Kyushu University Academic Staff Educational and Research Activities Database
List of Papers
Sakuma Shinya Last modified date:2021.07.28

Associate Professor / Department of Mechanical Engineering / Faculty of Engineering


Papers
1. Y. Kasai, C. Leipe, M. Saito, H. Kitagawa, S. Lauterbach, A. Brauer, P. E. Tarasov, T. Goslar, F. Arai, S. Sakuma, Breakthrough in purification of fossil pollen for dating of sediments by a new large-particle on-chip sorter, Science Advances, 10.1126/sciadv.abe7327, 7, 16, Article number: eabe7327, 12 pages, 2021.04.
2. Shinya Sakuma, Hiroki Kumon, Sou Nakamura, Yusuke Kasai, Koji Eto, Fumihito Arai, Three‑dimensional microchannel reflecting cell size distribution for on‑chip production of platelet‑like particles, Microfluidics and Nanofluidics, 10.1007/s10404-021-02433-y, 25, Article number: 36, 12 pages, 2021.03.
3. Tasuku Sato, Shinya Sakuma, Masato Hijikuro, Shingo Maeda, Masayuki Anyoji, Yoko Yamanishi, Design of Electrohydrodynamic Devices with Consideration of Electrostatic Energy, Cyborg and Bionic Systems, 10.34133/2021/5158282, 2021, Article ID 5158282, 8 pages, 2021.01.
4. Nao Nitta, Takanori Iino, Akihiro Isozaki, Mai Yamagishi, Yasutaka Kitahama, Shinya Sakuma, Yuta Suzuki, Hiroshi Tezuka, Minoru Oikawa, Fumihito Arai, Takuya Asai, Dinghuan Deng, Hideya Fukuzawa, Misa Hase, Tomohisa Hasunuma, Takeshi Hayakawa, Kei Hiraki, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Yuki Inoue, Takuro Ito, Masataka Kajikawa, Hiroshi Karakawa, Yusuke Kasai, Yuichi Kato, Hirofumi Kobayashi, Cheng Lei, Satoshi Matsusaka, Hideharu Mikami, Atsuhiro Nakagawa, Keiji Numata, Tadataka Ota, Takeichiro Sekiya, Kiyotaka Shiba, Yoshitaka Shirasaki, Nobutake Suzuki, Shunji Tanaka, Shunnosuke Ueno, Hiroshi Watarai, Takashi Yamano, Masayuki Yazawa, Yusuke Yonamine, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Keisuke Goda, Raman image-activated cell sorting, Nature Communications, 10.1038/s41467-020-17285-3, 11, Article number: 3452, 16 pages, 2020.07, The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to ~100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies..
5. Akihiro Isozaki, Hideharu Mikami, Hiroshi Tezuka, Hiroki Matsumura, Kangrui Huang, Marino Akamine, Kotaro Hiramatsu, Takanori Iino, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Yan Li, Yuta Nakagawa, Shinsuke Ohnuki, Tadataka Ota, Yong Qian, Shinya Sakuma, Takeichiro Sekiya, Yoshitaka Shirasaki, Nobutake Suzuki, Ehsen Tayyabi, Tsubasa Wakamiya, Muzhen Xu, Mai Yamagishi, Haochen Yan, Qiang Yu, Sheng Yan, Dan Yuan, Wei Zhang, Yaqi Zhao, Fumihito Arai, Robert E. Campbell, Christophe Danelon, Dino Di Carlo, Kei Hiraki, Yu Hoshino, Yoichiroh Hosokawa, Mary Inaba, Atsuhiro Nakagawa, Yoshikazu Ohya, Minoru Oikawa, Sotaro Uemura, Yasuyuki Ozeki, Takeaki Sugimura, Nao Nitta, Keisuke Goda, Intelligent image-activated cell sorting 2.0, Lab on a chip, 10.1039/D0LC00080A, 20, 13, 2263-2273, 2020.05, The advent of intelligent image-activated cell sorting (iIACS) has enabled high-throughput intelligent image-based sorting of single live cells from heterogeneous populations. iIACS is an on-chip microfluidic technology that builds on a seamless integration of a high-throughput fluorescence microscope, cell focuser, cell sorter, and deep neural network