Kyushu University Academic Staff Educational and Research Activities Database
List of Books
Atsushi SUZUKI Last modified date:2021.06.29

Professor / Department of Molecular and Cellular Biology / Medical Institute of Bioregulation

1. Shizuka Miura, Atsushi Suzuki, Intestinal Stem Cells: Direct Lineage Reprogramming of Mouse Fibroblasts to Acquire the Identity of Fetal Intestine-Derived Progenitor Cells, Methods in Molecular Biology, Springer Protocols, 10.1007/978-1-0716-0747-3_14, 2020.07, Intestinal organoids are useful models for studying the characteristics of intestinal diseases and their treatment. However, a major limiting factor in their usability is the need for donor tissue fragments or pluripotent stem cells to generate the organoids. Here, we describe an approach to generate intestinal organoids from fibroblasts, a new source. We used direct reprogramming technology to generate cells with the properties of fetal intestine-derived progenitor cells (FIPCs) from mouse embryonic fibroblasts (MEFs). These induced FIPCs (iFIPCs) can give rise to cells resembling intestinal stem cells (ISCs), henceforth referred to as induced ISCs (iISCs). These iFIPCs and iISCs form spherical and budding organoids, respectively, similar to FIPCs and ISCs. These induced intestinal organoids could be used for studies on intestinal diseases and regenerative therapy..