Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Daisuke Murakami Last modified date:2022.07.05

Lecturer / Otorhinolaryngology / Otorhinolaryngology Head and Neck Surgery / Kyushu University Hospital

1. Mukae N, Kuga D, Murakami D, Komune N, Miyamoto Y, Shimogawa T, Sakata A, Shigeto H, Iwaki T, Morioka T, Mizoguchi M. , Endonasal endoscopic surgery for temporal lobe epilepsy associated with sphenoidal encephalocele. , Surg Neurol Int. , 2021.07.
2. Sonoda Serika, Murakami Daisuke, Saito Yuichi, Miyamoto Yusuke, Higuchi Ryota, Kikuchi Yoshikazu, Sawatsubashi Motohiro, Nakagawa Takashi, Long-term effectiveness, safety, and quality of life outcomes following endoscopic posterior nasal neurectomy with submucosal turbinectomy for the treatment of intractable severe chronic rhinitis, AURIS NASUS LARYNX, 10.1016/j.anl.2020.12.009, 48, 4, 636-645, 2021.08.
3. Daisuke Murakami, Daisuke Kuga, Yusuke Miyamoto, Noritaka Komune, Nobutaka Mukae, Yuichi Saito, Koji Iihara, Takashi Nakagawa, A Pedicled Posterior Septal-Nasal Floor Flap and a Novel Rescue Flap for Skull Base Reconstruction., World Neurosurg., 2021.06.
4. Kikuchi Y, Umezaki T, Adachi K, Sawatsubashi M, Taura M, Yamaguchi Y, Tsuchihashia N, Murakami D, Nakagawa T, Awareness in young stuttering Japanese children aged 3-7 years. , Pediatr Int. , doi: 10.1111/ped.14405. , 2020.07.
5. Taisuke Narazaki, Yasuhiro Nakashima, Yasuhiro Tsukamoto, Ruriko Nishida, Mariko Tsuda, Hiroki Muta, Daisaku Kimura, Toru Masuda, Akiko Takamatsu, Kenichi Kohashi, Daisuke Murakami, Motoaki Shiratsuchi, Yoshihiro Ogawa, Schizophyllum commune sinusitis after allogeneic bone marrow transplantation for myelodysplastic syndrome
A case report and literature review, Transplant Infectious Disease, 10.1111/tid.13205, 22, 1, 2020.02, Sinusitis is a serious infectious complication of allogeneic hematopoietic stem cell transplantation. Schizophyllum commune (S commune) is a common basidiomycete fungus that is rarely involved in human disease. We report herein a case of S commune sinusitis after allogeneic bone marrow transplantation. A 66-year-old man with myelodysplastic syndrome underwent allogeneic bone marrow transplantation and developed maxillary and ethmoid sinusitis. The sinusitis did not improve with liposomal amphotericin B after neutrophil engraftment, so we considered that surgical intervention was needed for the recovery of sinusitis. Endoscopic sinus surgery was performed. In the debridement tissue of paranasal mucosa, filamentous fungal elements were observed. Moreover, genetic analysis of the tissue revealed the presence of S commune. Schizophyllum commune should be recognized as a fungal pathogen that causes sinusitis after allogeneic hematopoietic stem cell transplantation. This case suggests the effectiveness of prompt surgical intervention with liposomal amphotericin B treatment for S commune sinusitis and the usefulness of genetic diagnosis for cases under antifungal treatment. (160 words)..
6. Murakami D, Miyashita K, Koyama T, Omori H, Miyamoto Y, Sawatsubashi M, Nakagawa T, Clinicopathological analysis of sinonasal malignant lymphoma in the HTLV-1 endemic area, Japan–special interest in primary sinonasal diffuse large B-cell lymphoma–, J Clin Exp Hematop, 10.3960/jslrt.18008, 2019, 59, 101-107, 2019.09.
