Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Shun-ichiro Takano Last modified date:2023.09.28

Assistant Professor / Department of Bioresource Sciences / Faculty of Agriculture

1. Shun‑ichiro Takano1 · Yoshinori Utsumi2 · Atsushi Nagano3 · Satoshi Takahashi2 · Akihiro Ezoe2 · Motoaki Seki2,4,5 · Thi Xuyen Le6 · Keiji Takasu1, Induction of leaf curling in cassava plants by the cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae), Applied Entomology and Zoology , 10.1007/s13355-023-00832-x, 2023.06.
2. Shun-ichiro Takano, Yasuhiro Gotoh, Tetsuya Hayashi, “Candidatus Mesenet longicola”: Novel Endosymbionts of Brontispa longissima that Induce Cytoplasmic Incompatibility, Microbial Ecology, 10.1007/s00248-021-01686-y, 2021.01, Intracellular bacteria that are mainly transmitted maternally affect their arthropod hosts’ biology in various ways. One such effect
is known as cytoplasmic incompatibility (CI), and three bacterial species are known to induce CI: Wolbachia, Cardinium hertigii,
and a recently found alphaproteobacterial symbiont. To clarify the taxonomic status and provide the foundation for future studies
to reveal CI mechanisms and other phenotypes, we investigated genetic and morphological properties of the third CI inducer that
we have previously reported inducing CI in the coconut beetle Brontispa longissima. The draft genome of the bacteria was
obtained from the oocytes of two isofemale lines of B. longissima infected with the bacteria: one from Japan (GL2) and the other
from Vietnam (L5). Genome features of the symbionts (sGL2 and sL5) were highly similar, showing 1.3 Mb in size, 32.1% GC
content, and 99.83% average nucleotide sequence. A phylogenetic study based on 43 universal and single-copy phylogenetic
marker genes indicates that they formed a distinct clade in the family Anaplasmataceae. 16S rRNA gene sequences indicate that
they are different from the closest known relatives, at least at the genus level. Therefore, we propose a new genus and species,
“Candidatus Mesenet longicola”, for the symbionts of B. longissima. Morphological analyses showed that Ca. M. longicola is an
intracellular bacterium that is ellipsoidal to rod-shaped and 0.94 ± 0.26 μm (mean ± SD) in length, and accumulated in the
anterior part of the oocyte. Candidates for the Ca. M. longicola genes responsible for CI induction are also described..
3. Shun-ichiro Takano, Nancy A. Schellhorn, Host discrimination of a larval parasitoid: the quick movement of Microplitis demolitor, BioControl,, 2021.01, [URL].
4. Dat Nguyen Tuan, Layheng Sam, Chi Zhang, Chau Nguyen Ngoc Bao, Shun-ichiro Takano, Keiji Takasu, Taro Colocasia esculenta as an alternative host plant for rearing cassava mealybug (Hemiptera: Pseudococcidae) and its parasitoid Anagyrus lopezi (Hymenoptera: Encyrtidae), Applied Entomology and Zoology, 10.1007/s13355-020-00690-x, 2020.06.
5. Shun-ichiro Takano, Keiji Takasu, Food deprivation increases reproductive effort in a parasitoid wasp, Biological Control, 133, 75-80, 2019.03.
6. Takasu K, Ndabarua JN, Pham HT, Takano S, Pupal-adult parasitism of the coconut hispine beetle by the koinobiont pupal parasitoid Tetrastichus brontispae, Biological Control, 10.1016/j.biocontrol.2018.07.013, 126, 90-96, 2018.11.
7. Chomphukhiao N, Takano S, Takasu K, Uraichuen S, Existence of two strains of Habrobracon hebetor (Hymenoptera: Braconidae): a complex in Thailand and Japan, Applied Entomology and Zoology, 10.1007/s13355-018-0568-6, 53, 3, 373-380, 2018.07.
