Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
PANKAJ ATTRI Last modified date:2021.11.18

Associate Professor / Center of Plasma Nono-interface Engineering

1. Pankaj Attri, Jae-Hyun Park, Joey De Backer, Myeongkyu Kim, Ji-Hye Yun, Yunseok Heo, Sylvia Dewilde, Masaharu Shiratani, Eun Ha Choi, Weontae Lee, Annemie Bogaerts, Structural modification of NADPH oxidase activator (Noxa 1) by oxidative stress
An experimental and computational study, International Journal of Biological Macromolecules, 10.1016/j.ijbiomac.2020.09.120, 163, 2405-2414, 2020.09, NADPH oxidases 1 (NOX1) derived reactive oxygen species (ROS) play an important role in the progression of cancer through signaling pathways. Therefore, in this paper, we demonstrate the effect of cold atmospheric plasma (CAP) on the structural changes of Noxa1 SH3 protein, one of the regulatory subunits of NOX1. For this purpose, firstly we purified the Noxa1 SH3 protein and analyzed the structure using X-ray crystallography, and subsequently, we treated the protein with two types of CAP reactors such as pulsed dielectric barrier discharge (DBD) and Soft Jet for different time intervals. The structural deformation of Noxa1 SH3 protein was analyzed by various experimental methods (circular dichroism, fluorescence, and NMR spectroscopy) and by MD simulations. Additionally, we demonstrate the effect of CAP (DBD and Soft Jet) on the viability and expression of NOX1 in A375 cancer cells. Our results are useful to understand the structural modification/oxidation occur in protein due to reactive oxygen and nitrogen (RONS) species generated by CAP..
2. Pankaj Attri, Kenji Ishikawa, Takamasa Okumura, Kazunori Koga, Masaharu Shiratani, Plasma agriculture from laboratory to farm
A review, Processes, 10.3390/PR8081002, 8, 8, 2020.08, In recent years, non-thermal plasma (NTP) application in agriculture is rapidly increasing. Many published articles and reviews in the literature are focus on the post-harvest use of plasma in agriculture. However, the pre-harvest application of plasma still in its early stage. Therefore, in this review, we covered the effect of NTP and plasma-treated water (PTW) on seed germination and growth enhancement. Further, we will discuss the change in biochemical analysis, e.g., the variation in phytohormones, phytochemicals, and antioxidant levels of seeds after treatment with NTP and PTW. Lastly, we will address the possibility of using plasma in the actual agriculture field and prospects of this technology..
3. Pankaj Attri, Sooho Choi, Minsup Kim, Masaharu Shiratani, Art E. Cho, Weontae Lee, Influence of alkyl chain substitution of ammonium ionic liquids on the activity and stability of tobacco etch virus protease, International Journal of Biological Macromolecules, 10.1016/j.ijbiomac.2020.03.175, 155, 439-446, 2020.07, Ionic liquids (ILs) are known to provide stability to biomolecules. ILs are also widely used in the fields of chemical engineering, biological engineering, chemistry, and biochemistry because they facilitate enzyme catalyzed reactions and enhance their conversion rate. In this work, we have evaluated the influence of alkyl chain substitution of ammonium ILs such as diethylammonium dihydrogen phosphate (DEAP) and triethylammonium hydrogen phosphate (TEAP) for the stability and activity of the tobacco etch virus (TEV) protease. Further, we performed molecular dynamics (MD) simulations to calculate the RMSD (root mean square deviation) for TEV and TEV + ILs. Experimental and simulations results show that TEV is more stable in the presence of TEAP than DEAP. Whereas, TEV protease activity for the cleavage of fusion proteins is preserved in the presence of DEAP while lost in the presence of TEAP. Hence, DEAP IL can serve as alternative solvents for the stability of the TEV protease with preserved activity. To the best of our knowledge, this is first study to show that ILs can stabilize and maintain the TEV protease cleavage activity..
4. Kazunori Koga, Pankaj Attri, Kunihiro Kamataki, Naho Itagaki, Masaharu Shiratani, Vida Mildaziene, Impact of radish sprouts seeds coat color on the electron paramagnetic resonance signals after plasma treatment, Japanese Journal of Applied Physics, 10.35848/1347-4065/ab7698, 59, SHHF01, 2020.05, Recently, cold atmospheric plasma (CAP) treatment on seed germination has emerged as a useful technique to increase agriculture production, although, the mechanism of the cold plasma in seed germination is still under investigation. In this work, we studied the role of pigments in the seed coat of radish sprouts on the electron spin resonance (ESR) signals before and after CAP treatment. Radish sprouts seeds having gray color show enhanced ESR signals after the CAP treatment, whereas, no increased ESR signals were observed for brown color seeds of radish sprouts as compared to their respective control seeds. These results reveal that seeds from the same harvest year having different seed coat colors show different responses to the plasma treatment. Although ESR signal intensity can vary with the harvest year, the change in ESR signal intensity after plasma treatment depends on the seed coat color. Independently on the harvest year (2017 and 2018), CAP increased ESR signals stronger in the grey seeds in comparison to the brown ones. The results indicated that seed coat color may be an important variable for understanding differences in the extent of CAP effects on seeds..
