Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Kaoru Takegawa Last modified date:2024.05.12

Professor / Molecular Microbiology and Biomass Resourse Chemistry Course / Department of Bioscience and Biotechnology / Faculty of Agriculture


Papers
1. Morita Y, Takegawa K, Collins BM, Higuchi Y, Polarity-dependent expression and localization of secretory glucoamylase mRNA in filamentous fungal cells., Microbiological Research, 282, 127653 (2024), 2024.03.
2. Doi K, Mitani A, Nakakita S, Higuchi Y, Takegawa K, Characterization of novel endo-β-N-acetylglucosaminidases from intestinal Barnesiella intestinihominis that hydrolyze multi-branched complex-type N-glycans., Journal of Bioscience and Bioengineering, in press (2024), 2024.02.
3. Fukunaga T, Watanabe M, Nakamichi Y, Morita T, Higuchi Y, Maekawa H, Takegawa K, Mechanistic insights into Schizosaccharomyces pombe GT-A family protein Pvg3 in the biosynthesis of pyruvylated beta1,3-galactose of N-linked oligosaccharides., Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2023.03.002., in press (2023), 2023.08.
4. Fukunaga T, Ohashi T, Tanaka Y, Yoshimatsu T, Higuchi Y, Maekawa H, Takegawa K, Galactosylation of cell-surface glycoprotein required for hyphal growth and cell wall integrity in Schizosaccharomyces japonicus., Journal of Bioscience and Bioengineering, doi: 10.1016/j.jbiosc.2022.07.014., in press (2022), 2022.11.
5. Fukuhara R, Ogura A, Yoshinaga S, Fukunaga T, Kinoshita T, Sumiyoshi W, Higuchi Y, Tanaka K, Takegawa K., In vivo imaging of fluorescent albumin modified with pyruvylated-human-type complex oligosaccharide reveals sialylation-like biodistribution and kinetics., Bioorganic & Medicinal Chemistry, 10.1016/j.bmc.2022.116943. , 70:116943 (2022), 2022.09.
6. Bienes KM, Tautau FAP, Mitani A, Kinoshita T, Nakakita S, Higuchi Y, Takegawa K, Characterization of novel endo-β-N-acetylglucosaminidase from Bacteroides nordii that hydrolyzes multi-branched complex type N-glycans.
, Journal of Bioscience and Bioengineering, in press (2022), 2022.05.
7. Maekawa H, Jiangyan S, Takegawa K, Pereira G, SIN-like pathway kinases regulate the end of mitosis in the methylotrophic yeast Ogataea polymorpha., Cells, doi.org/10.3390/cells11091519, 11(9), 1519 (2022), 2022.05.
8. Hiasa R, Kakimoto K, Takegawa K, Higuchi Y, Involvement of AAA ATPase AipA in endocytosis depending on AoAbp1 in Aspergillus oryzae., Fungal Biology, doi: 10.1016/j.funbio.2021.11.007., 126, 149-161(2022), 2022.01.
9. Fukunaga T, Sakurai Y, Ohashi T, Higuchi Y, Maekawa H, Takegawa K, Overexpression of cell-wall GPI-anchored proteins restores cell growth of N-glycosylation-defective och1 mutants in Schizosaccharomyces pombe., Applied Microbiology and Biotechnology, doi: 10.1007/s00253-021-11649-5., 105(23), 8771-8781 (2021), 2021.11.
10. Morita Y, Katakura Y, Takegawa K, Higuchi Y, Correlative localization analysis between mRNA and EGFP-fused protein by single-molecule FISH using an egfp prove in Aspergillus oryzae., Frontiers in Fungal Biology, doi: 10.3389/ffunb.2021.72139, 2, 721398 (2021), 2021.10.
11. #Fukunaga T, Tanaka N, Furumoto T, Nakakita S, Ohashi T, Higuchi Y, Maekawa H, Takegawa K, Substrate specificities of α1,2- and α1,3-galactosyltransferases and the order of galactosylation in Schizosaccharomyces pombe., Glycobiology, doi: 10.1093/glycob/cwab028, 31(8), 1037-1045 (2021), 2021.06.
12. Tanaka N, Kagami A, Hirai K, Suzuki S, Matsuura S, Fukunaga T, Tabuchi M, Takegawa K., The fission yeast gmn2+ gene encodes an ERD1 homologue of Saccharomyces cerevisiae required for protein glycosylation and retention of luminal endoplasmic reticulum proteins., The Journal of General and Applied Microbiology, doi: 10.2323/jgam.2020.07.002., 67, 67-76 (2021), 2021.03.
13. Kawano-Kawada M, Ueda T, Mori H, Ichimura H, Takegawa K, Sekito T, Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids in Schizosaccharomyces pombe., Biochimica et Biophysica Acta-Biomembranes, doi: 10.1016/j.bbamem.2020.183507., 1863(2): 183507 (2021), 2021.02.
14. Matsunaga E, Tanaka Y, Toyota S, Yamada H, Oka T, Higuchi Y, Takegawa K, Identification and characterization of β-D-galactofuranosidases from Aspergillus nidulans and Aspergillus fumigatus., Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2020.09.006., 131(1), 1-7 (2021), 2021.01.
15. Ohashi T, Hegi S, Fukunaga T, Hosomi A, Takegawa K, Golgi localization of glycosyltransferase requires Gpp74p in Schizosaccharomyces pombe., Applied Microbiology and Biotechnology, 104(20), 8897-8909 (2020), 2020.10, ゴルジ体局在グリコシルトランスフェラーゼの大部分は、N末端に小さな細胞質ゾルの尾を持つII型膜タンパク質である。これらのグリコシルトランスフェラーゼをゴルジに局在化させるためのいくつかのメカニズムが提案されている。 出芽酵母Saccharomyces cerevisiaeでは、ホスファチジルイノシトール-4-リン酸結合タンパク質ScVps74pが、ゴルジ糖転移酵素の細胞質ゾル尾部と相互作用し、その局在化に寄与する。本論文では、同様のメカニズムが分裂酵母Schizosaccharomycespombeで機能するかどうかを調査した。まず、S。cerevisiaeVps74pのS.pombeホモログをコードする遺伝子であるgpp74 +(GPP34ドメインを含むVps74ホモログタンパク質)を同定した。 gpp74 +遺伝子の削除は、3つのゴルジグリコシルトランスフェラーゼ、SpOch1p、SpMnn9p、およびSpOmh1pの液胞へのミスソーティングをもたらしたが、SpAnp1pではなく、Gpp74pがいくつかのグリコシルトランスフェラーゼをゴルジ装置にターゲティングするために必要であることがわかった。ゴルジ装置と細胞質ゾルの両方に局在するN末端GFPタグを持つGpp74p。 Gpp74pのゴルジ局在は、ホスファチジルイノシトール4-キナーゼSpPik1pに依存していた。 SpOch1pおよびSpMnn9pの細胞質側テールにおける疎水性および塩基性アミノ酸の部位特異的変異誘発は、液胞へのそれらのミスソーティングを引き起こし、これらのサイトゾルN末端残基がゴルジ局在化に重要であることを示した。S. pombegpp74Δ細胞ではタンパク質のグリコシル化に顕著な変化は観察されなかった。これはおそらく、一部のSpOch1pおよびSpMnn9pの残存ゴルジ局在化によるものと考えられた。以上の結果から、Gpp74p依存性とGpp74p非依存性の両方のメカニズムがS.pombeグリコシルトランスフェラーゼのゴルジ局在化に関与していることを明らかにした。.
16. Higuchi Y, Takegawa K, Single-molecule FISH reveals subcellular localization of α-amylase and actin mRNAs in the filamentous fungus Aspergillus oryzae., Frontiers in Microbiology, 10.3389/fmicb.2020.578862, 11:578862 (2020), 2020.10.
17. Morita Y, Kikumatsu F, Higuchi Y, Katakura Y, Takegawa K, Characterization and functional analysis of ERAD-related AAA+ ATPase Cdc48 in Aspergillus oryzae., Fungal Biology, 10.1016/j.funbio.2020.06.004, 124(9), 801-813 (2020), 2020.09.
18. Ohashi T, Tanaka T, Tanaka N, Takegawa K., SpMnn9p and SpAnp1p form protein complex involved in mannan backbone synthesis in the fission yeast Schizosaccharomyces pombe., Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2020.06.003., in press (2020), 2020.08.
19. Papali Tautau FA, Izumi M, Matsunaga E, Higuchi Y, Takegawa K., Microbial α-L-rhamnosidases show a dual L-rhamnose and L-mannose hydrolyzing activity., Journal of Applied Glycoscience, 10.5458/jag.jag.JAG-2020_0005, 67, 87-93 (2020), 2020.07.
20. Fukunaga T, Tanaka N, Furumoto T, Nakakita S, Ohashi T, Higuchi Y, Maekawa H, Takegawa K, Characterization of N- and O-linked galactosylated oligosaccharides from fission yeast species., Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2020.03.008., 130(2), 128-136 (2020), 2020.04.
21. Li Q, Higuchi Y, Tanabe K, Katakura Y, Takegawa K, Secretory production of N-glycan-deleted glycoprotein in Aspergillus oryzae., Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2019.12.006. , 129(5), 573-580 (2020), 2020.02.
22. Shen L, Viljoen A, Villaume S, Maju J, Chene L, Mery A, Fabre E, Takegawa K, Lowary TL, Vincent SP, Kremer L, Guerardel Y, Mariller C, The endogenous galactofuranosidase GlfH1 hydrolyzes mycobacterial arabinogalactan., Journal of Biological Chemistry, 10.1074/jbc.RA119.011817., in press (2020), 2020.02.
23. Chihara Y, Tanaka Y, Izumi M, Hagiwara D, Watanabe A, Takegawa K, Kamei K, Shibata N, Ohta K, Oka T, Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Gal-actomannans within the Invasive Pathogen Aspergillus fumigatus., mSphere, in press (2020), 2020.01.
24. Ashida H, Fujimoto T, Kurihara S, Nakamura M, Komeno M, Huang Y, Katayama T, Kinoshita T, Takegawa K, 1,6-alpha-Fucosidases from Bifidobacterium longum subsp. Infantis ATCC15697 involved in the degradation of core-fucosylated N-glycan., Journal of Applied Glycoiscience, 67, 23-29 (2020), 2020.01.
25. Seki H, Huang Y, Arakawa T, Yamada C, Kinoshita T, Iwamoto, Higuchi Y, Takegawa K, Fushinobu S, Structural basis for specific cleavage of core-fucosylated N-glycans by endo-beta-N-acetylglu-cosaminidase from Cordyceps militaris., Journal of Biological Chemistry, 10.1074/jbc.RA119.010842., 294(45), 17143-17154 (2019), 2019.10, ENGaseは糖タンパク質のアスパラギン(Asn)残基に結合した糖鎖を切断して遊離する酵素である。これまでいくつかのENGaseの立体構造が報告されており、遊離する糖鎖部分の結合の様子については知見があった。しかし、切断点よりタンパク質に近い部分、特にAsnに結合したN-アセチルグルコサミン(GlcNAc)と、しばしばそのGlcNAcに付加している「コアフコース」に対して、ENGaseがどのように結合して見分けているかは全くわかっていなかった。本論文ではX線結晶構造解析により、「冬虫夏草」の1種であるサナギタケから得られた新規なENGaseの立体構造を決定し、コアフコース、GlcNAc、Asnを認識して切断する様子を明らかにした。コアフコースはヒトなどの哺乳動物の糖鎖に付加されていることが多く、ENGaseは抗体などの糖タンパク質の糖鎖を均一なものにすることができるため、本研究の成果は、近年承認数が急増しているバイオ医薬品(抗体医薬)の性能向上および品質管理などへの応用が期待される。.
