| 安尾 しのぶ（やすお しのぶ） | データ更新日：2023.12.06 |
教授 ／
農学研究院
資源生物科学部門
動物・海洋生物科学講座
| 1. | Emi Taniguchi, Ayumi Hattori, Kaito Kurogi, Yukihiro Hishida, Fumiko Watanabe, Mitsuhiro Furuse, Shinobu Yasuo, Temporal patterns of increased growth hormone secretion in mice after oral administration of L- ornithine: possible involvement of ghrelin receptors, The Journal of Veterinary Medical Science, 10.1292/jvms.22-0125., 84, 1283-1287, 2022.09. |
| 2. | Michihiro Ohashi, Sang-Il Lee, Taisuke Eto, Nobuo Uotsu, Chie Tarumizu, Sayuri Matsuoka, Shinobu Yasuo, Shigekazu Higuchi, Intake of l-serine before bedtime prevents the delay of the circadian phase in real life, Journal of Physiological Anthropology, 10.1186/s40101-022-00306-z, 41, 1, 2022.08, Abstract Background It has been shown in laboratory experiments using human subjects that ingestion of the non-essential amino acid l-serine before bedtime enhances the advance of circadian phase induced by light exposure the next morning. In the present study, we tested the effect of ingestion of l-serine before bedtime on circadian phase in real life and whether its effect depends on the initial circadian phase. Methods The subjects were 33 healthy male and female university students and they were divided into an l-serine group (n = 16) and a placebo group (n = 17). This study was conducted in a double-blind manner in autumn and winter. After a baseline period for 1 week, the subjects took 3.0 g of l-serine or a placebo 30 min before bedtime for 2 weeks. Saliva was collected twice a week at home every hour under a dim light condition from 20:00 to 1 h after habitual bedtime. Dim light melatonin onset (DLMO) was used as an index of phase of the circadian rhythm. Results DLMO after intervention was significantly delayed compared to the baseline DLMO in the placebo group (p = 0.02) but not in the l-serine group. There was a significant difference in the amount of changes in DLMO between the two groups (p = 0.04). There were no significant changes in sleeping habits after intervention in the two groups. There were significant positive correlations between advance of DLMO and DLMO before intervention in the l-serine group (r = 0.53, p < 0.05) and the placebo group (r = 0.69, p < 0.01). There was no significant difference in the slopes of regression lines between the two groups (p = 0.71), but the intercept in the l-serine group was significantly higher than that in the placebo group (p < 0.01). The levels of light exposure were not significantly different between the two groups. Conclusions Our findings suggest that intake of l-serine before bedtime for multiple days might attenuate the circadian phase delay in the real world and that this effect does not depend on the initial circadian phase. Trial registration This study is registered with University Hospital Medical Information Network in Japan (UMIN000024435. Registered on October 17, 2016).. |
| 3. | Dan Yang, Hideaki Oike, Mitsuhiro Furuse, Shinobu Yasuo, Spermidine resets circadian clock phase in NIH3T3 cells., Biomedical research (Tokyo, Japan), 10.2220/biomedres.42.221, 42, 5, 221-227, 2021.09, Irregular light-dark cycles desynchronize the circadian clock via hormonal and neuronal pathways and increase the risk of various diseases. This study demonstrated that a single pulse of spermidine-a polyamine-strongly induced circadian phase advances in the presence or absence of dexamethasone (a synthetic glucocorticoid) in NIH3T3 cells transfected with the Bmal1 promotor-driven luciferase reporter gene. The spermidine-induced phase advances were 2-3 fold greater than were the dexamethasone-induced shifts. The phase resetting effect of spermidine occurred in a dose- and time-dependent manner and was not blocked by RU486, an antagonist of glucocorticoid receptors. Spermidine treatment modulated the expression of clock genes within 60 min, which was sooner than changes in the expression of autophagy-related genes. These findings suggested that spermidine is a potent modulator of the circadian phase, acting through glucocorticoid receptor-independent pathways, and may be useful for treating diseases related to circadian desynchrony.. |
| 4. | Dan Yang, Hideaki Oike, Mitsuhiro Furuse, Shinobu Yasuo, Effect of regular and irregular stimulation cycles of dexamethasone on circadian clock in NIH3T3 cells., Chronobiology international, 10.1080/07420528.2021.1977654, 1-9, 2022.01, Animal studies have shown that irregular light-dark cycles cause circadian desynchronization, while few studies have addressed the effect of regular/irregular stimulation cycles of signaling hormones on the cellular clock in vitro. Here, we examined how cellular clocks respond to regular and irregular stimulation cycles of dexamethasone, using NIH3T3 cells transfected with the Bmal1 promoter-driven luciferase (Bmal1-Luc) reporter gene. Cyclic stimulation with dexamethasone at different time intervals (18-28 h, 3 times regularly) revealed that Bmal1-Luc bioluminescence rhythms can be entrained to 22 and 24 h cycles during the stimulation period, but not to other cycles. The rhythm entrained for 24 h cycles persisted for at least one day after the last stimulation. Irregular dexamethasone treatment (16, 24, and 16 h, sequentially; short-term jet lag protocol) resulted in an overall upregulation and phase shifts of the temporal expression of several clock genes and cell cycle genes, including c-Myc and p53. Regular dexamethasone stimulation three times with 24 h cycles also caused upregulation of Per1 and Per2 expression, but not c-Myc and p53 expression. In conclusion, our study identified the entrainable range of the circadian clock in NIH3T3 cells to the dexamethasone stimulation cycle and demonstrated that irregular dexamethasone treatment could disturb the expression of cell cycle genes.. |
