Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Takahashi Hideyuki Last modified date:2021.08.27

Associate Professor / Animal and Marine Bioresource Sciences / University Farm,Kyushu University / Faculty of Agriculture

1. Yutaka Taguchi, Yudai Inabu, Koki Hayasaki, Noriyuki Maeda, Yoshiro Kanmera, Seiji Yamasaki, Noboru Ota, Kenji Mukawa, Arisa Tsuboi, Hirokuni Miyamoto, Tetsuji Etoh, Yuji Shiotsuka, Ryoichi Fujino, Christopher D. McMahon, Hideyuki Takahashi, Effects of feeding high volumes of milk replacer on reproductive performance and on concentrations of metabolites and hormones in blood of Japanese black heifer calves, Animal Science Journal,, 2021.01, 肉牛生産現場では仔牛の減少に伴う仔牛取引価格の高騰が問題になっている。この問題を解決するためには母牛の生涯産仔数を増やすことが必須である。そのためには早期に人工授精(AI:Artificial insemination)が可能な体格を獲得させて初産分娩月齢を早める必要がある。これまでの研究成果より哺乳期の代用乳多給がメス仔牛の発育(特に体高)を向上させることが明らかとなり、代用乳多給によって初産分娩月齢を早期化できる可能性が考えられた。しかし、代用乳多給は標準的なプランが確立されておらず、増体および繁殖成績に及ぼす影響は不明である。
2. Al Nur Md Iftekhar Rahman, Seiya Yamashita, Md Rashedul Islam, Taisuke Fujihara, Hayato Yamaguchi, Manabu Kawahara, Masashi Takahashi, Hideyuki Takahashi, Takafumi Gotoh, Nobuhiko Yamauchi, Type-I interferon regulates matrix metalloproteinases clearance of the bovine endometrial spheroid, Animal Science Journal, 10.1111/asj.13350, 91, 1, 2020.01, This study investigated the effect of type-I interferon (IFN) on the expression of matrix metalloproteinases (MMPs) of the bovine endometrial stromal cells (BES) and epithelial cells (BEE). The cells were separated and purified from the caruncles and cultured in DMEM/F-12 containing 10% fetal bovine serum. Spheroids were generated by using ascorbate. Zymograms of the supernatant showed that BEE predominantly expressed MMP-9, whereas MMP-2 was expressed in BES and homo-spheroids. While MMPs expression was not detected in hetero-spheroids. Real-time quantitative PCR revealed that type-I IFN and P4 suppressed the gene expression of MMP-2 and MMP-9 in hetero-spheroids, respectively. On the other hand, gelatin zymography analysis of the supernatant showed that type-I IFN strongly promote the clearance of MMPs. While zymograms of the MMPs stocked in the hetero-spheroids were significantly reduced by type-I IFN. Phenylmethanesulfonyl fluoride and leupeptin (both are serine proteinase inhibitors) significantly repressed the clearance of MMP-2 and MMP-9 induced by type-I IFN. Moreover, collagen fibers in hetero-spheroids significantly decreased after the treatment with type-I IFN. In conclusion, it was suggested that type-I IFN participate in the tissue remodeling by regulation the clearance of MMPs..
3. Katsuhiko Tsuruoka,Takami Kurahara,Hidenobu Kanamaru,Hideyuki Takahashi,Takafumi Gotoh., Effect of feeding rice whole crop silage on growth rate, levels of vitamin A, β‐carotene, vitamin E and IGF‐1 in plasma and skeletal muscle protein degradation in Japanese black calves., Animal Science Journal, 2019.04, This study evaluated the effects of rice whole crop silage (RWCS) on growth, plasma levels of vitamin A, β‐carotene, vitamin E and IGF‐1, and expression of genes involved in muscle protein degradation and synthesis in Japanese Black calves. Eleven calves were divided into RWCS (fed RWCS ad libitum and concentrate, n = 5) and control groups (fed hay ad libitum and concentrate, n = 6). Final body weight and dairy gain were significantly larger in the RWCS group compared with the control group. Plasma β‐carotene and vitamin E concentrations were significantly higher in the RWCS group compared with control group. Although plasma vitamin E concentration in the RWCS group significantly increased from 4 to 9 months of age, it did not increase in the control group. At 6 months of age in the RWCS group, ubiquitin B (p < 0.05) and calpain 1 (p = 0.097) mRNA expression were lower than control group, but they were not different between groups at 9 months of age. These results indicate that RWCS increases plasma β‐carotene level and promotes muscle growth because of a decrease in the rate of protein degradation, but the effect is lost with the increase in plasma vitamin E level..
