo, Yusuke Sato, Takahiro Suzuki, Wataru Mizunoya, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Data supporting possible implication of APOBEC2 in self-renewal functions of myogenic stem satellite cells
Toward understanding the negative regulation of myoblast differentiation, Data in Brief, 10.1016/j.dib.2017.03.051, 12, 269-273, 2017.06, [URL], This paper provides in vitro phenotypical data to show that APOBEC2, a member of apoB mRNA editing enzyme, catalytic polypeptide-like family, may implicate in self-renewal functions of myogenic stem satellite cells, namely in the re-establishment of quiescent status after activation and proliferation of myoblasts in single-myofiber culture..
2. Yusuke Sato, Hideaki Ohtsubo, Naohiro Nihei, Takane Kaneko, Yoriko Sato, Shin Ichi Adachi, Shinji Kondo, Mako Nakamura, Wataru Mizunoya, Hiroshi Iida, Ryuichi Tatsumi, Cristina Rada, Fumiaki Yoshizawa, Apobec2 deficiency causes mitochondrial defects and mitophagy in skeletal muscle, FASEB Journal, 10.1096/fj.201700493R, 32, 3, 1428-1439, 2018.03, [URL], Apobec2 is a member of the activation-induced deaminase/apolipoprotein B mRNA editing enzyme catalytic polypeptide cytidine deaminase family expressed in differentiated skeletal and cardiac muscle. We previously reported that Apobec2 deficiency in mice leads to a shift in muscle fiber type, myopathy, and diminished muscle mass. However, the mechanisms ofmyopathy caused by Apobec2 deficiency and its physiologic functions are unclear. Here we show that, although Apobec2 localizes to the sarcomeric Z-lines in mouse tissue and cultured myotubes, the sarcomeric structure is not affected in Apobec2-deficient muscle. In contrast, electron microscopy reveals enlarged mitochondria and mitochondria engulfed by autophagic vacuoles, suggesting that Apobec2 deficiency causes mitochondrial defects leading to increased mitophagy in skeletal muscle. Indeed, Apobec2 deficiency results in increased reactive oxygen species generation and depolarized mitochondria, leading to mitophagy as a defensive response. Furthermore, the exercise capacity of Apobec2-/- mice is impaired, implying Apobec2 deficiency results in ongoing muscle dysfunction. The presence of rimmed vacuoles in myofibers from 10-mo-old mice suggests that the chronic muscle damage impairs normal autophagy. We conclude that Apobec2 deficiency causes mitochondrial defects that increase muscle mitophagy, leading to myopathy and atrophy. Our findings demonstrate that Apobec2 is required for mitochondrial homeostasis to maintain normal skeletal muscle function..
3. Hideaki Ohtsubo, Yusuke Sato, Yuji Matsuyoshi, Takahiro Suzuki, Wataru Mizunoya, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Fluorescence microscopy data on expression of Paired Box Transcription Factor 7 in skeletal muscle of APOBEC2 knockout mice, Data in Brief, 10.1016/j.dib.2018.02.063, 17, 1348-1351, 2018.04, [URL], The data presented in this article are related to the research articles entitled “APOBEC2 negatively regulates myoblast differentiation in muscle regeneration” and “Data supporting possible implication of APOBEC2 in self-renewal functions of myogenic stem satellite cells: toward understanding the negative regulation of myoblast differentiation” (Ohtsubo et al., 2017a, 2017b) [1,2]. This article provides in vivo phenotypical data to show that Paired Box Transcription Factor 7 (Pax7)-positive cell number (per myofiber) is significantly lower in APOBEC2 (a member of apoB mRNA editing enzyme, catalytic polypeptide-like family)-knockout muscle than the control wild-type tissue at the same age of 8-wk-old in mice. The emerging results support an essential role for APOBEC2 in the self-renewal functions of myogenic stem satellite cells, namely the re-establishment of quiescent status after activation and proliferation of myoblasts..