| 5. | Emi Taniguchi, Ayako Tashiro, Ayumi Hattori, Mitsuhiro Furuse, Shinobu Yasuo , Photoperiodic changes in hippocampal neurogenesis and plasma metabolomic profiles in relation to depression-like behavior in mice, Behavioural Brain Research, 402:113100, 402, 113100, 2021.04. |
| 6. | Nozomu Takaki, Tatsuhiro Uchiwa, Mitsuhiro Furuse, Shinobu Yasuo, Effect of postnatal photoperiod on DNA methylation dynamics in the mouse brain, Brain Research, 10.1016/j.brainres.2020.146725, 1733, 2020.04, [URL], Season of birth influences the onset of psychiatric diseases in mammals. Recent studies using rodent models have revealed that photoperiod during early life stages has a strong impact on affective and cognitive behaviors, neuronal activity, and hippocampal neurogenesis/astrogenesis in later life. The present study examined the effect of postnatal photoperiod on global DNA methylation and hydroxymethylation dynamics in the mouse brain. Male mice born under short-day (SD) conditions were divided into SD and long-day (LD) groups on the day of birth. Temporal expression of DNA methyltransferases (DNMT1/3a) with 5-methylcytosine (5-mC) levels, as well as protein levels of ten-eleven translocation (TET) 2 with 5-hydroxymethylcytosine (5-hmC) levels, were analyzed from postnatal day 4 (P4) to P21. Levels of 5-hmC in all hippocampal areas were higher in the LD group than in the SD group at P21, with a positive correlation between 5-hmC levels and TET2 levels throughout the experimental period. Inconsistent results were observed between DNMT1/3a mRNA levels and 5-mC levels. On the other hand, in the OB, mRNA levels of DNMT1 and DNMT3a were slightly lower in the LD group similar to 5-mC levels, but TET2 and 5-hmC levels were not influenced by the photoperiod. In conclusion, postnatal exposure of mice to LD conditions induces an increase in TET2-dependent DNA hydroxymethylation in the hippocampus, which might be involved in the long-term effects of postnatal photoperiod on neurogenesis and affective/cognitive behaviors.. |
| 7. | Takuma Nishigawa, Satsuki Nagamachi, Vishwajit S. Chowdhury, Shinobu Yasuo, Mitsuhiro Furuse, Taurine and β-alanine intraperitoneal injection in lactating mice modifies the growth and behavior of offspring, Biochemical and Biophysical Research Communications, 10.1016/j.bbrc.2017.12.063, 2017.01, [URL], Taurine, one of the sulfur-containing amino acids, has several functions in vivo. It has been reported that taurine acts on γ-aminobutyric acid receptors as an agonist and to promote inhibitory neurotransmission. Milk, especially colostrum, contains taurine and it is known that milk taurine is essential for the normal development of offspring. β-Alanine is transported via a taurine transporter and a protein-assisted amino acid transporter, the same ones that transport taurine. The present study aimed to investigate whether the growth and behavior of offspring could be altered by modification of the taurine concentration in milk. Pregnant ICR mice were separated into 3 groups: 1) a control group, 2) a taurine group, and 3) a β-alanine group. During the lactation periods, dams were administered, respectively, with 0.9% saline (10 ml/kg, i.p.), taurine dissolved in 0.9% saline (43 mg/10 ml/kg, i.p.), or β-alanine dissolved in 0.9% saline (31 mg/10 ml/kg, i.p.). Interestingly, the taurine concentration in milk was significantly decreased by the administration of β-alanine, but not altered by the taurine treatment. The body weight of offspring was significantly lower in the β-alanine group. β-Alanine treatment caused a significant decline in taurine concentration in the brains of offspring, and it was negatively correlated with total distance traveled in the open field test at postnatal day 15. Thus, decreased taurine concentration in the brain induced hyperactivity in offspring. These results suggested that milk taurine may have important role of regulating the growth and behavior of offspring.. |
