Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Motohiro Tani Last modified date:2023.04.24

Associate Professor / Organic and Biological Chemistry / Department of Chemistry / Faculty of Sciences

1. Koga A, Takayama C, Ishibashi Y, Kono Y, Matsuzaki M, and Tani M., Loss of tolerance to multiple environmental stresses due to limitation of structural diversity of complex sphingolipids., Mol Biol Cell, DOI: 10.1091/mbc.E22-04-0117, 33, 12, ar105, 2022.08, Structural diversity of complex sphingolipids is important for maintenance of various cellular functions; however, the overall picture of the significance of this structural diversity remains largely unknown. To investigate the physiological importance of the structural diversity of complex sphingolipids, we here constructed a complex sphingolipid structural diversity disruption library in budding yeast, which comprises 11 mutants including with combinations of deletions of sphingolipid-metabolizing enzyme genes. The sensitivity of the mutants to various environmental stresses revealed that the more the structural variation of complex sphingolipids is limited, the more stress sensitivity tends to increase. Moreover, it was found that in mutant cells with only one subtype of complex sphingolipid, Slt2 MAP kinase and Msn2/4 transcriptional factors are essential for maintenance of a normal growth and compensation for reduced tolerance of multiple stresses caused by loss of complex sphingolipid diversity. Slt2 and Msn2/4 are involved in compensation for impaired integrity of cell walls and plasma membranes caused by loss of complex sphingolipid diversity, respectively. From these findings, it was suggested that loss of structural diversity of complex sphingolipids affects the environment of the cell surface, including both plasma membranes and cell walls, which could cause multiple environmental stress hypersensitivity..
2. Takayama C, Koga A, Sakamoto R, Arita N, and Tani M., Involvement of the mitochondrial retrograde pathway in dihydrosphingosine-induced cytotoxicity in budding yeast, Biochem Biophys Res Commun., DOI: 10.1016/j.bbrc.2022.03.061, 605, 63-69, 2022.03.
3. Urita A, Ishibashi Y, Kawaguchi R, Yanase Y, and Tani M., Crosstalk between protein kinase A and the HOG pathway under impaired biosynthesis of complex sphingolipids in budding yeast., FEBS J., DOI: 10.1111/febs.16188, 289, 766-786, 2022.01.
4. Ishino Y, Komatsu N, Sakata K, Yoshikawa D, Tani M, Maeda T, Morishige K, Yoshizawa K, Tanaka N, and Tabuchi M, Regulation of sphingolipid biosynthesis in the endoplasmic reticulum via signals from the plasma membrane in budding yeast., FEBS J., DOI: 10.1111/febs.16189, 289, 457-472, 2022.01.
5. Kurauchi T, Matsui K, Shimasaki T, Ohtsuka H, Tsubouchi S, Ihara K, Tani M, and Aiba H, Identification of sur2 mutation affecting the lifespan of fission yeast., FEMS Microbiol Lett., DOI: 10.1093/femsle/fnab070, 368, fnab070, 2021.05.
6. Toda T, Urita A, Koga A, Takayama C, Tani M, ROS-mediated synthetic growth defect caused by impaired metabolism of sphingolipids and phosphatidylserine in budding yeast., Biosci Biotechnol Biochem., DOI: 10.1080/09168451.2020.1810539, 84, 2529-2532, 2020.08.
7. Otsu M, Toume M, Yamaguchi Y, Tani M, Proper regulation of inositolphosphorylceramide levels is required for acquirement of low pH resistance in budding yeast., Sci Rep., DOI: 10.1038/s41598-020-67734-8, 10, 10792, 2020.07.
8. Arita N, Sakamoto R, Tani M, Mitochondrial reactive oxygen species-mediated cytotoxicity of intracellularly accumulated dihydrosphingosine in the yeast Saccharomyces cerevisiae, FEBS J., DOI: 10.1111/febs.15211, 287, 3427-3448, 2020.08.
