Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Yuki Sato Last modified date:2021.05.29

Associate Professor / Department of Basic Medicine / Faculty of Medical Sciences


Papers
1. Alvin R. Acebedo, Kentaro Suzuki, Shinjiro Hino , Mellissa C. Alcantara, Yuki Sato, Hisashi Haga, Ken-ichi Matsumoto, Mitsuyoshi Nakao, Kenji Shimamura, Toru Takeo, Naomi Nakagata, Sinichi Miyagawa, Ryuichi Nishinakamura, Robert S. Adelstein, Gen Yamada, Mesenchymal actomyosin contractility is required for androgen-driven urethral masculinization in mice, Communications Biology, 10.1038/s42003-019-0336-3, 2, 95, s42003, 2019.03.
2. Liqing Liu L, Kentaro Suzuki,Einice Chun, Aki Murashima, Yuki Sato, Naomi Nakagata, Toshihiko Fujimori, Sigenobu Yonemura, Wanzhong He, Gen Yamada, Androgen regulates dimorphic F-actin assemblies in the genital organogenesis, Sexual Development, 10.1159/000477452, 11, 190-202, 2017.07.
3. Yuki Sato, Kei Nagatoshi, Ayumi Hamano, Yuko Imamura, David Huss, Seiichi Uchida, Rusty Lansford, Basal filopodia and vascular mechanical stress organize fibronectin into pillars bridging the mesoderm-endoderm gap, Development (Cambridge), 10.1242/dev.141259, 144, 2, 281-291, 2017.01, Cells may exchange information with other cells and tissues by exerting forces on the extracellular matrix (ECM). Fibronectin (FN) is an important ECM component that forms fibrils through cell contacts and creates directionally biased geometry. Here, we demonstrate that FN is deposited as pillars between widely separated germ layers, namely the somitic mesoderm and the endoderm, in quail embryos. Alongside the FN pillars, long filopodia protrude from the basal surfaces of somite epithelial cells. Loss-of-function of Ena/VASP, α5β1-integrins or talin in the somitic cells abolished the FN pillars, indicating that FN pillar formation is dependent on the basal filopodia through these molecules. The basal filopodia and FN pillars are also necessary for proper somite morphogenesis. We identified a new mechanism contributing to FN pillar formation by focusing on cyclic expansion of adjacent dorsal aorta. Maintenance of the directional alignment of the FN pillars depends on pulsatile blood flow through the dorsal aortae. These results suggest that the FN pillars are specifically established through filopodia-mediated and pulsating force-related mechanisms..
4. Shoichiro Kanda, Shunsuke Tanigawa, Tomoko Ohmori, Atsuhiro Taguchi, Kuniko Kudo, Yutaka Suzuki, Yuki Sato, Shinjiro Hino, Maike Sander, Alan O. Perantoni, Sumio Sugano, Mitsuyoshi Nakao, Ryuichi Nishinakamura, Sall1 maintains nephron progenitors and nascent nephrons by acting as both an activator and a repressor, Journal of the American Society of Nephrology : JASN, 10.1681/ASN.2013080896, 25, 11, 2584-2595, 2014.11, The balanced self-renewal and differentiation of nephron progenitors are critical for kidney development and controlled, in part, by the transcription factor Six2, which antagonizes canonical Wnt signaling-mediated differentiation. A nuclear factor, Sall1, is expressed in Six2-positive progenitors as well as differentiating nascent nephrons, and it is essential for kidney formation. However, the molecular functions and targets of Sall1, especially the functions and targets in the nephron progenitors, remain unknown. Here, we report that Sall1 deletion in Six2-positive nephron progenitors results in severe progenitor depletion and apoptosis of the differentiating nephrons inmice. Analysis ofmice with an inducible Sall1 deletion revealed that Sall1 activates genes expressed in progenitors while repressing genes expressed in differentiating nephrons. Sall1 and Six2 co-occupied many progenitor-related gene loci, and Sall1 bound to Six2 biochemically. In contrast, Sall1 did not bind to the Wnt4 locus suppressed by Six2. Sall1-mediated repressionwas also independent of its binding to DNA. Thus, Sall1maintains nephron progenitors and their derivatives by a unique mechanism, which partly overlaps but is distinct fromthat of Six2: Sall1 activates progenitor-related genes in Six2-positive nephron progenitors and represses gene expression in Six2-negative differentiating nascent nephrons..