| 8. | Takahiro Kawase, Mao Nagasawa, Hiromi Ikeda, Shinobu Yasuo, Yasuhiro Koga, Mitsuhiro Furuse, Gut microbiota of mice putatively modifies amino acid metabolism in the host brain, British Journal of Nutrition, 10.1017/S0007114517000678, 117, 6, 775-783, 2017.03, [URL], Recently, it has been found that the gut microbiota influences functions of the host brain by affecting monoamine metabolism. The present study focused on the relationship between the gut microbiota and the brain amino acids. Specific pathogen-free (SPF) and germ-free (GF) mice were used as experimental models. Plasma and brain regions were sampled from mice at 7 and 16 weeks of age, and analysed for free d- and l-amino acids, which are believed to affect many physiological functions. At 7 weeks of age, plasma concentrations of d-aspartic acid (d-Asp), l-alanine (l-Ala), l-glutamine (l-Gln) and taurine were higher in SPF mice than in GF mice, but no differences were found at 16 weeks of age. Similar patterns were observed for the concentrations of l-Asp in striatum, cerebral cortex and hippocampus, and l-arginine (l-Arg), l-Ala and l-valine (l-Val) in striatum. In addition, the concentrations of l-Asp, d-Ala, l-histidine, l-isoleucine (l-Ile), l-leucine (l-Leu), l-phenylalanine and l-Val were significantly higher in plasma of SPF mice when compared with those of GF mice. The concentrations of l-Arg, l-Gln, l-Ile and l-Leu were significantly higher in SPF than in GF mice, but those of d-Asp, d-serine and l-serine were higher in some brain regions of GF mice than in those of SPF mice. In conclusion, the concentration of amino acids in the host brain seems to be dependent on presence of the gut microbiota. Amino acid metabolism in the host brain may be modified by manipulating microbiota communities.. |
| 9. | Hiromi Ikeda, Mao Nagasawa, Takeshi Yamaguchi, Kimie Minaminaka, Ryosei Goda, Vishwajit S. Chowdhury, Shinobu Yasuo, Mitsuhiro Furuse, Disparities in activity levels and learning ability between Djungarian hamster (Phodopus sungorus) and Roborovskii hamster (Phodopus roborovskii), Animal Science Journal, 10.1111/asj.12659, 88, 3, 533-545, 2017.03, [URL], The Djungarian hamster and the Roborovskii hamster belong to the same genus of Phodopus. However, the Djungarian hamster is tame and shows sedative behavior, while Roborovskii hamster is not tame and shows high levels of locomotor activity. Hyperactivity occurs in animals with tameless behavior. Tameness or tamelessness behavior is very important because tameness helps for breeding and controlling as well as it enables a strong human-animal bond. In the present study, we examined the relationships between activity levels and cognitive function in Djungarian and Roborovskii hamsters. Three types of behavioral tests were performed to analyze their activity levels, memory and leaning ability. The levels of L- and D-amino acids and monoamines in the brain were then determined. Roborovskii hamsters showed significantly higher locomotor activity than Djungarian hamsters. Memory ability was not significantly different between the two hamsters, but Roborovskii hamsters showed lower learning ability. Brain levels of D-serine which is related to enhancement in memory and learning ability, were significantly higher in Djungarian hamsters, but the reverse was true for brain dopamine and serotonin levels. These results suggest that these differences in brain metabolism may be related to the behavioral differences between the two hamsters.. |
| 10. | Momoko Kodaira, Mao Nagasawa, Takeshi Yamaguchi, Hiromi Ikeda, Kimie Minaminaka, Vishwajit S. Chowdhury, Shinobu Yasuo, Mitsuhiro Furuse, Aging rather than stress strongly influences amino acid metabolisms in the brain and genital organs of female mice, Mechanisms of Ageing and Development, 10.1016/j.mad.2016.12.006, 162, 72-79, 2017.03, [URL], Aging and stress affect quality of life, and proper nourishment is one of means of preventing this effect. Today, there is a focus on the amount of protein consumed by elderly people; however, changes in the amino acid metabolism of individuals have not been fully considered. In addition, the difference between average life span and healthy life years is larger in females than it is in males. To prolong the healthy life years of females, in the present study we evaluated the influence of stress and aging on metabolism and emotional behavior by comparing young and middle-aged female mice. After 28 consecutive days of immobilization stress, behavioral tests were conducted and tissue sampling was performed. The results showed that the body weight of middle-aged mice was severely lowered by stress, but emotional behaviors were hardly influenced by either aging or stress. Aging influenced changes in amino acid metabolism in the brain and increased various amino acid levels in the uterus and ovary. In conclusion, we found that aged mice were more susceptible to stress in terms of body-weight reduction, and that amino acid metabolisms in the brain and genital organs were largely influenced by aging rather than by stress.. |