on a hybrid software–hardware data management architecture, thereby providing the combined merits of optical microscopy, fluorescence-activated cell sorting (FACS), and deep learning. Here we report an iIACS machine that far surpasses the state-of-the-art iIACS machine in system performance in order to expand the range of applications and discoveries enabled by the technology. Specifically, it provides a high throughput of ∼2000 events per second and a high sensitivity of ∼50 molecules of equivalent soluble fluorophores (MESFs), both of which are 20 times superior to those achieved in previous reports. This is made possible by employing (i) an image-sensor-based optomechanical flow imaging method known as virtual-freezing fluorescence imaging and (ii) a real-time intelligent image processor on an 8-PC server equipped with 8 multi-core CPUs and GPUs for intelligent decision-making, in order to significantly boost the imaging performance and computational power of the iIACS machine. We characterize the iIACS machine with fluorescent particles and various cell types and show that the performance of the iIACS machine is close to its achievable design specification. Equipped with the improved capabilities, this new generation of the iIACS technology holds promise for diverse applications in immunology, microbiology, stem cell biology, cancer biology, pathology, and synthetic biology..
6. Takanori Iino, Kazunori Okano, Sang Wook Lee, Takeshi Yamakawa, Hiroki Hagihara, Hong Zhen-Yi, Takanori Maeno, Yusuke Kasai, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai, Yasuyuki Ozeki, Keisuke Goda, Yoichiroh Hosokawa, High-speed microparticle isolation unlimited by Poisson statistics, Lab on a Chip, 10.1039/C9LC00324J, 19, 2669-2677, 2019.07.
7. Yuta Suzuki, Koya Kobayashi, Yoshifumi Wakisaka, Dinghuan Deng, Shunji Tanaka, Chun-Jung Huang, Cheng Leib, Chia-Wei Sun, Hanqin Liu, Yasuhiro Fujiwaki, Sangwook Lee, Akihiro Isozaki, Yusuke Kasai, Takeshi Hayakawa, Shinya Sakuma, Fumihito Arai, Kenichi Koizumi, Hiroshi Tezuka, Mary Inaba, Kei Hiraki, Takuro Ito, Misa Hase, Satoshi Matsusaka, Kiyotaka Shiba, Kanako Suga, Masako Nishikawa, Masahiro Jona, Yutaka Yatomi, Yalikun Yaxiaer, Yo Tanaka, Takeaki Sugimura, Nao Nitta, Keisuke Goda, Yasuyuki Ozeki, Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering, Proceedings of the National Academy of Sciences of the United States of America (PNAS), 10.1073/pnas.1902322116, 116, 32, 15842-15848, 2019.07.
8. Akihiro Isozaki, Hideharu Mikami, Kotaro Hiramatsu, Shinya Sakuma, Yusuke Kasai, Takanori Iino, Takashi Yamano, Atsushi Yasumoto, Yusuke Oguchi, Nobutake Suzuki, Yoshitaka Shirasaki, Taichiro Endo, Takuro Ito, Kei Hiraki, Makoto Yamada, Satoshi Matsusaka, Takeshi Hayakawa, Hideya Fukuzawa, Yutaka Yatomi, Fumihito Arai, Dino Di Carlo, Atsuhiro Nakagawa, Yu Hoshino, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Nao Nitta, Keisuke Goda, A practical guide to intelligent image-activated cell sorting, Nature Protocol, 10.1038/s41596-019-0183-1, 14, 2370-2415, 2019.07.
9. Shinya Sakuma, Ko Nakahara, Fumihito Arai, Continuous mechanical indexing of single-cell spheroids using a robot-integrated microfluidic chip, IEEE Robotics and Automation Letters, 10.1109/LRA.2019.2923976, 4, 3, 2973-2980, 2019.06.
