Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Takashi Miura Last modified date:2020.07.17

Professor / Department of Anatomy and Cell Biology / Department of Basic Medicine / Faculty of Medical Sciences


Papers
1. Satoru Okuda, Takashi Miura, Yasuhiro Inoue, Taiji Adachi, Mototsugu Eiraku, Publisher Correction
Combining Turing and 3D vertex models reproduces autonomous multicellular morphogenesis with undulation, tubulation, and branching (Scientific Reports (2018) DOI: 10.1038/s41598-018-20678-6), Scientific reports, 10.1038/s41598-018-24858-2, 8, 1, 2018.12, This Article contains errors in the order of the Figures and Figure Legends. In the HTML version of the Article, Figures 8 and 9 were published in the reverse order, but not their accompanying legends. In the PDF version of the Article, the legends of Figures 8 and 9 only were published in the reverse order. The correct Figures 8 and 9 together with their accompanying legends appear below as Figures 1 and 2 respectively. (Figure Presented).
2. Satoru Okuda, Takashi Miura, Yasuhiro Inoue, Taiji Adachi, Mototsugu Eiraku, Combining Turing and 3D vertex models reproduces autonomous multicellular morphogenesis with undulation, tubulation, and branching, Scientific reports, 10.1038/s41598-018-20678-6, 8, 1, 2018.12, This study demonstrates computational simulations of multicellular deformation coupled with chemical patterning in the three-dimensional (3D) space. To address these aspects, we proposes a novel mathematical model, where a reaction-diffusion system is discretely expressed at a single cell level and combined with a 3D vertex model. To investigate complex phenomena emerging from the coupling of patterning and deformation, as an example, we employed an activator-inhibitor system and converted the activator concentration of individual cells into their growth rate. Despite the simplicity of the model, by growing a monolayer cell vesicle, the coupling system provided rich morphological dynamics such as undulation, tubulation, and branching. Interestingly, the morphological variety depends on the difference in time scales between patterning and deformation, and can be partially understood by the intrinsic hysteresis in the activator-inhibitor system with domain growth. Importantly, the model can be applied to 3D multicellular dynamics that couple the reaction-diffusion patterning with various cell behaviors, such as deformation, rearrangement, division, apoptosis, differentiation, and proliferation. Thus, the results demonstrate the significant advantage of the proposed model as well as the biophysical importance of exploring spatiotemporal dynamics of the coupling phenomena of patterning and deformation in 3D space..
3. Tetsuhisa Otani, Thanh Phuong Nguyen, Shinsaku Tokuda, Kei Sugihara, Taichi Sugawara, Kyoko Furuse, Takashi Miura, Klaus Ebnet, Mikio Furuse, Claudins and JAM-A coordinately regulate tight junction formation and epithelial polarity, The Journal of cell biology, 10.1083/jcb.201812157, 218, 10, 3372-3396, 2019.10, Tight junctions (TJs) establish the epithelial barrier and are thought to form a membrane fence to regulate epithelial polarity, although the roles of TJs in epithelial polarity remain controversial. Claudins constitute TJ strands in conjunction with the cytoplasmic scaffolds ZO-1 and ZO-2 and play pivotal roles in epithelial barrier formation. However, how claudins and other TJ membrane proteins cooperate to organize TJs remains unclear. Here, we systematically knocked out TJ components by genome editing and show that while ZO-1/ZO-2-deficient cells lacked TJ structures and epithelial barriers, claudin-deficient cells lacked TJ strands and an electrolyte permeability barrier but formed membrane appositions and a macromolecule permeability barrier. Moreover, epithelial polarity was disorganized in ZO-1/ZO-2-deficient cells, but not in claudin-deficient cells. Simultaneous deletion of claudins and a TJ membrane protein JAM-A resulted in a loss of membrane appositions and a macromolecule permeability barrier and in sporadic epithelial polarity defects. These results demonstrate that claudins and JAM-A coordinately regulate TJ formation and epithelial polarity..