9. Tanaka S, Tani M, Mannosylinositol phosphorylceramides and ergosterol coodinately maintain cell wall integrity in the yeast Saccharomyces cerevisiae, FEBS J., DOI: 10.1111/febs.14509, 2018.05, In the yeast Saccharomyces cerevisiae, complex sphingolipids have three types of polar head group, and breakdown of the normal composition causes several cellular dysfunctions. Previously we found that loss of biosynthesis of mannosylinositol phosphorylceramide (MIPC) causes a defect in cell wall integrity. In this study, we screened for multicopy suppressor genes that rescue the defect in cell wall integrity in cells lacking MIPC synthases (Sur1 and Csh1), and found that the defect is partly suppressed by upregulation of ergosterol biosynthesis. In addition, repression of expression of ERG9, which encodes squalene synthase in the ergosterol biosynthesis pathway, in sur1∆ csh1∆ cells caused a strong growth defect and enhancement of the defect in cell wall integrity. The repression of ERG9 and/or the deletion of SUR1 and CSH1 caused an increase in the phosphorylated form of Slt2, a mitogen-activated protein (MAP) kinase activated through impairment of cell wall integrity. Moreover, the deletion of SLT2 or WSC1/2 encoding a sensor protein recognizing cell wall integrity enhanced the growth defect in the ERG9-repressed sur1∆ csh1∆ cells. On the other hand, the ERG9-repressed sur1∆ csh1∆ cells also exhibited an increase in the cell-wall chitin level in a Slt2- and Wsc1/2-independent manner. These results suggested that MIPC and ergosterol are coordinately involved in maintenance of cell wall integrity, and the activation of Slt2 suppress the cell wall integrity defect caused by these metabolic defects..
10. Katsuki Y, Yamaguchi Y, Tani M, Overexpression of PDR16 confers resistance to complex sphingolipid biosynthesis inhibitor aureobasidin A in yeast Saccharomyces cerevisiae, FEMS Microbiol Lett., DOI: 10.1093/femsle/fnx255, 365, fnx255, 2018.02.
11. Yamaguchi Y, Katsuki Y, Tanaka S, Kawaguchi R, Denda H, Ikeda T, Funato K, Tani M, Protective role of the HOG pathway against the growth defect caused by impaired biosynthesis of complex sphingolipids in yeast Saccharomyces cerevisiae., Mol Microbiol., DOI: 10.1111/mmi.13886, 107, 3, 363-386, 2018.01.
12. Toume M, Tani M, Yeast lacking the amphiphysin-family protein Rvs167 is sensitive to disruptions in sphingolipid levels., FEBS J., DOI: 10.1111/febs.13783, 283, 2911-2928, 2016.06.
13. Sakakibara K, Eiyama A, Suzuki SW, Sakoh-Nakatogawa M, Okumura N, Tani M, Hashimoto A, Nagumo S, Kondo-Okamoto N, Kondo-Kakuta C, Asai E, Kirisako H, Nakatogawa H, Kuge O, Takao T, Ohsumi Y, Okamoto K, Phospholipid methylation controls Atg32-mediated mitophagy and Atg8 recycling, EMBO J., 34, 21, 2703-2719, 2015.10.
14. Miyata N, Miyoshi T, Yamaguchi T, Nakazono T, Tani M, Kuge O, VID22 is required for transcriptional activation of the PSD2 gene in the yeast Saccharomyces cerevisiae., Biochem J., 472, 319-328, 2015.10.
15. Ban-Ishihara R, Tomohiro-Takamiya S, Tani M, Baudier J, Ishihara N, Kuge O, COX assembly factor ccdc56 regulates mitochondrial morphology by affecting mitochondrial recruitment of Drp1., FEBS Lett., 2015.09.
16. Tani M, Toume M, Alteration of complex sphingolipid composition and its physiological significance in yeast Saccharomyces cerevisiae lacking vacuolar ATPase., Microbiology-Sgm, DOI: 10.1099/mic.0.000187, 161, 2369-2383, 2015.09.
17. Watanebe T, Tani M, Ishibashi Y, Endo I, Okino N, Ito M, Ergosteryl-β-glucosidase (Egh1) involved in sterylglucoside catabolism and vacuole formation in Saccharomyces cerevisiae., Glycobiology, 25, 10, 1079-1089, 2015.06.
18. Morimoto Y, Tani M, Synthesis of mannosylinositol phosphorylceramides is involved in maintenance of cell integrity of yeast Saccharomyces cerevisiae, Mol Microbiol, DOI: 10.1111/mmi.12896, 95, 4, 706-722, 2015.02.
19. Toume M, Tani M, Change in activity of serine palmitoyltransferase affects sensitivity to syringomycin E in yeast Saccharomyces cerevisiae., FEMS Microbiol Lett., DOI: 10.1111/1574-6968.12535, 358, 64-71, 2014.07.
20. Uemura S, Shishido F, Tani M, Mochizuki T, Abe F, Inokuchi J, Loss of hydroxyl groups from the ceramide moiety can modify the lateral diffusion of membrane proteins in Saccharomyces cerevisiae., J Lipd Res, 55, 1343-1356, 2014.05.
21. Tani M, Kuge O, Involvement of Sac1 phosphoinositide phosphatase in metabolism of phosphatidylserine in the yeast Saccharomyces cerevisiae., Yeast, DOI: 10.1002/yea.3004, 31, 145-158, 2014.02.