26. Senicar M, Legentil L, Ferrieres V, Eliseeva SV, Petoud S, Takegawa K, Lafite P, Daniellou R, Galactofuranosidase from JHA19 Streptomyces sp.: subcloning and biochemical characteriza-tion., Carbohydrate Research, 10.1016/j.carres.2019.05.011., 480, 35-41 (2019), 2019.06.
27. Tominaga A, Higuchi Y, Mori H, Akai M, Suyama A, Yamada N, Takegawa K, Catechol O-methyltransferase homologs in Schizosaccharomyces pombe are response fac-tors to alkaline and salt stress., Applied Microbiology and Biotechnology, 10.1007/s00253-019-09858-0., 103(12), 4881-4887 (2019), 2019.05.
28. Ota R, Okamoto Y, Vavricha CJ, Oka T, Matasunaga E, Takegawa K, Kiyota H, Izumi M, Chemo-enzymatic synthesis of p-nitrophenyl β-D-galactofuranosyl disaccharides from Aspergillus sp. fungal-type galactomannan, Carbohydrate Research, 473, 99-103 (2019), 2019.02.
29. Higuchi Y, Matsufuji H, Tanuma M, Arakawa T, Mori K, Yamada C, Shofia R, Matsunaga E, Tashiro K, Fushinobu S, Takegawa K, Identification and characterization of a novel β-D-galactosidase that releases pyruvylated ga-lactose., Scientific Reports, 10.1038/s41598-018-30508-4, 8(1), 12013 (2018), 2018.09.
30. Higuchi Y, Matsufuji H, Mori K, Matsunaga E, Tashiro K, Takegawa K, Draft genome sequence of Bacillus sp. HMA207, a strain that exhibits β-D-galactosidase activ-ity to release pyruvylated galactose., Microbiology Resource Announcements, 10.1128/MRA.01169-18, 7(10), e01169-18 (2018)., 2018.09.
31. Kimura Y, Yamamoto Y, Kajimoto S, Sakai A, Takegawa K, Substrate specificity of Nudix hydrolases from Myxococcus xanthus., Journal of General and Applied Microbiology, 10.2323/jgam.2017.07.001, 64(2), 94-98 (2018), 2018.05.
32. Kamatani S, Takegawa K, Kimura Y, Catalytic activity profile of polyphosphate kinase 1 from Myxococcus xanthus., Current Microbiology, 10.1007/s00284-017-1391-y., 75(4), 379-385 (2018), 2018.04.
33. Kajiwara Y, Mori K, Tashiro K, Higuchi Y, Takegawa K, Takashita H, Genomic sequence of Saccharomyces cerevisiae BAW-6, a yeast strain opt imal for brewing barley shochu., Genome Announcements, 10.1128/genomeA.00228-18, 6(14), e00228-18 (2018), 2018.04.
34. Sugiura A, Nguyen LC, Shamim HM, Iida N, Nakamura Y, Iida T, Nakase M, Takegawa K, Senda M, Jida S, Ueno M, Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1., Biochemistry, Biophysics and Research Communications, 10.1016/j.bbrc.2018.02.001., 496(4), 1284-1290 (2018), 2018.02.
35. Huang Y, Higuchi Y, Mori K, Tashiro K, Takegawa K, Draft genome sequence of Sphingobacterium sp. HMA12, a strain that holds endo-β-N-acetylglucosaminidases specifically hydrolyzing fucose-containing oligosaccharides., Genome Announcements, 10.1128/genomeA.01525-17., 6(8), e01525-17 (2018), 2018.02.
36. Yujiro Higuchi, Hikari Mori, Takeo Kubota, Takegawa K, Analysis of alkaline stress response mediated by iron and copper intake in Schizosaccharomyces pombe., Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2017.08.008., 125(1), 92-98 (2018), 2018.01.
37. Huang Y, Higuchi Y, Kinoshita T, Mitani A, Eshima Y, Takegawa K, Characterization of novel endo-β-N-acetylglucosaminidases from Sphingobacterium species, Beauveria bassiana and Cordyceps militaris that specifically hydrolyze fucose-containing oli-gosaccharides and human IgG., Scientific Reports, 8(1):248 (2018), 2018.01.
38. Tsukimura W, Kurogochi M, Mori M, Osumi K, Matsuda A, Takegawa K, Furukawa K, Shirai T, Preparation and biological activities of anti-HER2 monoclonal antibodies with fully core-fucosylated homogeneous bi-antennary complex-type glycans., Bioscience, Biotechnology, and Biochemistry, 10.1016/j.jbiosc.2017.08.008., 81(12), 2353-2359 (2017), 2017.12.
39. Togo Y, Higuchi Y, Katakura Y, Takegawa K, Early endosome motility mediates α-amylase production and cell differentiation in Aspergillus oryzae., Scientific Reports, 10.1038/s41598-017-16163-1., 7(1): 15757 (2017), 2017.11.
40. Yamamoto K, Tran T, Takegawa K, Kaneko Y, Maekawa H, Regulation of the mating type switching by the mating type genes and RME1 in Ogataea polymorpha., Scientific Reports, 10.1038/s41598-017-16284-7., 7(1): 16318 (2017), 2017.11.
41. HeeSu Kwon, Kouhei Kawaguchi, Takashi Kukuma, Kaoru Takegawa, Katsuhiko Kitamoto, Yujiro Higuchi, Analysis of acyl-CoA binding protein in Aspergillus oryzae that undergoes unconventional secretion. , Biochemical and Biophysical Research Communications, in press (2017), 2017.10.
42. Suyama A, Higuchi Y, Urushihara M, Maeda Y, Takegawa K, Production of 3-hydroxypropionic acid via malonyl-CoA pathway using recombinant fission yeast strains., Journal of Bioscience and Bioengineering, 10.1016/j.jbiosc.2017.04.015, 124, 392-399 (2017), 2017.08.
43. Katafuchi Y, Li Q, Tanaka Y, Kawamitsu Y, Shinozuka S, Izumi M, Ekino K, Mizuki K, Takegawa K, Shibata N, Goto M, Nomura Y, Ohta K, Oka T, GfsA is a β1,5-galactofuranosyltransferase involved in the biosynthesis of the galactofuran side chain of fungal-type galactomannan in Aspergillus fumigatus, Glycobiology, 10.1093/glycob/cwx028, 27(6), 568-581 (2017), 2017.06.
44. Matsunaga E, Higuchi Y, Mori K, Tashiro K, Takegawa K, Draft genome sequence of Streptomyces sp. JHA26, a strain that harbors a PA14 domain-containing β-D-galactofuranosidase, Genome Announcements, 10.1128/genomeA.00190-17, 5(15), pii: e00190-17, 2017.06.
45. Matsunaga E, Higuchi Y, Mori T, Yairo N, Toyota S, Oka T, Tashiro K, Takegawa K, Characterization of PA14 domain-containing galactofuranose-specific β-D-galactofuranosidase from Streptomyces sp., Bioscience, Biotechnology and Biotechemistry, 10.1080/09168451.2017.1300518., 81(7), 1314-1319 (2017), 2017.05.
46. Higuchi Y, Eshima Y, Huang Y, Kinoshita T, Sumiyoshi W, Nakakita S, Takegawa K, Highly efficient transglycosylation of sialo-complex-type oligosaccharide using Coprinopsis cinerea endoglycosidase and sugar oxazoline., Biotechnology Letters, 10.1016/j.bbrc.2016.10.018., 39(1), 8-12 (2017), 2017.01.
47. Kawano-Kawada M, Chardwiriyapreecha S, Manabe K, Sekito T, Akiyama K, Takegawa K, Kakinuma Y, The amino-terminal hydrophilic region of the vacuolar transporter Avt3p is dispensable for the vacuolar amino acid compartmentalization of Schizosaccahromyces pombe., Bioscience, Biotechnology and Biochemistry, 80(12), 2291-2297 (2016), 2016.12.
48. Kawaguchi K, KikumaT, Yujiro Higuchi, Takegawa K, Kitamoto K, Subcellular localization of acyl-CoA binding protein in Aspergillus oryzae is regulated by autophagy machinery., Biochemical and Biophysical Research Communications, 480(1), 8-12 (2016), 2016.12.
49. Oka M, Takegawa K, Kimura Y, Lysyl-tRNA synthetase from Myxococcus xanthus catalyzes the formation of diadenosine penta- and hexaphsphates from denosine tetraphosphate., Archives of Biochemistry and Biophysics, 604, 152-158 (2016), 2016.08.
50. Higuchi Y, Yoshinaga S, Yoritsune K, Tateno H, Hirabayashi J, Nakakita S, Kanekiyo M, Kakuta Y, Takegawa K, A rationally engineered yeast pyruvyltransferase Pvg1p introduces sialylation-like properties in neo-human-type complex oligosaccharide., Scientific Reports, 6:26349 (2016), 2016.05, 分裂酵母の糖鎖には酸性糖としてガラクトース残基にピルビン酸が付加される。我々はこれまでに糖鎖へのピルビン酸の付加に関与する酵素としてPvg1/ピルビン酸転移酵素を同定していた。そこで本論文では、Pvg1タンパク質をX線結晶構造から解析を行い、ピルビン酸転移酵素において初めて立体構造を明らかにすることができた。得られた立体構造から、本酵素活性に重要なアミノ酸を特定し、そのアミノ酸変異体を作成することで、これまで全く報告のない新奇なアスパラギン結合型糖鎖を酵素合成することができた。シアル酸の代わりにピルビン酸が付加された本糖鎖は、レクチンマイクロアレイ解析から、シアル酸がα2,6-結合でガラクトースに付加した構造と類似していることを明らかにした。.
51. Higuchi Y, Mori K, Suyama A, Huang Y, Tashiro K, Kuhara S, Takegawa K, Draft genome sequence of Bacillus clausii AKU0647, a strain that produces endo-β-N-acetylglucosaminidase A, Genome Announcements, e00315-16 (2016), 2016.04.
52. Matsunaga E, Higuchi Y, Mori K, Tashiro K, Kuhara S, Takegawa K, Draft genome sequence of Streptomyces sp. JHA19, a strain that possesses β-D-galactofuranosidase activity., Genome Announcements, 3(5):e01171-01175 (2015), 2015.10.
53. Matsunaga E, Higuchi Y, Mori K, Yairo N, Oka T, Shinohara S, Tashiro K, Izumi M, Kuhara S, Takegawa K, Identification and characterization of a novel galactofuranose-specific β-D-galactofuranosidase from Streptomyces species., PLOS ONE, 10(9):e0137230 (2015), 2015.09.
