九州大学 研究者情報
論文一覧
後藤 栄治(ごとうえいじ) データ更新日:2021.07.02

准教授 /  農学研究院 環境農学部門 森林環境科学


原著論文
1. Kazuhiro Ishishita, Takeshi Higa, Hidekazu Tanaka, Shin Ichiro Inoue, Aeri Chung, Tomokazu Ushijima, Tomonao Matsushita, Toshinori Kinoshita, Masato Nakai, Masamitsu Wada, Noriyuki Suetsugu, Eiji Gotoh, Phototropin2 contributes to the chloroplast avoidance response at the chloroplast-plasma membrane interface, Plant physiology, 10.1104/pp.20.00059, 183, 5, 304-316, 2020.05, [URL], Blue-light-induced chloroplast movements play an important role in maximizing light utilization for photosynthesis in plants. Under a weak light condition, chloroplasts accumulate to the cell surface to capture light efficiently (chloroplast accumulation response). Conversely, chloroplasts escape from strong light and move to the side wall to reduce photodamage (chloroplast avoidance response). The blue light receptor phototropin (phot) regulates these chloroplast movements and optimizes leaf photosynthesis by controlling other responses in addition to chloroplast movements. Seed plants such as Arabidopsis (Arabidopsis thaliana) have phot1 and phot2. They redundantly mediate phototropism, stomatal opening, leaf flattening, and the chloroplast accumulation response. However, the chloroplast avoidance response is induced by strong blue light and regulated primarily by phot2. Phots are localized mainly on the plasma membrane. However, a substantial amount of phot2 resides on the chloroplast outer envelope. Therefore, differentially localized phot2 might have different functions. To determine the functions of plasma membrane- and chloroplast envelope-localized phot2, we tethered it to these structures with their respective targeting signals. Plasma membrane-localized phot2 regulated phototropism, leaf flattening, stomatal opening, and chloroplast movements. Chloroplast envelope-localized phot2 failed to mediate phototropism, leaf flattening, and the chloroplast accumulation response but partially regulated the chloroplast avoidance response and stomatal opening. Based on the present and previous findings, we propose that phot2 localized at the interface between the plasma membrane and the chloroplasts is required for the chloroplast avoidance response and possibly for stomatal opening as well..
2. Eri Koide, Noriyuki Suetsugu, Megumi Iwano, Eiji Gotoh, Yuko Nomura, Sara Christina Stolze, Hirofumi Nakagami, Takayuki Kohchi, Ryuichi Nishihama, Regulation of Photosynthetic Carbohydrate Metabolism by a Raf-Like Kinase in the Liverwort Marchantia polymorpha, Plant & cell physiology, 10.1093/pcp/pcz232, 61, 3, 631-643, 2020.03, [URL], To optimize growth and development, plants monitor photosynthetic activities and appropriately regulate various cellular processes. However, signaling mechanisms that coordinate plant growth with photosynthesis remain poorly understood. To identify factors that are involved in signaling related to photosynthetic stimuli, we performed a phosphoproteomic analysis with Marchantia polymorpha, an extant bryophyte species in the basal lineage of land plants. Among proteins whose phosphorylation status changed differentially between dark-treated plants and those after light irradiation but failed to do so in the presence of a photosynthesis inhibitor, we identified a B4-group Raf-like kinase, named PHOTOSYNTHESIS-RELATED RAF (MpPRAF). Biochemical analyses confirmed photosynthesis-activity-dependent changes in the phosphorylation status of MpPRAF. Mutations in the MpPRAF gene resulted in growth retardation. Measurement of carbohydrates demonstrated both hyper-accumulation of starch and reduction of sucrose in Mppraf mutants. Neither inhibition of starch synthesis nor exogenous supply of sucrose alleviated the growth defect, suggesting serious impairment of Mppraf mutants in both the synthesis of sucrose and the repression of its catabolism. As a result of the compromised photosynthate metabolism, photosynthetic electron transport was downregulated in Mppraf mutants. A mutated MpPRAF with a common amino acid substitution for inactivating kinase activity was unable to rescue the Mppraf mutant defects. Our results provide evidence that MpPRAF is a photosynthesis signaling kinase that regulates sucrose metabolism..