7. Hiroaki Saito, Yumi Sakakibara, Ayumi Sakata, Rie Kurashige, Daisuke Murakami, Hiroki Kageshima, Akira Saito, Yasunari Miyazaki, Antibacterial activity of lysozyme-chitosan oligosaccharide conjugates (LYZOX) against Pseudomonas aeruginosa, Acinetobacter baumannii and Methicillin-resistant Staphylococcus aureus, PloS one, 10.1371/journal.pone.0217504, 14, 5, 2019.05, The recent emergence of antibiotic-resistant bacteria requires the development of new antibiotics or new agents capable of enhancing antibiotic activity. This study evaluated the antibacterial activity of lysozyme-chitosan oligosaccharide conjugates (LYZOX) against Pseudomonas aeruginosa, Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus (MRSA), which should resolve the problem of antibiotic-resistant bacteria. Bactericidal tests showed that LYZOX killed 50% more P. aeruginosa (NBRC 13275), A. baumannii and MRSA than the control treatment after 60 min. In addition, LYZOX was shown to inhibit the growth of P. aeruginosa (NBRC 13275 and PAO1), A. baumannii and MRSA better than its components. To elucidate the antibacterial mechanism of LYZOX, we performed cell membrane integrity assays, N-phenyl-1-naphthylamine assays, 2-nitrophenyl β-D-galactopyranoside assays and confocal laser scanning microscopy. These results showed that LYZOX affected bacterial cell walls and increased the permeability of the outer membrane and the plasma membrane. Furthermore, each type of bacteria treated with LYZOX was observed by electron microscopy. Electron micrographs revealed that these bacteria had the morphological features of both lysozyme-treated and chitosan oligosaccharide- treated bacteria and that LYZOX destroyed bacterial cell walls, which caused the release of intracellular contents from cells. An acquired drug resistance test revealed that these bacteria were not able to acquire resistance to LYZOX. The hemolytic toxicity test demonstrated the low hemolytic activity of LYZOX. In conclusion, LYZOX exhibited antibacterial activity and low drug resistance in the presence of P. aeruginosa, A. baumannii and MRSA and showed low hemolytic toxicity. LYZOX affected bacterial membranes, leading to membrane disruption and the release of intracellular contents and consequent bacterial cell death. LYZOX may serve as a novel candidate drug that could be used for the control of refractory infections..
8. Daisuke Murakami, Futoshi Anan, Takayuki Masaki, Yoshikazu Umeno, Takehiko Shigenaga, Nobuoki Eshima, Takashi Nakagawa, Visceral fat accumulation is associated with asthma in patients with type 2 diabetes, Journal of diabetes research, 10.1155/2019/3129286, 2019, 2019.01, Objective. The number of patients with type 2 diabetes has increased in Japan, and type 2 diabetes has attracted attention as a risk factor for asthma. However, the risk factors for the development of asthma in patients with type 2 diabetes have not been determined. This study was performed to clarify whether visceral fat accumulation (VFA) and insulin resistance are risk factors for the development of asthma in patients with type 2 diabetes. Materials and Methods. A cross-sectional study was conducted. The study group comprised 15 patients with type 2 diabetes with asthma, and the control group comprised 145 patients with type 2 diabetes without asthma. Their fat distribution was evaluated by measuring the VFA by abdominal computed tomography at the umbilical level. Their glucose status was assessed by measuring the fasting plasma glucose (FPG) concentration, fasting immunoreactive insulin concentration, homeostasis model assessment (HOMA) index, and hemoglobin A1c concentration. Results. Among patients with type 2 diabetes, VFA was significantly greater in patients with asthma than those without asthma (P < 0 0001). The FPG concentration, fasting immunoreactive insulin concentration, and HOMA index were higher in patients with asthma than those without asthma (P < 0 05, P < 0 0001, and P < 0 0001, respectively). Multiple logistic regression analysis showed that VFA and the HOMA index were significantly associated with asthma in patients with type 2 diabetes (odds ratio, 1.78; 95% confidence interval, 1.31-3.89; P = 0 0115 and odds ratio, 3.65; 95% confidence interval, 1.37-7.85; P = 0 0078, respectively). Conclusions. Our data suggest that VFA and insulin resistance are associated with the development of asthma in patients with type 2 diabetes..