54. Oka M, Takegawa K, Kimura Y, Enzymatic characterization of a class II lysyl-rRNA synthetase, LysS, from Myxococcus xanthus., Archives of Biochemistry and Biophysics, 579, 33-39 (2015), 2015.07.
55. Eshima Y, Higuchi Y, Kinoshita T, Nakakita S, Takegawa K, Transglycosylation activity of glycosynthase mutants of endo-β-N-acetylglucosaminidase from Coprinopsis cinerea, PLOS ONE, 10(7):e0132859 (2015), 2015.07.
56. Kato T, Shirakawa Y, Takegawa K, Kimura Y, Functional analysis of conserved motifs in a bacterial tyrosine kinase, BtkB, from Mycococcus xanthus., Journal of Biochemistry, 158(5), 385-392 (2015), 2015.06.
57. Chardwiriyapreecha S, Manabe S, Iwaki T, Kawano-kawada M, Sekito T, Lnprom S, Akiyama K, Takegawa K, Kakinuma Y, Functional expression and characterization of Schizosaccharomyces pombe Avt3p as a vacuolar amino acid exporter in Saccharomyces cerevisiae., PLOS ONE, 10(6):e0130542 (2015), 2015.06.
58. Tsukamoto Y, Kagiwada S, Shimazu S, Takegawa K, Noguchi T, Miyamoto M, Coordinated regulations by two VPS9 domain-containing guanine-nucleotide exchange factors., Biochem. Biophys. Res. Commun., 458(4), 802-809 (2015), 2015.03.
59. Hosomi A, Higuchi Y, Yagi S, Takegawa K, Vsl1p cooperates with Fsv1p for vacuolar protein transport and homotypic fusion in Schizosaccharomyces pombe., Microbiology, 161(1), 89-98 (2015), 2015.01.
60. Yoritsune KI, Higuchi Y, Matsuzawa T, Takegawa K, Functional analysis of putative phosphoenolpyruvate transporters localized to the Golgi apparatus in Schizosaccharomyces pombe, FEMS Yeast Research, 14, 1101-1109 (2014), 2014.12.
61. Sasaki M, Takegawa K, Kimura Y, Enzymatic characteristics of an ApaH-like phosphatase, PrpA, and a diadenosine tetraphosphate hydrolase, ApaH, from Myxococcus xanthus., FEBS Letters, 588(18) 3395-3402 (2014), 2014.09.
62. Okamoto R, Takegawa K, Kimura Y, Regulation of eukaryotic-like protein kinase activity of DspA from Myxococcus xanthus by autophosphorylation., Journal of Biochemistry, 155(2), 99-106 (2014), 2014.02.
63. Futagami T, Seto K, Kajiwara Y, Takashita H, Omori T, Takegawa K, Goto M, The purative stress sensor protein MtlA is required for conidia formation, cell wall stress tolerance, and cell wall integrity in Aspergillus nidulans., Bioscience, Biotechnology, and Biochemistry, 78(2), 326-335 (2014), 2014.01.
64. Komachi Y, Hatakeyama S, Motomatsu H, Futagami T, Kinzjakina K, Sorbado P, Ekino K, Takegawa K, Goto M, Nomura Y, Oka T, gfsA encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of O-glycan in Aspergillus nidulans and A. fumigatus., Molecular Microbiology, 90(5), 1054-1073 (2013), 2013.12, アスペルギルス属糸状菌は糖鎖中にガラクトフラノースが存在することは古くから知られているが、これまでガラクトフラノース転移酵素遺伝子に関する報告は全く無い。そこでAspergillus nidulansゲノムに存在する仮想糖転移酵素遺伝子を破壊した株を作成し、解析を行ったところ、GfsAと命名した遺伝子がガラクトフラノース転移酵素をコードしていることを明らかにした。実験の結果、GfsAはβ1,5-かβ1,6-ガラクトフラノース転移酵素活性を有しており、O-結合型糖鎖糖タンパク質にUDP-ガラクトースを基質として転移することがわかった。GfsAはゴルジ体に局在していた。GfsAと相同性の高い遺伝子はAspergillus fumigatusにも存在していることもわかった。.
65. Nakase Y, Nakase M, Kashiwazaki J, Murai T, Otsubo Y, Mabuchi I, Yamamoto M, Takegawa K, Mastumoto T, The fission yeast β-arrestin-like protein Any1 is involved in TSC-Rheb signaling and the regulation of amino acid transporters., Journal of Cell Science, 126(17), 3972-3981 (2013), 2013.09, Rheb GTPaseであるTsc1-Tsc2複合体は細胞外の環境に応答して細胞成長調節において重要な役割を果たしている。分裂酵母においてTsc1-Tsc2複合体は細胞周期の進行やアミノ酸の取り込みなどを調節することが報告されていた。我々はE3ユビキチンリガーゼであるPub1とβ-アレスチン様タンパクであるAny1が細胞膜アミノ酸トランスポーターであるAat1の局在を制御していることを見出した。またPub1とAny1が分裂酵母細胞内で相互作用していることからTSC-Rhebシグナル経路がPub1やAny1を通じて細胞膜のアミノ酸トランスポーターの局在を制御していることを明らかにした。.
66. Pongharoen P, Kawano-Kawada M, Iwaki T, Sugimoto N, Sekito T, Akiyama K, Takegawa K, Kakinuma Y, Functional expression of Schizosaccharomyces pombe Vba2p in vacuolar membrane of Saccharomyces cerevisiae., Bioscience, Biotechnology, and Biochemistry, 77(9), 1988-1990 (2013)., 2013.09.
67. Matsuzawa T, Tohda H, Takegawa K, Ethanol-inducible gene expression using gld1+ promoter in the fission yeast Schizosaccharomyces pombe., Applied Microbiology and Biotechnology, 97(15), 6835-6843 (2013), 2013.08.
68. Tashiro S, Futagami T, Wada S, Kajiwara Y, Takashita H, Omori T, Takahashi T, Yamada O, Takegawa K, Goto M, Construction of a ligD disruptant for efficient gene targeting in white koji mold, Aspergillus kawachii., Journal of General and Applied Microbiology, 59, 257-260 (2013), 2013.07.
69. Matsuzawa T, Hara F, Tanaka N, Tohda H, Takegawa K, ght2+ is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe., Applied Microbiology and Biotechnology , 97(11), 4957-4964 (2013), 2013.05.
70. Sasaki M, Kumagai H, Takegawa K, Tohda H, Characterization of genome-reduced fission yeast strains., Nucleic Acid Research, 41(10), 5382-5399 (2013)., 2013.05, 分裂酵母はモデル真核生物の中で最も遺伝子数の少ない生物である。我々は通常の培養条件で生育の低下しない分裂酵母の大規模遺伝子削除株の取得を試みた。最終的に223遺伝子(657.3kb)を同一株で削除した株の取得に成功した。本株で異種タンパク質であるGFPやヒト成長ホルモンを発現させたところ、野生株よりもそれぞれ約1.7倍、1.8倍高い生産能を示すことがわかり、分裂酵母の大規模遺伝子削除株は物質生産への応用にも使用できる可能性が示唆された。.
71. Yoritsune Ken-ichi, Matsuzawa Tomohiko, Ohashi Takao, Takegawa Kaoru, The fission yeast Pvg1p shows galactose-specific pyruvyltransferase activity., FEBS Letters, 587, 7, 917-921, 2013.03.
72. Matsuzawa T, Kageyama Y, Ooishi K, Kawamukai M, Takegawa K, The zinc finger protein Gsf1 regulates Gsf2-dependent flocculation in fission yeast., FEMS Yeast Research, 13(3), 259-266, 2013.03.
73. Mori Y, Maeda M, Takegawa K, and Kimura Y, A tyrosine phosphatase, PhpA, of Myxococcus xanthus is involved in the production of exopolysaccharide., Microbiology, 158(10), 2546-2555 (2012), 2012.11.
74. Ohashi T, Fujiyama K, Takegawa K, Identification of novel α1,3-galactosyltransferase and elimination of α-galactose-containing glycans by disruption of multiple α-galactosyltransferase genes in Schizosaccharomyces pombe., Journal of Biological Chemistry, 287(46), 38866-38875 (2012), 2012.11, 分裂酵母の糖鎖中には多量のガラクトースが含まれている。糖鎖構造解析の結果、ガラクトース残基はα1,2-およびα1,3-結合で付加している。分裂酵母ゲノム中に存在する全てのα1,2-ガラクトース転移酵素遺伝子を破壊した株を作成したところ、まだα1,3-結合のガラクトースが残っていることを見出した。そこでさらにゲノムを解析したところ、仮想糖転移酵素が3遺伝子存在することがわかり、これらを破壊した10重破壊株を作成したところ、糖鎖のガラクトース残基は完全に欠失することを明らかにした。また大腸菌で生産させたこれらの遺伝子産物が実際にα1,3-ガラクトース転移活性を示すこともわかった。以上の結果から分裂酵母に存在する新規α1,3-ガラクトース転移酵素の諸性質を明らかにすることができた。.
75. Mori Y, Takegawa K, and Kimura Y, Function analysis of conserved amino acid residues in a Mn2+-dependent protein phosphatase, Pph3, from Myxococcus xanthus., Journal of Biochemistry, 152(3), 269-274 (2012), 2012.09.
76. Matsuzawa T, Hara F, Tohda H, Uemura H, and Takegawa K, Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe., Applied Microbiology and Biotechnology, 95(2), 441-449 (2012), 2012.07.
77. Nakase M, Tani M, and Takegawa K, Expression of budding yeast IPT1 gene produces mannosyldiinositol phosphorylceramide in fission yeast and inhibits cell growth., Microbiology, 158(5), 1219-1228 (2012), 2012.05.
78. Nakase M, Tsukamoto Y, Hosomi A, Tsuji H, Matsuda T, Miyamoto M, and Takgawa K, CUE domain-containing protein Vps901 is required for vacuolar protein transport in Schizosaccharomyces pombe., Bioscience, Biotechnology, and Biochemistry, 76(4), 652-659 (2012), 2012.04.
79. Matsuzawa T, Mori K, Kadowaki T, Shimada M, Tashiro K, Kuhara S, Inagawa H, Soma G, and Takegawa K, Genome sequence of the Pantoea agglomerans strain IG1., Journal of Bacteriology, 194(5), 1258-1259 (2012), 2012.03.
80. Nakase M, Nakase Y, Chardwiriyapreecha S, Kakinuma Y, Matsumoto T, and Takegawa K, Intracellular trafficking and ubiquitination of the Schizosaccharomyces pombe amino acid pearmease Aat1p., Microbiology, 158(3), 659-673 (2012), 2012.03.
81. Kitamura K, Nakase M, Tohda H, and Takegawa K, The ubiquitin ligase Ubr11 is essential for the oligopeptide utilization in fission yeast Schizosaccharomyces pombe., Eukaryotic Cell, 11(3), 302-310 (2012), 2012.03.
