九州大学 研究者情報
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基本情報 研究活動 教育活動 社会活動
謝 家暉(ツエ カーフアイ) データ更新日:2021.10.25



主な研究テーマ
疾病模型と機能
キーワード:疾病模型; 疾病機能
2013.09.
発生毒理
キーワード:発生生物学
2013.09.
浸透圧調節
キーワード:魚類生理学
2006.09.
従事しているプロジェクト研究
Developmental toxicity
2012.01, 代表者:William KF TSE.
Fish Osmoregulation
2006.01, 代表者:William KF TSE.
Disease model and mechanism
2012.01, 代表者:William KF TSE.
Developmental functions of deubiquitylating enzyme
2009.01, 代表者:William KF TSE.
研究業績
主要著書
主要原著論文
1. Li, Rong; Huang, Chen; Ho, Jeff Cheuk Hin; Leung, Cherry Chi Tim; Kong, Richard Yuen Chong; Li, Yu; Liang, Xiao; Lai, Keng Po; Tse, William Ka Fai, The use of glutathione to reduce oxidative stress status and its potential for modifying the extracellular matrix organization in cleft lip, FREE RADICAL BIOLOGY AND MEDICINE, 10.1016/j.freeradbiomed.2020.12.455, 164, 130-138, 2021.02, OBJECTIVE:
Cleft lip (CL) is a common congenital anomaly that can be syndromic or non-syndromic. It can be triggered by the mutation of gene or environmental factors. The incidence of CL is about 1 out of 700 live births. Facial development is a complex process, and there is no existing therapy to prevent the disease development. One of the characteristics in this facial malformation is the increased presence of reactive oxygen species (ROS). In this study, we hypothesize that the antioxidant glutathione (GSH) could help to attenuate the oxidative stress in this disease.
METHODS:
Bioinformatics network pharmacology was applied to determine pharmacological targets and molecular mechanisms of GSH treatment for CL. Moreover, RNA-sequencing of the POLR1C knockdown osteoblast CL model was applied to validate the in silico data of using GSH in CL.
RESULTS:
Twenty-two core targets of GSH and CL were identified via various bioinformatics tools. The GO and KEGG analysis indicated that GSH could modulate two major families (matrix metalloproteinase and integrins), which are related to extracellular matrix modification and composition for facial development in CL. The findings from POLR1C knockdown model further supported the rescue response of GSH in CL.
CONCLUSIONS:
The study uncovered the possible pharmacological mechanism of GSH for treating CL. The data helps research group to focus on the specific pathways for understanding the biological action of GSH for treating the CL in the future..
2. Lai, Keng Po; Lin, Xiao; Tam, Nathan; Ho, Jeff Cheuk Hin; Wong, Marty Kwok-Shing; Gu, Jie; Chan, Ting Fung; Tse, William Ka Fai, Osmotic stress induces gut microbiota community shift in fish, ENVIRONMENTAL MICROBIOLOGY, 10.1111/1462-2920.15150, 22, 9, 3784-3802, 2020.09, [URL], Alteration of the gut microbiota plays an important role in animal health and metabolic diseases. However, little is known with respect to the influence of environmental osmolality on the gut microbial community. The aim of the current study was to determine whether the reduction in salinity affects the gut microbiota and identify its potential role in salinity acclimation. Using Oryzias melastigma as a model organism to perform progressive hypotonic transfer experiments, we evaluated three conditions: seawater control (SW), SW to 50% sea water transfer (SFW) and SW to SFW to freshwater transfer (FW). Our results showed that the SFW and FW transfer groups contained higher operational taxonomic unit microbiota diversities. The dominant bacteria in all conditions constituted the phylum Proteobacteria, with the majority in the SW and SFW transfer gut comprising Vibrio at the genus level, whereas this population was replaced by Pseudomonas in the FW transfer gut. Furthermore, our data revealed that the FW transfer gut microbiota exhibited a reduced renin–angiotensin system, which is important in SW acclimation. In addition, induced detoxification and immune mechanisms were found in the FW transfer gut microbiota. The shift of the bacteria community in different osmolality environments indicated possible roles of bacteria in facilitating host acclimation..