4. Yuji Nashimoto, Ryu Okada, Sanshiro Hanada, Yuichiro Arima, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa, Vascularized cancer on a chip
The effect of perfusion on growth and drug delivery of tumor spheroid, Biomaterials, 10.1016/j.biomaterials.2019.119547, 229, 2020.01, Tumor vasculature creates a hostile tumor microenvironment (TME) in vivo and nourishes cancers, resulting in cancer progression and drug resistance. To mimic the biochemical and biomechanical environments of tumors in vitro, several models integrated with a vascular network have been reported. However, the tumor responses to biochemical and biomechanical stimuli were evaluated under static conditions and failed to incorporate the effects of blood flow to tumors. In this study, we present a tumor-on-a-chip platform that enables the evaluation of tumor activities with intraluminal flow in an engineered tumor vascular network. The fibroblasts in the tumor spheroid induced angiogenic sprouts, which constructed a perfusable vascular network in a tumor spheroid. The perfusability of the engineered vascular network was preserved during the culture. Moreover, perfusion for over 24 h significantly increased the proliferation activities of tumor cells and decreased cell death in the spheroid. Drug administration under perfusion condition did not show the dose-dependent effects of anticancer drugs on tumor activities in contrast to the results under static conditions. Our results demonstrate the importance of flow in a vascular network for the evaluation of tumor activities in a drug screening platform..
5. Nobuyuki Futai, Takanori Sano, Masahiro Sumita, Atsushi Takano, Ryuji Yokokawa, Takashi Miura, Vascular remodeling processes in an integrated microfluidic device, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, 1080-1081, 2020.01, We have succeeded in maintaining self-organized microvascular networks on-chip for over a month in a portable (71×26×28 mm) microfluidic co-culture system of endothelial cells and fibroblasts. We observed the long-term changes of microvascular networks in a microchannel. Perfusion through lumens may accelerate the remodeling processes. HUVECs formed stable lumens by around 100th day of cultivation, and the lumens could be maintained for at least 132 days..
6. Yuji Nashimoto, Yukako Teraoka, Akiko Nakamasu, Sanshiro Hanada, Yuichiro Arima, Yu Suke Torisawa, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa, Perfusion culture through a vasculature constructed in a tumor spheroid, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, 1029-1030, 2020.01, We developed a microfluidic system for a perfusion culture of tumor spheroids utilizing engineered vasculatures. By the definition of the cellular interaction between fibroblast cells in a tumor spheroid and endothelial cells in microchannels, we successfully induced angiogenic sprouts from the microchannels, which anastomosed with the spheroid and formed perfusable vasculatures. We continuously supplied medium to the tumor spheroid through the vasculature and evaluated the effect of perfusion on the growth of the tumor spheroid. Our perfusion system greatly improved the cellular proliferation activity, mimicking the nutrition supply and evacuation of waste product through vasculatures surrounding tumors in vivo..
7. Kei Sugihara, Saori Sasaki, Akiyoshi Uemura, Satoru Kidoaki, Takashi Miura, Mechanisms of endothelial cell coverage by pericytes
computational modelling of cell wrapping and in vitro experiments, Journal of the Royal Society, Interface, 10.1098/rsif.2019.0739, 17, 162, 2020.01, Pericytes (PCs) wrap around endothelial cells (ECs) and perform diverse functions in physiological and pathological processes. Although molecular interactions between ECs and PCs have been extensively studied, the morphological processes at the cellular level and their underlying mechanisms have remained elusive. In this study, using a simple cellular Potts model, we explored the mechanisms for EC wrapping by PCs. Based on the observed in vitro cell wrapping in three-dimensional PC-EC coculture, the model identified four putative contributing factors: preferential adhesion of PCs to the extracellular matrix (ECM), strong cell-cell adhesion, PC surface softness and larger PC size. While cell-cell adhesion can contribute to the prevention of cell segregation and the degree of cell wrapping, it cannot determine the orientation of cell wrapping alone. While atomic force microscopy revealed that PCs have a larger Young's modulus than ECs, the experimental analyses supported preferential ECM adhesion and size asymmetry. We also formulated the corresponding energy minimization problem and numerically solved this problem for specific cases. These results give biological insights into the role of PC-ECM adhesion in PC coverage. The modelling framework presented here should also be applicable to other cell wrapping phenomena observed in vivo..