82. Matsuzawa T, Fujita Y, Tohda H, and Takegawa K, Snf1-like protein kinase Ssp2 regulates glucose derepression in Schizosaccharomyces pombe., Eukaryotic Cell, 11(2), 159-167 (2012), 2012.02.
83. Matsuzawa T, Yoritsune K, and Takegawa K, MADS box transcription factor Mbx2/Pvg4 regulates invasive growth and flocculation by inducing gsf2+ expression in fission yeast., Eukaryotic Cell, 11(2), 151-158 (2012) , 2012.02.
84. Mukaiyama H, Kodera M, Tanaka N, Takegawa, K., N-glycans are not required for the efficient degradation of the mutant Saccharomyces cerevisiae CPY* in Schizosaccharomyces pombe., Applied Microbiology and Biotechnology, 93(4), 1609-1618 (2012), 2012.02.
85. Matsuzawa T, Morita T, Tanaka N, Tohda H, Takegawa K, Identification of galactose-specific flocculin essential for nonsexual flocculation and hyphal growth in Schizosaccharomyces pombe., Molecular Microbiology, 82(6), 1531-1544 (2011), 2011.12, 分裂酵母 Schizosaccharomyces pombeはガラクトース特異的な非性的凝集を引き起こすことは知られていたが、その分子機構については知られていなかった。我々は出芽酵母の非性的凝集を誘導するFLO8遺伝子を分裂酵母細胞内で発現させると凝集することを見いだした。そこでこの株と野生株の遺伝子発現をマイクロアレイ分析により比較して1つの遺伝子(SPCC1742.01)が FLO8発現株で上昇していることを明らかにした。本遺伝子を破壊すると凝集変異株の凝集が消失することから本遺伝子産物が、分裂酵母のガラクトース特異的な凝集素であることを同定した。また本遺伝子産物は分裂酵母の偽菌糸形成過程にも必須であることを明らかにした。.
86. Futagami T, Mori K, Yamashita A, Wada S, Kajiwara Y, Takashita H, Omori T, Takegawa K, Tashiro K, Kuhara S, and Goto M, Genome sequence of the white Koji mold, Aspergillus kawachii IFO4308 used for brewing the Japanese distilled spirit, Shochu., Eukaryotic Cell , 10(11) 1586-1587 (2011), 2011.11.
87. Hosomi A, Nakase M, Takegawa K, Schizosaccharomyces pombe Pep12p is required for vacuolar protein transport and vacuolar homotypic fusion., Journal of Bioscience and Bioengineering, 112(4), 309-314 (2011), 2011.10.
88. Kimura Y, Yamashita S, Mori Y, Kitajima Y, and Takegawa K, A Myxococcus xanthus bacterial tyrosine kinase, BtkA, is required for the formation of mature spores., Journal of Bacteriology, 193(20), 5853-5857 (2011), 2011.10.
89. Kimura Y, Mori Y, Ina Y, Takegawa K, Enzymatic and functional analysis of a protein phosphatase, Pph3, from Myxococcus xanthus., Journal of Bacteriology, 193(10), 2657-2661 (2011), 2011.05.
90. Mukaiyama H, Iwaki T, Idiris A, Takegawa K, Analysis of processing and maturation mechanism of carboxypeptidase Y and alkaline phosphatase in Schizosaccharomyces pombe., Applied Microbiology and Biotechnology , 90(1), 203-213 (2011), 2011.04.
91. Ohashi T, Nakakita S, Sumiyoshi W, Yamada N, Ikeda Y, Tanaka N, Takegawa K, Structural analysis of α1,3-linked galactose-containing oligosaccharides in Schizosaccharomyces pombe mutants harboring single and multiple α-galactosyltransferase genes disruptions, Glycobiology, 21(3), 340-351 (2011), 2011.03.
92. Sugimoto N, Iwaki T, Chardwiriyapreecha S, Shimazu M, Sekito T, Takegawa K, Kakinuma Y, Atg22p, a vacuolar membrane protein involved in amino acid compartmentalization of Schizosaccharomyces pombe., Bioscience, Biotechnology, and Biochemistry, 75(2), 385-387 (2011), 2011.03.
93. Matsuzawa T, Fujita Y, Tanaka N, Tohda H, Itadani A, Takegawa K, New insights into galactose metabolism by Schizosaccharomyces pombe: isolation of a galactose-assimilating mutant., Journal of Bioscience and Bioengineering, 111(2), 158-166 (2011), 2011.02.
94. Hirano Y, Matsuzawa T, Takegawa K, Sato MH, Loss-of- and gain-of-function mutations in FAB1A/B impair endomembrane homeostasis, conferring pleiotropic developmental abnormalities in Arabidopsis., Plant Physiology, 155(2), 797-807 (2011), 2011.02, 真核細胞において、ホスファチジルイノシトール3,5-キナーゼはFab1/PIKfyveにより合成され、PI(3,5)P2は液胞・リソソームの機能に重要である。Arabidopsis由来のFAB1A/Bを分裂酵母のfab1欠損株において発現させたところ、液胞形態異常を相補できることがわかった。またGFPを融合したFAB1A/BはArabidopsis細胞内でエンドソーム付近に局在していた。さらにFAB1A/Bの発現を抑制したところ、液胞の酸性化やエンドサイトーシスに異常を示すことを明らかにした。以上の結果からArabidopsisのFAB1A/BもPI3,5-キナーゼ活性を有しており、Arabidopsisにおいても液胞の機能に重要であることがわかった。.
95. Morita T, Ito E, Kitamoto HK, Fukuoka T, Imura T, Takegawa K, Kitamoto D, Identification of a gene PaEMT1 for biosynthesis of mannosylerythritol lipids in the basidiomycetous yeast Pseudozyma antarctica., Yeast, 27(11), 905-917 (2010), 2010.11.
96. Ohashi T, Nakakita S, Sumiyoshi W, Takegawa K, Production of heterologous glycoproteins by a glycosylation-defective alg3och1 mutant of Schizosaccharomyces pombe., Journal of Biotechnology, 150(3), 348-356 (2010), 2010.11.
97. Sugimoto N, Iwaki T, Chardwiriyapreecha M, Shimazu S, Sekito T, Takegawa K, Kakinuma Y, Vba2p: A Vacuolar Membrane Protein Involving in Basic Amino Acid Transport in Schizosaccharomyces pombe, Bioscience, Biotechnology, and Biochemistry, 74(10), 2166-2169 (2010), 2010.10.
98. Chardwiriyapreecha S, Mukaiyama H, Sekito T, Iwaki T, Takegawa K, Kakinuma Y, Avt5p is required for vacuolar uptake of amino acids in the fission yeast Schizosaccharomyces pombe., FEBS Letters, 584(11), 2339-2345 (2010), 2010.06.
99. Matsuzawa T, Ohashi T, Hosomi A, Tanaka N, Tohda H, Takegawa K, The gld1+ gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe., Applied Microbiology and Biotechnology, 87(2), 715-727 (2010), 2010.06.
100. Nakase M, Tani M, Morita T, Kitamoto HK, Kashiwazaki J, Nakamura T, Hosomi A, Tanaka N, Takegawa K, Mannosylinositol phosphorylceramide is a main phospholipid component and required for proper localization of plasma membrane proteins in Schizosaccharomyces pombe., Journal of Cell Science, 123(9), 1578-1587 (2010), 2010.05, 分裂酵母のゲノムには出芽酵母IPT1遺伝子と相同性の高い遺伝子が存在せず、脂質分析の結果、分裂酵母の主要なスフィンゴ糖脂質はIPCとMIPCのみであることがわかった。分裂酵母のMIPC合成に関与するマンノース転移酵素のホモログは3つ(Imt1-3)存在し、3重遺伝子破壊株ではMIPCが全く合成されないことが明らかになった。MIPC欠損株では生育遅延、細胞形態異常、薬剤感受性、タンパク質の局在異常やエンドサイトーシス異常などの多様な表現型を示すことから、分裂酵母のMIPCは様々なメカニズムに関与する重要な因子であり、分裂酵母には特有のマンノース認識機構が存在する可能性が示唆された。さらに、MIPC欠損株では細胞膜に局在するアミノ酸パーミアーゼ(AAP)の選別輸送に異常が生じることがわかった。.
101. Mukaiyama H, Nakase M, Nakamura T, Kakinuma Y, Takegawa K, Autophagy in the fission yeast Schizosaccharomyces pombe., FEBS Letters, 584(7), 1327-1334 (2010), 2010.04.
102. Hasehira K, Nakakita S, Miyanishi N, Sumiyoshi W, Hayashi S, Takegawa K, Hirabayashi J, A comprehensive HPLC analytical system for the identification and quantification of hexoses that employs 2-aminobenzamide coupling., Journal of Biochemistry, 147(4), 501-509 (2010), 2010.04.
103. Onishi M, Koga T, Hirata A, Nakamura T, Asakawa H, Shimoda C, Bahler J, Wu J-Q, Takegawa K, Tachikawa H, Pringle JR, Fukui Y, Role of septins in the orientation of forespore-membrane extension during sporulation in fission yeast., Molecular and Cellular Biology, 30(8), 2057-2074 (2010), 2010.04, 酵母の胞子形成過程において、前胞子膜(FSM)はスピンドルポールボディーにおいて胞子縁を形成するのに寄与している。この過程におけるセプチンの機能を分裂酵母を用いて解析を行った。1倍体の性的凝集の前にはSpn1-4のセプチンが、また胞子形成時には別のセプチン(Spn2, Spn5, Spn6, Spn7)が機能すること、またこれらのセプチンはin vitroでは複合体を形成していることを明らかにした。Spn2とSpn7はホスファチジルイノシトール4-リン酸(PI4-P)と結合すること、またPI4-Pが前胞子膜に多く存在することを明らかにした。.
104. Mukaiyama H, Tohda H, Takegawa K, Overexpression of protein disulfide isomerases enhances secretion of recombinant human transferrin in Schizosaccharomyces pombe., Applied Microbiology and Biotechnology, 86(4), 1135-1143 (2010), 2010.04.
105. Park J-H, Nishidate T, Kijima K, Ohashi T, Takegawa K, Hirata K, Nakamura Y, Katagiri T, Critical roles of Mucin 1 glycosylation by transactivated polypeptide N-acetylgalactosaminyltransferase 6 in mammary carcinogenesis., Cancer Research, 70(7), 2759-2769 (2010), 2010.04, 乳がん細胞においてO-結合型糖鎖の構造は変化することが知られているが、そのメカニズムについてはそれほどわかっていない。我々は多くの乳がん患者がムチン型O-結合型糖鎖に関与するN-アセチルガラクトサミニダーゼ6(GALNT6)の発現が上昇していることを見いだした。GALNT6の発現を抑制すると乳がん細胞の増殖は抑制される。またGALNT6がMUC1の糖鎖付加に関与してタンパク質を安定化させることを見いだした。この結果から、GALNT6の特異的な阻害剤は乳がんに対する新規薬剤になる可能性が示唆された。.
106. Suzuki S, Matsuzawa T, Nukigi Y, Takegawa K, Tanaka N, Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast., Microbiology, 156(3), 708-718 (2010), 2010.03.