10. Yusuke Kasai, Shinya Sakuma, Fumihito Arai, High-speed on-chip mixing by microvortex generated by controlling local jet flow using dual membrane pumps, IEEE Robotics and Automation Letters, 10.1109/LRA.2019.2921696, 4, 3, 2839-2846, 2019.06.
11. Kotaro Hiramatsu, Takuro Ideguchi, Yusuke Yonamine, SangWook Lee, Yizhi Luo, Kazuki Hashimoto, Takuro Ito, Misa Hase, Jee-Woong Park, Yusuke Kasai, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai, Yu Hoshino, Keisuke Goda, High-throughput label-free molecular fingerprinting flow cytometry, Science Advances, 10.1126/sciadv.aau0241, 5, 1, Article number: eaau0241, 8 pages, 2019.01.
12. Yusuke Kasai, Shinya Sakuma, Fumihito Arai, Isolation of single motile cells using a high‑speed picoliter pipette, Microfluidics and Nanofluidics, 10.1007/s10404-018-2183-9, 23, 2, Articles number: 18, 9 pages, 2019.01.
13. Hiroki Umeshima, Ken-ichi Nomura, Shuhei Yoshikawa, Marcel Hörning, Motomu Tanaka, Shinya Sakuma, Fumihito Arai, Local traction force in the proximal leading process triggers nuclear translocation during neuronal migration, Neuroscience Research, 10.1016/j.neures.2018.04.001, 142, 38-48, 2019.05.
14. Nao Nitta, Takeaki Sugimura, Akihiro Isozaki, Hideharu Mikami, Kei Hiraki, Shinya Sakuma, Takanori Iino, Fumihito Arai, Taichiro Endo, Yasuhiro Fujiwaki, Hideya Fukuzawa, Misa Hase, Takeshi Hayakawa, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Kenichi Koizumi, SangWook Lee, Cheng Lei, Ming Li, Takanori Maeno, Satoshi Matsusaka, Daichi Murakami, Atsuhiro Nakagawa, Yusuke Oguchi, Minoru Oikawa, Tadataka Ota, Kiyotaka Shiba, Hirofumi Shintaku, Yoshitaka Shirasaki, Kanako Suga, Yuta Suzuki, Nobutake Suzuki, Yo Tanaka, Hiroshi Tezuka, Chihana Toyokawa, Yaxiaer Yalikun, Makoto Yamada, Mai Yamagishi, Takashi Yamano, Atsushi Yasumoto, Yutaka Yatomi, Masayuki Yazawa, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Yasuyuki Ozeki, Keisuke Goda, Intelligent Image-Activated Cell Sorting, Cell, 10.1016/j.cell.2018.08.028, 175, 1, 266-276, 266-276.e13, 2018.09.
15. Takuro Ideguchi, Tasuku Nakamura, Shigekazu Takizawa, Miu Tamamitsu, Sangwook Lee, Kotaro Hiramatsu, Venkata Ramaiah-Badarla, Jee-woong Park, Yusuke Kasai, Takeshi Hayakawa, Shinya Sakuma, Fumihito Arai, Keisuke Goda, Microfluidic single-particle chemical analyzer with dual-comb coherent Raman spectroscopy, Optics Letters, 10.1364/OL.43.004057, 43, 16, 4057-4060, 2018.08.
16. Yukitaka Ito, Sou Nakamura, Naoshi Sugimoto, Tomohiro Shigemori, Yoshikazu Kato, Mikiko Ohno, Shinya Sakuma, Keitaro Ito, Hiroki Kumon, Hidenori Hirose, Haruki Okamoto, Masayuki Nogawa, Mio Iwasaki, Shunsuke Kihara, Kosuke Fujio, Takuya Matsumoto, Natsumi Higashi, Kazuya Hashimoto, Akira Sawaguchi, Ken-ichi Harimoto, Masato Nakagawa, Takuya Yamamoto, Makoto Handa, Naohide Watanabe, Eiichiro Nishi, Fumihito Arai, Satoshi Nishimura, Koji Eto, Turbulence Activates Platelet Biogenesis to Enable Clinical Scale Ex Vivo Production, Cell, 10.1016/j.cell.2018.06.011, 174, 3, 636-648, 636-648.e18, 2018.07.