3. Miki Kihara, Tomokazu Ushijima, Yoshiyuki Yamagata, Yukinari Tsuruda, Takeshi Higa, Tomomi Abiko, Takahiko Kubo, Masamitsu Wada, Noriyuki Suetsugu, Eiji Gotoh, Light-induced chloroplast movements in Oryza species, Journal of Plant Research, 10.1007/s10265-020-01189-w, 2020.01, [URL], Light-induced chloroplast movements control efficient light utilization in leaves, and thus, are essential for leaf photosynthesis and biomass production under fluctuating light conditions. Chloroplast movements have been intensively analyzed using wild-type and mutant plants of Arabidopsis thaliana. The molecular mechanism and the contribution to biomass production were elucidated. However, the knowledge of chloroplast movements is very scarce in other plant species, especially grass species including crop plants. Because chloroplast movements are efficient strategy to optimize light capture in leaves and thus promote leaf photosynthesis and biomass, analysis of chloroplast movements in crops is required for biomass production. Here, we analyzed chloroplast movements in a wide range of cultivated and wild species of genus Oryza. All examined Oryza species showed the blue-light-induced chloroplast movements. However, O. sativa and its ancestral species O. rufipogon, both of which are AA-genome species and usually grown in open condition where plants are exposed to full sunlight, showed the much weaker chloroplast movements than Oryza species that are usually grown under shade or semi-shade conditions, including O. officinalis, O. eichingeri, and O. granulata. Further detailed analyses of different O. officinalis accessions, including sun, semi-shade, and shade accessions, indicated that the difference in chloroplast movement strength between domesticated rice plants and wild species might result from the difference in habitat, and the shape of mesophyll chlorenchyma cells. The findings of this study provide useful information for optimizing Oryza growth conditions, and lay the groundwork for improving growth and yield in staple food crop Oryza sativa..
4. #張涵泳, 沖井英里香, 後藤栄治,宮原文彦,宮崎潤二,前田一,古澤英生, 宮里学,吉田茂二郎,白石進, 九州地域におけるマツノザイセンチュウ集団の遺伝的多様性と遺伝的構造, 日林誌, 101, 88-93, 2019.05.
5. Eiji Gotoh, Kohei Oiwamoto, Shin Ichiro Inoue, Ken Ichiro Shimazaki, Michio Doi, Stomatal response to blue light in crassulacean acid metabolism plants Kalanchoe pinnata and Kalanchoe daigremontiana, Journal of Experimental Botany, 10.1093/jxb/ery450, 70, 4, 1367-1374, 2019.02, [URL], Blue light (BL) is a fundamental cue for stomatal opening in both C 3 and C 4 plants. However, it is unknown whether crassulacean acid metabolism (CAM) plants open their stomata in response to BL. We investigated stomatal BL responses in the obligate CAM plants Kalanchoe pinnata and Kalanchoe daigremontiana that characteristically open their stomata at night and close them for part of the day, as contrasted with C 3 and C 4 plants. Stomata opened in response to weak BL superimposed on background red light in both intact leaves and detached epidermal peels of K. pinnata and K. daigremontiana. BL-dependent stomatal opening was completely inhibited by tautomycin and vanadate, which repress type 1 protein phosphatase and plasma membrane H + -ATPase, respectively. The plasma membrane H + -ATPase activator fusicoccin induced stomatal opening in the dark. Both BL and fusicoccin induced phosphorylation of the guard cell plasma membrane H + -ATPase in K. pinnata. These results indicate that BL-dependent stomatal opening occurs in the obligate CAM plants K. pinnata and K. daigremontiana independently of photosynthetic CO 2 assimilation mode..
6. Hanyong Zhang, Erika Okii, Eiji Gotoh, Fumihiko Miyahara, Junji Miyazaki, Hajime Maeda, Hideo Furusawa, Manabu Miyazato, Shigejiro Yoshida, Susumu Shiraishi, Genetic diversity and genetic structure of pine wood nematode (bursaphelenchus xylophilus) populations in the kyushu region, Nihon Ringakkai Shi/Journal of the Japanese Forestry Society, 10.4005/jjfs.101.88, 101, 2, 88-93, 2019.01, [URL], The genetic diversity and genetic structure of B. xylophilus in 8 populations of the Kyushu region were elucidated using the nucleotide polymorphism of 10 EST loci. Considerable genetic differences among regional populations were observed with the gene differentiation(GST) of 0.53, which showed that more than half of the total gene diversity(HT=0.63) was possessed among regional populations. The HT values of 8 regional populations were between 0.12 and 0.59. Sendai, Shintomi, Matsuura, and Karatsu populations were rich in gene diversity(0.59, 0.57, 0.56, and 0.55), and their high GST(0.43, 0.35, 0.25, and 0.25) indicated that the genetic compositions were notably different among the populations within damaged trees(subpopulations). On the other hand, in Amakusa and Miyazaki populations, extremely low gene diversity(0.12 and 0.18) and small GST(0.01 and 0.02) were confirmed, which showed that little genetic difference existed among subpopulations. It seemed that a bottleneck effect or founder effect had a great impact on the formation of these regional populations. The genetic diversity of the regional populations was polarized in Kyushu..