107. Idiris A, Tohda H, Kumagai H, Takegawa K, Engineering of protein secretion in yeast: strategies and impact on protein production., Applied Microbiology and Biotechnology, 86(2), 403-417 (2010), 2010.03.
108. Ohashi T, Takegawa K, Structure of the N- and O-linked oligosaccharides that show complete loss of galactose residues from gms1och1 mutant of Schizosaccharomyces pombe., Applied Microbiology and Biotechnology, 86(1), 263-272 (2010), 2010.02.
109. Kimura Y, Kawasaki S, Yoshimoto H, Takegawa K, Glycine betaine biosynthesized from glycine provides an osmolyte for cell growth and spore germination during osmotic stress in Myxococcus xanthus., Journal of Bacteriology, 192(5), 1467-1470 (2010), 2010.02.
110. Idiris A, Tohda H, Kumagai H, Giga-Hama Y, Takegawa K, Enhanced protein secretion from the multiprotease-deletion fission yeast by modification of vacuolar protein sorting pathway., Applied Microbiology and Biotechnology, 85(3), 667-677 (2010), 2010.01.
111. Mukaiyama H, Kajiwara S, Hosomi A, Giga-Hama Y, Tanaka N, Namkamura T, Takegawa K, Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation., Microbiology, 155(12), 3816-3826 (2009), 2009.12.
112. Kimura Y, Kakemizu A, Matsubara Y, Takegawa K, Interaction between a Ser/Thr protein kinase, SpkA, and a cAMP-dependent protein kinase regulatory subunit homolog, CbpB, from Myxococcus xanthus., The Journal of General and Applied Microbiology, 55(6), 499-502 (2009), 2009.12.
113. Mukaiyama H, Tohda H, Giga-Hama Y, Takegawa K, Dextran sodium sulfate is effective enhancer for secretion of recombinant human transferrin in Schizosaccharomyces pombe., Applied Microbiology and Biotechnology, 85(1), 155-164 (2009), 2009.11.
114. Rothlisberger S, Jourdain I, Johnson C, Takegawa K, Hyams JS, The dynamin-related protein Vps1 regulates vacuole fisson, fusion and tubulation in the fission yeast, S. pombe., Fungal Genetics and Biology, 46(12), 927-935 (2009), 2009.11.
115. Goto M, Harada Y, Oka T, Matsumoto S, Takegawa K, Furukawa K, Protein O-mannosyltransferase B and C support hyphal development and differentiation in Aspergillus nidulans. , Eukaryotic Cell , 8(10), 1465-1474 (2009), 2009.10.
116. Kashiwazaki J, Iwaki T, Takegawa K, Shimoda C, Nakamura T, Two Schizosaccharomyces pombe Rab7 homologs, Ypt7 and Ypt71, play antagonistic roles in the regulation of vacuolar morphology., Traffic, 10(7), 912-924 (2009), 2009.07.
117. Nakakita S, Hasehira K, Hosokawa T, Tokuda M, Izumori K, Takegawa K, Hirabayashi J, Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses., Journal of Chromatography A, 1216(26), 5112-5115 (2009), 2009.07.
118. Takegawa K, Tohda H, Sasaki M, Idiris A, Ohashi T, Mukaiyama H, Giga-Hama Y, Kumagai H, Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system., Biotechnology and Applied Biochemistry, 53(4), 227-235 (2009), 2009.07.
119. Ohashi T, Ikeda Y, Tanaka N, Nakakita S, Natsuka S, Giga-Hama Y, Takegawa K, The och1 mutant of Schizosaccharomyces pombe produces galactosylated core structures of N-linked oligosaccharides., Bioscience, Biotechnology, and Biochemistry, 73(2), 407-414 (2009), 2009.02.
120. Ikeda Y, Ohashi T, Tanaka N, Takegawa K, Identification and characterization of a gene required for α1,2-mannose extension in the O-linked glycan synthesis pathway in Schizosaccharomyces pombe, FEMS Yeast Research, 9(1), 115-125 (2009), 2009.01.
121. Kimura Y, Okazaki N, Takegawa K, Enzymatic characteristics of two novel Myxococcus xanthus enzymes, PdeA and PdeB, displaying 3’,5’- and 2’,3’-cAMP phosphodiesterase, and phosphatase activities., FEBS Letters , 583(2), 443-448 (2009), 2009.01.
122. Kimura Y, Kakemizu A, Matsubara Y, Takegawa K, Enzymatic characteristics of a Ser/Thr protein kinase, SpkA, from Myxococcus xanthus., Journal of Bioscience and Bioengineering, 107(1), 10-15 (2009), 2009.01.
123. Chardwiriyapreecha, S., Shimazu, M., Morita, T., Sekito, T., Akiyama, K., Takegawa, K., and Kakinuma, Y., Identification of the fnx1+ and fnx2+ genes for vacuolar amino acid transporters in Schizosaccharomyces pombe., FEBS Letters , 582, 2225-2230 , 2008.07.
124. Li, B., Takegawa, K., Suzuki, T., Yamamoto, K., and Wang, L.-X., Synthesis and inhibitory activity of oligosaccharide thiazolines as a class of mechanism-based inhibitors for endo-β-N-acetylglucosaminidases., Bioorganic & Medicinal Chemistry, 16. 4670-4675 , 2008.04.
125. Iwaki, T., Iefuji, H., Hiraga, Y., Hosomi, A., Morita, T., Giga-Hama, Y., and Takegawa, K., Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe., Microbiology, 154, 830-841 , 2008.03.
126. Hosomi, A., Kawanishi, Y., Tanaka, N., and Takegawa, K., PXA domain-containing protein Pxa1 is required for normal vacuole function and morphology in Schizosaccharomyces pombe., Bioscience, Biotechnology, and Biochemistry , 72, 548-556 , 2008.02.
127. Takanashi, K., Shitan, N., Sugiyama, A., Kamimoto, Y., Hamamoto, M., Iwaki, T., Takegawa, K. and Yazaki, K., Galactinol synthase genes of Coptis japonica are involved in confers berberine tolerance in yeast, Bioscience, Biotechnology, and Biochemistry, 79, 398-405, 72, 398-405 , 2008.02.
128. Yasokawa, T., Ishimaru, I., Kuriyama, S., Masaki, T., Takegawa, K., and Tanaka, N., A double filtering method for measuring the translational velocity of fluorescently stained cells, Appl. Phys. Lett., 91, 131116, 91, 131116 , 2007.12.
129. Onishi, M., Iida, M., Koga, T., Yamada, S., Hirata, A., Iwaki, T., Takegawa, K., Fukui, Y., and Tachikawa, H., Fission yeast Sst4p, a conserved Vps27/Hrs homolog, functions downstream of PtdIns 3-kinase Pik3p to mediate proper spore formation. , Eukaryot. Cell , 6, 2343-2353 , 2007.12.
130. Iwaki, T., Onishi, M., Ikeuchi, M., Kita, A., Sugiura, R., Giga-Hama, Y., Fukui, Y., and Takegawa, K., Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe. , Microbiology , 153, 2753-2764 , 2007.11.
131. Fujita, K., Sato, R., Toma, K., Yamamoto, K., and Takegawa, K., Identification of the catalytic acid-base residue of Arthrobacter endo-β-N-acetylglucosaminidase by chemical rescue of inactive mutant., J. Biochem, 142, 301-306, 142, 301-306 , 2007.10.
132. Yasokawa, T., Ishimaru, I., Kondo, M., Kuriyama, S., Masaki, T., Takegawa, K., and Tanaka, N., A method for measuring the three-dimensional refractive-index distribution of single cells using proximal two-beam optical tweezers and a phase-shifting Mach-Zehnder interferometer., Opt. Rev., 14, 161-164, 14, 161-164 , 2007.08.
133. Yasokawa, T., Ishizaki, T., K. Ishizaki, K. Gesho, Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N., Technique for measuring the rotational velocity of a single cell., Appl. Phys. Lett., 90, 051103, 90, 051103 , 2007.07.
134. Miyatake, M., Kuno, T., Kita, A., Katsura, K., Takegawa, K., Nabata, T., and Sugiura, R., Valpronic acid affects trafficking and cell wall integrity in fission yeast. , Genetics, 175, 1695-1705, 2007.05.
135. Iwaki, T., Morita, T., Tanaka, N., Giga-Hama, Y., and Takegawa, K., Loss of a GPI-anchored membrane protein Aah3p causes a defect of vacuolar protein sorting in Schizosaccharomyces pombe., Biosci. Biotechnol. Biochem. , 71, 623-626, 71, 623-626 , 2007.04.
136. Yamamoto, K., Ishimaru, I., Fujii, Y., Kuriyama, S., Masaki, T., Takegawa, K., and Tanaka, N., Method for measuring the three dimensional distribution of a fluorescent dye in a cell membrane., Appl. Phys. Lett., 90, 021110, 90, 021110 , 2007.02.
137. Giga-Hama, Y., Tohda, H., Takegawa, K., and Kumagai, H., Minimum genome factory using fission yeast Schizosaccharomyces pombe, improvement of host genome for heterologous protein production., Biotechnol. Appl. Biochem. , 46, 147-155, 46, 147-155 , 2007.02.
138. Ma, Y., Sugiura, R., Saito, M., Koike, A., Sio, S.-O., Fujita, Y., Takegawa, K. and Kuno, T., New amino acid-auxotrophic markers for targeted gene integration and disruption in fission yeast. , Curr.Genet. , 52, 97-105 , 2007.02.
139. Kobayashi, H., Ishimaru, I., Yasokawa, T., Ishizaki, K., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N., Three dimentional phase-contrast Imaging for single floating cells., Appl. Phys. Lett. , 89, 241117, 89, 241117 , 2006.12.
140. Iwaki, T., Giga-Hama, Y., and Takegawa, K., A survey of all 11 ABC transporters in fission yeast: two novel ABC transporters are required for red pigment accumulation in a Schizosaccharomyces pombe adenine biosynthetic mutant. , Microbiology, 152, 2309-2321 , 2006.11.
141. Yoshida, M., Ishimaru, I., Ishizaki, K., Yasokawa, T., Kuriyama, S., Masaki, T., Takegawa, K., and Tanaka, N., Displacement measurement of the depth migration of transparent cells., Appl. Phys. Lett. , 89, 241102, 89, 241102 , 2006.11.
142. Inoue, Y., Ishimaru, I., Yasokawa, T., Ishizaki, K., Yoshida, M., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N., Variable phase-contrast fluorescence spectrometry for fluorescently stained cells., Appl. Phys. Lett. , 89, 121103 , 2006.10.
143. Yasokawa, T., Ishimaru, I., Nakagawa, T., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N., Attitudinal manipulation of an optically trapped bacillary probe by controlling the distance between focal points for local dosing in cells., Appl. Phys. Lett. , 89, 121103, 89, 131107 , 2006.10.
144. Fujita, Y., Tohda, H., Giga-Hama, Y., and Takegawa, K., Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe. , FEMS Yeast Res. , 6, 883-887 , 2006.07.