3. Marco Chi Chung Lau, Ernest Man Lok Kwong, Keng Po Lai, Jing Woei Li, Jeff Cheuk Hin Ho, Ting Fung Chan, Chris Kong Chu Wong, Yun Jin Jiang, Ka Fai William Tse, Pathogenesis of POLR1C-dependent Type 3 Treacher Collins Syndrome revealed by a zebrafish model, Biochimica et Biophysica Acta - Molecular Basis of Disease, 10.1016/j.bbadis.2016.03.005, 1862, 6, 1147-1158, 2016.06, [URL], Treacher Collins Syndrome (TCS) is a rare congenital birth disorder (1 in 50,000 live births) characterized by severe craniofacial defects, including the downward slanting palpebral fissures, hypoplasia of the facial bones, and cleft palate (CP). Over 90% of patients with TCS have a mutation in the TCOF1 gene. However, some patients exhibit mutations in two new causative genes, POLR1C and POLR1D, which encode subunits of RNA polymerases I and III, that affect ribosome biogenesis. In this study, we examine the role of POLR1C in TCS using zebrafish as a model system. Our data confirmed that polr1c is highly expressed in the facial region, and dysfunction of this gene by knockdown or knock-out resulted in mis-expression of neural crest cells during early development that leads to TCS phenotype. Next generation sequencing and bioinformatics analysis of the polr1c mutants further demonstrated the up-regulated p53 pathway and predicted skeletal disorders. Lastly, we partially rescued the TCS facial phenotype in the background of p53 mutants, which supported the hypothesis that POLR1C-dependent type 3 TCS is associated with the p53 pathway..
主要学会発表等
学会活動
所属学会名
Japanese Society of Developmental Biology
Hong Kong Society for Developmental Biology
The Asia-Pacific Developmental Biology Network
学会大会・会議・シンポジウム等における役割
2020.05.19~2020.05.22, The 53rd Annual Meeting of the Japanese Society of Developmental Biologists, Chairperson of the Mini-Symposium 1: Developmental Toxicology and Toxicogenomics.
学会誌・雑誌・著書の編集への参加状況
2021.10, Frontiers in Endocrinology, 国際, 編集委員.
2021.08, Frontiers in Public Health, 国際, 編集委員.
2021.02, Frontiers in Physiology, 国際, 編集委員.
2020.08, Translational Oncology, 国際, 査読委員.
2018.05, BMC Genomics, 国際, 編集委員.
2019.10~2021.08, Antibiotics, 国際, 編集委員.
学術論文等の審査
年度 外国語雑誌査読論文数 日本語雑誌査読論文数 国際会議録査読論文数 国内会議録査読論文数 合計
2010年度      
2011年度      
2013年度      
2015年度      
2016年度 14        14 
2017年度 11        11 
2018年度 24        24 
2019年度 26        26 
2020年度 34        34 
その他の研究活動
海外渡航状況, 海外での教育研究歴
City University of Hong Kong, Hong Kong , 2019.04~2019.04.
National Health Research Institutes, Taiwan, 2019.03~2019.03.
National Health Research Institutes, Taiwan, 2018.08~2018.08.
National Health Research Institutes, Taiwan, 2018.06~2018.07.
City University of Hong Kong, Hong Kong , 2018.03~2018.03.
National Health Research Institutes, Taiwan, 2018.02~2018.03.
National Health Research Institutes, Taiwan, 2017.12~2017.12.
City University of Hong Kong, Hong Kong , 2017.11~2017.11.
National Health Research Institutes, Taiwan, 2017.08~2017.08.
National Health Research Institutes , Taiwan, 2017.06~2017.06.
City University of Hong Kong, Hong Kong , 2017.02~2017.03.
National Health Research Institutes, Taiwan, 2016.08~2016.08.
Hobg Kong Baptist University, Hong Kong , 2016.09~2016.09.
Beijing Normal University-Hong Kong Baptist University, United International College, China, 2015.02~2015.06.
National Health Research Institutes, Taiwan, 2015.08~2015.08.
National Health Research Institutes, Taiwan, 2014.08~2014.08.
National Health Research Institutes, Taiwan, 2013.08~2013.08.
Hong Kong Baptist University, Hong Kong , 2012.06~2016.02.
Massachusetts General Hospital, Harvard Medical School, UnitedStatesofAmerica, 2011.09~2012.06.
Institute of Molecular and Cell Biology, A*STAR, Singapore, 2007.11~2009.09.
外国人研究者等の受入れ状況
2019.04~2019.04, 2週間以上1ヶ月未満, Saarland University, Germany.
