| 1. |
川井 隆之, 超高感度糖鎖分析法の開発（仮題）, 第47回 日本分子生物学会年会@福岡国際会議場, 2024.11. |
| 2. |
川井 隆之, サブセルラー質量分析技術が切り拓く生体膜研究, 日本膜学会第45年会・膜シンポジウム2023合同大会, 2023.11. |
| 3. |
川井 隆之, 超高感度CE-MS分析法による脳分子探査, 第96回日本生化学会大会, 2023.11. |
| 4. |
Takayuki Kawai, Ultra-sensitive Bioanalysis by Dual-Stacking Capillary Electrophoresis, 19th Asia-Pacific International Symposium on Microscale Separations and Analysis 2023 (APCE 2023), 2023.10, Capillary electrophoresis (CE) is an efficient separation technique for biomolecules, however, concentration sensitivity is usually very low because the sample injection volume is quite limited (~nL). To address this issue, online sample preconcentration (OSP) methods have been developed. However, OSP methods often provide only 100-fold or poorer sensitivity improvement in exchange for the loss of resolution, requiring cumbersome optimization for each type of sample. Thus, it is rare to use OSP methods for routine CE bioanalysis. Here, we developed two types of novel OPS methods, large-volume dual preconcentration by isotachophoresis and stacking (LDIS) and large-volume dual preconcentration by micelle collapse and sweeping (LDMS).
LDIS was designed for the analysis of charged molecules, which was firstly applied to N-linked glycans. Owing to the dual stacking mechanism, 2,000-fold sensitivity improvement was achieved without losing the resolution at all. Finally, the around 40 glycans were successfully profiled from quite limited number of cells (~100). LDIS was also applied to metabolome analysis. Single HeLa cells were collected by micro-manipulation and analysed by LDIS-CE-mass spectrometry (MS). Forty metabolites were profiled from a single HeLa cell and limit of detection of 450 fM (10–15 M, S/N = 3) was achieved.
LDMS was designed for uncharged hydrophobic compounds like drugs. DXd, an efficient anti-cancer payload, was extracted from a tissue slice and analysed by LDMS-CE-MS using sodium dodecyl sulfate micelle as pseudo-stationary phase. DXd was trapped by the micelle and eluted by acetonitrile-induced micelle collapse, resulting in 1,000-fold preconcentration and limit of quantification of 420 fM (S/N = 10). Since the hydrophilic metabolites were not trapped by the micelle, DXd was separated from the cellular contaminants, and attomole-level of DXd was successfully quantified from tissue microsections,. |
| 5. |
川井 隆之, 超高感度CE-MS分析技術に基づくドラッグデリバリー創薬支援事業, 九州大学発スタートアップマッチングセミナー, 2023.10. |
| 6. |
川井 隆之, LC/CE二次元分離に基づく超高感度・絶対定量グライコーム解析法の開発, 第74回日本電気泳動学会学術大会, 2023.05. |
| 7. |
川井 隆之, オンライン試料濃縮－キャピラリー電気泳動による超高感度・微量バイオ分析, 大塚電子Webセミナー, 2022.12. |
| 8. |
川井 隆之, 微量脳内分子の網羅解析に向けた超高感度分析技術の開発, 第96回 日本薬理学会年会, 2022.12. |
| 9. |
川井 隆之, 1細胞メタボロミクスへの挑戦, 第95回 日本生化学会大会, 2022.11. |
| 10. |
川井 隆之, 超高感度CE-MS分析プラットフォームの開発と微量バイオ分析への応用, 第42回キャピラリー電気泳動シンポジウム, 2022.10. |
| 11. |
川井 隆之, キャピラリー電気泳動に基づく超高感度N結合糖鎖分析法の開発と組織微小環境分析への応用, 第41回日本糖質学会年会, 2022.09. |
| 12. |
Takayuki Kawai, Development of Ultra-sensitive Capillary Electrophoresis toward Trace N-glycan Profiling, Asian Glyco Webinar, 2022.04. |
| 13. |
川井 隆之, キャピラリー電気泳動における技術開発から応用まで, 第41回キャピラリー電気泳動シンポジウム, 2021.12. |
| 14. |
川井 隆之, キャピラリー電気泳動を駆使した超高感度グライコーム分析法の開発と応用, 第18回JCGGシンポジウム, 2021.12. |
| 15. |
Takayuki Kawai, Development of Ultra-sensitive Capillary Electrophoresis and Application to in vitro/vivo Trace Bioanalysis, Pittcon 2021, 2021.03. |
| 16. |
川井 隆之, 極微量・定量的メタボローム分析技術の開発と応用, 日本質量分析学会 イオン反応研究部会 2020年前期研究会, 2021.02. |
| 17. |
川井 隆之, 超高感度キャピラリー電気泳動法の開発と組織微小環境のオミックス分析, 日本分析化学会 第69年会, 2020.09. |
| 18. |
川井 隆之, 超高感度キャピラリー電気泳動－質量分析法の開発と一細胞メタボローム分析への応用, 日本分析化学会 第80回分析化学討論会, 2020.05. |
| 19. |
川井 隆之, キャピラリー電気泳動の高感度化と微量生体試料分析への応用, 2019年度日本分析化学会近畿支部ぶんせき講習会 (実践編), 2019.07. |
| 20. |
川井 隆之, 超高感度キャピラリー電気泳動を用いた微量オミックス分析, 日本プロテオーム学会2019年大会・第70回日本電気泳動学会総会 合同大会, 2019.07. |
| 21. |
川井 隆之, 高感度キャピラリー電気泳動システムを用いた微量生体試料分析, 分析化学会近畿支部講演会, 2019.07. |
| 22. |
川井 隆之, 超高感度キャピラリー電気泳動を用いた微量糖鎖プロファイリング法, 理研シンポジウム「細胞内糖修飾の統合的ケミカルバイオロジー」, 2019.01. |