8. Takano S, Tuda M, Takasu K, Furuya N, Imamura Y, Kim S, Tashiro K, Iiyama K, Tavares M, Amaral AC, Unique clade of alphaproteobacterial endosymbionts induces complete cytoplasmic incompatibility in the coconut beetle, PNAS, 10.1073/pnas.1618094114, 114, 23, 6110-6115, 2017.06, 節足動物に感染して増殖する細菌は、自身の繁殖のため様々な方法で宿主の生殖を操作することが知られている。細菌に感染した雌が雄と交尾した時、雄が感染しているかどうかにかかわらず、雌の卵は発育する。しかし、非感染雌が感染雄と交尾した時、その雌の卵は孵化しない。この現象は細胞質不和合性(CI)と呼ばれている。本研究ではココヤシ新葉を加害する侵入害虫として知られる、節足動物キムネクロナガハムシから、完全なCIを引き起こす新たな細菌(以下、細菌L)を発見した。キムネクロナガハムシには遺伝子の異なる2つの系統(Di系統とLp系統)が存在する。Di系統雌とLp系統雄は交尾するが、産まれた卵は全くふ化しない。しかし、今回の研究で、抗生物質処理によりLp雄体内に共生する細菌Lを除去すると、卵は高い率でふ化するようになることが分かった。今回発見された細菌Lは、完全なCIを引き起こす細菌としては1970年代初頭に発見されたボルバキアに次いで世界で2例目となる(不完全なCIを引き起こす細菌としてはカルディニウムが知られているため、CIを引き起こす細菌としては世界で3例目となる)。今後、細菌Lを他の害虫に感染させることができれば、将来的には害虫防除に利用できる可能性がある。例えば、害虫が発生している地域に室内で増殖した大量の感染雄を放すと、野生の非感染雄が野生の雌と交尾する機会が減り、害虫個体数が激減することが期待される。.
9. Takano S, Takasu K, Tavares M, Gusmao M, Amaral AC, Differences in invasiveness between two cryptic species of the coconut beetle Brontispa longissima in Timor-Leste, Biological Invasions, 10.1007/s10530-017-1394-4, 19, 6, 1839-1851, 2017.06.
10. Takano S, Takasu K, Ability of Megacopta cribraria (Hemiptera: Plataspidae) to cling to different surfaces against extreme wind, Journal of Insect Behavior, 10.1007/s10905-016-9557-1, 29, 256-261, 2016.06.
11. Takano S, Survival of Bactrocera latifrons (Diptera: Tephritidae) adults under constant and fluctuating low temperatures, Applied Entomology and Zoology, 10.1007/s13355-014-0263-1, 49, 3, 411-419, 2014.08.
12. Takano S, Takasu K, Fushimi T, Ichiki RT, Nakamura S, Suitability of four palm species for the development of the invasive pest Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae), Entomological Science, 17, 265-268, 2014.04.
13. Takano S, Takasu K, Murata M, Huong NT, Nakamura S, Comparative developmental and reproductive biology of geographical populations from two cryptic species in Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae), Entomological Science, 16, 3, 335-340, 2013.07.
14. Takano S, Mochizuki A, Konishi K, Takasu K, Alouw JC, Pandin DS, Nakamura S, Rapid discrimination of two cryptic species within Brontispa longissima (Coleoptera: Chrysomelidae) by PCR-RFLP, Journal of Pest Science, 86, 2, 151-155, 2013.06.
15. Htwe AN, Murata M, Takano S, Nakamura S, Effects of constant and fluctuating temperatures on development of the coconut hispine beetle, Brontispa longissima (Coleoptera: Chrysomelidae) and two species of parasitoid, Biocontrol Science & Technology, 23, 5, 574-583, 2013.05.
16. Kawazu K, Sugeno W, Mochizuki A, Takano S, Murata M, Ichiki RT, Nakamura S, Age in relation to copulation, egg development, and multiple mating behavior in the coconut palm pest Brontispa longissima (Coleoptera: Chrysomelidae), Annals of the Entomological Society of America, 105, 5, 746-750, 2012.09.
17. Huong N. T., Oo TT, Ichiki RT, Takano S, Murata M, Takasu K, Konishi K, Tunkumthong S, Chomphookhiaw N, Nakamura S, Parasitization of Tetrastichus brontispae (Hymenoptera: Eulophidae), a biological control agent of the coconut hispine beetle Brontispa longissima (Coleoptera: Chrysomelidae), Biocontrol Science & Technology, 22, 8, 955-968, 2012.08.