7. Eiji Gotoh, Noriyuki Suetsugu, Wataru Yamori, Kazuhiro Ishishita, Ryota Kiyabu, Masako Fukuda, Takeshi Higa, Bungo Shirouchi, Masamitsu Wada, Chloroplast Accumulation Response Enhances Leaf Photosynthesis and Plant Biomass Production, Plant Physiology, 10.1104/pp.18.00484, 178, 3, 1358-1369, 2018.11, [URL], Under high light intensity, chloroplasts avoid absorbing excess light by moving to anticlinal cell walls (avoidance response), but under low light intensity, chloroplasts accumulate along periclinal cell walls (accumulation response). In most plant species, these responses are induced by blue light and are mediated by the blue light photoreceptor, phototropin, which also regulates phototropism, leaf flattening, and stomatal opening. These phototropin-mediated responses could enhance photosynthesis and biomass production. Here, using various Arabidopsis (Arabidopsis thaliana) mutants deficient in chloroplast movement, we demonstrated that the accumulation response enhances leaf photosynthesis and plant biomass production. Conspicuously, phototropin2 mutant plants specifically defective in the avoidance response but not in other phototropin-mediated responses displayed a constitutive accumulation response irrespective of light intensities, enhanced leaf photosynthesis, and increased plant biomass production. Therefore, our findings provide clear experimental evidence of the importance of the chloroplast accumulation response in leaf photosynthesis and biomass production..
8. Gotoh Eiji, Suetsugu Noriyuki, Higa Takeshi, Matsushita Tomonao, Tsukaya Hirokazu, Wada Masamitsu, Palisade cell shape affects the light-induced chloroplast movements and leaf photosynthesis, SCIENTIFIC REPORTS, 10.1038/s41598-018-19896-9, 8, 2018.01, Leaf photosynthesis is regulated by multiple factors that help the plant to adapt to fluctuating light conditions. Leaves of sun-light-grown plants are thicker and contain more columnar palisade cells than those of shade-grown plants. Light-induced chloroplast movements are also essential for efficient leaf photosynthesis and facilitate efficient light utilization in leaf cells. Previous studies have demonstrated that leaves of most of the sun-grown plants exhibited no or very weak chloroplast movements and could accomplish efficient photosynthesis under strong light. To examine the relationship between palisade cell shape, chloroplast movement and distribution, and leaf photosynthesis, we used an Arabidopsis thaliana mutant, angustifolia (an), which has thick leaves that contain columnar palisade cells similar to those in the sun-grown plants. In the highly columnar cells of an mutant leaves, chloroplast movements were restricted. Nevertheless, under white light condition (at 120 µmol m−2 s−1), the an mutant plants showed higher chlorophyll content per unit leaf area and, thus, higher light absorption by the leaves than the wild type, which resulted in enhanced photosynthesis per unit leaf area. Our findings indicate that coordinated regulation of leaf cell shape and chloroplast movement according to the light conditions is pivotal for efficient leaf photosynthesis..
9. Hanyong Zhang, Erika Okii, Eiji Gotoh, Susumu Shiraishi, High mitochondrial genome diversity and intricate population structure of Bursaphelenchus xylophilus in Kyushu, Japan, Journal of Nematology, 10.21307/jofnem-2018-034, 50, 3, 281-302, 2018.01, [URL], Mitogenomic diversity and genetic population structure of the pinewood nematode (PWN) Bursaphelenchus xylophilus inhabiting Kyushu, Japan were analyzed. A method for performing long PCR using single nematodes and sequencing nematode mitochondrial genomes individually is presented here. About 8 kb (∼55%) of the complete mitochondrial genome was successfully obtained from 285 individuals collected from 12 populations. The 158 single nucleotide polymorphisms detected corresponded to 30 haplotypes, clearly classified into two clades. Haplotype diversity was 0.83, evidencing a remarkable high diversity within Kyushu. The high genetic differentiation among the 12 populations (0.331) might be due to past invasion and expansion routes of PWN in northeastern and southeastern Kyushu. The distinct genetic composition of populations within the northwestern, central western, and southwestern Kyushu seems to be mostly related to the extinction of pine forests and long-range migration of PWN due to human activity. Overall, direct long PCR and sequencing of single nematode individuals are effective methods for investigating mitochondrial polymorphisms, and these are effective tools for PWN population genetics and other intraspecific studies..