145. Mai, H.T., Nomura, M., Takegawa, K., Asamizu E., Sato S., Kato T., Tabata S., and Tajima, S., Identification of a Sed5-like SNARE gene LjSYP32-1 that contributes to nodule tissue formation of Lotus japonicus. , Plant Cell Physiol. , 47, 829-838 , 2006.07.
146. Iwaki, T., Hosomi, A., Tokudomi, S., Kusunoki, Y., Fujita, Y., Giga-Hama, Y., Tanaka, N., and Takegawa, K., Vacuolar protein sorting receptor in Schizosaccharomyces pombe. , Microbiology, 152, 1523-1532 , 2006.07.
147. Fujita, K., Sato, R., Toma, K., Yamamoto, K., and Takegawa, K., Identification of the catalytic acid-base residue of Arthrobacter endo-β-N-acetylglucosaminidase by chemical rescue of inactive mutant., J. Biochem. , 142, 301-306 , 2006.07.
148. Kobayashi, H., Ishimaru, I., Hyodo, R., Yasokawa, T., Ishizaki, T., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N., A pricise method for rotating single cells., Appl. Phys. Lett. , 88, 131103, 88, 131103 , 2006.07.
149. Ishizaki, K., Ishimaru, I., Yoshida, M., Inoue, Y., Yasokawa, T., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N., Translational velocity measurement for single floating cell based on optical Fourier transform theory., Appl. Phys. Lett. , 88, 101114, 88, 101114 , 2006.06.
150. Fujita, Y., Ukena, E., Iefuji, H., Giga-Hama, Y., and Takegawa, K., Homocysteine accumulation causes a defect in purine biosynthesis: further characterization of Schizosaccharomyces pombe methionine auxotrophs. , Microbiology , 152, 397-404 , 2006.02.
151. Hirashima K, Iwaki T, Takegawa K, Giga-Hama Y, Tohda H, A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast. , Nucleic Acids Res. , 34, e11 , 2006.02.
152. He, Y., Sugiura, R., Ma, Y., Kita, A., Deng, L., Takegawa, K., Matsuoka, K., Shuntoh, H., and Kuno, T., Genetic and functional interaction between Ryh1 and Ypt3: two Rab GTPases that function in S. pombe secretory pathway. , Genes Cells , 11, 207-221 , 2006.02.
153. Haneda, K., Takeuchi, M., Tagashira, M., Inazu, T., Toma, K., Isogai, Y., Hori, M., Kobayashi, K., Takeuchi, M., Takegawa, K., and Yamamoto, K., Chemo-enzymatic synthesis of eel calcitonin glycosylated at two sites with the same and different carbohydrate structures. , Carbohydr. Res, 341, 181-190 , 2006.01.
154. Iwaki T, Fujita Y, Tanaka N, Giga-Hama Y and Takegawa K, A mitochondrial ABC transporter Atm1p is required for protection against oxidative stress and vacuolar functions in Schizosaccharomyces pombe., Bioscience Biotechnology and Biochemistry, 10.1271/bbb.69.2109., 69, 2109-2116 (2005) , 2005.10.
155. Goldar MM, Nishie T, Ishikura Y, Fukuda T, Takegawa K and Kawamukai M, Functional conservation between fission yeast moc1/sds23 and its two orthologs, budding yeast SDS23 and SDS24, and phenotypic differences in their disruptants., Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.69.1422., 69, 1422-1426 (2005), 2005.08.
156. Tanaka N, Fujita Y, Suzuki S, Morishita M, Giga-Hama Y, Shimoda C and Takegawa K, Characterization of O-mannosyltransferase family in Schizosaccharomyces pombe., Biochemical and Biophysical Research Communiactions, 10.1016/j.bbrc.2005.03.033., 330, 813-820 (2005), 2005.05.
157. Kimura Y, Ohtani M and Takegawa K, An adenylyl cyclase, CyaB, acts as an osmosensor in Myxococcus xanthus., Journal of Bacteriology, 10.1128/JB.187.10.3593-3598.2005., 187, 3593-3598 (2005), 2005.04.
158. Fujita Y, Giga-Hama Y and Takegawa K, Development of a genetic transformation system using new selectable markers for fission yeast Schizosaccharomyces pombe., Yeast, doi: 10.1002/yea.1201., 22, 193-202 (2005), 2005.02.
159. Kashiwazaki J, Nakamura T, Iwaki T, Takegawa K and Shimoda C, A role for fission yeast Rab GTPase Ypt7p in sporulation., Cell Structure and Function, doi: 10.1247/csf.30.43., 30, 43-49 (2005), 2005.01.
160. Koga T, Onishi M, Nakamura Y, Hirata A, Nakamura T, Shimoda C, Iwaki T, Takegawa K and Fukui Y, Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe., Genes to Cells, 10.1111/j.1356-9597.2004.00744.x., 9, 561-574 (2004), 2004.09.
161. Iwaki T, Tanaka N, Takagi H, Giga-Hama Y and Takegawa K, Characterization of end4+, a gene required for endocytosis in Schizosaccharomyces pombe., Yeast, 10.1002/yea.1134., 21, 867-881 (2004), 2004.09.
162. Kita A, Sugiura R, He Y, Deng L, Lu Y, Sio SO, Takegawa K, Sakaue M, Shuntoh H and Kuno T, Loss of Apm1, the AP-1 clathrin-adaptor gamma1 subunit, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeast. , Molecular Biology of the Cell, 10.1091/mbc.e03-09-0659., 15, 2920-2931 (2004) , 2004.08.
163. Fujita K, Takami H, Yamamoto K and Takegawa K, Characterization of endo--N-acetylglucosaminidase from alkaliphilic Bacillus halodurans C-125., Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.68.1059., 68, 1059-1066 (2004), 2004.07.
164. Iwaki T, Goa T, Tanaka N and Takegawa K, Characterization of Schizosaccharomyces pombe mutants defective in vacuolar acidification and protein sorting., Molecular Genetics and Genomics , 10.1007/s00438-003-0971-7., 271, 197-207 (2004), 2004.06.
165. Morita T and Takegawa K, A simple and efficient procedure for transformation of Schizosaccharomyces pombe., Yeast, 10.1002/yea.1104., 21, 613-617 (2004), 2004.06.
166. Morita T, Takegawa K and Yagi T, Disruption of plr1+ gene encoding pyridoxal reductase of Schizosaccharomyces pombe. , Journal of Biochemistry, 10.1093/jb/mvh026., 135, 225-230 (2004), 2004.05.
167. Iwaki T and Takegawa K, A set of LoxP marker cassette for Cre-mediated multiple gene disruption in Schizosaccharomyces pombe. , Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.68.545., 68, 545-550 (2004) , 2004.04.
168. Fujita Y and Takegawa K, Characterization of two genes encoding putative cysteine synthase required for cysteine biosynthesis in Schizosaccharomyces pombe., Bioscience, Biotechnology and Biochemistry, 10.1271/bbb.68.306., 68, 306-311 (2004) , 2004.03.
169. Maeda T, Sugiura R, Deng L, He Y, Lu Y, Fujita Y, Takegawa K, Shuntoh H and Kuno T, Pmr1, a P-type ATPase, and Pdt1, an Nramp Homolog, cooperatively regulate in cell morphogenesis in fission yeast: The importance of Mn2+ homeostasis., Genes to Cells, 10.1111/j.1356-9597.2004.00699.x., 9, 71-82 (2004), 2004.02.
170. Takegawa K, Hosomi A, Iwaki T, Fujita Y, Morita T and Tanaka N, Identification of a SNARE protein required for vacuolar protein transport in Schizosaccharomyces pombe., Biochemical and Biophysical Research Communications, doi: 10.1016/j.bbrc.2003.09.179., 331, 77-82 (2003)., 2003.10.
171. Onishi M, Nakamura Y, Koga T, Takegawa K and Fukui Y, Isolation of suppressor mutants of phosphatidylinositol 3-phosphate 5-kinase deficient cells in Schizosaccharomyces pombe., Bioscience, Biotechnology and Biochemistry, doi: 10.1271/bbb.67.1772., 67, 1772-1779 (2003)., 2003.08.
172. Iwaki T, Osawa F, Onishi M, Koga T, Fujita Y, Hosomi A, Tanaka N, Fukui Y and Takegawa K, Characterization of vps33+, a gene required for vacuolar biogenesis and protein sorting in Schizosaccharomyces pombe., Yeast, doi: 10.1002/yea.1011., 20, 845-855 (2003)., 2003.06.
173. Takegawa K, Tokudomi S, Bhuiyan MSA, Tabuchi M, Fujita Y, Iwaki T, Utsumi S and Tanaka N, Heterologous expression and characterization of Schizosaccharomyces pombe vacuolar carboxypeptidase Y in Saccharomyces cerevisiae., Current Genetics, doi: 10.1007/s00294-002-0357-0., 42, 252-259 (2003)., 2003.04.
174. Onishi M, Koga T, Morita R, Nakamura Y, Nakamura T, Shimoda C, Takegawa K, Hirata A and Fukui Y, Role of phosphatidylinositol 3-phosphate in formation of forespore membrane in Schizosaccharomyces pombe., Yeast, doi: 10.1002/yea.953., 20, 193-206 (2003), 2003.02.
175. Nita-Lazar M, Chevolot L, Iwahara S, Takegawa K, Furmanek A and Lienart Y., High performance liquid chromatography and photodiode array detection of ferulic acid in Rubus protoplasts elicited by O-glycans from Fusarium sp. M7-1., Acta Biochimica Polonica, 49, 1019-1027 (2002)., 2002.10.
176. Cheng H, Sugiura R, Wu W, Fujita M, Lu Y, Sio SO, Kawai R, Takegawa K, Shuntoh H and Kuno T., Role of the Rab GTP-binding protein Ypt3 in the fission yeast exocytic pathway, and its connection to calcineurin function., Molecular Biology of the Cell, doi: 10.1091/mbc.01-09-0463., 13, 2963-2976 (2002)., 2002.08.
177. Kimura Y, Mishima Y, Nakano H and Takegawa K., An adenylyl cyclase, CyaA, of Myxococcus xanthus functions in signal transduction during osmotic stress., Journal of Bacteriology, doi: 10.1128/JB.184.13.3578-3585.2002., 184, 3578-3585 (2002). , 2002.04.
178. Fujita K, Miyamura T, Sano M, Kato I and Takegawa K., Transfer of high-mannose type oligosaccharides to disaccharides by endo--N-acetylglucosaminidase from Arthrobacter protophormiae., Journal of Bioscience and Bioengineering, 93, 614-617 (2002)., 2002.02.
179. Fujita M, Shoda S, Haneda K, Inazu T, Takegawa K and Yamamoto K, A novel disaccharide substrate having 1,2-oxazoline moiety for detection of transglycosylating activity of endoglycosidases., Biochimica et Biophysica Acta, doi: 10.1016/s0304-4165(01)00164-7., 1528, 9-14 (2001)., 2001.08.
