飼育下での板鰓類繁殖に向けた性周期の解明
キーワード：板鰓類、性ホルモン、サメ
2017.02.
魚類B細胞による自然免疫活性化機構
キーワード：魚類免疫、B細胞、自然免疫、自然抗体
2015.12.
魚類栓球の貪食能と炎症・抗炎症の調節機構
キーワード：魚類免疫、自然免疫、貪食細胞、栓球
2015.12.
主要学会発表等
| 1. |
Takahiro Nagasawa, Tomonori Somamoto, and Miki Nakao, TLR-MEDIATED TYPE-I INTERFERON PRODUCTION AND THE REGULATORY MECHANISMS IN CARP THROMBOCYTES, International conference on Fish & Shellfish Immunology 2019, 2019.06. |
| 2. |
Takahiro Nagasawa, Tomonori Somamoto, Miki Nakao, VIRAL LIGANDS RECOGNITION OF CARP THROMBOCYTES AS NATURAL TYPE I INTERFERON PRODUCING CELLS., 14th congress of International Society of Developmental and Comparative Immunology, 2018.06, In early stage of viral infection, viral-specific nucleic acids including single/double-stranded RNA (ss/dsRNA) are recognized by innate pattern recognition receptors, such as Toll-like receptors (TLRs), thereby type I interferon (IFN) is produced. In the present study, we report that thrombocytes in common carp (Cyprinus carpio) have potent ability to produce type I IFN in response to synthetic viral molecule mimicries. Magnetic-sorted mAb+ thrombocytes and other peripheral blood leukocytes (PBLs) were incubated with polyinosinic:polycytidylic acid (poly(I:C), an analog of dsRNA), resiquimod (R-848, an agonist of TLR7/8 which detect ssRNA), or CpG motif oligodeoxynucleotides. qPCR analysis showed that thrombocytes had produced large amount of type-I IFN compared with the PBL fraction. R-848 acutely activated the IFN production in both fractions, whereas increasing of the IFN expression by poly(I:C) were detected only in the PBL fractions. CpG DNA never induced type-I IFN expression but upregulated several inflammatory cytokines such as IL-6, suggesting that different signal pathways were activated by those ligand recognitions. After the R-848 stimulation, expression level of interferon-regulatory factor 7 (IRF7) were drastically increased in thrombocytes, suggesting that thrombocytes activated the IRF7-signal pathway to produce the IFN. Those pattern-recognition characteristics, type-I IFN producing capacity, and existence of the specialized IFN-producing pathway of carp thrombocytes are analogous to those of mammalian plasmacytoid dendritic cells, which are specialized leukocytes against viral infection, indicating that thrombocytes are important components for antiviral immunity in bony fish and can be a new target for the strategy of vaccine development.. |
| 3. |
Miki nakao, Sayo Iwanaga, Takahiro Nagasawa, Tomonori Somamoto, PHYLOGENETIC INFERENCE ON FUNCTIONS OF THE CLASSICAL COMPLEMENT PATHWAY IN BONY FISH, 14th congress of International Society of Developmental and Comparative Immunology, 2018.06. |
| 4. |
長澤 貴宏, 杣本智軌, 中尾 実樹, Ligand recognition specificity of carp thrombocytes as the natural type I IFN producing cells., The JSFS 85th Anniversary-Commemorative International Symposium「Fisheries Science for Future Generations, 2017.09. |
| 5. |
長澤 貴宏, 杣本 智軌, 中尾 実樹, Phagocytosis by carp thrombocyte needs activation factors secreted from other leukocytes population, 13th congress of International Society of Developmental and Comparative Immunology, 2015.06. |
| 6. |
長澤 貴宏, 中尾 実樹, 杣本 智軌, 中易 千早, Aja M. Rieger, Daniel R. Barreda, Phagocytosis and bactericidal abilities of teleost thrombocytes., 12th congress of International Society of Developmental and Comparative Immunology, 2012.06. |
所属学会名
板鰓類研究会
日本水産学会
国際比較免疫学会
日本比較免疫学会
学会大会・会議・シンポジウム等における役割
2017.03～2017.03, H29年度日本水産学会春季大会, 座長（Chairmanship）.
2016.08～2016.08, H28年度日本比較免疫学会, 座長（Chairmanship）.
学術論文等の審査
| 年度 |
外国語雑誌査読論文数 |
日本語雑誌査読論文数 |
国際会議録査読論文数 |
国内会議録査読論文数 |
合計 |
| 2019年度 |
2 |
|
|
|
2 |
| 2018年度 |
2 |
|
|
|
2 |
| 2017年度 |
1 |
|
|
|
1 |
| 2016年度 |
2 |
|
|
|
2 |
海外渡航状況, 海外での教育研究歴
University of Alberta, Canada, 2012.01～2012.02.
The University of Pennsylvania, UnitedStatesofAmerica, 2012.02～2012.02.
Universidad de Las Palmas de Gran Canaria, Spain, 2019.06～2019.06.
James Cook University, Australia, 2016.03～2016.03.
University of California, Davis, UnitedStatesofAmerica, 2017.02～2017.02.
第七回ユニーク会最優秀ポスター賞, 2022.09.
Best Junior Presentation Award -First Prize, International Society of Developmental and Comparative Immunology, 2012.07.
科学研究費補助金の採択状況(文部科学省、日本学術振興会)
2021年度～2023年度, 若手研究, 代表, 魚類体表粘膜創傷時における修復機構の解明.
2017年度～2019年度, 若手研究(B), 代表, 補体活性を抑制した急性ショック・炎症フリー魚の作出.
2016年度～2018年度, 基盤研究(B), 分担, 補体のホメオスタシス機能を活用した魚類粘膜の自然免疫バリアの強化.
2020年度～2020年度, 糸島市協定大学等課題解決型研究事業, 代表, メタン発酵消化液を液肥利用した場合のジャンボタニシへの影響.
2022年度, 株式会社海の中道海洋生態科学館, 研究助成金.
2018年度, 株式会社海の中道海洋生態科学館, 研究助成金.
2019年度, 株式会社海の中道海洋生態科学館, 研究助成金.
2019年度, 株式会社海の中道海洋生態科学館, 研究助成金.
2017年度～2017年度, 農学研究院若手教員支援事業(チャレンジ研究支援(Ⅰ型)), 代表, 粘膜バリアの評価系作出を目指した魚類体表粘液のプロテオーム解析.
2018年度～2018年度, 農学研究院若手教員支援事業(チャレンジ研究支援(Ⅰ型)), 代表, 低魚粉飼料給餌による魚類体表粘膜修復力低下の原因と作用機構の解明.