10. Tomokazu Ushijima, Kousuke Hanada, Eiji Gotoh, Wataru Yamori, Yutaka Kodama, Hiroyuki Tanaka, Miyako Kusano, Atsushi Fukushima, Mutsutomo Tokizawa, Yoshiharu Y. Yamamoto, Yasuomi Tada, Yutaka Suzuki, Tomonao Matsushita, Light Controls Protein Localization through Phytochrome-Mediated Alternative Promoter Selection, Cell, 10.1016/j.cell.2017.10.018, 171, 6, 1316-1325.e12, 2017.11, [URL], Alternative promoter usage is a proteome-expanding mechanism that allows multiple pre-mRNAs to be transcribed from a single gene. The impact of this mechanism on the proteome and whether it is positively exploited in normal organismal responses remain unclear. We found that the plant photoreceptor phytochrome induces genome-wide changes in alternative promoter selection in Arabidopsis thaliana. Through this mechanism, protein isoforms with different N termini are produced that display light-dependent differences in localization. For instance, shade-grown plants accumulate a cytoplasmic isoform of glycerate kinase (GLYK), an essential photorespiration enzyme that was previously thought to localize exclusively to the chloroplast. Cytoplasmic GLYK constitutes a photorespiratory bypass that alleviates fluctuating light-induced photoinhibition. Therefore, phytochrome controls alternative promoter selection to modulate protein localization in response to changing light conditions. This study suggests that alternative promoter usage represents another ubiquitous layer of gene expression regulation in eukaryotes that contributes to diversification of the proteome..
11. Shin-ichiro Inoue, Nozomi Iwashita, Yohei Takahashi, Eiji Gotoh, Eiji Okuma, Maki Hayashi, Ryohei Tabata, Atsushi Takemiya, Yoshiyuki Murata, Michio Doi, Toshinori Kinoshita, 島崎 研一郎, Brassinosteroid Involvement in Arabidopsis thaliana Stomatal Opening, PLANT AND CELL PHYSIOLOGY, 10.1093/pcp/pcx049, 58, 6, 1048-1058, 2017.06.
12. Morihiro Oota, Eiji Gotoh, Masaharu Endo, Takashi Ishida, Tomonao Matsushita, Shinichiro Sawa, Negative phototaxis in M. incognita, International Journal of Biology, 9, 3, 2017.05.
13. Noriyuki Suetsugu*, Takeshi Higa*, Eiji Gotoh*, Masamitsu, Light-Induced Movements of Chloroplasts and Nuclei Are Regulated in Both Cp-Actin-Filament-Dependent and -Independent Manners in Arabidopsis thaliana, PLOS ONE, 10.1371/journal.pone.0157429, 11, 6, *These authors contributed equally., 2016.06.
14. Ishishita Kazuhiro, Noriyuki Suetsugu, Yuki Hirose, Tomonao Matsushita, Takeshi Higa, Masamitsu Wada, Michio Doi, Eiji Gotoh, Functional characterization of blue-light-induced responses and PHOTOTROPIN 1 gene in Welwitschia mirabilis, JOURNAL OF PLANT RESEARCH, 10.1007/s10265-016-0790-7, 129, 2, 175-187, 2016.03.
15. D. V. Thao, A. Y P B C Widyatmoko, L. Guan, Eiji Gotoh, Atsushi Watanabe, S. Shiraishi, Isolation and characterization of tetranucleotide microsatellite markers for Pinus merkusii, Conservation Genetics Resources, 10.1007/s12686-012-9821-4, 5, 2, 433-436, 2013.05, [URL].