4. Wakana Izumi, Yuko Takuma, Ryo Ebihara, Wataru Mizunoya, Ryuichi Tatsumi, Mako Nakamura, Paired box 7 inhibits differentiation in 3T3-L1 preadipocytes, Animal Science Journal, 10.1111/asj.13050, 89, 8, 1214-1219, 2018.08, [URL], Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in nonmyogenic lineage cells. In this study, we performed experiments using stably expressed Pax7 in 3T3-L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. We found that Pax7 represses adipogenic markers and prevents differentiation. These cells showed decreased expression of PDGFRα, PPARγ and Fabp4 and inhibited forming lipid droplets..
5. Yuji Matsuyoshi, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, Wataru Mizunoya, Isolation and purification of satellite cells from young rats by percoll density gradient centrifugation, Methods in Molecular Biology, 10.1007/978-1-4939-8897-6_6, 81-93, 2019.01, [URL], Satellite cells (SCs) are myogenic stem cells that play an important role in skeletal muscle regeneration and hypertrophy. Primary cultures of SCs are useful to analyze cell functions; however, it is difficult to obtain highly pure SCs from young rats with the conventional procedures. The purpose of this study is to establish a purification method for SC isolation from young rats and quantitatively evaluate the purification procedure employing Percoll, a common research tool to purify cells. We elucidated the purity of SCs collected by Percoll density gradient centrifugation using real-time RT-qPCR and immunocytochemistry for desmin. Percoll treatment increased the purity of SCs isolated from young rats to nearly 90%, which was comparable to that achieved with the conventional method using middle-aged rats..
6. Daisuke Mashima, Yoshiaki Oka, Takafumi Gotoh, Shozo Tomonaga, Shoko Sawano, Mako Nakamura, Ryuichi Tatsumi, Wataru Mizunoya, Correlation between skeletal muscle fiber type and free amino acid levels in Japanese Black steers, Animal Science Journal, 10.1111/asj.13185, 90, 4, 604-609, 2019.04, [URL], Free amino acids are important components of tastants and flavor precursors in meat. To clarify the correlation between muscle fiber type and free amino acids, we determined the concentrations of various free amino acids and dipeptides in samples of different muscle tissues (n = 21), collected from 26-month-old Japanese Black steers (n = 3) at 2 days postmortem. The proportions of the myosin heavy chain (MyHC), slow (MyHC1) and fast (MyHC2) isoforms were determined by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). The contents of free amino acids and dipeptides were measured by high performance liquid chromatography (HPLC). The MyHC isoform composition varied among the tissue samples. The MyHC1 proportion ranged from 6.9% ± 3.9% to 83.3% ± 16.7%. We confirmed that there was a strong positive correlation between MyHC1 composition and total free amino acid concentrations, including those for two dipeptides. Among the 31 measured free amino acids and dipeptides, 11 showed significant positive correlations and five showed significant negative correlations with MyHC1 composition. These results suggest that a high MyHC1 content induces high free amino acid contents in bovine muscles possibly because of greater oxidative metabolism. This high level of free amino acids could contribute to the intense flavor of meat that is rich in slow-twitch fibers..