4. Katsuhiko Tsuruoka, Takami Kurahara, Hidenobu Kanamaru, Hideyuki Takahashi, Takafumi Gotoh., Effects of feeding condensed barley distillers soluble on growth rate, ruminal fermentation, plasma metabolites, and myofiber properties of the longissimus thoracis muscle in Japanese Black calves., Animal Science Journal,, 90, 5, 2019.05, This study evaluated the effects of condensed barley distillers soluble (CBDS) on growth rate, rumen fermentation, plasma metabolite, and myofiber properties, and gene expression related to metabolism in the skeletal muscles of Japanese Black calves, compared with soybean meal and corn. Twenty‐four calves were divided into four groups: fed 5% CBDS based on the hay dry matter weight (low CBDS) and fed soybean meal and corn at the same nutrition level (control); and fed 15% CBDS based on the hay dry matter weight (high CBDS) and fed soybean meal and corn at the same nutrition level (high soy). The daily gain was larger in the low (p = 0.08) and high (p < 0.05) CBDS groups compared with the control group. In the CBDS‐fed groups, plasma β‐hydroxybutyric acid concentrations were significantly higher at 6 months of age (p < 0.05), the percentage of type I myofibers was significantly lower and their diameters were significantly larger at 9 months of age (p < 0.05), and carnitine palmitoyltransferase 1b mRNA expression was significantly lower (p < 0.05) and citrate synthase mRNA expression tended to be lower (low; p = 0.06, high; p = 0.05) compared with control group. Thus, feeding CBDS promotes growth and leads to animals with more glycolytic and less oxidative muscle metabolism..
5. Toru Asahina, Kosuke Nakagiri, Yuji Shiotsuka, Tetsuji Etoh, Ryoichi Fujino, Nonomi Suzuki, Christopher D. McMahon, Hideyuki Takahashi, Physiological Responses of Japanese Black Calves to Supplementation with Sodium Butyrate in Milk Replacer, Food & Nutrition Journal, 10.29011/2575-7091. 100083, 2018.06, Genetic factors are important for meat quality in cattle and gene expression may be determined by epigenetic control. This may involve Histone Deacetylases (HDACs), which suppress or enhance gene expression, and beta-hydroxybutyric acid (BHBA, produced from butyric acid), which inhibits HDAC activity and increases gene expression. This study aimed to determine how supplementing Japanese Black calves with sodium butyrate in milk replacer affects the relationship between plasma BHBA, HDAC activity in tissues, and blood metabolites. Eight Japanese Black female calves were randomly assigned to two groups of four animals each (control group, and supplementation with sodium butyrate group; NaB). Calves in both groups were fed milk replacer containing 26% Crude Protein (CP), 25.5% Crude Fat (CF), and 116% Total Digestible Nutrients (TDNs), and all calves received calf starter and hay ad libitum. Sodium butyrate was administered to the calves in the NaB group at daily doses of 3 g (from 3 to 30 days of age), 5 g (from 31 to 60 days of age), and 7 g (from 61 to 90 days of age). The supplementation of Japanese Black calves with sodium butyrate in milk replacer decreased plasma concentrations of BHBA..