16. Vivi Yuskianti, Lanhua Guan, Eiji Gotoh, Anthonius Y.P.B.C. Widyatmoko, Susumu Shiraishi, Development of tetranucleotide microsatellite markers in Falcataria moluccana, Nihon Ringakkai Shi/Journal of the Japanese Forestry Society, 94, 2, 92-94, 2012.06.
17. Eiji Gotoh, Yoshichika Kobayashi, Michito Tsuyama, The post-illumination chlorophyll fluorescence transient indicates the RuBP regeneration limitation of photosynthesis in low light in Arabidopsis, FEBS Letters, 10.1016/j.febslet.2010.05.039, 584, 14, 3061-3064, 2010.07, [URL], The mechanism of post-illumination chlorophyll fluorescence transient (PIFT) was investigated in Arabidopsis. PIFT was detected in the wild type after illumination with low light. In the fba3-2 (fructose-1,6-bisphosphate aldolase) mutant, in which PIFT is enhanced, strong light also induced PIFT. PIFT was suppressed not only in the triose phosphate/phosphate translocator (tpt-2) mutant, but also in tpt-2 fba3-2, suggesting that triose phosphates, such as dihydroxyacetone phosphate (DHAP), are involved in the PIFT mechanism. We concluded that PIFT is associated with ribulose-1,5-bisphosphate (RuBP)-regeneration limitation of photosynthesis in low light..
18. Eiji Gotoh, Masayoshi Matsumoto, Ken'ichi Ogawa, Yoshichika Kobayashi, Michito Tsuyama, A qualitative analysis of the regulation of cyclic electron flow around photosystem I from the post-illumination chlorophyll fluorescence transient in Arabidopsis
A new platform for the in vivo investigation of the chloroplast redox state, Photosynthesis Research, 10.1007/s11120-009-9525-0, 103, 2, 111-123, 2010.01, [URL], A transient in chlorophyll fluorescence after cessation of actinic light illumination, which has been ascribed to electron donation from stromal reductants to plastoquinone (PQ) by the NAD(P)H-dehydrogenase (NDH) complex, was investigated in Arabidopsis thaliana. The transient was absent in air in a mutant lacking the NDH complex (ndhM). However, in ndhM, the transient was detected in CO2-free air containing 2% O2. To investigate the reason, ndhM was crossed with a pgr5 mutant impaired in ferredoxin (Fd)-dependent electron donation from NADPH to PQ, which is known to be redundant for NDH-dependent PQ reduction in the cyclic electron flow around photosystem I (PSI). In ndhM pgr5, the transient was absent even in CO2-free air with 2% O2, demonstrating that the post-illumination transient can also be induced by the Fd- (or PGR5)-dependent PQ reduction. On the other hand, the transient increase in chlorophyll fluorescence was found to be enhanced in normal air in a mutant impaired in plastid fructose-1,6-bisphosphate aldolase (FBA) activity. The mutant, termed fba3-1, offers unique opportunities to examine the relative contribution of the two paths, i.e., the NDH- and Fd- (or PGR5)-dependent paths, on the PSI cyclic electron flow. Crossing fba3-1 with either ndhM or pgr5 and assessing the transient suggested that the main route for the PSI cyclic electron flow shifts from the NDH-dependent path to the Fd-dependent path in response to sink limitation of linear electron flow..
19. Kensuke Kusumi, Yoko Chono, Hiroshi Shimada, Eiji Gotoh, Michito Tsuyama, Koh Iba, Chloroplast biogenesis during the early stage of leaf development in rice, Plant Biotechnology, 27, 1, 85-90, 2010, In rice, the developmental process in leaf formation can be divided into 7 stages (stages P0 to P6). We investigated chloroplast biogenesis and physiological changes in the developing leaves at the stage P4, during which leaf blade elongation and establishment of basic leaf blade structure occur. Chlorophyll content was negligible in the leaves early in the P4 stage and increased rapidly as they enters the late P4 phase. Chlorophyll fluorescence ratio (Fv/Fm) also increased markedly and the final value was comparable with that of mature leaves. Gene expression analysis showed that during the P4 stage, chloroplasts in the leaf cell undergo all three steps of differentiation: (i) plastid division and DNA replication (ii) establishment of plastid genetic system (iii) activation of photosynthetic apparatus. These observations suggest that the P4 is key in the development of a leaf, during which leaf rapidly differentiated both morphologically and physiologically, and that the P4 leaf is suitable for investigation of physiological relationships between chloroplast and leaf development..

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