9. Yosikazu Kikuchi, Toshiro Umezaki, Taira Uehara, hiroo yamaguchi, Koji Yamashita, Hiwatashi Akio, Motohiro Sawatsubashi, Kazuo Adachi, Yumi Yamaguchi, Daisuke Murakami, Jun-Ichi Kira, Takashi Nakagawa, A case of multiple system atrophy-parkinsonian type with stuttering- and palilalia-like dysfluencies and putaminal atrophy, Journal of Fluency Disorders, 10.1016/j.jfludis.2017.11.002, 57, 51-58, 2018.09, Both developmental and acquired stuttering are related to the function of the basal ganglia-thalamocortical loop, which includes the putamen. Here, we present a case of stuttering- and palilalia-like dysfluencies that manifested as an early symptom of multiple system atrophy-parkinsonian type (MSA-P) and bilateral atrophy of the putamen. The patient was a 72-year-old man with no history of developmental stuttering who presented with a stutter for consultation with our otorhinolaryngology department. The patient was diagnosed with MSA-P based on parkinsonism, autonomic dysfunction, and bilateral putaminal atrophy revealed by T2-weighted magnetic resonance imaging. Treatment with levodopa improved both the motor functional deficits related to MSA-P and stuttering-like dysfluencies while reading; however, the palilalia-like dysfluencies were much less responsive to levodopa therapy. The patient died of aspiration pneumonia two years after his first consultation at our hospital. In conclusion, adult-onset stuttering- and palilalia-like dysfluencies warrant careful examination of the basal ganglia-thalamocortical loop, and especially the putamen, using neuroimaging techniques. Acquired stuttering may be related to deficits in dopaminergic function..
10. Daisuke Murakami, Sawatsubashi M, Takashi Nakagawa, Safety and efficacy of short-term oral immunotherapy with Cry j 1-galactomannan conjugate for Japanese cedar pollinosis: a randomized controlled trial, SCIENTIFIC REPORTS, 10.1038/srep46142, 7, 2017.04, Current allergen-specific immunotherapy (AIT) for pollinosis requires long-term treatment with potentially severe side effects. Therefore, development of an AIT that is safe and more convenient with a shorter regimen is needed. This prospective, double-blind, placebo-controlled trial randomized 55 participants with Japanese cedar pollinosis (JCP) to active or placebo groups to test the safety and efficacy of short-term oral immunotherapy (OIT) with Cry j 1-galactomannan conjugate for JCP. Mean symptom-medication score as the primary outcome in the active group improved 27.8% relative to the placebo group during the entire pollen season. As the secondary outcomes, mean medication score in active group improved significantly, by 56.2%, compared with placebo during the entire pollen season. Mean total symptom score was similar between active and placebo groups during the entire pollen season. There were no severe treatment-emergent adverse events in the active and placebo groups. Therefore short-term OIT with Cry j 1-galactomannan conjugate is safe, and effective for reducing the amount of medication use for JCP..
11. Daisuke Murakami, Topical treatment of eosinophilic otitis media and sinusitis using lysozyme-polysaccharide conjugates, Practica Oto-Rhino-Laryngologica, 10.5631/jibirin.109.822, 109, 12, 822-823, 2016.01, Eosinophilic otitis media and sinusitis are intractable disorders characterized by eosinophilic inflammation associated with bronchial asthma, which can be considered as a unified airway disease rather than as disorders with localized eosinophilic inflammation. The production of eosinophilic mucin and emergence of antibiotic-resistant bacteria at the site of inflammation are factors that make it difficult to treat eosinophilic otitis media and sinusitis. Therefore, development of a new topical agent with adequate antibacterial activity against the antibiotic-resistant bacteria and adequate efficacy for removing the viscous mucin is being sought. Therefore, we focused on lysozyme, which has already been used as a medical preparation, with its anti-inflammatory effects and antibacterial activity against some Gram-positive bacteria. In this study, we developed lysozyme-polysaccharide conjugates using the Maillard reaction to improve the aforementioned activities and examined the antibacterial activity against antibiotic-resistant bacteria and the inner ear toxicity of lysozyme-polysaccharide conjugates, for potential development of a cleaning agent for the middle ear. Testing of the antibacterial activity of lysozyme-chitosan conjugate showed antibacterial activities against MRSA and P. aeruginosa. In addition, the inner ear toxicity study in Guinea pigs revealed no obvious inner ear toxicity. Therefore lysozyme-chitosan conjugate are useful as a topical therapeutic agent (cleaning agent) and their clinical application is expected in the future..