6. H Takahashi, Y Suzuki, JS Mohamed, T Gotoh, SL Pereira, SE Alway, Epigallocatechin-3-gallate increases autophagy signaling in resting and unloaded plantaris muscles but selectively suppresses autophagy protein abundance in reloaded muscles of aged rats, Experimental Gerontology,, 92, 56-66, 2017.06, We have previously found that Epigallocatechin-3-gallate (EGCg), an abundant catechin in green tea, reduced apoptotic signaling and improved muscle recovery in response to reloading after hindlimb suspension (HS). In this study, we investigated if EGCg altered autophagy signaling in skeletal muscle of old rats in response to HS or reloading after HS. Fischer 344 x Brown Norway inbred rats (age 34 months) were given 1 ml/day of purified EGCg (50 mg/kg body weight), or the same sample volume of the vehicle by gavage. One group of animals received HS for 14 days and the second group of rats received 14 days of HS, then the HS was removed and they were allowed to recover by ambulating normally around the cage for two weeks. EGCg decreased a small number of autophagy genes in control muscles, but it increased the expression of other autophagy genes (e.g., ATG16L2, SNCA, TM9SF1, Pink1, PIM-2) and HS did not attenuate these increases. HS increased Beclinl, ATG7 and LC3-II/I protein abundance in hindlimb muscles. Relative to vehicle treatment, EGCg treatment had greater ATG12 protein abundance (35.8%, P < 0.05), but decreased Beclinl protein levels (-101.1%, P < 0.05) after HS. However, in reloaded muscles, EGCg suppressed Beclinl and LC3-II/I protein abundance as compared to vehicle treated muscles. EGCg appeared to "prime" autophagy signaling before and enhance autophagy gene expression and protein levels during unloading in muscles of aged rats, perhaps to improve the clearance of damaged organelles. However, EGCg suppressed autophagy signaling after reloading, potentially to increase the recovery of hindlimb muscles mass and function after loading is restored. (C) 2017 Elsevier Inc. All rights reserved..
7. P Ouanh, @H Takahashi, H Ma, Y Shiotsuka, A Matsubara, T Sugino, C McMahon, T Etoh, R Fujino, M Furuse, T Gotoh, Effects of nutritional status on hormone concentrations of the somatotropin axis and metabolites in plasma and colostrum of Japanese Black cows, Animal Science Journal, 10.1111/asj.12686, 88, 4, 643-652, 2017.04, We aimed to determine the effects of nutritional status on concentrations of somatotropic axis hormones (growth hormone (GH) and insulin-like growth factor 1 (IGF-1)), insulin and metabolites (glucose, total protein and nonesterified fatty acids (NEFA)) in the plasma and colostrum in late antepartum cows. Eight pregnant Japanese Black cows were randomly assigned to two experimental groups (n=4 per group). Control cows (CON) received 100% of their nutritional requirements until parturition, whereas restricted group cows (RES) received 60% of their nutritional requirements. Blood samples were taken during the antepartum period, and blood and colostrum samples were collected on days 0, 1, and 3 after calving. Compared to the CON group, the RES group had higher concentrations of GH and NEFA in plasma, but significantly lower concentrations of glucose and insulin in plasma. The concentrations of GH in plasma after calving were significantly higher, but total plasma protein was significantly lower in RES than in CON cows. Compared to the CON group, the RES group had significantly higher concentrations of GH in colostrum, but significantly lower total concentrations of protein in colostrum. Concentrations of IGF-1 were not different between the two groups. These findings suggest that maternal nutritional status during late gestation influences concentrations of GH and total protein in the blood and colostrum of Japanese Black cows.
8. Hideyuki Takahashi, Atsuko Matsubara, Akira Saito, Ouanh Phomvisith, Akari Shiga, Ha T Mai, Toshihisa Sugino, Christopher D McMahon, Tetsuji Etoh, Yuji Shiotsuka, Ryoichi Fujino, M Furuse, T Gotoh, Higher intake of milk-replacer pre-weaning enhances post-weaning insulin-like growth factor 1 levels in Japanese Black cattle, Journal of Veterinary Science & Technology, 10.4172/2157-7579.1000409, 8, 1, 409, 2017.01, Alterations in early pre-natal nutrition of Japanese Black calves influence the glucose and lipid metabolism after weaning. However, the effects of early nutritional status on the endocrine system in later life stages in Japanese Black cattle are unknown. This study examined how post-weaning plasma levels of growth hormone (GH), insulin-like growth factor 1 (IGF-1), and blood insulin, which are hormones affecting growth and meat quality, and metabolites were affected by feeding 1800 g versus 500 g of milk replacer to Japanese Black cattle (5 per group) during nursing. Up to weaning (90 days post-birth), all calves received calf starter and hay ad libitum, and post-weaning, they received a concentrate feed and hay ad libitum. Plasma concentrations of GH and IGF-1 were greater at weaning in the high-milk-replacer group (P<0.1 and P<0.01, respectively), and elevated IGF-1 concentrations persisted until the study end (210 d) (P<0.05), suggesting that the levels were sustained independent of the influences of both GH and nutrient intake. Blood insulin and metabolites (plasma glucose, beta hydroxybutyric acid, and non-esterified fatty acids) were not significantly different between the two groups. The results of this study suggest that feeding calves a high volume of milk replacer during nursing will increase IGF-1 secretion well beyond weaning..