5. Pankaj Attri, Jamoliddin Razzokov, Maksudbek Yusupov, Kazunori Koga, Masaharu Shiratani, Annemie Bogaerts, Influence of osmolytes and ionic liquids on the Bacteriorhodopsin structure in the absence and presence of oxidative stress
A combined experimental and computational study, International Journal of Biological Macromolecules, 10.1016/j.ijbiomac.2020.01.179, 148, 657-665, 2020.04, Understanding the folding and stability of membrane proteins is of great importance in protein science. Recently, osmolytes and ionic liquids (ILs) are increasingly being used as drug delivery systems in the biopharmaceutical industry. However, the stability of membrane proteins in the presence of osmolytes and ILs is not yet fully understood. Besides, the effect of oxidative stress on membrane proteins with osmolytes or ILs has not been investigated. Therefore, we studied the influence of osmolytes and ILs as co-solvents on the stability of a model membrane protein (i.e., Bacteriorhodopsin in purple membrane of Halobacterium salinarum), using UV–Vis spectroscopy and molecular dynamics (MD) simulations. The MD simulations allowed us to determine the flexibility and solvent accessible surface area (SASA) of Bacteriorhodopsin protein in the presence and/or absence of co-solvents, as well as to carry out principal component analysis (PCA) to identify the most important movements in this protein. In addition, by means of UV–Vis spectroscopy we studied the effect of oxidative stress generated by cold atmospheric plasma on the stability of Bacteriorhodopsin in the presence and/or absence of co-solvents. This study is important for a better understanding of the stability of proteins in the presence of oxidative stress..
6. Pankaj Attri, Carbon Nanotubes (CNTs): A Potential Nanomaterial for Water Purification, Journal of Composites Science, 10.3390/jcs4030135, 135, 2020, Nanomaterials such as carbon nanotubes (CNTs) have been used as an excellent material for catalysis, separation, adsorption and disinfection processes. CNTs have grabbed the attention of the scientific community and they have the potential to adsorb most of the organic compounds from water. Unlike, reverse osmosis (RO), nanofiltration (NF) and ultrafiltration (UF) membranes aligned CNT membranes can act as high-flow desalination membranes. CNTs provide a relatively safer electrode solution for biosensors. The article is of the utmost importance for the scientists and technologists working in water purification technologies to eliminate the water crisis in the future. This review summarizes about the application of CNTs in water purification..
7. Pankaj Attri and Annemie Bogaerts, Perspectives of Plasma-treated Solutions as Anticancer Drugs, Anti-Cancer Agents in Medicinal Chemistry, 10.2174/187152061904190521102345, 19, 436-438, Year: 2019
Volume: 19
First Page: 436
Last Page: 438, 2019.07, [URL].
8. B. Ghimire, E.J. Szili, P. Lamichhane, R. D. Short, J. S. Lim, Pankaj Attri, K. Masur, K.-D. Weltmann, S.-H. Hong, E. H. Choi, The role of UV photolysis and molecular transport in the generation of reactive species in a tissue model with a cold atmospheric pressure plasma jet, Appl. Phys. Lett. , 114, 093701, 2019.03, [URL].
9. Pankaj Attri, Cold Atmospheric Plasma Activated Solution: A New Approach for Cancer Treatment, Anti-Cancer Agents in Medicinal Chemistry, 18, 768, 2018.10, [URL].
10. P. Shaw, N. Kumar, H. S. Kwak, J. H. Park, H. S. Uhm, A. Bogaerts, E. H. Choi and Pankaj Attri , Bacterial inactivation by plasma treated water enhanced by reactive nitrogen species, SCIENTIFIC REPORTS, 10.1038/s41598-018-29549-6, 8, 8, 11268, 2018.07.
11. Pankaj Attri, J. Han, S. Choi, E. H. Choi, A. Bogaerts and Weontae Lee, CAP modifies the structure of a model protein from thermophilic bacteria: mechanisms of CAP-mediated inactivation, SCIENTIFIC REPORTS, 10.1038/s41598-018-28600-w, 8, 8, 10218, 2018.07.