180. Tanaka N, Konomi M, Osumi M and Takegawa K, Characterization of a Schizosaccharomyces pombe mutant deficient in UDP-galactose transport activity., Yeast, doi: 10.1002/yea.740., 18, 903-914 (2001)., 2001.06.
181. Fujita K, Nakatake R, Yamabe K, Watanabe A, Asada Y and Takegawa K, Identification of amino acid residues essential for the substrate specificity of Flavobacterium sp. endo-beta-N-acetylglucosaminidase., Bioscience, Biotechnology, and Biochemistry, doi: 10.1271/bbb.65.1542., 65, 1542-1548 (2001)., 2001.06.
182. Bhuiyan MSA, Ito Y, Tanaka N, Sadik G, Fujita K, Nakamura A, Biswas MH, Fukui H and Takegawa K, Nystatin sensitive and vacuolar protein sorting defective (vps) mutants of Saccharomyces cerevisiae: Their isolation and characterization., Pakistan Journal of Biological Sciences, 4, 259-262 (2001)., 2001.05.
183. Tanaka N and Takegawa K, Functional characterization of Gms1p/UDP-galactose transporter in Schizosaccharomyces pombe., Yeast, doi: 10.1002/yea.725. , 18, 745-757 (2001)., 2001.05.
184. Fujita K and Takegawa K, Tryptophan-216 is essential for the transglycosylation activity of endo--N-acetylglucosaminidase A., Biochemical and Biophysical Research Communications, doi: 10.1006/bbrc.2001.4836, 283, 680-686 (2001)., 2001.04.
185. Uo T, Yoshimura T, Tanaka N, Takegawa K and Esaki N, Functional characterization of alanine racemase from Schizosaccharomyces pombe: A eucaryotic counterpart to bacterial alanine racemase.
, Journal of Bacteriology, doi: 10.1128/JB.183.7.2226-2233.2001, 183, 2226-2233 (2001), 2001.03.
186. Fujita K and Takegawa K, Chemoenzymatic synthesis of neoglycoproteins using transglycosylation with endo-beta-N-acetylglucosaminidase A. , Biochemical and Biophysical Research Communications, doi: 10.1006/bbrc.2001.4631, 282, 678-682 (2001)., 2001.03.
187. Kimura Y, Nakano H, Terasaka H and Takegawa K, Myxococcus xanthus mokA encodes a histidine kinase-response regulator hybrid sensor required for development and osmotic torelance., Journal of Bacteriology, doi: 10.1128/JB.183.4.1140-1146.2001, 183, 1140-1146 (2001), 2001.02.
188. Fujita K, Asada Y, Yamamoto K and Takegawa K, A plate assay for endo-beta-N-acetylglucosaminidase activity using a chromogenic substrate synthesized by transglycosylation with Arthrobacter endo-beta-N-acetylglucosaminidase., Journal of Bioscience and Bioengineering, 90, 462-464 (2000)., 2000.08.
189. Fujita K, Tanaka N, Sano M, Kato I, Asada Y and Takegawa K, Synthesis of neoglycoenzymes with homogeneous N-linked oligosaccharides using immobilized endo-beta-N-acetylglucosaminidase A. , Biochemical and Biophysical Research Communications, doi: 10.1006/bbrc.1999.1963., 267, 134-138 (2000)., 2000.06.
190. Nita-Lazar M, Iwahara S, Takegawa K and Lienart Y, The active oxygen responce of raspberry protoplasts to O-glycans of Fusarium sp. M7-1., Journal of Plant Physiology, 156, 306-311 (2000)., 2000.04.
191. Tabuchi M, Yoshida T, Takegawa K and Kishi F, Functional analysis of the human NRAMP family expressed in fission yeast., Biochemical Journal, 344, 211-219 (1999)., 1999.09.
192. Segawa H, Ishida N, Takegawa K and Kawakita M, Schizosaccharomyces pombe UDP-galactose transporter: identification of its functional form through cDNA cloning and expression in mammalian cells. , FEBS Letters, doi: 10.1016/s0014-5793(99)00596-7., 451, 295-298 (1999)., 1999.07.
193. Tanaka N, Awai A, Bhuiyan MSA, Fujita K, Fukui H and Takegawa K, Cell-surface galactosylation is essential for non-sexual flocculation in Schizosaccharomyces pombe., Journal of Bacteriology, doi: 10.1128/JB.181.4.1356-1359.1999., 181, 1356-1359 (1999)., 1999.05.
194. Bhuiyan MSA, Ito Y, Nakamura A, Tanaka N, Fujita K, Fukui H and Takegawa K, Nystatin effects on vacuolar function in Saccharomyces cerevisiae. , Bioscience, Biotechnology, and Biochemistry, doi: 10.1271/bbb.63.1075., 63, 1075-1082 (1999)., 1999.05.
195. Yamamoto K, Haneda K, Iguchi R, Inazu T, Mizuno M, Takegawa K, Kondo A and Kato I, Chemo-enzymatic synthesis of a calcitonin derivative containing a high-mannose type oligosaccharide by endo--N-acetylglucosaminidase from Arthrobacter protophormiae., Journal of Bioscience and Bioengineering, 87, 175-179 (1999)., 1999.03.
196. Aiba H, Kawaura R, Yamamoto E, Yamada H, Takegawa K and Mizuno T, Isolation and characterization of high-osmolarity-sensitive mutants in fission yeast. , Journal of Bacteriology, doi: 10.1128/JB.180.19.5038-5043.1998., 180, 5038-5043 (1998). , 1998.07.
197. Tanaka N, Ohuchi N, Osaka Y, Mukai Y, Ohtani Y, Tabuchi M, Bhuiyan MSA, Fukui H, Harashima S and Takegawa K, Isolation and characterization of an invertase and its repressor genes from Schizosaccharomyces pombe., Biochemical and Biophysical Research Communications, doi: 10.1006/bbrc.1998.8406., 245, 246-253 (1998)., 1998.05.
198. Deras IL, Takegawa K, Kondo A, Kato I and Lee YC, Synthesis of a high-mannose-type oligosaccharide glycopeptide analog containing a glucose-asparagine linkage., Bioorganic&Medicinal Chemistry Letters, doi: 10.1016/s0960-894x(98)00306-0., 8, 1763-1766 (1998)., 1998.05.
199. Takegawa K, Fujita K, Fan J-Q, Tabuchi M, Tanaka N, Kondo A, Iwamoto H, Kato I, Lee YC and Iwahara S, Enzymatic synthesis of a neoglycoconjugate by transglycosylation with Arthrobacter endo-beta-N-acetylglucosaminidase: a substrate for colorimetric detection of endo-beta-N-acetylglucosaminidase activity., Analytical Biochemistry, doi: 10.1006/abio.1997.2543., 257, 218-223 (1998)., 1998.03.
200. Takegawa K, Satoh K, Nahrowi R, Jikibara T and Iwahara S, Production and characterization of extracellular uronic acid containing-glycoproteins from Fusarium oxysporum., Journal of Fermentation and Bioengineering, 83, 184-187 (1997)., 1997.05.
201. Tabuchi M, Tanaka N, Iwahara S and Takegawa K, The Schizosaccharomyces pombe gms1+ gene encodes an UDP-galactose transporter homologue required for protein galactosylation., Biochemical and Biophysical Research Communications, doi: 10.1006/bbrc.1997.6239., 232, 121-125 (1997)., 1997.05.
202. Tabuchi M, Iwaihara O, Ohtani Y, Ohuchi N, Sakurai J, Morita T, Iwahara S and Takegawa K, Vacuolar protein sorting in fission yeast: cloning, biosynthesis, transport, and processing of carboxypeptidase Y from Schizosaccharomyces pombe. , Journal of Bacteriology, doi: 10.1128/jb.179.13.4179-4189.1997., 179, 4179-4189 (1997)., 1997.05.
203. Takegawa K, Yamabe K, Fujita K, Tabuchi M, Mita M, Watanabe A, Asada Y, Sano M, Kondo A, Kato I and Iwahara S, Cloning, sequencing, and expression of Arthrobacter protophormiae endo-beta-N-acetylglucosaminidase in Escherichia coli., Archives of Biochemistry and Biophysics, doi: 10.1006/abbi.1996.9803., 338, 22-28 (1997)., 1997.03.
204. Takegawa K, Tanaka N, Tabuchi M and Iwahara S, Isolation and characterization of a glycosylation mutant from Schizosaccharomyces pombe., Bioscience, Biotechnology, and Biochemistry, doi: 10.1271/bbb.60.1156., 60, 1156-1159 (1996)., 1996.07.
205. Fan J-Q, Huynh LH, Reinhold BB, Reinhold VN, Takegawa K, Iwahara S, Kondo A, Kato I and Lee YC, Transfer of Man9GlcNAc to L-fucose by endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae., Glycoconjugate Journal, doi: 10.1007/BF00731453., 13, 643-652 (1996)., 1996.05.
206. Iwahara S, Suemori N and Takegawa K, Isolation and identification of a choline-linked mannobiose in the glycoproteins of Fusarium sp. M7-1., Bioscience, Biotechnology, and Biochemistry, doi: 10.1271/bbb.60.349., 60, 349-350 (1996)., 1996.03.
207. Takegawa K, DeWald DB and Emr SD, Schizosaccharomyces pombe Vps34p, a phosphatidylinositol-specific PI 3-kinase essential for normal cell growth and vacuole morphology., Journal of Cell Science, doi: 10.1242/jcs.108.12.3745., 108, 3745-3756 (1995)., 1995.10.
208. Zhou K, Takegawa K, Emr SD and Firtel RA, A phosphatidylinositol (PI) kinase gene family in Dictyostelium discoideum : biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development., Molecular and Cellular Biology, doi: 10.1128/MCB.15.10.5645., 15, 5645-5656 (1995). , 1995.08.
209. Fan J-Q, Takegawa K, Iwahara S, Kondo A, Kato I, Abeygunawardana C and Lee YC, Enhanced transglycosylation activity of Arthrobacter protophormiae endo-beta-N-acetylglucosaminidase in media containing organic solvents., The Journal of Biological Chemistry, doi: 10.1074/jbc.270.30.17723., 270, 17730-17735 (1995)., 1995.07.
210. Fan J-Q, Quesenberry MS, Takegawa K, Iwahara S, Kondo A, Kato I and Lee YC, Synthesis of neoglycoconjugates by transglycosylation with Arthrobacter protophormiae endo-beta-N-acetylglucosaminidase : Demonstration of a macro-cluster effect for mannose-binding proteins., The Journal of Biological Chemistry, doi: 10.1074/jbc.270.30.17730., 270, 17730-17735 (1995)., 1995.07.
211. Ramli N, Fujinaga M, Tabuchi M, Takegawa K and Iwahara S, Isolation and characterization of novel endo-beta-galactofuranosidase from Bacillus sp., Bioscience, Biotechnology, and Biochemistry, doi: 10.1271/bbb.59.1856., 59, 1856-1860 (1995)., 1995.06.
212. Iwahara S, Ramli N and Takegawa K, Degradation of beta1-6galactofuranoside linkages in the polysaccharide of Fusarium sp. M7-1 by endo-beta-galactofuranosidase from Bacillus sp., Bioscience, Biotechnology, and Biochemistry, doi: 10.1271/bbb.60.957., 60, 957-961 (1996)., 1995.05.