18. Takano S, Takasu K, Ichiki RT, Nakamura S, Cold tolerance of the coconut hispine beetle, Brontispa longissima (Coleoptera: Chrysomelidae) in Japan, Applied Entomology and Zoology, 47, 3, 173-180, 2012.08.
19. Murata M, Htwe AN, Takano S, Huong NT, Ichiki RT, Sugeno W, Mochizuki A, Nakamura S, A comparison of artificial diets for mass rearing Brontispa longissima (Coleoptera: Chrysomelidae) as hosts for the larval and the pupal parasitoids, Journal of Economic Entomology, 105, 3, 802-809, 2012.05.
20. Murata M, Dung DT, Takano S, Ichiki RT, Nakamura S, Effect of larval density on development of the coconut hispine beetle, Brontispa longissima (Getro) (Coleoptera: Chrysomelidae), Psyche, Article ID 981475, 2012.02.
21. Takano S, Takasu K, Fushimi T, Ichiki RT, Nakamura S, Life history traits and damage potential of an invasive pest Brontispa longissima (Coleoptera: Chrysomelidae) on Satakentia liukiuensis, Entomological Science, 15, 2, 238-245, 2012.04.
22. Ichiki RT, Murata M, Takano S, Oo T. T, Nguyen H. T, Suasa-ard W, Uraichuen S, Nakamura S, Artificial diets for rearing the coconut hispine beetle, Brontispa longissima (Coleoptera: Chrysomelidae), and their suitability to two specialist parasitoids, Biocontrol Science & Technology, 21, 12, 1423-1435, 2011.12.
23. Takano S, Takasu K, Ichiki RT, Fushimi T, Nakamura S, Induction of host-plant preference in Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae), Journal of Applied Entomology, 135, 8, 634-640, 2011.09.
24. Sugeno W, Kawazu K, Takano S, Nakamura S, Mochizuki A, Suitability of monocots for rearing alien coconut pest Brontispa longissima (Coleoptera: Chrysomelidae), Annals of the Entomological Society of America, 104, 4, 682-687, 2011.07.
25. Takano S, Mochizuki A, Konishi K, Takasu K, Alouw JC, Pandin DS, Nakamura S, Two cryptic species in Brontispa longissima (Coleoptera: Chrysomelidae): evidence from mitochondrial DNA analysis and crosses between the two nominal species, Annals of the Entomological Society of America, 104, 2, 121-131, 2011.03.
26. Takasu K, Takano S, Konishi K, Nakamura S, An invasive pest Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae) attacks an endemic palm in the Yaeyama Islands, Japan, Applied Entomology and Zoology, 45, 1, 137-144, 2010.03.
27. Ichiki RT, Dung DT, Takano S, Nakamura S, Artificial diets for rearing the coconut hispine beetle Brontispa longissima and its parasitoid Asecodes hispinarum, Journal of Applied Entomology, 133, 7, 539-545, 2009.08.
28. Takano S, Iwaizumi R, Nakanishi Y, Someya H, Laboratory hybridization between the two clades of Liriomyza huidobrensis (Diptera: Agromyzidae), Applied Entomology and Zoology, 43, 3, 397-402, 2008.08.
29. Takasu K, Takano S, Mizutani N, Wada T, Flight orientation behavior of Ooencyrtus nezarae (Hymenoptera: Encyrtidae), an egg parasitoid of phytophagous bugs in soybean, Entomolgical Science, 7, 3, 201-206, 2004.09.
30. Takasu K, Takano S, Sasaki M, Yagi S, Nakamura S, Host Recognition by the Tick Parasitoid Ixodiphagus hookeri (Hymenoptera: Encyrtidae), Environmental Entomology, 32, 3, 614-617, 2003.06.
31. Shun-ichiro TAKANO, Biological control of the coconut beetle: classical biological control in southeast Asia and finding a new biocontrol agent that belongs to a novel clade of Alphaproteobacteria, Entomological Society of Queensland News Bulletin, 45, 6, 112-114, 2017.09.
32. Iwaizumi R, Arakawa K, Takano S, Someya H, Report on the Occurrence and control of the Asian Gypsy moth (AGM), Lymantria dispar (Linnaeus) (Lepidoptera: Lymantriidae) in port Areas of Japan, Research Bulletin of the Plant Protection Service Japan, 45, 29-36, 2009.03.