| 11. | Takeshi Yamaguchi, Mao Nagasawa, Hiromi Ikeda, Momoko Kodaira, Kimie Minaminaka, Vishwajit S. Chowdhury, Shinobu Yasuo, Mitsuhiro Furuse, Manipulation of dopamine metabolism contributes to attenuating innate high locomotor activity in ICR mice, Behavioural Brain Research, 10.1016/j.bbr.2017.04.001, 328, 227-234, 2017.06, [URL], Attention-deficit hyperactivity disorder (ADHD) is defined as attention deficiency, restlessness and distraction. The main characteristics of ADHD are hyperactivity, impulsiveness and carelessness. There is a possibility that these abnormal behaviors, in particular hyperactivity, are derived from abnormal dopamine (DA) neurotransmission. To elucidate the mechanism of high locomotor activity, the relationship between innate activity levels and brain monoamines and amino acids was investigated in this study. Differences in locomotor activity between ICR, C57BL/6J and CBA/N mice were determined using the open field test. Among the three strains, ICR mice showed the greatest amount of locomotor activity. The level of striatal and cerebellar DA was lower in ICR mice than in C57BL/6J mice, while the level of L-tyrosine (L-Tyr), a DA precursor, was higher in ICR mice. These results suggest that the metabolic conversion of L-Tyr to DA is lower in ICR mice than it is in C57BL/6J mice. Next, the effects of intraperitoneal injection of (6R)-5, 6, 7, 8-tetrahydro-L-biopterin dihydrochloride (BH4) (a co-enzyme for tyrosine hydroxylase) and L-3,4-dihydroxyphenylalanine (L-DOPA) on DA metabolism and behavior in ICR mice were investigated. The DA level in the brain was increased by BH4 administration, but the increased DA did not influence behavior. However, L-DOPA administration drastically lowered locomotor activity and increased DA concentration in several parts of the brain. The reduced locomotor activity may have been a consequence of the overproduction of DA. In conclusion, the high level of locomotor activity in ICR mice may be explained by a strain-specific DA metabolism.. |
| 12. | Mao Nagasawa, Tsuyoshi Otsuka, Yuki Togo, Masakazu Yamanaga, Junki Yoshida, Nobuo Uotsu, Sachiyuki Teramoto, Shinobu Yasuo, Mitsuhiro Furuse, Single and chronic l-serine treatments exert antidepressant-like effects in rats possibly by different means, Amino Acids, 10.1007/s00726-017-2448-8, 49, 9, 1561-1570, 2017.09, [URL], In the present study, the effects of both single (6 mmol l-serine/10 ml/kg orally administrated) and chronic (2% l-serine solution freely given for 28 days) treatments on depression-like behavior were evaluated in Wistar rats, representing the control, and Wistar Kyoto rats, representing an animal model of depression. Both single and chronic l-serine treatments decreased the duration of immobility, which is an index of a depressive-like state, in the forced swimming test in both strains. However, the decreases in the duration of immobility appear to be regulated differently by the different mechanisms involved in single and chronic l-serine treatments. In the prefrontal cortex and hippocampus, single l-serine treatment increased the concentrations of l-serine, but not d-serine, while chronic l-serine treatment increased those of d-serine, but not l-serine. These data suggest that the antidepressant-like effects of single and chronic l-serine treatments may have been induced by the increased l-serine and d-serine concentrations, respectively, in the brain. In addition, chronic l-serine treatment increased cystathionine concentrations in the hippocampus and prefrontal cortex in Wistar rats, but not in Wistar Kyoto rats, suggesting that Wistar Kyoto rats have an abnormality in the serine–cystathionine metabolic pathway. In conclusion, single and chronic l-serine treatments may induce antidepressant-like effects via the different mechanisms related to serine metabolism in the brain.. |
| 13. | Tkkuma Nishigawa, Satsuki Nagamachi, Mayumi Takakura, Hiromi Ikeda, Momoko Kodaira, Takeshi Yamaguchi, Vishwajit Surchowdhury, Shinobu Yasuo, Mitsuhiro Furuse, Maternal stress during the lactation period rather than the gestation period strongly influences the amino acid composition in milk and affects growth and behaviour in offspring, Journal of the Faculty of Agriculture, Kyushu University, 63, 1, 61-70, 2018.02, It is well known that maternal stress during the gestation and lactation periods induces abnormal behavior in the offspring and causes a lowering of the offspring's body weight. However, the negative effects of maternal stress on the mother's milk, which is one of the most important connecting factors between mothers and their offspring, are not yet fully understood. The present study aimed to investigate how free amino acids in milk, as well as the growth and behavior of offspring, are changed by maternal stress during the gestation and lactation periods. Although maternal stress during the gestation period did not affect the growth of offspring or the free amino acid composition in the milk, the offspring in the stressed group showed lower locomotor activity. However, maternal stress during the lactation period caused a reduction in body weight in the offspring following the stress load. It increased the concentration in the milk of several amino acids, such as taurine, aspartic acid and β-alanine, while it decreased the concentration of most of the free amino acids in the maternal plasma. Notably, the body weight of offspring at postnatal age 11 days was negatively correlated with taurine concentration in the milk. These results show that maternal stress during the lactation period may have a great impact on the free amino acid composition of the milk, which may partly, but not entirely, be the cause of lower body weight in the offspring.. |