8. Yukako Teraoka, Masamune Nakayama, Yuji Nashimoto, Akiko Nakamasu, Sanshiro Hanada, Yuichiro Arima, Yu Suke Torisawa, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa, Angiogenesis derived from shear stress optimised by a microfruidic device, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, 1072-1073, 2020.01, Previously, we created a 3D vascular network in a microfluidic device and demonstrated that the luminal flow significantly enhanced the formation of angiogenic sprouts as compared to static conditions [1]. These results could not be explained based on either biochemical factors (oxygen and nutrient transport) or mechanical factors (pressure and shear stress) derived angiogenesis. In this study, we investigated the effect of flow rate and shear stress on angiogenesis induced by luminal flow. We calculated the shear stress exposed on the endothelium where new sprouting initiated. Our results showed that angiogenesis was induced in the specific range of shear stress..
9. Scott Erickson, Hiroki Ihara, Sanshiro Hanada, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa, An On-Chip Vascular Network to Investigate Pericyte Migration and Intercellular Signaling, 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020, 10.1109/MEMS46641.2020.9056369, 444-447, 2020.01, Pericytes are critical support cells for vasculature. They aid in sprout formation, promote the expression of junction proteins between endothelial cells, and help regulate blood flow. But to perform these roles, they must first be recruited by endothelial cells. Here, we use a microchannel device to create self-assembling vasculature and study the effect of inhibiting pericyte-endothelial cell communication. We explore the effect of pericytes on vascular morphology and track pericyte coverage with and without the inhibitory drug, Imatinib. We find that pericyte migration and vessel morphology are changed with inhibition of platelet-derived growth factor (PDGF) receptor. In this way, we show the value of this system for both drug development and basic biological studies..
10. Han SK, Qi X, Sugihara K, Dang JH, Endo TA, Miller KL, Kim ED, Miura T, Torii KU., MUTE Directly Orchestrates Cell-State Switch and the Single Symmetric Division to Create Stomata., Dev Cell., doi: 10.1016/j.devcel.2018.04.010. , 45, 3, 303-315, 2018.05.
11. Shinoda T, Nagasaka A, Inoue Y, Higuchi R, Minami Y, Kato K, Suzuki M, Kondo T, Kawaue T, Saito K, Ueno N, Fukazawa Y, Nagayama M, Miura T, Adachi T, Miyata T. , Elasticity-based boosting of neuroepithelial nucleokinesis via indirect energy transfer from mother to daughter., PLoS Biol., 10.1371/journal.pbio.2004426, 16, 4, :e2004426, 2018.04.
12. Okuda S, Miura T, Inoue Y, Adachi T, Eiraku M., Combining Turing and 3D vertex models reproduces autonomous multicellular morphogenesis with undulation, tubulation, and branching., Sci Rep, 10.1038/s41598-018-20678-6., 8, 1, 2386, 2018.02.
13. Sasaki D, Nakajima H, Yamaguchi Y, Yokokawa R, Ei SI, Miura T., Mathematical modeling for meshwork formation of endothelial cells in fibrin gels. , J Theor Biol., 10.1016/j.jtbi.2017.06.012., 429, 95-104, 2017.10.
14. Nashimoto Y, Hayashi T, Kunita I, Nakamasu A, Torisawa YS, Nakayama M, Takigawa-Imamura H, Kotera H, Nishiyama K, Miura T, Yokokawa R., Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device., Integr Biol , doi: 10.1039/c7ib00024c., 9, 6, 506-518, 2017.06.