12. N. Kumar, Pankaj Attri, S. Dewilde and A. Bogaerts, Inactivation of human pancreatic ductal adenocarcinoma with atmospheric plasma treated media and water: a comparative study, JOURNAL OF PHYSICS D-APPLIED PHYSICS, 10.1088/1361-6463/aac571, 51, 25, 51, 255401, 2018.06.
13. D. U. Lee, J. Y. Jeong, J. W. Han, G.-C. Kwon, Pankaj Attri and I. T. Kim, Effect of Ionic Liquids on the Physical Properties of the Newly Synthesized Conducting Polymer, INTERNATIONAL JOURNAL OF POLYMER SCIENCE, 10.1155/2018/8275985, 2018, Article ID 8275985, 2018.06.
14. Pankaj Attri, J. H. Park, A. Ali, E. H. Choi, How Does Plasma Activated Media Treatment Differ From Direct Cold Plasma Treatment?, ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, 10.2174/1871520618666180406121734, 18, 6, 805-814, 18, 805, 2018.05.
15. S. H. Kim, Pankaj Attri and In Tae Kim, Improvement in the diffraction efficiency of a polymer using an ionic liquid, JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 10.2298/JSC170527107K, 83, 2, 213-220, 83, 213, 2018.04.
16. N. Kumar, P. Shaw, J. Razzokov, M. Yusupov, Pankaj Attri, H. S. Uhm, E. H. Choi and A. Bogaerts, Enhancement of cellular glucose uptake by reactive species: a promising approach for diabetes therapy, RSC ADVANCES, 10.1039/c7ra13389h, 8, 18, 9887-9894, 8, 9887, 2018.03.
17. Pankaj Attri, F. Tochikubo, J. H. Park, E. H. Choi, K. Koga and M. Shiratani, Impact of Gamma rays and DBD plasma treatments on wastewater treatment, SCIENTIFIC REPORTS, 10.1038/s41598-018-21001-z, 8, 8, 2926, 2018.02.
18. Pankaj Attri, Cold atmospheric plasma activated solution
A new approach for cancer treatment, Anti-Cancer Agents in Medicinal Chemistry, 10.2174/187152061806181112124717, 18, 6, 2018.01.
19. Bharti Arora, Ji Hoon Park, Eun Ha Choi, Pankaj Attri, Sol-gel-based bioceramics
From materials to medicine, Smart Ceramics Preparation, Properties, and Applications, 10.1201/9781315163598, 431-442, 2018.01, The progress of nanotechnology has opened the path for the development of new biomaterials. Recent developments have demonstrated that sol-gel bioceramics play an important role in the biomedical field due to their superior biological and mechanical properties. A bioactive hydroxyapatite (HA) is a common bioceramic that attaches to living tissues in vivo at body temperature. Sol-gel processes are now being used to produce bioactive coatings, powders, and substrates that can facilitate control over biological behavior of proteins and cells with broad clinical applications. It has been found that HA particles can down-regulate the expression of some cancerogenic genes in 432tumor cells. The nano-HA particles from the biodegradation of HA ceramics could penetrate the membranes of tumor cells. A rapid and economic sol-gel method has been designed to synthesize sodium silicate (Na 2 SiO 3 ) into silica gel in large quantities. Sol-gel-based bioceramics have the potential to present unexpected excellent biological functions. Thus, the understanding and control of the interactions between bioceramics and biological entities may play one of the leading roles in the development of nanomedicine..
20. Pankaj Attri, M. Kim, E. H. Choi, A. E. Cho, K. Koga and M. Shiratani, Impact of an ionic liquid on protein thermodynamics in the presence of cold atmospheric plasma and gamma rays, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 10.1039/c7cp04083k, 19, 37, 25277-25288, 19, 25277, 2017.10.
21. Pankaj Attri, M. Kim, T. Sarinont, E. H. Choi, H. Seo, A. E. Cho, K. Koga and M. Shiratani, The protective action of osmolytes on the deleterious effects of gamma rays and atmospheric pressure plasma on protein conformational changes, SCIENTIFIC REPORTS, 10.1038/s41598-017-08643-1, 7, 7, 8689, 2017.08.
22. Y. Lee, Pankaj Attri, S. Kim, S. Lee, J. Kim, T. Cho and I. T. Kim, Photovoltaic properties of novel thiophene- and selenophene-based conjugated low bandgap polymers: a comparative study, NEW JOURNAL OF CHEMISTRY, 10.1039/c7nj00151g, 41, 14, 6315-6321, 41, 6315, 2017.07.
23. Pankaj Attri, J. Gaur, S. Choi, M. Kim, R. Bhatia, N. Kumar, J. H. Park, A. E. Cho, E. H. Choi and W. Lee, Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein, SCIENTIFIC REPORTS, 10.1038/s41598-017-02690-4, 7, 7, 2636, 2017.06.