| 14. | Satsuki Nagamachi, Takuma Nishigawa, Mayumi Takakura, Hiromi Ikeda, Momoko Kodaira, Takeshi Yamaguchi, Vishwajit Sur Chowdhury, Shinobu Yasuo, Mitsuhiro Furuse, Dietary L-serine modifies free amino acid composition of maternal milk and lowers the body weight of the offspring in mice, Journal of Veterinary Medical Science, 10.1292/jvms.17-0577, 80, 2, 235-241, 2018.02, [URL], The growth of offspring is affected not only by the protein in maternal milk but also by the free amino acids (FAAs) contained in it. L-Serine (L-Ser) is known as an important FAA for the development of the central nervous system and behavioral activity. However, it is not clear whether L-Ser is transported into the pool of FAAs contained in milk and thereby affects the growth of offspring. Using mice, the current study investigated the effects of dietary L-Ser during pregnancy and lactation on milk and plasma FAA composition, as well as on growth, behavior, and plasma FAAs of offspring. Dietary L-Ser did not significantly affect the maternal, anxiety-like, or cognitive behaviors of either the dam or the offspring. The FAA composition notably differed between plasma and milk in dams. In milk, dietary L-Ser increased free L-Ser levels, while glutamic acid, L-alanine, D-alanine and taurine levels were decreased. The body weight of the offspring was lowered by dietary L-Ser. The concentrations of plasma FAAs in 13-day-old offspring (fed only milk) were not altered, but 20-day-old offspring (fed both milk and parental diet) showed higher plasma L-Ser and D-Ser concentrations as a result of the dietary L-Ser treatment. In conclusion, the present study found that dietary L-Ser transported easily from maternal plasma to milk and that dietary L-Ser treatment could change the FAA composition of milk, but that an enhanced level of L-Ser in milk did not enhance the plasma L-Ser level in the offspring.. |
| 15. | Naho Morisaki, Chie Nagata, Shinobu Yasuo, Seiichi Morokuma, Kiyoko Kato, Masafumi Sanefuji, Eiji Shibata, Mayumi Tsuji, Ayako Senju, Toshihiro Kawamoto, Shoichi Ohga, Koichi Kusuhara, Optimal protein intake during pregnancy for reducing the risk of fetal growth restriction The Japan Environment and Children's Study, British Journal of Nutrition, 10.1017/S000711451800291X, 120, 12, 1432-1440, 2018.12, [URL], Clinical trials show that protein supplement increases infant size in malnourished populations; however, epidemiological studies in high-income countries have reported mixed results. Although these findings suggest a non-linear relationship between maternal macronutrient intake and fetal growth, this relationship has not been closely examined. We assessed the association between maternal protein intake and fetal growth among 91 637 Japanese women with singletons in a nation-wide cohort study using validated FFQ. The respondents answered the FFQ twice, once during early pregnancy (FFQ1; 16·3 (sd 6·0) weeks), and second during mid-pregnancy (FFQ2, 28·1 (sd 4·1) weeks). Daily energy intake and percentage energy from protein, fats and carbohydrates were 7477 (sd 2577) kJ and 13·5 (sd 2·0), 29·5 (sd 6·5) and 55·3 (sd 7·8) %, respectively, for FFQ1, and 7184 (sd 2506) kJ and 13·6 (sd 2·1), 29·8 (sd 6·6) and 55·3 (sd 7·9) %, respectively, for FFQ2. The average birth weight was 3028 (sd 406) g, and 6350 infants (6·9 %) were small for gestational age (SGA). In both phases of the survey, birth weight was highest and the risk of SGA was lowest when the percentage energy from protein was 12 %, regardless of whether isoenergetic replacement was with fat or carbohydrates. Furthermore, when protein density in the maternal diet was held constant, birth weight was highest when 25 % of energy intake came from fat and 61 % came from carbohydrates during early pregnancy. We found maternal protein intake to have an inverse U-curve relationship with fetal growth. Our results strongly suggest that the effect of protein on birth weight is non-linear, and that a balanced diet fulfilling the minimum requirement for all macronutrients was ideal for avoiding fetal growth restriction.. |
| 16. | Yusuke Takaia, Misato Kawaia, Tadashi Ogob, Takashi Ichinoseb, Shigeki Furuyab, Nozomu Takakia, Yukihiro Tonea, Hiroshi Udoc, Mitsuhiro Furusea, Shinobu Yasuoa, Early-life photoperiod influences depression-like behavior, prepulse inhibition of the acoustic startle response, and hippocampal astrogenesis in mice, Neuroscience, 10.1016/j.neuroscience.2018.01.038, 374, 133-143, 2018.03. |
| 17. | Shinobu Yasuo, Ayaka Iwamoto, Sang-il Lee, Shotaro Ochiai, Rina Hitachi, Satomi Shibata, Nobuo Uotsu, Chie Tarumizu, Sayuri Matsuoka, Mitsuhiro Furuse, Shigekazu Higuchi, L-Serine enhances light-induced circadian phase resetting in mice and humans, Journal of Nutrition, 10.3945/jn.117.255380, 147, 12, 2347-2355, 2017.12, 約24時間のリズムを刻む概日時計（体内時計）は食事の時刻や内容により調節される。しかし、特定の栄養素が概日時計に及ぼす影響については不明な点が多い。本研究では、概日時計に影響を及ぼすアミノ酸を同定することを目的とした。 CBA/Nマウスにおいて、回転輪活動リズムの位相変化量を指標として解析を行った。20種類のアミノ酸のうち、L-セリンを投与したマウスでは、光による概日時計の位相変化が強まることが判明した。この効果はGABA-A受容体アンタゴニストであるピクロトキシンにより阻害された。また、明暗周期を6時間前進させてL-セリンを経口投与したところ、新しい明暗周期に対する再同調が早まった。 L-セリンの効果をヒトで実証するため、L-セリンが光による概日時計の位相前進に及ぼす影響について、男子大学生を対象として解析した。L-セリンを就寝前に摂取して翌朝に強い光を浴びると、概日時計の位相の指標であるメラトニン分泌開始時刻が有意に大きく前進した。 以上の結果から、光による概日時計のリセットがL-セリンの摂取により強まることが解明された。本成果により、概日時計の乱れや時差ぼけの改善にL-セリンが有用である可能性が示唆された。. |
