Kyushu University Academic Staff Educational and Research Activities Database
List of Presentations
Takahashi Tatsuro Last modified date:2022.06.01

Professor / Integrative Biology / Department of Biology / Faculty of Sciences

1. Tatsuro Takahashi, Yoshitaka Kawasoe, Satomi Oda, Aya Sakazume, Regulation of the fidelity of homology-directed repair in Xenopus egg extracts, The 11th quinquennial conference on DNA repair, 2022.03, The mismatch repair (MMR) system protects genetic information by handling mispairs arising from DNA replication errors and homology-directed repair between divergent sequences. Replication errors are corrected by the MMR system in a strand-specific manner to restore original genetic information. In contrast, homology-directed repair between divergent sequences is suppressed through the unwinding of intermediates. Genetic studies in yeast have shown that this process, called anti-recombination or heteroduplex rejection, depends on the Msh2-Msh6 (MutSα) mismatch recognition complex and RecQ homolog DNA helicase Sgs1. However, mechanistic details and the regulation of anti-recombination remain still ambiguous, especially in vertebrates, partly due to the insufficiency of in vitro model systems.
In this study, we set up a single-strand annealing (SSA) model system in Xenopus egg extracts and found that sequence divergence between two repeating units significantly delays the annealing reaction and reduces the efficiency of SSA. Immunodepletion experiments showed that this reduction of SSA is mediated by MutSα and the Werner helicase. MutSα and the Werner helicase were also important for the fidelity of SSA. We will discuss possible mechanisms and regulations of how these factors increase the SSA fidelity..
2. Tatsuro Takahashi, The quality control mechanism of homology-directed repair in Xenopus egg extracts, 第43回日本分子生物学会年会, 2020.12.
3. Tatsuro Takahashi, The quality control mechanism of homology-directed repair in Xenopus egg extracts, CHROMOSOME DYNAMICS, An international symposium on chromatin and chromosome stability, 2019.12.
4. Riki Terui, Tatsuro Takahashi, Reconstitution of a chromatin-remodeling reaction that facilitates post-replicative DNA mismatch repair, 第42回日本分子生物学会年会, 2019.12.