22. Tani M, Kuge O, Involvement of complex sphingolipids and phosphatidylserine in endosomal trafficking in yeast Saccharomyces cerevisiae., Mol Microbiol., DOI: 10.1111/mmi.12057, 86, 5, 1262-1280, 2012.10.
23. Nakase M, Tani M, and Takegawa K., Expression of budding yeast IPT1 produces mannosyldiinositolphosphorylceramide in fission yeast and inhibits cell growth., Microbiology, 158, 1219-1228, 2012.02.
24. Tani M, and Kuge O., Hydroxylation state of fatty acid and long-chain base moieties of sphingolipid determine the sensitivity to growth inhibition due to AUR1 repression in Saccharomyces cerevisiae, Biochem Biophys Res Commun., DOI: 10.1016/j.bbrc.2011.11.138, 417, 673-678, 2012.01.
25. Nakagawa T, Tani M, Sueyoshi N, Ito M, The mucin box and signal/anchor sequence of rat neutral ceramidase recruit bacterial sphingomyelinase to the plasma membrane., Biosci Biotechnol Biochem., 75, 5, 2011.05.
26. Kuroda T, Tani M, Moriguchi A, Tokunaga S, Higuchi T, Kitada K, Kuge O, FMP30 is required for the maintenance of a normal cardiolipin level and mitochondrial morphology in the absence of mitochondrial phosphatidylethanolamine synthesis., Mol. Microbiol., 80, 1, 248-265, 2011.02.
27. Tani M, and Kuge O, Defect of synthesis of very long-chain fatty acids confers resistance to growth inhibition by inositol phosphorylceramide synthase repression in yeast Saccharomyces cerevisiae., J. Biochem., DOI: 10.1093/jb/mvq090, 148, 5, 565-571, 2010.08.
28. Tani M, and Kuge O, Requirement of a specific group of sphingolipid-metabolizing enzyme for growth of yeast Saccharomyces cerevisiae under impaired metabolism of glycerophospholipids., Mol. Microbiol., DOI: 10.1111/j.1365-2958.2010.07340.x, 78, 2, 395-413, 2010.08.
29. Nakase M, Tani M, Morita T, Kitamoto-K H, Kashiwazaki J, Nakamura T, Hosomi A, Tanaka N, Kaoru Takegawa, Mannosylinositol phosphorylceramide is a major sphingolipid component and is required for proper localization of plasma membrane proteins in Schizosaccharomyces pombe., J. Cell Sci., 123, 1578-1587, 2010.04.
30. Inoue T, Okino N, Kakuta Y, Hijikata A, Okano H, Goda H, Tani M, Sueyoshi N, Kambayashi K, Matsumura H, Kai H, Ito M, Mechanistic insights into the hydrolysis and synthesis of ceramide by neutral ceramidase., J. Biol. Chem., 284, 9566-9577, 2009.04.
31. Tani M, and Kuge O, Sphingomyelin synthase 2 is palmitoylated at the COOH-terminal tail, which is involved in its localization in plasma membranes., Biochem Biophys Res Commun., DOI: 10.1016/j.bbrc.2009.02.063, 381, 3, 328-332, 2009.02.
32. Hayashi Y, Okino N, Kakuta Y, Shikanai T, Tani M, Narimatsu H, Ito M, Klotho-related protein is a novel cytosolic neutral β–glycosylceramidase., J. Biol. Chem., 282, 30889-30900, 2007.10.
33. Ito K, Anada Y, Tani M, Ikeda M, Sano T, Kihara A, Igarashi Y, Lack of sphingosine 1-phosphate-degrading enzymes in erythrocytes., Biochem Biophys Res Commun., 357, 212-217, 2007.05.
34. Tani M, and Hannun YA., Analysis of membrane topology of neutral sphingomyelinase 2., FEBS lett., 581, 1323-1328, 2007.04.
35. Tani M, and Hannun YA., Neutral sphingomyelinase 2 is palmitoylated on multiple cysteine residues: Role of palmitoylation in subcellular localization. , J. Biol. Chem., 282, 13, 10047-10056, 2007.03.
36. Wu BX, Snook CF, Tani M, Büllesbach EE, and Hannun YA., Large-scale purification and characterization of recombinant Pseudomonas ceramidase: Regulation by calcium., J Lipid Res, 48, 600-608, 2006.08.
37. Tani M, Kihara A, and Igarashi Y., Rescue of cell growth by sphingosine with disruption of lipid microdomain formation of Saccharomyces cerevisiae deficient in sphingolipid biosynthesis., Biochem. J., 394, 1, 237-242, 2006.02.
