Kyushu University Academic Staff Educational and Research Activities Database
List of Presentations
Fumihiro Sassa Last modified date:2020.01.26

Assistant Professor / Department of Electronics / Faculty of Information Science and Electrical Engineering

1. Yoshifumi Shimizu, Wataru Satoh, Atsushi Takashima, Fumihiro Sassa, Junji Fukuda, Hiroaki Suzuki, Microfluidic device for on-chip manipulation of liquid plugs for biosensing applications, 6th IEEE Conference on SENSORS, IEEE SENSORS 2007, 2007.12, A microfluidic device that forms a row of liquid plugs in a micro flow channel was fabricated, and its applicability to an on-chip enzyme-linked immunosorbent assay (ELISA) was demonstrated. The nanoliter liquid plugs were formed by six independent pumps and were mobilized in the micro-flow channel using a main pump. The operation of the pumps was based on the volume change caused as a result of the electrochemical production of hydrogen bubbles. When the bubbles were produced in the pumps, a polydimethylsiloxane (PDMS) diaphragm at the bottom of the compartment inflated and closed an inlet hole at the top of the reservoir. The solution in the reservoir was forced to be injected into the flow channel forming a liquid plug. After six plugs were formed, they were mobilized to a reaction chamber one by one to allow an antigen-antibody binding, cleansing, and detection. This simple and reliable liquid handling system could be applied to the detection of a tumor maker, α-fetoprotein (AFP)..
2. Zhongyuan Yang, Takaaki Soeda, Fumihiro Sassa, Kenshi Hayashi, Visualization of Spatial Distribution of on Ground Gas Sources with LSPR based 1D/2D High Speed Gas Sensor Robot System, 17th IEEE SENSORS Conference, SENSORS 2018, 2018.12, Various odor robots have been developed for gas source localization in plume with specific algorithm. However, sensor response speed is now a major limit for the promotion of odor robot using chemical substances information. In this research, we have fabricated a Localized surface plasmon resonance based gas sensor which can show a quick response to gas molecular at a high speed of above 25Hz. The sensor was mounted on a mobile robot for gas sources detection successfully. Furthermore, a two-dimensional gas sensor based on the same technique was developed for visualization of spatial distribution of gas flow..
3. Fumihiro Sassa, Kenshi Hayashi, Flexible Thermal Actuator Film for Monolithic Soft Micro Robot Process, 17th IEEE SENSORS Conference, SENSORS 2018, 2018.12, Patternable actuator film for monolithic soft micro robot process was developed. The film is consisted from thermal bimorph actuation layer and extra functional layer including driving heater layer and elastic mechanical buffer layer. A cantilever arm actuator fabricated by patterning the film was operated with 2 V applied voltage. Multi actuator device or micro robot can be fabricated without assembling process by appropriate patterning process with this film..
4. Wu Shuanghong, Fumihiro Sassa, Kenshi Hayashi, An Odor Visualization Film Based on Multi Colors Fluorescent Microbeads and Single Color Fluorescent Multi Microbeads, 17th IEEE SENSORS Conference, SENSORS 2018, 2018.12, The microbeads shape sensor film was developed in this work combines fluorescent dye agents whose fluorescent intensity can be influenced in touch of a specific odor, and Molecular Imprinting Polymer. Like photographic films, the fluorescent dye microbeads film can visualize the odor gas diffusion with the CCD camera. To enhance the fluorescence intensity of Quinine Sulfate increases in the water environment, hydrogel was applied in the film. For developing multi-odor sensors, we made four kinds of fluorescent microbeads to test whether the interaction among fluorescent dyes happened. It turned out microbeads structure successfully remained characters of every fluorescent dye..
