|松岡 悠太（まつおか ゆうた）||データ更新日：2021.11.12|
|1.||Matsuoka Y, Yamada KI, Detection and structural analysis of lipid-derived radicals in vitro and in vivo, Free Radic Res, 10.1080/10715762.2021.1881500, 8, 1-9, 2021.02, [URL], Lipids can be oxidized by reactive oxygen species, resulting in lipid peroxidation and the formation of reactive metabolites such as lipid-derived electrophiles. These products have been reported to induce inflammation, angiogenesis, and ferroptosis. Lipid peroxidation can produce many different products, each of which performs a different function, and which can be challenging to detect in vivo. The initial products of lipid oxidation are lipid-derived radicals, which can cause extensive chain reactions leading to lipid peroxidation. Hence, the ability to detect lipid radicals may provide information about this important class of molecules and the mechanism by which they cause cellular and tissue damage in a wide range of oxidative conditions. In this review, we report recent scientific advances in the detection of lipid-derived radicals in vitro and in cultured cells. We also introduce the possibility of visualization and structural analysis of lipid-derived radicals generated not only in in cells but also in animal tissue samples from oxidative disease models, using fluorescence-based lipid radicals' detection probes. We anticipate that the various innovative techniques summarized in this paper will be applied and further developed to clarify the role of lipid peroxidation in the pathogenesis of oxidative stress-associated diseases..|
|2.||Matsuoka Y, Izumi Y, Takahashi M, Bamba T, Yamada KI., Method for Structural Determination of Lipid-Derived Radicals, American Chemical Society, 10.1021/acs.analchem.0c00053, 19, 92(10), 6993-7002, 2020.05, [URL], Diversified oxidized-lipid molecules are responsible for inflammation and cell death, including ferroptosis. Lipid radicals are the source of these oxidized lipids, which are the initial key molecules in the lipid peroxidation chain reaction. However, owing to their extremely high reactivity and short half-life, an established detection technique is not available. Here, we propose a high-performance liquid chromatography fluorometry and high-resolution tandem mass spectrometry system combined with a fluorescent probe as a structural analysis method for lipid-derived radicals. We detected 132 lipid-derived radicals, including 111 new species, from five polyunsaturated fatty acids. In addition, a database was constructed for which the initial fatty acid could be determined using the radical structure. Further, 12 endogenous lipid-derived radicals were identified in carcinogen-induced liver cancer mouse models. Therefore, this method and its corresponding database will provide novel insights into mechanisms underlying the lipid peroxidation, including the associated inflammation and ferroptosis..|
|3.||Matsuoka Y, Ohkubo K, Yamasaki T, Yamato M, Ohtabu H, Shirouzu T, Fukuzumi S, Yamada KI, A profluorescent nitroxide probe for ascorbic acid detection and its application to quantitative analysis of diabetic rat plasma, RSC Advances, 10.1039/C6RA07693A, 60907-60915, 2016.06, [URL].|
|4.||Yamada KI, Mito F, Matsuoka Y, Ide S, Shikimachi K, Fujiki A, Kusakabe D, Ishida Y, Enoki M, Tada A, Ariyoshi M, Yamasaki T, Yamato M, Fluorescence probes to detect lipid-derived radicals, Nature Chemical Biology, 10.1038/nchembio.2105, 12, 608-613, 2016.04, [URL], Lipids and their metabolites are easily oxidized in chain reactions initiated by lipid radicals, forming lipid peroxidation products that include the electrophiles 4-hydroxynonenal and malondialdehyde. These markers can bind cellular macromolecules, causing inflammation, apoptosis and other damage. Methods to detect and neutralize the initiating radicals would provide insights into disease mechanisms and new therapeutic approaches. We describe the first high-sensitivity, specific fluorescence probe for lipid radicals, 2,2,6-trimethyl-4-(4-nitrobenzo[1,2,5]oxadiazol-7-ylamino)-6-pentylpiperidine-1-oxyl (NBD-Pen). NBD-Pen directly detected lipid radicals in living cells by turn-on fluorescence. In a rat model of hepatic carcinoma induced by diethylnitrosamine (DEN), NBD-Pen detected lipid radical generation within 1 h of DEN administration. The lipid radical scavenging moiety of NBD-Pen decreased inflammation, apoptosis and oxidative stress markers at 24 h after DEN, and liver tumor development at 12 weeks. Thus, we have developed a novel fluorescence probe that provides imaging information about lipid radical generation and potential therapeutic benefits in vivo..|
|5.||Matsuoka Y, Yamato M, Yamada KI, Fluorescence probe for the convenient and sensitive detection of ascorbic acid, Journal of Clinical Biochemistry and Nutrition, 10.3164/jcbn.15-105, 58, 16-22, 2016.01, [URL].|
Society for Redox Biology and Medicine
学術奨励賞, 日本薬学会 九州山口支部, 2020.11.
奨励賞, 電子スピンサイエンス学会2020, 2020.11.
星野賞・研究奨励賞, 第29回バイオメディカル分析科学シンポジウム, 2016.09.
最優秀発表賞, 第68回日本酸化ストレス学会学術集会, 2015.06.
SEST Excellent Presentation Award, APES2014 IES SEST 2014 (The 53rd SEST Annual Meeting),, 2014.11.
年会優秀発表賞, 日本薬学会第134年会, 2014.03.
年会優秀発表賞, 日本薬学会第133年会, 2013.03.
年会優秀発表賞・講演ハイライト採択, 日本薬学会第132年会, 2012.03.
優秀発表賞, 第28回日本薬学会九州支部会, 2011.12.
2021年度～2022年度, 若手研究, 代表, 生体内酸化リン脂質の包括的解析技術の開発.
2018年度～2019年度, 若手研究(A), 代表, 脂質アルデヒドの体系的プロファイリングシステムの構築およびその応用.
2016年度～2017年度, 若手研究(スタートアップ), 代表, 生体内脂質ラジカルの構造解析法の開発とその応用.