||Axonal Wiring Specificity by Differential cAMP Levels in the Mouse Olfactory System (GE & Science Prize winning essay)..
||Deep-Tissue Imaging Using Optical Clearing Agents.
||Satoshi Fujimoto, Marcus N. Leiwe, Yuko Muroyama, Reiko Kobayakawa, Ko Kobayakawa, Tetsuichiro Saito, Takeshi Imai, Spontaneous activity governs the dendrite pruning of mitral cells to establish discrete connectivity, CHEMICAL SENSES, Vol.41, No.9, p.E146, 2016.11.
||Ryo Iwata, Takeshi Imai, Sampling mode- and concentration-invariant temporal odor coding by airflow-driven neuronal oscillations, CHEMICAL SENSES, Vol.41, No.9, p.E143, 2016.11.
||SeeDB2: A tissue clearing method for high-resolution fluorescence imaging.
||Satoshi Fujimoto, Marcus N Leiwe, Richi Sakaguchi, Yuko Muroyama, Reiko Kobayakawa, Ko Kobayakawa, Tetsuichiro Saito, Takeshi Imai, Spontaneous activity generated within the olfactory bulb establishes the discrete wiring of mitral cell dendrites, bioRxiv, 10.1101/625616, p.625616, 2019.05.
||BMPR-2 gates activity-dependent stabilization of dendrites during mitral cell remodeling
SUMMARYDeveloping neurons initially form excessive neurites and then remodel them based on molecular cues and neuronal activity. Developing mitral cells in the olfactory bulb initially extend multiple primary dendrites. They then stabilize single primary dendrites, while eliminating others. However, the mechanisms underlying the selective dendrite remodeling remain elusive. Using CRISPR/Cas9-based knockout screening combined with in utero electroporation, we identified BMPR-2 as a key regulator for the selective dendrite stabilization. Bmpr2 knockout and its rescue experiments show that BMPR-2 inhibits LIMK without ligands and thereby facilitates dendrite destabilization. In contrast, the overexpression of antagonists and agonists indicate that ligand-bound BMPR-2 stabilizes dendrites, most likely by releasing LIMK. Using genetic and FRET imaging experiments, we also demonstrate that free LIMK is activated by NMDARs via Rac1, facilitating dendrite stabilization through F-actin formation. Thus, the selective stabilization of mitral cell dendrites is ensured by concomitant inputs of BMP ligands and neuronal activity..
||Ke M-T, Imai T, Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2, bio-protocol , 10.21769/BioProtoc, 2018.10.