| 18. | Ayako Tashiro, Satomi Shibata, Yusuke Takai, Tatsuhiro Uchiwa, Mitsuhiro Furuse, Shinobu Yasuo, Changes in photoperiod alter Glut4 expression in skeletal muscle of C57BL/6J mice, Biochemical and Biophysical Research Communications, 485, 82-88, 2017.03. |
| 19. | Tatsuhiro Uchiwa, Yusuke Takai, Ayako Tashiro, Mitsuhiro Furuse, Yasuo Shinobu, Exposure of C57BL/6J mice to long photoperiod during early life stages increases body weight and alters plasma metabolomic profiles in adulthood, Physiological Reports, 4, e12974, 2016.09. |
| 20. | Shoko Furuta, Rika Kuwahara, Eri Hiraki, Koichiro Ohnuki, Shinobu Yasuo, Kuniyoshi Shimizu, Hericium erinaceus extracts alter behavioral rhythm in mice, Biomedical Research, 37, 227-232, 2016.03. |
| 21. | Tsuyoshi Otsuka, Ryosei Goda, Ayaka Iwamoto, Misato Kawai, Satori Shibata, Yoshiaki Oka, Wataru Mizunoya, Mitsuhiro Furuse, Yasuo Shinobu, Dietary protein ingested before and during short photoperiods makes an impact on affect-related behaviours and plasma composition of amino acids in mice, British Journal of Nutrition, 114, 1734-1743, 2015.11. |
| 22. | Misato Kawai, Ryosei Goda, Tsuyoshi Otsuka, Ayaka Iwamoto, Nobuo Uotsu, Mitsuhiro Furuse, Yasuo Shinobu, Antidepressant-like effect of bright light is potentiated by L-serine administration in a mouse model of seasonal affective disorder, Brain Research Bulletin, 118, 25-33, 2015.09. |
| 23. | Ryosei Goda, Tsuyoshi Otsuka, Ayaka Iwamoto, Misato Kawai, Satomi Shibata, Mitsuhiro Furuse, Yasuo Shinobu, Serotonin levels in the dorsal raphe nuclei of both chipmunks and mice are enhanced by long photoperiod, but brain dopamine level response to photoperiod is species-specific, Neuroscience Letters, 593, 95-100, 2015.04. |
| 24. | Andras D. Nagy, Ayaka Iwamoto, Misato Kawai, Ryosei Goda, Haruka Matsuo, Tsuyoshi Otsuka, Mao Nagasawa, Mitsuhiro Furuse, Yasuo Shinobu, Melatonin adjusts the expression pattern of clock genes in the suprachiasmatic nucleus and induces antidepressant-like effect in a mouse model of seasonal affective disorder, Chronobiology International, 32, 447-457, 2015.05. |
| 25. | Haruka Matsuo, Ayaka Iwamoto, Tsuyoshi Otsuka, Yukihiro Hishida, Saori Akiduki, Mami Aoki, Shozo Tomonaga, Mitsuhiro Furuse, Yasuo Shinobu, Effects of time of l-ornithine administration on the diurnal rhythms of plasma growth hormone, melatonin, and corticosterone levels in mice, Chronobiology International, 32, 225-234, 2015.03. |
| 26. | Yasuo Shinobu, Claudia Fischer, Joerg Bojunga, Masayuki Iigo, Horst-Werner Korf, 2-Arachidonoyl glycerol sensitizes the pars distalis and enhances forskolin-stimulated prolactin secretion in Syrian hamsters, Chronobiology International, 31, 337-342, 2014.04. |
| 27. | Tsuyoshi Otsuka, Misato Kawai, Yuki Togo, Ryosei Goda, Takahiro Kawase, Haruka Matsuo, Ayaka Iwamoto, Mao Nagasawa, Furuse Mitsuhiro, Yasuo Shinobu, Photoperiodic responses of depression-like behavior, the brain serotonergic system, and peripheral metabolism in laboratory mice, Psychoneuroendocrinology, 40, 37-47, 2014.02. |
| 28. | Ayaka Iwamoto, Misato Kawai, Furuse Mitsuhiro, Yasuo Shinobu, Effects of chronic jet lag on the central and peripheral circadian clocks in CBA/N mice, Chronobiology International, 31, 189-198, 2014.03. |
| 29. | Goto Mariko, Matsuo Haruka, Iigo Masayuki, Furuse Mitsuhiro, Horst-Werner Korf, Yasuo Shinobu, Melatonin-induced changes in the expression of thyroid hormone-converting enzymes in hypothalamus depend on the timing of melatonin injections and genetic background in mice., General and Comparative Endocrinology, 10.1016/j.ygcen.2013.02.028, 186C, 33-40, 2013.03. |
| 30. | Togo Yuki, Otsuka Tsuyoshi, Goto Mariko, Furuse Mitsuhiro, Yasuo Shinobu, Photoperiod regulates dietary preferences and energy metabolism in young developing Fischer 344 rats but not in same-age Wistar rats, American Journal of Physiology-Endocrinology and Metabolism, 10.1152/ajpendo.00209.2012, 303, 6, E777-E786, 2012.09. |
| 31. | Otsuka Tsuyoshi, Goto Mariko, Kawai Misato, Togo Yuki, Sato Katsuyoshi, Katoh Kazuo, Furuse Mitsuhiro, Yasuo Shinobu, Photoperiod regulates corticosterone rhythms by altered adrenal sensitivity via melatonin-independent mechanisms in Fischer 344 rats and C57BL/6J mice, PLoS ONE, 10.1371/journal.pone.0039090, 7, 6, e39090, 2012.06. |
| 32. | Shinobu Yasuo, Claudia Unfried, Matthias Kettner, Gerd Geisslinger, Horst-Werner Korf, Localization of an endocannabinoid system in the hypophysial pars tuberalis and pars distalis of man, Cell and Tissue Research, 342: 273-281, 2010.11. |
| 33. | Shinobu Yasuo, Marco Koch, Helmut Schmidt, Simone Ziebell, Joerg Bojung, Gerd Geisslinger, Horst-Werner Korf, An endocannabinoid system is localized to the hypophysial pars tuberalis of Syrian hamsters and responds to photoperiodic changes, Cell and Tissue Research, 340:127-136, 2010.04. |