17. Kou Nakahara, Shinya Sakuma, Manabu Kawahara, Masashi Takahashi, Fumihito Arai, Time-Lapse Mechanical Characterization of Zona Pellucida Using a Cell Carrier Chip, Journal of Microelectromechanical Systems, 10.1109/JMEMS.2018.2818183, 27, 3, 464-471, 2018.06.
18. Di Chang, Shinya Sakuma, Kota Kera, Nobuyuki Uozumi, Fumihito Arai, Measurement of the mechanical properties of single Synechocystis sp. strain PCC6803 cells in different osmotic concentrations using a robot integrated microfluidic chip, Lab on a chip, 10.1039/C7LC01245D, 18, 8, 1241-1249, 2018.04.
19. Shinya Sakuma, Ayaka Sato, Nobuhiko Kojima, Fumiya Tao, Fumihito Arai, Force sensor probe using quartz crystal resonator with wide measurement range for mechanical characterization of HepG2 spheroid, Sensors and Actuators A: Physical, 10.1016/j.sna.2017.08.033, 265, 202-210, 2017.10.
20. Shinya Sakuma, Yusuke Kasai, Takeshi Hayakawa, Fumihito Arai, On-chip cell sorting by high-speed local-flow control using dual membrane pumps, Lab on a chip, 10.1039/C7LC00536A, 17, 16, 2685-2884, 2017.01.
21. Hirotaka Sugiura, Shinya Sakuma, Makoto Kaneko, Fumihito Arai, Large indentation method to measure elasticity of cell in robot-integrated microfluidic chip, IEEE Robotics and Automation Letters, 10.1109/LRA.2017.2717500, 2, 4, 2002-2007, 2017.06.
22. Yuichi Murozaki, Shinya Sakuma, Fumihito Arai, Improvement of the measurement range and temperature characteristics of a load sensor using a quartz crystal resonator with all crystal layer components, Sensors, 10.3390/s17051067, 17, 5, Article number: 1067, 13 pages, 2017.05.
23. Utako Yokoyama, Yuta Tonooka, Ryoma Koretake, Taisuke Akimoto, Yuki Gonda, Junichi Saito, Masanari Umemura, Takayuki Fujita, Shinya Sakuma, Fumihito Arai, Makoto Kaneko, Yoshihiro Ishikawa, Arterial graft with elastic layer structure grown from cells, Scientific Reports, 10.1038/s41598-017-00237-1, 7, Article number: 140, 16 pages, 2017.03.
24. Hiroaki Ito, Ryo Murakami, Shinya Sakuma, Chia-Hung Dylan Tsai, Thomas Gutsmann, Klaus Brandenburg, Johannes M. B. Pöschl, Fumihito Arai, Makoto Kaneko, Motomu Tanaka, Mechanical diagnosis of human erythrocytes by ultra-high speed manipulation unraveled critical time window for global cytoskeletal remodeling, Scientific Reports, 10.1038/srep43134, 7, Article number: 43134, 14 pages, 2017.02.
25. Keitaro Ito, Shinya Sakuma, Masaki Kimura, Takanori Takebe, Makoto Kaneko, Fumihito Arai, Temporal transition of mechanical characteristics of HUVEC/MSC spheroids using a microfluidic chip with force sensor probes, Micromachines, 10.3390/mi7120221, 7, 12, Article number: 221, 14 pages, 2016.12.
26. Ryo Murakami, Chia-Hung Dylan Tsai, Makoto Kaneko, Shinya Sakuma, Fumihito Arai, Cell pinball: phenomenon and mechanism of inertia-like cell motion in a microfluidic channel, Lab on a Chip, 10.1039/C5LC00535C, 15, 3307-3313, 2015.07.