15. Yuki Matsunaga, Mariko Noda, Murakawa Hideki, Kanehiro Hayashi, Arata Nagasaka, Seika Inoue, Takaki Miyata, Takashi Miura, Ken-ichiro Kubo, Kazunori Nakajima, Reelin transiently promotes N-cadherin–dependent neuronal adhesion during mouse cortical development, Proceedings of the National Academy of Sciences , 10.1073/pnas.1615215114, 114, 8, 2048-2053, 2017.02.
16. Takumi Higaki, Hisako TAKIGAWA-IMAMURA, Kae Akita, Natsumaro Kutsuna, Ryo Kobayashi, Seiichiro Hasezawa, Takashi Miura, Exogenous Cellulase Switches Cell Interdigitation to Cell Elongation in an RIC1-dependent Manner in Arabidopsis thaliana Cotyledon Pavement Cells, Plant and Cell Physiology , https://doi.org/10.1093/pcp/pcw183, 58, 1, 106-119, 2016.12.
17. Kenji Yoshimura, Ryo Kobayash, Tomohisa Ohmura, Yoshinaga Kajimoto, Takashi Miura, A new mathematical model for pattern formation by cranial sutures, J theor biol, 408, 66-74, 2016.08.
18. Makoto Sato, Tetsuo Yasugi, Yoshiaki Minami, Takashi Miura, Masaharu Nagayama, Notch-mediated lateral inhibition regulates proneural wave propagation when combined with EGF-mediated reaction diffusion, PNAS, 113, 35, E5153-5162, 2016.06.
19. Takumi Higaki, Natsumaro Kutsuna, Kae Akita, Hisako TAKIGAWA-IMAMURA, Kenji Yoshimura, Takashi Miura, A Theoretical Model of Jigsaw-Puzzle Pattern Formation by Plant Leaf Epidermal Cells, PLoS Comp Biol, 12, 4, e1004833, 2016.04.
20. M Kobayashi‐Miura, Takashi Miura, H Osago, Y Yamaguchi, T Aoyama, T Tanabe, K-i Matsumoto, Y Fujita, Rat Articular Cartilages Change Their Tissue and Protein Compositions During Perinatal Period, Anatomia, histologia, embryologia, 45, 1, 9-18, 2014.10.
21. Takashi Miura, Turing and Wolpert Work Together During Limb Development, Science Signaling, scisignal., 2004038v1, 2013.04.
22. Ryuzo Arai, Masahiko Kobayashi, Yoshinobu Toda, Shinichiro Nakamura, Takashi Miura, Takashi Nakamura., "Fiber components of the shoulder superior labrum", , Surgical and Radiologic Anatomy:, Volume 34, , Issue 1 (2012),, Page 49-56., 2013.03.
23. Alvaro Kohn-Luque, Walter de Back, Yoshimi Yamaguchi, Kenji Yoshimura, Miguel A Herrero, Takashi Miura, Dynamics of VEGF matrix-retention in vascular network patterning, Physical biology, 10, 6, 066007, 2013.03.
24. Ryuzo Arai, Masahiko Kobayashi, Yoshinobu Toda, Shinichiro Nakamura, Takashi Miura, Takashi Nakamura, Fiber components of the shoulder superior labrum, Surgical and radiologic anatomy, 1-8, 2012.04.
25. Takashi Miura, How well does Turing's theory of morphogenesis work?, 数理研講究録, 1796, 31-38, 2012.03.
26. Takashi Miura, Modulation of activator diffusion by extracellular matrix in turing system, RIMS Kokyuroku Bessatsu, B 3,, 165-176, 2007.04.
27. Takashi Miura, Virtual embryology: a 3D library reconstructed from human embryo sections and animation of development process., Medinfo. MEDINFO, 8, 1229, 1995.06.