5. Yuki Sato, Greg Poynter, David Huss, Michael B. Filla, Andras Czirok, Brenda J. Rongish, Charles D. Little, Scott E. Fraser, Rusty Lansford, Dynamic analysis of vascular morphogenesis using transgenic quail embryos., PLoS One, 5, 9, 2010.09, One of the least understood and most central questions confronting biologists is how initially simple clusters or sheet-like cell collectives can assemble into highly complex three-dimensional functional tissues and organs. Due to the limits of oxygen diffusion, blood vessels are an essential and ubiquitous presence in all amniote tissues and organs. Vasculogenesis, the de novo self-assembly of endothelial cell (EC) precursors into endothelial tubes, is the first step in blood vessel formation. Static imaging and in vitro models are wholly inadequate to capture many aspects of vascular pattern formation in vivo, because vasculogenesis involves dynamic changes of the endothelial cells and of the forming blood vessels, in an embryo that is changing size and shape. We have generated Tie1 transgenic quail lines Tg(tie1:H2B-eYFP) that express H2B-eYFP in all of their endothelial cells which permit investigations into early embryonic vascular morphogenesis with unprecedented clarity and insight. By combining the power of molecular genetics with the elegance of dynamic imaging, we follow the precise patterning of endothelial cells in space and time. We show that during vasculogenesis within the vascular plexus, ECs move independently to form the rudiments of blood vessels, all while collectively moving with gastrulating tissues that flow toward the embryo midline. The aortae are a composite of somatic derived ECs forming its dorsal regions and the splanchnic derived ECs forming its ventral region. The ECs in the dorsal regions of the forming aortae exhibit variable mediolateral motions as they move rostrally; those in more ventral regions show significant lateral-to-medial movement as they course rostrally. The present results offer a powerful approach to the major challenge of studying the relative role(s) of the mechanical, molecular, and cellular mechanisms of vascular development. In past studies, the advantages of the molecular genetic tools available in mouse were counterbalanced by the limited experimental accessibility needed for imaging and perturbation studies. Avian embryos provide the needed accessibility, but few genetic resources. The creation of transgenic quail with labeled endothelia builds upon the important roles that avian embryos have played in previous studies of vascular development..
6. Emi Ohata, Ryosuke Tadokoro, Yuki Sato, Daisuke Saito, Yoshiko Takahashi, Notch signal is sufficient to direct an endothelial conversion from non-endothelial somitic cells conveyed to the aortic region by CXCR4, Developmental Biology, 10.1016/j.ydbio.2009.08.010, 335, 1, 33-42, 2009.11, During the early formation of the dorsal aorta, the first-forming embryonic vessel in amniotes, a subset of somitic cells selected as presumptive angioblasts, migrates toward the dorsal aorta, where they eventually differentiate into endothelial cells. We have recently shown that these processes are controlled by Notch signals (Sato, Y., Watanabe, T., Saito, D., Takahashi, T., Yoshida, S., Kohyama, J., Ohata, E., Okano, H., and Takahashi, Y., 2008. Notch mediates the segmental specification of angioblasts in somites and their directed migration toward the dorsal aorta in avian embryos. Dev. Cell 14, 890-901.). Here, we studied a possible link between Notch and chemokine signals, SDF1/CXCR4, the latter found to be dominantly expressed in developing aorta/somites. Although CXCR4 overexpression caused a directed migration of somitic cells to the aortic region in a manner similar to Notch, no positive epistatic relationships between Notch and SDF1/CXCR4 were detected. After reaching the aortic region, the CXCR4-electroporated cells exhibited no endothelial character. Importantly, however, once provided with Notch activity, they could successfully be incorporated into developing vessels as endothelial cells. These findings were obtained combining the tetracycline-inducible gene expression method with the transposon-mediated stable gene transfer technique. We conclude that Notch activation is sufficient to direct naïve mesenchymal cells to differentiate into endothelial cells once the cells are conveyed to the aortic region..