27. Hirotaka Sugiura, Shinya Sakuma, Makoto Kaneko, Fumihito Arai, On-chip method to measure mechanical characteristics of a single cell by using moiré fringe, Micromachines, 10.3390/mi6060660, 6, 6, 660-673, 2015.06.
28. Kou Nakahara, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai, On-Chip transportation and measurement of mechanical characteristics of oocytes in an open environment, Micromachines, 10.3390/mi6050648, 6, 5, 648-659, 2015.05.
29. Takeshi Hayakawa, Shinya Sakuma, Fumihito Arai, On-chip 3D rotation of oocyte based on a vibration-induced local whirling flow, Microsystems & Nanoengineering, 10.1038/micronano.2015.1, 1, Article number: 15001, 9 pages, 2015.05.
30. Takumi Monzawa, Makoto Kaneko, Chia-Hung Dylan Tsai, Shinya Sakuma, Fumihito Arai, On-chip actuation transmitter for enhancing the dynamic response of cell manipulation using a macro-scale pump, Biomicrofluidics, 10.1063/1.4907757, 9, Article number: 014114, 10 pages, 2015.02.
31. Shinya Sakuma, Keisuke Kuroda, Fumihito Arai, Tatsunori Taniguchi, Tomohito Ohtani, Yasushi Sakata, Makoto Kaneko, High resolution cell positioning based on a flow reduction mechanism for enhancing deformability mapping, Micromachines, 10.3390/mi5041188, 5, 4, 1188-1201, 2014.11.
32. Takeshi Hayakawa, Shinya Sakuma, Takeshi Fukuhara, Yoshiyuki Yokoyama, Fumihito Arai, A single cell extraction chip using vibration-induced whirling flow and a thermo-responsive gel pattern, Micromachines, 10.3390/mi5030681, 5, 3, 681-696, 2014.09.
33. Chia-Hung Dylan Tsai, Shinya Sakuma, Fumihito Arai, Tatsunori Taniguchi, Tomohito Ohtani, Yasushi Sakata, Makoto Kaneko, Geometrical alignment for improving cell evaluation in a microchannel with application on multiple myeloma red blood cells, RSC Advances, 10.1039/C4RA08276A, 4, 45050-45058, 2014.09.
34. Keitaro Ito, Shinya Sakuma, Yoshiyuki Yokoyama, Fumihito Arai, On-chip gel-valve using photoprocessable thermoresponsive gel, ROBOMECH Journal, 10.1186/s40648-014-0005-8, 1, Article number: 5, 8 pages, 2014.08.
35. Akihiko Ichikawa, Shinya Sakuma, Masakuni Sugita, Tatsuro Shoda, Takahiro Tamakoshi, Satoshi Akagi, Fumihito Arai, On-chip enucleation of an oocyte by untethered microrobots, Journal of Micromechanics and Microengineering, 10.1088/0960-1317/24/9/095004, 24, 9, Article number: 095004, 8 pages, 2014.07.
36. Shinya Sakuma, Keisuke Kuroda, Chia-Hung Dylan Tsai, Wataru Fukui, Fumihito Arai, Makoto Kaneko, Red blood cell fatigue evaluation based on the close-encountering point between extensibility and recoverability, Lab on a Chip, 10.1039/C3LC51003D, 14, 1135-1141, 2014.01.
37. Chia-Hung Dylan Tsai, Shinya Sakuma, Fumihito Arai, Makoto Kaneko, A new dimensionless index for evaluating cell stiffness-based deformability in microchannel, IEEE Transactions on Biomedical Engineering, 10.1109/TBME.2013.2296624, 61, 4, 1187-1195, 2014.01.
38. Shinya Sakuma, Masakuni Sugita, Fumihito Arai, Fabrication of nanopillar micropatterns by hybrid mask lithography for surface-directed liquid flow, Micromachines, 10.3390/mi4020232, 4, 2, 232-242, 2013.06.
