| 1. |
Takahashi A, Nagata M, Gupta A, Matsushita Y, Yamaguchi T, Mizuhashi K, Maki K, Ruellas AC, Cevidanes LS, Kronenberg HM, Ono N, Ono W., Autocrine regulation of mesenchymal progenitor cell fates orchestrates tooth eruption., Proc Natl Acad Sci U S A. , 2019.06. |
| 2. |
Jing-Qi Zhang, Akira Takahashi, Jiong-Yan Gu, Xiaoxu Zhang, Yukari Kyumoto-Nakamura, Akiko Kukita, Norihisa Uehara, Hidenobu Hiura, Takayoshi Yamaza, and Toshio Kukita, In vitro and in vivo detection of tunneling nanotubes in normal and pathological osteoclastogenesis involving osteoclast fusion, Laboratory Investigation, 2021.04. |
| 3. |
Yuri Matsuura, Atsuta Ikiru, Yasunori Ayukawa, Takayoshi Yamaza, Ryosuke Kondo, akira takahashi, Nobuyuki Ueda, Wakana Oshiro, Yoshihiro Tsukiyama, Kiyoshi Koyano, Therapeutic interactions between mesenchymal stem cells for healing medication-related osteonecrosis of the jaw, Stem Cell Research and Therapy, 10.1186/s13287-016-0367-3, 7, 1, 2016.08, Background: Mesenchymal stem cells (MSCs) have been isolated from a variety of tissues, including bone marrow, adipose, and mucosa. MSCs have the capacity for self-renewal and differentiation. Reports have been published on the systemic administration of MSCs leading to functional improvements by engraftment and differentiation, thus providing a new strategy to regenerate damaged tissues. Recently, it has become clear that MSCs possess immunomodulatory properties and can therefore be used to treat diseases. However, the therapeutic effect mechanisms of MSCs are yet to be determined. Here, we investigated these mechanisms using a medication-related osteonecrosis of the jaw (MRONJ)-like mouse model. Methods: To generate MRONJ-like characteristics, mice received intravenous zoledronate and dexamethasone two times a week. At 1 week after intravenous injection, maxillary first molars were extracted, and at 1 week after tooth extraction, MSCs were isolated from the bone marrow of the mice femurs and tibias. To compare "diseased MSCs" from MRONJ-like mice (d-MSCs) with "control MSCs" from untreated mice (c-MSCs), the isolated MSCs were analyzed by differentiation and colony-forming unit-fibroblast (CFU-F) assays and systemic transplantation of either d-MSCs or c-MSCs into MRONJ-like mice. Furthermore, we observed the exchange of cell contents among d-MSCs and c-MSCs during coculture with all combinations of each MSC type. Results: d-MSCs were inferior to c-MSCs in differentiation and CFU-F assays. Moreover, the d-MSC-treated group did not show earlier healing in MRONJ-like mice. In cocultures with any combination, MSC pairs formed cell-cell contacts and exchanged cell contents. Interestingly, the exchange among c-MSCs and d-MSCs was more frequently observed than other pairs, and d-MSCs were distinguishable from c-MSCs. Conclusions: The interaction of c-MSCs and d-MSCs, including exchange of cell contents, contributes to the treatment potential of d-MSCs. This cellular behavior might be one therapeutic mechanism used by MSCs for MRONJ.. |
| 4. |
Toshio Kukita, akira takahashi, Jing Qi Zhang, Akiko Kukita, Membrane nanotube formation in osteoclastogenesis, Cell Fusion
Overviews and Methods: Second Edition, 10.1007/978-1-4939-2703-6_14, 193-202, 2015.05, Membrane tunneling nanotubes (TNTs) are unique intercellular structures, which enable rapid transport of various materials and rapid communication between cells present in a long distance. During osteoclastogenesis, mononuclear osteoclast precursors form abundant TNTs in prior to cell-cell fusion. Here we introduce a protocol for detecting TNTs during osteoclastogenesis by use of live cell imaging utilizing a confocal laser microscopy. We also demonstrate a standard protocol for observation of TNTs by scanning electron microscope.. |
| 5. |
Toshio Kukita, akira takahashi, Jing Qi Zhang, Akiko Kukita, Membrane nanotube formation in osteoclastogenesis, Methods in Molecular Biology, 10.1007/978-1-4939-2703-6_14, 1313, 193-202, 2015.01, Membrane tunneling nanotubes (TNTs) are unique intercellular structures, which enable rapid transport of various materials and rapid communication between cells present in a long distance. During osteoclas-togenesis, mononuclear osteoclast precursors form abundant TNTs in prior to cell–cell fusion. Here we introduce a protocol for detecting TNTs during osteoclastogenesis by use of live cell imaging utilizing a confocal laser microscopy. We also demonstrate a standard protocol for observation of TNTs by scanning electron microscope.. |