7. Tadayoshi Watanabe, Yuki Sato, Daisuke Saito, Ryosuke Tadokoro, Yoshiko Takahashi, EphrinB2 coordinates the formation of a morphological boundary and cell epithelialization during somite segmentation, Proceedings of the National Academy of Sciences of the United States of America, 10.1073/pnas.0902859106, 106, 18, 7467-7472, 2009.05, During early morphogenesis, tissue segregation is often accompanied by changes in cell shape. To understand how such coordination is regulated, somitogenesis was used as a model. When a somite forms in the anterior end of the presomitic mesoderm, an intersomitic boundary (gap) emerges, and it is rapidly followed by cell epithelialization at this border. It has been known that the gap formation is regulated by intercellular signals. We here demonstrate that cMeso-1, the chicken homolog of mouse Mesp2, upregulates EphA4 in the cells located posteriorly to a forming boundary. This in turn activates EphrinB2-reverse signals in the anteriorly juxtaposed cells, where the EphrinB2 signal is sufficient to cause a gap formation and cell epithelialization cell-autonomously. During these processes, Cdc42 needs to be repressed via tyrosine phosphorylation of EphrinB2. This is the first demonstration that Ephrin-reverse signal acts as a platform that couples distinct morphogenetic changes in cell polarity and tissue shape..
8. Yuki Sato, Tadayoshi Watanabe, Daisuke Saito, Teruaki Takahashi, Shosei Yoshida, Jun Kohyama, Emi Ohata, Hideyuki Okano, Yoshiko Takahashi, Notch signaling mediates the segmental specification of angioblasts in somites and their directed migration toward the dorsal aorta in avian embryos, Developmental Cell, 10.1016/j.devcel.2008.03.024, 14, 890-901, 2008.06.
9. Yuki Sato, Toshiharu Kasai, Shinichi Nakagawa, Koji Tanabe, Koichi Kawakami, Yoshiko Takahashi , Stable integration and conditional expression of electroporated transgenes in chicken embryos, Developmental Biology, 10.1016/j.ydbio.2007.01.043, 305, 616-624, 2007.02.
10. Yumiko Oka, Yuki Sato, Hokari Tsuda, Kazunori Hanaoka, Yohei Hirai, Yoshiko Takahashi, Epimorphin acts extracellularly to promote cell sorting and aggregation during the condensation of vertebral cartilage, Developmental Biology, 10.1016/j.ydbio.2005.12.001, 291, 1, 25-37, 2006.03, Formation of vertebrae occurs via endochondral ossification, a process involving condensation of precartilaginous cells. Here, we provide the first molecular evidence of mechanism that underlies initiation of this process by showing that the extracellular factor, Epimorphin, plays a role during early steps in vertebral cartilage condensation. Epimorphin mRNA is predominantly localized in the vertebral primordium. When provided exogenously in ovo, it causes precocious differentiation of chondrocytes, resulting in the formation of supernumerary vertebral cartilage in chicken embryos. To further analyze its mode of action, we used an in vitro co-culture system in which labeled 10T1/2 or sclerotomal prechondrogenic cells were co-cultured with unlabeled Epimorphin-producing cells. In the presence of Epimorphin, the labeled cells formed tightly packed aggregates, and sclerotomal cells displayed augmented accumulation of NCAM and other early markers of chondrocyte differentiation. Finally, we found that the Epimorphin expression is initiated during vertebrogenesis by Sonic hedgehog from the notochord mediated by Sox 9. We present a model in which successive action of Epimorphin in recruiting and stacking sclerotomal cells leads to a sequential elongation of a vertebral primordium..
11. Yuki Sato, Yoshiko Takahashi, A novel signal induces a segmentation fissure by acting in a ventral-to-dorsal direction in the presomitic mesoderm, Developmental Biology, 10.1016/j.ydbio.2005.03.007, 282, 1, 183-191, 2005.06, We describe here a novel inductive action that operates during somitic segmentation in chicken embryos. We previously reported that the posterior border cells located at a next-forming boundary in the anterior end of the presomitic mesoderm (PSM) exhibit an inductive activity that acts on the anterior cells to cause the formation of a somitic fissure (Sato, Y., Yasuda, K., Takahashi, Y., 2002. Morphological boundary forms by a novel inductive event mediated by Lunatic fringe and Notch during somitic segmentation. Development 129, 3633-3644). In this study, we have found a second inductive action along the dorso-ventral (D-V) axis during fissure formation. When relocated into a non-segmenting region of PSM, the ventral-most cells taken from the presumptive boundary are sufficient to induce an ectopic fissure in host cells. The ventrally derived signal acts in a ventral-to-dorsal direction but not ventrally, regardless of where the ventral cells are placed. This directional signaling is governed, at least in part, by the signal-receiving cells of the PSM, which we found to be polarized along the D-V axis, and also by intimate cell-cell interactions. Finally, we have observed that morphological segmentation is able to rearrange the anterior and posterior regionalization of individual somites. These findings suggest that discrete unidirectional signals along both the antero-posterior and the D-V axes act coordinately to achieve the formation of the intersomitic fissure, and also that fissure formation is important for the fine-tuning of A-P regionalization in individual somites..