7. Yusuke Komiya, Toshiya Nakamura, Momoko Ishii, Kuniyoshi Shimizu, Eri Hiraki, Fuminori Kawabata, Mako Nakamura, Ryuichi Tatsumi, Yoshihide Ikeuchi, Wataru Mizunoya, Increase in muscle endurance in mice by dietary Yamabushitake mushroom (Hericium erinaceus) possibly via activation of PPARδ, Animal Science Journal, 10.1111/asj.13199, 90, 6, 781-789, 2019.06, [URL], Skeletal muscle fiber is largely classified into two types: type 1 (slow-twitch) and type 2 (fast-twitch) fibers. Meat quality and composition of fiber types are thought to be closely related. Previous research showed that overexpression of constitutively active peroxisome proliferator-activated receptor (PPAR)δ, a nuclear receptor present in skeletal muscle, increased type 1 fibers in mice. In this study, we found that hexane extracts of Yamabushitake mushroom (Hericium erinaceus) showed PPARδ agonistic activity in vitro. Eight-week-old C57BL/6J mice were fed a diet supplemented with 5% (w/w) freeze-dried Yamabushitake mushroom for 24 hr. After the treatment period, the extensor digitorum longus (EDL) muscles were excised. The Yamabushitake-supplemented diet up-regulated the PPARδ target genes Pdk4 and Ucp3 in mouse skeletal muscles in vivo. Furthermore, feeding the Yamabushitake-supplemented diet to mice for 8 weeks resulted in a significant increase in muscle endurance. These results indicate that Yamabushitake mushroom contains PPARδ agonistic ligands and that dietary intake of Yamabushitake mushroom could activate PPARδ in skeletal muscle of mice. Unexpectedly, we observed no significant alterations in composition of muscle fiber types between the mice fed control and Yamabushitake-supplemented diets..
8. Tatsumi R., Suzuki T., Do MQ, Ohya Y., Anderson JE, Shibata A., Kawaguchi M, Ohya S, Mizunoya W., Sawano S., Komiya Y., Ichitsubo R., Ojima K., Nishimatsu SI., Nohno T, Ohsawa Y, Sunada Y, Nakamura M., Furuse M., Slow-Myofiber Commitment by Semaphorin 3A Secreted from Myogenic Stem Cells, STEM CELLS, 10.1002/stem.2639, 35, 7, 1815-1834, 2017.07.
9. Ohtsubo H., Sato Y., Suzuki T., Mizunoya W., Nakamura M., Tatsumi R., Ikeuchi Y, Data supporting possible implication of APOBEC2 in self-renewal functions of myogenic stem satellite cells: Toward understanding the negative regulation of myoblast differentiation., Data in Brief , 10.1016, 12, 269-273, 2017.04.
10. Komiya Y., Sawano S., Mashima D., Ichitsubo R., Nakamura M., Tatsumi R., Ikeuchi Y., Mizunoya W., Mouse soleus (slow) muscle shows greater intramyocellular lipid droplets accumulation than EDL (fast) muscle: Fiber type-specific analysis, Journal of Muscle Research and Cell Motility, 10.1007/s10974-017-9468-6, 2017.03.
11. Ohtsubo H., Sato Y., Suzuki T., Mizunoya W., Nakamura M., Tatsumi R., Ikeuchi Y., APOBEC2 negatively regulates myoblast differentiation in muscle regeneration, International Journal of Biochemistry and Cell Biology, 10.1016/j.biocel.2017.02.005, 85, 91-101, 2017.03.
12. Sawano S., Komiya Y., Ichitsubo R., Ohkawa Y., Nakamura M., Tatsumi R., keuchi Y, Mizunoya W., A One-Step Immunostaining Method to Visualize Rodent Muscle Fiber Type within a Single Specimen, PLoS ONE, 10.1371/journal.pone.0166080, 11, 11, 2016.11.
13. Qahar M., Takuma Y., Mizunoya W., Tatsumi R., Ikeuchi Y., Nakamura M., Semaphorin 3A promotes activation of Pax7, Myf5, and MyoD through inhibition of emerin expression in activated satellite cells, FEBS openbio, 10.1002/2211-5463.12050, 2016.03.
14. Do MK., Shimizu N., Suzuki T., Ohtsubo H., Mizunoya W., Nakamura M., Sawano S., Furuse, M., Ikeuchi Y., Anderson JE., Ryuichi Tatsumi, Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts, Physiological Reports, 10.14814/phy2.12553, 3, 9, 2015.09.
15. Komiya Y., Anderson ME., Akahoshi M., Nakamura M., Tatsumi R., Ikeuchi Y., Mizunoya W., Data in support for protocol for rat single muscle-fiber isolation and culture, Data in Brief, 10.1016/j.dib.2015.04.016, 2015.05.