| 34. | Shinobu Yasuo, Takashi Yoshimura, Shizufumi Ebihara, Horst-Werner Korf, Photoperiodic control of TSH-b expression in the mammalian pars tuberalis has different impact on the induction and suppression of hypothalamo-hypophysial gonadal axis, Journal of Neuroendocrinology, 22: 43-50, 2010.01. |
| 35. | Hiroko Ono, Hoshino Yuta, Shinobu Yasuo, Miwa Watanabe, Nakane Yusuke, Atsushi Murai, Shizufumi Ebihara, Horst-Werner Korf, Takashi Yoshimura, Involvement of thyrotropin in photoperiodic signal transduction in mice, Proceedings of the National Academy of Sciences of the United States of America, 105, 18238-18242 , 2009.11. |
| 36. | Claudia Unfried, Nariman Ansari, Shinobu Yasuo, Horst-Werner Korf, Charlotte von Gall, Impact of melatonin and molecular clockwork components on the expression of thyrotropin-beta chain (Tshb) and the Tsh receptor in the mouse pars tuberalis, Endocrinology, 150, 4653-4662, 2009.10. |
| 37. | Shinobu Yasuo, Takashi Yoshimura, Shizufumi Ebihara, Horst-Werner Korf, Melatonin transmits photoperiodic signals through the MT1 melatonin receptor, Journal of Neuroscience, 29, 2885-2889 , 2009.03. |
| 38. | Tsuyoshi Watanabe, Suzuki Tohru, Akira Ishikawa, Yuki Yokota, Hiroki R. Ueda, Rikuhiro G. Yamada, Hajime Tei, Saki Imai, Shigeru Tomida, Junya Kobayashi, Emiko Naito, Shinobu Yasuo, Nobuhiro Nakao, Takao Namikawa, Takashi Yoshimura, Shizufumi Ebihara, Genetic and Molecular Analysis of wild-derived arrhythmic mice, PLoS ONE, e4301, 2009.01. |
| 39. | Shinobu Yasuo, Charlotte von Gall, David R Weaver, Horst-Werner Korf, Rhythmic expression of clock genes in the ependymal cell layer of the third ventricle of rodents is independent of melatonin signaling, European Journal of Neuroscience, 28, 2443-2450 , 2008.12. |
| 40. | Nobuhiro Nakao, Hiroko Ono, Takashi Yamamura, Tsubasa Anraku, Tsuyoshi Takagi, Kumiko Higashi, Shinobu Yasuo, Yasuhiro Katou, Saburo Kageyama, Yumiko Uno, Takeya Kasukawa, Masayuki Iigo, Peter J. Sharp, Atsushi Iwasawa, Yutaka Suzuki, Sumio Sugano, Teruyuki Niimi, Makoto Mizutani, Takao Namikawa, Shizufumi Ebihara, Hiroki R. Ueda, Takashi Yoshimura, Thyrotrophin in the pars tuberalis triggers photoperiodic response, Nature, 452, 317-322, 2008.03. |
| 41. | Shinobu Yasuo, Takashi Yoshimura, Shizufumi Ebihara, Horst-Werner Korf, Temporal dynamics of type 2 deiodinase expression after melatonin injections in Syrian hamsters, Endocrinology, 148, 4385-4392, 2007.09. |
| 42. | Tsubasa Anraku, Tsuyoshi Takagi, Nobuhiro Nakao, Miwa Watanabe, Shinobu Yasuo, Yasuhiro Katou, Yukihiro Ueda, Atsushi Murai, Masayuki Iigo, Shizufumi Ebihara, Takashi Yoshimura, Photoperiodic changes in hypothalamic insulin receptor gene expression are regulated by gonadal testosterone, Brain Research, 1163, 86-90 , 2007.08. |
| 43. | Nobuhiro Nakao, Shinobu Yasuo, Atsuko Nishimura, Takashi Yamamura, Tuyoshi Watanabe, Tubasa Anraku, Toshiyuki Okano, Yoshitaka Fukada, Peter J. Sharp, Shizufumi Ebihara, Takashi Yoshimura, Circadian clock gene regulation of steroidogenic acute regulatory protein gene expression in pre-ovulatory ovarian follicles, Endocrinology, 148, 3031-3038 Selected as News & Views: Ball GF, The ovary knows more than you think! New views on clock genes and the positive feedback control of luteinizing hormone. Endocrinology 148, 3029-3030 (2007), 2007.07. |
| 44. | Yasuhiro Aoki, Hiroko Ono, Shinobu Yasuo, Tomohiro Masuda, Takashi Yoshimura, Shizufumi Ebihara, Masayuki Iigo, Tadashi Yanagisawa, Molecular evolution of prepro-thyrotropin-releasing hormone in the chicken (Gallus gallus) and its expression in the brain, Zoological Science, 24, 686-692 , 2007.07. |
| 45. | Tsuyoshi Takagi, Takashi Yamamura, Tubasa Anraku, Shinobu Yasuo, Nobuhiro Nakao, Miwa Watanabe, Masayuki Iigo, Shizufumi Ebihara, Takashi Yoshimura, Involvement of Transforming Growth Factor {alpha} in the Photoperiodic Regulation of Reproduction in Birds, Endocrinology, 148, 2788-2792, 2007.06. |
| 46. | Shinobu Yasuo, Miwa Watanabe, Masayuki Iigo, Takahiro J. Nakamura, Tsuyoshi Watanabe, Tsuyoshi Takagi, Hiroko Ono, Shizufumi Ebihara, Takashi Yoshimura, Differential response of type 2 deiodinase gene expression to photoperiod between photoperiodic Fischer 344 and nonphotoperiodic Wistar rats, American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 292, R1315-1319, 2007.03. |
| 47. | Tsuyoshi Watanabe, Takashi Yamamura, Miwa Watanabe, Shinobu Yasuo, Nobuhiro Nakao, Alistair Dawson, Shizufumi Ebihara, Takashi Yoshimura, Hypothalamic expression of thyroid hormone-activating and –inactivating enzyme genes in relation to photorefractoriness in birds and mammals, American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 292, R568-572, 2007.01. |
| 48. | Takahi Yamamura, Shinobu Yasuo, Kanjun Hirunagi, Shizufumi Ebihara, Takashi Yoshimura, T3 implantation mimics photoperiodically reduced encasement of nerve terminals by glial processed in the median eminence of Japanese quail, Cell and Tissue Reserch , 324, 175-179 , 2006.04. |
| 49. | Tsuyoshi Watanabe, Hitomi Kojima, Shigeru Tomida, Takahiro J Nakamura, Nobuhiro Nakao, Shinobu Yasuo, Takashi Yoshimura, Shizufumi Ebihara, Peripheral clock gene expression in CS mice with bimodal locomotor rhythms, Neuroscience Research, 54, 295-301, 2006.04. |