12. Daisuke Murakami, Motohiro Sawatsubashi, Sayaka Kikkawa, Masayoshi Ejima, Akira Saito, Akio Kato, Shizuo Komune, Effect of short-term oral immunotherapy with Cry j 1-galactomannan conjugate on quality of life in Japanese cedar pollinosis patients
A prospective, randomized, open-label study, Journal of Otolaryngology of Japan, 10.3950/jibiinkoka.119.1263, 119, 9, 1263-1264, 2016.01.
13. Daisuke Murakami, Sawatsubashi M, Safety and efficacy of a new regimen of short-term oral immunotherapy with Cry j 1-galactomannan conjugate for Japanese cedar pollinosis: A prospective, randomized, open-label study, ALLERGOLOGY INTERNATIONAL, 10.1016/j.alit.2014.10.009, 64, 2, 161-168, 2015.04.
14. Motohiro Sawatsubashi, Daisuke Murakami, T. Umezaki, S. Komune, Endonasal endoscopic surgery with combined middle and inferior meatal antrostomies for fungal maxillary sinusitis, Journal of Laryngology and Otology, 10.1017/S0022215114002394, 129, S2, S52-S55, 2015.01, Objective: The purpose of this study was to evaluate the effectiveness of the combination of inferior and middle meatal antrostomies for treatment of a maxillary sinus fungus ball by functional endoscopic sinus surgery. Methods: A retrospective analysis including 28 patients with non-invasive fungal maxillary sinusitis was performed. Fourteen patients underwent FESS with both middle and inferior meatal antrostomies (combined group). The remaining 14 patients were treated with FESS through only the middle meatal antrostomy (control group). Results: Post-operative computed tomography showed normal maxillary sinuses in all patients in the combined group. In contrast, in the control group, five patients (36 per cent) exhibited a normal maxillary sinus, seven (50 per cent) showed maxillary mucosal thickening and two (14 per cent) had persistent fungus balls in the maxillary sinus. Conclusion: FESS with a combination of middle and inferior meatal antrostomies proved more effective for treating fungal maxillary sinusitis..
15. Motohiro Sawatsubashi, Daisuke Murakami, M. Oda, S. Komune, Transnasal endoscopic surgery of post-operative maxillary cysts, Journal of Laryngology and Otology, 10.1017/S0022215114002382, 129, S2, S46-S51, 2015.01, Objective: The present study investigates the indications for transnasal endoscopic surgery in treating post-operative maxillary cysts. Methods: In this retrospective study, the records of 118 patients with post-operative maxillary cysts (88 unilateral and 30 bilateral) consisting of 148 procedures were reviewed. Results: A transnasal endoscopic approach was performed in 144 lesions (97.3 per cent). A combined endonasal endoscopic and canine fossa (external) approach was performed in 4 of 148 lesions, because the cysts were located distant from the nasal cavity and had a thick bony wall. A ventilation stent was placed in four patients (four cysts) to avoid post-operative meatal antrostomy stenosis. Recurrence was observed in five patients (4.2 per cent), all of whom subsequently underwent transnasal endoscopic revision surgery. Conclusion: Transnasal endoscopic surgery is an effective treatment for post-operative maxillary cyst with the exception of cysts located distant from the nasal cavity..