5. Hiro Taka Yoshioka, Shota Ueno, Fumihiro Sassa, Kenshi Hayashi, Odorants measurement using hyperspectral imaging and PVC film including multi fluorescent probes, 16th IEEE SENSORS Conference, ICSENS 2017, 2017.12, Odor imaging sensor was developed with odorants sensing mechanism based on the complicated fluorescence interactions between the multi-fluorescent-probes and odorants. In this research, poly vinyl chloride (PVC) films including various fluorescent probe were developed, and fluorescence properties of the film were investigated. Using the multi fluorescent probe film, fluorescence intensity change spectrum to various odorants was measured, and odorants could be clustered by their chemical structure by multi-variate analyses of hyper spectra images..
6. 篠原 翔, 佐々 文洋, 林 健司, Gas Responding Chemiresistor with High Selectivity Using Molecularly Imprinted Polymer Composite, 平成28年度応用物理学会九州支部学術講演会, 2016.12.
7. Masashi Watanabe, Fumihiro Sassa, Kenshi Hayashi, Formation of Oriented Metal Nanostructures by Polarized Light Irradiation for Optical Sensing , IEEE SENSORS 2016, 2016.11.
8. Sho Shinohara, Fumihiro Sassa, Kenshi Hayashi, Gas Selective Chemiresistor Composed of Molecularly Imprinted Polymer Composit Ink, IEEE SENSORS 2016, 2016.11.
9. Satoshi Araki, Masashi Watanabe, Fumihiro Sassa, Kenshi Hayashi, Raman Enhanced Structure with Reconfigured Molecularly-Imprinted-Polymer for Gas Deteciton, IEEE SENSORS 2016, 2016.10.
10. Satoshi Araki, Masashi Watanabe, Fumihiro Sassa, Kenshi Hayashi, AuNPs-MIP composite SERS substrate for selective gas sensing, Asia-Pacific Conference of Transducers and Micro-Nano Technology 2016(APCOT2016), 2016.06.
11. Fumihiro Sassa, Kenshi Hayashi, Hiroaki Suzuki, Programmable droplet processing device for bio/chemical analysis, EMN meeting Droplets2016, 2016.05, Micro Total Analysis system (μ-TAS) which is consisted from micro components such as micropump, micro chemical sensor, and microflowchannel is a small chemical analysis device. [1] It can be installed into many place, due to its small size and can be promising technology for many purposes such as environmental monitoring or preventive health care. Most of μTAS are using continuous flow system, to mix chemical reagents and samples at its microflow channel network. With this way it is difficult to done several different method chemical analysis with one chip.
We developed programmable μTAS which can be carried out many analysis procedure by using droplets in micro flow channel. This device are consisted from three components. First, we developed a unit operations of droplet handling for mixing reagents. The device is consisted from flow channel with a T-junction and two micro pumps. [2] Fig.1(1) shows the droplet exchange sequence. Programmable mixing procedures were done by combination of unit operations which were shown in Fig.2 in this device. Secondly, micropump to mobilize droplet and carrier gas is developed. [3] The pump structure was shown in Fig.1(2). Elastic tubing were inserted into patterned shape memory alloy (SMA) sheet. The sheet was connected to the computer controlled electric power source to elevate the temperature by joule heating. Two unit of this SMA-elastic tube structure were connected in series to make peristaltic pump. Then, we developed electrochemical sensor for droplet detection. [4] Coulometry is a promising way for detection of small volume sample. This method require accurate volume measurement to detection accuracy. We made droplet volume measure microfluidic structure which is using surface tension of droplet shown in Fig.3(3).The accuracy of volume measurements by this structure is 2 % in relative standard deviation at 200 nl droplet. Finally, we integrated those three components to a chip. This device can be carried out programmable chemical mixing and coulometric detection by computer control, although the weight of the chip including all the components was only 1.5 g..