16. Komiya Y., Anderson JE., Akahoshi M., Nakamura M., Tatsumi R., Ikeuchi Y., Mizunoya W., Protocol for rat single muscle-fiber isolation and culture, Analytical Biochemistry, 10.1016/j.ab.2015.03.034, 2015.04.
17. Sakaguchi S., Shono J., Suzuki T., Sawano S., Anderson JE, Do MK, Ohtsubo H, Mizunoya W., Sato Y., Nakamura M., Furuse, M., Yamada K., Ikeuchi Y., Tatsumi R., Implication of anti-inflammatory macrophages in regenerative moto-neuritogenesis: Promotion of myoblast migration and neural chemorepellent semaphorin 3A expression in injured muscle, INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 10.1016/j.biocel.2014.05.032, 54, 272-285, 2014.09.
18. Shono J., Sakaguchi S., Suzuki T., Do MK, Mizunoya W., Nakamura M., Sato Y., Furuse M., Yamada K., keuchi Y., Tatsumi R., Preliminary time-course study of antiinflammatory macrophage infiltration in crush-injured skeletal muscle., Anim. Sci. J., 84, 11, 744-750, 2013.12.
19. Mizunoya W., Iwamoto Y., Shirouchi B., Sato M., Komiya Y., Rahimi F., Tatsumi R., Sato Y., Nakamura M., Ikeuchi Y., Dietary Fat Influences the Expression of Contractile and Metabolic Genes in Rat Skeletal Muscle, PLOS ONE, 10.1371/journal.pone.0080152, 8, 11, 2013.11.
20. Sato Y., Do MK, Suzuki T., Ohtsubo H., Mizunoya W., Nakamura M., Furuse, M., Ikeuchi Y., Tatsumi R., Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury, ANIMAL SCIENCE JOURNAL, 10.1111/asj.12014, 84, 2, 185-189, 2013.02.
21. Suzuki T., Do MK., Sato Y., Ojima K., Hara M., Mizunoya W., Nakamura M., Furuse M., Ikeuchi Y., Anderson JE., Ryuichi Tatsumi, Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite cells in vitro toward understanding its role in modulating myogenin expression, INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 10.1016/j.biocel.2012.10.003, 45, 2, 476-482, 2013.02.
22. Do MK, Suzuki T., Borjigin G, Sato Y., Mizunoya W., Nakamura M., Ikeuchi Y., Anderson JE., Tatsumi R., Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-ss 3 in crush-injured skeletal muscle, ANIMAL SCIENCE JOURNAL, 10.1111/j.1740-0929.2012.01057.x, 83, 10, 712-717, 2012.10.
23. Do MK, Sato Y, Shimizu N, Suzuki T, Shono J, Mizunoya W, Nakamura M, Ikeuchi Y, Anderson JE, Tatsumi R., Growth factor regulation of neural chemorepellent Sema3A expression in satellite cell cultures., Am J Physiol Cell Physiol. 2011 Nov;301(5):C1270-9. Epub 2011 Aug 24., 301, 5, C1270-9, 2012.06.
24. Hara M, Tabata K, Suzuki T, Do MK, Mizunoya W, Nakamura M, Nishimura S, Tabata S, Ikeuchi Y, Sunagawa K, Anderson JE, Allen RE, Tatsumi R., Calcium influx through a possible coupling of cation channels impacts skeletal muscle satellite cell activation in response to mechanical stretch., Am J Physiol Cell Physiol. 2012 Jun;302(12):C1741-50., 2012.06.
25. Choe SK*, Nakamura M*, Ladam F, Etheridge L, Sagerström CG. *Equal Contribution, A Gal4/UAS system for conditional transgene expression in rhombomere 4 of the zebrafish hindbrain. , Dev Dyn. 2012 Jun;241(6):1125-32, 2012.06.