12. Rinako Suetsugu, Yuki Sato, Yoshiko Takahashi, Pax 2 expression in mesodermal segmentation and its relationship with EphA4 and Lunatic-fringe during chicken somitogenesis, Mechanisms of Development, 10.1016/S0925-4773(03)00109-6, 119, SUPPL. 1, 2002.12, In the Pax gene family, which encodes DNA-binding proteins, Pax 2 has been known to play important roles in the formation of the midbrain/hindbrain boundary, eye, inner ear and kidney in vertebrates (Bioessays 19 (1997) 755). In this article, we report a segmentally regulated pattern of Pax 2 expression during chicken somitogenesis. Pax 2 mRNA is localized in the rostral end of the unsegmented presomitic mesoderm (PSM), abutting anteriorly on a prospective segmentation border. This pattern repeats every segmentation cycle (90 min) observed in ovo and also in the hall embryo culture assay in which one hall of PSM along the midline is fixed immediately while the other half is cultured for a given period. We also determined the sequence of changes in Pax 2 expression during a segmentation cycle by comparing the pattern of Pax 2 with that of Lunatic-fringe (L-fringe), known to cycle periodically in posterior PSM. A systematic comparison of the expression patterns between Pax 2, L-fringe and EphA4 further highlighted a close relationship between EphA4 and Pax 2 during a segmentation cycle. Lastly, Pax 2 is not segmentally expressed in mouse PSM, suggestive of species (avian)-specific mechanisms underlying somitic segmentation..
13. Rinako Suetsugu, Yuki Sato, Yoshiko Takahashi, Pax 2 expression in mesodermal segmentation and its relationship with EphA4 and Lunatic-fringe during chicken somitogenesis, Gene Expression Patterns, 10.1016/S0925-4773(02)00344-1, 2, 1-2, 157-161, 2002.11, In the Pax gene family, which encodes DNA-binding proteins, Pax 2 has been known to play important roles in the formation of the midbrain/hindbrain boundary, eye, inner ear and kidney in vertebrates (Bioessays 19 (1997) 755). In this article, we report a segmentally regulated pattern of Pax 2 expression during chicken somitogenesis. Pax 2 mRNA is localized in the rostral end of the unsegmented presomitic mesoderm (PSM), abutting anteriorly on a prospective segmentation border. This pattern repeats every segmentation cycle (90 min) observed in ovo and also in the half embryo culture assay in which one half of PSM along the midline is fixed immediately while the other half is cultured for a given period. We also determined the sequence of changes in Pax 2 expression during a segmentation cycle by comparing the pattern of Pax 2 with that of Lunatic-fringe (L-fringe), known to cycle periodically in posterior PSM. A systematic comparison of the expression patterns between Pax 2, L-fringe and EphA4 further highlighted a close relationship between EphA4 and Pax 2 during a segmentation cycle. Lastly, Pax 2 is not segmentally expressed in mouse PSM, suggestive of species (avian)-specific mechanisms underlying somitic segmentation..
14. Yuki Sato, Kunio Yasuda, Yoshiko Takahashi, Morphological boundary forms by a novel inductive event mediated by Lunatic fringe and Notch during somitic segmentation, Development (Cambridge), 129, 15, 3633-3644, 2002.08, Boundary formation plays a central role in differentiating the flanking regions that give rise to discrete tissues and organs during early development. We have studied mechanisms by which a morphological boundary and tissue separation are regulated by examining chicken somite segmentation as a model system. By transplanting a small group of cells taken from a presumptive border into a non-segmentation site, we have found a novel inductive event where posteriorly juxtaposed cells to the next-forming border instruct the anterior cells to become separated and epithelialized. We have further studied the molecular mechanisms underlying these interactions by focusing on Lunatic fringe, a modulator of Notch signaling, which is expressed in the region of the presumptive boundary. By combining DNA in ovo electroporation and embryonic transplantation techniques we have ectopically made a sharp boundary of Lunatic fringe activity in the unsegmented paraxial mesoderm and observed a fissure formed at the interface. In addition, a constitutive active form of Notch mimics this instructive phenomenon. These suggest that the boundary-forming signals emanating from the posterior border cells are mediated by Notch, the action of which is confined to the border region by Lunatic fringe within the area where mRNAs of Notch and its ligand are broadly expressed in the presomitic mesoderm..