12. T. Sakata, K. Yamaguchi, N. Nemoto, M. Usui, Fumihiro Sassa, K. Ono, K. Takagahara, K. Kuwabara, J. Kodate, Y. Jin, Elimination of curvature in microelectromechanical-system membrane, 16th International Symposium on Semiconductor-on-Insulator Technology and Related Physics - 223rd ECS Meeting, 2013.10, This paper describes the correlation between microelectromechanical-system (MEMS) membrane curvature and implanted argon in a microelectromechanical-system membrane. When a free-standing silicon membrane, fabricated through ashing of an organic film, is cleaned by exposure to argon plasma to remove the oxidized surface, the membrane is curved uniformly. The curvature is released by annealing. Total-reflection X-ray fluorescence analysis before/after annealing reveals that argon, which is implanted into the crystal lattice of silicon by argon plasma exposure, is desorbed by annealing. This analysis also indicates that there is a linear correlation between the curvature change and implanted argon..
13. K. Ikemoto, K. Kojima, Fumihiro Sassa, M. Yokokawa, H. Suzuki, Enhancement of sensitivity of coulometric detection usng a one-electrode system, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, 2011, Coulometric analysis is effective in the analysis of an analyte in a liquid plug of a very small volume. However, in the conventional coulometric detection, it took time to collect analyte molecules dissolved in the solution. To solve this problem, we used a novel method to achieve significant enhancement of sensitivity by using a one-electrode system. As a model analyte, hydrogen peroxide was oxidized in a flow channel and silver was deposited simultaneously on another electrode in another flow channel. Coulometric detection of the deposited silver showed a step change of generated charge. As a result, measurement time was reduced significantly (less than 2 s), although conventional methods took more than 60 s to achieve the same sensitivity. This shorter measurement time also suppressed the influence of background noise, resulting in significant lowering of the detection limit..
14. X. L. Qiu, Fumihiro Sassa, D. Itoh, T. Satake, H. Suzuki, Micro titration device and its application to rice freshness measurement, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, 2011, A plug-based on-chip titration device, constructed with a glass substrate and a poly (dimethylsiloxane) (PDMS) substrate, was used for acid-base titration. The device features a volume-regulation unit to produce plugs of a uniform volume used for titration. Although the volume of plugs formed by only injecting a solution and air simultaneously from a T-junction distributed in a wide range, the plug volume became markedly uniform using the volume-regulation unit. Acid-base titration could be conducted using this device. Furthermore, freshness of rice was evaluated by titrating fatty acid in a rice grain with a KOH solution using phenolphthalein as a pH-indicator. Rice samples harvested in different years and from different areas as well as of different species were analyzed using this device. Difference was observed clearly in the titration curves. New rice showed a color change at an early stage, whereas the color change delayed with aged rice..
15. Fumihiro Sassa, Y. Al-Zain, T. Ginoza, J. Fukuda, S. Miyazaki, H. Suzuki, Programable microfluidic processor with pumping and coulometric detecting functions, 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11, 2011, An integrated microfluidic chip to handle liquid plugs of the nL order was fabricated. The chip consisted of a T-junction, shape memory alloy (SMA) micro plumps, and an electrochemical sensor. The weight of the chip including all the components was only 1.5 g. The device was operated by a computer control system connected to the chip. Driving voltage was less than 5 V. In the detection of an analyte using this chip, the volume of a sample solution was precisely measured at the T-junction, which was then separated into a liquid plug. The plug was subsequently transported to the electrochemical sensor. As a demonstration, the concentration of hydrogen peroxide was measured by coulometry. The measured charge was clearly dependent on the concentration of hydrogen peroxide..
16. Fumihiro Sassa, H. Laghzali, J. Fukuda, H. Suzuki, Coulometric detection of an analyte in a liquid plug formed in a microflow channel, TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems, 2009, Applicability of coulometric detection was tested for the analysis of an analyte in a liquid plug confined in a PDMS microflow channel. A plug prepared in the flow channel was placed on a three-electrode system and hydrogen peroxide was detected. With plugs of a small volume, the depletion of the analyte was rapid, making reproducible amperometric detection difficult particularly when the flow channel was shallow. On the other hand, coulometric detection showed a clear relationship between the generated charge and the concentration. However, when the volume of the plug was large, only a part of the analyte was used for detection, suggesting the possibility to improve the detection sensitivity further. The device was also tested in the detection of L-glutamate..