26. Harada A, Ohkawa Y, Ao S, Odawara J, Okada S, Azuma M, Nishiyama Y, Nakamura M, Tachibana T., Rat monoclonal antibody specific for MyoD., Hybridoma (Larchmt). 2010 Jun;29(3):255-8., 2010.06.
27. Kotani M, Harada A, Odawara J, Azuma M, Okada S, Nishiyama Y, Nakamura M, Tachibana T, Ohkawa Y., Monoclonal antibody specific for Dhx9/NDHII/RHA., Hybridoma (Larchmt). 2010 Jun;29(3):259-61., 29, 3, 2010.06.
28. Harada A, Yoshimura S, Odawara J, Azuma M, Okada S, Nakamura M, Tachibana T, Ohkawa Y., Rat monoclonal antibody specific for the chromatin remodeling factor, CHD1., Hybridoma (Larchmt). 2010 Jun;29(3):237-40, 2010.04.
29. Harada A, Yoshimura S, Odawara J, Azuma M, Okada S, Nakamura M, Tachibana T, Ohkawa Y., Generation of a rat monoclonal antibody specific for CHD2., Hybridoma (Larchmt). 2010 Apr;29(2):173-7., 2010.04.
30. Harada A, Okada S, Odawara J, Kumamaru H, Saiwai H, Aoki M, Nakamura M, Nishiyama Y, Ohkawa Y., Production of a rat monoclonal antibody specific for Myf5., Hybridoma (Larchmt). 2010 Feb;29(1):59-62., 2010.02.
31. Harada A, Okada S, Saiwai H, Aoki M, Nakamura M, Ohkawa Y., Generation of a rat monoclonal antibody specific for Pax7., Hybridoma (Larchmt). 2009 Dec;28(6):451-3., 2009.12.
32. Okada S, Harada A, Saiwai H, Nakamura M, Ohkawa Y., Generation of a rat monoclonal antibody specific for Brm., Hybridoma (Larchmt). 2009 Dec;28(6):455-8., 2009.12.
33. Ohkawa Y, Harada A, Nakamura M, Yoshimura S, Tachibana T., Production of a rat monoclonal antibody against Brg1, Hybridoma (Larchmt). 2009 Dec;28(6):463-6, 2009.12.
34. Choe SK, Lu P, Nakamura M, Lee J, Sagerström CG., Meis cofactors control HDAC and CBP accessibility at Hox-regulated promoters during zebrafish embryogenesis., Dev Cell. 2009 Oct;17(4):561-7., 2009.10.
35. Nakamura M, Choe SK, Runko AP, Gardner PD, Sagerström CG., Nlz1/Znf703 acts as a repressor of transcription., BMC Dev Biol., 2008 Nov 12;8:108, 2008.11.
36. Nakamura M., Runko AP., and Sagerstrom CG, A novel subfamily of zinc finger genes involoved in embryonic development, J. Cell. Biochem., 2004.11.
37. Nishida W, Nakamura M, Mori S, Takahashi M, Ohkawa Y, Tadokoro S, Yoshida K, Hiwada K, Hayashi K, Sobue K., A triad of serum response factor and the GATA and NK families governs the transcription of smooth and cardiac muscle genes., J Biol Chem. 2002 Mar 1;277(9):7308-17. Epub 2001 Dec 14., 2002.03.
38. Nakamura M, Nishida W, Mori S, Hiwada K, Hayashi K, Sobue K., Transcriptional activation of beta-tropomyosin mediated by serum response factor and a novel Barx homologue, Barx1b, in smooth muscle cells., J Biol Chem. 2001 May 25;276(21):18313-20., 2001.05.
39. Sun J, Tadokoro S, Imanaka T, Murakami SD, Nakamura M, Kashiwada K, Ko J, Nishida W, Sobue K., Isolation of PSD-Zip45, a novel Homer/vesl family protein containing leucine zipper motifs, from rat brain., FEBS Lett. 1998 Oct 23;437(3):304-8., 1998.10.

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