16. Daisuke Murakami, Kazuhiko Kubo, Sawatsubashi M, Phase I/II study of oral immunotherapy with Cry j1-galactomannan conjugate for Japanese cedar pollinosis, AURIS NASUS LARYNX, 10.1016/j.anl.2014.02.010, 41, 4, 350-358, 2014.08.
17. Torahiko Nakashima, Atsuhiro Nakashima, Daisuke Murakami, Satoshi Toh, Hideki Shiratsuchi, Ryuji Yasumatsu, Ryuji Tominaga, Shizuo Komune, Follicular carcinoma of the thyroid with massive invasion into the cervical and mediastinum great veins
Our own experience and literature review, Laryngoscope, 10.1002/lary.23690, 122, 12, 2855-2857, 2012.12, Thyroid carcinomas with massive intralumen invasion of the great veins are extremely rare and reported to have poor prognosis. We report a case of a poorly differentiated follicular carcinoma of the thyroid with extensive invasion into the bilateral internal jugular veins, brachiocephalic vein, and superior vena cava. All of the seven major drainage veins from the thyroid were involved by tumor thrombus. The patient was successfully treated by surgical resection including removal of the tumor thrombus and repair of the great veins. The importance of preoperative radiological findings, treatment, and outcome are discussed along with a literature review..
18. Murakami D., Yamada H., Yajima T., Masuda A., Komune S. and Yoshikai Y., Lipopolysaccharide inhalation exacerbates allergic airway inflammation by activating mast cells and promoting Th2 responses., Clin. Exp.Allergy , 10.1111/j.1365-2222.2006.02633.x, 37, 3, 339-347, 2007.03.
19. Noriaki Aoi, Tokuko Masuda, Daisuke Murakami, Toshiki Yajima, Hiroyuki Mizubuchi, Hisakata Yamada, Hideyuki Kawauchi, Yasunobu Yoshikai, IL-15 prevents allergic rhinitis through reactivation of antigen-specific CD8 + cells, Journal of Allergy and Clinical Immunology, 10.1016/j.jaci.2006.02.018, 117, 6, 1359-1366, 2006.06, Background: Allergic rhinitis is one of the most common allergic inflammatory diseases characterized by a predominant T H2 response with antigen-specific IgE synthesis. IL-15 plays important roles in activation and maintenance of memory CD8 +T cells capable of producing IFN-γ, which regulates T H2 responses. Objective: To investigate the roles of endogenous IL-15 in allergic inflammation, we examined allergic rhinitis in IL-15 knockout (KO) mice sensitized with ovalbumin followed by intranasal rechallenge with ovalbumin. Methods: IL-15KO mice were sensitized intraperitoneally with ovalbumin/complete Freund's adjuvant on day 0 and ovalbumin/IFA on day 7, and then were intranasally challenged with ovalbumin on days 21, 22, 23, 24, and 25. Nasal symptoms and histologic changes were examined. IgE production and T H2 responses were measured by ELISA. Purified CD8 +T cells or recombinant IL-15 were administered into ovalbumin-sensitized mice. Results: The levels of IgE production and T H2 responses in IL-15KO mice were comparable to those in control mice after ovalbumin sensitization. However, sneezing, infiltration of eosinophils into the nasal mucosa, and T H2 cytokine production were aggravated in ovalbumin-sensitized IL-15KO mice after intranasal challenge with ovalbumin. Adoptive transfer of CD8 + T cells from ovalbumin-sensitized mice suppressed the T H2 responses in mice but not in IL-15KO mice. Administration of IL-15 with ovalbumin significantly prevented the development of allergic rhinitis in ovalbumin-sensitized mice. Conclusion: We demonstrate with IL-15KO mice that endogenous IL-15 plays an important role in suppression of allergic rhinitis at effector phase. Intranasal administration of IL-15 is useful as a therapeutic approach to control allergic rhinitis. Clinical implications: Intranasal administration of recombinant IL-15 might become new immunotherapy for allergic rhinitis..