受賞
Postgraduate studentship, Hong Kong Baptist University, 2004.09.
Student Travel Award, 7th International Congress on the Biology of Fish, 2006.01.
APDBN Travel Award, The Asia-Pacific Developmental Biology Network (APDBN), 2009.05.
JSPS Postdoctoral Fellowship, The Japan Society for the Promotion of Science (JSPS), 2009.09.
研究資金
科学研究費補助金の採択状況(文部科学省、日本学術振興会)
2009年度~2011年度, 特別研究員奨励費, Osmoregulation and Osmosensing mechanisms in eel mitochondria-rich cells.
日本学術振興会への採択状況(科学研究費補助金以外)
2017年度~2019年度, 二国間交流, 代表, The potential use of antioxidant to treat the POLR1C-dependent Type 3 Treacher Collins Syndrome..
競争的資金(受託研究を含む)の採択状況
2022年度~2024年度, Alliance of International Science Organizations (ANSO): International Research Collaboration, 分担, The impact of microplastics on brain health and reproductive system health and the damage of barrier function.
2020年度~2022年度, Department of Science and Technology of Guangdong Province: International Science and Technology Collaboration, 分担, Effects of plastic microparticles and their environmental pollutants enrichment on reproductive ability of parents and offspring.
2018年度~2019年度, 公益財団法人クリタ水・環境科学振興財団, 代表, 水環境の金属酸化物ナノ粒子の発生毒性影響評価.
2017年度~2018年度, 公益財団法人クリタ水・環境科学振興財団/萌芽的研究 , 代表, ゼブラフィッシュ胚を使用した水質検査ガイドラインの確立/To establish the guideline of using zebrafish embryos to monitor the water quality.
2015年度~2016年度, National Natural Science Foundation of China (NSFC), CHINA., 代表, Role of deubiquitylating enzyme, cops6, in zebrafish craniofacial palatogenesis..
共同研究、受託研究(競争的資金を除く)の受入状況
2020.04~2022.03, 代表, The osmoregulatory mechanism in medaka .
2020.04~2022.03, 代表, The role of polr1c in regulating endodermal cells in Type 3 Treacher Collins syndrome.
2019.04~2020.03, 代表, The role of polr1c in regulating endodermal cells in Type 3 Treacher Collins syndrome.
2019.04~2021.03, 代表, Triclosan: a risk factor of fatty liver disease.
2019.04~2020.03, 代表, The osmoregulatory roles of Ostf1b in medaka.
2018.04~2019.03, 代表, Triclosan: a risk factor of fatty liver disease.
2018.04~2019.03, 代表, The osmoregulatory roles of Ostf1b in medaka.
2018.04~2019.03, 代表, Generation of osmotic stress transcription factor 1b knockout medaka for fish osmoregulation studies.
2017.04~2018.03, 代表, Generation of knockout medaka for fish osmoregulation studies.
2015.04~2016.03, 代表, Screening for transgenic craniofacial developmental model for live-imaging..
学内資金・基金等への採択状況
2018年度~2019年度, Challenging Research Type II
Faculty of Agriculture, Kyushu University, 代表, The emerging craniofacial developmental roles of polr1c in Treacher Collins Syndrome.
2014年度~2016年度, Dr. Lee Shau Kee’s donation fund, Hong Kong Baptist University., 分担, To Define the Effects of Endocrine-Disrupting Chemicals on Gonadal Sex Development..
2013年度~2016年度, Hong Kong Baptist University. , 代表, Start-up.
2013年度~2013年度, Science Faculty Research Fund, Hong Kong Baptist University. , 代表, The Characterization of Novel mib Alleles..
2014年度~2014年度, Science Faculty Research Fund, Hong Kong Baptist University. , 代表, Functions of Deubiquitylating Enzymes on Craniofacial Development. .
2014年度~2014年度, Science Faculty Research Fund, Hong Kong Baptist University. , 代表, Functional Studies of polr1c in Facial Development. .
2015年度~2015年度, Science Faculty Research Fund, Hong Kong Baptist University. , 代表, Characterization of cpt1a in liver development and the generation of cpt1a transgenic zebrafish for environmental toxicology screening..
2015年度~2015年度, Science Faculty Research Fund, Hong Kong Baptist University., 代表, The Role of DUBs in Cancer Metastasis..

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