38. Tani M, Igarashi Y, and Ito M., Involvement of neutral ceramidase in ceramide metabolism at the plasma membrane and in extracellular milieu., J. Biol. Chem., DOI: 10.1074/jbc.M506827200, 280, 44, 36592-36600, 2005.11.
39. Hwang Y, Tani M, Nakagawa T, Okino N, and Ito M., Subcellular localization of human neutral ceramidase expressed in HEK293 cells., Biochem Biophys Res Commun, 10.1016/j.bbrc.2005.03.134, 331, 1, 37-42, 2005.08.
40. Nakagawa T, Morotomi A, Tani M, Komori H, Sueyoshi N, and Ito M., C18:3-GM1a induces apoptosis in Neuro2a cells: enzymatic remodeling of fatty acyl chains of glycosphingolipids., J Lipid Res, 10.1194/jlr.M400516-JLR200, 46, 6, 1103-1112, 2005.08.
41. Tani M, Sano T, Ito M, and Igarashi Y., Mechanisms of sphingosine and sphingosine 1-phosphate generation in human platelets. , J. Lipid Res., 10.1194/jlr.M500268-JLR200, 46, 11, 2458-2467, 2005.08.
42. Yoshimura Y, Tani M, Okino N, Iida H, and Ito M., Molecular cloning and functional analysis of zebrafish neutral ceramidase., J Biol Chem, 10.1074/jbc.M405598200, 279, 42, 44012-44022, 2004.08.
43. Tani M, Okino N, Sueyoshi N, and Ito M., Conserved amino acid residues in the COOH-terminal tail are indispensable for the correct folding and localization, and enzyme activity of neutral ceramidase. , J. Biol. Chem., 10.1074/jbc.M404012200, 279, 28, 29351-29358, 2004.05.
44. Monjusho H, Okino N, Tani M, Maeda M, Yoshida M, and Ito M., A neutral ceramidase homologue of Dictyostelium discoideum exhibits an acidic pH optimum., Biochem J, 10.1042/BJ20030652, 376, 473-479, 2003.08.
45. Tani M, Iida H, and Ito M., O-glycosylation of mucin-like domain retains the neutral ceramidase on the plasma membranes as a type II integral membrane protein. , J. Biol. Chem., 10.1074/jbc.M207932200, 278, 12, 10523-10530, 2003.05.
46. Yoshimura Y, Okino N, Tani M, and Ito M., Molecular cloning and characterization of a secretory neutral ceramidase from Drosophila melanogaster., J Biochem, 132, 2, 229-236, 2002.08.
47. Mitsutake S, Tani M, Okino N, Mori K, Ichinose S, Omori A, Iida H, Nakamura T, and Ito M., Purification, characterization, molecular cloning, and subcellular distribution of neutral ceramidase of rat kidney., J Biol Chem, 10.1074/jbc.M102233200, 276, 28, 26249-26259, 2001.04.
48. Rommiti E, Meacci E, Tani M, Nuti F, Farnararo M, Ito M, and Bruni P., Neutral/alkaline and acid ceramidase activities are actively released by murine endothelial cells., Biochem Biophys Res Commun, 10.1006/bbrc.2000.3370, 275, 3, 746-751, 2000.09.
49. Tani M, Okino N, Mori K, Tanigawa T, Izu H, and Ito M., Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase., J Biol Chem , 10.1074/jbc.275.15.11229, 275, 15, 11229-11234, 2000.04.
50. Tani M, Okino N, Mitsutake S, and Ito M., Purification and characterization of a neutral ceramidase from mouse liver. , J Biol Chem , 10.1074/jbc.275.5.3462, 275, 5, 3462-3468, 2000.02.
51. Nakagawa T, Tani M, Kita K, and Ito M., Preparation of fluorescence-labeled GM1 and sphingomyelin by the reverse hydrolysis reaction of sphingolipid ceramide N-deacylase as substrates for assay of sphingolipid-degrading enzymes and for detection sphingolipid-binding proteins., J Biochem, 126, 126, 604-611, 1999.09.
52. Tani M, Okino N, Mitsutake S, and Ito M., Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide., J Biochem, 125, 4, 746-749, 1999.09.
53. Tani M, Kita K, Komori H, Nakagawa T, and Ito M., Enzymatic synthesis of omega-amino-ceramide: Preparation of a sensitive fluorescent substrate for ceramidase., Anal Biochem, 10.1006/abio.1998.2781, 263, 2, 183-188, 1998.10.
54. Okino N, Tani M, Imayama S, and Ito M., Purification and characterization of a novel ceramidase from Pseudomonas aeruginosa., J Biol Chem, 10.1074/jbc.273.23.14368, 273, 23, 14368-14373, 1998.06.