39. Hiroki Kuriki, Yoko Yamanishi, Shinya Sakuma, Satoshi Akagi, Fumihito Arai, Local ablation of a single cell using micro/nano bubbles, Journal of Robotics and Mechatronics, 10.20965/jrm.2013.p0476, 25, 3, 476-483, 2013.06.
40. Shinya Sakuma, Fumihito Arai, Cellular force measurement using a nanometric-probe-integrated microfluidic chip with a displacement reduction mechanism, Journal of Robotics and Mechatronics, 10.20965/jrm.2013.p0277, 25, 2, 277-284, 2013.04.
41. Yoko Yamanishi, Shinya Sakuma, Tomohiro Iyanagi, Fumihito Arai, Tatsuo Arai, Akiyuki Hasegawa, Tamio Tanikawa, Akihiko Ichikawa, Osamu Satoh, Akihiro Nakayama, Hiroshi Aso, Mitsuhiro Goto, Seiya Takahashi, Kazutsugu Matsukawa, Design and fabrication of all-in-one unified microfluidic chip for automation of embryonic cell manipulation, Journal of Robotics and Mechatronics, 10.20965/jrm.2010.p0371, 22, 3, 371-379, 2010.06.
42. Takehito Mizunuma, Yoko Yamanishi, Shinya Sakuma, Hisataka Maruyama, Fumihito Arai, Disposable inkjet mechanism for microdroplet dispensing, Journal of Robotics and Mechatronics, 10.20965/jrm.2010.p0341, 22, 3, 341-347, 2010.06.
43. Hisataka Maruyama, Shinya Sakuma, Yoko Yamanishi, Fumihito Arai, Size-dependent filtration and trapping of microparticles in a microfluidic chip using graduated gaps and centrifugal force, Journal of Robotics and Mechatronics, doi: 10.20965/jrm.2010.p0280, 22, 3, 280-285, 2010.06.
44. Yoko Yamanishi, Shinya Sakuma, Kazuhisa Onda, Fumihito Arai, Powerful actuation of magnetized microtools by focused magnetic field for particle sorting in a chip, Biomedical Microdevices, 10.1007/s10544-010-9428-z, 12, 745-752, 2010.05.
45. Yoko Yamanishi, Shinya Sakuma, Yuki Kihara, Fumihito Arai, Fabrication and application of 3-D magnetically driven microtools, Journal of Microelectromechanical Systems, 10.1109/JMEMS.2010.2041188, 19, 2, 350-356, 2010.03.
46. Yoko Yamanishi, Yuki Kihara, Shinya Sakuma, Fumihito Arai, On-chip droplet dispensing by a magnetically driven microtool, Journal of Robotics and Mechatronics, 10.20965/jrm.2009.p0229, 21, 2, 229-235, 2009.06.
47. Yoko Yamanishi, Yuki Kihara, Shinya Sakuma, Fumihito Arai, On-demand production of emulsion droplets using magnetically driven microtool, International Journal of Automation Technology, 10.20965/ijat.2009.p0502, 3, 5, 502-508, 2009.09.
48. Shinya Sakuma, Yoko Yamanishi, Fumihito Arai, Magnetically driven microtools actuated by a focused magnetic field for separating of microparticles, Journal of Robotics and Mechatronics, 10.20965/jrm.2009.p0209, 21, 2, 209-215, 2009.04.
49. Yoko Yamanishi, Shinya Sakuma, Kazuhisa Onda, Fumihito Arai, Biocompatible polymeric magnetically driven microtool for particle sorting, Journal of Micro-Nano Mechatronics, 10.1007/s12213-008-0009-7, 4, 49-57, 2008.07.
50. Yoko Yamanishi, Shinya Sakuma, Fumihito Arai, Production and application of high-accuracy polymer-based magnetically driven microtool, Journal of Robotics and Mechatronics, 10.20965/jrm.2009.p0209, 20, 2, 273-279, 2008.04.