| 23. |
川井 隆之, キャピラリー電気泳動を用いた微量生体試料分析, 第38回 キャピラリー電気泳動シンポジウム, 2018.12. |
| 24. |
川井 隆之, 微量質量分析法の開発と応用, 東北大学薬学部セミナー, 2018.11. |
| 25. |
川井 隆之, 超高感度CE-MSを用いた一細胞メタボローム・プロテオーム分析, 第6回バイオ関連シンポジウム 若手フォーラム, 2018.09. |
| 26. |
Takayuki Kawai, Quantitative Single Cell Metabolomics by CE-MS, 17th Asia-Pacific International Symposium on Microscale Separations and Analysis (APCE2017), 2017.11. |
| 27. |
Takayuki Kawai, Ultra-sensitive Capillary Electrophoresis for Single Cell Omics Research, Pittcon 2017, 2017.03. |
| 28. |
川井 隆之, 超高感度CE-MS分析システムによる極微量オミックス解析, 日本化学会 第97春季年会, 2017.03. |
| 29. |
Takayuki Kawai, Ultra-sensitive CE-LIF/MS System for Single Cell Omics Research, 16th Asia-Pacific International Symposium on Microscale Separations and Analysis (APCE2016), 2016.11. |
| 30. |
Takayuki Kawai, High performance CE-MS system for single cell analysis, 32nd International Symposium on Microscale Separations and Bioanalysis (MSB 2016), 2016.04. |
| 31. |
川井 隆之, ミクロスケール空間を利用した高性能バイオ分析法の開発, 2015年度 上智大学物質生命理工学科コロキウム, 2016.01. |
| 32. |
川井 隆之, キャピラリー電気泳動を用いた高感度一細胞分析法の開発, 第35回キャピラリー電気泳動シンポジウム, 2015.11. |
| 33. |
川井 隆之, オンライン試料濃縮法を利用した微量生体試料のCE分析, 第66回日本電気泳動学会総会, 2015.09. |
| 34. |
Takayuki Kawai, Ultra-sensitive capillary electrophoresis for single cell analysis, International Conference and Expo on Separation Techniques 2015, 2015.08. |
| 35. |
Takayuki Kawai, Sensitive and Flexible Single-Cell Analysis via Capillary Electrophoresis Coupled with an Online Sample Preconcentration Method, 1st Caparica Christmas Conference on Sample Treatment (Sample Treatment 2014), 2014.12. |
| 36. |
Takayuki Kawai, Amit Patel, Stanislav S Rubakhin, Jonathan V Sweedler, Analysis of D-Glutamate in a Single Cell via Capillary Electrophoresis Coupled with an Online Sample Preconcentration Method, The 2nd International Conference of D-Amino Acid Research (IDAR 2014), 2014.09. |
| 37. |
Takayuki Kawai, Stanislav S. Rubakhin, Jonathan V. Sweedler, Analysis of Enantiomeric Amino Acids in Biological Samples via Capillary Electrophoresis Coupled with Mass Spectrometry, AS-MS 2014, 2014.06. |
| 38. |
Takayuki Kawai, Simple and High-Performance On-chip Bioanalysis, EMN Summer Meeting 2014, 2014.06. |
| 39. |
Takayuki Kawai, Nobutoshi Ota, Jonathan V. Sweedler, Analysis of Enantiomeric Amino Acids in Biological Samples via Capillary Electrophoresis Coupled with Laser-induced Fluorescence and Mass Spectrometry, Pittcon 2014, 2014.03. |
| 40. |
Takayuki Kawai, Masumi Ueda, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka, Toward 10,000-fold Sensitivity Improvement in Capillary Electrophoresis of Oligosaccharide, 11th Asia-Pacific International Symposium on Microscale Separations and Analysis (APCE 2011), 2011.11. |
| 41. |
Takayuki Kawai, Masumi Ueda, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka, Toward 10,000-fold Sample Preconcentration Efficiency for Oligosaccharide Analysis in Capillary Electrophoresis, 37th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2011 Dalian), 2011.10. |
| 42. |
Takayuki Kawai, Jun Ito, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka, Highly Sensitive Chiral Analysis by Capillary Electrophoresis Using Large-volume Sample Stacking with Electroosmotic Flow Pump, IUPAC International Congress on Analytical Sciences 2011 (ICAS 2011), 2011.05. |
| 43. |
Takayuki Kawai, Jun Ito, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka, Highly Sensitive Chiral Analysis in Capillary Electrophoresis Using Large-volume Sample Stacking with Electroosmotic Flow Pump, 26th International Symposium on Microscale Bioseparations (MSB 2011), 2011.05. |
| 44. |
Takayuki Kawai, Kenji Sueyoshi, Fumihiko Kitagawa, Koji Otsuka, Microchip Electrophoresis of Oligosaccharides with Large-volume Sample Stacking with Electroosmotic Flow Pump in Single Channel, 2010 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010), 2010.12. |
