マウスを用いた記憶・学習メカニズムの解明
キーワード:記憶・学習、マウス
2020.04~2024.03.
小林 曉吾(こばやし きようご) | データ更新日:2023.12.06 |
主な研究テーマ
研究業績
主要原著論文
1. | Kyogo S Kobayashi, Naoki Matsuo, Persistent representation of the environment in the hippocampus., Cell Reports, 10.1016/j.celrep.2022.111989, 42, 1, 111989-111989, 2023.01, In the hippocampus, environmental changes elicit rearrangement of active neuronal ensembles or remapping of place cells. However, it remains elusive how the brain ensures a consistent representation of a certain environment itself despite salient events occurring there. Here, we longitudinally tracked calcium dynamics of dorsal hippocampal CA1 neurons in mice subjected to contextual fear conditioning and extinction training. Overall population activities were significantly changed by fear conditioning and were responsive to footshocks and freezing. However, a small subset of neurons, termed environment cells, were consistently active in a specific environment irrespective of experiences. A decoder modeling study showed that these cells, but not place cells, were able to predict the environment to which the mouse was exposed. Environment cells might underlie the constancy of cognition for distinct environments across time and events. Additionally, our study highlights the functional heterogeneity of cells in the hippocampus.. |
2. | Kyogo Kobayashi, Shunji Nakano, Mutsuki Amano, Daisuke Tsuboi, Tomoki Nishioka, Shingo Ikeda, Genta Yokoyama, Kozo Kaibuchi, Ikue Mori, Single-Cell Memory Regulates a Neural Circuit for Sensory Behavior, Cell Reports, 10.1016/j.celrep.2015.11.064, 14, 1, 11-21, 2016.01, [URL], Unveiling the molecular and cellular mechanisms underlying memory has been a challenge for the past few decades. Although synaptic plasticity is proven to be essential for memory formation, the significance of "single-cell memory'' still remains elusive. Here, we exploited a primary culture system for the analysis of C. elegans neurons and show that a single thermosensory neuron has an ability to form, retain, and reset a temperature memory. Genetic and proteomic analyses found that the expression of the single-cell memory exhibits inter-individual variability, which is controlled by the evolutionarily conserved CaMKI/IV and Raf pathway. The variable responses of a sensory neuron influenced the neural activity of downstream interneurons, suggesting that modulation of the sensory neurons ultimately determines the behavioral output in C. elegans. Our results provide proof of single-cell memory and suggest that the individual differences in neural responses at the single-cell level can confer individuality.. |
作品・ソフトウェア・データベース等
1. | Kyogo S Kobayashi, Zenodo: MATLAB code used in Kobayashi and Matsuo, Cell Reports (2023), 2023.01 MATLAB code used in Kobayashi and Matsuo, Cell Reports (2023)., [URL]. |
2. | Kyogo S Kobayashi, GitHub: MATLAB code used in Kobayashi and Matsuo, Cell Reports (2023), 2023.01 MATLAB code used in Kobayashi & Matsuo, Cell Reports (2023), [URL]. |
学会活動
受賞
卒業論文発表会「優秀発表者賞」, 名古屋大学 理学部 生命理学科, 2009.03.
修士論文発表会「優秀発表者賞」, 名古屋大学 大学院理学研究科 生命理学専攻, 2011.03.
研究科長顕彰, 名古屋大学 大学院理学研究科, 2011.03.
博士課程教育リーディングプログラム IGER「優秀研究者賞」, 名古屋大学, 2014.01.
研究資金
科学研究費補助金の採択状況(文部科学省、日本学術振興会)
2013年度~2017年度, 基盤研究(C), 記憶情報を担う細胞集団の時空間的変化の解析.
2020年度~2023年度, 若手研究, 代表, 記憶を”あいまい”にする神経基盤の解明:記憶エングラム細胞への抑制性入力.
2017年度~2019年度, 特別研究員奨励費, 代表, 記憶の特異性を制御する神経メカニズムの解明.
2011年度~2014年度, 特別研究員奨励費, 代表, 線虫の温度受容細胞AFDニューロンにおける記憶形成分子メカニズムの解明.
日本学術振興会への採択状況(科学研究費補助金以外)
2017年度~2019年度, 特別研究員, 代表, 記憶の特異性を制御する神経メカニズムの解明.
2011年度~2013年度, 特別研究員, 代表, 線虫の温度受容細胞AFDニューロンにおける記憶形成分子メカニズムの解明.
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九大関連コンテンツ
QIR 九州大学学術情報リポジトリ システム情報科学研究院
理学部・理学研究院
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