213. Stack JH, DeWald DB, Takegawa K and Emr SD, Vesicle-mediated protein transport: Regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast., Journal of Cell Biology, doi: 10.1083/jcb.129.2.321., 129, 321-334 (1995)., 1995.05.
214. Iwahara S, Suemori N, Ramli N, and Takegawa K, Isolation and identification of novel acidic oligosaccharides derived from glycoproteins of Fusarium sp. M7-1., Bioscience, Biotechnology, and Biochemistry, doi: 10.1271/bbb.59.1082., 59, 1082-1085 (1995)., 1995.03.
215. Takegawa K, Tabuchi M, Yamaguchi S, Kondo A, Kato I and Iwahara S, Synthesis of neoglycoproteins using oligosaccharide-transfer activity with endo-beta-N-acetylglucosaminidase., The Journal of Biological Chemistry, doi: 10.1074/jbc.270.7.3094., 270, 3094-3099 (1995)., 1995.02.
216. Ramli N, Shinohara H, Takegawa K and Iwahara S, Preparation of beta (1-6)-linked galactofuranoside oligomers from the acidic polysaccharide of Fusarium sp. M7-1., Journal of Fermentation and Bioengineering, 78, 341-345 (1994). , 1994.07.
217. Ramli N, Takegawa K and Iwahara S, Degradation of unsaturated polysaccharide derived from acidic polysaccharide of Fusarium sp. M7-1 by a bacterium isolated from soil., Journal of Fermentation and Bioengineering, 77, 572-574 (1994)., 1994.05.
218. Welters P, Takegawa K, Emr S and Chrispeels MJ, AtVPS34, a phosphatidylinositol 3-kinase of Arabidopsis thaliana is an essential protein with homology to a calcium-dependent lipid binding domain., The Proceedings of the National Academy of Sciences, doi: 10.1073/pnas.91.24.11398., 91, 11398-11402 (1994)., 1994.05.
219. Iwahara S, Takegawa K, Kawaguchi K and Okamoto G, Isolation and identification of ethyl--N-acetylglucosaminide from yeast extract., Bioscience, Biotechnology, and Biochemistry, 57, 1779-1780 (1993)., 1993.07.
220. Iwahara S, Takegawa K, Kawaguchi K and Okamoto G, The presence of trehalose-containing oligosaccharides in yeast extract., Bioscience, Biotechnology, and Biochemistry, doi: 10.1271/bbb.57.1220., 57, 1220-1221 (1993). , 1993.05.
221. Schu PV, Takegawa K, Fry MJ, Stack JH, Waterfield MD and Emr SD, Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting., Science, doi: 10.1126/science.8385367., 260, 88-91 (1993)., 1993.04.
222. Iwahara S, Maeyama T, Mishima T, Jikibara T, Takegawa K and Iwamoto H, Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: IV. Isolation and identification of four novel oligosaccharide units derived from the acidic polysaccharide chain., Journal of Biochemistry, 10.1093/oxfordjournals.jbchem.a123905., 112, 355-359 (1992)., 1992.07.
223. Jikibara T, Tada K, Takegawa K and Iwahara S, Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: II. The primary structures of the low molecular weight carbohydrate chains of the glycoproteins., Journal of Biochemistry, 10.1093/oxfordjournals.jbchem.a123742., 111, 230-235 (1992), 1992.03.
224. Jikibara T, Takegawa K and Iwahara S, Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: III. The primary structures of the acidic polysaccharides of the glycoproteins., Journal of Biochemistry, 10.1093/oxfordjournals.jbchem.a123743., 111, 236-243 (1992)., 1992.03.
225. Jikibara T, Takegawa K and Iwahara S, Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: I. Isolation and some properties of the glycoproteins. , Journal of Biochemistry, 10.1093/oxfordjournals.jbchem.a123741, 111, 225-229 (1992), 1992.01.
226. Takegawa K, Yamaguchi S, Kondo A, Kato I and Iwahara S, Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae., Biochemistry International, 25, 829-835 (1991)., 1991.07.
227. Takegawa K, Yamaguchi S, Kondo A, Iwamoto H, Nakoshi M, Kato I and Iwahara S, Transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae., Biochemistry International, 25, 181-190 (1991)., 1991.06.
228. Takegawa K, Yoshikawa M, Mishima T, Nakoshi M and Iwahara S, Determination of glycosylation sites using a protein sequencer and deglycosylation of native yeast invertase by endo-beta-N-acetylglucosaminidase., Biochemistry International, 25, 585-592 (1991)., 1991.06.
229. Takegawa K, Kondo A, Iwamoto H, Fujiwara K, Hosokawa Y, Kato I, Hiromi K and Iwahara S, Novel oligomannose-type sugar chains derived from glucose oxidase of Aspergillus niger., Biochemistry International, 25, 181-190 (1991)., 1991.06.
230. Takegawa K, Mikami B, Iwahara S, Morita Y, Yamamoto K and Tochikura T, Complete amino acid sequence of endo-beta-N-acetylglucosaminidase from Flavobacterium sp., Europian Journal of Biochemistry, 10.1111/j.1432-1033.1991.tb16359.x., 202, 175-180 (1991), 1991.05.
231. Takegawa K, Naitoh T and Iwahara S, Isolation of two endo-beta-N-acetylglucosaminidases with different specificities from Pseudomonas sp., Biochemistry International, 24, 793-799 (1991). , 1991.05.
232. Takegawa K, Iwahara K and Iwahara S, Purification and properties of chondroitinase produced by a bacterium isolated from soil., Journal of Fermentation and Bioengineering, 72, 128-131 (1991)., 1991.04.
233. Takegawa K, Yamaguchi S, Miki S, Jikibara T and Iwahara S, Purification and characterization of a novel lyase from Cellulomonas sp. that degrades Fusarium and Gibberella acidic polysaccharides., Agricultural and Biological Chemistry, 55, 1969-1975 (1991)., 1991.03.
234. Takegawa K, Nakoshi M, Yamamoto K, Tochikura T and Iwahara S, Activation of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae by various sugars., Journal of Fermentation and Bioengineering, 71, 278-280 (1991)., 1991.02.
235. Takegawa K, Fujiwara K, Iwahara S, Yamamoto K and Tochikura T, Primary structure of an O-linked sugar chain derived from glucose oxidase of Aspergillus niger., Agricultural and Biological Chemistry, 55, 883-884 (1991)., 1991.01.
236. Takegawa K, Miki S and Iwahara S, Effect of deglycosylation of polymannose chains on the properties of yeast external invertase., Journal of Fermentation and Bioengineering, 70, 131-133 (1990)., 1990.08.
237. Iwahara S, Jikibara T and Takegawa K, Identification of O-beta-D-glucopyranosyluronate-(1-2)-D-galactose isolated from the acidic polysaccharide of Fusarium sp. M7-1., Agricultural and Biological Chemistry, 54, 1559-1561 (1990)., 1990.06.
238. Takegawa K, Miki S, Jikibara T and Iwahara S, Purification and properties of low-molecular-weight alpha-mannosidase from Cellulomonas sp., Journal of Fermentation and Bioengineering, 69, 129-131 (1990)., 1990.03.
239. Iwahara S, Jikibara T, Miki S and Takegawa K, Beta-eliminative cleavage of the acidic polysaccharide of Fusarium sp. M7-1 by an enzyme preparation of Cellulomonas sp., Agricultural and Biological Chemistry, 54, 419-425 (1990)., 1990.01.
240. Takegawa K, Nakoshi M, Iwahara S, Yamamoto K and Tochikura T, Induction and purification of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae grown in ovalbumin., Applied and Environmental Microbiology, 10.1128/aem.55.12.3107-3112.1989., 55, 3107-3112 (1989)., 1989.10.
241. Takegawa K, Fujiwara K, Iwahara S, Yamamoto K and Tochikura T, Effect of deglycosylation of N-linked sugar chains on glucose oxidase from Aspergillus niger., Biochemistry and Cell Biology, 10.1139/o89-072., 67, 460-464 (1989)., 1989.07.
242. Takegawa K, Miki S, Osaka F, Jikibara T and Iwahara S, Action of beta-mannosidases on O--D-mannopyranosyl-(1-2)-D-mannose., Agricultural and Biological Chemistry, 53, 1179-1180 (1989)., 1989.05.
243. Takegawa K, Miki S, Jikibara T and Iwahara S, Purification and characterization of exo-alpha-D-mannosidase from a Cellulomonas sp., Biochimica et Biophysica Acta, 991, 431-437 (1989)., 1989.03.
244. Takegawa K, Kawasaki N, Iwahara S, Yamamoto K, Tochikura T, Mikami B and Morita Y, Primary structure of an N-linked sugar chain derived from glucoamylase of Rhizopus niveus. , Biochimica et Biophysica Acta, 10.1016/s0304-4165(89)80018-2., 990, 98-100 (1989), 1989.01.
245. Takegawa K, Inami M, Yamamoto K, Kumagai H, Tochikura T, Mikami B and Morita Y, Deglycosylated glucoamylase from Rhizopus niveus is precipitated by Flavobacterium sp. endo-beta-N-acetylglucosaminidase., Agricultural and Biological Chemistry, 52, 2941-2942 (1988)., 1988.09.
246. Takegawa K, Inami M, Yamamoto K, Kumagai H, Tochikura T, Mikami B and Morita Y, Elucidation of the role of sugar chains in glucoamylase using endo-beta-N-acetylglucosaminidase from Flavobacterium sp., Biochimica et Biophysica Acta, 955, 187-193 (1988)., 1988.07.
247. Kadowaki S, Takegawa K, Yamamoto K, Kumagai H and Tochikura T, Effect of yeast extract on endo--N-acetylglucosaminidase production by a Flavobacterium sp., Agricultural and Biological Chemistry, 52, 2105-2106 (1988)., 1988.03.
248. Yamamoto K, Takegawa K, Kumagai H and Tochikura T, Effect of protease digestion on the activity of sugar-depleted enzymes prepared with endo--N-acetylglucosaminidase from Flavobacterium sp., Agricultural and Biological Chemistry, 51, 1481-1487 (1987)., 1987.01.
249. Yamamoto K, Takegawa K, Kumagai H and Tochikura T, Elucidation of the role of sugar chains in glycosylated-enzymes using endo--N -acetylglucosaminidase from Flavobacterium sp., Agricultural and Biological Chemistry , 50, 2167-2169 (1986)., 1986.05.
250. Yamamoto K, Takegawa K, Fan J, Kumagai H and Tochikura T, The release of oligosaccharides from glycoproteins by endo--N-acetylglucosaminidase of Flavobacterium sp., Journal of Fermentation Technology, 64, 397-403 (1986). , 1986.03.
251. Yamamoto K, Kadowaki S, Takegawa K, Kumagai H and Tochikura T, Purification and characterization of endo--N-acetylglucosaminidase from a Flavobacterium sp., Agricultural and Biological Chemistry, 50, 421-429 (1986)., 1986.01.