9. A Matsubara, H Takahashi, A Saito, A Nomura, K Sithyphone, CD McMahon, R Fujino, Y Shiotsuka, T Etoh, M Furuse, T Gotoh, Effects of a high milk intake during the pre-weaning period on nutrient metabolism and growth rate in Japanese Black cattle, Animal Science Journal, 10.1111/asj.12547, 87, 9, 1130-1136, 2016.09, This study aimed to determine the effects of feeding an increased volume of high-fat milk during the early post-natal life on metabolite concentrations in the blood, the expression of key genes regulating intermediary metabolism in the skeletal muscles, and the rate of growth of Japanese Black cattle. All calves were fed a high-fat milk replacer (crude protein, 26%; crude fat, 25.5%; total dissolved nitrogen, 116%). Control calves (n = 4) were nursed with 500 g milk replacer until 3 months of age (mo), whereas calves in the experimental group (n = 4) were nursed with 1,800 g milk replacer until 3 mo, and then the volume was gradually reduced until 5 mo. Body weight was significantly higher in the experimental group than in the control group at 7 mo. Plasma glucose concentrations were significantly lower in the experimental group. Expression of glucose-transporter-4 mRNA was lower, whereas that of glucose transporter 1, cluster of differentiation 36, and carnitine palmitoyltransferase-1b mRNA was significantly higher in the M. longissimus thoracis of the experimental group. Nutritional status during early post-natal life appears to strongly influence the growth rate and glucose and lipid metabolism in Japanese Black cattle..
10. K Tsuruoka, H Kanamaru, H Takahashi, Takaufmi Gotoh, Preliminary study of the effects of condensed barley distillers soluble on rumen fermentation and plasma metabolites in Japanese Black cows , Animal Science Journal, 2016.06.
11. Tatsunori Masatani, Shunpei Yoshihara, Atsuko Matsubara, Takaufmi Gotoh, H Takahashi, Tetsuya Tanaka, Masako Andoh, Yasuyuki Endo, Tomohide Matsuo, Dynamics of Theileria orientalis genotype population in cattle in a year-round grazing system, Acta Parasitologica, 10.1515/ap-2016-0056, 61 , 419-424, 2016.03.
12. T Gotoh, H Takahashi, T Nishimura, K Kuchida, H Mannen, Meat Produced by Japanese Black Cattle and Wagyu, Animal Frontiers, 2014.10.
13. H Takahashi, K Sato, T Yamaguchi, M Miyake, H Watanabe, Y Nagasawa, E Kitagawa, S Terada, M Urakawa, M. T. Rose, C. D. MacMahon, K Watanabe, S Ohwada, T Gotoh, H Aso, Myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double-muscled (DM) and normal-muscled (NM) Japanese shorthorn cattle, Domestic Animal Endocrinology, 48, 62-68, 2014.02, ミオスタチンのエネルギー代謝に及ぼす影響を明らかにするため、ミオスタチンを欠損した草原短角牛ならびにミオスタチンが発現している日本短角種牛の骨格筋を用いて、グルコース輸送担体であるGLUT1、GLUT4のmRNA発現、グルコース取り込みに関与するインスリン受容体mRNA発現ならびに脂肪酸輸送担体であるCD36、CPT-1 mRNA発現をリアルタイムPCR法により解析をした。その結果、ミオスタチン欠損した草原短角牛の骨格筋では、インスリン依存型グルコース輸送担体であるGLUT4mRNA発現が上昇していることが明らかとなった。また、脂質代謝については、血中の遊離脂肪酸濃度が草原短角牛で低いことが明らかとなった。
 次に、草原短角牛ならびに日本短角種牛骨格筋筋芽細胞を用いて、筋管への分化誘導後0時、24時ならびに72時間後に同様の遺伝子発現を解析した。その結果、草原短角牛筋芽細胞では、筋分化後72時間においてGLUT4mRNA発現が上昇することが明らかとなり、 一方で、インスリン受容体、GLUT1mRNA発現は両種の間に差はなかった。 以上のことより、ミオスタチンはGLUT4発現を介してグルコース代謝を亢進することが考えられ、さらにその作用はインスリン感受性を上昇させることにより行われる可能性が示唆された。.