| 50. | Nobuhiro Nakao, Tsuyoshi Takagi, Masayuki Iigo, Toshiro Tsukamoto, Shinobu Yasuo, Tomohiro Masuda, Tadashi Yanagisawa, Shizufumi Ebihara, Takashi Yoshimura, Possible involvement of organic anion transporting polypeptide (Oatp) 1c1 in the photoperiodic response of gonads in birds, Endocrinology, 147, 1067-1073 Selected as News & Views: Ball GF, Thyroid hormone transport and photoperiodism: feeling one's oatps. Endocrinology 147, 1065-1066 (2003), 2006.03. |
| 51. | Shinobu Yasuo, Nobuhiro Nakao, Satoshi Ohkura, Masayuki Iigo, Satoko Hagiwara, Akemitsu Goto, Hiroshi Ando, Takashi Yamamura, Miwa atanabe, Tsuyoshi Watanabe, Sen-ichi Oda, Kei-ichiro Maeda, Gerald Lincoln, Hiroaki Okamura, Shizuhumi Ebihara, Takashi Yoshimura, Long day suppressed expression of type 2 deiodinase gene in the mediobasal hypothalamus of the Saanen goat, a short day breeder: Implication for seasonal window of thyroid hormone action on reproductive neuroendocrine axis, Endocrinology, 147, 432-440 , 2006.01. |
| 52. | Mayumi Tsuchimoto, Shinobu Yasuo, Masahiro Funada, Makoto Aoki, Hiromi Sasagawa, Sydney A. Cameron, Yasuo Kitagawa, Tatsuhiko Kadowaki, Conservation of novel Mahya genes shows the existence of neural functions common between Hymenoptera and Deuterostome, Development Genes and Evolution , 10.1007/s00427-005-0021-z, 215, 11, 564-574, 215, 432-440 , 2005.11. |
| 53. | Shinobu Yasuo, Miwa Watanabe, Nobuhiro Nakao, Tsuyoshi Takagi, Brian K. Follett, Shizufumi Ebihara, Takashi Yoshimura, The reciprocal switching of two thyroid hormone-activating and –inactivating enzyme genes is involved in the photoperiodic gonadal response of Japanese quail, Endocrinology, 146, 2551-2554 , 2005.06. |
| 54. | Shinobu Yasuo, Shizuhumi Ebihara, Takashi Yoshimura, Oral thyroxine administration mimics photoperiodically induced gonadal growth in Japanese quail, Animal Science Journal , 75, 407-410 , 2004.10. |
| 55. | Miwa Watanabe, Shinobu Yasuo, Takashi Yamamura, Nobuhiro Nakao, Shizufumi Ebihara, Takashi Yoshimura , Photoperiodic regulation of type 2 deiodinase gene in Djungarian hamster: possible homologies between avian and mammalian photoperiodic regulation of reproduction, Endocrinology, 145, 1546-1549, 2004.04. |
| 56. | Shinobu Yasuo, Miwa Watanabe, Akira Tsukada, Tsuyoshi Takagi, Kiyoshi Shimada, Shizufumi Ebihara, Takashi Yoshimura, Photoinducible phase specific light induction of Cry1 gene in the pars tuberalis of Japanese quail, Endocrinology, 145, 1612-1616, 2004.04. |
| 57. | Takashi Yoshimura, Yuki Yokota, Akira Ishikawa, Shinobu Yasuo, Noriko Hayashi, Tohru Suzuki, Naritoshi Okabayashi, Takao Namikawa, Shizufumi Ebihara, Mapping quantitative trait loci affecting circadian photosensitivity in retinally degenerate mice, Journal of Biological Rhythms, 17, 512-519 , 2002.12. |
| 58. | Shinobu Yasuo, Miwa Watanabe, Naritoshi Okabayashi, Shizufumi Ebihara, Takashi Yoshimura, Circadian clock genes and photoperiodism: Comprehensive analysis of clock genes expression in the mediobasal hypothalamus, the suprachiasmatic nucleus and the pineal gland of Japanese quail under various light schedules, Endocrinology, 144, 3742-3748 Selected as News & Views: Ball GF and Balthazart J, Birds return every spring like clockwork, but where is the clock? Endocrinology 144, 3739-3741(2003), 2003.09. |
| 59. | Takashi Yoshimura, Shinobu Yasuo, Miwa Watanabe, Masayuki Iigo, Takashi Yamamura, Kanjun Hirunagi, Shizufumi Ebihara, Light-induced hormone conversion of T4 to T3 regulates photoperiodic response of gonads in birds, Nature, 426, 178-181, 2003.11. |
| 60. | Naritoshi Okabayashi, Shinobu Yasuo, Miwa Watanabe, Takao Namikawa, Shizufumi Ebihara, Takashi Yoshimura, Ontogeny of circadian clock gene expression in the pineal and the suprachiasmatic nucleus of chick embryo, Brain Research, 990, 231-234 , 2003.11. |
| 61. | Noriko Hayashi, Shinobu Yasuo, Shizufumi Ebihara, Takashi Yoshimura , Expression of IKKalpha mRNA in the suprachiasmatic nucleus and circadian rhythms of mice lacking IKKalpha, Brain Research, 993, 217-221, 2003.12. |
| 62. | Masahiro Funada, Shinobu Yasuo, Takashi Yoshimura,, Shizuhumi Ebihara, Hiromi Sasagawa, Yasuo Kitagawa, Tatsuhiko Kadowaki, Characterization of the two distinct subtypes of metabotropic glutamate receptors from honeybee, Apis mellifera, Neuroscience Letters, 359, 190-104 , 2004.04. |
| 63. | Shinobu Yasuo, Shizufumi Ebihara, Takashi Yoshimura, Circadian expression of clock gene in the optic tectum of Japanese quail, Brain Research, 1005, 193-196 , 2004.04. |
| 64. | Shinobu Yasuo, Takashi Yoshimura, Paul A. Bartell, Masayuki Iigo, Eri Makino, Naritoshi Okabayashi, Shizufumi Ebihara, Effect of melatonin administration on qPer2, qPer3, and qClock gene expression in the suprachiasmatic nucleus of Japanese quail, European Journal of Neuroscience, 16, 1541-1546 , 2002.10. |
| 65. | Takashi Yoshimura, Shinobu Yasuo, Yoshikazu Suzuki, Eri Makino, Yuki Yokota, Shizufumi Ebihara, Identification of the suprachiasmatic nucleus in birds, American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 292, R1185-1189, 2001.04. |
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