14. Hitoshi Watanabe, Ryo Saito, Tatsuya Nakano, H Takahashi, Yu Takahashi, Keisuke Sumiyoshi, Katsuyoshi Sato, Xiangning Chen, Natsumi Okada, Shunsuke Iwasaki, Dian W. Harjanti, Natsumi Sekiguchi, Hiroaki Sano, Haruki Kitazawa, Michael T. Rose, Shyuichi Ohwada, Kouichi Watanabe, Hisashi Aso, Effect of Peripheral 5-HT on Glucose and Lipid Metabolism in Wether Sheep., PLOS ONE, 10.1371, 9, 2, e88058, 2014.02.
15. Ogino M, Matsuura A, Yamazaki A, Irimajiri A, Takahashi Hideyuki, Komatsu T, Kushibiki S, Shingu H, Kasuya E, Hasegawa Y, Hodate K, Biological rhythms related to metabolism in Japanese Shorthorn cattle under varying environments and management techniques, Animal Science Journal, 10.1111, 2013.01.
16. Masato Miyake., Hideyuki Takahashi., Eri Kitagawa., Hitoshi Watanabe., Takahiro Sakurada., Hisashi Aso and Takahiro Yamaguchi. , AMPK activation by AICAR inhibits myogenic differentiation and myostatin expression in Cattle. , CELL & TISSUE RESEARCH, 2012.04.
17. Suzuki Y., Kurose Y., Takahashi H., Asakuma S., Azuma Y and Kobayashi S., The differences in feeding-inhibitory responses to peripheral and central leptin between non-lactating and lactating rats., Journal of Endocrinology, 2010.11.
18. Takahashi H., Kurose Y., Suzuki Y., Kojima M., Yamaguchi T., Yoshida Y., Azuma Y., Sugino T., Kojima M., Kangawa K., Hasegawa Y and Kobayashi S., Changes in blood pancreatic polypeptide and ghrelin levels in response to feeding in sheep., Journal of Animal Science, 2010.06.
19. Miyake M., Hayashi S., Iwasaki S., Chao G., Takahashi H., Watanabe K., Ohwada S., Aso H., Yamaguchi T., Possible role of TIEG1 as a feedback regulator of myostatin and TGF-beta in myoblasts. , Biochemical and Biophysical Research Communications., 2010.03.
20. Takahashi H., Kurose Y., Suzuki Y., Kojima M., Yamaguchi T., Yoshida Y., Ogino M., Hodate K., Azuma Y., Sugino T., Kojima M., Kangawa K., Hasegawa Y and Kobayashi S., Ghrelin differentially modulates the GH secretory response to GHRH between the fed and fasted state in sheep., Domestic Animal Endocrinology, 2009.12.
21. Takahashi H., Kurose Y., Sakaida M., Suzuki Y., Kobayashi S., Sugino T., Kojima M., Kangawa K., Hasegawa Y and Terashima Y., Ghrelin differentially modulates glucose-induced insulin secretion according to feeding status in sheep., Journal of Endocrinology, 2007.09.
22. Takahashi H., Kurose Y., Kobayashi S., Sugino T., Kojima M., Kangawa K., Hasegawa Y and Terashima Y., Ghrelin enhances glucose-induced insulin secretion in scheduled meal-fed sheep., Journal of Endocrinology, 2006.04.
23. Youhei Kurose, Atuko Kuwahara, Yuuki Oshiba, Hideyuki Takahashi, Yuki Watanabe, Yoshiaki Terashima, Effect of intravenous administration of glucagon-like peptide 1 on growth hormone secretion in sheep, Journal of Animal and Feed Science, 10.22358/jafs/73958/2004, 13, 1, 433-435, 2004.05.